CN212228955U - Simple detection device - Google Patents
Simple detection device Download PDFInfo
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- CN212228955U CN212228955U CN202020147347.XU CN202020147347U CN212228955U CN 212228955 U CN212228955 U CN 212228955U CN 202020147347 U CN202020147347 U CN 202020147347U CN 212228955 U CN212228955 U CN 212228955U
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Abstract
The utility model discloses a simple detection device, which comprises a lower cover, a test strip and an upper cover; the test strip is arranged on the lower cover and comprises a bottom plate, a sample pad, a release pad, an NC membrane and absorbent paper, wherein the bottom plate is arranged on the lower cover, and the sample pad, the release pad, the NC membrane and the absorbent paper are all arranged on the bottom plate and are sequentially connected; the upper cover is arranged on the lower cover and covers the test strip, and a reagent pad is arranged on the upper cover and is opposite to the NC membrane in a matching way. Through utilizing sample pad, release pad, NC membrane and absorbent paper all to set up on the bottom plate and connect gradually and form the examination strip to the cooperation is covered and is provided with the reagent pad, makes when examining, does not need fluorescence microballon mark antibody, with the enzyme-labeled can, detect simple and conveniently.
Description
Technical Field
The utility model belongs to the technical field of the detection device technique and specifically relates to indicate a simple and easy type detection device.
Background
The fluorescent quantitative chromatographic test strip technology is an improvement on colloidal gold chromatographic test strip technology, and overcomes the problems of low sensitivity, large background interference, large batch-to-batch and batch-to-batch difference and the like of colloidal gold test strips. The fluorescence quantitative chromatography test strip is mostly marked with antigen or antibody by using fluorescent latex particles at present. The fluorescent particles are more uniform in size than colloidal gold, the particle-labeled antibody is firmer (the colloidal gold is non-specifically adsorbed, and the fluorescent latex particles are crosslinked with the antibody by adopting covalent bonds), the batch-to-batch particle-labeled antibody amount is easy to control, the accuracy of the whole test strip is good, and the difference between batches is small.
However, microspheres tend to have a large volume (typically around 200nm in diameter), while antibodies are on the order of about 10nm in size. The reactivity of antibodies coupled to microspheres can be greatly influenced and often changes the properties of the antibodies.
If the fluorescein molecule is directly marked on the antibody, the influence on the antibody is small, but the sensitivity of the single antibody in the case is not clinically required.
Therefore, there is a need to develop a solution to the above problems.
SUMMERY OF THE UTILITY MODEL
In view of this, the present invention is directed to the deficiency of the prior art, and the main objective of the present invention is to provide a simple detection device, which does not need fluorescent microspheres to label the antibody, and can be labeled with an enzyme, and the detection is simple and convenient.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
a simple detection device comprises a lower cover, a test strip and an upper cover; the test strip is arranged on the lower cover and comprises a bottom plate, a sample pad, a release pad, an NC membrane and absorbent paper, wherein the bottom plate is arranged on the lower cover, and the sample pad, the release pad, the NC membrane and the absorbent paper are all arranged on the bottom plate and are sequentially connected; the upper cover is arranged on the lower cover and covers the test strip, and a reagent pad is arranged on the upper cover and is opposite to the NC membrane in a matching way.
Preferably, the lower edge of the upper cover is hinged with one side edge of the lower cover, and the upper cover is arranged in a manner of being capable of being turned over up and down relative to the lower cover.
Preferably, the upper cover is a transparent cover.
Preferably, one end of the absorbent paper and one end of the release pad are respectively connected in a manner of abutting against the surfaces of the two ends of the NC membrane, and one end of the sample pad is connected in a manner of abutting against the surface of the other end of the release pad.
Preferably, the upper cover and the lower cover are both strip-shaped plate pieces, and the sample pad, the release pad, the NC membrane and the absorbent paper are sequentially and transversely arranged.
Compared with the prior art, the utility model obvious advantage and beneficial effect have, particularly, can know by above-mentioned technical scheme:
through utilizing sample pad, release pad, NC membrane and absorbent paper all to set up on the bottom plate and connect gradually and form the examination strip to the cooperation is covered and is provided with the reagent pad, makes when examining, does not need fluorescence microballon mark antibody, with the enzyme-labeled can, detect simple and conveniently.
To more clearly illustrate the structural features and functions of the present invention, the present invention will be described in detail with reference to the accompanying drawings and specific embodiments:
drawings
FIG. 1 is a perspective view of a preferred embodiment of the present invention;
FIG. 2 is a perspective view of another embodiment of the present invention;
fig. 3 is a partially enlarged schematic view of a preferred embodiment of the present invention.
The attached drawings indicate the following:
10. lower cover 20, test strip
21. Bottom plate 22, sample pad
23. Release liner 24, NC Membrane
25. Absorbent paper 30, upper cover
31. Reagent pad
Detailed Description
Referring to fig. 1 to 3, the detailed structure of the preferred embodiment of the present invention is shown, which includes a lower cover 10, a test strip 20 and an upper cover 30.
The test strip 20 is arranged on the lower cover 10, the test strip 20 comprises a bottom plate 21, a sample pad 22, a release pad 23, an NC membrane 24 and absorbent paper 25, the bottom plate 21 is arranged on the lower cover 10, and the sample pad 22, the release pad 23, the NC membrane 24 and the absorbent paper 25 are all arranged on the bottom plate 21 and are sequentially connected; in this embodiment, one end of the absorbent paper 25 and one end of the release pad 23 are respectively connected against both end surfaces of the NC film 24, and one end of the sample pad 22 is connected against the other end surface of the release pad 23.
The upper cover 30 is disposed on the lower cover 10 and covers the test strip 20, and the upper cover 30 is disposed with a reagent pad 31, and the reagent pad 31 is opposite to the NC film 24. In this embodiment, the lower edge of the upper cover 30 is hinged to one side edge of the lower cover 10, the upper cover 30 is arranged to be turned over with respect to the lower cover 10, and the upper cover 30 is a transparent cover, so that it is not necessary to open the cover during detection.
And the upper cover 30 and the lower cover 10 are both strip-shaped sheets, and the sample pad 22, the release pad 23, the NC membrane 24 and the absorbent paper 25 are transversely arranged in sequence.
Detailed description the method of use of this example is as follows:
before testing, the release pad 23 is sprayed with enzyme-labeled antibody, the NC membrane 24 is coated with T line and C line according to conventional method, during testing, the sample is added to the sample pad 22, the sample redissolves the enzyme-labeled antibody on the release pad 23, then the enzyme-labeled antibody is gathered at the T line and C line on the NC membrane 24, the upper cover 30 is covered (the reagent pad 31 in the upper cover 30 is pre-coated with enzyme substrate), and after a period of time, the color development condition of the T line and the C line is observed, thus the analyte can be quantitatively or qualitatively analyzed.
The cTnI test is taken as an example to illustrate that:
a cTnI monoclonal antibody (mouse source) is marked with Hrp, purified and sprayed on glass fiber 8964, and the sprayed concentration is 1mg/ml, so that the latex pad is obtained. Another strain of cTnI monoclonal antibody and goat anti-mouse IgG was coated on millipore 135NC membrane 24 as lines T, C, respectively.
Untreated 8964 was taken as a sample pad 22, and the absorbent paper 25 was made of Shanghai gold standard SX42, and assembled into a test strip 20, which was cut to a width of 6 mm. The test strip is completed.
1mg/ml TMB, 1% Triton X100 was sprayed on the reagent pad 31 of the upper lid 30 as a substrate for Hrp. The upper cover 30, the lower cover 10, and the test strip 20 are assembled into a test device.
For testing, the cover 30 is opened, 100. mu.l of serum is dropped onto the sample pad 22, after 10min, the cover 30 is closed, incubated at 37 ℃ for 10min, and the reflectance value of line T, C is read with a reflectance meter. The concentration of cTnI in the sample can be calculated from the standard curve.
In addition to TMB, other substrates such as o-phenylenediamine may also be employed. Even the luminol and the derivative thereof are luminous substrates, so that the project has higher sensitivity.
The enzyme to be labeled may also be alkaline phosphatase, and the substrate on the reagent pad 31 may be changed to the substrate for alkaline phosphatase accordingly.
The utility model discloses a design key is: through utilizing sample pad, release pad, NC membrane and absorbent paper all to set up on the bottom plate and connect gradually and form the examination strip to the cooperation is covered and is provided with the reagent pad, makes when examining, does not need fluorescence microballon mark antibody, with the enzyme-labeled can, detect simple and conveniently.
The technical principle of the present invention is described above with reference to specific embodiments. The description is made for the purpose of illustrating the principles of the invention and should not be construed in any way as limiting the scope of the invention. Based on the explanations herein, those skilled in the art will be able to conceive of other embodiments of the present invention without any inventive effort, which would fall within the scope of the present invention.
Claims (5)
1. A simple detection device is characterized in that: comprises a lower cover, a test strip and an upper cover; the test strip is arranged on the lower cover and comprises a bottom plate, a sample pad, a release pad, an NC membrane and absorbent paper, wherein the bottom plate is arranged on the lower cover, and the sample pad, the release pad, the NC membrane and the absorbent paper are all arranged on the bottom plate and are sequentially connected; the upper cover is arranged on the lower cover and covers the test strip, and a reagent pad is arranged on the upper cover and is opposite to the NC membrane in a matching way.
2. A simplified detection device as defined in claim 1, wherein: the lower side of the upper cover is hinged with one side edge of the lower cover, and the upper cover can be arranged in a manner of being capable of being turned over up and down relative to the lower cover.
3. A simplified detection device as defined in claim 1, wherein: the upper cover is a transparent cover.
4. A simplified detection device as defined in claim 1, wherein: one end of the water absorption paper and one end of the release pad are respectively connected on the surfaces of two ends of the NC membrane in a propping mode, and one end of the sample pad is connected on the surface of the other end of the release pad in a propping mode.
5. A simplified detection device as defined in claim 1, wherein: the upper cover and the lower cover are strip-shaped plates, and the sample pad, the release pad, the NC membrane and the absorbent paper are sequentially and transversely arranged.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN202020147347.XU CN212228955U (en) | 2020-01-25 | 2020-01-25 | Simple detection device |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN202020147347.XU CN212228955U (en) | 2020-01-25 | 2020-01-25 | Simple detection device |
Publications (1)
Publication Number | Publication Date |
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CN212228955U true CN212228955U (en) | 2020-12-25 |
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CN202020147347.XU Active CN212228955U (en) | 2020-01-25 | 2020-01-25 | Simple detection device |
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CN (1) | CN212228955U (en) |
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2020
- 2020-01-25 CN CN202020147347.XU patent/CN212228955U/en active Active
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