CN202710570U - Kit for fast detecting patulin - Google Patents
Kit for fast detecting patulin Download PDFInfo
- Publication number
- CN202710570U CN202710570U CN 201220291285 CN201220291285U CN202710570U CN 202710570 U CN202710570 U CN 202710570U CN 201220291285 CN201220291285 CN 201220291285 CN 201220291285 U CN201220291285 U CN 201220291285U CN 202710570 U CN202710570 U CN 202710570U
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- box body
- patulin
- kit
- lid
- colloidal gold
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Abstract
The utility model relates to a kit for fast detecting patulin and belongs to medical detection supplies. The kit is characterized in that a test item sign is arranged between a sample feed hole and a result observation hole, a pair of opposite angles of the upper and lower surfaces of a closed box body are in arc shapes, air vents are arranged on the closed box body, a colloidal gold labeling patulin monoclonal antibody (McAb) is coated on a gold labeling fiber pad of a colloidal gold test strip, and a patulin-bovine serum albumin (BSA) conjugate and a rabbit anti-mouse IgG polyclonal antibody are respectively coated on a detection line T and a control line C of a cellulose emulsion of the colloidal gold test strip. The patulin in fruit food is fast detected through the competitive inhibition principle and the colloidal gold labeling technique; and the kit is simple, sensitive, accurate, peculiar, easy to store, convenient to use, attractive and elegant.
Description
Technical field
The utility model relates to field of biological detection, particularly relates to a kind of kit of fast detecting patulin.
Background technology
Patulin (Patulin, Pat) is the secondary metabolite of aspergillus, mould and the mould generation of silk clothes, and animal is had teratogenesis, mutagenesis and carcinogenicity, extensively is present in the food and feed, and patulin has caused people's attention to the harm of animal.Patulin mainly pollutes apple, hawthorn and juice product thereof, and has become an important indicator judging the apple juice quality.The patulin pollutant is considered to the world today and jeopardizes one of five large toxin of human food's safety.The highest allowance of patulin is 50 μ g/kg in national regulation apple, the haw productses such as Chinese and EU.
At present, the detection method of patulin has thin-layered chromatography (TLC), vapor-phase chromatography (GC), high performance liquid chromatography (HPLC) etc., although highly sensitive, Sample pretreatment and measurement operation are loaded down with trivial details, and expense is high, is unfavorable for applying; And the enzyme linked immunosorbent detection method although detection limit is lower, detects forward and backward all need to do necessary instrument cleaning and maintenance work, and detection time is long, and requires this area professional and technical personnel to operate.
Immune colloidal gold technique (Immunogold labelling techique) is the solid phase labelling immunoassay that grows up after fluorescein, radioactive isotope and enzyme three large labelling techniques the eighties in last century.This technology has mainly utilized gold grain to have the characteristic of high electron density, when these labels are assembled in a large number at corresponding part place, forms macroscopic redness or pink spot, thereby is used for qualitative or semiquantitative tachysynthesis detection method.Fast diagnose test paper bar is a novel vitro diagnostic techniques that has grown up on monoclonal antibody technique, colloidal gold immunochromatographimethod technology and novel chromatographic material basis since the nineties in 20th century, development in recent years is rapid, particularly is widely applied in the medical test at biomedical sector.This technology mainly is that specific antigen or antibody are fixed on the nitrocellulose membrane with ribbon, colloid gold label reagent is adsorbed on the pad, be added on the sample pad of test strips one end when testing sample after, move forward by capillary action, react to each other behind the colloid gold label reagent on the dissolving pad, when moving to again fixing antigen or antibody regions, specific binding occurs with it and is trapped in determinand gold marked reagent compound, colloid gold label is gathered in to detect and is with, can be by the range estimation result that developed the color intuitively.Free label is then crossed and is detected band, thereby reaches the purpose of automatically separating with the detection thing.
A competitive inhibition method can be used for measuring antigen or antibody, to measure antigen as example, its principle is that the envelope antigen competition of being examined antigen and solid phase in the sample is combined with labelled antibody, the amount of being examined antigen in sample is more than or equal to the threshold value of reagent, and labelled antibody then can be combined with the envelope antigen of solid phase; If without being examined antigen, then labelled antibody directly is combined with the envelope antigen of solid phase in the sample.
The immune colloidal gold chromatography technical operation is simple, fast, sensitivity and specificity be good, the fast detecting that is used for patulin, although technique is a kind of comparatively proven technique, but colloidal gold strip is polluted easily, bad preservation is so that exist foot how inconvenient in detection and use observation process.
The utility model content
Be demand and the blank that solves above-mentioned field, the utility model aims to provide a kind of kit of fast detecting patulin, be used for the detection of fruit type food patulin, the utility model kit adopts A competitive inhibition method and the immune colloidal gold technique fast detecting patulin that combines.
A kind of kit of fast detecting patulin comprises the box obturator that box body, lid consist of and places colloidal gold strip in the box body, has well, test item sign and viewport as a result on the described lid; It is characterized in that: described test item sign is located at described well and described as a result between the viewport, the a pair of diagonal angle of described box obturator upper and lower surface is arc, also be provided with bleeder vent on the described box obturator, the gold mark fiber mat of described colloidal gold strip is coated with the patulin monoclonal antibody of colloid gold label, is coated with respectively patulin-bovine serum albumin BSA conjugate and the anti-mouse IgG of rabbit on the detection line T line on the cellulose medicine film of described colloidal gold strip and the control line C line and resists more.
Described bleeder vent is located at described box obturator lid one side.
Described bleeder vent is a plurality of, is fan-shaped array.
Being provided with the locating slot that colloidal gold strip is installed in the described box body, when described colloidal gold strip is positioned over locating slot in the box body, is the sample fiber pad corresponding to lid well place; Being gold mark fiber mat corresponding to test item sign place, is cellulose medicine film corresponding to described as a result viewport place.
Described box body is provided with coupling arrangement, and described lid is connected the detachable connection of coupling arrangement with box body.
One side corresponding to box body on the described lid is provided with a plurality of apertures, and described box body is provided with projection with described box body one corresponding to the one side of lid, and the aperture population size on described projection and the described lid is suitable.
Described box body is provided with sample number into spectrum.
Described sample number into spectrum is located at described box obturator lid one side, and a side of close described as a result viewport.
Technique effect:
The kit of fast detecting patulin of the present utility model comprises the box obturator that box body, lid consist of and places colloidal gold strip in the box body, has well, test item sign and viewport as a result on the described lid; It is characterized in that: described test item sign is located at described well and described as a result between the viewport, be convenient to observe and use, be convenient to identify concrete test item, the a pair of diagonal angle of described box obturator upper and lower surface is arc, save material space, and elegant in appearance, also be provided with bleeder vent on the described box obturator, so that can keep air-flow to communicate with the external world in the box body, when detecting, guarantee that reaction film normally brings into play capillarity, make detect thing can be fully rapidly displacement to ventilative nose end, avoid the phenomenons such as retardance or incomplete swimming, guaranteed the sensitivity that detects, accuracy and high-level efficiency.The gold mark fiber mat of described colloidal gold strip is coated with the patulin monoclonal antibody of colloid gold label for patulin antigen coated on the patulin of identifying sample or the detection line T line-bovine serum albumin BSA conjugate, be coated with respectively patulin antigen-bovine serum albumin BSA conjugate on set detection line T line and the control line C line on the cellulose medicine film of described colloidal gold strip and how anti-the anti-mouse IgG of rabbit is, be used for the colour developing of concrete specific reaction.
The utility model is preferably opened bleeder vent on described lid.Preventing from detecting liquid out dyes on operating personnel's the skin from the bleeder vent water clock.
The further preferred bleeder vent of the utility model is a plurality of apertures, is fan-shaped array.The design of a plurality of apertures makes air hole both play the effect of ventilation, can not allow again reaction film come out, thereby avoid polluteing Person's skin.Fan-shaped array is comparatively attractive in appearance.
Be provided with the locating slot that colloidal gold strip is installed in the box body described in the utility model, when described colloidal gold strip is positioned over locating slot in the box body, be the sample fiber pad corresponding to lid well place, being gold mark fiber mat corresponding to test item sign place, is cellulose medicine film corresponding to described as a result viewport place.
Described box obturator is provided with coupling arrangement, and described lid is connected the detachable connection of coupling arrangement with box body, on the one hand convenient use, and box body and lid are reusable on the other hand, saving resource.
One side corresponding to box body on the described lid is provided with a plurality of apertures, described box body is provided with projection with described box body one corresponding to the one side of lid, and the aperture population size on described projection and the described lid is suitable, and processing and fabricating is simple, for convenience detach, be difficult for polluting.
Also be provided with sample number into spectrum on the described box obturator, be convenient to search, distinguish, be convenient to identify product category and test event.
The preferred described sample number into spectrum of the utility model is located at described box obturator lid one side, and a side of close described as a result viewport, easy to use and observation.
The utility model is simple to operate, easy to use, bring namely and use, do not need one of skill in the art, without any need for instrument, can satisfy different levels personnel's needs, spread to easily food service industry and average family, show the result with symbol, broken away from the specific (special) requirements to equipment, instrument, personnel and storage and transportation environment, and had quick, easy, sensitive, accurate, special characteristics.
Description of drawings
Fig. 1 the utility model kit lid synoptic diagram
Fig. 2 the utility model kit preferred version lid synoptic diagram
Fig. 3 the utility model kit synoptic diagram
Fig. 4 the utility model kit preferred version synoptic diagram
Fig. 5 the utility model kit box body synoptic diagram
Fig. 6 the utility model colloidal gold strip synoptic diagram
1-lid wherein, the 2-box body, the 3-well, 4-test item sign, 5-is viewport as a result, 6-box obturator, 7-sample number into spectrum, 8-colloidal gold strip, 9-projection, 10-sample fiber pad, 11-gold mark fiber mat, 12-cellulose medicine film, 13-water-absorption material backing strap, 14-bleeder vent.
Embodiment:
It is in order further to understand better the utility model that following embodiment is provided; be not limited to described preferred forms; content of the present utility model and protection domain are not construed as limiting; anyone makes up with the feature of other prior aries under enlightenment of the present utility model or with the utility model and the identical or akin product of any and the utility model that draws, all drops within the protection domain of the present utility model.
The utility model is simple to operate, easy to use, and is elegant in appearance, with low cost, has quick, accurate, convenient, inexpensive characteristics.
Pat refers to that patulin indicates item in the Figure of description.
The kit of fast detecting patulin of the present utility model comprises the box obturator 6 that box body 2, lid 1 consists of and places colloidal gold strip 8 in the box body 2, has well 3, test item sign 4 and viewport 5 as a result on the described lid 1; It is characterized in that: described test item sign 4 is located between described well 3 and the described as a result viewport 5, be convenient to observe and use, be convenient to identify concrete test item, the a pair of diagonal angle of described box obturator 6 upper and lower surfaces is arc, save material space, and elegant in appearance, also be provided with bleeder vent 14 on the described box obturator 6, so that can keep air-flow to communicate with the external world in the box body 2, when detecting, guarantee that reaction film normally brings into play capillarity, make detect thing can be fully rapidly displacement to bleeder vent 14 ends, avoid the phenomenons such as retardance or incomplete swimming, guaranteed the sensitivity that detects, accuracy and high-level efficiency.The patulin monoclonal antibody that the gold mark fiber mat 11 of described colloidal gold strip 8 is coated with colloid gold label be used for the patulin of liquid to be checked or detection line T line on coated patulin coupled antigen carry out specific binding, be coated with respectively patulin antigen-bovine serum albumin BSA conjugate on set detection line T line and the control line C line on the cellulose medicine film 12 of described colloidal gold strip 8 and how anti-the anti-mouse IgG of rabbit is, be used for the colour developing of concrete specific reaction.
The utility model is preferably opened bleeder vent 14 on described lid 1.Control detects liquid and out dyes on operating personnel's the skin from bleeder vent 14 water clocks.
The further preferred bleeder vent 14 of the utility model is a plurality of apertures, is fan-shaped array.The design of a plurality of apertures makes air hole both play the effect of ventilation, can not allow again reaction film come out, thereby avoid polluteing Person's skin.Fan-shaped array is comparatively attractive in appearance.
Be provided with the locating slot that colloidal gold strip 8 is installed in the box body 2 described in the utility model, when described colloidal gold strip 8 is positioned over locating slot in the box body 2,3 places are the sample fiber pad corresponding to lid 1 well, identifying 4 places for gold mark fiber mat 11 corresponding to test item, is cellulose medicine film 12 corresponding to described as a result viewport 5 places.
Described box obturator 6 is provided with coupling arrangement, and described lid 1 is connected with box body by the detachable connection of coupling arrangement, on the one hand convenient use, and box body 2 and lid 1 are reusable on the other hand, saving resource.
One side corresponding to box body 2 on the described lid 1 is provided with a plurality of apertures, described box body 2 is provided with projection with described box body 2 one corresponding to the one side of lid 1, and the aperture population size on described projection and the described lid 1 is suitable, and processing and fabricating is simple, for convenience detach, be difficult for polluting.
Also be provided with sample number into spectrum 7 on the described box obturator 6, be convenient to search, distinguish, be convenient to identify product category and test event.
The preferred described sample number into spectrum 7 of the utility model is located at described box obturator 6 lids 1 one side, and a side of close described as a result viewport 5, easy to use and observation.
The preparation method of experimental example 2. the utility model test strips
The preparation procedure of test strips is as follows
Being coated with of step 1. cellulose medicine film
1, with the anti-mouse lgG of rabbit anti-in contrast line C lines how, patulin-bovine serum albumin BSA conjugate is as detection line T line, and two reagent are respectively according to required concentration (scope 1.5-2mg/ml), dilutes with the PBS of 20mM.
2, with the mentioned reagent for preparing, coated to nitrocellulose filter respectively according to C, T line position, then dry 6 hours, temperature was 30 ℃, humidity≤35%, thus make cellulose medicine film.
The fibrolaminar mark of step 2. gold mark:
1, gets the colloidal gold solution of 40nm, press final concentration 8-12 μ l/ml and add 0.2M K
2CO
3, add certain density patulin monoclonal antibody, placed room temperature reaction 15-30 minute, then add the solution of 20%BSA according to concentration 20 μ l/ml, be used for saturated free collaurum.Last 10000rpm centrifugal 30 minutes, removes unconjugated protein in the supernatant.Colloid gold label stoste with isopyknic 0.5m BAS (containing 1%Tween, 5% sucrose, 1% hydrolysis BSA, 1% caseinhydrolysate, 0.1%PVA) redissolution precipitation.
2, with above-mentioned colloid gold label stoste according to 10-20% concentration, dilute with the 0.5m BAS, thereby be mixed with collaurum working fluid behind the mark.And then this working fluid is laid on the 30*30cm fiberglass packing, dry 6 hours, temperature was 30 ℃, humidity≤35%, thus make gold mark pad.
The assembling of step 3. test strips:
1, plastic plate is lain on the operator's console, stick double sticky tape.
2, tear the double sticky tape lining paper, stick at 30mm place, distance plastic plate upper end and be coated with patulin-bovine serum albumin BSA conjugate and the many anti-nitrocellulose filters of the anti-mouse IgG of rabbit.
3, robust fibre is absorbed water letterweight nitrocellulose filter upper end 2mm sticks, and evens up on upper end and the plastic plate.
4, press 0.5mm place, nitrocellulose filter lower end to stick little nonwoven fabrics.
5, gold mark pad is evened up be pressed on above the little nonwoven fabrics.
6, fiberglass packing is pressed on gold mark pad upper approximately half, stick apart from 2mm place, plastic plate lower end the lower end.
7, large nonwoven fabrics and gold mark pad upper end are evened up, lower end and plastic plate lower end are evened up and are sticked.
8, press nitrocellulose filter 1.5-2mm to stick on the arrow adhesive tape.
9, press robust fibre thieving paper to stick on the handheld terminal adhesive tape.
The kit of a kind of fast detecting patulin of the utility model adopts combine patulin in the fast detecting food of A competitive inhibition method principle and immune colloidal gold technique.The utility model test strips is simple to operate, easy to use, bring namely and use, do not need one of skill in the art, can satisfy different levels personnel's needs, spread to easily food service industry and average family, the utility model shows the result with symbol, break away from the specific (special) requirements to equipment, instrument, personnel and storage and transportation environment, be used for the fast detecting of fruit type food patulin.The utility model kit is simple and direct, sensitive, accurate, and preserves easily, and is easy to use, elegant in appearance.
Claims (6)
1. the kit of a fast detecting patulin comprises the box obturator that box body, lid consist of and places colloidal gold strip in the box body, has well, test item sign and viewport as a result on the described lid; It is characterized in that: described test item sign is located at described well and described as a result between the viewport, the a pair of diagonal angle of described box obturator upper and lower surface is arc, also be provided with bleeder vent on the described box obturator, the gold mark fiber mat of described colloidal gold strip is coated with the patulin monoclonal antibody of colloid gold label, is coated with respectively patulin-bovine serum albumin BSA conjugate and the anti-mouse IgG of rabbit on the detection line T line on the cellulose medicine film of described colloidal gold strip and the control line C line and resists more.
2. the kit of fast detecting patulin according to claim 1 is characterized in that, described bleeder vent is located at described box obturator lid one side.
3. the kit of fast detecting patulin according to claim 2 is characterized in that, described bleeder vent is a plurality of, is fan-shaped array.
4. the kit of fast detecting patulin according to claim 3, it is characterized in that, being provided with the locating slot that colloidal gold strip is installed in the described box body, when described colloidal gold strip is positioned over locating slot in the box body, is the sample fiber pad corresponding to lid well place; Be gold mark fiber mat corresponding to test item sign place; Be cellulose medicine film corresponding to described as a result viewport place.
5. the kit of fast detecting patulin according to claim 4 is characterized in that, described box body is provided with coupling arrangement, and described lid is connected the detachable connection of coupling arrangement with box body.
6. the kit of fast detecting patulin according to claim 5, it is characterized in that, one side corresponding to box body on the described lid is provided with a plurality of apertures, described box body is provided with projection with described box body one corresponding to the one side of lid, and the aperture population size on described projection and the described lid is suitable.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220291285 CN202710570U (en) | 2012-06-15 | 2012-06-15 | Kit for fast detecting patulin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220291285 CN202710570U (en) | 2012-06-15 | 2012-06-15 | Kit for fast detecting patulin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202710570U true CN202710570U (en) | 2013-01-30 |
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ID=47590858
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220291285 Expired - Fee Related CN202710570U (en) | 2012-06-15 | 2012-06-15 | Kit for fast detecting patulin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202710570U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109085159A (en) * | 2017-08-21 | 2018-12-25 | 上海荒岛科技有限公司 | A kind of substrate and detection card |
| CN109085348A (en) * | 2017-08-21 | 2018-12-25 | 上海荒岛科技有限公司 | A kind of method for analyzing body fluid |
| ES2849398A1 (en) * | 2020-02-17 | 2021-08-17 | Consejo Superior Investigacion | BIOCONJUGATES AND ANTIBODIES FOR THE DERIVATIZATION-ASSISTED IMMUNODETECTION OF PATULIN MYCOTOXIN (Machine-translation by Google Translate, not legally binding) |
-
2012
- 2012-06-15 CN CN 201220291285 patent/CN202710570U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109085159A (en) * | 2017-08-21 | 2018-12-25 | 上海荒岛科技有限公司 | A kind of substrate and detection card |
| CN109085348A (en) * | 2017-08-21 | 2018-12-25 | 上海荒岛科技有限公司 | A kind of method for analyzing body fluid |
| CN109085348B (en) * | 2017-08-21 | 2019-07-23 | 上海荒岛科技有限公司 | A kind of method for analyzing body fluid |
| ES2849398A1 (en) * | 2020-02-17 | 2021-08-17 | Consejo Superior Investigacion | BIOCONJUGATES AND ANTIBODIES FOR THE DERIVATIZATION-ASSISTED IMMUNODETECTION OF PATULIN MYCOTOXIN (Machine-translation by Google Translate, not legally binding) |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130130 Termination date: 20200615 |