CN115524486A - Immunity chromatography test paper - Google Patents
Immunity chromatography test paper Download PDFInfo
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- CN115524486A CN115524486A CN202211416542.8A CN202211416542A CN115524486A CN 115524486 A CN115524486 A CN 115524486A CN 202211416542 A CN202211416542 A CN 202211416542A CN 115524486 A CN115524486 A CN 115524486A
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- pad
- detection
- membrane
- immunochromatographic test
- quality control
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- Immunology (AREA)
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- Engineering & Computer Science (AREA)
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- Urology & Nephrology (AREA)
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- General Health & Medical Sciences (AREA)
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- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
Abstract
The invention discloses an immunochromatographic test paper. The immunochromatographic test paper comprises a sample pad; an entrapment pad attached to the sample pad; the detection membrane is connected to the interception pad, and a detection line and a quality control line are sequentially arranged on the detection membrane; a bibulous pad attached to the detection membrane. The immunochromatographic test paper provided by the invention can improve the sensitivity and reduce the specificity, so that the accuracy is improved, and finally the product performance is improved.
Description
Technical Field
The invention relates to the technical field of medical detection, in particular to immunochromatography test paper.
Background
Immunochromatography is divided into longitudinal chromatography and lateral chromatography. The immune lateral chromatography diagnostic technique is suitable for various point-of-care tests (POCT) or field use as a stable and practical technique, and is mainly divided into a colloidal gold immune lateral chromatography method, a common fluorescence immune lateral chromatography method, a time-resolved fluorescence immune lateral chromatography method, an up-conversion luminescence immune lateral chromatography method, a quantum dot fluorescence immune lateral chromatography method and the like according to a marked part, and a nitrocellulose membrane, a composite material (fusion 5), a micro-fluidic method and the like according to a coated part.
However, quantitative detection has higher and higher requirements on project precision and repeatability, and through the development of many years, various fluorescence immune lateral chromatography methods are developed. Has become the mainstream advanced technology and is advocated by many companies. However, in the fluorescent (colloidal gold) marker in the prior art, a certain proportion of 'micro aggregated markers' exist, and particularly, when chromatography is performed from bottom to top, in all-in-one items, the markers are more, the baseline is higher, the area obtained by T-line integral is interfered, and then the interference on specificity, sensitivity and accuracy is caused, and in severe cases, aggregated fluorescence can even block the nitrocellulose membrane, and normal fluorescence chromatography is influenced.
Disclosure of Invention
In order to solve the technical problem that the fluorescence (colloidal gold) marker in the prior art interferes with specificity, sensitivity and accuracy, the invention provides the immunochromatographic test paper which can reduce the baseline of an immunochromatographic product, particularly an all-in-one item, reduce the interference on the area calculation by the integral of a T line and further improve the specificity, sensitivity and accuracy of detection.
The invention provides an immunochromatographic test paper, which comprises a sample pad; an entrapment pad attached to the sample pad; the detection membrane is connected to the interception pad, and a detection line and a quality control line are sequentially arranged on the detection membrane; a bibulous pad attached to the detection membrane.
In a preferred embodiment of the immunochromatographic test strip provided by the present invention, the detection membrane is a nitrocellulose membrane, the detection membrane is coated with a detection antibody and a quality control antibody, and the membrane-dividing concentration ratio between the detection membrane and the quality control antibody is (0.5-3): (0.5-3).
In a preferred embodiment of the immunochromatographic test strip provided by the present invention, the retention pad is closer to the sample pad than the detection membrane; the quality control line is closer to the absorbent pad than the detection line.
In a preferred embodiment of the immunochromatographic test strip provided by the present invention, the distance between the trapping pad and the detection line is 3-10mm, and the distance between the quality control line and the detection line is 3-6mm.
In a preferred embodiment of the immunochromatographic test strip provided by the present invention, the sample pad is a glass cellulose membrane or a polyester membrane; the water absorption pad is absorbent paper; the kit also comprises a marker pad and a bottom plate, wherein the marker pad is fixedly connected between the sample pad and the interception pad; the detection membrane, the sample pad, the marker pad, the interception pad and the absorbent pad are all fixed on the bottom plate.
Compared with the prior art, the immunochromatographic test paper provided by the invention has the following beneficial effects: by adding an interception pad (filter pad) between the marker pad and the detection membrane, the filter pad can intercept most of 'micro-aggregation markers', thereby greatly reducing the baseline and the background interference; therefore, the sensitivity is improved, the specificity is reduced, the accuracy is improved, and finally the product performance is improved.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments are briefly introduced below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without inventive efforts, wherein:
FIG. 1 is a schematic structural diagram of the immunochromatographic test strip provided by the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1, fig. 1 is a schematic structural diagram of an immunochromatographic test strip provided in the present invention. The immunochromatographic test paper comprises a glass cellulose membrane 2, and the size of the immunochromatographic test paper is set as follows: the length is 10-25mm, the width is 4-6mm, and the thickness is 500-1000 μm;
the marker pad 6 is connected to the glass cellulose membrane 2, the material of the marker pad 6 is glass fiber, and the size of the marker pad is set as follows: the length is 5-15mm, the width is 4-6mm, and the thickness is 500-1000 μm;
the interception pad 5 is connected with the marker pad 6, and the material of the interception pad 5 is a blood filtration pad; the dimensions are set as follows: the length is 5-15mm, the width is 4-6mm, and the thickness is 500-1000 μm;
a nitrocellulose membrane 3 attached to the glass cellulose membrane 2 and sized as follows: the length is 20-35mm, the width is 4-6mm, and the thickness is 0.2-0.3mm; the nitrocellulose membrane 3 is sequentially provided with a detection line 31 and a quality control line 32, the distance between the interception pad 5 and the detection line 31 is 3-10mm, and the quality control line 32 is close to the absorbent paper 4 and is 3-6mm away from the detection line;
a water absorbent paper 4 attached to the nitrocellulose membrane 3 and having the following dimensions: the length is 10-30mm, the width is 4-6mm, and the thickness is 900-1800 μm; the water absorption paper 4 is made of glass fiber and cotton linter.
The cellulose nitrate membrane 3 is respectively coated with a detection antibody and a quality control antibody which are respectively used as a detection line 31 and a quality control line 32, and the membrane scratching concentration ratio between the detection antibody and the quality control antibody is (0.5-3): (0.5-3). The quality control antibody is an anti-mouse IgG polyclonal antibody, goat anti-chicken IgY, avidin or streptavidin.
The retention pad is closer to the sample pad than the detection membrane; the retention pad is closer to the absorbent pad than the marker pad.
The immunochromatographic test paper further comprises a bottom plate 1, and the size of the bottom plate is as follows: the length is 20-30cm, the width is 6-8cm, and the thickness is 0.25-0.5mm; the nitrocellulose membrane 3, the glass cellulose membrane 2, the marker pad 6, the retention pad 5 and the absorbent paper 4 are all fixed on the bottom plate 1.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by using the contents of the present specification and the accompanying drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (5)
1. An immunochromatographic test paper characterized by comprising a sample pad; an entrapment pad attached to the sample pad; the detection membrane is connected to the interception pad, and a detection line and a quality control line are sequentially arranged on the detection membrane; a bibulous pad attached to the detection membrane.
2. The immunochromatographic test strip according to claim 1, wherein the detection membrane is a nitrocellulose membrane, the detection membrane is coated with a detection antibody and a quality control antibody, and the ratio of the scratch concentration between the detection antibody and the quality control antibody is (0.5-3): (0.5-3).
3. The immunochromatographic test strip of claim 1, wherein the retention pad is closer to the sample pad than the detection membrane; the quality control line is closer to the absorbent pad than the detection line.
4. The immunochromatographic test strip of claim 1, wherein the distance between the trapping pad and the detection line is 3-10mm, and the distance between the quality control line and the detection line is 3-6mm.
5. The immunochromatographic test strip according to claim 1, wherein the sample pad is a glass cellulose membrane or a polyester membrane; the water absorption pad is absorbent paper; the kit also comprises a marker pad and a bottom plate, wherein the marker pad is fixedly connected between the sample pad and the interception pad; the detection membrane, the sample pad, the marker pad, the interception pad and the absorbent pad are all fixed on the bottom plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211416542.8A CN115524486A (en) | 2022-11-13 | 2022-11-13 | Immunity chromatography test paper |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211416542.8A CN115524486A (en) | 2022-11-13 | 2022-11-13 | Immunity chromatography test paper |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115524486A true CN115524486A (en) | 2022-12-27 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211416542.8A Withdrawn CN115524486A (en) | 2022-11-13 | 2022-11-13 | Immunity chromatography test paper |
Country Status (1)
| Country | Link |
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| CN (1) | CN115524486A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117269511A (en) * | 2023-11-21 | 2023-12-22 | 江苏硕世生物科技股份有限公司 | Beta 2-microglobulin fluorescent quantitative detection kit |
-
2022
- 2022-11-13 CN CN202211416542.8A patent/CN115524486A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117269511A (en) * | 2023-11-21 | 2023-12-22 | 江苏硕世生物科技股份有限公司 | Beta 2-microglobulin fluorescent quantitative detection kit |
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Legal Events
| Date | Code | Title | Description |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20221227 |
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| WW01 | Invention patent application withdrawn after publication |