CN203148955U - Test strip for quickly testing clonidine through immunochromatography - Google Patents
Test strip for quickly testing clonidine through immunochromatography Download PDFInfo
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- CN203148955U CN203148955U CN 201320090847 CN201320090847U CN203148955U CN 203148955 U CN203148955 U CN 203148955U CN 201320090847 CN201320090847 CN 201320090847 CN 201320090847 U CN201320090847 U CN 201320090847U CN 203148955 U CN203148955 U CN 203148955U
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- label
- clonidine
- pad
- chromatographic film
- sample pad
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Abstract
The utility model discloses a test strip for quickly testing clonidine through immunochromatography. The test strip comprises a sample pad, a gold label release pad containing western blot of a clonidine monoclonal antibody, a chromatographic membrane, absorbent paper and a PVC (polyvinyl chloride) adhesive board, wherein the sample pad, the gold label release pad, the chromatographic membrane and the absorbent paper are superimposed from the initiating end to tail end of the PVC adhesive board in sequence; a test line and a quality control line are arranged on the chromatographic membrane; and plastic membrane labels are arranged on both the sample pad and the absorbent paper. The test strip does not need special equipment, is simple and quick in operation, can complete testing within 10 minutes, is suitable to popularize and use in pig farms, feed plants and pork and product processing plants, and has the characteristics of simplicity and convenience in operation, quickness in testing, sensitivity in reaction, low price and strong specificity.
Description
Technical field
The utility model relates to a kind of test strip, relate in particular to a kind of clonidine immune chromatography rapid detecting test paper strip, the applied immunology principle, immune response by collaurum relends and helps immunochromatography technique to reach to detect the clonidine purpose, be applied to the clonidine in fast detecting pig urine, feed and pork and the goods.
Background technology
Clonidine is mainly used in the treatment clinically, severe hypertension, suffers from glaucomatous hypertension; Also be used for antimigraine, serious dysmenorrhoea; Menopause hectic fever and glaucoma.Clonidine is the adrenergic alpha-2 receptor agonist, be the stronger old depressor of a kind of drug effect, but toxic and side effect is bigger, clinical less use now.Clonidine can also improve growth of animal and improve carcass quality except as clinical will the using.Cola is decided to be peroral dosage form, has easy to usely, cheap, and pig can be put on weight after having eaten the feed that contains clonidine, increases lean meat percentage and reduces the fat meat rate, but do not influence bone and sexal maturity etc.The pork that contains clonidine is delivered to human body through food chain, accumulate a certain amount ofly, just can cause suffering a shock, and heart rate is slow, causes respiratory and circulatory failure death.Serious together group poisoning by clonidine event has taken place in China Beijing in 2009.
The common method that detects clonidine residual in feed, pig urine and the pork is the liquid chromatography-tandem mass spectrometry method.This method sensitivity is higher, but because its instrument costliness, detection time is long, and running program is more loaded down with trivial details, so applicability is not strong.
The utility model content
The technical problems to be solved in the utility model provides a kind of clonidine immune chromatography rapid detecting test paper strip, be used for detecting feed, pig urine and the fixed residual condition of pork medium coke, susceptibility height, high specificity, and do not need professional equipment, simple to operate quick, can finish detection in 10 minutes, and be fit to that basic unit cultured and the universal use at production and processing scene, that this test strips has is easy and simple to handle, test fast, the characteristics of directly perceived, cheap, the high specificity of judged result.
For solving the problems of the technologies described above, the utility model provides a kind of clonidine immune chromatography rapid detecting test paper strip, and described test strips comprises sample pad, contains golden label release pad, chromatographic film, thieving paper, the PVC haftplatte of anti-clonidine monoclonal antibody protein trace; Initiating terminal to end at the PVC haftplatte is superimposed with sample pad successively, and golden label discharges pad, chromatographic film and thieving paper; Establish detection line and nature controlling line on the chromatographic film; Be equipped with the plastic sheeting label on described sample pad and the thieving paper.
Further, described chromatographic film sticks in the middle of the PVC haftplatte.
Further, paste the plastic sheeting protection label of printing the MAX label on the described sample pad; Paste the plastic sheeting label of printed product name label on the described thieving paper.
Further, described sample pad is superimposed on golden label and discharges on the pad, and the stack width is 1mm.
Further, the detection line position is the marking of clonidine coupling protein antigen on the described chromatographic film, and the nature controlling line position is the goat anti-rabbit igg antibody protein blot.
Further, discharge pad at the initiating terminal of the chromatographic film golden label that superposes, at the end stack thieving paper of chromatographic film.
The utility model is to realize with principle of work by the following technical programs.Anti-clonidine antibody labeling is on the collaurum help of indicator particles, and label is attached on the glass fibre; Clonidine coupling protein antigen printed and fixedly on the detection line of chromatographic film (T) position, chromatographic film sticks on the PVC haftplatte, chromatographic film top is pasted golden label successively and is discharged pad to carry out the release of golden labeling antibody, pasting sample pad to handle sample, the terminal thieving paper of pasting of chromatographic film helps chromatography to finish.Paste plastic sheeting on sample pad and the thieving paper respectively with protection and discriminating.During detection, because immune response has taken place in tested sample medium coke golden labelled antibody fixed and that redissolve, printing antigen this moment when the mixed liquor chromatography is to the detection line position can't compete and be attached to golden labelled antibody, response line does not appear on the detection line, namely positive, do not have clonidine as tested sample on the contrary, form purplish red line response line with the coupled antigen reaction bonded that is printed on the film when then the antibody chromatography of golden mark is to detection zone, namely negative.In order to prove reagent validity and easily interpretation, print goat anti-rabbit igg in nature controlling line (C) position, as the Quality Control response line, no matter in the sample whether clonidine is arranged, nature controlling line C occurs all the time.
Compared with prior art, the beneficial effects of the utility model are: clonidine immune chromatography rapid detecting test paper strip of the present utility model and instrument detect has high sensitivity and high specific equally, but do not need professional equipment, simple and quick, reaction result intuitively is easy to judge, can finish detection in 10 minutes, and detect with low costly, be fit to the universal use of feed factory, pig-breeding, pork product processing industry.Producing clonidine quick detection test paper bar with immunochromatographic method, be used for detecting feed, pig urine and the fixed residual condition of pork medium coke, is that the situation of common examination requires.That this method has is easy and simple to handle, the test quick, reaction sensitivity is high, cheap, high specificity, false positive, characteristics that false negative rate is low, need not specific apparatus and professional and technical personnel, testing result is easy to observe, and testing cost is cheap, advantages such as storage and convenient transportation.Very suitable all kinds of inspection bodies and manufacturing enterprise be the illegal interpolation situation of rapid screening clonidine at the scene.
Description of drawings
Figure 1A is the side structure synoptic diagram of embodiment test strips of the present utility model;
Figure 1B is the Facad structure synoptic diagram of embodiment test strips of the present utility model;
Fig. 2 is the testing result synoptic diagram of embodiment test strips of the present utility model; Wherein, Fig. 2 A represents the negative synoptic diagram of testing result; Fig. 2 B represents the positive synoptic diagram of testing result; It is invalid synoptic diagram that Fig. 2 C represents testing result.
Description of reference numerals is as follows among the figure:
1.PVC haftplatte, 2. chromatographic film, 3. the gold label discharges pad, 4. detection line, 5. nature controlling line, 6. sample pad, 7. thieving paper, 8. plastic sheeting label, 9. plastic sheeting protection label.
Embodiment
Below in conjunction with drawings and Examples the utility model is described in further detail.
Embodiment clonidine immune chromatography rapid detecting test paper strip
Shown in Figure 1A and Figure 1B, chromatographic film 2 is sticked on PVC haftplatte 1 centre, print detection line 4 and nature controlling line 5 in chromatographic film 2, the initiating terminal of chromatographic film 2 is pasted golden label release pad 3(gold label and is discharged an end of pad 3 and the initiating terminal stickup of chromatographic film 2, the other end and sample pad 6 are pasted), the end of chromatographic film 2 is pasted thieving paper 7.Namely initiating terminal to the end at PVC haftplatte 1 is superimposed with sample pad 6 successively, and golden label discharges pad 3, chromatographic film 2 and thieving paper 7; Pasting the plastic sheeting of printing the MAX label on the sample pad 6 protects label 9 with protection and reinforces test strips.Paste the plastic sheeting label 8 of printed product title on the thieving paper 7 with identification and reinforcing test strips.
Preparation and the detection method of the utility model clonidine immune chromatography rapid detecting test paper strip comprise the steps:
1. the detection line T(that certain density clonidine coupling bond (being the clonidine protein-coupled antigen) and goat anti-rabbit igg are printed on respectively on the nitrocellulose filter (being chromatographic film 2) is detection line 4) and nature controlling line C(be nature controlling line 5) the position.Be specially the clonidine monoclonal antibody is diluted to concentration 1mg/ml, sheep anti-mouse igg is diluted to concentration 2mg/ml, with detection line and the nature controlling line position of two kinds of solution tracks on chromatographic film, forming detection line T(is that detection line 4 and nature controlling line C(are nature controlling line 5).
2. golden label discharges the preparation of pad
Reduce four chloraurides (being gold chloride) preparations particle diameter, 40～60nm collaurum with trisodium citrate, regulate behind the pH every milliliter of collaurum and the anti-clonidine antibody of 12 μ g and be mixed into row labels and under 220nm, survey OD.OD is diluted to 3, is laid on glass fibre and absorbs oven dry, namely adhere to the anti-clonidine antibody of golden mark particle on the glass fibre.
3. the nitrocellulose filter that bag is done (chromatographic film 2), sample pad 6, golden label discharge pad 3, thieving paper 7 sticks on the PVC haftplatte 1 together successively.And be cut into the test strips of 4mm.
4. sample end and thieving paper end are pasted to identify and reinforce test strips with plastic sheeting respectively.Pasting the plastic sheeting of printing the MAX label on the sample pad 6 protects label 9 with protection and reinforces test strips.Paste the plastic sheeting label 8 of printed product title on the thieving paper 7 with identification and reinforcing test strips.
5. detection step:
A. solid sample: take by weighing a certain amount of solid sample, grind, dissolve with dilution, the centrifuging and taking supernatant namely gets sample to be checked.
Pig urine sample: directly get pig urine as detecting sample, if muddiness, then centrifuging and taking supernatant.
B. take out test strips, test strips is inserted in the solution to be checked, the solution face can not surpass the MAX position.
C.5 minute read the result in.
D. the result judges: among Fig. 2, positive findings is that red lines appear in nature controlling line C position, and detection line T position is not when showing red lines, and illustrating has clonidine in the test sample, be judged to the positive (seeing Fig. 2 B); Negative findings is that red lines appear in nature controlling line C position, and red lines also appear in detection line T position, and illustrating does not have clonidine to exist in the test sample, is judged to feminine gender (seeing Fig. 2 A); If detection line T position and nature controlling line C position all do not show red lines, then be judged to and detect invalid (seeing Fig. 2 C).
7. performance index
Sensitivity: use present embodiment, clonidine concentrations is positive more than 0.1ng/ml.
Application target: this product is used for the examination clonidine in the on-site quick screening that is used for feed, pig urine and pork, ensures pork safety.
Claims (6)
1. a clonidine immune chromatography rapid detecting test paper strip is characterized in that, described test strips comprises sample pad, contains golden label release pad, thieving paper, chromatographic film, the PVC haftplatte of anti-clonidine monoclonal antibody protein trace; Initiating terminal to end at the PVC haftplatte is superimposed with sample pad successively, and golden label discharges pad, chromatographic film and thieving paper; Described chromatographic film is provided with detection line and nature controlling line; Be equipped with the plastic sheeting label on described sample pad and the thieving paper.
2. test strips as claimed in claim 1 is characterized in that, described chromatographic film sticks in the middle of the PVC haftplatte.
3. test strips as claimed in claim 2 is characterized in that, pastes the plastic sheeting protection label of printing the MAX label on the described sample pad; Paste the plastic sheeting label of printed product name label on the described thieving paper.
4. test strips as claimed in claim 1 is characterized in that, described sample pad is superimposed on golden label and discharges on the pad, and the stack width is 1mm.
5. test strips as claimed in claim 1 is characterized in that, the detection line position is the marking of clonidine coupling protein antigen on the described chromatographic film, and the nature controlling line position is the goat anti-rabbit igg antibody protein blot.
6. test strips as claimed in claim 1 is characterized in that, discharges pad at the initiating terminal of the chromatographic film golden label that superposes, at the end stack thieving paper of chromatographic film.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320090847 CN203148955U (en) | 2013-02-28 | 2013-02-28 | Test strip for quickly testing clonidine through immunochromatography |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320090847 CN203148955U (en) | 2013-02-28 | 2013-02-28 | Test strip for quickly testing clonidine through immunochromatography |
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| Publication Number | Publication Date |
|---|---|
| CN203148955U true CN203148955U (en) | 2013-08-21 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201320090847 Expired - Fee Related CN203148955U (en) | 2013-02-28 | 2013-02-28 | Test strip for quickly testing clonidine through immunochromatography |
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| CN (1) | CN203148955U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105836240A (en) * | 2016-03-14 | 2016-08-10 | 上海快灵生物科技有限公司 | Tube breaking mechanism, sealed test tube assembly comprising tube breaking mechanism and application thereof |
| CN109521195A (en) * | 2014-04-03 | 2019-03-26 | 艾博生物医药(杭州)有限公司 | A kind of test strips detecting sample |
| CN109813897A (en) * | 2017-11-18 | 2019-05-28 | 镇江亿特生物科技发展有限公司 | Clonidine hydrochloride rapid time resolved fluorometric immunochromatographiassay assay quantitative detection test paper |
-
2013
- 2013-02-28 CN CN 201320090847 patent/CN203148955U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109521195A (en) * | 2014-04-03 | 2019-03-26 | 艾博生物医药(杭州)有限公司 | A kind of test strips detecting sample |
| CN105836240A (en) * | 2016-03-14 | 2016-08-10 | 上海快灵生物科技有限公司 | Tube breaking mechanism, sealed test tube assembly comprising tube breaking mechanism and application thereof |
| CN105836240B (en) * | 2016-03-14 | 2018-08-21 | 上海快灵生物科技有限公司 | Po Guan mechanisms, include the Po Guan mechanisms leakproofness test tube component and its application |
| CN109813897A (en) * | 2017-11-18 | 2019-05-28 | 镇江亿特生物科技发展有限公司 | Clonidine hydrochloride rapid time resolved fluorometric immunochromatographiassay assay quantitative detection test paper |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130821 Termination date: 20150228 |
|
| EXPY | Termination of patent right or utility model |