CN203148947U - Cyproheptadine immunochromatography rapid test paper card - Google Patents
Cyproheptadine immunochromatography rapid test paper card Download PDFInfo
- Publication number
- CN203148947U CN203148947U CN 201320090828 CN201320090828U CN203148947U CN 203148947 U CN203148947 U CN 203148947U CN 201320090828 CN201320090828 CN 201320090828 CN 201320090828 U CN201320090828 U CN 201320090828U CN 203148947 U CN203148947 U CN 203148947U
- Authority
- CN
- China
- Prior art keywords
- cyproheptadine
- film
- sample preparation
- chromatography
- detection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model discloses a cyproheptadine immunochromatography rapid test paper card. The paper card comprises a sample processing pad, a polyester fiber membrane, a piece of absorbent paper, a chromatography membrane and a bottom plate, wherein the sample processing pad, a gold label anti-cyproheptadine antibody polyester fiber membrane, a detection line coated with cyproheptadine antigen, a quality control line and the absorbent paper are overlapped from the start end to the tail end of the chromatography membrane in sequence; and the chromatography membrane is adhered on the bottom plate. The cyproheptadine immunochromatography rapid test paper card does not need special equipment, can be used for completing the detection in 10 minutes, is suitable for the popularization and application of breeding industry and feed mills and has the characteristics of convenience in operation, rapidness in test, sensitivity in reaction, low cost and strong specificity.
Description
Technical field
The utility model relates to a kind of detection test card, relate in particular to a kind of cyproheptadine immune chromatography rapid detecting test paper card, applied immunology principle, the immune response by collaurum relend and help immunochromatography technique to reach to detect the cyproheptadine purpose, be applied to the cyproheptadine in the fast detecting feed.
Background technology
Cyproheptadine is commonly used for the human antiallergic clinically, and the interior histamine of antagonist is to the effect of blood vessel, bronchial smooth muscle, thus the elimination allergic symptom.Cyproheptadine can also resist the slow effect that swashs skin and dopamine, and certain maincenter sedation is also arranged.Since cyproheptadine have improve a poor appetite, the effect of nervous centralis calmness, find in feed product, to add cyproheptadine in recent years.But cyproheptadine has certain harmfulness to health, and is more obvious to children's effect, toxicity symptom such as high concentration can directly cause stupor, is short of breath, general weakness, and severe patient then directly causes death of child.Therefore the Ministry of Agriculture just prohibited in 2002 and add cyproheptadine in feed.
The common method that detects cyproheptadine residual in the feed has high performance capillary electrophoresis, liquid chromatography-tandem mass spectrometry method and high performance liquid chromatography.This several method sensitivity is higher, and sense cycle is long, needs laboratory and the technical professional of specialty, and because its instrument costliness, running program is more loaded down with trivial details, and general basic unit production unit can't be popularized use, so applicability is not strong.
The utility model content
The technical problems to be solved in the utility model provides a kind of cyproheptadine immune chromatography rapid detecting test paper card, be used for detecting the interpolation situation of cyproheptadine in feed and the pig urine, be susceptibility, the best residual screening method of cyproheptadine of specificity now, and do not need Special Equipment, simple to operate quick, can finish detection in 10 minutes, and be fit to the universal use of basic unit production scene, that this test card has is easy and simple to handle, test fast, be quick on the draw, the characteristics of cheap, high specificity.
For solving the problems of the technologies described above, the utility model provides a kind of cyproheptadine immune chromatography rapid detecting test paper card, and described test card comprises sample preparation pad, dacron film, thieving paper, chromatographic film and base plate; Initiating terminal to end in chromatographic film is superimposed with the sample preparation pad successively, the anti-cyproheptadine antibody of golden mark dacron film, and bag is by cyproheptadine detection of antigens line, nature controlling line, and thieving paper; Described chromatographic film sticks on the base plate.
Further, described test card also comprises shell and lower casing, and described chromatographic film is placed between shell and the lower casing.
Further, described chromatographic film sticks in the middle of the base plate.
Further, the described shell of going up is provided with label, chromatography window, well; Described well is positioned at the top, position of sample preparation pad; Described chromatography window is positioned at the top, position of detection line and nature controlling line.
Further, an endlap of described dacron film and sample preparation pad is closed.
Further, described chromatographic film and sample preparation pad, dacron film and the superimposed place of thieving paper are covered with the material with viscosity, and chromatographic film and sample preparation pad, dacron film and thieving paper are bonded together.
The utility model is to realize with principle of work by the following technical programs.Anti-cyproheptadine antibody labeling is on help of indicator particles, and label is attached on the dacron film; With cyproheptadine comlete antigen bag by and fixedly on the detection line of chromatographic film (T), chromatographic film sticks on the base plate, the sample preparation pad is pasted to handle sample in top, terminal stickup thieving paper helps chromatography to finish.Test strips is inlaid into moulds in the card, form kit.During detection, because immune response has taken place in the golden labelled antibody of cyproheptadine antigen and redissolution in the tested sample, envelope antigen can't be competed labelled antibody when this moment, the mixed liquor chromatography was to the detection zone, detection line does not appear in the detection zone, namely positive, do not have cyproheptadine antigen as tested sample on the contrary, when then the antibody chromatography of mark is to detection zone and the antigen on the coated film in conjunction with forming purplish red line, namely negative.In order to prove reagent validity and easily interpretation, be nature controlling line at nature controlling line (C) bag by goat anti-rabbit igg respectively, so how nature controlling line C occurs all the time regardless of the result.
Compared with prior art, the beneficial effects of the utility model are: cyproheptadine immune chromatography rapid detecting test paper card of the present utility model and instrument detect has high sensitivity and high specific equally, but do not need Special Equipment, simple to operate quick, can finish detection in 10 minutes, detect with low costly, be fit to the universal use of feed factory.Produce cyproheptadine quick detection test paper card with immunochromatographic method, be used for detecting the interpolation situation of cyproheptadine in the feed, be that susceptibility, the best cyproheptadine of specificity add screening method now, that this method has is easy and simple to handle, test fast, high, cheap, the high specificity of reaction sensitivity, false positive, characteristics that false negative rate is low, need not specific apparatus and professional and technical personnel, detect fast, testing result is easy to observe, testing cost is cheap, advantages such as storage and convenient transportation.Very suitable all kinds of inspection bodies and manufacturing enterprise be the illegal interpolation situation of rapid screening cyproheptadine at the scene.
Description of drawings
Fig. 1 is the structural representation of embodiment of the present utility model.
Fig. 2 is the testing result synoptic diagram of embodiment of the present utility model; Wherein, Fig. 2 A represents the negative synoptic diagram of testing result; Fig. 2 B represents the positive synoptic diagram of testing result; It is invalid synoptic diagram that Fig. 2 C represents testing result.
Description of reference numerals is as follows among the figure:
1. go up shell, 2. lower casing, 3. base plate, 4. label, 5. the chromatography window, 6. well, 7. thieving paper, 8. the sample preparation pad, 9. the anti-cyproheptadine antibody of gold mark dacron film, 10. detection line, 11. nature controlling lines, 12.NC film (are nitrocellulose filter, nitrocellulose filter membrane, i.e. chromatographic film).
Embodiment
Below in conjunction with drawings and Examples the utility model is described in further detail.
Embodiment cyproheptadine immune chromatography rapid detecting test paper card
In Fig. 1,12(is chromatographic film with the NC film) paste in the middle of base plate 3, at the NC film 12 tested survey line 10 of bag and nature controlling lines 11, the initiating terminal of NC film 12 is pasted sample preparation pad 8, paste the anti-cyproheptadine antibody of golden mark dacron film 9 successively again, the end of NC film 12 is pasted thieving paper 7.Be placed on shell 1 with pasting complete NC film 12, cover tightly between the lower casing 2.Also be provided with label 4, chromatography window 5, well 6 on the last shell 1.Well 6 is positioned at the top, position of sample preparation pad 8, is used for application of sample; Chromatography window 5 is positioned at the top, position of detection line 10 and nature controlling line 11, the situation that is used for observing detection line 10 and nature controlling line 11; Label 4 is positioned at the optional position (for example, as shown in Figure 1, label 4 is positioned at the top, position of thieving paper 7 in the present embodiment) of shell 1, is used for the purposes of this test strips of mark etc.
Preparation and the detection method of the utility model cyproheptadine immune chromatography rapid detecting test paper card comprise the steps:
1. wrapping certain density cyproheptadine coupling bond (being cyproheptadine antigen) and goat anti-rabbit igg by the detection line T(on nitrocellulose filter (being NC film 12) respectively is detection line 10) and nature controlling line C(be nature controlling line 11) the position.For example, the cyproheptadine monoclonal antibody is diluted to concentration 1mg/ml, sheep anti-mouse igg is diluted to concentration 2mg/ml, two kinds of solution are lined detection zone and Quality Control district on the NC film, forming detection line T(is detection line 10) and nature controlling line C(be nature controlling line 11).
2. the preparation of the anti-cyproheptadine antibody of golden mark dacron film
Reduce four chloraurides (being gold chloride) preparations particle diameter, 40～60nm collaurum with trisodium citrate, regulate behind the pH every milliliter of collaurum and the anti-cyproheptadine antibody of 14 μ g and be mixed into row labels and under 286nm, survey OD.OD is diluted to 2, and to the dacron film, dries, namely adhere to the anti-cyproheptadine antibody of golden mark particle on the dacron film.
3. the nitrocellulose filter that bag is done (NC film 12), sample preparation pad 8, the anti-cyproheptadine antibody of golden mark dacron film 9, thieving paper 7 stick on the base plate 3 together successively.And be cut into the test strips of 3mm.
4. (namely be assemblied in the lower casing 2) in test strips being assemblied at the bottom of the reagent shell, the reagent casing cover that closes (namely close and go up shell 1) is assembled into cyproheptadine immune chromatography rapid detecting test paper card.
5. detection step:
A. feed sample: take by weighing a certain amount of feed, dissolve with dilution, the centrifuging and taking supernatant namely gets sample to be checked.Pig urine sample: directly get pig urine as detecting sample, if muddiness, then centrifuging and taking supernatant.
B. take out kit, keep flat on the table.
C. draw the sample to be checked that mixes with suction pipe, drip 2-3 and drip in well 6, and pick up counting.
D. in 5 minutes, read the result.
E. the result judges: among Fig. 2, positive findings is that red lines appear in nature controlling line C position, and detection line T position is not when showing red lines, and illustrating has cyproheptadine in the test sample, be judged to the positive (seeing Fig. 2 B); Negative findings is that red lines appear in nature controlling line C position, and red lines also appear in detection line T position, and illustrating does not have cyproheptadine to exist in the test sample, is judged to feminine gender (seeing Fig. 2 A); If detection line T position and nature controlling line C position all do not show red lines, then be judged to and detect invalid (seeing Fig. 2 C).
7. performance index
Sensitivity: use present embodiment, cyproheptadine concentration is positive more than 0.1ng/ml.
Application target: this product is used for the examination cyproheptadine in the on-site quick screening that is used for feed and pig urine, ensures pork safety.
Claims (6)
1. a cyproheptadine immune chromatography rapid detecting test paper card is characterized in that, described test card comprises sample preparation pad, dacron film, thieving paper, chromatographic film and base plate; Initiating terminal to end in chromatographic film is superimposed with the sample preparation pad successively, the anti-cyproheptadine antibody of golden mark dacron film, and bag is by cyproheptadine detection of antigens line, nature controlling line, and thieving paper; Described chromatographic film sticks on the base plate.
2. test card as claimed in claim 1 is characterized in that, described test card also comprises shell and lower casing, and described chromatographic film is placed between shell and the lower casing.
3. test card as claimed in claim 1 is characterized in that, described chromatographic film sticks in the middle of the base plate.
4. test card as claimed in claim 2 is characterized in that, the described shell of going up is provided with label, chromatography window, well; Described well is positioned at the top, position of sample preparation pad; Described chromatography window is positioned at the top, position of detection line and nature controlling line.
5. test card as claimed in claim 1 is characterized in that, an endlap of described dacron film and sample preparation pad is closed.
6. as claim 1 or 5 described test cards, it is characterized in that described chromatographic film and sample preparation pad, dacron film and the superimposed place of thieving paper are covered with the material with viscosity, chromatographic film and sample preparation pad, dacron film and thieving paper are bonded together.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320090828 CN203148947U (en) | 2013-02-28 | 2013-02-28 | Cyproheptadine immunochromatography rapid test paper card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320090828 CN203148947U (en) | 2013-02-28 | 2013-02-28 | Cyproheptadine immunochromatography rapid test paper card |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203148947U true CN203148947U (en) | 2013-08-21 |
Family
ID=48976463
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201320090828 Expired - Fee Related CN203148947U (en) | 2013-02-28 | 2013-02-28 | Cyproheptadine immunochromatography rapid test paper card |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203148947U (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104792989A (en) * | 2014-01-16 | 2015-07-22 | 北京维德维康生物技术有限公司 | Cyproheptadine hapten, antigen, and preparation method and applications of cyproheptadine hapten and antigen |
| CN105277706A (en) * | 2014-07-25 | 2016-01-27 | 江苏维赛科技生物发展有限公司 | Cyproheptadine hydrochloride immune colloidal gold detection card and preparation method thereof |
| CN105301245A (en) * | 2014-07-22 | 2016-02-03 | 江苏维赛科技生物发展有限公司 | Preparation method of enzyme-linked immunosorbent assay (ELISA) kit for detecting cyproheptadine hydrochloride |
| CN107688091A (en) * | 2017-09-06 | 2018-02-13 | 上海容晖生物科技有限公司 | A kind of immune quantitative test paper bar of quick detection cyproheptadine and preparation method thereof |
| CN108424880A (en) * | 2018-03-16 | 2018-08-21 | 江南大学 | A kind of hybridoma cell strain and preparation method of secretion cyproheptadine monoclonal antibody |
-
2013
- 2013-02-28 CN CN 201320090828 patent/CN203148947U/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104792989A (en) * | 2014-01-16 | 2015-07-22 | 北京维德维康生物技术有限公司 | Cyproheptadine hapten, antigen, and preparation method and applications of cyproheptadine hapten and antigen |
| CN105301245A (en) * | 2014-07-22 | 2016-02-03 | 江苏维赛科技生物发展有限公司 | Preparation method of enzyme-linked immunosorbent assay (ELISA) kit for detecting cyproheptadine hydrochloride |
| CN105277706A (en) * | 2014-07-25 | 2016-01-27 | 江苏维赛科技生物发展有限公司 | Cyproheptadine hydrochloride immune colloidal gold detection card and preparation method thereof |
| CN107688091A (en) * | 2017-09-06 | 2018-02-13 | 上海容晖生物科技有限公司 | A kind of immune quantitative test paper bar of quick detection cyproheptadine and preparation method thereof |
| CN108424880A (en) * | 2018-03-16 | 2018-08-21 | 江南大学 | A kind of hybridoma cell strain and preparation method of secretion cyproheptadine monoclonal antibody |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN203148947U (en) | Cyproheptadine immunochromatography rapid test paper card | |
| CN101261274A (en) | Method for combining colloidal gold immune leaching and immunity-chromatography for rapidly detecting medicament residue | |
| CN101625368B (en) | Casein rapid detection card and detection method thereof | |
| CN104198716A (en) | Animal brucella antibody gold mark rapid detection kit | |
| CN202916285U (en) | Whole blood immunochromatography device | |
| CN110133279A (en) | A kind of joint inspection colloidal gold strip detecting transgenosis BT albumen and CP4-EPSPS albumen | |
| CN104977407A (en) | Colloidal gold immunochromatography test paper strip for detecting tetracycline drugs, and preparation method thereof | |
| CN203148949U (en) | Rapid detection test paper card for dexamethasone immune chromatography | |
| CN102323262A (en) | Phenolethanolamine A rapid detection chip, and detection method thereof | |
| CN108226468A (en) | A kind of test strips for detecting NT-proBNP and preparation method and application | |
| CN107271665A (en) | A kind of test strips for detecting salbutamol and its application | |
| CN102213723A (en) | Doxycycline detection kit and preparation method thereof | |
| CN103412124A (en) | Aflatoxin M1 gold label quick detectiontest card and preparation method and application thereof | |
| CN105334323A (en) | Method and test strip for detecting zilpaterol, and application of test strip | |
| CN101706499A (en) | FLAG fusion tag colloidal gold test strip and preparation method thereof | |
| CN203148955U (en) | Test strip for quickly testing clonidine through immunochromatography | |
| CN204314301U (en) | The immuno-chromatographic test paper strip of a kind of synchronous detection vomitoxin and fumonisin | |
| CN106526166A (en) | Rapid detection of lean meat powder in pork | |
| CN204314300U (en) | The immuno-chromatographic test paper strip of a kind of synchronous detection ochratoxin A and zearalenone | |
| CN104090100B (en) | Brucella antibody gold labeled quick detection reagent box | |
| CN203148921U (en) | Rapid detection test paper card for benzopyrene immune chromatography | |
| CN204044161U (en) | Deoxynivalenol enol colloidal gold reagent plate in a kind of grain and oil of detection fast | |
| CN110927379A (en) | Duplex test strip for detecting morphine and ketamine as well as preparation method and application method thereof | |
| CN102507940B (en) | Method for quickly detecting chloramphenicol in cosmetics | |
| CN202794181U (en) | Test paper card for rapid detection of aflatoxin B1 residue |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130821 Termination date: 20150228 |
|
| EXPY | Termination of patent right or utility model |