CN101493458A

CN101493458A - Paraquat-glyphosate dual detecting card and processing method of test sample thereof

Info

Publication number: CN101493458A
Application number: CNA200910042774XA
Authority: CN
Inventors: 周坚
Original assignee: Individual
Current assignee: Individual
Priority date: 2009-03-03
Filing date: 2009-03-03
Publication date: 2009-07-29

Abstract

The invention discloses a paraquat-glyphosate bigeminal detection card; the internal part of an outer shell has a test strip which is pasted with a nitrocellulose membrane in the middle of a supporting back plate; the two end parts are respectively pasted with an absorbent membrane and a sample pad; the inner ends are respectively overlapped with one end of the nitrocellulose membrane; a section of colloidal gold film which is a glass fiber membrane containing anti-paraquat and anti-glyphosate monoclonal antibody or polyclonal antibody colloidal gold marker is clamped between the overlapped part of the sample pad and the nitrocellulose membrane; the nitrocellulose membrane has 2 test strips which respectively contain a paraquat protein conjugate and a glyphosate protein conjugate; and a matrix pore area contains an anti-rabbit antibody or anti-mouse antibody. The sample pad and the nitrocellulose membrane respectively face a sampling hole and a detection window hole. The invention has the advantages that the paraquat and glyphosate contained in fruits, vegetables, and cereals can be simultaneously detected; the preparation is easy, the detection cost is saved, the use is convenient, the detection is rapid, the sensitivity is high and the result is accurate.

Description

Paraquat---the disposal route of glyphosate bigeminy test card and test sample thereof

Technical field:

The present invention relates to a kind of utensil that detects pesticide residue, particularly relate to a kind of paraquat---the disposal route of glyphosate bigeminy test card and test sample thereof.

Background technology:

Agricultural chemicals is the special chemicals of a class, and according to the literature, the agricultural chemicals utilization factor is generally 10%, has 90% to remain in the environment approximately, transfers to human body by the inrichment of food chain, and human body is produced harm.Paraquat and glyphosate are the herbicides of using in the world early, and human long-term absorption case fatality rate is very high.In Dec, 2008, the 16 years old boy in Shanghai causes severe intoxication because of eating paraquat by mistake, and by retrieval, " paraquat " case of abroad having reported of being poisoned to death of eating by mistake reaches hundreds of examples, and it is all dead wherein to eat regular dosage form 49 more than 30 milliliters example by mistake.The conduction of glyphosate in plant is strong, also exists certain residual in fruit.Therefore how to detect the residues of pesticides in the agricultural product fast and accurately, for ensureing that people's life safety has great importance.

At present, prior art mainly relies on stratographic analysis, enzyme immunoassay kit for the detection of paraquat and glyphosate, but up to the present, does not detect the appliance of these two kinds of materials simultaneously.

Summary of the invention:

The object of the present invention is to provide a kind of utensil that can detect paraquat and glyphosate simultaneously, overcome the prior art defective of individual inspiration one by one.

Technical scheme of the present invention is a paraquat---the disposal route of glyphosate bigeminy test card and test sample thereof, a kind of paraquat---glyphosate bigeminy test card, it is in the test card shell of rectangular flat shell shape test-strips to be set, the test card case surface has the fenestra of detection and well, it is characterized in that test-strips forms by pasting four kinds of elements on the supporting backboard: stacked stickup nitrocellulose filter in the middle part of the supporting backboard, stacked stickup absorbing membrane on supporting backboard one end, stacked stickup sample pad on the other end, absorbing membrane is inner to overlap with nitrocellulose filter one end respectively separately with sample pad the inner, clinch at sample pad and nitrocellulose filter, insert and put between the two and paste one section collaurum film, the collaurum film is the glass fibre membrane that contains anti-paraquat and resistance glyphosate monoclonal antibody or polyclonal antibody colloid gold label thing, there are 2 to detect band and 1 matter pore area on the nitrocellulose filter, article one, detect band and contain the paraquat protein conjugate, another detects band and contains the glyphosate protein conjugate, and the matter pore area is anti-rabbit antibody or anti-mouse antibody; Test-strips is inserted in the test card shell, and sample pad is over against well, and nitrocellulose filter is over against detecting fenestra.

Adopting above-mentioned paraquat---glyphosate bigeminy test card, test sample can be samples such as vegetables, fruit, cereal, for various test sample different disposal routes is arranged.

Disposal route at fruit, vegetables sample: take by weighing 1 gram fruit, vegetables sample, place the test tube that contains 1ml sample extract A, tighten the pipe lid, leave standstill 10min behind the thermal agitation, draw supernatant liquor and make specimen.

Disposal route at the cereal sample: take by weighing 1 gram cereal sample, place the test tube that contains 1ml sample extract B, tighten the pipe lid, leave standstill 10min behind the thermal agitation, draw supernatant liquor and make specimen.

Advantage of the present invention is to detect paraquat and the glyphosate that contains in fruit, vegetables, the cereal simultaneously, is easy to preparation and saves testing cost, and is easy to use, detects fast, and highly sensitive, the result is accurate.

Description of drawings:

Fig. 1 is a paraquat---glyphosate bigeminy test card shell and test-strips arrangement figure in the enclosure.

Fig. 2 is a paraquat---the detection strip structural representation of glyphosate bigeminy test card.

Fig. 3 detects on the nitrocellulose filter of strip to show the trace synoptic diagram.

Embodiment:

The present invention further specifies as follows in conjunction with specific embodiments referring to accompanying drawing:

A kind of paraquat---glyphosate bigeminy test card, it is that test-strips 2 is set in test card shell 1, the setting of the structure of test card shell 1 and test-strips 2 is referring to accompanying drawing 1, test card shell 1 is rectangular flat shell shape housing, long 7cm, wide 2cm, thick 0.5cm, shell wall thickness 0.1cm is made by engineering plastics.Test card shell 1 is connected with the shell block two halves by cap and constitutes, and the fenestra 3 of detection and well 4 are arranged on test card shell 1 cap.Test-strips 2 is placed in the shell block of test card shell 1.

Test-strips 2 is fillet thin slices of sandwich construction, and sheet is about 6.0cm, the wide about 0.4cm of sheet, and thickness is less than 2.5mm.Test-strips 2 is sandwich constructions: bottom is a supporting backboard 5, is the igelite thin slice, thick about 0.5mm, long 6.0cm, wide 0.4cm; The stacked stickup nitrocellulose filter 6 in supporting backboard 5 middle parts, cellulose nitrate thickness 0.5mm, long 2-2.5cm, wide 0.4cm.Three isolated horizontal demonstration trace bands are arranged on the nitrocellulose filter 6, referring to accompanying drawing 3, article three, show in the trace band, article one, be to detect to be with 10, contain the paraquat protein conjugate, another is to detect to be with 11, contains the glyphosate protein conjugate, article three, be matter pore area 12, contain anti-rabbit antibody or anti-mouse antibody.Stacked stickup absorbing membrane 9 on supporting backboard 5 one ends, stacked stickup sample pad 8 on supporting backboard 5 the other end, absorbing membrane 9 the inners overlap with nitrocellulose filter 6 one ends respectively separately with sample pad 8 the inners, at the clinch of sample pad 8 with nitrocellulose filter 6, insert and put between the two and paste one section collaurum film 7, collaurum film 7 is the glass fibre membranes that contain anti-paraquat and resistance glyphosate monoclonal antibody or polyclonal antibody colloid gold label thing.Collaurum film 7 thick 0.5-0.8mm, long 0.5-0.6cm, wide 0.4cm.Sample pad 8 thick 0.5-0.8mm, long 2cm, wide 0.4cm.Absorbing membrane 9 thick 0.5-0.8mm, long 1.6-2cm, wide 0.4cm.Collaurum film 7, sample pad 8 and absorbing membrane 9 are 0.1-0.2cm with the nitrocellulose filter lap of splice.Absorbing membrane 9 materials are thieving paper, and sample pad 8 materials are water adsorption glass tunica fibrosas.

Test-strips 2 is inserted in the shell 1, and sample pad 8 is over against well 4, and nitrocellulose filter 6 is over against detecting fenestra 3.

Paraquat of the present invention---glyphosate bigeminy test card is used for detecting fruit, vegetables, cereal and whether contains paraquat, glyphosate, differentiates the security of agricultural product in view of the above.

For different test sample, different disposal routes is arranged:

Use paraquat---when glyphosate bigeminy test card detects, earlier sample liquid to be detected is instilled into paraquat from well 4---the sample pad 8 of glyphosate bigeminy test card 2, because syphonic effect principle, drive contained anti-paraquat of sample liquid to be detected and collaurum film 7 and glyphosate monoclonal or polyclonal antibody colloid gold label thing together to nitrocellulose filter 6 diffusions, observations in 5-10 minute.

The key reaction of test card is immunologic antigen and antibody response, the antibody of the colloid gold label that on nitrocellulose filter, moves, on calibration tape respectively with contain paraquat protein conjugate, glyphosate protein conjugate, and anti-rabbit antibody of matter pore area or anti-mouse antibody reaction, form the brownish red band.If have in the sample corresponding paraquat to be checked and glyphosate to exist, after sample adds, promptly react with corresponding antibody, and not can with contained paraquat protein conjugate, the reaction of glyphosate protein conjugate of relevant detection band, thereby do not develop the color.The result of key reaction has following 5 kinds of situations:

If 1 show to detect be with 10,11 and matter pore area 12 manifest brownish red trace band simultaneously, the expression testing result is negative, illustrates not contain paraquat and glyphosate in the detected sample.

Otherwise 2, detect and to be with 10,11 Show Colors not, have only matter pore area 12 to manifest the brownish red trace simultaneously, testing result is positive, shows to contain paraquat and glyphosate in the detected sample simultaneously.

If 3 detect and to be with 10 Show Colors not, detect be with 11 and matter pore area 12 manifest the brownish red trace simultaneously, testing result is positive, shows in the detected sample to contain paraquat.

If 4 detect and to be with 11 Show Colors not, detect be with 10 and matter pore area 12 manifest the brownish red trace simultaneously, testing result is positive, shows in the detected sample to contain glyphosate.

If 5 detect be with 10,11 and matter pore area 12 all do not develop the color, show that then detecting strip lost efficacy.

Claims

1, a kind of paraquat---glyphosate bigeminy test card, it is in the test card shell of rectangular flat shell shape test-strips to be set, the test card case surface has the fenestra of detection and well, it is characterized in that test-strips forms by pasting four kinds of elements on the supporting backboard: stacked stickup nitrocellulose filter in the middle part of the supporting backboard, stacked stickup absorbing membrane on supporting backboard one end, stacked stickup sample pad on the other end, absorbing membrane is inner to overlap with nitrocellulose filter one end respectively separately with sample pad the inner, clinch at sample pad and nitrocellulose filter, insert and put between the two and paste one section collaurum film, the collaurum film is the glass fibre membrane that contains anti-paraquat and resistance glyphosate monoclonal antibody or polyclonal antibody colloid gold label thing, there are 2 to detect band and 1 matter pore area on the nitrocellulose filter, article one, detect band and contain the paraquat protein conjugate, another detects band and contains the glyphosate protein conjugate, and the matter pore area is anti-rabbit antibody or anti-mouse antibody; Test-strips is inserted in the test card shell, and sample pad is over against well, and nitrocellulose filter is over against detecting fenestra.

2, according to the paraquat described in the claim 1---glyphosate bigeminy test card, the disposal route of its various test sample is:

Disposal route at fruit, vegetables sample: take by weighing 1 gram fruit, vegetables sample, place the test tube that contains 1ml sample extract A, tighten the pipe lid, leave standstill 10min behind the thermal agitation, draw supernatant liquor and make specimen;