CN109521195A - A kind of test strips detecting sample - Google Patents
A kind of test strips detecting sample Download PDFInfo
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- CN109521195A CN109521195A CN201811455381.7A CN201811455381A CN109521195A CN 109521195 A CN109521195 A CN 109521195A CN 201811455381 A CN201811455381 A CN 201811455381A CN 109521195 A CN109521195 A CN 109521195A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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Abstract
The present invention relates to the test strips of detection sample, including, label pad and detecting pad, detecting pad are located at the downstream of label pad, which is characterized in that further include sample pad in the test strips, the sample pad completely or partially covers label pad.Test strips of the invention, so that gold mark rate of release slows down, are buffered because label pad is covered by sample pad, to make analyte and the concentration proportion of gold labeling antibody in sample increase, to improve the sensitivity of test strips detection.Separate the label pad of outer layer adhesive sticker and internal layer simultaneously, so that test strips will not make the both sides height of label pad different in process of production or reduce the different phenomenon of label pad both sides height, it ensure that speed when sample flows through label pad is identical, enable the analyte in sample in conjunction with golden target antibody ingredient.Finally, the broken string and color difference phenomenon of test structure lines are effectively reduced.
Description
It is on April 3rd, 2014 that application, which is the applying date, application No. is 201410134020.8, a kind of entitled " detection
The divisional application of the application for a patent for invention of the test strips of sample ".
Technical field
The present invention relates to the devices of detection sample, especially for detecting the test strips of sample.
Background technique
This technology in sample with the presence or absence of analyte is detected using immune association reaction principle to be used extensively
In every field.The analyte of various biological samples (saliva, blood, urine, serum, sweat etc.) can be detected with it
Matter monitors the health status (early pregnancy, tumour, infectious disease, drugs etc.) of disease and the mankind.The basic original of this detection technique
Reason is built upon the performance between immune molecule with specific bond, such as antibody and antigen, haptens/antibody, biotin with
Antibiotin etc..In addition, many such detections can be completed on solid dielectric, such as common lateral flow reagent
In item, glass or plastic microtiter plates, device for immunochromatography etc..In general, can be in conjunction with one on immune specific binding molecule
A little solid particles or chemical substance, can be come in this way by naked eyes or other instruments equipment it is qualitative, quantitatively or semi-quantitatively must
Testing result out.This solid particle can be colored colloidal solid (latex or gold particle), and this chemical substance can be with
It is the substance with chromophoric group, these substances can issue specific wavelength under the conditions of other are suitable to show detection knot
Fruit.
It can be found in the prior art using the detection reagent item or device of these principles, such as the following patent
The reagent strip of description or device containing reagent strip: US4857453；US5073484；US5119831；US5185127；
US5275785；US5416000；US5504013；US5602040；US5622871；US5654162；US5656503；
US5686315；US5766961；US5770460；US5916815；US5976895；US6248598；US6140136；
US6187269；US6187598；US6228660；US6235241；US6306642；US6352862；US6372515；
US6379620；And US6403383.
Immune detection generally includes two kinds of principles, sandwich and competition law, wherein being detected in sample with competing method
Haptens small-molecule substance it is most commonly seen.Using the method and reagent and detection device of competition law detection in United States Patent (USP)
US4235601；It is described later in detail in US4442204, US5208535, US5229073.These devices are all that description is detecting
On region or when not having color change in result reading area or occur without colored line, testing result is judged to the positive,
Indicate that there may be analytes for detection sample, on the contrary, when occur in detection zone or result reading area color change or
When person has colored line to occur, testing result is judged to feminine gender, indicates that analyte may be not present in detection sample.
Therefore, it is to detect in sample using the display of the control line of detection zone in test strips and/or testing result line
It is no that there are certain analytes.However, these test strips are asked when actual use is to detect analyte in sample there are some
Topic:
After sample is added in label pad, because additive amount is concentrated, flow rate of liquid is fast, and sample is together with the marker quilt in label pad
It is cracking to be flushed on detecting pad, that is, there is a large amount of marker to be brought to detecting pad, instead drops the detection sensitivity of detecting pad
It is low.The test strips detected in particular with competition law, gradient difference between the final negative and positive as the result is shown compared with
It is small, also, in the case where the positive, it there is also simple detection line and show.This generates interference to the judgement of result, especially tries
The usually normal masses of the operator of paper slip.
Because test strips are to produce in enormous quantities, in the generating process of each test strips, cut, and after cutting,
The adhesive sticker on test strips surface leads to the both sides adhesive sticker and sample pad of test strips, label pad because the stress on blade both sides is different
Etc. cohesive forces it is different, under normal circumstances, one side (such as left side) adhesive sticker and the label pad bonding of test strips are very tight, and another side
Because stress is different if (such as the right), bond very loose.In this way, label pad leads to sample because of thickness difference when detecting sample
Flow velocity in label pad is different, causes to have golden target antibody in conjunction with the analyte in sample unevenly, eventually to lead
Display-the detection line or control line for causing testing result generate broken string, i.e., lines show incomplete phenomenon.Meanwhile there is also same
One sample is with the same as the difference phenomenon for also having testing result when a collection of test strips detection.To seriously affect the reading of testing result
It takes and judges.
Summary of the invention
In order to solve these problems, the present invention provides the test strips with different structure, the test strips of this different structure
Positive material can be made in sample in binding label, the ratio of positive material concentration and golden labelled antibody concentration will be greater than often
The test strips for advising result, keep positive material more abundant in conjunction with mark substance, finally make competing with positive material progress on detecting pad
The substance for striving combination cannot combine, and become apparent from the testing result of positive material, to keep the detection sensitivity of test strips higher；
Also, this improved structure can be such that the outer layer adhesive sticker of test strips is not connected with label pad, to overcome label pad both sides
Height different problems solve the problems, such as that test result has broken string or has result difference phenomenon.
It is provided by the present invention a kind of for detecting the test strips of sample, including, label pad and detecting pad, detecting pad are located at
The downstream of label pad, wherein further include sample pad in the test strips, the sample pad completely or partially covers label pad.Sample
After this pad partly or entirely covers label pad, after sample is added in sample pad, sample along the downward downstream of sample pad,
In moving process, label pad is touched, if there is positive analyte in sample, on positive mark's object and label pad
Marker (antibody i.e. on marker) is contacted and is combined, and the positive analyte in conjunction with after is reached further along sample pad and detected
Pad detected, because marker with the positive analyte sufficiently combined, can not again with the antigen binding on detecting pad, thus,
Marker is not had to be trapped, therefore be there will not be testing result line and shown.Because sample pad is covered in label pad, in sample pad
Sample touch the mark substance being disposed below in flow process and be combined, and sample be not by it is a large amount of quickly
It is added in label pad, therefore, marker has just achieved the effect that sustained release, and marker can have with the positive material in sample to be filled
The time divided adequately is contacted and is combined.Rather than rushed forward together as in common test strip, that is,
Release most marker at the first time, even if there are many positive material, also have little time with it is all while the label that discharges
Antibody adequately combined, cause the antibody of part tape label to flow to the antigen binding on detecting pad with detection, thus meeting
There is result line to show, namely form false negative, affects the sensitivity of detection and the judgement of testing result.It that is to say, work as sun
Property sample in conjunction with the labelled antibody of sustained release when, time that same marker is released lengthens, and is equivalent to positive material concentration
It is increased with the ratio of golden labelled antibody concentration, which is greater than ratio when the two combines in the test strips detection process of conventional result
Value, to keep the detection sensitivity of test strips higher.
More specifically, some or all of sample pad overlay marks pad refers to the part quilt in label pad with marker
Sample pad completely or partially covers.In this way, when sample flows through sample pad, the label of the part contacted in label pad with sample pad
Object, which is released in sample pad, to be contacted and combines with analyte in sample.
In one preferred embodiment, sample pad is connected with detecting pad.In this way, labelled antibody and sample in label pad
The positive material of sample is flow on detecting pad after combining along sample pad on pad, carries out the detection of next step.
In some embodiments, label pad can be connected with detecting pad.
In other preferred embodiments, there is certain spacing between label pad and detecting pad.
Label pad can connect with detecting pad, can also be not connected to.When label pad is connect with detecting pad, marker may
It can directly flow on detecting pad, influence testing result；Preferably, when label pad and detecting pad are not connected to, sample is needed
Flowing can be by mark substance (either combined be still not associated with) drive to detecting pad, in this way, just giving
Positive material and marker well-bound time and space in sample, are more advantageous to the display of testing result in this way, improve inspection
The sensitivity of survey.
In another specific preferred embodiment, sample pad separates label pad and detecting pad.That is, sample pad is located at
In spacing between label pad and detecting pad.On the other hand, sample pad is located in this spacing, is connected to label pad and detecting pad.
In another embodiment, sample pad is divided into two parts: first sample pad and the second sample pad.
Specifically, first sample pad is connected with the second sample pad；First sample pad is located at the second sample pad tie point
The upstream of label pad.More specifically, first sample pad is connected with one end of the second sample pad.
More specifically, the second sample pad overlay marks pad.That is the label pad of the second sample pad covering part or whole,
That is there is some or all of marker on the second sample pad overlay marks pad.
Preferably, the second sample pad is connected with detecting pad.More specifically, the other end and detecting pad of the second sample pad
It is connected.That is first sample pad and the detecting pad both ends that are connected to the second sample pad.
In some embodiments, test strips further include outer layer adhesive sticker.
In specific embodiment, outer layer adhesive sticker is covered on detecting pad and in the sample pad above label pad；Sample
This pad separates outer layer adhesive sticker and label pad.
Sample pad, which is covered in label pad, makes outer layer adhesive sticker is not direct to connect with label pad, thus, adhesive sticker glues
Property would not influence label pad.Therefore, in the cutting manufacturing process of test strips, label pad will not because test strips two sides by
Power unevenness leads to each section uneven thickness (difference in thickness of the especially label pad of two sides of test strips is larger).And it marks
For the moment, flowing through the fluid of label pad, also flow velocity is uniform for mat thickness, reacts also uniform with gold mark marker, thus in next step
Testing result display when it is also uniform, avoid test result display broken string phenomenon and different test strips in same test strips
Testing result display color have color difference phenomenon.
In some embodiments, the material of sample pad is polyester fiber film or nitrocellulose filter, glass fibre membrane or filter
Paper.Particularly, in a specific embodiment, first sample pad is glass fibre membrane；Second sample pad is filter paper material.
Beneficial effect
Test strips of the invention, so that gold mark rate of release slows down, are buffered, to make sample because label pad is covered by sample pad
Analyte and the concentration proportion of gold labeling antibody increase in this, to improve the sensitivity of test strips detection.Make outer layer simultaneously
The label pad of adhesive sticker and internal layer separates, so that test strips will not make the both sides height of label pad different in process of production
Or the different phenomenon of label pad both sides height is reduced, it ensure that speed when sample flows through label pad is identical, so that in sample
Analyte with can be in conjunction with ingredient with golden target antibody.Finally, the broken string and face of test structure lines are effectively reduced
Color difference phenomenon.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of common test strip；
Fig. 2 is the structural schematic diagram of an improved test strips of the invention；
Fig. 3 is the structural schematic diagram of another improved test strips of the invention；
Fig. 4 is the structural schematic diagram of another improved test strips of the invention.
Description of symbols:
Test strips 100,200,300,400；Sample pad 120；Outer layer adhesive sticker 110,210；Label pad 130,230；Detecting pad
140,240；Water absorption pad 150,250；Back sheet 160,260；Test result C line 142,244；Test result T line 141,
243；Sample pad 220；First sample pad 222；Second sample pad 221；Label pad and the spacing of detecting pad 270；The one of detecting pad
End 241；The other end 242 of detecting pad；Label pad one end 231；Sample pad one end 225.
Specific embodiment
Structure of the present invention or technical term used in these are described further below.
Test strips
Apply to the lateral effluent reagent strip that reagent strip 100 of the invention can be known as, the specific knot of these reagent strips
Structure and testing principle are technology well known to persons skilled in the art in the prior art.Common reagent strip, including sample
Collecting zone, marked region, detection zone and suction zone, sample collection region include sample pad, and marked region includes label
Pad, suction zone may include water absorption pad, wherein including that can detect whether containing being divided in the detection zone being located on detecting pad
The necessary chemical substance for analysing substance can occur indicating the detection line and control of result in detection zone after detection
Line.General common detection reagent item is nitrocellulose assay strip, i.e. detection zone includes nitrocellulose filter (NC film),
Specific binding molecule is fixed on nitrocellulose filter come show detection as a result, can also be cellulose acetate film or nylon membrane
Etc..The width of usual test strips is between 3mm-6mm, and in a specific embodiment, the width of test strips is 4mm.
In the following detailed description, the subsidiary reference word of legend is a part here, it is to illustrate this
Invention can the mode of actable specific concrete scheme illustrate.The present invention, which is not precluded, in we can also carry out others specifically
Scheme and change structure of the invention without prejudice to use scope of the invention.
As shown in Fig. 1, common test strip 100 is by outer layer adhesive sticker 110, sample pad 120, label pad 130 and detecting pad
140, the composition of sheet material 160 of water absorption pad 150 and the bottom.In general, in test strips production, in back sheet 160 first
Detecting pad 140 is placed, and is connected with label pad 130 above one end of detecting pad (upstream), the other end (downstream) is connected with suction
Water cushion 150.Sample pad 120 is connected in the top of label pad 130.Top layer is then covered in the top of sample pad 120
Non-drying glue layer 110, the non-drying glue layer 110 are used for for protecting the sample pad 120 and label pad 130 being disposed below
Indicate some marks.110 internal layer of adhesive sticker has viscosity, bonds the sample pad 120 being disposed below, label pad 130 and inspection
Survey pad 140.In actual operation, add the samples in sample pad 120, for example, by 120 part of sample pad of test strips into
Enter in sample solution, sample is then adsorbed onto sample pad 120, and along test strips lateral flow, sample first passes through and sample pad
Corresponding reaction simultaneously occurs for the label pads 130 of 120 contacts, the antibody one reacted in analyte and label pad in sample thereafter
It rises and continues flow forward, reach on detecting pad 140, in the control zone of detecting pad 142 and detection zone 141 and disposed thereon
Related substances reaction, to show testing result.And liquid continuation also extra after the enterprising detecting pad excessively of test strips is flowed forward
It arrives, reaches the downstream of detecting pad, absorbed by water absorption pad 150.Certainly, common test strip 100 can also be only by 130 He of label pad
Detecting pad 140 is constituted, and sample can also be added directly in label pad, and inspection is flowed to after being combined with the antibody in label pad
It surveys pad and completes detection.
In actual operation, common test strips 100, which can exist, reacts insensitive situation, especially for competition
The test strips of method detection, that is to say, that the negative gradient between the positive of the result of detection is poor, and the sample especially detected is
It as a result should be theoretically not no detection line, but usually have shallower detection line, or even sometimes also have ratio when positive
Obvious detection line is shown.It can make to generate puzzlement when operator's misjudgment or judging result in this way.
It is found through analysis, can generate detection line is because the antibody with marker is flow on detecting pad 140 and detection
Fixed antigen binding at line T line 141, and theoretically, in the case where sample is positive, the antibody of label should be whole
(positive material in sample is far longer than the amount of labelled antibody, and therefore, labelled antibody should in conjunction with positive material in sample
All combined), and in fact, labelled antibody there is no all and adequately in conjunction with positive material.
It solves the problems, such as this, needs to enable the labeled antibody in label pad 130 and the positive material in sample
Sufficiently or even all combine.Therefore, 200,300,400 structure of test strips of a modification of the present invention can be realized this function
Can, that is, solve this problem.As shown in Fig. 2, a novel test strips 200 equally include outer layer adhesive sticker 210, sample pad
220, label pad 230 and detecting pad 240, the sheet material 260 of water absorption pad 250 and the bottom；The difference is that the sample pad
220 are extended and are coated on label pad 230, by all or part of covering of label pad 230.It is shown in Fig. 2
It is the case where all covering.Specifically, detecting pad 240, one end 241 of detecting pad are provided in back sheet 260
On be covered with water absorption pad 250, the other end 242 is connected or is not attached to one end 231 of label pad, respectively such as Fig. 3 and Fig. 2 institute
Show.Alternatively, it is furthermore preferred that the other end 242 of detecting pad is connected with sample pad 220.More specifically, sample pad 220 is covered
The part of the label pad 230 covered is effective working portion of label pad 230, i.e. sample pad 220 covers partly or entirely
The label pad 230 for being fixed with marker.
In one specific embodiment, is handled in the label pad 230 of the improved test strips 200 of Fig. 2 and be fixed with number
The antibody for the tape label that amount is 100, and processing has the antigen of quantity 100 on the detection line T line 243 of detecting pad 240.
When being free of the positive material of detection in need in sample, after sample is added in extended sample pad 220, along sample pad 220
It is flow to the top of label pad 230, the antibody that tape label is carried after contacting swims lateral flow downwardly together, reaches detecting pad
On 240 and with the antigen binding in detection line 243 and be trapped, therefore, testing result line 243 shows the color of marker,
For example, golden target aubergine.Certainly, it there is also whether display is the control C line 244 effectively detected on detecting pad at this time.
And when containing positive material in sample, usual positive material meeting decades of times is even fixed on test strips 200 for hundreds of times
The amount of the antibody of tape label in label pad 230, we set the amount of a positive material as 1000, when 1000 positive substance
When matter flow to the lower section of label pad 230 along extended sample pad 220, because the restriction of its way of contact (passes through sample 220
Lower section and the contact of some or all of the top of label 230), so that part and positive material in the antibody of tape label object
Contact, i.e. sum are contacted and are combined with 1000 positive material for 1/10, i.e., 10 in 100 labelled antibody, then, after
The sample of continuous 1000 positive material of band, which continues to flow over, to be contacted and combines come 10 labelled antibody with next part, successively
Analogize, it is last sufficiently combine after tape label substance and sample flow detection pad 240 together, because the antibody of label is by sample
Positive material adequately combine, thus can not cannot be trapped again with the antigen binding in detection line 243.And common
The sample with positive material in traditional test strips 100, without extended sample pad or the second sample pad, in sample pad 120
Very quickly flowing (being flushed to) makes label pad 130 to in overlapped for sample pad one end label pad 130 from sample pad for this
On the abrupt release of 100 quantity reacted with 1000 positive material.Compare 10/1000 and 100/1000 ratio, it is fixed
Be so 10/1000 ratio make reaction it is more abundant.Thus, novel test strips 200 can make it is less be not in time for reaction
Labelled antibody is brought at the detection line 243 of detecting pad 240 and is trapped, thus, the lines shown at detection line are weaker, very
As for not having, so that positive testing result is shown more preferably.
In some embodiments, extended sample pad 220 can be divided into 2 sample pads: first sample for operating easily,
Pad 222 and the second sample pad 221.First sample pad 222 is for receiving sample；Second sample pad 221 is covered on label pad
On 230, react tested substance in sample adequately with labelled antibody.As shown in Fig. 3 and Fig. 4, first sample pad
222 one end is connect and disposed thereon with one end of the second sample pad 221, the second sample pad 221 by label pad 230 all
Covering.In other preferred embodiments, there is certain spacing 270, in this way, can between label pad 230 and detecting pad 240
With more fully guarantee labelled antibody with before sample contact will not with detection antigen contact, can enable labelled antibody with
Detected material in sample sufficiently combines, as shown in Fig. 4.
More specifically, there is second sample pad 221 of a part in label pad 230 and the spacing 270 of detecting pad,
The two is connected to by the second sample pad 221.As shown in Fig. 4, one end of the second sample pad 221 and first sample pad 222 connect
It connects, the other end 225 of the second sample pad is connect with the other end 242 of detecting pad, also, 221 overlay marks of the second sample pad
Pad 230 and spacing 270.
In some preferred embodiments, extended sample pad or the second sample pad also make outer layer adhesive sticker 210 and label
Pad 230 does not contact, thus label pad 230 will not due to different from the cohesive force of adhesive sticker 210 thickness it is different.Sample pad 220
Or 221 effectively separated adhesive sticker 210 and label pad 230, makes to try so as to avoid the adhesive bond because of adhesive sticker 210
The asymmetry of label pad 230 caused by paper slip 200,300,400 makes both sides stress different during machine-shaping.
The material of sample pad is to guarantee to make to carry golden target sample by for selection principle, a specific embodiment
In, sample pad 220, the material including first sample pad 222 and the second sample pad 221 is polyester fiber film or nitrocellulose
Film, glass fibre membrane or filter paper.Particularly, in a specific embodiment, first sample pad 222 is glass fibre membrane；Second
Sample pad 221 is filter paper material.
Embodiment
In order to which how the clearer elaboration present invention realizes, it is illustrated now with limited experiment, these explanations only exist
Limited citing is done under the marrow of claim of the invention, does not constitute limiting to the claimed invention.
Embodiment 1: there are two the test strips 400 of sample pad to detect big ramie product with improved tool for conventional test strips 100
(THC) comparison
Material: urine collector 6；It the conventional test strips 100 that our company voluntarily produces totally 18, chooses same lot number and produces
Product；Our company voluntarily produces tool there are two test strips 400 totally 18 of sample pad, makes with a batch；Hemp standard items:
25ng/ml。
Step:
1. configuring 25ng/ml11-nor- Δ9- THC hemp standard items；
2. adding 20 milliliters of 25ng/ml11-nor- Δs into 3 urine collectors respectively9The hemp standard solution of-THC；
Add ' negative ' specimens into other 3 urine collectors (containing urine about 20ml) respectively again；
3. the sample area of the test strips 100 of 3 routines is perpendicularly inserted into same urine collector respectively, with liquid level with
The adhesive sticker surface index line of test strips flushes as boundary, takes out test strips after waiting 10 seconds, lies on station,
The depth degree for reading the detection zone test result lines in test strips is compared using reading test paper tape standard color card, obtains number
Value；
4. repeating step 3, remaining conventional test strips 100 are inserted into remaining 5 urine collectors respectively, i.e., every 3
Root is inserted into same urine collector, is tested, and testing result is read；
5. according to 2,3,4 pairs of above step tools, there are two the test strips 400 of sample pad similarly to be tested；
6. conventional test strip 100 prepares as follows:
The reagent strip 100 as shown in Fig. 1 is used to illustrate the component and production method of reagent strip of the invention used in this experiment.
6.1. the processing of nitrocellulose filter.Detection lug 140 is nitrocellulose filter (NC), on nitrocellulose filter
There is two lines item, one is detection line 141, the testing result control line 142 positioned at detection line downstream.It is solid in detection line 141
Surely it is connected with the cannabinol molecule of BSA；Goat anti-rabbit igg is fixed on testing result control line 142.Fixed method can be with certainly
It is dynamic to spray film process machine to automatically process, wherein the concentration in processing detection lines is 0.6 mg/ml, dilution buffer
Body is phosphate buffer (PBS)；Goat anti-rabbit igg antibody is fixed on testing result control line 142；Concentration be 1.0 milligrams/
Milliliter, dilution buffer liquid are phosphate buffer (PBS).The nitrocellulose filter handled well is placed in 45 DEG C of baking ovens and is dried i.e.
It can.
The processing of 6.2 gold-labelled pads 130.Gold-labelled pad is polymer PET, is located by the hemp antibody that colloid gold particle has marked
Reason is on polymer PET.The OD value of processing solution is 60, and dilute solution is 1 times of PBS buffer solution, wherein also containing 1%
BSA.The rabbit igg antibody for having colloid gold label is also handled on marker slip.The marker slip handled well is placed in 37 DEG C of baking ovens and is dried
It is dry.
The processing of 6.3 sample pads 120.Sample pad is glass fibre element film, the solution handled on the film are as follows:
Tween20 (1%); Cholic Acid (0.1%); Tris (0.1M).The sample blank film handled well is placed on 37 DEG C of bakings
It is dried in case.
6.4. the assembling of reagent strip 100.The all parts handled well according to sequentially assembling shown in Fig. 1, sample is allowed
Pad is located at the upstream of gold-labelled pad, and gold-labelled pad places water absorption pad between detecting pad and sample pad, in the downstream of detecting pad.These
Component is all placed on the support chip of a unwetted property.Adhesive sticker is covered in the top layer of test strips, in one end of test strips,
Adhesive sticker is by sample pad, one end covering of gold-labelled pad and detecting pad；In the other end of test strips, adhesive sticker is by water absorption pad and detection
The other end of pad covers.
7. there is double sample pad test strip 400 to prepare as follows:
7.1 Nitrocellulose filter 240, gold-labelled pad 230 and, processing and the conventional test strips 100 of first sample pad 222 Processing method is identical, and the second sample pad 221 uses unprocessed filter paper。
7.2 The assembling of test strips 400.The all parts handled well according to sequentially assembling shown in Fig. 4, first is allowed
Sample pad 222 is located at 221 upstream of the second sample pad, and one end of two sample pads is connected, and gold-labelled pad 230 is located at the
The downstream of one sample pad 222, and it is located at the underface of the second sample pad 221 simultaneously, it is covered by the second sample pad 221,
In, NC film 240 is located at the downstream of gold-labelled pad 230, and has certain spacing 270, the second sample between gold-labelled pad and NC film
Pad 221 is located in this spacing and the other end 225 of the second sample pad is connected with one end 242 of NC film, in NC film
Place water absorption pad 250 in 240 downstream.These components are all placed on the support chip 260 of a unwetted property.In test strips
400 top layer covers adhesive sticker 210, in one end of test strips 400, adhesive sticker 210 by the first, second sample pad 222,
221, the covering of one end 242 of gold-labelled pad 230 and NC film；In the other end of test strips 400, adhesive sticker 210 is by water absorption pad
The other end 241 of 250 and NC film covers.
It explains:
Standard color card: the ratio color range of standard color card is between 0-10, i.e. G1-G10, wherein can show that the model of result line
It is trapped among between 7-10, i.e., in the range of the line color of testing result falls in 7-10, display testing result is feminine gender.Work as line
When item is not within the scope of this, as the testing result positive or inspection testing result are shown incorrect；
Test result is as follows.
Table one: the test result of common test strip 100
。
Table two: the testing result of the test strips 400 with double sample pad
。
The analysis of testing result is compared:
For table two compared with table one, positive sample drops to G3.5 by G4.5-G5；' negative ' specimens become G8-G8.5 from G8.Institute
With in terms of result above, improved test strips are increased than the display gradient of the testing result of conventional test strips, by G4.5-
G8 has increased to G3.5-G8.5.It is improved in this way, the sensitivity of test is opposite, operator is made to more easily determine the yin of testing result
It is positive.
Embodiment 2: using conventional test strips 100, there are two the test strips 400 of sample pad to multiple productions with improved tool
Product are detected
1. its operating procedure and embodiment 1 are essentially identical, the difference is that the positive sample of each product is respectively configured, specifically
Concentration is shown in list.
2. the production of test strips is also identical as embodiment 1, wherein the antibody fixed in the detection line of NC film with
And the antibody for the tape label fixed in label pad is corresponding with the product that needs detect.I.e. in detection line 141 or 243, Yi Jibiao
Cannabinol molecule, AMP, COC, MET, MOP, BZO, PCP, OXY, BUP of BSA are respectively fixedly connected on note pad 130 or 230.
3. experimental result:
Remarks: conventional test paper Codabar code is 1；Improved test paper Codabar code is 2.
The analysis of testing result is compared:
Improved test strips are more significant relative to the effect of the product of concentration value low (i.e. sensitivity requirement is high).Therefore, it improves
The test strips product testing high particularly suitable for sensitivity requirement, such as: hemp, buprenorphine, phencyclidine etc..
Claims (12)
1. a kind of test strips for detecting sample, including, label pad and detecting pad, detecting pad are located at the downstream of label pad, feature
It is, further includes sample pad in the test strips, the sample pad completely or partially covers label pad.
2. the test strips according to claim 1, which is characterized in that sample pad is connected with detecting pad.
3. the test strips according to claim 2, which is characterized in that the label pad can be connected with detecting pad.
4. the test strips according to claim 2, which is characterized in that between having centainly between label pad and detecting pad
Away from.
5. the test strips according to claim 4, which is characterized in that sample pad separates label pad and detecting pad.
6. test strips described in one of -5 according to claim 1, which is characterized in that sample pad is divided into two parts: first sample
Pad and the second sample pad.
7. the test strips according to claim 6, which is characterized in that first sample pad is connected with the second sample pad；The
One sample pad and the second sample pad tie point are located at the upstream of label pad.
8. the test strips according to claim 7, which is characterized in that the second sample pad overlay marks pad.
9. the test strips according to claim 8, which is characterized in that the second sample pad is connected with detecting pad.
10. test strips described in one of -9 according to claim 1, which is characterized in that the test strips further include that outer layer is not done
Glue.
11. test strips described in 0 according to claim 1, which is characterized in that outer layer adhesive sticker is covered on detecting pad and is located at mark
In the sample pad of note pad top；Sample pad separates outer layer adhesive sticker and label pad.
12. the test strips according to claim 1, which is characterized in that the material of the sample pad is polyester fiber film, glass
Glass tunica fibrosa or filter paper.
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| CN201811455381.7A CN109521195A (en) | 2014-04-03 | 2014-04-03 | A kind of test strips detecting sample |
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| CN201811455381.7A CN109521195A (en) | 2014-04-03 | 2014-04-03 | A kind of test strips detecting sample |
| CN201410134020.8A CN104977402A (en) | 2014-04-03 | 2014-04-03 | Test strip for sample detection |
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| CN201410134020.8A Division CN104977402A (en) | 2014-04-03 | 2014-04-03 | Test strip for sample detection |
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| CN109521195A true CN109521195A (en) | 2019-03-26 |
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| CN201811455381.7A Pending CN109521195A (en) | 2014-04-03 | 2014-04-03 | A kind of test strips detecting sample |
| CN201410134020.8A Pending CN104977402A (en) | 2014-04-03 | 2014-04-03 | Test strip for sample detection |
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| CN106370871A (en) * | 2016-09-23 | 2017-02-01 | 武汉百美生物科技有限公司 | Test paper for testing chorionic gonadotropin in human saliva and preparation method thereof |
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| CN104977402A (en) | 2015-10-14 |
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