CN202720231U - Test strip for testing specimen - Google Patents
Test strip for testing specimen Download PDFInfo
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- CN202720231U CN202720231U CN 201220316202 CN201220316202U CN202720231U CN 202720231 U CN202720231 U CN 202720231U CN 201220316202 CN201220316202 CN 201220316202 CN 201220316202 U CN201220316202 U CN 201220316202U CN 202720231 U CN202720231 U CN 202720231U
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Abstract
The utility model relates to a device for testing a specimen, in particular to a test strip for testing the specimen. The test strip for testing the specimen comprises a gold-marking pad, a test pad, and a gold-marking buffer pad. According to the test strip, due to the gold-marking buffer pad, the reaction between the specimen and an anti-body is fuller; meanwhile, outer layered non-setting adhesive and the inner layered gold-marking pad are separated, so that the problem that the heights on both sides of the gold-marking pad are different does not exist or the phenomenon that the heights of both sides of the gold-marking pad are different is reduced during the production process of the test strip, the speed for the specimen to flow through the gold-marking pad is enabled to be the same, the components of an analyzed substance in the specimen and the antibody with the gold-marking pad can be integrated. Finally, the phenomena of the breakage of a test structural line and different colors are effectively reduced.
Description
Technical field
The utility model relates to the device that detects sample, especially for the test strips that detects sample.
Background technology
[0002] this technology of utilizing immune association reaction principle to detect whether to have analyte in the sample extensively is used in every field.Can come with the analyte that it detects various biological specimens (saliva, blood, urine, serum, sweat etc.) monitoring of diseases and human health status (early pregnancy, tumour, infectious disease, drugs etc.).The cardinal principle of this detection technique is to be based upon the performance that has specific bond between the immune molecule, for example antibody and antigen, haptens/antibody, biotin and antibiotin etc.In addition, a lot of such detections can be finished at solid dielectric, the cross flow reagent strip of for example commonly using, and in glass or the plastics porous disc, device for immunochromatography etc.Usually, can be again in conjunction with some solid particles or chemical substance on immune specific bond molecule, can come by naked eyes or other instrument and equipments so qualitative, the quantitative or semiquantitative testing result that draws.This solid particle can be colored colloidal solid (latex or gold grain), and this chemical substance can be the material with chromophoric group, and these materials can send specific wavelength and show testing result under other suitable conditions.
Utilize detection reagent strip or the device of these principles can find in the prior art, for example the reagent strip described of the following patent or the device that contains reagent strip: US 4857453; US 5073484; US 5119831; US 5185127; US 5275785; US 5416000; US 5504013; US 5602040; US 5622871; US 5654162; US 5656503; US 5686315; US 5766961; US 5770460; US 5916815; US 5976895; US 6248598; US 6140136; US 6187269; US 6187598; US 6228660; US 6235241; US 6306642; US 6352862; US 6372515; US 6379620; With US 6403383.
Immune detection generally includes two kinds of principles, and Sanming City therapy and competition law are wherein common with the haptens small-molecule substance that competing method detects in the sample.Utilize method that competition law detects and reagent and pick-up unit in US Patent No. 4235601; US4442204, US5208535 has detailed description in the US5229073.These devices all are to be described on the surveyed area or the result reads when not having change color on the zone or not having the color lines to occur, testing result is judged to the positive, expression detects sample may exist analyte, on the contrary, when surveyed area or result read when occurring change color on the zone or having the color lines to occur, testing result is judged to feminine gender, and expression detects in the sample may not exist analyte.
Therefore, utilize the control line of surveyed area on the test strips and/or the demonstration of testing result line to detect whether there is certain analyte in the sample.Yet, these test strips are actual when making to detect in the sample analyte, there are some problems: because test strips is production in enormous quantities, in the generative process of each test strips, need to cut, and after the cutting, the adhesive sticker on test strips surface is because of the stressed difference on blade both sides, the both sides adhesive sticker and the sample pad that cause test strips, the cohesive forces such as gold mark pad are different, and generally speaking, one side of test strips (for example left side) adhesive sticker is very tight with gold mark pad bonding, then because of stressed difference, it is very loose to bond for another side (such as the right).Like this, when detecting sample, gold mark pad causes the flow velocity of sample on gold mark pad different because thickness is different, cause being combined inhomogeneous with the analyte of golden target antibody in sample, finally can cause demonstration-detection line or the control line of testing result to produce broken string, namely lines show incomplete phenomenon.Also have the difference phenomenon of testing result when simultaneously, also existing same sample to use with a collection of ELISA test strip.Thereby, have a strong impact on reading and judging of testing result.
Summary of the invention
[0003] in order to address these problems, the utility model provides the test strips with different structure, the test strips of this different structure can make sample before arriving detecting pad and with the even combination of golden target antibody, further, this improved structure can make the outer adhesive sticker of test strips not be connected with gold mark pad, thereby overcome the different problem of gold mark pad both sides height, having solved test result has the problem that breaks or the result difference phenomenon is arranged.
A kind of test strips for detection of sample provided by the utility model comprises gold mark pad and detecting pad, and detecting pad is positioned at the downstream of gold mark pad, and, also comprise gold mark cushion pad on this test strips.Further in the mode, gold mark cushion pad is between gold mark pad and detecting pad.After having increased a gold mark cushion pad between gold mark pad and the detecting pad, after sample added gold mark pad, analyte in the sample and pre-service being combined with golden target antibody on gold mark pad in advance, after flowing through gold mark pad, enter into gold mark cushion pad, on gold mark cushion pad, in the sample analyte with further distribute and combination with golden target antibody, thereby it is more even that analyte is combined with antibody, also is that the gold mark that carries on each analyte is more even; That is to say that the analyte in the sample lengthens with antibody and the gold mark label cohesive process that the gold mark fills up, can make reaction more abundant, also can carry out the result when testing thereby enter into detecting pad at next step so that gold mark carries more evenly, the testing result line shows more even.Thereby broken string and the even problem of irregular colour of test result lines have further been improved.
In the embodiment, between gold mark pad and the detecting pad spacing is arranged, gold mark cushion pad connects gold mark pad and detecting pad, and the two is connected.Between gold mark pad and the detecting pad spacing is arranged, only be communicated with by gold mark cushion pad, can guarantee further that the analyte in the sample flow on the detecting pad again after being with golden target antibody to be combined more fully.Like this, so that the testing result line shows more even.
More specifically in the mode, gold mark cushion pad one end is connected with an end of gold mark pad, and the other end is connected with detecting pad.Sample flow into an end of the gold mark cushion pad that is attached thereto from gold mark pad, advances the buffering of gold mark cushion pad, namely analyte be with golden target antibody fully in conjunction with after, the other end by gold mark cushion pad flow on the detecting pad again.
In another embodiment, the end that gold mark cushion pad is connected with gold mark pad is positioned at the top of gold mark pad.
In other concrete embodiments, test strips also comprises outer adhesive sticker.Preferred embodiment, outer adhesive sticker is separated with gold mark pad.In the embodiment, outer adhesive sticker covers detecting pad and is positioned on the gold mark cushion pad of gold mark pad top; Gold mark cushion pad is separated outer adhesive sticker and gold mark pad.
Gold marks cushion pad so that outer adhesive sticker is direct and gold mark pad is not connected, thereby the viscosity of adhesive sticker just can not have influence on golden mark pad.Therefore, in the cutting manufacturing process of test strips, gold mark pad can not cause because of the both sides unbalance stress of test strips each several part uneven thickness (particularly the difference in thickness of the gold mark pad of two of test strips sides is larger).And gold mark mat thickness all for the moment, also flow velocity is even for the fluid that the gold mark of flowing through fills up, also even with the reaction of gold mark label, thereby also even when next step testing result shows, avoided the test result on the same test strips to show that the testing result Show Color of broken string phenomenon and different test strips has the aberration phenomenon.
In the embodiment, test strips also comprises sample pad, and this sample pad is positioned at the upstream of gold mark pad, and sample pad is between outer adhesive sticker and gold mark pad.In the embodiment, sample pad is connected with the other end that gold mark pad is not connected with gold mark cushion pad.More specifically, sample pad is positioned at the top of gold mark pad.Like this, sample pad separates gold mark pad and outer adhesive sticker jointly with gold mark cushion pad, so that test strips is in manufacturing process, gold mark pad can be different because of the cohesive force thickness of outer adhesive sticker.
In another embodiment, gold mark cushion pad can be the extension of sample pad.
One preferred embodiment in, at regular intervals between gold mark cushion pad and the sample pad, this spacing makes sample pad and gold mark cushion pad not join or directly be communicated with.This spacing separates sample pad and gold mark cushion pad, and the two directly is not communicated with, and sample can only flow into first on the gold mark pad on sample pad, flows in turn on the gold mark cushion pad from gold mark pad again.Like this, can effectively avoid all or part of gold mark pad of not flowing through of sample directly to flow into gold mark cushion pad from sample pad.And when sample does not flow through gold mark pad, just can't be combined with being positioned at the upper antibody of gold mark pad and gold mark label, like this, can make the ELISA test strip failure.
In another embodiment, there is the adhesive sticker overlay film outer adhesive sticker inboard in gold mark pad corresponding position.Adhesive sticker or transparent adhesive tape overlay film are not connected outer adhesive sticker and gold mark pad for these.Because the internal layer of the outer adhesive sticker of test strips has viscosity, when it covered sample pad and gold mark pad when upper, the upper strata bonding of adhesive sticker internal layer and sample pad and gold mark pad, and in test strips manufacturing process, in cutting process, the stressed difference on blade both sides, cause the adhesive sticker of same test strips dual-side different from the cohesive force of the parts of bottom, and, effect because of blade, the stickum of part adhesive sticker also can be brought to the foot of test strips, thereby, can compress and be positioned at the middle sample pad of test strips and gold mark pad.Simultaneously, fluffy because of gold mark pad material, cause the gold mark pad on the test strips different because of position difference thickness everywhere easilier.So, in the adhesive sticker position corresponding with gold mark pad, at the sticking inner film coating of the tool of adhesive sticker, so that this place does not have viscosity, also separated simultaneously outer adhesive sticker and gold mark pad, just can avoid the generation of the problems referred to above.
In some embodiments, the material of gold mark cushion pad is dacron film or nitrocellulose filter.
In another embodiment, increase the sample pad height, so that gold mark pad increases with distance between the outer adhesive sticker, thereby so that outer adhesive sticker is marked to fill up with gold does not want to be connected.
In another concrete embodiment, can increase the distance between gold mark pad and the detection line.Like this, there are time enough and distance to come so that the analyte in the sample mixes with gold mark pad, thereby, can behind surveyed area and corresponding antibody response, obtain uniform testing result line.
Beneficial effect
Test strips of the present utility model is because there being gold mark cushion pad, so that sample and antibody response are more abundant; The gold mark pad of outer adhesive sticker and internal layer is separated, thereby so that test strips can not make in process of production the both sides height difference of gold mark pad or reduce the different phenomenon of golden mark pad both sides height, it is identical to have guaranteed that sample flow is crossed the speed in when pad gold mark so that the analyte in the sample with can be combined by composition with golden target antibody.Finally, effectively reduce broken string and the color distortion phenomenon of test structure line.
Description of drawings
Fig. 1 is the structural representation of common test strips
Fig. 2 is the structural representation of an improved test strips of the present utility model
Fig. 3 is the structural representation of another improved test strips of the present utility model
The structural representation of Fig. 4 another improved test strips of the present utility model
The structural representation of Fig. 5 another improved test strips of the present utility model
Description of reference numerals:
Test strips 100,200,300,400,500; Sample pad 120; Outer adhesive sticker 110; Gold mark pad 130; Detecting pad 140; Adsorptive pads 150; Back sheet 160; Test result C line 142; Test result T line 141; Gold mark cushion pad 170; One end 172 of gold mark cushion pad; The other end 171 of gold mark cushion pad; Spacing 180 between gold mark cushion pad and the sample pad; Adhesive sticker inner film coating 190; Spacing 181 between gold mark pad and the detecting pad.
Embodiment
The below is described further structure or these the employed technical terms that the utility model relates to.
Test strips
Applying to reagent strip 100 of the present utility model can be usually said horizontal effluent reagent strip, and the concrete structure of these reagent strips and detection principle are the known technology of persons skilled in the art in the prior art.Common reagent strip, comprise the sample collection zone, marked region, surveyed area and suction zone, the sample collection zone comprises sample pad, marked region comprises gold mark pad, suction zone can comprise adsorptive pads, wherein be positioned at and comprise on the surveyed area on the detecting pad and can detect the necessary chemical substance that whether contains analyte, when detect finish after, expression result's detection line and control line can appear at surveyed area.General commonly used detection reagent strip is the nitrocellulose filter reagent strip, and namely surveyed area comprises nitrocellulose filter (NC film), nitrocellulose filter fixedly the specific bond molecule show the result of detection, can also be cellulose acetate membrane or nylon membrane etc.Usually the width of test strips is between 3mm-6mm, and in the specific embodiment, the width of test strips is 4mm.
In the following detailed description, the subsidiary reference word of legend is a part here, and it illustrates to illustrate the mode that the utility model can actable specific concrete scheme.We do not get rid of the utility model and can also carry out other concrete scheme and changing structure of the present utility model in the situation of usable range of the present utility model.
As shown in Figure 1, common test strips 100 is by outer adhesive sticker 110, sample pad 120, and gold mark pad 130 and detecting pad 140, the sheet material 160 of adsorptive pads 150 and the bottom consists of.Usually, when test strips is made, place first detecting pad 140 on back sheet 160, and be connected with gold mark pad 130 in an end (upstream) top of detecting pad, the other end (downstream) is connected with adsorptive pads 150.Above gold mark pad 130, be connected with sample pad 120.Then be covered with the non-drying glue layer 110 of the superiors above sample pad 120, this non-drying glue layer 110 fills up 130 for the protection of the sample pad 120 that is positioned at its below and gold mark, and is used for indicating some signs.These adhesive sticker 110 internal layers have viscosity, and bonding is positioned at the sample pad 120 of its below, gold mark pad 130 and detecting pad 140.In practical operation, sample is added on the sample pad 120, such as, sample pad 120 parts of test strips are entered in the sample solution, sample then is adsorbed onto in the sample pad 120, and along the test strips cross flow, sample reacts accordingly through the gold mark pad 130 concurrent lifes that contact with sample pad 120 first, thereafter the antibody of analyte and the upper reaction of gold mark pad continues to flow forward together in the sample, arrive on the detecting pad 140, at control zone 142 and the detection zone 141 and the related substances reaction that is located thereon of detecting pad, thereby manifest testing result.And test strips is enterprising cross detecting pad after also unnecessary liquid continue to flow to forward, the downstream of arrival detecting pad is absorbed by adsorptive pads 150.Certainly, common test strips 100 also can only be made of gold mark pad 130 and detecting pad 140, and sample also can directly be added on the gold mark pad, carries out finishing detection in conjunction with rear flow detection pad with antibody on the gold mark pad.
Usually, can there be the phenomenon of the as a result display line irregular colour of testing result display line broken string or a plurality of test strips in conventional test strips, analyzing reason, is that the carry golden target antibody of analyte on gold mark pad that arrives the detecting pad of test strips is combined inhomogeneous causing.Simultaneously, in the process of common making test strips 100, the stressed difference of test strips dual-side, cause the outer adhesive sticker 110 of test strips different with NC film cohesive force from sample pad and the gold mark pad of lower floor at two sides, because of sample pad 120 and gold mark pad 130 structures fluffy, thereby cause sample pad 120 different at the thickness on the both sides of test strips from gold mark pad 130, that is to say, it is different to be positioned on the gold mark pad 130 of same test strips thickness, thereby cause the flow velocity of the liquid that flows through from gold mark pad 130 different, be combined also inhomogeneous with golden target, behind the control area 142 and surveyed area 141 that arrive with inhomogeneous golden target sample on the detecting pad 140, with be blocked behind this regional antigen or the antibody response, thereby demonstrate gold mark color, but antibody or the antigen of gold mark color before therefore carries the inhomogeneous irregular colour that causes, can produce namely that shade on broken string or the line differs and different test strips on the detection line color distortion very large.
Therefore, address this is that, the analyte that need to arrive detecting pad 140 is combined evenly with being with golden target antibody, could be detected fill up on 140 with the antigen of surveyed area or antibody response after demonstrate uniform testing result line 142.And, need simultaneously so that outer adhesive sticker 110 does not contact with gold mark pad 130, thereby gold mark pad 130 can be because of not different from the different thickness of cohesive force of adhesive sticker 110.Among the embodiment of the present utility model, increase a gold mark cushion pad 170 in common test strips 100, as shown in Figure 2, this cushion pad 170 is positioned at after the gold mark pad 130, before the detecting pad 140, and this cushion pad 170 connects and is communicated with gold mark pad 130 and detecting pad 140.As shown in Figure 2, gold mark cushion pad 170 1 ends 171 are positioned on the gold mark pad 130, are connected with gold mark pad, certainly, gold mark cushion pad 170 also can be positioned under the gold mark pad 130 and be attached thereto, and the other end 172 of gold mark cushion pad 170 is positioned on the detecting pad 140 and is attached thereto logical.In this embodiment, gold mark pad 130 directly contact with detecting pad 140, and there is spacing 181 centre, and the golden cushion pad 170 of marking plays the effect of bridge between gold mark pad and detecting pad.After sample arrives gold mark pad 130 by sample pad 120, analyte in the sample (antigen or antibody) mixes also combination with the golden target antibody that carries that the gold mark fills up on 130, at this moment, thickness difference because of gold mark pad 130, cause fast many joining golds of the sample mark of flow velocity, the few joining gold mark of sample that flow velocity is slow, then, flow into golden target sample on the gold mark cushion pad 170 in Yu Jinbiao pad 130 downstreams that put in place, when stream is marked cushion pad 170 through gold, the golden target antibody and the sample that carry distribute combination again, so that the gold mark on each sample carries evenly, again then, carry uniform golden target sample and flow to detecting pad 140, carry out next step reaction with the antibody of control area and surveyed area.Like this, at the as a result display line of control zone and surveyed area just can be even, the demonstration of having avoided broken string or a plurality of test strips as a result shade differs.
Among another embodiment, gold mark cushion pad 170 can be connected with sample pad 120, and among the embodiment, gold mark cushion pad 170 can be the extension of sample pad 120 more specifically.As shown in Figure 3.
In the preferred mode, disconnect in the middle of the sample pad of prolongation, that is to say, between sample pad 120 and the gold mark cushion pad 170 certain spacing 180 is arranged, as shown in Figure 2.Like this, can avoid sample directly to arrive gold mark cushion pad 170 without gold mark pad 130.And after if sample directly flows to the detecting pad 140 in downstream without gold mark pad 130, then analyte can't be marked combination with gold in the sample, even antigen or antibody response on the analyzed and surveyed area in the sample also can't observed results, finally cause detecting unsuccessfully.
Among another embodiment, as shown in Figure 4 and Figure 5 for adhesive sticker 110 is not contacted with gold mark pad 130, can increase one deck overlay film 190 with the inboard that gold mark pad 130 contacts at adhesive sticker 110, so that the adhesive sticker of this section does not have viscosity, thereby, the cohesive force that gold mark pad can not produce because of the viscosity of adhesive sticker causes the both sides thickness of gold mark pad different, and simultaneously, this overlay film 190 separates outer adhesive sticker 110 and gold mark pad 130 simultaneously.Like this, gold mark pad 130 just can not be different because of the whole thickness of the adhesion of adhesive sticker, and then also just so that the sample flow of the gold mark pad 130 of flowing through is identical, be combined evenly with golden target, thereby can be created in control zone and detection zone and the short-term that cause inhomogeneous by the gold mark that carries on the analyte of antigen or antibody interception or the deep mixed situation of line as a result.
Certainly, do not contact with gold mark pad 130 in order to make adhesive sticker 110, sample pad 120 is raise, cause the distance between adhesive sticker and the gold mark pad to strengthen.
Again or, in order to make sample and gold mark in conjunction with evenly, can also proper extension gold mark pad 130 and detecting pad on distance between control area and the surveyed area.Even analyte lengthens in conjunction with rear distance arriving surveyed area with the gold mark in the sample, make it when arriving surveyed area.Carry the fully evenly combination of analyte in golden target antibody and the sample.
The material of gold mark cushion pad 170 is carried golden target sample by as selection principle to guarantee to make, and in the specific embodiment, this gold mark cushion pad 170 can be selected dacron film or nitrocellulose filter.
Embodiment
For how clearer elaboration the utility model is realized, now describe with limited experiment, limited giving an example only done in these explanations under the marrow of claim of the present utility model, do not consist of the restriction to the utility model claim.
Embodiment: conventional test strips 100 and the golden contrast of marking the test strips 200 of cushion pad of interpolation
Material: 6 parts of urine collectors (containing the about 20ml of urine); 84 of the conventional test strips that our company produces are voluntarily chosen same lot number product; 84 of the test strips of the interpolation gold mark cushion pad that our company produces voluntarily are with a collection of making; Hemp standard items: 25ng/ml(-50%cutoff)
Step:
1. dispose the 25ng/ml Δ
9The positive hemp primary standard of-THC product;
2. in 6 urine collectors (containing the about 20ml of urine), add 3 milliliters of 25ng/ml Δs respectively
9Positive hemp prototype (or cannabinol) standard solution of-THC;
3. respectively the sample area of the test strips 100 of 14 routines vertically is inserted in the same urine collector, flush as boundary with the adhesive sticker surface index line of test strips take liquid level, after waiting for for 10 seconds test strips is taken out, lie on the operator's console, utilize reading test paper tape READER to read the depth degree of the surveyed area test result lines on the test strips, and convert these colouring informations to numerical value.
4. repeating step 3, respectively remaining conventional test strips 100 are inserted in remaining 5 parts of urine collectors, and namely per 14 are inserted in the same urine collector, test, and read testing result.
5. respectively 14 sample areas that add the test strips 200 of gold mark cushion pad vertically are inserted in the same urine collector, flush as boundary with the adhesive sticker surface index line of test strips take face, after waiting for for 10 seconds test strips is taken out, lie on the operator's console, utilize reading test paper tape READER to read the depth degree of the surveyed area test result lines on the test strips, and convert these colouring informations to numerical value.
6. repeating step 5, respectively remaining test strips 200 of adding gold mark cushion pad are inserted in remaining 5 parts of urine collectors, and namely per 14 are inserted in the same urine collector, test, and read testing result.
7. conventional test strip 100 is prepared as follows:
7.1. the processing of nitrocellulose filter Detection lug 140 is nitrocellulose filter (NC), at nitrocellulose filter two lines is arranged, and one is detection line 142, is positioned at the testing result control line 142 in detection line downstream.Be fixedly connected with the cannabinol molecule of BSA at detection line 141; At the fixing goat anti-rabbit igg of testing result control line 142.Fixing method can be processed automatically with automatically spraying the film handling machine, is 0.6 mg/ml processing the concentration that detects lines wherein, and dilution buffer liquid is phosphate buffer (PBS); At the fixing goat anti-rabbit igg antibody of testing result control line 142; Concentration is 1.0 mg/ml, and dilution buffer liquid is phosphate buffer (PBS).The nitrocellulose filter of handling well is placed on to dry in 45 ℃ of baking ovens gets final product.
7.2 the processing of gold mark pad 130Gold mark pad is the polyester film, and the hemp antibody good by the colloid gold particle mark is processed on the polyester film.The OD value of Treatment Solution is 60, and dilute solution is 1 times of PBS buffer solution, wherein also contains 1% BSA.On marker slip, also process the rabbit igg antibody that colloid gold label is arranged.The marker slip of handling well is placed on to dry in 37 ℃ of baking ovens gets final product.
7.3 the processing of sample pad 120Sample pad is glass fibre element film, and the solution of processing at this film is: Tween20 (1%); Cholic Acid (0.1%); Tris (0.1M).The sample blank film of handling well is placed on to dry in 37 ℃ of baking ovens gets final product.
7.4. the assembling of reagent stripAccording to order assembling shown in Figure 1, allow all parts of handling well sample pad be positioned at the upstream of gold mark pad, gold mark pad is placed adsorptive pads in the downstream of detecting pad between detecting pad and sample pad.These parts all are placed on the non-absorptive support chip.The superiors in test strips cover adhesive sticker, and at an end of test strips, adhesive sticker is with sample pad, and an end of gold mark pad and detecting pad covers; At the other end of test strips, adhesive sticker covers the other end of adsorptive pads and detecting pad.
8. have the test strip 200 of gold mark cushion pad to prepare as follows:
8.1
Nitrocellulose filter, the processing of gold mark pad 130 and sample pad 120 is identical with conventional test strips 100 disposal routes.
8.2 the processing of gold mark cushion pad.Gold mark cushion pad is the dacron film.
8.3 the assembling of test strips.The all parts of handling well is assembled according to order shown in Figure 2, allow sample pad be positioned at the upstream of gold mark pad, gold mark cushion pad is between NC film and gold mark pad, wherein, between gold mark cushion pad and the sample pad spacing 180 is arranged, place adsorptive pads in the downstream of NC film.These parts all are placed on the non-absorptive support chip.The superiors in test strips cover adhesive sticker, and at an end of test strips, adhesive sticker is with sample pad, and the gold mark fills up, and an end of gold mark cushion pad and NC film covers; At the other end of test strips, adhesive sticker covers the other end of adsorptive pads and NC film.
Test result is as follows:
Table one: the test result of common test strips 100
| Number of times | Number of iterations | Minimum value | Maximal value | Mean value | Standard deviation S D | Coefficient of variation CV(%) |
| 1 | 14 | 3.54 | 5.59 | 4.44 | 0.688 | 15.48 |
| 2 | 14 | 3.38 | 4.51 | 3.88 | 0.378 | 9.74 |
| 3 | 14 | 2.88 | 5.11 | 4.01 | 0.634 | 15.79 |
| 4 | 14 | 4.70 | 6.06 | 5.39 | 0.455 | 8.45 |
| 5 | 14 | 3.62 | 6.97 | 5.87 | 0.652 | 11.10 |
| 6 | 14 | 3.62 | 7.64 | 5.54 | 1.264 | 22.80 |
| On average | N/A | N/A | N/A | N/A | N/A | 13.89 |
Table two: with the testing result of the test strips 200 of gold mark cushion pad
| Number of times | Number of iterations | Minimum value | Maximal value | Mean value | Standard deviation S D | Coefficient of variation CV(%) |
| 1 | 14 | 3.12 | 4.52 | 3.79 | 0.400 | 10.56 |
| 2 | 14 | 3.08 | 4.90 | 3.64 | 0.586 | 16.07 |
| 3 | 14 | 3.55 | 4.72 | 4.17 | 0.361 | 8.64 |
| 4 | 14 | 5.83 | 6.91 | 6.35 | 0.366 | 5.76 |
| 5 | 14 | 3.95 | 5.66 | 4.85 | 0.524 | 10.79 |
| 6 | 14 | 5.10 | 6.52 | 5.72 | 0.427 | 7.47 |
| On average | N/A | N/A | N/A | N/A | N/A | 9.88 |
Explain:
Minimum value refers to the most shallow value of as a result display line color that 14 ELISA test strips in the same urine specimen go out.
Maximal value refers to the darkest value of as a result display line color that 14 ELISA test strips in the same urine specimen go out.
Mean value refers to the mean value of the test result of all 14 test strips.
The coefficient of variation is the ratio for standard deviation S D and mean value.
The analysis of testing result is compared:
Table two is compared with table one, and the coefficient of variation drops to 9.88, decline 28.7% from 13.89; That is to say that the color distortion of testing result data line has descended 28.7% than the color distortion of table one in the table two.
So, from top result, have less individual difference with the test strips of the gold mark cushion pad common test strips of comparing in T line signal intensity, namely have better homogeneity.In addition, in experimentation, by visual inspection, mobile more even with test strips more common test strips when sample runs plate of gold mark cushion pad, the T line is fuller evenly.
Claims (13)
1. the test strips for detection of sample comprises, gold mark pad and detecting pad, detecting pad are positioned at the downstream of gold mark pad, it is characterized in that, also comprises gold mark cushion pad on this test strips.
2. test strips according to claim 1 is characterized in that, described gold mark cushion pad is between gold mark pad and detecting pad.
3. test strips according to claim 2 is characterized in that, between gold mark pad and the detecting pad spacing is arranged, and gold mark cushion pad connects gold mark pad and detecting pad, and the two is connected.
4. test strips according to claim 3 is characterized in that, gold mark cushion pad one end is connected with an end of gold mark pad, and the other end is connected with detecting pad.
5. test strips according to claim 4 is characterized in that, the end that gold mark cushion pad is connected with gold mark pad is positioned at the top of gold mark pad.
6. one of according to claim 1-5 described test strips is characterized in that described test strips also comprises outer adhesive sticker.
7. test strips according to claim 6 is characterized in that, outer adhesive sticker covers detecting pad and is positioned on the gold mark cushion pad of gold mark pad top; Gold mark cushion pad is separated outer adhesive sticker and gold mark pad.
8. test strips according to claim 7 is characterized in that, described test strips also comprises sample pad, and this sample pad is positioned at the upstream of gold mark pad, and sample pad is between outer adhesive sticker and gold mark pad.
9. test strips according to claim 8 is characterized in that, sample pad is connected with the other end that gold mark pad is not connected with gold mark cushion pad.
10. test strips according to claim 9 is characterized in that, gold mark cushion pad can be the extension of sample pad.
11. test strips according to claim 10 is characterized in that, and is at regular intervals between gold mark cushion pad and the sample pad, this spacing makes sample pad not be connected with gold mark cushion pad.
12. test strips according to claim 11 is characterized in that, there is the adhesive sticker overlay film outer adhesive sticker inboard in gold mark pad corresponding position.
13. test strips according to claim 1 is characterized in that, the material of described gold mark cushion pad is dacron film or nitrocellulose filter.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220316202 CN202720231U (en) | 2012-06-28 | 2012-06-28 | Test strip for testing specimen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220316202 CN202720231U (en) | 2012-06-28 | 2012-06-28 | Test strip for testing specimen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202720231U true CN202720231U (en) | 2013-02-06 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220316202 Expired - Lifetime CN202720231U (en) | 2012-06-28 | 2012-06-28 | Test strip for testing specimen |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202720231U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103513030A (en) * | 2012-06-28 | 2014-01-15 | 艾博生物医药(杭州)有限公司 | Test strip for detecting samples |
| CN104977402A (en) * | 2014-04-03 | 2015-10-14 | 艾博生物医药(杭州)有限公司 | Test strip for sample detection |
| CN106771154A (en) * | 2016-11-29 | 2017-05-31 | 百奥森（江苏）食品安全科技有限公司 | The detection card and its detection method of AVM in a kind of detection tableware |
| CN107290528A (en) * | 2017-08-02 | 2017-10-24 | 东莞合安机电有限公司 | Test paper paper slip aids in turnover structure |
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2012
- 2012-06-28 CN CN 201220316202 patent/CN202720231U/en not_active Expired - Lifetime
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103513030A (en) * | 2012-06-28 | 2014-01-15 | 艾博生物医药(杭州)有限公司 | Test strip for detecting samples |
| CN104977402A (en) * | 2014-04-03 | 2015-10-14 | 艾博生物医药(杭州)有限公司 | Test strip for sample detection |
| CN109521195A (en) * | 2014-04-03 | 2019-03-26 | 艾博生物医药(杭州)有限公司 | A kind of test strips detecting sample |
| CN106771154A (en) * | 2016-11-29 | 2017-05-31 | 百奥森（江苏）食品安全科技有限公司 | The detection card and its detection method of AVM in a kind of detection tableware |
| CN107290528A (en) * | 2017-08-02 | 2017-10-24 | 东莞合安机电有限公司 | Test paper paper slip aids in turnover structure |
| CN107290528B (en) * | 2017-08-02 | 2019-01-11 | 东莞合安机电有限公司 | Test paper paper slip assists turnover structure |
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