CN204028082U - The colloid gold test paper of a kind of Quantitative detection NGAL and TIMP-2 - Google Patents
The colloid gold test paper of a kind of Quantitative detection NGAL and TIMP-2 Download PDFInfo
- Publication number
- CN204028082U CN204028082U CN201420423962.3U CN201420423962U CN204028082U CN 204028082 U CN204028082 U CN 204028082U CN 201420423962 U CN201420423962 U CN 201420423962U CN 204028082 U CN204028082 U CN 204028082U
- Authority
- CN
- China
- Prior art keywords
- test paper
- pad
- timp
- ngal
- colloid gold
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The utility model discloses the colloid gold test paper of a kind of Quantitative detection NGAL and TIMP-2, described colloid gold test paper comprises support pad 5, be positioned at the nitrocellulose filter 1 in described support pad, one end of described nitrocellulose filter connects adsorptive pads 4, the other end connects conjugates pad 2, described conjugates pad connects sample pad 3, the below of described sample pad the other end is provided with the first fixed part 7, top is provided with the second fixed part 6, it is characterized in that, described nitrocellulose filter 1 middle part first detection line 8 and the second detection line 11 are coated with monoclonal or the polyclonal antibody of anti-NGAL or anti-TIMP-2 respectively, control line 9 is respectively equipped with on the both sides of described first detection line and the second detection line, described conjugates pad 2 is coated with NGAL and TIMP-2 antibody and the control line conjugates of gold mark.
Description
Technical field
The utility model belongs to clinical diagnose field, is specifically related to the colloid gold test paper of a kind of Quantitative detection NGAL and TIMP-2.
Background technology
Acute renal failure (AKI) is a clinical syndrome caused by Different types of etiopathogenises, and be change because of kidney circulatory failure or renal tubule and the sudden renal function of one that causes is lost, therefore kidney cannot get rid of the metabolic waste of health.When kidney cannot exercise normal function, can cause toxin, refuse and moisture are piled up in vivo, and cause acute renal failure.The possible AKI mark found successively in recent years comprises interleukin-18 (IL-18), cysteine proteinase inhibitor C (cystatin C), Kim1 (KIM-1), NGAL (NGAL) and tissue inhibitor of metalloproteinase-2 (tissue inhibitor of metalloproteinases-2, TIMP-2 etc.
Immune colloidal gold technique (Immune colloidal gold technique) is a kind of novel immunolabelling technique being applied to antigen-antibody using collaurum as tracer label thing.Collaurum be by gold chloride (HAuCl4) at reductive agent as under the effect such as white phosphorus, ascorbic acid, sodium citrate, tannic acid, polymerization becomes the gold grain of specific size, and becomes a kind of stable colloidal state due to electrostatic interaction, is called collaurum.Collaurum is electronegative under mild alkaline conditions, can be formed firmly be combined, because this combination is electrostatical binding, so do not affect the biological nature of protein with the positive charge group of protein molecule.Collaurum except with protein bound except, can also be combined with other biomacromolecules many.According to some physical behaviors of collaurum, as high electron density, grain size, shape and color reaction, add immunity and the biological characteristics of bond, thus make collaurum be widely used in the fields such as immunology, histology, pathology and cell biology.
In view of there is no Quantitative detection NGAL and TIMP-2 method at present, the purpose of this utility model is to provide and a kind ofly can be used for the colloid gold test paper of Quantitative detection NGAL and TIMP-2 and a kind of colloid gold test paper box that can be used for Quantitative detection NGAL and TIMP-2, and described colloid gold test paper and colloid gold test paper box have the advantages such as convenient and swift, simple to operate, result is accurate.
Utility model content
An object of the present utility model is to provide the colloid gold test paper of a kind of Quantitative detection NGAL and TIMP-2, the colloid gold test paper of Quantitative detection NGAL and TIMP-2 described in the utility model has the advantages such as convenient and swift, simple to operate, result is accurate, is suitable for clinical quick diagnosis.
The technical solution of the utility model is:
The colloid gold test paper of a kind of Quantitative detection NGAL and TIMP-2, comprise support pad 5, be positioned at the nitrocellulose filter 1 in described support pad, one end of described nitrocellulose filter connects adsorptive pads 4, the other end connects conjugates pad 2, described conjugates pad connects sample pad 3, the below of described sample pad the other end is provided with the first fixed part 7, top is provided with the second fixed part 6, it is characterized in that, described nitrocellulose filter 1 middle part first detection line 8 and the second detection line 11 are coated with monoclonal or the polyclonal antibody of anti-NGAL and anti-TIMP-2 respectively, control line 9 is respectively equipped with on the both sides of described first detection line and the second detection line, described conjugates pad 2 is coated with NGAL and TIMP-2 antibody and the control line conjugates of gold mark.
Preferably, described control line 9 is coated with anti-DNP antibody, and described control line conjugates is the collaurum of DNP-BSA mark.
Wherein said BSA refers to bovine serum albumin(BSA).
Preferably, described first fixed part 7 is double faced adhesive tape, and the second fixed part 6 is one side glue.
Preferably, described two control lines 9 and the first detection line 8 and the second detection line 11 are for be arrangeding in parallel.
Another object of the present utility model there is provided the colloid gold test paper box of a kind of Quantitative detection NGAL and TIMP-2 antibody, and the basis of the colloid gold test paper of quantitative detection NGAL and TIMP-2 antibody described in the utility model also comprises a shell 10.
The preferred plastic casing of described shell.
Described shell wraps up described test paper, exposes the first detection line 8 on sample pad 3, nitrocellulose filter 1 and the second detection line 11 and two control lines 9 and adsorptive pads 4.
Paper box described in the utility model has the advantage being convenient for carrying and storing.
Quantitative detection NGAL described in the utility model and the colloid gold test paper of TIMP-2 antibody or the principle of work of paper box are: adopt two-way effluent immune response principle, be prepared from by double antibody sandwich method.NGAL and TIMP-2 antigen during inspection in sample first respectively with NGAL and the TIMP-2 antibody conjugates generation immune response on coupling pad, formed immune complex.Thereafter immune complex is along with sample chromatographic flow on NC Nitroncellulose film, when immune complex chromatography is to detection zone (NGAL and TIMP-2 detection line) on NC Nitroncellulose film, reacts with anti-NGAL and the TIMP-2 antibody be coated in advance on NC Nitroncellulose film thus be fixed on the detection line of NC Nitroncellulose film respectively.NGAL and TIMP-2 in sample is more, and the compound on detection line is more, and the optical density value on band is higher.Simultaneously, in testing process, control line conjugates (i.e. the collaurum of DNP-BSA mark), also can with sample chromatography on NC Nitroncellulose film, when chromatography is to the control line of NC Nitroncellulose film, DNP-BSA collaurum can react with the anti-DNP antibody be coated in advance on NC Nitroncellulose film thus be fixed on check plot (control line).Because collaurum is red, the DNP-BSA colloid gold immune compound be thus fixed in the anti-DNP on NC Nitroncellulose film control line will show redness.When not containing NGAL and TIMP-2 in sample, then only show two control lines.When sample is containing NGAL and TIMP-2, detection line will show respectively clearly.
After reaction terminates, multifunction immunity detector (auspicious Lay bioengineering (Shenzhen) company limited) is utilized the optical density of control line and detection line to be analyzed, and by analyze the result obtained and carry out computing, thus obtain relative optical density number (RI).Then detector calculates according to the concentration of the typical curve be set in advance in detector to NGAL and TIMP-2 and shows result, represents in units of ng/mL.
Accompanying drawing explanation
Fig. 1 is the structural representation of a kind of preferred implementation of the colloid gold test paper of Quantitative detection NGAL and TIMP-2 described in the utility model.
Wherein, 1: nitrocellulose filter, 2: conjugates pad, 3: sample pad, 4: adsorptive pads, 5: support pad, 6: the second fixed parts, 7: the first fixed parts, 8: the first detection lines, 9: control line, 11: be the second detection line.
Fig. 2 is the diagram of a kind of preferred implementation of the colloid gold test paper box of Quantitative detection NGAL and TIMP-2 described in the utility model.
Wherein, 3: sample pad, 8: detection line, 9: control line, 4: adsorptive pads, 10: shell.
Embodiment
Below in conjunction with accompanying drawing, the colloid gold test paper of Quantitative detection NGAL and TIMP-2 described in the utility model or paper box are described in detail, can not be interpreted as it is to restriction of the present utility model.The material used in following examples and reagent unless otherwise indicated, are common commercially available.
[embodiment 1] prepares the colloid gold test paper of Quantitative detection NGAL and TIMP-2
Colloid gold test paper structure as shown in Figure 1, comprise support pad 5, be positioned at the nitrocellulose filter 1 in described support pad, stacked connection adsorptive pads 4 above one end of described nitrocellulose filter, stacked connection conjugates pad 2 above the other end, stacked connection sample pad 3 above described conjugates pad one end away from nitrocellulose filter 1, bonding first fixed part 7 in below of described sample pad the other end, be preferably double faced adhesive tape, bonding first fixed part 6 in top, be preferably one side glue, it is characterized in that, described nitrocellulose filter 1 middle part first detection line 8 and the second detection line 11 are coated with monoclonal or the polyclonal antibody of NGAL or anti-TIMP-2 respectively, control line 9 is respectively equipped with on the both sides of described first detection line and the second detection line, described conjugates pad 2 is coated with NGAL and TIMP-2 antibody and the control line conjugates of gold mark.
Described control line 9 is coated with anti-DNP antibody, and described control line conjugates is the collaurum of DNP-BSA mark.
Wherein, nitrocellulose filter 1 for fixing coated antibody, is also immunoreactive nidus in colloid gold test paper simultaneously; First detection line 8 and the second detection line 11 use the damping fluid such as 1*PBS, methyl alcohol to be diluted to the concentration of 0.5mg/ml NGAL and TIMP-2 antibody respectively, gets 1ul/cm and line on described nitrocellulose filter 1, dry, to obtain final product; Control line 9 uses the damping fluid such as 1*PBS, methyl alcohol to be diluted to the concentration of 0.5mg/ml anti-DNP antibody, gets 1ul/cm and line on described nitrocellulose filter 1, dry, to obtain final product.
Wherein, the starting material of described conjugates pad 2 are glass fiber filter, glass fiber filter for the preparation of conjugates pad is put into pre-Block buffer and (comprises the casein of 0.1-1%, the PEG of the bovine serum albumin(BSA) of 0.5-5%, the boric acid of 0.005-0.5%, 0.01-0.2%) middle immersion taking-up after 5 minutes, dry, NGAL and the TIMP-2 monoclonal antibody marked by gold with Biodot coating instrument or polyclonal antibody and control line conjugates are coated on conjugates pad 2, drying, to obtain final product.Preferably, the pre-Block buffer of described conjugates pad 2 comprises the casein of 0.8%, the bovine serum albumin(BSA) of 4%, the boric acid of 0.007%, the PEG of 0.1%.
Wherein, described sample pad 3 can play preliminary filtering function by liquid towards sample.After sample pad is soaked 5 minutes with 141 confining liquids (comprising: the casein of the Tris of 0.1-1%, 0.1-1%), dry, to obtain final product.Preferably, described 141 confining liquids comprise: the Tris of 0.3%, the casein of 0.8%.
Above-mentioned each building block is pasted onto in support pad 5 by structure shown in Fig. 1, obtains the colloid gold test paper of Quantitative detection NGAL and TIMP-2.
[embodiment 2] is with colloid gold test paper Quantitative detection NGAL and TIMP-2 described in the utility model
Get the colloid gold test paper of preferred a kind of Quantitative detection NGAL and TIMP-2 of the utility model, as shown in Figure 1, described colloid gold test paper comprises support pad 5, be positioned at the nitrocellulose filter 1 in described support pad, stacked connection adsorptive pads 4 above one end of described nitrocellulose filter, stacked connection conjugates pad 2 above the other end, stacked connection sample pad 3 above described conjugates pad one end away from nitrocellulose filter 1, bonding first fixed part 7 in below of described sample pad the other end, be preferably double faced adhesive tape, bonding second fixed part 6 in top, be preferably one side glue, it is characterized in that, described nitrocellulose filter 1 middle part first detection line 8 and the second detection line 11 are coated with monoclonal or the polyclonal antibody of anti-NGAL or anti-TIMP-2 respectively, control line 9 is respectively equipped with on the both sides of described first detection line and the second detection line, described conjugates pad 2 is coated with NGAL and TIMP-2 antibody and the control line conjugates of gold mark.Preferably, described control line 9 is coated with anti-DNP antibody, and described control line conjugates is the collaurum of DNP-BSA mark.Detect according to the following steps: the patient urine sample 1) gathered, as Cord blood sample need return to room temperature.2) testing sample is added on sample pad 3, reaction.3) interpretation, is placed in multifunction immunity detector by described colloid gold test paper, result of determination in SSJ-2 or MINI (auspicious Lay bioengineering (Shenzhen) company limited).
[embodiment 3] is with colloid gold test paper box Quantitative detection NGAL and TIMP-2 described in the utility model
Get the colloid gold test paper box of preferred a kind of Quantitative detection NGAL and TIMP-2 of the utility model, as depicted in figs. 1 and 2, described colloid gold test paper comprises support pad 5, be positioned at the nitrocellulose filter 1 in described support pad, one end of described nitrocellulose filter connects adsorptive pads 4, the other end connects conjugates pad 2, described conjugates pad connects sample pad 3, bonding first fixed part 7 in below of described sample pad the other end, be preferably double faced adhesive tape, bonding second fixed part 6 in top, be preferably one side glue, it is characterized in that, described nitrocellulose filter 1 middle part first detection line 8 and the second detection line 11 are coated with monoclonal or the polyclonal antibody of anti-NGAL or TIMP-2 respectively, control line 9 is respectively equipped with on the both sides of described first detection line and the second detection line, described conjugates pad 2 is coated with NGAL and TIMP-2 antibody and the control line conjugates of gold mark.。Preferably, described control line 9 is coated with anti-DNP antibody, and described control line conjugates is the collaurum of DNP-BSA mark.Described colloid gold test paper also comprises a shell 10, and described shell wraps up described test paper, exposes detection line 8 on sample pad 3, nitrocellulose filter 1 and two control lines 9 and adsorptive pads 4.Detect according to the following steps: the patient urine sample 1) gathered, as Cord blood sample need return to room temperature.2) testing sample is added on sample pad 3, reaction.3) interpretation, is placed in multifunction immunity detector by described colloid gold test paper, result of determination in SSJ-2 or MINI (auspicious Lay bioengineering (Shenzhen) company limited).
Claims (5)
1. the colloid gold test paper of Quantitative detection NGAL and TIMP-2, comprise support pad (5), be positioned at the nitrocellulose filter (1) in described support pad, one end of described nitrocellulose filter connects adsorptive pads (4), the other end connects conjugates pad (2), described conjugates pad connects sample pad (3), the below of described sample pad the other end is provided with the first fixed part (7), top is provided with the second fixed part (6), it is characterized in that, described nitrocellulose filter (1) middle part first detection line (8) and the second detection line (11) are coated with monoclonal or the polyclonal antibody of anti-NGAL or anti-TIMP-2 respectively, control line (9) is respectively equipped with on the both sides of described first detection line and the second detection line, described conjugates pad (2) is coated with NGAL and TIMP-2 antibody and the control line conjugates of gold mark.
2. colloid gold test paper as claimed in claim 1, it is characterized in that, described control line (9) is coated with anti-DNP antibody, and described control line conjugates is the collaurum of DNP-BSA mark.
3. colloid gold test paper as claimed in claim 1, it is characterized in that, described first fixed part (7) is double faced adhesive tape, and the second fixed part (6) is one side glue.
4. the colloid gold test paper according to any one of claim 1-3, it is characterized in that, described colloid gold test paper also comprises a shell (10) outward, described shell wraps up outside described colloid gold test paper, exposes the first detection line (8) on sample pad (3), nitrocellulose filter (1), the second detection line (11), two control lines (9) and adsorptive pads (4).
5. colloid gold test paper as claimed in claim 4, is characterized in that, described two control lines (9) and the first detection line (8) and the second detection line (11) are for be arrangeding in parallel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420423962.3U CN204028082U (en) | 2014-07-30 | 2014-07-30 | The colloid gold test paper of a kind of Quantitative detection NGAL and TIMP-2 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420423962.3U CN204028082U (en) | 2014-07-30 | 2014-07-30 | The colloid gold test paper of a kind of Quantitative detection NGAL and TIMP-2 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204028082U true CN204028082U (en) | 2014-12-17 |
Family
ID=52067924
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420423962.3U Active CN204028082U (en) | 2014-07-30 | 2014-07-30 | The colloid gold test paper of a kind of Quantitative detection NGAL and TIMP-2 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204028082U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104880563A (en) * | 2015-04-08 | 2015-09-02 | 上海盛复源生物医药有限公司 | NGAL rapid detection reagent and preparation method thereof |
| CN105510577A (en) * | 2015-11-27 | 2016-04-20 | 上海艾瑞德生物科技有限公司 | Method for rapidly and quantitatively detecting multiple analytes in blood by adopting multi-point calibration |
| CN105911274A (en) * | 2016-06-12 | 2016-08-31 | 吉林大学 | Immunochromatography device for synchronously and quantitatively detecting different molecular forms of human neutrophil lipocalin (HNL) and preparation method thereof |
-
2014
- 2014-07-30 CN CN201420423962.3U patent/CN204028082U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104880563A (en) * | 2015-04-08 | 2015-09-02 | 上海盛复源生物医药有限公司 | NGAL rapid detection reagent and preparation method thereof |
| CN105510577A (en) * | 2015-11-27 | 2016-04-20 | 上海艾瑞德生物科技有限公司 | Method for rapidly and quantitatively detecting multiple analytes in blood by adopting multi-point calibration |
| CN105911274A (en) * | 2016-06-12 | 2016-08-31 | 吉林大学 | Immunochromatography device for synchronously and quantitatively detecting different molecular forms of human neutrophil lipocalin (HNL) and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103869079B (en) | A kind of colloid gold test paper of Quantitative detection CBP-35 | |
| JP4934147B2 (en) | Test equipment for rapid diagnosis | |
| CN104502586B (en) | A kind of immunochromatography detection method and test paper | |
| CN102449482B (en) | Method for signal amplification during lateral-flow analysis | |
| JP5683543B2 (en) | Chromatograph kit and chromatographic method | |
| CN107085116A (en) | It is a kind of to detect kit of calprotectin and preparation method thereof in human faecal mass sample | |
| CN205317786U (en) | A gold -labeled antibody test paper strip for early diabetic nephropathy detects | |
| CN103926401A (en) | Immunofluorescence test paper strip for rapidly and quantitatively measuring IGFBP-7 and TIMP-2 and preparation method thereof | |
| CN106066399A (en) | The preparation method of the colloidal gold strip of detection early diabetic nephropathy | |
| EP2713164A1 (en) | Chromatography method and kit | |
| CN205229167U (en) | Quick quantitative detection AMH's test paper | |
| CN204028082U (en) | The colloid gold test paper of a kind of Quantitative detection NGAL and TIMP-2 | |
| CN104931696A (en) | Immunochromotographic analysis kit, immunochromotographic analysis device, and immunochromotographic analysis method | |
| CN105793707A (en) | Immunochromatography-assisted detection method | |
| CN205539004U (en) | Detect NGAL and glycated haemoglobin's test paper | |
| JP5066498B2 (en) | Assay method | |
| CN203786123U (en) | Colloidal gold test paper for rapid detection of Aspirin efficacy | |
| US9709566B2 (en) | Chromatography method, and chromatography kit | |
| CN103940990A (en) | Sensitivity-enhanced gold-labeled immunochromatographic strip and preparation method thereof | |
| CN203881772U (en) | Colloidal gold test paper for quickly and quantitatively detecting L-FABP | |
| JP2010071828A (en) | Method for detecting substance to be examined | |
| CN203881779U (en) | Quantitative detection system of colloidal gold test paper box | |
| CN104330568A (en) | Colloidal gold immunity chromatography kit for detecting cyanophycean toxin | |
| CN203732547U (en) | Colloidal gold test paper for rapid and quantitative detection of -3(Gal-3) | |
| CN203909042U (en) | Colloidal gold test paper for detecting IGFBP-7 (insulin-like growth factor binding protein 7) and TIMP-2 (tissue inhibitor of metalloproteinase 2) rapidly and quantitatively |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address | ||
| CP03 | Change of name, title or address |
Address after: 518000 Nanshan Medical Device Industrial Park BF02-BF04, No. 1019, Nanhai Avenue, Yanshan Community, Merchants Street, Nanshan District, Shenzhen, Guangdong Province Patentee after: ruilai bioengineering Co.,Ltd. Address before: 518000 B501 Nanshan Medical Equipment Industrial Park, Nanshan Nanhai Avenue, Shenzhen, Guangdong Province Patentee before: RELIA BIOLOGICAL ENGINEERING (SHENZHEN) CO.,LTD. |