CN207067142U - Colloidal gold strip and detection means - Google Patents
Colloidal gold strip and detection means Download PDFInfo
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- CN207067142U CN207067142U CN201720980726.5U CN201720980726U CN207067142U CN 207067142 U CN207067142 U CN 207067142U CN 201720980726 U CN201720980726 U CN 201720980726U CN 207067142 U CN207067142 U CN 207067142U
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Abstract
The utility model provides a kind of colloidal gold strip and detection means, belong to technical field of immunological detection, the colloidal gold strip includes sample pad, colloidal gold pad, NC films and the adsorptive pads that bottom plate, fixture and Autonomous test initial end to detection terminal are set gradually；Covered with fixture on sample pad, colloidal gold pad and part NC films, using the colloidal gold strip of said structure, with good reinforcing and crown_interception, using fixture colloidal gold pad, NC films and sample pad location can be made more firm, play the effect of damming simultaneously, the homogeneity and antigen and the adequacy of gold labeling antibody reaction of collaurum release can be improved, so as to improve the sensitivity of collaurum product, shorten the exhibition layer time of collaurum and improve exhibition layer effect, prevent and rush sample, chromatography not exclusively causes experimental result inaccuracy situation.Detection means provided by the utility model, including above-mentioned colloidal gold strip, there is the characteristics of colloidal gold strip.
Description
Technical field
It the utility model is related to technical field of immunological detection, and in particular to a kind of colloidal gold strip and detection means.
Background technology
Colloidal gold strip grown up since being the 1990s on colloidal gold immunochromatographimethod material foundation one
Item novel in vitro diagnostic techniques, quickly grows in recent years, extensively should have been obtained in biomedical sector particularly medical test
With.Specific antigen or antibody are mainly fixed on NC films (nitrocellulose filter) by the technology with ribbon, collaurum
Labelled reagent adsorb in colloidal gold pad, after testing sample is added in the sample pad of test strips one end, by capillarity to
Preceding movement, reacted to each other after dissolving the colloid gold label reagent in colloidal gold pad, then be moved to fixed antigen or antibody regions
When, determinand gold marked reagent compound occurs to specifically bind and be trapped therewith, and colloid gold label is gathered in detection band, can
By estimating the result that intuitively developed the color.And free label then exceedes detection band, reach from face and divide automatically with detectable substance
From purpose.
Existing colloidal gold pad, NC films, sample pad etc. are typically to be pasted onto on bottom plate, especially for colloidal gold pad, chi
It is very little smaller, easily shift, detection is affected greatly；It is in addition, not any between colloidal gold pad, NC films and sample pad
Structure is retained, user is when in use, it is necessary in strict accordance with dropwise addition blood flow volume is required, once exceeding dripping quantity, blood can directly be run
Onto NC films, testing result is influenceed.
Utility model content
First purpose of the present utility model is to provide a kind of colloidal gold strip that there is good reinforcing and retention to make
With, using fixture colloidal gold pad, NC films and sample pad location can be made more firm, while the effect of damming is played, it can improve
The homogeneity and antigen and the adequacy of gold labeling antibody reaction of collaurum release, so as to improve the sensitivity of collaurum product,
Shorten the exhibition layer time of collaurum and improve exhibition layer effect, prevent and rush sample, chromatography not exclusively causes experimental result inaccurate
Situation.
Second purpose of the present utility model is to provide a kind of detection means, and the detection means includes colloidal gold strip.
Based on above-mentioned first purpose, colloidal gold strip provided by the utility model, including；Bottom plate, sample pad, collaurum
Pad, NC films, adsorptive pads and fixture；
The bottom plate has a detection initial end and detection terminal, from the detection initial end to the detection terminal successively
The sample pad, the colloidal gold pad, the NC films and the adsorptive pads are set；
Wherein, the sample pad is partially covered in the colloidal gold pad, and the colloidal gold pad is partially covered on the NC
One end of film, the adsorptive pads are partially covered on the other end of the NC films；
Covered with fixture on the sample pad, the colloidal gold pad and part NC films, so that the sample pad, described
Colloidal gold pad and part NC film relative position firms, and the effect of damming can be played.
Further, the NC films middle part detection line is coated with antigen or antibody.
Further, the antigen or antibody of gold mark are coated with described colloidal gold pad.
Further, the sample pad, colloidal gold pad, NC films and the adsorptive pads are connected with the base plate sticking respectively.
Further, in addition to hemofiltration pad, it is arranged between the sample pad and the colloidal gold pad, wherein, it is described
Sample pad is partially covered on the hemofiltration pad, and the hemofiltration pad part is covered in the colloidal gold pad.
Further, covered with fixture on sample segment pad, hemofiltration pad, colloidal gold pad and part NC films.
Further, the fixing device uses adhesive tape.
Further, the colloidal gold pad is using the glass fibre or polyester for coating gold labeling antibody.
Further, the adsorptive pads use absorbent filter.
Based on above-mentioned second purpose, a kind of detection means provided by the utility model, including collaurum as described above examination
Paper slip.
The beneficial effect of colloidal gold strip provided by the utility model：
Colloidal gold strip provided by the utility model, including bottom plate, sample pad, colloidal gold pad, NC films, adsorptive pads and solid
Determine part；There is bottom plate a detection initial end and detection terminal, Autonomous test initial end to detection terminal to set gradually sample pad, colloid
Gold pad, NC films and adsorptive pads；
Wherein, sample pad is partially covered in colloidal gold pad, and colloidal gold pad is partially covered on one end of NC films, adsorptive pads portion
Divide the other end for being covered in NC films；Covered with fixture on sample pad, colloidal gold pad and part NC films, so that sample pad, glue
Body gold pad and part NC film relative position firms.
Using the colloidal gold strip of said structure, there is good reinforcing and crown_interception, can be made using fixture
Colloidal gold pad, NC films and sample pad location are more firm, while play the effect of damming, can improve collaurum release homogeneity with
And antigen and the adequacy of gold labeling antibody reaction, so as to improve the sensitivity of collaurum product, shorten the exhibition layer time of collaurum
Layer effect is opened up with improving, prevents and rushes sample, chromatography not exclusively causes experimental result inaccuracy situation.
Detection means provided by the utility model, including above-mentioned colloidal gold strip, there is the upper of colloidal gold strip
State advantage.
Brief description of the drawings
, below will be right in order to illustrate more clearly of the utility model embodiment or technical scheme of the prior art
The required accompanying drawing used is briefly described in embodiment or description of the prior art, it should be apparent that, describe below
In accompanying drawing be some embodiments of the present utility model, for those of ordinary skill in the art, do not paying creativeness
On the premise of work, other accompanying drawings can also be obtained according to these accompanying drawings.
Fig. 1 is the structural representation for the colloidal gold strip that the utility model embodiment one provides；
Fig. 2 is Fig. 1 top view；
Fig. 3 is the structure for the colloidal gold strip for micro whole blood pattern detection that the utility model embodiment two provides
Schematic diagram；
Fig. 4 is the structure for the colloidal gold strip for being used for the detection of a large amount of whole blood samples that the utility model embodiment three provides
Schematic diagram.
Icon：100- bottom plates；110- detects initial end；120- detection terminals；200- sample pads；300- colloidal gold pads；
400-NC films；500- adsorptive pads；600- fixtures；700- hemofiltration pads.
Embodiment
The technical solution of the utility model is clearly and completely described below in conjunction with accompanying drawing, it is clear that described
Embodiment is the utility model part of the embodiment, rather than whole embodiments.Based on the embodiment in the utility model, sheet
The every other embodiment that field those of ordinary skill is obtained under the premise of creative work is not made, belongs to this practicality
Novel protected scope.
, it is necessary to explanation in description of the present utility model, term " " center ", " on ", " under ", it is "left", "right", " perpendicular
Directly ", the orientation of the instruction such as " level ", " interior ", " outer " or position relationship are based on orientation shown in the drawings or position relationship, are only
Described for the ease of description the utility model and simplifying, rather than instruction or imply signified device or element must have it is specific
Orientation, with specific azimuth configuration and operation, therefore it is not intended that to limitation of the present utility model.In addition, term " the
One ", " second ", " the 3rd " are only used for describing purpose, and it is not intended that instruction or hint relative importance.
, it is necessary to which explanation, unless otherwise clearly defined and limited, term " are pacified in description of the present utility model
Dress ", " connected ", " connection " should be interpreted broadly, for example, it may be fixedly connected or be detachably connected, or integratedly
Connection；Can be mechanical connection or electrical connection；Can be joined directly together, can also be indirectly connected by intermediary,
It can be the connection of two element internals.For the ordinary skill in the art, above-mentioned art can be understood with concrete condition
Concrete meaning of the language in the utility model.
Embodiment one
It refer to shown in Fig. 1 and Fig. 2；A kind of colloidal gold strip is present embodiments provided, the colloidal gold strip has
Good reinforcing and crown_interception, colloidal gold pad 300, NC films 400 and the position of sample pad 200 can be made more using fixture 600
It is firm, while the effect of damming is played, the homogeneity and antigen that can improve collaurum release react abundant with gold labeling antibody
Property, so as to improve the sensitivity of collaurum product, shorten the exhibition layer time of collaurum and improve exhibition layer effect, prevent punching
Sample, chromatography not exclusively cause experimental result inaccuracy situation.
It refer to shown in Fig. 1 and Fig. 2；The colloidal gold strip includes；Bottom plate 100, sample pad 200, colloidal gold pad 300,
NC films 400, adsorptive pads 500 and fixture 600.
The bottom plate 100 preferably uses PVC material, has a detection initial end 110 and detection terminal 120 on bottom plate 100
(respectively positioned at the both sides of the length direction of bottom plate 100), Autonomous test initial end 110 to detection terminal 120 set gradually sample pad
200th, colloidal gold pad 300, NC films 400 and adsorptive pads 500；
Wherein, sample pad 200 is partially covered in colloidal gold pad 300, and colloidal gold pad 300 is partially covered on NC films 400
One end (close to the position of detection initial end 110), adsorptive pads 500 are partially covered on the other end of NC films 400 (close to detection terminal
120 position)；
It is preferred covered with fixture 600, the fixture 600 on sample pad 200, colloidal gold pad 300 and part NC films 400
Using adhesive tape.On the one hand the effect of fixture 600 is to make sample pad 200, colloidal gold pad 300 and the phase of part NC films 400
It is more firm to position, prevent that position shifts when sample pad 200, colloidal gold pad 300 or part NC films 400 from using, and causes
Detection failure；Another effect of fixture 600 is to play the effect of damming, and fixture 600 only needs to cover 1- on NC films 400
2mm is wide, and other parts are then pasted onto in colloidal gold pad 300 and sample pad 200, can suitably increase and be arranged on sample pad
The width of 200 upper fixing elements 600, such arrangement can prevent having when user from using not according to requirement dropwise addition blood volume etc. is added dropwise
Even more than require doubling dose, when such, damming effect is played using the fixture 600 of adhesive tape, effectively prevent
Hemostatic Oral Liquid moves directly to NC films 400, prevents and rushes sample, chromatography not exclusively causes experimental result inaccuracy situation.
Preferably, the colloidal gold pad 300 is using the glass fibre or polyester for coating gold labeling antibody.
Preferably, the adsorptive pads 500 use absorbent filter.
Preferably, sample pad 200, colloidal gold pad 300, NC films 400, adsorptive pads 500 are respectively adhered on bottom plate 100.
Embodiment two
Present embodiments provide a kind of colloidal gold strip for being used for (5-20 microlitres) detection of micro whole blood sample, the implementation
The colloidal gold strip for the micro whole blood pattern detection that example provides is in a kind of colloidal gold strip of offer of above-described embodiment
The improvement made on architecture basics, it is specific as follows：
Refer to shown in Fig. 3, the embodiment be used for micro whole blood pattern detection colloidal gold strip include bottom plate 100,
Sample pad 200, colloidal gold pad 300, NC films 400, hemofiltration pad 700, adsorptive pads 500 and fixture 600.
Wherein, there is a detection initial end 110 and detection terminal 120 (respectively positioned at the length side of bottom plate 100 on bottom plate 100
To both sides), Autonomous test initial end 110 to detection terminal 120 sets gradually sample pad 200, hemofiltration pad 700, colloidal gold pad
300th, NC films 400 and adsorptive pads 500；On the part of sample pad 200 covering hemofiltration pad 700, hemofiltration pad 700 is partially covered on collaurum
On pad 300, colloidal gold pad 300 is partially covered on one end (close to the position of detection initial end 110) of NC films 400, adsorptive pads 500
It is partially covered on the other end of NC films 400 (close to the position of detection terminal 120)；
Fixture 600 is covered on sample pad 200, hemofiltration pad 700, colloidal gold pad 300 and part NC films 400.It is fixed
On the one hand the effect of part 600 is to make sample pad 200, colloidal gold pad 300, hemofiltration pad 700 and the relative position of part NC films 400 more
It is firm, prevent that position shifts when sample pad 200, colloidal gold pad 300, hemofiltration pad 700 or part NC films 400 from using, and makes
Failed into detection；Another effect of fixture 600 is to play the effect of damming, and fixture 600 only needs to cover 1- on NC films 400
2mm is wide, and other parts are then pasted onto in colloidal gold pad 300, hemofiltration pad 700 and sample pad 200, can suitably increase setting
In the width of the upper fixing element 600 of sample pad 200, such arrangement can prevent from not requiring dropwise addition blood according to dropwise addition when user from using
Amount etc., some are even more than desired doubling dose, when such, the effect of damming are played using the fixture 600 of adhesive tape,
Effective anti-Hemostatic Oral Liquid moves directly to NC films 400, prevents and rushes sample, chromatography not exclusively causes experimental result inaccuracy situation.
In the embodiment, it is preferable that absorbent filter length is arranged to 31mm or so, and the length of bottom plate 100 is arranged to a 80mm left sides
The right side, the length of NC films 400 are arranged on 25mm or so, and the length of colloidal gold pad 300 is arranged on 6mm or so, adhesive tape (fixture 600)
Length is arranged on 15mm or so, and the length of sample pad 200 is arranged on 16mm or so.
The colloidal gold strip that the present embodiment provides can effectively stop the red blood cell in micro whole blood sample, prevent red thin
Born of the same parents enter reaction system, influence experimental result, antigen is combined with colloidal gold labeled monoclonal antibody more abundant, sensitivity is higher.
Embodiment three
It refer to shown in Fig. 4, present embodiments provide a kind of glue for being used for (50-100 microlitres) detection of a large amount of whole blood samples
Body gold test paper strip, it is to use above-described embodiment one that what the embodiment provided, which is used for the colloidal gold strip that a large amount of whole blood samples detect,
The structure for the colloidal gold strip that kind provides, sample pad 200 is specifically arranged via the hemofiltration pad 700 after processing.This reality
Apply in an accompanying drawing, be hemofiltration pad 700 by the Direct Mark of sample pad 200 for convenience of description.
When it is implemented, Autonomous test initial end 110 to detection terminal 120 sets gradually hemofiltration pad 700, glue on bottom plate 100
Body gold pad 300, NC films 400 and adsorptive pads 500；
Wherein, hemofiltration pad 700 is partially covered in colloidal gold pad 300, and colloidal gold pad 300 is partially covered on NC films 400
One end (close to the position of detection initial end 110), adsorptive pads 500 are partially covered on the other end of NC films 400 (close to detection terminal
120 position)；
Covered with fixture 600, the effect of fixture 600 on hemofiltration pad 700, colloidal gold pad 300 and part NC films 400
On the one hand it is to make hemofiltration pad 700, colloidal gold pad 300 and part 400 relative positions of NC films more firm；Fixture 600 it is another
Effect is to play the effect of damming, and fixture 600 only needs to cover on NC films 400 that 1-2mm is wide, and other parts are then pasted onto
In colloidal gold pad 300 and hemofiltration pad 700, it can suitably increase and be arranged on the width that hemofiltration pads 700 upper fixing elements 600, it is such
Arrangement can prevent that some is even more than desired doubling dose, so not according to requirement dropwise addition blood volume etc. is added dropwise when user from using
When, the effect of damming is played using the fixture 600 of adhesive tape, effective anti-Hemostatic Oral Liquid moves directly to NC films 400, prevents out
Now rush sample, chromatography not exclusively causes experimental result inaccuracy situation.
Preferably, the sample pad 200, colloidal gold pad 300, NC films 400 and the adsorptive pads 500 respectively with the bottom plate
100 paste connection.
In the embodiment, it is preferable that absorbent filter length is arranged to 31mm or so, and the length of bottom plate 100 is arranged to a 80mm left sides
The right side, the length of NC films 400 are arranged on 25mm or so, and the length of colloidal gold pad 300 is arranged on 6mm or so, adhesive tape (fixture 600)
Length is arranged on 11mm or so, and hemofiltration pads 700 length and is arranged on 21mm or so.
What the present embodiment provided, which is used for the colloidal gold strip that a large amount of whole blood samples detect, can effectively stop a large amount of whole bloods
Red blood cell in sample, prevent red blood cell from entering reaction system, influence experimental result, antigen is combined with colloidal gold labeled monoclonal antibody
More fully, sensitivity is higher.
Example IV
It refer to shown in Fig. 1 and Fig. 2, present embodiments provide a kind of for detecting cTnI colloidal gold strips, the implementation
It is to use a kind of structure of the colloidal gold strip of offer of above-described embodiment that what example provided, which is used to detect cTnI colloidal gold strips,
The middle part detection line of NC films 400 is specifically coated with anti-cTnI monoclonal antibodies or polyclonal antibody.In colloidal gold pad 300
On be coated with gold mark anti-cTnI antibody.
In the embodiment, it is preferable that absorbent filter length is arranged to 31mm or so, and the length of bottom plate 100 is arranged to a 80mm left sides
The right side, the length of NC films 400 are arranged on 25mm or so, and the length of colloidal gold pad 300 is arranged on 6mm or so, adhesive tape (fixture 600)
Length is arranged on 11mm or so, and the length of sample pad 200 is arranged on 21mm or so.
It is provided by the utility model to be used to detect cTnI colloidal gold strips, early prediction can be carried out to myocardial infarction,
With the features such as quick, simple to operate, it is suitable for bedside quick diagnosis.
Embodiment five
A kind of detection means provided by the utility model, including colloidal gold strip as described above, when it is implemented, should
Detection means can also set shell, to play protective action to colloidal gold strip.
Finally it should be noted that：Various embodiments above is only to illustrate the technical solution of the utility model, rather than it is limited
System；Although the utility model is described in detail with reference to foregoing embodiments, one of ordinary skill in the art should
Understand：It can still modify to the technical scheme described in foregoing embodiments, either to which part or whole
Technical characteristic carries out equivalent substitution；And these modifications or replacement, the essence of appropriate technical solution is departed from this practicality newly
The scope of each embodiment technical scheme of type.
Claims (10)
- A kind of 1. colloidal gold strip, it is characterised in that including；Bottom plate, sample pad, colloidal gold pad, NC films, adsorptive pads and fixation Part；The bottom plate has a detection initial end and detection terminal, is set gradually from detection initial end to the detection terminal The sample pad, the colloidal gold pad, the NC films and the adsorptive pads；Wherein, the sample pad is partially covered in the colloidal gold pad, and the colloidal gold pad is partially covered on the NC films One end, the adsorptive pads are partially covered on the other end of the NC films；Covered with fixture on the sample pad, the colloidal gold pad and part NC films.
- 2. colloidal gold strip according to claim 1, it is characterised in that the NC films middle part detection line is coated with Antigen or antibody.
- 3. colloidal gold strip according to claim 1, it is characterised in that gold mark is coated with the colloidal gold pad Antigen or antibody.
- 4. colloidal gold strip according to claim 1, it is characterised in that the sample pad, colloidal gold pad, NC films and institute Adsorptive pads are stated to be connected with the base plate sticking respectively.
- 5. colloidal gold strip according to claim 1, it is characterised in that also including hemofiltration pad, it is arranged on the sample Between product pad and the colloidal gold pad, wherein, the sample pad is partially covered on the hemofiltration pad, and the hemofiltration pad part is covered Cover in the colloidal gold pad.
- 6. colloidal gold strip according to claim 5, it is characterised in that sample segment pad, hemofiltration pad, colloidal gold pad and Covered with fixture on the NC films of part.
- 7. according to the colloidal gold strip described in claim any one of 1-6, it is characterised in that the fixture uses transparent adhesive tape Band.
- 8. colloidal gold strip according to claim 1, it is characterised in that the colloidal gold pad is using coating gold labeling antibody Glass fibre or polyester.
- 9. colloidal gold strip according to claim 1, it is characterised in that the adsorptive pads use absorbent filter.
- 10. a kind of detection means, it is characterised in that including the colloidal gold strip described in claim any one of 1-9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720980726.5U CN207067142U (en) | 2017-08-07 | 2017-08-07 | Colloidal gold strip and detection means |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720980726.5U CN207067142U (en) | 2017-08-07 | 2017-08-07 | Colloidal gold strip and detection means |
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| Publication Number | Publication Date |
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| CN207067142U true CN207067142U (en) | 2018-03-02 |
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| CN201720980726.5U Active CN207067142U (en) | 2017-08-07 | 2017-08-07 | Colloidal gold strip and detection means |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113552339A (en) * | 2020-04-26 | 2021-10-26 | 格林生物医药科技(天津)有限公司 | Colloidal gold detection reagent card |
| CN113552338A (en) * | 2020-04-26 | 2021-10-26 | 格林生物医药科技(天津)有限公司 | Allergen specificity IgE antibody detection kit and preparation method thereof |
-
2017
- 2017-08-07 CN CN201720980726.5U patent/CN207067142U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113552339A (en) * | 2020-04-26 | 2021-10-26 | 格林生物医药科技(天津)有限公司 | Colloidal gold detection reagent card |
| CN113552338A (en) * | 2020-04-26 | 2021-10-26 | 格林生物医药科技(天津)有限公司 | Allergen specificity IgE antibody detection kit and preparation method thereof |
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