CN205384281U - A trapezoidal chromatography test paper strip for detecting multicomponent pesticide residue - Google Patents
A trapezoidal chromatography test paper strip for detecting multicomponent pesticide residue Download PDFInfo
- Publication number
- CN205384281U CN205384281U CN201520981754.XU CN201520981754U CN205384281U CN 205384281 U CN205384281 U CN 205384281U CN 201520981754 U CN201520981754 U CN 201520981754U CN 205384281 U CN205384281 U CN 205384281U
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- Prior art keywords
- trapezoidal
- gold
- test strip
- pad
- chromatograph test
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- 239000000447 pesticide residue Substances 0.000 title claims abstract description 13
- 238000004587 chromatography analysis Methods 0.000 title abstract description 7
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims abstract description 46
- 229910052737 gold Inorganic materials 0.000 claims description 33
- 239000010931 gold Substances 0.000 claims description 33
- 238000001514 detection method Methods 0.000 claims description 23
- 239000000020 Nitrocellulose Substances 0.000 claims description 15
- 229920001220 nitrocellulos Polymers 0.000 claims description 15
- 230000021615 conjugation Effects 0.000 claims description 13
- 239000000835 fiber Substances 0.000 claims description 12
- 239000012528 membrane Substances 0.000 claims description 12
- 229920000728 polyester Polymers 0.000 claims description 11
- 239000000427 antigen Substances 0.000 claims description 10
- 102000036639 antigens Human genes 0.000 claims description 10
- 108091007433 antigens Proteins 0.000 claims description 10
- 239000002250 absorbent Substances 0.000 claims description 9
- 230000002745 absorbent Effects 0.000 claims description 9
- 102000004169 proteins and genes Human genes 0.000 claims description 9
- 108090000623 proteins and genes Proteins 0.000 claims description 9
- 238000002372 labelling Methods 0.000 claims description 8
- ZBKIUFWVEIBQRT-UHFFFAOYSA-N gold(1+) Chemical compound [Au+] ZBKIUFWVEIBQRT-UHFFFAOYSA-N 0.000 claims description 7
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 239000011248 coating agent Substances 0.000 claims description 3
- 238000000576 coating method Methods 0.000 claims description 3
- 238000001179 sorption measurement Methods 0.000 claims description 3
- 239000002994 raw material Substances 0.000 claims description 2
- 238000003018 immunoassay Methods 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 29
- 239000000084 colloidal system Substances 0.000 description 16
- 239000003153 chemical reaction reagent Substances 0.000 description 9
- 238000000034 method Methods 0.000 description 8
- 239000000463 material Substances 0.000 description 7
- 238000003317 immunochromatography Methods 0.000 description 5
- 239000011358 absorbing material Substances 0.000 description 4
- 238000010186 staining Methods 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 2
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000000274 adsorptive effect Effects 0.000 description 2
- 238000011091 antibody purification Methods 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 239000012488 sample solution Substances 0.000 description 2
- 229920002160 Celluloid Polymers 0.000 description 1
- 238000005411 Van der Waals force Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 150000001793 charged compounds Chemical class 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 238000001493 electron microscopy Methods 0.000 description 1
- 239000002657 fibrous material Substances 0.000 description 1
- 239000003365 glass fiber Substances 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000003119 immunoblot Methods 0.000 description 1
- 238000000148 multi-dimensional chromatography Methods 0.000 description 1
- 238000004375 physisorption Methods 0.000 description 1
- 229920000915 polyvinyl chloride Polymers 0.000 description 1
- 239000004800 polyvinyl chloride Substances 0.000 description 1
- 239000000700 radioactive tracer Substances 0.000 description 1
- 230000011218 segmentation Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 238000007447 staining method Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
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- Peptides Or Proteins (AREA)
Abstract
The utility model provides a middle part that a trapezoidal chromatography test paper strip for detecting multicomponent pesticide residue lies in especially that the even step of golden albumen spreads, the colloidal gold that combines the colloidal gold to fill up, adsorb colloidal gold albumen combines pad and the layering of sample pad to set up in the end liner to to absorb water to fill up sets up in the end of end liner, makes the colloidal gold immunoassay chromatography test paper strip can the step chromatography.
Description
Technical field
This utility model relates to a kind of trapezoidal chromatograph test strip for detecting multicomponent pesticide residues, for sample be likely to containing main three to the plurality of target of difference, the instruction of different step chromatography can be monitored, belong to analytical chemistry field.
Background technology
Gold colloidal is a kind of conventional labelling technique, is a kind of novel immunolabelling technique being applied to antigen-antibody using gold colloidal as tracer label thing, the advantage having its uniqueness.In recent years widely use in various biological studies.Immunoblot assay at Clinical practice nearly all uses its labelling.
What the biomacromolecules such as colloid gold label substantially antibody protein were adsorbed to colloid gold particle surface is coated process.The mechanism of absorption is probably between negative charge that gold colloid surface is with and the positive charge that protein molecule is with and attracts each other by electrostatic force, reaches namely to be formed in Van der Waals force firmly to combine.It addition, the coarse of colloid gold particle is also advantageous that in the essential condition forming absorption.Due to this labeling process mainly by physisorption, thus the biologic activity of protein molecule is had not significant impact.
Conventional immune colloid gold detection technique and application include: (1) immune colloid gold light microscopic staining cell suspension smear or tissue slice, the antibody of available colloid gold label dyes, also can on the basis of colloid gold label, labelling is strengthened with silver developer solution, make the silver atoms being reduced be deposited on the gold grain surface of labelling, the sensitivity of colloid gold label can be remarkably reinforced.(2) immune colloid gold staining method for electron microscopy, the antibody of available colloid gold label or anti antibody are combined with negative staining Virus Sample or tissue ultrathin section, then carry out negative staining.Can be used for the observation of morphology of virus and Viral diagnosis.(3) dot immunogold filtration assay, carrier is made in application microporous filter membrane (such as film), first by antigen or antibody point on film, adds sample to be checked after closing, with the corresponding antigen of antibody test of colloid gold label or antibody after washing.(4) colloidal gold immunity chromatography, specific antigen or antibody are fixed on film with ribbon, colloid gold label reagent (antibody or monoclonal antibody) is adsorbed on pad, after in the sample pad that sample to be checked is added to test strips one end, moved forward by capillarity, react to each other after dissolving the colloid gold label reagent on pad, when moving the region to fixing antigen or antibody again, the conjugate of thing to be checked and gold marked reagent occurs with it again specific binding and is trapped, it is gathered on detection band, can pass through to be observed visually colour developing result.Above-mentioned four kinds of methods have progressively developed into diagnosis test paper, and prospect is extensive.
The assembling process of tradition gold colloidal quick measuring card is for being fixed in reagent draw-in groove lower house using polrvinyl chloride liner plate as supporting carrier, then sample pad, composite colloid gold pad, nitrocellulose filter and absorbent filter are arranged in order and are connected to upper surface on the right side of liner plate, and single gold conjugation pad, nitrocellulose filter and absorbent filter are arranged in order and are connected to the upper surface of liner plate on the left of sample pad and obtain colloidal gold immunochromatographimethod quick measuring card.Except conventional detection labelled reagent bar, generally after clearly detecting target, then assemble colloid gold reagent bar.And the general component of pesticide residues is more, when multicomponent analysis, inefficiency.One has trapezoidal, multicomponent labelling quick detection reagent bar and does not have been reported that at present.
Summary of the invention
This utility model describes a kind of trapezoidal chromatograph test strip for detecting multicomponent pesticide residues, can for sample be likely to containing different main plurality of target, select the liner plate of different directions to form multidimensional chromato-graphy reagent strip with sample pad combination.Agent structure comprises: 1 is sample pad, and 2 is gold conjugation pad, and 3 is liner plate, and 4 is nitrocellulose membrane, and 5 is detection line gradient, and 6 is control line, and 7 is absorbent filter.Above-mentioned each test strips is the independent liner plate with buckle, is provided with detection line and control line, gold conjugation pad, nitrocellulose membrane, absorbent filter separately above；Described sample pad includes the fixing material of sample absorbing material and bottom and the draw-in groove that the fixing material of sample absorbing material and bottom is constituted.Immobilized in described detection line gradient have detection line, and detection line number is 2-6, and described trapezoidal chromatograph test strip labelling is no less than 2 kinds of markers.
A kind of trapezoidal chromatograph test strip for detecting multicomponent pesticide residues, its agent structure comprises: liner plate and sample pad, and described liner plate and sample pad are stand-alone assembly, and described liner plate is the stand-alone assembly assembled, and is generally stored in less than 15 DEG C Refrigerator stores.Described liner plate combined method but be not limited to: using polrvinyl chloride collet as support carrier be fixed in reagent draw-in groove lower house, then sample pad, composite colloid gold pad, nitrocellulose filter and absorbent filter are arranged in order and are connected to liner plate upper surface.Described liner plate is two groups of independent liner plates with buckle, and often on group liner plate, the assembly pasted includes: gold conjugation pad, nitrocellulose membrane, absorbent filter and or other the art staff understandable.The stability of colloidal sol is between small molecular ion solution and rough segmentation dephasing, and its granule does Brownian movement, is not easily affected by gravity and sinks.
Described sample pad includes the fixing material of sample absorbing material and bottom and the draw-in groove that the fixing material of sample absorbing material and bottom is constituted.The raw material of described sample pad is polyester fiber film.Described multidirectional colloidal gold immuno-chromatography test paper strip, the bottom of gold conjugation pad is polyester fiber film, has gold colloidal albumen in this polyester fiber film surface adsorption, and described nitrocellulose membrane pan coating has antigen or antibody protein.Described sample pad is connected with the liner plate buckle of both direction by draw-in groove.Envelope antigen on described nitrocellulose membrane or antibody protein are positioned at the two ends of film.Described multidirectional colloidal gold immuno-chromatography test paper strip, at the two ends of described nitrocellulose membrane, is respectively present antigen described at least one or antibody protein.Nitrocellulose filter is provided with the first detection line and the first control line, celluloid is provided with the second detection line and the second control line, coated on first detection line is first object antibody, coated on second detection line is the second target antibody, coated on first control line is first object antibody purification, coated on second control line is the second target antibody purification, the suitable package amount of antibody respectively 1.0-2.0 μ g, and compound gold mark probe suitable antibodies labelled amount is 10-20 μ g/mL.
Described multidirectional colloidal gold immuno-chromatography test paper strip, standby liner plate buckle and sample pad draw-in groove agree with, and can be connected by draw-in groove with sample pad.
Accompanying drawing explanation
In the following description to embodiment, the present invention will become apparent to, and only passes through embodiment and is described with reference to the drawings, wherein:
Fig. 1 is the profile of described trapezoidal chromatograph test strip, and wherein 1 is sample pad, and 2 is gold conjugation pad, and 3 is liner plate, and 4 is nitrocellulose membrane, and 5 is detection line gradient, and 6 is control line, and 7 is absorbent filter.
Detailed description of the invention
For making the purpose of this utility model, technical scheme and advantage clearly, below in conjunction with accompanying drawing, embodiment of the present utility model is explained in detail.But, it will be understood by those skilled in the art that in this utility model embodiment, propose many ins and outs in order to make reader be more fully understood that the application.But, even without these ins and outs with based on the many variations of implementation below and amendment, it is also possible to realize the application each claim technical scheme required for protection.
Fig. 1 is the profile of described trapezoidal chromatograph test strip, and wherein 1 is sample pad, and 2 is gold conjugation pad, and 3 is liner plate, and 4 is nitrocellulose membrane, and 5 is detection line gradient, and 6 is control line, and 7 is absorbent filter.8 is the first detection line, 9 be the second detection line, 10 be the 3rd detection line, 11 be the 4th detection line.
Wherein, gold conjugation pad bottom is polyester fiber film, has gold colloidal albumen in this polyester fiber film surface adsorption；The bottom of gold conjugation pad is polyester fiber film, has antigen or antibody protein at this polyester fiber film pan coating；The material of sample pad is polyester fiber film；The material of adsorptive pads is papery complex fiber material；The material of end liner is polyvinyl chloride fibre or glass fibre.
The using method of the colloidal gold immuno-chromatography test paper strip of present embodiment is as follows: being dripped in sample pad 1 by sample solution, sample solution is through the permeation filtration of pad.The uniform step diffusion of gold albumen, present embodiment by being arranged at the middle part of end liner by gold conjugation pad, the gold conjugation pad adsorbing gold colloidal albumen and sample pad layering, and adsorptive pads is arranged at the end of end liner so that colloidal gold immuno-chromatography test paper strip can step chromatography.And often it is stored in refrigerator by substrate, immediately use, such that it is able to increase the stability of colloidal gold chromatographic, improve work efficiency.
Claims (6)
1. the trapezoidal chromatograph test strip being used for detecting multicomponent pesticide residues, it is characterized in that, agent structure comprises: sample pad (1), gold conjugation pad (2), liner plate (3), nitrocellulose membrane (4), detection line gradient (5), control line (6), absorbent filter (7)；Immobilized in described detection line gradient have detection line, and detection line number is 2-6.
2. the trapezoidal chromatograph test strip for detecting multicomponent pesticide residues according to claim 1, it is characterised in that the raw material of described sample pad is polyester fiber film.
3. the trapezoidal chromatograph test strip for detecting multicomponent pesticide residues according to claim 1, it is characterised in that the bottom of gold conjugation pad is polyester fiber film, has gold colloidal albumen in this polyester fiber film surface adsorption.
4. the trapezoidal chromatograph test strip for detecting multicomponent pesticide residues according to claim 1, it is characterised in that described nitrocellulose membrane pan coating has antigen or antibody protein.
5. the trapezoidal chromatograph test strip for detecting multicomponent pesticide residues according to claim 4, it is characterised in that envelope antigen on described nitrocellulose membrane or antibody protein are positioned at the two ends of film.
6. the trapezoidal chromatograph test strip for detecting multicomponent pesticide residues according to claim 1, it is characterised in that described trapezoidal chromatograph test strip labelling is no less than 2 kinds of markers.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520981754.XU CN205384281U (en) | 2015-11-30 | 2015-11-30 | A trapezoidal chromatography test paper strip for detecting multicomponent pesticide residue |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520981754.XU CN205384281U (en) | 2015-11-30 | 2015-11-30 | A trapezoidal chromatography test paper strip for detecting multicomponent pesticide residue |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205384281U true CN205384281U (en) | 2016-07-13 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520981754.XU Active CN205384281U (en) | 2015-11-30 | 2015-11-30 | A trapezoidal chromatography test paper strip for detecting multicomponent pesticide residue |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205384281U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111707819A (en) * | 2020-06-22 | 2020-09-25 | 上海雄图生物科技有限公司 | AFB1, ZEN and DON three-index quantitative chromatography test strip |
| CN113758915A (en) * | 2021-04-23 | 2021-12-07 | 威海纽普生物技术有限公司 | Novel lateral chromatography detection card |
-
2015
- 2015-11-30 CN CN201520981754.XU patent/CN205384281U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111707819A (en) * | 2020-06-22 | 2020-09-25 | 上海雄图生物科技有限公司 | AFB1, ZEN and DON three-index quantitative chromatography test strip |
| CN113758915A (en) * | 2021-04-23 | 2021-12-07 | 威海纽普生物技术有限公司 | Novel lateral chromatography detection card |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| DD01 | Delivery of document by public notice |
Addressee: Sun Shouchen Document name: payment instructions |
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| DD01 | Delivery of document by public notice | ||
| DD01 | Delivery of document by public notice |
Addressee: Sun Shouchen Document name: Notice of Approval for Restoration of Rights Request |
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