CN202710559U - Kit for quick detection of odontoglossum ringspot viruses - Google Patents
Kit for quick detection of odontoglossum ringspot viruses Download PDFInfo
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- CN202710559U CN202710559U CN 201220287748 CN201220287748U CN202710559U CN 202710559 U CN202710559 U CN 202710559U CN 201220287748 CN201220287748 CN 201220287748 CN 201220287748 U CN201220287748 U CN 201220287748U CN 202710559 U CN202710559 U CN 202710559U
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- odontoglossum
- ring spot
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Abstract
The utility model provides a kit for quick detection of odontoglossum ringspot viruses, and belongs to biological detection consumables. The kit for the quick detection of the odontoglossum ringspot viruses is characterized in that a detection item mark is arranged between a sample adding hole and a result observation hole, a pair of opposite angles on the upper surface of a box sealed body and the lower surface of the box sealed body are arc-shaped, air holes are further arranged in the box sealed body. A gold mark fiber cushion bag of a colloidal gold test strip is coated with odontoglossum ringspot virus monoclonal antibodies, a detection line T line and a control line C line of a cellulose sensitive film of the colloidal gold test strip are respectively coated with the odontoglossum ringspot virus monoclonal antibodies and rabbit antibody mouse intravenous gamma globulin polyclonal antibodies. According to the kit for the quick detection of the odontoglossum ringspot viruses, the principle of a double antibody sandwich method and colloidal gold immunoassay are adopted by the test strip to be used for detection of various orchid odontoglossum ringspot viruses. The kit for the quick detection of the odontoglossum ringspot viruses is simple and direct, quick, sensitive, accurate, special, easy to store, convenient to use, attractive and elegant.
Description
Technical field
The utility model relates to field of biological detection, particularly relates to a kind of kit of fast detecting odontoglossum ring spot virus.
Background technology
Odontoglossum ring spot virus (Odontoglossum ringspot virus, ORSV), Tobamovirus is one of two kinds of main viruses of harm orchid, distributes all over the world main harm tooth orchid, sword-leaved cymbidium etc.In a single day orchid infects ORSV, and its blade factory produces yellow striped or the irregular Green Patch that takes off, diseased plant flower section even lopsided or the spot that fades occur, and the red colour system flower spot that fades is particularly evident, the ornamental value of orchid and economic worth is caused have a strong impact on.Studies show that this virus is quite general for various orchids.
At present, the detection method of odontoglossum ring spot virus is commonly used enzyme linked immunosorbent assay (ELISA) (ELISA) method, EIA enzyme immunoassay is the technology that the specificity with the antigen-antibody combination combines with the efficient catalytic of enzyme, have highly sensitive, the characteristics such as high specificity, but operation steps is many, detects forward and backward all need to do necessary instrument cleaning and maintenance work, detection time is long, and requires this area professional and technical personnel to operate.
Immune colloidal gold technique (Immunogold labelling techique) is the solid phase labelling immunoassay that grows up after fluorescein, radioactive isotope and enzyme three large labelling techniques the eighties in last century.This technology has mainly utilized gold grain to have the characteristic of high electron density, when these labels are assembled in a large number at corresponding part place, forms macroscopic redness or pink spot, thereby is used for qualitative or semiquantitative tachysynthesis detection method.Fast diagnose test paper bar is a novel vitro diagnostic techniques that has grown up on monoclonal antibody technique, colloidal gold immunochromatographimethod technology and novel chromatographic material basis since the nineties in 20th century, development in recent years is rapid, particularly is widely applied in the medical test at biomedical sector.This technology mainly is that specific antigen or antibody are fixed on the nitrocellulose membrane with ribbon, colloid gold label reagent is adsorbed on the pad, be added on the sample pad of test strips one end when testing sample after, move forward by capillary action, react to each other behind the colloid gold label reagent on the dissolving pad, when moving to again fixing antigen or antibody regions, specific binding occurs with it and is trapped in determinand gold marked reagent compound, colloid gold label is gathered in to detect and is with, can be by the range estimation result that developed the color intuitively.Free label is then crossed and is detected band, thereby reaches the purpose of automatically separating with the detection thing.
At present, the double antibodies sandwich method combined with the collaurum detection technique odontoglossum ring spot virus is carried out fast detecting, and have efficiently, sensitive, special, convenient, the colloidal gold kit of economic dispatch characteristics rarely has report.
The utility model content
Be demand and the blank that solves above-mentioned field, the utility model aims to provide a kind of kit of fast detecting odontoglossum ring spot virus, be used for the detection of orchid class odontoglossum ring spot virus, the utility model kit adopts double antibody sandwich method principle and the immune colloidal gold technique fast detecting odontoglossum ring spot virus that combines.
A kind of kit of fast detecting odontoglossum ring spot virus comprises the box obturator that box body, lid consist of and places colloidal gold strip in the box body, has well, test item sign and viewport as a result on the described lid; It is characterized in that: described test item sign is located at described well and described as a result between the viewport, the a pair of diagonal angle of described box obturator upper and lower surface is arc, also be provided with bleeder vent on the described box obturator, the gold mark fiber mat of described colloidal gold strip is coated with the odontoglossum ring spot virus monoclonal antibody of colloid gold label, is coated with respectively odontoglossum ring spot virus monoclonal antibody and the anti-mouse IgG of rabbit on the detection line T line on the cellulose medicine film of described colloidal gold strip and the control line C line and resists more.
Described bleeder vent is located at described box obturator lid one side.
Described bleeder vent is a plurality of, is fan-shaped array.
Being provided with the locating slot that colloidal gold strip is installed in the described box body, when described colloidal gold strip is positioned over locating slot in the box body, is the sample fiber pad corresponding to lid well place; Being gold mark fiber mat corresponding to test item sign place, is cellulose medicine film corresponding to described as a result viewport place.
Described box body is provided with coupling arrangement, and described lid is connected the detachable connection of coupling arrangement with box body.
One side corresponding to box body on the described lid is provided with a plurality of apertures, and described box body is provided with projection with described box body one corresponding to the one side of lid, and the aperture population size on described projection and the described lid is suitable.
Described box body is provided with sample number into spectrum.
Described sample number into spectrum is located at described box obturator lid one side, and a side of close described as a result viewport.
Technique effect:
The kit of fast detecting odontoglossum ring spot virus of the present utility model comprises the box obturator that box body, lid consist of and places colloidal gold strip in the box body, has well, test item sign and viewport as a result on the described lid; It is characterized in that: described test item sign is located at described well and described as a result between the viewport, be convenient to observe and use, be convenient to identify concrete test item, the a pair of diagonal angle of described box obturator upper and lower surface is arc, save material space, and elegant in appearance, also be provided with bleeder vent on the described box obturator, so that can keep air-flow to communicate with the external world in the box body, when detecting, guarantee that reaction film normally brings into play capillarity, make detect thing can be fully rapidly displacement to ventilative nose end, avoid the phenomenons such as retardance or incomplete swimming, guaranteed the sensitivity that detects, accuracy and high-level efficiency.The odontoglossum ring spot virus monoclonal antibody that the gold mark fiber mat of described colloidal gold strip is coated with colloid gold label is used in conjunction with odontoglossum ring spot virus, be coated with respectively the odontoglossum ring spot virus monoclonal antibody on set detection line T line and the control line C line on the cellulose medicine film of described colloidal gold strip and how anti-the anti-mouse IgG of rabbit is, be used for the colour developing of concrete specific reaction.
The utility model is preferably opened bleeder vent on described lid.Preventing from detecting liquid out dyes on operating personnel's the skin from the bleeder vent water clock.
The further preferred bleeder vent of the utility model is a plurality of apertures, is fan-shaped array.The design of a plurality of apertures makes air hole both play the effect of ventilation, can not allow again reaction film come out, thereby avoid polluteing Person's skin.Fan-shaped array is comparatively attractive in appearance.
Be provided with the locating slot that colloidal gold strip is installed in the box body described in the utility model, when described colloidal gold strip is positioned over locating slot in the box body, be the sample fiber pad corresponding to lid well place, being gold mark fiber mat corresponding to test item sign place, is cellulose medicine film corresponding to described as a result viewport place.
Described box obturator is provided with coupling arrangement, and described lid is connected the detachable connection of coupling arrangement with box body, on the one hand convenient use, and box body and lid are reusable on the other hand, saving resource.
One side corresponding to box body on the described lid is provided with a plurality of apertures, described box body is provided with projection with described box body one corresponding to the one side of lid, and the aperture population size on described projection and the described lid is suitable, and processing and fabricating is simple, for convenience detach, be difficult for polluting.
Also be provided with sample number into spectrum on the described box obturator, be convenient to search, distinguish, be convenient to identify product category and test event.
The preferred described sample number into spectrum of the utility model is located at described box obturator lid one side, and a side of close described as a result viewport, easy to use and observation.
The utility model is simple to operate, easy to use, bring i.e. usefulness, do not need one of skill in the art, without any need for instrument, be applicable to the needs of odontoglossum ring spot virus fast detecting in all kinds of orchids, can satisfy different levels personnel's needs, popularize easily, show the result with symbol, break away from the specific (special) requirements to equipment, instrument, personnel and storage and transportation environment, and had quick, easy, sensitive, accurate, special characteristics.
Description of drawings
Fig. 1 the utility model kit lid synoptic diagram
Fig. 2 the utility model kit preferred version lid synoptic diagram
Fig. 3 the utility model kit synoptic diagram
Fig. 4 the utility model kit preferred version synoptic diagram
Fig. 5 the utility model kit box body synoptic diagram
Fig. 6 the utility model colloidal gold strip synoptic diagram
1-lid wherein, the 2-box body, the 3-well, 4-test item sign, 5-is viewport as a result, 6-box obturator, 7 sample number into spectrum, 8-colloidal gold strip, 9-projection, 10-sample fiber pad, 11-gold mark fiber mat, 12-cellulose medicine film, 13-water-absorption material backing strap, 14-bleeder vent.
Embodiment:
It is in order further to understand better the utility model that following embodiment is provided; be not limited to described preferred forms; content of the present utility model and protection domain are not construed as limiting; anyone makes up with the feature of other prior aries under enlightenment of the present utility model or with the utility model and the identical or akin product of any and the utility model that draws, all drops within the protection domain of the present utility model.
The utility model is simple to operate, easy to use, and is elegant in appearance, with low cost, has quick, accurate, convenient, inexpensive characteristics.
ORSV in the Figure of description refers to that the odontoglossum ring spot virus test indicates item.
The kit of fast detecting odontoglossum ring spot virus of the present utility model comprises the box obturator 6 that box body 2, lid 1 consists of and places colloidal gold strip 8 in the box body 2, has well 3, test item sign 4 and viewport 5 as a result on the described lid 1; It is characterized in that: described test item sign 4 is located between described well 3 and the described as a result viewport 5, be convenient to observe and use, be convenient to identify concrete test item, the a pair of diagonal angle of described box obturator 6 upper and lower surfaces is arc, save material space, and elegant in appearance, also be provided with bleeder vent 14 on the described box obturator 6, so that can keep air-flow to communicate with the external world in the box body 2, when detecting, guarantee that reaction film normally brings into play capillarity, make detect thing can be fully rapidly displacement to bleeder vent 14 ends, avoid the phenomenons such as retardance or incomplete swimming, guaranteed the sensitivity that detects, accuracy and high-level efficiency.The odontoglossum ring spot virus monoclonal antibody that the gold mark fiber mat 11 of described colloidal gold strip 8 is coated with colloid gold label is used in conjunction with odontoglossum ring spot virus, be coated with respectively the odontoglossum ring spot virus monoclonal antibody on set detection line T line and the control line C line on the cellulose medicine film 12 of described colloidal gold strip 8 and how anti-the anti-mouse IgG of rabbit is, be used for the colour developing of concrete specific reaction.
The utility model is preferably opened bleeder vent 14 on described lid 1.Control detects liquid and out dyes on operating personnel's the skin from bleeder vent 14 water clocks.
The further preferred bleeder vent 14 of the utility model is a plurality of apertures, is fan-shaped array.The design of a plurality of apertures makes air hole both play the effect of ventilation, can not allow again reaction film come out, thereby avoid polluteing Person's skin.Fan-shaped array is comparatively attractive in appearance.
Be provided with the locating slot that colloidal gold strip 8 is installed in the box body 2 described in the utility model, when described colloidal gold strip 8 is positioned over locating slot in the box body 2,3 places are the sample fiber pad corresponding to lid 1 well, identifying 4 places for gold mark fiber mat 11 corresponding to test item, is cellulose medicine film 12 corresponding to described as a result viewport 5 places.
Described box obturator 6 is provided with coupling arrangement, and described lid 1 is connected with box body by the detachable connection of coupling arrangement, on the one hand convenient use, and box body 2 and lid 1 are reusable on the other hand, saving resource.
One side corresponding to box body 2 on the described lid 1 is provided with a plurality of apertures, described box body 2 is provided with projection with described box body 2 one corresponding to the one side of lid 1, and the aperture population size on described projection and the described lid 1 is suitable, and processing and fabricating is simple, for convenience detach, be difficult for polluting.
Also be provided with sample number into spectrum 7 on the described box obturator 6, be convenient to search, distinguish, be convenient to identify product category and test event.
The preferred described sample number into spectrum 7 of the utility model is located at described box obturator 6 lids 1 one side, and a side of close described as a result viewport 5, easy to use and observation.
The preparation method of experimental example 2. the utility model test strips
The preparation procedure of test strips is as follows
Being coated with of step 1. cellulose medicine film
1, with the anti-mouse IgG of rabbit anti-in contrast line C lines how, the odontoglossum ring spot virus monoclonal antibody is as detection line T line, and two reagent are respectively according to required concentration (scope 1.5-2mg/ml), dilutes with the PBS of 20mM.
2, with the mentioned reagent for preparing, coated to nitrocellulose filter respectively according to C, T line position, then dry 6 hours, temperature was 30 ℃, humidity≤35%, thus make cellulose medicine film.
The fibrolaminar mark of step 2. gold mark:
1, gets the colloidal gold solution of 40nm, press final concentration 8-12 μ l/ml and add 0.2M K
2CO
3, add certain density odontoglossum ring spot virus monoclonal antibody, placed room temperature reaction 15-30 minute, then add the solution of 20%BSA according to concentration 20 μ l/ml, be used for saturated free collaurum.Last 10000rpm centrifugal 30 minutes, removes unconjugated protein in the supernatant.Colloid gold label stoste with isopyknic 0.5m BAS (containing 1%Tween, 5% sucrose, 1% hydrolysis BSA, 1% caseinhydrolysate, 0.1%PVA) redissolution precipitation.
2, with above-mentioned colloid gold label stoste according to 10-20% concentration, dilute with the 0.5m BAS, thereby be mixed with collaurum working fluid behind the mark.And then this working fluid is laid on the 30*30cm fiberglass packing, dry 6 hours, temperature was 30 ℃, humidity≤35%, thus make gold mark pad.
The assembling of step 3. test strips:
1, plastic plate is lain on the operator's console, stick double sticky tape.
2, tear the double sticky tape lining paper, the nitrocellulose filter that is coated with odontoglossum ring spot virus monoclonal antibody and the anti-mouse IgG polyclonal antibody of rabbit is sticked at the 30mm place in distance plastic plate upper end.
3, robust fibre is absorbed water letterweight nitrocellulose filter upper end 2mm sticks, and evens up on upper end and the plastic plate.
4, press 0.5mm place, nitrocellulose filter lower end to stick little nonwoven fabrics.
5, gold mark pad is evened up be pressed on above the little nonwoven fabrics.
6, fiberglass packing is pressed on gold mark pad upper approximately half, stick apart from 2mm place, plastic plate lower end the lower end.
7, large nonwoven fabrics and gold mark pad upper end are evened up, lower end and plastic plate lower end are evened up and are sticked.
8, press nitrocellulose filter 1.5-2mm to stick on the arrow adhesive tape.
9, press robust fibre thieving paper to stick on the handheld terminal adhesive tape.
The kit of a kind of fast detecting odontoglossum ring spot virus of the utility model adopts combine odontoglossum ring spot virus in all kinds of orchids of fast detecting of double antibodies sandwich method principle and immune colloidal gold technique.The utility model test strips is simple to operate, easy to use, bring namely and use, do not need one of skill in the art, can satisfy different levels personnel's needs, popularize easily, the utility model shows the result with symbol, break away from the specific (special) requirements to equipment, instrument, personnel and storage and transportation environment, be used for the detection of orchid class odontoglossum ring spot virus.The utility model kit is simple and direct fast sensitive and accurate, and preserves easily, and is easy to use, elegant in appearance.
Claims (6)
1. the kit of a fast detecting odontoglossum ring spot virus comprises the box obturator that box body, lid consist of and places colloidal gold strip in the box body, has well, test item sign and viewport as a result on the described lid; It is characterized in that: described test item sign is located at described well and described as a result between the viewport, the a pair of diagonal angle of described box obturator upper and lower surface is arc, also be provided with bleeder vent on the described box obturator, the gold mark fiber mat of described colloidal gold strip is coated with the odontoglossum ring spot virus monoclonal antibody of colloid gold label, is coated with respectively odontoglossum ring spot virus monoclonal antibody and the anti-mouse IgG of rabbit on the detection line T line on the cellulose medicine film of described colloidal gold strip and the control line C line and resists more.
2. the kit of fast detecting odontoglossum ring spot virus according to claim 1 is characterized in that, described bleeder vent is located at described box obturator lid one side.
3. the kit of fast detecting odontoglossum ring spot virus according to claim 2 is characterized in that, described bleeder vent is a plurality of, is fan-shaped array.
4. the kit of fast detecting odontoglossum ring spot virus according to claim 3, it is characterized in that, being provided with the locating slot that colloidal gold strip is installed in the described box body, when described colloidal gold strip is positioned over locating slot in the box body, is the sample fiber pad corresponding to lid well place; Be gold mark fiber mat corresponding to test item sign place; Be cellulose medicine film corresponding to described as a result viewport place.
5. the kit of fast detecting odontoglossum ring spot virus according to claim 4 is characterized in that, described box body is provided with coupling arrangement, and described lid is connected the detachable connection of coupling arrangement with box body.
6. the kit of fast detecting odontoglossum ring spot virus according to claim 5, it is characterized in that, one side corresponding to box body on the described lid is provided with a plurality of apertures, described box body is provided with projection with described box body one corresponding to the one side of lid, and the aperture population size on described projection and the described lid is suitable.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220287748 CN202710559U (en) | 2012-06-15 | 2012-06-15 | Kit for quick detection of odontoglossum ringspot viruses |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220287748 CN202710559U (en) | 2012-06-15 | 2012-06-15 | Kit for quick detection of odontoglossum ringspot viruses |
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| Publication Number | Publication Date |
|---|---|
| CN202710559U true CN202710559U (en) | 2013-01-30 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220287748 Expired - Fee Related CN202710559U (en) | 2012-06-15 | 2012-06-15 | Kit for quick detection of odontoglossum ringspot viruses |
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| CN (1) | CN202710559U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104122389A (en) * | 2014-04-14 | 2014-10-29 | 中国科学院寒区旱区环境与工程研究所 | Reagent card for colloidal gold immunochromatograohic assay of lily mottle virus and preparation method of reagent card for colloidal gold immunochromatograohic assay of lily mottle virus |
| CN107860920A (en) * | 2017-11-01 | 2018-03-30 | 杭州微瑞科技有限公司 | Rabies virus antibodies Quantitative detection card and application method |
| CN107860921A (en) * | 2017-11-01 | 2018-03-30 | 杭州微瑞科技有限公司 | Pseudorabies virus antibody Quantitative detection card and application method |
-
2012
- 2012-06-15 CN CN 201220287748 patent/CN202710559U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104122389A (en) * | 2014-04-14 | 2014-10-29 | 中国科学院寒区旱区环境与工程研究所 | Reagent card for colloidal gold immunochromatograohic assay of lily mottle virus and preparation method of reagent card for colloidal gold immunochromatograohic assay of lily mottle virus |
| CN104122389B (en) * | 2014-04-14 | 2015-03-11 | 中国科学院寒区旱区环境与工程研究所 | Reagent card for colloidal gold immunochromatograohic assay of lily mottle virus and preparation method of reagent card for colloidal gold immunochromatograohic assay of lily mottle virus |
| CN107860920A (en) * | 2017-11-01 | 2018-03-30 | 杭州微瑞科技有限公司 | Rabies virus antibodies Quantitative detection card and application method |
| CN107860921A (en) * | 2017-11-01 | 2018-03-30 | 杭州微瑞科技有限公司 | Pseudorabies virus antibody Quantitative detection card and application method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130130 Termination date: 20200615 |