CN205484358U - Combined two -way colloidal gold immunoassay chromatography test paper strip - Google Patents
Combined two -way colloidal gold immunoassay chromatography test paper strip Download PDFInfo
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- CN205484358U CN205484358U CN201620065094.5U CN201620065094U CN205484358U CN 205484358 U CN205484358 U CN 205484358U CN 201620065094 U CN201620065094 U CN 201620065094U CN 205484358 U CN205484358 U CN 205484358U
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- chromatography test
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Abstract
The utility model provides a combined two -way colloidal gold immunoassay chromatography test paper strip, utilizes buckle and two -way immunochromatography technique, and this test paper strip can once only detect two kinds of targets simultaneously. To two kind different main targets that the sample probably contains, can select for use the welt of different orientations and the combination of sample pad to form two -way chromatography reagent strips. The novel immunodetection equipment field that belongs to of this technique.
Description
Technical field
This utility model relates to a kind of combined two-way colloidal gold immuno-chromatography test paper strip, and the difference that may contain for sample is main
Two kinds of targets, the liner plate that can be selected for different directions forms two-dimensional chromatography reagent strip with sample pad combination.Realize detection target and detection
The controllability of equipment.
Background technology
Gold colloidal is a kind of conventional labelling technique, is that the one being applied to antigen-antibody as tracer label thing using gold colloidal is novel
Immunolabelling technique, the advantage having its uniqueness.The most it is widely used in various biological studies.Immunity at Clinical practice
Engram technology nearly all uses its labelling.Simultaneously at streaming, Electronic Speculum, immunity, molecular biology so that biochip all may
Example is used.
What the biomacromolecules such as colloid gold label substantially antibody protein were adsorbed to colloid gold particle surface is coated process.Absorption
Mechanism be probably between negative charge that gold colloid surface carried and the positive charge that protein molecule is carried and attract each other by electrostatic force,
Firm combination is i.e. formed in reaching Van der Waals force.It addition, the coarse of colloid gold particle is also advantageous that in forming the important of absorption
Condition.Due to this labeling process mainly by physisorption, thus the most obvious to the biologic activity of protein molecule
Impact.
Conventional immune colloid gold detection technique and application include: (1) immune colloid gold light microscopic staining cell suspension smear or group
Knitting section, the antibody of available colloid gold label dyes, it is possible on the basis of colloid gold label, strengthens mark with silver developer solution
Note, makes the silver atoms being reduced be deposited on marked gold grain surface, can be remarkably reinforced the sensitivity of colloid gold label.(2)
Immune colloid gold staining method for electron microscopy, the antibody of available colloid gold label or anti antibody and negative staining Virus Sample or tissue ultrathin section knot
Close, then carry out negative staining.Can be used for observation and the Viral diagnosis of morphology of virus.(3) dot immunogold filtration assay, applies micropore
Carrier made by filter membrane (such as film), first by antigen or antibody point on film, adds sample to be checked, use colloid gold label after washing after closing
The corresponding antigen of antibody test or antibody.(4) colloidal gold immunity chromatography, by solid with ribbon to specific antigen or antibody
Being scheduled on film, colloid gold label reagent (antibody or monoclonal antibody) adsorbs on pad, when sample to be checked is added to test strips
After in the sample pad of one end, moved forward by capillarity, react to each other after dissolving the colloid gold label reagent on pad,
When moving the region to fixing antigen or antibody again, the conjugate of thing to be checked and gold marked reagent occur the most therewith specific binding and
It is trapped, is gathered on detection band, colour developing result can be observed by the naked eye.Above-mentioned four kinds of methods the most progressively develop into examines
Disconnected test strips, prospect is extensive.
The assembling process of tradition gold colloidal quick measuring card is for being fixed on reagent draw-in groove lower house using polrvinyl chloride liner plate as supporting carrier
In, then sample pad, composite colloid gold pad, nitrocellulose filter and absorbent filter are arranged in order and are connected on the right side of liner plate
Surface, single gold conjugation pad, nitrocellulose filter and absorbent filter are arranged in order and are connected to the upper table of liner plate on the left of sample pad
Face obtains colloidal gold immunochromatographimethod quick measuring card.Except conventional detection labelled reagent bar, generally after clearly detecting target, then assemble
Colloid gold reagent bar, when multicomponent analysis, inefficiency, two-dimensional chromatography controls difficulty.A kind of utilization difference detection liner plate,
Realize the instant Simple assembled colloid gold reagent bar assembling, immediately coming into operation, do not have been reported that at present.
Summary of the invention
This utility model describes a kind of combined two-way colloidal gold immuno-chromatography test paper strip, the difference that may be able to contain for sample
Main two kinds of targets, select the liner plate of different directions to form two-dimensional chromatography reagent strip with sample pad combination.Described liner plate is for make in advance
For completing, immediately assemble when detection, immediately use.The detection equipment controllability for detection target can be realized.
A kind of combined two-way colloidal gold immuno-chromatography test paper strip, its agent structure comprises: liner plate and sample pad, described liner plate with
Sample pad is stand-alone assembly, and described liner plate is the stand-alone assembly assembled, and is generally stored in less than 15 DEG C Refrigerator stores.Described
Liner plate combined method but be not limited to: using polrvinyl chloride collet as support carrier be fixed in reagent draw-in groove lower house, then
Sample pad, composite colloid gold pad, nitrocellulose filter and absorbent filter are arranged in order and are connected to liner plate upper surface.Described lining
Plate is the independent two group liner plates with buckle, and often on group liner plate, the assembly pasted includes: gold conjugation pad, nitrocellulose membrane,
Absorbent filter and or other the art staff may be appreciated cotton dress analyze accessory.Described buckle be bottom liner plate outside
Protuberance position is constituted, and is shaped as annular or wedge shape.
Described sample pad includes that material is fixed in sample absorbing material and bottom, and material institute is fixed in sample absorbing material and bottom
The draw-in groove constituted.It is polyester fiber film that the raw material of material is fixed in the absorbing material of described sample pad and bottom.Described is combined double
To colloidal gold immuno-chromatography test paper strip, the bottom of gold conjugation pad is polyester fiber film, has in this polyester fiber film surface adsorption
Gold colloidal albumen, described nitrocellulose membrane pan coating has antigen or antibody protein.Described sample pad passes through draw-in groove and both direction
Liner plate buckle be connected.Envelope antigen on described nitrocellulose membrane or antibody protein are positioned at the two ends of film.Described is combined
Two-way colloidal gold immuno-chromatography test paper strip, at the two ends of described nitrocellulose membrane, is respectively present antigen described at least one or antibody
Albumen.Nitrocellulose filter is provided with the first detection line and the first control line, and celluloid is provided with the second detection line and second
Control line, coated on the first detection line is first object antibody, and coated on the second detection line is the second target antibody, first
Coated on control line is first object antibody purification, and coated on the second control line is the second target antibody purification, and antibody is suitable
Package amount is respectively 1.0-2.0 μ g, and compound gold mark probe suitable antibodies labelled amount is 10-20 μ g/mL.
Described combined two-way colloidal gold immuno-chromatography test paper strip, standby liner plate buckle with on liner plate with buckle identical, standby
Agree with sample pad draw-in groove with liner plate buckle, from arbitrarily two or more sets liner plates, select two groups of liner plates, can be with sample pad by card
Groove is connected.
Accompanying drawing explanation
In the following description to embodiment, the present invention will become apparent to, and only passes through embodiment and is described with reference to the drawings, its
In:
Fig. 1 is described combined two-way colloidal gold immuno-chromatography test paper strip side view when not combining, and wherein a is sample pad, b
For gold conjugation pad, c is a liner plate in two groups, and d is nitrocellulose membrane, and e is the first detection line, and h is the second detection
Line, f is the first control line, and i is the second control line, and g is absorbent filter；
Fig. 2 is the top view after the combination of described combined two-way colloidal gold immuno-chromatography test paper strip, and in this figure, letter refers to and schemes
Described in 1 unanimously.
Detailed description of the invention
For making the purpose of this utility model, technical scheme and advantage clearer, below in conjunction with accompanying drawing to reality of the present utility model
The mode of executing is explained in detail.But, it will be understood by those skilled in the art that in this utility model embodiment,
In order to make reader be more fully understood that, the application proposes many ins and outs.But, even if do not have these ins and outs and based on
The many variations of implementation below and amendment, it is also possible to realize the application each claim technical scheme required for protection.
First embodiment of the present utility model relates to a kind of colloidal gold immuno-chromatography test paper strip, comprises: a is sample pad, and b is glue
Body gold pad, c is a liner plate in two groups, and d is nitrocellulose membrane, and e is the first detection line, and h is the second detection line, f
Being the first control line, i is the second control line, and g is absorbent filter, as shown in Figure 1.
In Fig. 1, the assembly that right side does not indicates is and left side symmetrical structure.Individually gold colloidal knot is pasted in the end above end liner c
Closing pad d, end liner c end pastes adsorptive pads g；Gold conjugation pad b is pasted at middle part above gold conjugation pad d；Sample
Product pad a blocks with gold conjugation pad b phase and is connected.And by the shell of colloidal gold immuno-chromatography test paper strip compress adsorptive pads g and
Sample pad a.
Wherein, the bottom of gold conjugation pad b is polyester fiber film, has gold colloidal albumen in this polyester fiber film surface adsorption；
The bottom of gold conjugation pad d is polyester fiber film, has antigen or antibody protein at this polyester fiber film pan coating；Sample pad
The material of a is polyester fiber film；The material of adsorptive pads g is papery complex fiber material；The material of end liner c is polyvinyl chloride fibre
Or glass fibre.The preparation method of the combined two-way colloidal gold immuno-chromatography test paper strip in present embodiment is standby liner plate card
Button agrees with sample pad draw-in groove, selects two groups of liner plates, can be connected by draw-in groove with sample pad from arbitrarily two or more sets liner plates.
The using method of the colloidal gold immuno-chromatography test paper strip of present embodiment is as follows: dripped in sample pad a by sample solution,
Sample solution, through the permeation filtration of sample pad a, is diffused on gold conjugation pad b, owing to both sides gold conjugation pad is tight
Buckle is connected, and both sides gold conjugation pad is homogeneous with the substrate of sample pad a, fiber intercommunication.Gold albumen is uniformly to two boundary layers
Analysis diffusion, present embodiment is by being layered gold conjugation pad, the gold conjugation pad of absorption gold colloidal albumen and sample pad
It is arranged at the middle part of end liner, and adsorptive pads is arranged at the two ends of end liner so that colloidal gold immuno-chromatography test paper strip can bidirectional layer
Analysis.And often it is stored in refrigerator by substrate, immediately use, such that it is able to increase the stability of colloidal gold chromatographic, improve work efficiency.
Claims (8)
1. a combined two-way colloidal gold immuno-chromatography test paper strip, it is characterised in that agent structure comprises: liner plate and sample pad；
Described liner plate is the independent two group liner plates with buckle, and the assembly often pasted on group liner plate includes: gold conjugation pad,
Nitrocellulose membrane, absorbent filter；
Described sample pad includes that material is fixed in sample absorbing material and bottom, and material institute is fixed in sample absorbing material and bottom
The draw-in groove constituted.
Combined two-way colloidal gold immuno-chromatography test paper strip the most according to claim 1, it is characterised in that described sample
It is polyester fiber film that the raw material of material is fixed in the absorbing material of pad and bottom.
Combined two-way colloidal gold immuno-chromatography test paper strip the most according to claim 1, it is characterised in that gold colloidal is tied
The bottom closing pad is polyester fiber film, has gold colloidal albumen in this polyester fiber film surface adsorption.
Combined two-way colloidal gold immuno-chromatography test paper strip the most according to claim 1, it is characterised in that described nitric acid
Fibrous membrane pan coating has antigen or antibody protein.
Combined two-way colloidal gold immuno-chromatography test paper strip the most according to claim 1, it is characterised in that described sample
Pad is connected with the liner plate buckle of both direction by draw-in groove.
Combined two-way colloidal gold immuno-chromatography test paper strip the most according to claim 4, it is characterised in that described nitric acid
Envelope antigen on fibrous membrane or antibody protein are positioned at the two ends of film.
Combined two-way colloidal gold immuno-chromatography test paper strip the most according to claim 6, it is characterised in that at described nitre
The two ends of acid fibrous membrane, are respectively present antigen described at least one or antibody protein.
Combined two-way colloidal gold immuno-chromatography test paper strip the most according to claim 1, it is characterised in that standby liner plate
Buckle with on liner plate with buckle identical, standby liner plate buckle agrees with sample pad draw-in groove, from any two or more sets liner plates
Select two groups of liner plates, can be connected by draw-in groove with sample pad.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620065094.5U CN205484358U (en) | 2016-01-20 | 2016-01-20 | Combined two -way colloidal gold immunoassay chromatography test paper strip |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620065094.5U CN205484358U (en) | 2016-01-20 | 2016-01-20 | Combined two -way colloidal gold immunoassay chromatography test paper strip |
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| CN205484358U true CN205484358U (en) | 2016-08-17 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107402302A (en) * | 2017-08-06 | 2017-11-28 | 潘荣兰 | A kind of environmental monitoring prokaryotes quantity rapid evaluation chip |
| CN107656050A (en) * | 2017-10-07 | 2018-02-02 | 贾晓轻 | Blood sample immunochromatography diagnosis test paper and kit |
| CN107860917A (en) * | 2017-11-11 | 2018-03-30 | 贾晓轻 | Immunochromatography diagnosis test paper and kit |
| CN113758915A (en) * | 2021-04-23 | 2021-12-07 | 威海纽普生物技术有限公司 | Novel lateral chromatography detection card |
| CN115166238A (en) * | 2022-08-02 | 2022-10-11 | 中国农业科学院北京畜牧兽医研究所 | Integrated antibody detection test strip for primary screening and accurate diagnosis of brucellosis in sheep |
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2016
- 2016-01-20 CN CN201620065094.5U patent/CN205484358U/en active Active
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107402302A (en) * | 2017-08-06 | 2017-11-28 | 潘荣兰 | A kind of environmental monitoring prokaryotes quantity rapid evaluation chip |
| CN107656050A (en) * | 2017-10-07 | 2018-02-02 | 贾晓轻 | Blood sample immunochromatography diagnosis test paper and kit |
| CN107656050B (en) * | 2017-10-07 | 2019-06-04 | 南京鼎晶生物科技有限公司 | Blood sample immunochromatography diagnosis test paper and kit |
| CN107860917A (en) * | 2017-11-11 | 2018-03-30 | 贾晓轻 | Immunochromatography diagnosis test paper and kit |
| CN107860917B (en) * | 2017-11-11 | 2019-07-12 | 山东朱氏药业集团有限公司 | Immunochromatography diagnosis test paper and kit |
| CN113758915A (en) * | 2021-04-23 | 2021-12-07 | 威海纽普生物技术有限公司 | Novel lateral chromatography detection card |
| CN115166238A (en) * | 2022-08-02 | 2022-10-11 | 中国农业科学院北京畜牧兽医研究所 | Integrated antibody detection test strip for primary screening and accurate diagnosis of brucellosis in sheep |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20210602 Address after: Room 1410, Jingkai building, 1000 Yongxing Road, Zhongdai street, Pinghu City, Jiaxing City, Zhejiang Province, 314200 Patentee after: Zhejiang Hengpu Pharmaceutical Co.,Ltd. Address before: 210014 4th floor, building B, 8 Yongfeng Avenue, Baixia high tech Industrial Park, Qinhuai District, Nanjing City, Jiangsu Province Patentee before: HOPE ANALYTECH Inc. |
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| TR01 | Transfer of patent right |