CN106771154A - The detection card and its detection method of AVM in a kind of detection tableware - Google Patents
The detection card and its detection method of AVM in a kind of detection tableware Download PDFInfo
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- CN106771154A CN106771154A CN201611072105.3A CN201611072105A CN106771154A CN 106771154 A CN106771154 A CN 106771154A CN 201611072105 A CN201611072105 A CN 201611072105A CN 106771154 A CN106771154 A CN 106771154A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/36—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Actinomyces; from Streptomyces (G)
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Abstract
The invention discloses a kind of detection card for detecting AVM in tableware, including base plate, adsorptive pads, object-putting layer, diversion layer and cellulose nitrate layer, the base plate is positioned over bottom, Far Left on base plate is provided with adsorptive pads, object-putting layer is provided with the right of adsorptive pads, diversion layer is provided with the right of object-putting layer, the cellulose nitrate layer is provided with the low order end on base plate.The present invention can not only quickly detect the residual information of AVM in tableware, and with incomparable simple of chromatographic detection method, quickly, the advantages of being easy to examination a large amount of samples, it is one kind simple in animals and plants food or tableware, the new technique of quick detection AVM medicament residue, suitable for correlation quarantine detection department, import and export the quick detection of AVM medicament residue in quarantine detection department and laboratory or extensive examination, simply, quickly, accurately, rate of accuracy reached more than 99%, show that present invention detection card has very strong actual application value, economic and social benefit is huge.
Description
Technical field
The present invention relates to one kind detection card, the detection card of AVM and its detection side in specifically a kind of detection tableware
Method.
Background technology
AVM is produced as a kind of ten hexa-atomic macrolide novel pesticides by Avid kyowamycin fermentation in streptomycete
It is raw, because chemical constitution is novel, mechanism of action is unique（The nervous physiology activity of polypide is disturbed, stimulates release GABA, from
And suppress the nerve conduction of arthropod）, insecticidal activity it is strong, with wide spectrum, efficiently, holding effect, it is comparatively safe the features such as, meet existing
For ecological agricultural chemical requirement, it is widely used in the various nematodes in inside and outside, arthropod class parasite and insect etc. are killed.It is complete at present
The positive large area of ball promotes the use of AVM product.But as pest resistance is improved, AVM dosage is continuously increased, to ring
Border and the mankind, animals and plants harm also increasingly manifest, and are mainly shown as medicament residue, humans and animals maincenter and the periphery of field soil
Nervous symptoms, such as tremble, incoordination, mental depression, height stupor or even dead, and produce embryotoxicity.By world health
Tissue（WTO）5 grades of criteria for classifications, are classified as cytotoxic compound high.
In consideration of it, the detection of AVM medicament residue turns into state key monitor control index, AVM hereinafter in food and tableware product
Rhzomorph method for detecting residue also turns into primary study content.At present, it is mainly chromatogram inspection on AVM method for detecting residue
Survey method and immunodetection, chromatographic detection have thin-layered chromatography, liquid chromatography, Liquid Chromatography-Mass Spectrometry etc., these
Be present the deficiencies such as sample pre-treatments are complicated, need professional operator, need expensive instrument cost higher in detection method, be usually used in
The confirmatory analysis of AVM medicament residue；In the prior art, substantial amounts of grinding has been made for the detection of AVM
Study carefully, include the detection kit of AVM, the preparation method of kit and detection AVM is carried out using kit
Method.But, in the prior art, the detection method of the AVM for generally using has：Thin-layered chromatography (TLC), gas phase
Chromatography (GC), high performance liquid chromatography (HPLC), gas-matter is online (GC/MS), and liquid-matter is online (HPLC/MS), capillary electricity
Swimming (CE) etc..Detection in aforementioned manners has complicated instrument and equipment, Sample pretreatment and determines the loaded down with trivial details defect of operation, uncomfortable
On-site supervision and great amount of samples examination, and high cost are closed, it is promoted the use of and is restricted.
AVM detection card is that modern monoclonal antibody technique, new is merged in colloidal gold immunochromatographimethod technical foundation
A kind of simple, quick, economic immunologic detection method that material technology is set up, the test strip is operated compared with ELISA more
Simply, quickly, be more easy to judged result, be technical method of another new, economic, environmentally friendly detection AVM.Current Ah
Dimension rhzomorph colloidal gold colloidal gold detection test paper strip is still at home and abroad blank.
The content of the invention
It is an object of the invention to provide a kind of detection card and its detection method for detecting AVM in tableware, to solve
The problem proposed in above-mentioned background technology.
To achieve the above object, the present invention provides following technical scheme：
The detection card of AVM in a kind of detection tableware, including base plate, adsorptive pads, object-putting layer, diversion layer and cellulose nitrate layer,
The base plate is positioned over bottom, and the Far Left on base plate is provided with adsorptive pads, and object-putting layer is provided with the right of adsorptive pads, object-putting layer
The right is provided with diversion layer, and the cellulose nitrate layer is provided with the midpoint position of the low order end on base plate, diversion layer and cellulose nitrate layer
Put and be coated with right part overlay film, the point midway of adsorptive pads, object-putting layer and cellulose nitrate layer is coated with left part overlay film, cellulose nitrate layer
On be coated with the detection of a nature controlling line for anti-rat immune globulin antibody, AVM protein conjugate from top to bottom
Line, absorption is coated with colloid gold label thing on object-putting layer.
As further scheme of the invention：The material of the base plate is PVC board, and the material of the adsorptive pads is diversion glass
Glass fiber, the material of the object-putting layer is carrier glass fiber, and the material of the diversion layer is water suction cotton starch plate.
As further scheme of the invention：Detection method is：Tableware to be detected is immersed in distilled water solution first
Middle 60min, water sample takes 5.0mL filtering water samples and is placed in conical centrifuge tubes of the 15mL with plug through 0.22 μm of membrane filtration, will contain
The 1.0mL acetone for having 70.0 μ L chloroforms is rapidly injected in centrifuge tube by microsyringe, and be then vortexed 10s, mixing
Liquid is mutually extracted in chloroformic solution from water, stands 10min, and the extractant chloroform being dispersed in water phase deposits to test tube bottom
Portion, with microsyringe draw extractant to 10mL centrifuge tubes in, then to filtering water sample in add contain 70 μ L chloroforms
1mL acetone, vortex 10s stands 10min, removes a layer extractant, merges extraction solution, nitrogen drying, then with 50 μ L methyl alcohol
Dissolving, vortex 30s, as solution to be detected, will detect that the adsorptive pads of card are immersed in 5min in above-mentioned solution, take out observation.
As further scheme of the invention：Described macrolide antibiotic detection line and described beta-lactam
Antibiotic detection line Ofloxacin class antibiotic detection line side by side, described is with described carbostyril antibiotic detection line simultaneously
Row, described sulfa antibiotics detection line are with described cephalosporin analog antibiotic detection line side by side.
As further scheme of the invention：Preparation method is that A prepares monoclonal antibody colloid gold label thing：Will be with
The 2 of stereometer：1 part of mouse ascites and 2 parts of 60mM acetate buffers containing anti-AVM protein conjugate monoclonal antibody
Liquid mixes, and is stirred at room temperature down and is added dropwise over octanoic acid, and 33 μ l octanoic acids are added per 1ml mouse ascites, mixes, and room temperature is placed 30 minutes,
15000 revs/min 4 DEG C are centrifuged 20 minutes, obtain supernatant, and supernatant is filtered with mineral wool, abandons precipitation, and the supernatant after filtering is used
NaOH adjusts pH value to 7.2, adds ammonium sulfate, and 0.277g ammonium sulfate is added per 1mlpH7.2 supernatants, quickly dissolves ammonium sulfate,
It is stirred at room temperature 30 minutes, 15000 revs/min 4 DEG C are centrifuged 20 minutes, abandon supernatant, obtains sediment, sediment original mouse ascites 1/
The 0.01M phosphate buffers dissolving of 2 volumes, then with the dialysis 48 hours of 4 DEG C of 0.01M phosphate buffers, into monoclonal
Antibody colloidal gold label；B prepares colloidal gold solution：Distilled water is boiled 4~6 minutes, matter is added in every 1000mL distilled water
The chlorauric acid solution 10ml of the concentration 1% and 15~20ml of citric acid three sodium solution of mass concentration 1% is measured, stirring to solution is in dark red
Color, is cooled to room temperature, pH value to the isoelectric point or alkalescent of anti-AVM protein conjugate monoclonal antibody is adjusted, into anti-AVM hereinafter
The colloidal gold solution of rhzomorph protein conjugate monoclonal antibody preliminary making；C prepares colloid gold label thing：Take step B preparations
Colloidal gold solution 100ml, the μ g/ml of monoclonal antibody colloid gold label thing 1~10 for adding step A to prepare, 2 points of stirring reaction
Clock, adds the bovine serum albumin(BSA) of mass concentration 10%, and the cow's serum of 15 μ l mass concentrations 10% is added in every 1ml colloidal gold solutions
Albumin, 15000 revs/min are centrifuged 40 minutes at 4 DEG C, abandon supernatant, must precipitate, and precipitation is diluted to former colloidal gold solution body with PBS
Long-pending 30%~40%, into collaurum label, D, solidification collaurum label：Coating colloid is adsorbed on carrier glass fibrage
Golden label, is dried, and colloid gold label thing is solidificated on carrier glass fibrage, E, coating film preparation：Take the immune ball of anti-mouse
Protein antibodies, AVM protein conjugate, are coated on cellulose nitrate layer respectively, coated one is formed from top to bottom and is resisted
The detection line of the nature controlling line of rat immune globulin antibody, AVM protein conjugate, wherein, anti-rat immune globulin
Antibody coating concentration is 0.5~5mg/ml, and AVM protein conjugate coating concentration is 0.05~2mg/ml；F, cutting：
To include being adsorbed with the carrier glass fibrage of colloid gold label thing, be coated with anti-rat immune globulin antibody, AVM albumen
Matter conjugate cellulose nitrate layer after test strips carrier cut into strip, test strips of the present invention.
Compared with prior art, the beneficial effects of the invention are as follows：The present invention can not only quickly detect in tableware Ah
Dimension the residual information of rhzomorph, and have the advantages that chromatographic detection method it is incomparable it is simple, quick, be easy to a large amount of samples of examination,
It is a kind of simple, new technique of quick detection AVM medicament residue in animals and plants food or tableware, it is adaptable to related
Quarantine detection department, the quick detection for importing and exporting AVM medicament residue in quarantine detection department and laboratory or extensive sieve
Look into, simple, quick, accurate, rate of accuracy reached more than 99%, show that present invention detection card has very strong actual application value, it is economical
It is huge with social benefit.
Brief description of the drawings
Fig. 1 is overall structure figure of the invention.
Specific embodiment
The technical scheme of this patent is described in more detail with reference to specific embodiment.
Fig. 1 is referred to, the detection card of AVM in a kind of detection tableware, including base plate, adsorptive pads, object-putting layer, diversion
Layer and cellulose nitrate layer, the base plate are positioned over bottom, and the Far Left on base plate is provided with adsorptive pads, is provided with the right of adsorptive pads
Object-putting layer, is provided with diversion layer on the right of object-putting layer, the cellulose nitrate layer is provided with the low order end on base plate, diversion layer and nitric acid
The point midway of fibrage is coated with right part overlay film, and the point midway of adsorptive pads, object-putting layer and cellulose nitrate layer is coated with left part
Overlay film, a nature controlling line for anti-rat immune globulin antibody, an AVM egg are coated with cellulose nitrate layer from top to bottom
The detection line of white matter conjugate, absorption is coated with colloid gold label thing on object-putting layer.
The material of base plate is PVC board, and the material of the adsorptive pads is diversion glass fibre, and the material of the object-putting layer is load
Body glass fibre, the material of the diversion layer is water suction cotton starch plate.
Detection method is：Tableware to be detected is immersed in 60min in distilled water solution first, water sample is through 0.22 μm of filter
Membrane filtration, takes 5.0mL filtering water samples and is placed in conical centrifuge tubes of the 15mL with plug, by the 1.0mL containing 70.0 μ L chloroforms
Acetone is rapidly injected in centrifuge tube by microsyringe, and be then vortexed 10s, and mixed liquor is mutually extracted to chloroformic solution from water
In, 10min is stood, the extractant chloroform being dispersed in water phase deposits to test tube bottom, and it is molten to draw extraction with microsyringe
In agent to 10mL centrifuge tubes, then to the 1mL acetone containing 70 μ L chloroforms is added in filtering water sample, vortex 10s stands
10min, removes a layer extractant, merges extraction solution, nitrogen drying, then dissolved with 50 μ L methyl alcohol, vortex 30s, as to be checked
Solution is surveyed, will detect that the adsorptive pads of card are immersed in 5min in above-mentioned solution, take out observation.
Ofloxacin of the macrolide antibiotic detection line with described beta-Lactam antibiotic detection line side by side, described
Class antibiotic detection line and described carbostyril antibiotic detection line sulfa antibiotics detection line side by side, described with it is described
Cephalosporin analog antibiotic detection line side by side.
Preparation method is that A prepares monoclonal antibody colloid gold label thing：By 2 by volume：1 part contains anti-Avermectin
The mouse ascites of cellulose protein conjugate monoclonal antibody mix with 2 parts of 60mM acetate buffer solutions, are stirred at room temperature down and are added dropwise over
Octanoic acid, 33 μ l octanoic acids are added per 1ml mouse ascites, are mixed, and room temperature is placed 30 minutes, and 15000 revs/min 4 DEG C are centrifuged 20 minutes, obtain
Supernatant, supernatant is filtered with mineral wool, abandons precipitation, and the supernatant after filtering adjusts pH value to 7.2 with NaOH, adds ammonium sulfate,
0.277g ammonium sulfate is added per 1mlpH7.2 supernatants, quickly dissolves ammonium sulfate, be stirred at room temperature 30 minutes, 15000 rev/min 4
DEG C centrifugation 20 minutes, abandons supernatant, obtains sediment, and sediment is molten with the 0.01M phosphate buffers of the former volume of mouse ascites 1/2
Liquid dissolves, then with the dialysis 48 hours of 4 DEG C of 0.01M phosphate buffers, into monoclonal antibody colloid gold label thing；B prepares colloid
Gold solution：Distilled water is boiled 4~6 minutes, per 1000mL distilled water in add mass concentration 1% chlorauric acid solution 10ml and
15~the 20ml of citric acid three sodium solution of mass concentration 1%, stirring to solution is in peony, is cooled to room temperature, adjusts pH value to anti-AVM hereinafter
The isoelectric point or alkalescent of rhzomorph protein conjugate monoclonal antibody, into anti-AVM protein conjugate monoclonal antibody
The colloidal gold solution of preliminary making；C prepares colloid gold label thing：The colloidal gold solution 100ml of step B preparations is taken, step A systems are added
The standby μ g/ml of monoclonal antibody colloid gold label thing 1~10, stirring reaction 2 minutes adds the ox blood of mass concentration 10% pure
Albumen, adds the bovine serum albumin(BSA) of 15 μ l mass concentrations 10% in every 1ml colloidal gold solutions, 15000 revs/min of centrifugations 40 at 4 DEG C
Minute, supernatant being abandoned, must precipitate, precipitation is diluted to the 30%~40% of former colloidal gold solution volume with PBS, into collaurum label,
D, solidification collaurum label：Coating colloid gold label thing is adsorbed on carrier glass fibrage, is dried, by colloid gold label thing
It is solidificated on carrier glass fibrage, E, coating film preparation：Take anti-rat immune globulin antibody, AVM protein molecule
Thing, is coated on cellulose nitrate layer respectively, and coated one nature controlling line, one of anti-rat immune globulin antibody are formed from top to bottom
The detection line of bar AVM protein conjugate, wherein, anti-rat immune globulin antibody coating concentration is 0.5~5mg/ml,
AVM protein conjugate coating concentration is 0.05~2mg/ml；F, cutting：To include being adsorbed with colloid gold label thing
Carrier glass fibrage, the examination being coated with after the cellulose nitrate layer of anti-rat immune globulin antibody, AVM protein conjugate
Paper slip carrier cuts into strip, test strips of the present invention.
Operation principle of the invention is：The present invention can not only quickly detect the residual information of AVM in tableware, and
And have the advantages that chromatographic detection method it is incomparable it is simple, quick, be easy to a large amount of samples of examination, be a kind of in animals and plants food
The simple, new technique of quick detection AVM medicament residue in product or soil, it is adaptable to correlation quarantine detection department, turnover
The quick detection of AVM medicament residue or extensive examination in mouth quarantine detection department and laboratory, it is simple, quick, accurate
Really, detected through to field soil at 58 batches of insecticidal materials and 38, rate of accuracy reached more than 99%, show test strips tool of the present invention
There is very strong actual application value, economic and social benefit is huge.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be in other specific forms realized.Therefore, no matter
From the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is by appended power
Profit requires to be limited rather than described above, it is intended that all in the implication and scope of the equivalency of claim by falling
Change is included in the present invention.Any reference in claim should not be considered as the claim involved by limitation.
Moreover, it will be appreciated that although the present specification is described in terms of embodiments, not each implementation method is only wrapped
Containing an independent technical scheme, this narrating mode of specification is only that for clarity, those skilled in the art should
Specification an as entirety, the technical scheme in each embodiment can also be formed into those skilled in the art through appropriately combined
May be appreciated other embodiment.
Claims (4)
1. a kind of detection card for detecting AVM in tableware, including base plate, adsorptive pads, object-putting layer, diversion layer and cellulose nitrate
Layer, it is characterised in that the base plate is positioned over bottom, and the Far Left on base plate is provided with adsorptive pads, is provided with the right of adsorptive pads
Object-putting layer, is provided with diversion layer on the right of object-putting layer, the cellulose nitrate layer is provided with the low order end on base plate, diversion layer and nitric acid
The point midway of fibrage is coated with right part overlay film, and the point midway of adsorptive pads, object-putting layer and cellulose nitrate layer is coated with left part
Overlay film, a nature controlling line for anti-rat immune globulin antibody, an AVM egg are coated with cellulose nitrate layer from top to bottom
The detection line of white matter conjugate, absorption is coated with colloid gold label thing on object-putting layer.
2. it is according to claim 1 it is a kind of detect tableware in AVM detection card, it is characterised in that the base plate
Material is PVC board, and the material of the adsorptive pads is diversion glass fibre, and the material of the object-putting layer is carrier glass fiber, institute
It is water suction cotton starch plate to state the material of diversion layer.
3. it is according to claim 1 it is a kind of detect tableware in AVM detection card, it is characterised in that detection method
It is：Tableware to be detected is immersed in 60min in distilled water solution first, water sample takes 5.0mL mistakes through 0.22 μm of membrane filtration
Drainage sample is placed in conical centrifuge tubes of the 15mL with plug, and the 1.0mL acetone containing 70.0 μ L chloroforms is passed through into micro-sampling
Device is rapidly injected in centrifuge tube, and be then vortexed 10s, and mixed liquor is mutually extracted in chloroformic solution from water, stands 10min, point
The extractant chloroform being dispersed in water phase deposits to test tube bottom, and extractant to 10mL centrifuge tubes is drawn with microsyringe
In, then to adding the 1mL acetone containing 70 μ L chloroforms, vortex 10s to stand 10min in filtering water sample, remove layer extraction molten
Agent, merges extraction solution, nitrogen drying, then is dissolved with 50 μ L methyl alcohol, vortex 30s, as solution to be detected, will detect the suction of card
Water cushion is immersed in 5min in above-mentioned solution, takes out observation.
4. it is according to claim 1 it is a kind of detect tableware in AVM detection card, it is characterised in that preparation method
It is that A prepares monoclonal antibody colloid gold label thing：By 2 by volume：1 part contains anti-AVM protein conjugate list
The mouse ascites of clonal antibody mix with 2 parts of 60mM acetate buffer solutions, are stirred at room temperature down and are added dropwise over octanoic acid, per 1ml mouse abdomens
Water adds 33 μ l octanoic acids, mixes, and room temperature is placed 30 minutes, and 15000 revs/min 4 DEG C are centrifuged 20 minutes, obtains supernatant, and supernatant is used
Mineral wool is filtered, and abandons precipitation, and the supernatant after filtering adjusts pH value to 7.2 with NaOH, adds ammonium sulfate, per 1mlpH7.2 supernatants
0.277g ammonium sulfate is added, quickly dissolves ammonium sulfate, be stirred at room temperature 30 minutes, 15000 revs/min 4 DEG C are centrifuged 20 minutes, abandon
Clear liquid, obtains sediment, and the sediment 0.01M phosphate buffers of the former volume of mouse ascites 1/2 dissolve, then use 0.01M
4 DEG C of phosphate buffer is dialysed 48 hours, into monoclonal antibody colloid gold label thing；B prepares colloidal gold solution：Distilled water is boiled
Boiling 4~6 minutes, adds the chlorauric acid solution 10ml of mass concentration 1% and the citric acid of mass concentration 1% in every 1000mL distilled water
Three 15~20ml of sodium solution, stirring to solution is in peony, is cooled to room temperature, adjusts pH value to anti-AVM protein conjugate list
The isoelectric point or alkalescent of clonal antibody, the collaurum into anti-AVM protein conjugate monoclonal antibody preliminary making are molten
Liquid；C prepares colloid gold label thing：The colloidal gold solution 100ml of step B preparations is taken, the monoclonal antibody glue for adding step A to prepare
The body gold μ g/ml of label 1~10, stirring reaction 2 minutes adds the bovine serum albumin(BSA) of mass concentration 10%, per 1ml collaurums
The bovine serum albumin(BSA) of 15 μ l mass concentrations 10% is added in solution, 15000 revs/min are centrifuged 40 minutes at 4 DEG C, abandon supernatant, obtain heavy
Form sediment, precipitation is diluted to the 30%~40% of former colloidal gold solution volume with PBS, into collaurum label, D, solidification colloid gold label
Thing：Coating colloid gold label thing is adsorbed on carrier glass fibrage, is dried, it is fine that colloid gold label thing is solidificated in into carrier glass
On dimension layer, E, coating film preparation：Anti- rat immune globulin antibody, AVM protein conjugate are taken, nitric acid is coated in respectively
On fibrage, coated one nature controlling line of anti-rat immune globulin antibody, an AVM protein are formed from top to bottom
The detection line of conjugate, wherein, anti-rat immune globulin antibody coating concentration is 0.5~5mg/ml, AVM protein idol
Connection thing coating concentration is 0.05~2mg/ml；F, cutting：Carrier glass fibrage, the bag of colloid gold label thing will be included being adsorbed with
By the test strips carrier cut growth after anti-rat immune globulin antibody, the cellulose nitrate layer of AVM protein conjugate
Bar, test strips of the present invention.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201611072105.3A CN106771154A (en) | 2016-11-29 | 2016-11-29 | The detection card and its detection method of AVM in a kind of detection tableware |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201611072105.3A CN106771154A (en) | 2016-11-29 | 2016-11-29 | The detection card and its detection method of AVM in a kind of detection tableware |
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| EP0450936A1 (en) * | 1990-04-05 | 1991-10-09 | Merck & Co. Inc. | Monoclonal antibody to avermectins |
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| CN202720231U (en) * | 2012-06-28 | 2013-02-06 | 艾博生物医药(杭州)有限公司 | Test strip for testing specimen |
| CN103513030A (en) * | 2012-06-28 | 2014-01-15 | 艾博生物医药(杭州)有限公司 | Test strip for detecting samples |
| CN104407146A (en) * | 2014-12-02 | 2015-03-11 | 河南省科学院生物研究所有限责任公司 | Preparation method of abamectin colloidal gold test strip |
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| CN106046083A (en) * | 2015-06-10 | 2016-10-26 | 北京维德维康生物技术有限公司 | Avermectin artificial antigen and preparation method and application thereof |
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2016
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|---|---|---|---|---|
| EP0450936A1 (en) * | 1990-04-05 | 1991-10-09 | Merck & Co. Inc. | Monoclonal antibody to avermectins |
| CN101448927A (en) * | 2006-05-18 | 2009-06-03 | 雷克特本克斯尔荷兰有限公司 | Detergent product and process for its preparation and use thereof |
| CN202720231U (en) * | 2012-06-28 | 2013-02-06 | 艾博生物医药(杭州)有限公司 | Test strip for testing specimen |
| CN103513030A (en) * | 2012-06-28 | 2014-01-15 | 艾博生物医药(杭州)有限公司 | Test strip for detecting samples |
| CN104407146A (en) * | 2014-12-02 | 2015-03-11 | 河南省科学院生物研究所有限责任公司 | Preparation method of abamectin colloidal gold test strip |
| CN106046083A (en) * | 2015-06-10 | 2016-10-26 | 北京维德维康生物技术有限公司 | Avermectin artificial antigen and preparation method and application thereof |
| CN105911184A (en) * | 2016-04-20 | 2016-08-31 | 天津农学院 | Method of detecting residue of abamectin in water |
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Application publication date: 20170531 |