CN202757936U - Colloidal gold immunochromatography assay test strip - Google Patents
Colloidal gold immunochromatography assay test strip Download PDFInfo
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- CN202757936U CN202757936U CN 201220187339 CN201220187339U CN202757936U CN 202757936 U CN202757936 U CN 202757936U CN 201220187339 CN201220187339 CN 201220187339 CN 201220187339 U CN201220187339 U CN 201220187339U CN 202757936 U CN202757936 U CN 202757936U
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- colloidal gold
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- test paper
- paper strip
- chromatography test
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Abstract
The utility model relates to the field of clinical medical examination, and discloses a colloidal gold immunochromatography assay test strip. The colloidal gold immunochromatography assay test strip comprises a gold label film adsorbing the colloidal gold protein, a coating film, a sample pad, water absorbing pads and a bottom liner, wherein the coating film is adhered onto the upper middle of the bottom liner, the water absorbing pads are adhered to two ends of the bottom liner, the gold label film adsorbing the colloidal gold protein is adhered to the upper middle of the coating film, and the sample pad is arranged on the gold label film adsorbing the colloidal gold protein. Since the coating film, the gold label film adsorbing the colloidal gold protein and the sample pad are arranged in the middle of the bottom liner layer by layer, and the water absorbing pads are arranged at two ends of the bottom liner, the colloidal gold immunochromatography assay test strip can realize two-way chromatography, stability in colloidal gold immunochromatography can be enhanced, and product quality is improved. Further, the surface of the coating film is coated with antigen protein or antibody protein, so that test types of antigen and antibody are expanded, and the application range of the colloidal gold immunochromatography assay test strip is widened.
Description
Technical field
The utility model relates to the clinical medical inspection field, particularly a kind of colloidal gold immuno-chromatography test paper strip.
Background technology
Gold chloride (HAuCl
4) under the reductive agent effect, can be grouped to a certain size gold grain, form electronegative hydrophobic sol solution, and because electrostatic interaction can condense into stable colloidal state, therefore be commonly called collaurum.The positive charge group of colloid gold particle surface negative charge and protein can form strong bonded because of Electrostatic Absorption; because colloid gold particle has the characteristic of high electron density; when therefore gold mark albumen is assembled in a large number at corresponding part place, at the visible pitchy particle of microscopically or naked eyes red color visible or pink spot.In the clinical medical inspection field, usually with collaurum as indicator, antigen, haptens and antibody are detected.Colloidal gold immuno-chromatography test paper strip is exactly wherein modal a kind of, and is quick because of it, easy to be higher with sensitivity, and is widely applied in the clinical examination field.Wherein, the burst size of collaurum, chromatography speed and homogeneity have determined the product quality of colloidal gold immuno-chromatography test paper strip, the sample pad of colloidal gold strip product in the market is little with the polyester film contact area that contains collaurum, cause collaurum to discharge slow and inhomogeneous, in addition, this series products mostly is single antigen or antibody test, and application is restricted.Based on the problems referred to above, the utility model has changed the combined method of common colloidal gold strip, has improved product quality, has enlarged the range of application of collaurum.
The utility model content
The purpose of this utility model is to provide a kind of colloidal gold immuno-chromatography test paper strip, so that colloidal gold immuno-chromatography test paper strip can two-dimensional chromatography, thereby can increase the stability of colloidal gold chromatographic, has improved product quality.
For solving the problems of the technologies described above, embodiment of the present utility model provides a kind of colloidal gold immuno-chromatography test paper strip, comprises: gold mark film 1, coated film 2, sample pad 3, adsorptive pads 4 and the end liner 5 of absorption collaurum albumen;
Coated film 2 is pasted at middle part above the described end liner 5, and adsorptive pads 4 is pasted at end liner 5 two ends;
Gold mark film 1 is pasted at middle part above the described coated film 2;
Described sample pad 3 is arranged on the described gold mark film 1.
Wherein, the bottom of described gold mark film 1 is the dacron film, at this dacron film adsorption collaurum albumen is arranged.The bottom of coated film 2 is the dacron film, at this dacron film pan coating antigen or antibody protein is arranged.
The utility model embodiment in terms of existing technologies, by the middle part that coated film, the gold mark film that adsorbs collaurum albumen and sample pad layering are arranged at end liner, and adsorptive pads is arranged at the two ends of end liner, so that colloidal gold immuno-chromatography test paper strip can two-dimensional chromatography, thereby can increase the stability of colloidal gold chromatographic, improve product quality.
In addition, described sample pad 3 is covered on the gold mark film 1 fully.Sample pad is fully contacted with gold mark film, discharge rapidly and equably thereby make collaurum, further improved product quality.
In addition, the envelope antigen on the described coated film 2 or antibody protein 6 are positioned at the two ends of coated film, further increase the stability of colloidal gold chromatographic, have improved product quality.
In addition, an end of coated film 2 is coated with antigen protein, and the other end is coated with antibody protein.Increase the antigen and antibody kind, enlarged the range of application of colloidal gold strip.
Description of drawings
Fig. 1 is the cross-sectional configuration schematic diagram according to the colloidal gold immuno-chromatography test paper strip of the utility model the first embodiment;
Fig. 2 is the plan structure schematic diagram according to the colloidal gold immuno-chromatography test paper strip of the utility model the 3rd embodiment.
Embodiment
For making the purpose of this utility model, technical scheme and advantage clearer, below in conjunction with accompanying drawing embodiment of the present utility model is explained in detail.Yet, persons of ordinary skill in the art may appreciate that in the utility model embodiment, in order to make the reader understand the application better many ins and outs have been proposed.But, even without these ins and outs with based on many variations and the modification of following embodiment, also can realize each claim of the application technical scheme required for protection.
The first embodiment of the present utility model relates to a kind of colloidal gold immuno-chromatography test paper strip, comprises: gold mark film 1, coated film 2, sample pad 3, adsorptive pads 4 and the end liner 5 of absorption collaurum albumen, as shown in Figure 1.Coated film 2 is pasted at middle part above the end liner 5, and adsorptive pads 4 is pasted at end liner 5 two ends; Gold mark film 1 is pasted at middle part above the coated film 2; Sample pad 3 is arranged on the gold mark film 1.And compress adsorptive pads 4 and sample pad 3 by the shell of colloidal gold immuno-chromatography test paper strip.
Wherein, the bottom of gold mark film 1 is the dacron film, at this dacron film adsorption collaurum albumen is arranged; The bottom of coated film 2 is the dacron film, at this dacron film pan coating antigen or antibody protein is arranged; The material of sample pad 3 is dacron films; The material of adsorptive pads 4 is the papery complex fiber material; The material of end liner 5 is glass fibre membrane.
The preparation method of the colloidal gold immuno-chromatography test paper strip of present embodiment is as follows:
Step 1, the dacron film of preparation absorption collaurum albumen, i.e. gold mark film 1;
The using method of the colloidal gold immuno-chromatography test paper strip of present embodiment is as follows:
Sample solution is dripped on sample pad 3, sample solution is through the permeation filtration of sample pad 3, be diffused on the gold mark film 1, because the effect of pressing of gold mark film 1 and coated film 2, and the position of gold mark film 2 is placed on middle, sample solution and collaurum albumen are uniformly to the diffusion of both sides chromatography, namely to adsorptive pads 4 diffusions.
In terms of existing technologies, the middle part of present embodiment by coated film, the gold mark film that adsorbs collaurum albumen and sample pad layering are arranged at end liner, and adsorptive pads is arranged at the two ends of end liner, so that colloidal gold immuno-chromatography test paper strip can two-dimensional chromatography, thereby can increase the stability of colloidal gold chromatographic, improve product quality.
The utility model the second embodiment relates to a kind of colloidal gold immuno-chromatography test paper strip.The second embodiment has been done further improvement on the first embodiment basis, and its main improvements are: in the utility model the second embodiment, sample pad 3 is covered on the gold mark film 1 of absorption collaurum albumen fully.Sample pad is fully contacted with gold mark film, discharge rapidly and equably thereby make collaurum, further improved product quality.
The utility model the 3rd embodiment relates to a kind of colloidal gold immuno-chromatography test paper strip.The 3rd embodiment has been done further improvement on the first embodiment basis, its main improvements are: in the utility model the 3rd embodiment, envelope antigen on the coated film 2 or antibody protein 6 are positioned at the two ends of coated film, further increase the stability of colloidal gold chromatographic, improved product quality.
In addition, it will be understood by those skilled in the art that antigen or the antibody protein on the coated film can be ribbon, and can respectively arrange one or more at two ends, as shown in Figure 2.
The 4th embodiment of the present utility model relates to a kind of colloidal gold immuno-chromatography test paper strip.The 4th embodiment has been done further improvement on the 3rd embodiment basis, and its main improvements are: in the utility model the 4th embodiment, an end of coated film 2 is coated with antigen protein, and the other end is coated with antibody protein.Increase the antigen and antibody kind, enlarged the range of application of colloidal gold strip.
Persons of ordinary skill in the art may appreciate that the respective embodiments described above are to realize specific embodiment of the utility model, and in actual applications, can do various changes to it in the form and details, and do not depart from spirit and scope of the present utility model.
Claims (9)
1. a colloidal gold immuno-chromatography test paper strip is characterized in that, comprises: gold mark film (1), coated film (2), sample pad (3), adsorptive pads (4) and the end liner (5) of absorption collaurum albumen;
Described coated film (2) is pasted at middle part above the described end liner (5), and described adsorptive pads (4) is pasted at described end liner (5) two ends;
Described gold mark film (1) is pasted at middle part above the described coated film (2);
Described sample pad (3) is arranged on the described gold mark film (1).
2. colloidal gold immuno-chromatography test paper strip according to claim 1 is characterized in that, described sample pad (3) is the dacron film.
3. colloidal gold immuno-chromatography test paper strip according to claim 1 is characterized in that, the bottom of described gold mark film (1) is the dacron film, at this dacron film adsorption collaurum albumen is arranged.
4. colloidal gold immuno-chromatography test paper strip according to claim 1 is characterized in that, the bottom of described coated film (2) is the dacron film, at this dacron film pan coating antigen or antibody protein is arranged.
5. colloidal gold immuno-chromatography test paper strip according to claim 1 is characterized in that, described sample pad (3) is covered on the described gold mark film (1) fully.
6. colloidal gold immuno-chromatography test paper strip according to claim 4 is characterized in that, the two ends that the envelope antigen on the described coated film (2) or antibody protein are positioned at coated film.
7. colloidal gold immuno-chromatography test paper strip according to claim 6 is characterized in that, antigen or antibody protein on the described coated film (2) are ribbon.
8. colloidal gold immuno-chromatography test paper strip according to claim 7 is characterized in that, there be respectively at least one described antigen or antibody protein in the two ends in described coated film (2).
9. colloidal gold immuno-chromatography test paper strip according to claim 6 is characterized in that, an end of described coated film (2) is coated with antigen protein, and the other end is coated with antibody protein.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220187339 CN202757936U (en) | 2012-04-27 | 2012-04-27 | Colloidal gold immunochromatography assay test strip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220187339 CN202757936U (en) | 2012-04-27 | 2012-04-27 | Colloidal gold immunochromatography assay test strip |
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| Publication Number | Publication Date |
|---|---|
| CN202757936U true CN202757936U (en) | 2013-02-27 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220187339 Expired - Fee Related CN202757936U (en) | 2012-04-27 | 2012-04-27 | Colloidal gold immunochromatography assay test strip |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109298182A (en) * | 2017-07-24 | 2019-02-01 | 北京健平九星生物医药科技有限公司 | A kind of preparation method and fluorescence immune chromatography card of fluorescence immune chromatography card |
| CN110095594A (en) * | 2019-05-27 | 2019-08-06 | 武汉上成生物科技有限公司 | A kind of fluorescence immune chromatography kit of rapid quantitative detection vomitus toxin |
-
2012
- 2012-04-27 CN CN 201220187339 patent/CN202757936U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109298182A (en) * | 2017-07-24 | 2019-02-01 | 北京健平九星生物医药科技有限公司 | A kind of preparation method and fluorescence immune chromatography card of fluorescence immune chromatography card |
| CN110095594A (en) * | 2019-05-27 | 2019-08-06 | 武汉上成生物科技有限公司 | A kind of fluorescence immune chromatography kit of rapid quantitative detection vomitus toxin |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20160718 Address after: 201815 Shanghai City, Jiading District Xing Xian Lu No. 1180 building 7 room 307 3 Patentee after: SHANGHAI SHUANGRU BIOTECH CO., LTD. Address before: 201112, Shanghai, Minhang District, Union Road 1188, 7, 3, C301 room Patentee before: Shanghai Tianru Biological Science & Technology Co., Ltd. |
|
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130227 Termination date: 20200427 |