CN103529224B - Detect human brain natriuretic peptide and the Quantitative detection device of N-terminal plasma pro-brain natriuretic peptide levels and detection method simultaneously - Google Patents
Detect human brain natriuretic peptide and the Quantitative detection device of N-terminal plasma pro-brain natriuretic peptide levels and detection method simultaneously Download PDFInfo
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/32—Cardiovascular disorders
- G01N2800/325—Heart failure or cardiac arrest, e.g. cardiomyopathy, congestive heart failure
Abstract
A kind of detection human brain natriuretic peptide and the Quantitative detection device of N-terminal plasma pro-brain natriuretic peptide levels and detection method simultaneously, it is characterised in that device is got stuck by separate human brain natriuretic peptide (BNP) and N-terminal plasma pro-brain natriuretic peptide levels (NT ProBNP) Test paper, duplex and the immunochromatography sentence read result recorder of built-in respective standard curve and decision method forms.Immune chromatography result interpretation recorder is a kind of Systems for optical inspection, for the quantitative judgement to testing result, BNP and NT ProBNP detection range is respectively 0.1 10ng/mL and 0.2 20ng/mL.Detection completed in 15 20 minutes.Have joint-detection, easy and simple to handle, reaction is quick, result is accurate, is suitable for the advantages such as Site Detection, is suitable for the clinical needs detecting the most accurate, simplicity.
Description
Technical field
Patent of the present invention relates to medical clinic applications technical field, particularly relates to one and quickly detects with immune colloid gold
Human brain natriuretic peptide and the Quantitative detection device of N-terminal plasma pro-brain natriuretic peptide levels and detection method is detected while method preparation.
Background technology
Brain natriuretic peptide (BNP) be first by Japanese scholars Sudoh equal within 1988, separating from pig brain thus gain the name,
Also referred to as B-typeNatriuretic Peptide.Mankind's brain natriuretic peptide (BNP) is distributed widely in brain, heart, lungs, alimentary canal, urogenital tract
In organizing, wherein the level with heart is the highest.Cardiac muscle cell is not to be directly synthesized BNP, but first synthesizes pro-BNP,
Pro-BNP has 108 amino acid, can decompose in blood, and the fragment decomposed is 76 amino acid of its N end,
I.e. NT-ProBNP, remaining 32 amino acid, i.e. BNP.
Research finds, early diagnosis, early intervention and the prognosis of heart failure are had very great help by BNP.Revise in calendar year 2001
ESC's heart failure practice guidelines in, using brain natriuretic peptide as the instrument of kit for diagnosing heart failure.2005 Europe and
The guide of the U.S., has affirmed brain natriuretic peptide effect in kit for diagnosing heart failure the most further.Due to BNP and NT-ProBNP from
In same precursor, there is close contact between the two.The U.S.'s " clinical chemistry " magazine delivered one in 2007
Paper: " BNP and NT-proBNP diagnosis accuracy in acute and chronic HF compares ", draw through substantial amounts of Document system
As drawn a conclusion: BNP and NT-proBNP chemical examination has higher diagnosis accuracy in the diagnosis of acute and chronic HF and has
There is higher correlation.
From clinical diagnosis angle analysis, difference main for BNP and NT-proBNP is as follows: 1) removing approach in vivo is not
Mainly be combined then by endocytosis and lysosomal degradation by removing acceptor with natriuretic peptide with: the removing of BNP, the most a small amount of
BNP is removed by kidney, and when renal function lacks, neutral endopeptidase (NEP) also can open the circulus of BNP
And it is purged；The unique channel that NT-proBNP removes is glomerular filtration, and renal function disappearance occurs to NT-proBNP
Metabolic effect very big.2) half-life is different: the half-life of BNP is 22 minutes, and the half-life of NT-proBNP is
120 minutes.From the point of view of clinical examination, NT-proBNP is the most stable, brings convenience to detection,
But from the point of view of clinical practice, the half-life shorter for BNP more can react conditions of patients change, beneficially clinical monitoring in time
Result for the treatment of, thus bring more preferable using value to clinic.3) the cutoff Zhi Youhen great district of BNP and NT-proBNP
Not, the cutoff value having diagnostic value that BNP generally acknowledges is 0.1ng/mL, and NT-proBNP is 0.3ng/mL.
The reagent of BNP and NT-ProBNP detection at present has application in clinic, but not yet has and detect both indexs simultaneously
Quick detection reagent/device occurs.Detection simultaneously can realize the mutual supplement with each other's advantages of two reagent, more be sure of testing result,
The false positive caused because of some cross reaction can be got rid of or false negative that molecule isomery causes；Whether can analyze is kidney
The false positive of the NT-ProBNP that functional metabolism obstacle causes, can improve the sensitivity of detection quick to heart failure.
Summary of the invention
It is an object of the present invention to provide a kind of detection human brain natriuretic peptide and the Quantitative detection device of N-terminal plasma pro-brain natriuretic peptide levels simultaneously
And detection method, it is used for detecting BNP and NT-ProBNP in serum, blood plasma and whole blood sample.Have easy and simple to handle,
Reaction is quickly, result is accurate, credible, be suitable for the advantages such as Site Detection.Summary of the invention is as follows:
A kind of detection human brain natriuretic peptide and the Quantitative detection device of N-terminal plasma pro-brain natriuretic peptide levels and detection method simultaneously, it is special
Levy and be that device is detected examination by separate human brain natriuretic peptide (BNP) and N-terminal plasma pro-brain natriuretic peptide levels (NT-ProBNP)
Paper, duplex get stuck and the immunochromatography sentence read result recorder composition of built-in respective standard curve and decision method.
Described detection device, it is characterised in that BNP detection reagent test strip is resisted by the anti-human BNP containing colloid gold label
The colloidal gold pad of body, be coated with pairing BNP antibody (T line) and the NC film of anti-mouse IgG (C line), sample pad,
Sample suction pad and plastic bottom board composition.NT-ProBNP detection reagent test strip is by the anti-human NT-ProBNP containing colloid gold label
The colloidal gold pad of antibody, be coated with pairing NT-ProBNP antibody (T line) and the NC film of anti-mouse IgG (C line),
Sample pad, sample suction pad and plastic bottom board composition.The width of each test paper is 3-5mm, a length of 7-8cm.Sample pad,
Colloidal gold pad, NC film and sample suction pad are arranged in order to the other end from plastic bottom board one end.
Described detection device, it is characterised in that duplex gets stuck and comprises upper cover and two parts of lower cover, and inside can be placed in parallel
Article two, Test paper.The bottom counter sample pad part of upper cover of getting stuck is provided with sample well and dilution fluid apertures, is used for adding sample
Basis and dilution, be provided with viewing window in the middle part of the mid portion reply test paper NC film of the upper cover that gets stuck, be used for observing NC film
On C, T line, it is determined that result.
Described detection device, it is characterised in that described NC film is the film of a kind of loose structure being made up of celluloid,
Aperture is 8-15um.Dilution fluid cushion is glass fibre membrane or non-woven fabrics, and sample suction pad is absorbent filter.
Described detection device, it is characterised in that the NC film preparation process being coated with pairing antibody is: with 0.01M pH7.4
BNP or the NT-ProBNP antibody of pairing is configured to suitable concentration by phosphate buffer (PBS), at spray film instrument at NC
On film, the parameter with 1.2-1.4ul/cm is rule, and is coated T line, is coated the anti-of 1-2mg/ml on NC film top simultaneously
Mouse IgG is as C line.It is dried, standby.
Described detection device, it is characterised in that the preparation process of colloidal gold pad is: take 100ml collaurum liquid and be placed in beaker,
It is adjusted to pH7.0-8.0 with 0.2M K2C03, adds 1-2mg mark BNP or NT-ProBNP antibody, be stirred at room temperature
1 hour, closing, 12000rpm is centrifuged 30 minutes, abandons supernatant, redissolves to 60ml with working solution, then divides with metal spraying machine
It is sprayed on the most equably in colloidal gold pad, drying for standby.
Described detection device, its feature in detection card assembly method is: under conditions of relative humidity is less than 30%, take
Plastic bottom board, is pasted onto the middle part of base plate by the NC film being coated BNP or NT-ProBNP, viscous in NC film T line side
Patch colloidal gold pad, pastes sample pad at colloidal gold pad opposite side；Sample suction pad is pasted in NC film C line side；Each paste composition
Interface mutually laminates 1-2mm, and the big plate pasted is cut into the wide test strips of 3-5mm.Then two kinds of test strips are placed respectively
In the groove getting stuck lower cover, then cover upper cover, compress, complete assembling.
Described detection device, it is characterised in that immune chromatography result interpretation recorder is a kind of Systems for optical inspection, built-in
Calibration curve and decision method to BNP and NT-ProBNP test paper, for the quantitative judgement to testing result, to BNP
It is respectively 0.1-10ng/mL and 0.2-20ng/mL with NT-ProBNP detection range.
Described detection device, it is characterised in that detection method is: during detection by the tested sample of 50ul such as serum, blood plasma,
Whole blood joins in sample aperture, then adds 50ul sample diluting liquid, in 15-20 minute, with exempting from dilution fluid apertures
Testing result is judged by epidemic disease tomographic results interpretation recorder.
The invention has the beneficial effects as follows: a kind of detection human brain natriuretic peptide and the fast quantification of N-terminal plasma pro-brain natriuretic peptide levels simultaneously are provided
Detection device and detection method, be used for detecting BNP and NT-ProBNP in serum, blood plasma and whole blood sample.There is behaviour
Make the advantages such as easy, reaction is quick, result is accurate, credible, applicable Site Detection.
Accompanying drawing illustrates:
Fig. 1 is Test paper structure chart
Fig. 2 is dual card shell structure figure
Detailed description of the invention
Embodiment: the Quantitative detection device preparation of human brain natriuretic peptide and N-terminal plasma pro-brain natriuretic peptide levels and utilization
1 main material
1.1 biological raw material BNP and NT-ProBNP pairing antibody, buy from HYTEST company of Finland；Mouse-anti human IgG
Antibody, sheep anti-mouse igg: self-control；Gold chloride: Sigma Products；NC film: Sartorius Products；Hydrolysis
Casein, polyethylene glycol PEG20000:Sigma product.Other common agents is AR.
1.2 clinical samples are obtained at relevant hospital by company, totally 120 parts, are wherein diagnosed as the Blood of Patients final proof of heart failure
This 60 parts, BNP detected value from 0.2-8ng/mL, NT-ProBNP detected value from 0.2-20ng/mL.Normal human serum sample
These 60 parts, BNP and NT-ProBNP value does not detects.
1.3 duplexs get stuck: designed by our company, and associated companies produces on request, provides.
1.4 immune chromatography result knot interpretation recorders: model: NS3001, Newscen Coast Bio-Pharmaceutical Co., Ltd.
Product.
2 methods
2.1BNP and NT-ProBNP antibody colloidal gold mark uses gold chloride-sodium citrate to prepare a diameter of 40
The colloidal gold solution of ± 5nm, takes three parts of colloidal gold solutions, with 0.2M K2CO3, solution is transferred to pH7.0, pH7.5 respectively
And pH8.0, then collaurum is slowly stirred, adds 5ug, 10ug, 20ug by BNP labelled antibody by every ml solution
Join in solution, continue stirring 30 minutes, be then added to the hydrolysis junket of the PEG2000 and 0.5% of final concentration of 0.5%
Albumen is closed, and mark is centrifuged with 12000g after terminating, and abandons supernatant, and precipitation is redissolved to different ratio by 60% original volume
Collaurum working solution in (pH8.0, containing caseinhydrolysate, sheep blood serum, sucrose and surfactant).Then glue will be marked
Body gold solution presses 1ml solution paving 20cm2Ratio be loaded onto on non-woven fabrics, in temperature 20-25 DEG C, relative humidity is at ＜ 30%
Drying room be dried 3-5 hour, make colloidal gold pad.NT-ProBNP antibody labeling method is consistent with BNP, simply adds
Antibody be NT-ProBNP antibody.
2.2NC film is coated, with 0.01M pH7.4PBS, the BNP antibody of pairing is configured to 0.8, and 1.0 and 1.2mg/mL,
Sheep anti-mouse igg is diluted to 1mg/ml, 2mg/ml respectively, then carries out respectively by 1.4ul/cm on NC film with spray film instrument
Line is coated, and by NC film in temperature 20-25 DEG C after being coated, relative humidity is dried 3-5 at the drying room of ＜ 30%
Hour.NT-ProBNP antibody method for coating is consistent with BNP, simply preparation for NT-ProBNP antibody.
2.3, under conditions of relative humidity is less than 30%, takes plastic bottom board, will be coated the NC of BNP or NT-ProBNP
Film is pasted onto the middle part of base plate, pastes colloidal gold pad in NC film T line side, pastes sample pad at colloidal gold pad opposite side；
Sample suction pad is pasted in NC film C line side；Each paste composition interface mutually laminates 1-2mm, and the big plate pasted is cut into 3-5mm
Wide test strips.Then two kinds of test strips are respectively placed in the groove of the lower cover that gets stuck, then cover upper cover, compress, complete
Become assembling.
Concentration not isolabeling, coated reagent are combined pairing by 2.4 detection clipping technique parameter testings, prepare sample,
Utilize quality-control product that reagent is tested, find best of breed.
2.5 built-in calibration curve curves and parameter are arranged after determining test paper technological parameter, respectively with 0, and 0.1,0.5,
1.5,4, the BNP standard items of 8ng/mL reagent is measured, the standard items of variable concentrations demonstrate varying strength colour band,
With immunochromatography interpretation recorder by after the color band digital of respective strengths, calculate calibration curve, and input immunochromatography
In result interpretation recorder, complete BNP calibration curve parameter and arrange；Respectively with 0,0.2,0.5,2,8,20ng/mL
NT-ProBNP standard items reagent is measured, the standard items of variable concentrations demonstrate varying strength colour band, with immunity
Chromatography interpretation recorder by after the color band digital of respective strengths, calculates calibration curve, and inputs immune chromatography result and sentence
In read record instrument, complete NT-ProBNP calibration curve parameter and arrange.The Cutoff value of BNP is set to 0.1ng/mL,
The Cutoff value of NT-ProBNP is set to 0.3ng/mL, if two kinds of detections are feminine gender, synthesis result is judged to feminine gender, if
Two kinds of result test positive, synthesis result is judged to the positive.If two kinds of results are detected as inconsistent, it is determined that can for result
Doubt, complete result critical parameter and arrange.
2.6 detection methods 1) detection reagent and sample are balanced to room temperature, take out test card, keep flat；2) the most smart
Really draw 50 μ l serum, blood plasma or whole blood sample, join in two sample aperture, the most respectively at the dilution of bottom
Hole adds 50 μ L Sample dilution (PBS), in 15-20 minute, quantitatively judges result with immune chromatography result interpretation record；
3) after setting instrument relevant parameter, test card is put into storehouse to detect, instrument display is gone out respectively BNP and
The quantified results of NT-ProBNP sample concentration；And the comprehensive detection result to heart failure.
After prepared by 2.7 clinical sample detection reagent, by detection method, all clinical samples are detected, and point
Analysis testing result.
3 results
3.1 test paper parameter determinations are according to the testing result of sample, it is determined that BNP and NT-ProBNP antibody labeling pH
Value is 7.0-8.0, no significant difference；Optimum mark amount is 5-10ug/ml colloidal gold solution；Optimal collaurum working solution
For 0.1MTris.Cl buffer solution, pH7.5, containing 0.2% caseinhydrolysate, 6% sucrose, 0.5%Tween20；Optimal bag
The concentration of antigen be BNP be 0.8mg/mL, NT-ProBNP be 1.2mg/mL.The optimal decision time of testing result is
15-20 minute.But above parameter may suitably adjust by needs because of biological raw material activity change when preparing different batches product.
120 parts of clinical samples are detected by 3.2 clinical samples detections, two kinds of detections be the positive for 58 examples,
Be feminine gender for 57 examples, compare with clinic, desired value positive, negative is 100%.Other 5 examples are suspect results.Combine
The concordance rate closing result is 95.8%.Comparing with individually detection, concordance rate is suitable, but desired value positive, negative substantially rises.
In quantitative context of detection, correlation coefficient r is all higher than 0.95, and t checks P ＞ 0.05, inconsistent detection outlier ＜ 2%.Fixed
Amount Detection results is preferable.Can be determined that the functional of detection device by result above, have easy and simple to handle, reaction is fast
Speed, result are accurate, credible, be suitable for the advantages such as Site Detection, are suitable for the clinical needs to detection the most accurate, easy.
Claims (4)
1. detect human brain natriuretic peptide and a Quantitative detection device for N-terminal plasma pro-brain natriuretic peptide levels simultaneously, its
It is characterised by: described device is by separate human brain natriuretic peptide (BNP) and N-terminal plasma pro-brain natriuretic peptide levels
(NT-ProBNP) Test paper, duplex get stuck and the exempting from of built-in respective standard curve and decision method
Epidemic disease chromatography sentence read result recorder composition, described immunochromatography sentence read result recorder is for testing result
Rational judgment, BNP and NT-ProBNP detection range is respectively 0.1-10ng/mL and 0.2-20ng/mL；
Described BNP detection reagent test strip by the colloidal gold pad of the anti-human BNP antibody containing colloid gold label,
It is coated with pairing BNP antibody (T line) and NC film, sample pad, the sample suction pad of anti-mouse IgG (C line) and moulds
Material base plate composition；
Described NT-ProBNP detection reagent test strip is by the anti-human NT-ProBNP antibody containing colloid gold label
Colloidal gold pad, be coated with pairing NT-ProBNP antibody (T line) and the NC film of anti-mouse IgG (C line), sample
Product pad, sample suction pad and plastic bottom board composition；
The width of each test paper is 3-5mm, a length of 7-8cm；Sample pad, colloidal gold pad, NC film and
Sample suction pad is arranged in order to the other end from plastic bottom board one end；
The NC film preparation process being coated with pairing antibody is: with 0.01M PH7.4 phosphate buffer (PBS)
BNP or the NT-ProBNP antibody of pairing is configured to suitable concentration, spray film instrument on NC film with
The parameter of 1.2-1.4 μ L/cm is rule, and is coated T line, is coated 1-2mg/mL's on NC film top simultaneously
Anti-mouse IgG is as C line；It is dried, standby；
The preparation process of colloidal gold pad is: takes 100mL collaurum liquid and is placed in beaker, uses 0.2M K2CO3
It is adjusted to pH7.0-8.0, adds 1-2mg mark BNP or NT-ProBNP antibody, be stirred at room temperature 1 hour；
Closing, 12000rpm is centrifuged 30 minutes, abandons supernatant, redissolves to 60mL with working solution, then uses metal spraying machine
It is sprayed on the most equably in colloidal gold pad, drying for standby；
Detection method is: join in sample aperture by the tested sample of 50 μ L during detection, then in dilution
Fluid apertures adds 50 μ L sample dilutions, in 15-20 minute, with immune chromatography result interpretation recorder
Testing result is judged.
Detect device the most according to claim 1, it is characterised in that: described duplex gets stuck and comprises
Lid and two parts of lower cover, interior parallel places two Test papers；Get stuck the bottom counter sample of upper cover
Pad part is provided with sample well and dilution fluid apertures, is used for adding sample and dilution, the centre of the upper cover that gets stuck
It is provided with viewing window, for observing C, T line on NC film, it is determined that result in the middle part of part reply test paper NC film.
Detection device the most according to claim 1 and 2, it is characterised in that: described NC film is a kind of
The film of the loose structure being made up of celluloid, aperture is 8-15 μm；Sample suction pad is absorbent filter.
Detection device the most according to claim 1 and 2, it is characterised in that: detection card assembly method
For: under conditions of relative humidity is less than 30%, takes plastic bottom board, will be coated BNP or NT-ProBNP
NC film be pasted onto the middle part of base plate, paste colloidal gold pad in NC film T line side, colloidal gold pad another
Sample pad is pasted in side；Sample suction pad is pasted in NC film C line side；Each paste composition interface mutually laminates 1-2mm,
The big plate pasted is cut into the wide test strips of 3-5mm；Then two kinds of test strips are respectively placed under getting stuck
In the groove of lid, then cover upper cover, compress, complete assembling.
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| CN105044363B (en) * | 2015-06-01 | 2017-03-15 | 上海凯创生物技术有限公司 | A kind of brain natriuretic peptide gold-immunochromatographyreagent reagent for assay box |
| CN107389955A (en) * | 2017-08-03 | 2017-11-24 | 天津中新科炬生物制药股份有限公司 | The immune colloid gold detection means and detection method of HCG detection ranges can be widened |
| CN107884575A (en) * | 2017-11-01 | 2018-04-06 | 上海凯创生物技术有限公司 | Amino terminal-pro brain natriuretic peptide detection reagent card, kit and application thereof |
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| CN1764839A (en) * | 2003-03-28 | 2006-04-26 | 维达实验室 | Solid-phase immunochromatographic methods |
| CN201087839Y (en) * | 2007-03-30 | 2008-07-16 | 万华普曼生物工程有限公司 | Brain sodium peptide color particle diagnosis test paper |
| CN102116770A (en) * | 2009-12-31 | 2011-07-06 | 北京热景生物技术有限公司 | Immunochromatography rapid kit and production method thereof |
| CN102692512A (en) * | 2012-06-26 | 2012-09-26 | 南京基蛋生物科技有限公司 | Double-antibody sandwich rapid detection kit of N-terminal pro-brain natriuretic peptide (NT-proBNP) |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AT500800B1 (en) * | 2004-09-08 | 2008-07-15 | Biomedica Medizinprodukte Gmbh | PROCESS FOR DETERMINING PROBNP |
| EP2539695B1 (en) * | 2010-02-23 | 2022-06-22 | B.R.A.H.M.S GmbH | A method for determining a correct test performance for a flow test element |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1764839A (en) * | 2003-03-28 | 2006-04-26 | 维达实验室 | Solid-phase immunochromatographic methods |
| CN201087839Y (en) * | 2007-03-30 | 2008-07-16 | 万华普曼生物工程有限公司 | Brain sodium peptide color particle diagnosis test paper |
| CN102116770A (en) * | 2009-12-31 | 2011-07-06 | 北京热景生物技术有限公司 | Immunochromatography rapid kit and production method thereof |
| CN102692512A (en) * | 2012-06-26 | 2012-09-26 | 南京基蛋生物科技有限公司 | Double-antibody sandwich rapid detection kit of N-terminal pro-brain natriuretic peptide (NT-proBNP) |
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