CN207081742U - A kind of ABO/Rh blood groups diagnose test card - Google Patents
A kind of ABO/Rh blood groups diagnose test card Download PDFInfo
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- CN207081742U CN207081742U CN201720960464.6U CN201720960464U CN207081742U CN 207081742 U CN207081742 U CN 207081742U CN 201720960464 U CN201720960464 U CN 201720960464U CN 207081742 U CN207081742 U CN 207081742U
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- blood
- abo
- flushing
- monoclonal antibody
- test strips
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Abstract
The utility model provides a kind of ABO/Rh blood groups diagnosis test card, including the test strips being bonded on bottom plate and bottom plate, the test strips are provided with multiple rinsing areas, and along corresponding with each rinsing area multiple loading areas in chromatography direction, the solid phase monoclonal antibody of anti-A, B or Rh blood group antigens is contained in the loading area respectively, for adding the interpretation of blood sample and Blood grouping result;The rinsing area is used to add flushing liquor, and promoting blood sample, the flushing liquor includes 3 kinds of flushing liquors of the Nano-Au probe of the labeling of monoclonal antibody of anti-A, B or Rh blood group antigens, for marking A types, Type B or RH positive red blood cells respectively along the flowing of chromatography direction.The utility model product can synchronously detect ABO/Rh blood groups;It is small volume, in light weight, it is easy to carry about with one;Without external power supply or other laboratory equipments;As a result accurately and reliably, there is higher Sensitivity and Specificity;Operating method is simple, is as a result easy to judge, even if layman can also be used.
Description
Technical field
The utility model belongs to technical field of medical examination, more particularly to a kind of ABO/Rh blood groups diagnosis test card.
Background technology
Clinical conventional ABO/Rh blood type testing methods have slide method, test tube method and micro-column gel agglutination assay etc. at present.With top
Method is accurate, objective, has the Sensitivity and Specificity of height, but still have weak point:(1) professional is needed to operate;(2) spy is needed
Fixed laboratory apparatus;(3) suitable experiment condition is needed;(4) reaction time is longer;(5) blood volume needed is more;(6) sample needs
Want pre-treatment.Therefore, though traditional blood type testing methods can meet the needs of clinical and laboratory, battlefield ring can not be met
The needs for layman's detections such as the special circumstances such as border, major natural disasters and blood sampling are live, family uses.Therefore, valency
Lattice are cheap, it is simple and efficient, be easy to carry about with one, visual result, the real-time test (point of care test, POCT) for being easy to interpretation
Method is most important to the Blood grouping of special circumstances.
The content of the invention
For drawbacks described above of the prior art, main purpose of the present utility model is that providing a kind of ABO/Rh blood groups examines
Disconnected test card, it can synchronously detect ABO/Rh blood groups, and be easy to carry about with one, operating method it is simple, be as a result easy to judge etc. excellent
Point.
In order to achieve the above object, the utility model adopts the following technical scheme that:A kind of ABO/Rh blood groups diagnose test card,
Including the test strips being bonded on bottom plate and bottom plate, the test strips are provided with multiple rinsing areas, and along chromatography direction and respectively
The solid phase monoclonal antibody of anti-A, B or Rh blood group antigens is contained in multiple loading areas corresponding to individual rinsing area, the loading area respectively,
For adding the interpretation of blood sample and Blood grouping result;The rinsing area is used to add flushing liquor, promotes blood sample
Along the flowing of chromatography direction, the flushing liquor includes the 3 of the Nano-Au probe of the labeling of monoclonal antibody of anti-A, B or Rh blood group antigens
Kind flushing liquor, for marking A types, Type B or RH positive red blood cells respectively.
Preferably the test strips are 3 be set up in parallel, and its length direction is consistent with chromatographing direction as further,
Rinsing area and loading area are disposed with along chromatography direction in each test strips, different blood group antigens are contained in each loading area
Solid phase monoclonal antibody, the loading area is used to add the interpretation of blood sample and Blood grouping result, each punching
Wash area to be used to add flushing liquor, promote blood sample to include anti-A, B or Rh blood group antigens along the flowing of chromatography direction, the flushing liquor
Labeling of monoclonal antibody Nano-Au probe 3 kinds of flushing liquors, for marking A types, Type B and RH positive red blood cells respectively.
As the space for further preferably, also including a panel, the bottom plate and panel formation above the test strips
The test strips are fixed on interior;Chromatography direction is provided with multiple flushing holes on the panel, and along chromatography direction and respectively
Multiple loading holes corresponding to individual flushing hole, the flushing hole are used to add the monoclonal antibody for including anti-A, B or Rh blood group antigens
The flushing liquor of the Nano-Au probe of mark, the loading hole are used to add blood sample to be detected.
As further preferably, the flushing hole and loading hole are respectively 3.
As it is further preferably, the bottom plate and panel is have hard paper, machine glass, polystyrene, polycarbonate-based
Transparent or opaque plastics.
Adsorptive pads are also preferably bonded with the bottom plate as further, positioned at the end in chromatography direction, with the examination
Paper slip lapping ends.
As further preferably, the adsorptive pads are absorbent filter.
The beneficial effects of the utility model are:The utility model ABO/Rh blood groups diagnose test card, including bottom plate and bottom
The test strips being bonded on plate, the test strips are provided with multiple rinsing areas, and corresponding with each rinsing area along chromatography direction
Multiple loading areas, the solid phase monoclonal antibody of anti-A, B or Rh blood group antigens is contained in the loading area respectively, for adding blood sample
The interpretation of product and Blood grouping result;The rinsing area is used to add flushing liquor, promotes blood sample to be flowed along chromatography direction,
The flushing liquor includes 3 kinds of flushing liquors of the Nano-Au probe of the labeling of monoclonal antibody of anti-A, B or Rh blood group antigens, can distinguish
Mark A types, Type B or RH positive red blood cells.Blood sample to be detected is added in each loading area when the utility model detects, then in each punching
Wash area and be separately added into different flushing liquors, the labeling of monoclonal antibody mistake of anti-A, B or Rh blood group antigens is included in the flushing liquor
Nanogold particle, the flushing liquor moves along chromatography direction, the erythrocyte binding of blood group corresponding to blood sample to be detected and
Loading area develops the color, such as when the flushing liquor is the nanogold particle that the labeling of monoclonal antibody comprising anti-Blood group antigen A is crossed,
If blood sample to be detected is A types, the A types antigen of the blood sample to be detected then monoclonal with the anti-Blood group antigen A on nanogold particle
Antibody is specifically bound in loading area, so as to which red blood cell fixes colour developing, if not A types do not develop the color then in loading area, it is other
Blood group is similar, thus the utility model can detect A, B, Rh blood group simultaneously.
Brief description of the drawings
, below will be to embodiment in order to illustrate more clearly of the utility model embodiment or technical scheme of the prior art
In the required accompanying drawing used be briefly described, it should be apparent that, drawings in the following description are only of the present utility model
Some embodiments, for those of ordinary skill in the art, on the premise of not paying creative work, can also be according to this
A little accompanying drawings obtain other accompanying drawings.
Fig. 1 is the structural representation that the blood group of the utility model embodiment 1 diagnoses test card.
Fig. 2 is the structural representation that the blood group of the utility model embodiment 2 diagnoses test card.
Fig. 3 a-3h are the testing result schematic diagram that the blood group of the utility model embodiment 2 diagnoses test card.
What is marked in accompanying drawing is described as follows:1- bottom plates, 2.1,2.2,2.3- test strips, D- rinsing areas, S- loadings area, 3- faces
Plate, D1, D2, D3- flushing hole, S1, S2, S3- loading hole.
Embodiment
The utility model diagnoses test card by providing a kind of ABO/Rh blood groups, solves traditional blood type testing methods not
It can meet that the laymans such as the special circumstances such as battlefield environment, major natural disasters and blood sampling are live, family uses detect need
The defects of wanting.
In order to solve drawbacks described above, the main thought of the utility model embodiment is:
The test strips being bonded on the utility model embodiment ABO/Rh blood groups diagnosis test card, including bottom plate and bottom plate,
The test strips are provided with multiple rinsing areas, and along chromatography direction multiple loading areas corresponding with each rinsing area, it is described on
The solid phase monoclonal antibody of anti-A, B or Rh blood group antigens is contained in sample area respectively, for adding blood sample and Blood grouping knot
The interpretation of fruit;The rinsing area is used to add flushing liquor, promotes blood sample to include anti-along the flowing of chromatography direction, the flushing liquor
A, 3 kinds of flushing liquors of the Nano-Au probe of the labeling of monoclonal antibody of B or Rh blood group antigens, for mark respectively A types, Type B or
RH positive red blood cells.
Nanogold, that is, refer to golden molecule of the diameter in 1-100nm, have high electron density, catalytic action and dielectric special
Property, it can be combined with a variety of large biological molecules, and do not influence its biological activity.Immunological probe mark skill based on nanogold
Art be using nanogold as probe material be applied to antigen-antibody between specific reaction a kind of novel immune labelling technique, be after
Enzyme is immune labeled, prominent light is immune labeled and isotope it is immune labeled after novel immune labelling technique.This labelling technique is not
Only have the advantages that it is accurate, simple, quick and pollution-free, and independent of costliness detecting instrument, can with the naked eye be distinguished
Not, it is particularly suitable for vast grass-roots unit, hospital and generaI investigation etc. on a large scale.
The base fluid of the flushing liquor may be selected from:Citric acid-sodium citrate buffer solution, citric acid-sodium hydroxide-hydrochloride buffer
Liquid, phthalic acid-hydrochloride buffer, disodium hydrogen phosphate-citrate buffer solution, acetic acid-sodium acetate buffer solution, phthalic acid
Hydrogen potassium-sodium hydrate buffer solution, glycine-HCI buffer solution etc., or their dilution.
The utility model embodiment is by the use of preparing Nano-Au probe as detection tracer, and different monoclonal cell sources
Anti- A, B, Rh blood group antigens insolubilized antibody of the mouse monoclonal antibody as detection, developing can joint-detection ABO/Rh blood
The quick diagnosis test card of type, it is allowed to obtain satisfied sensitivity, specificity, repeatability, stability.
The utility model embodiment can synchronously detect ABO/Rh blood groups;It is small volume, in light weight, it is easy to carry about with one;Without outer
Connect power supply or other laboratory equipments;As a result accurately and reliably, there is higher Sensitivity and Specificity;Operating method is simple, knot
Fruit is easy to judge, even if layman can also be used.It is thus special particularly suitable for battlefield surroundings and major natural disasters etc.
Under environment, layman carries out blood group quick detection to the wounded, while the utility model embodiment product can also meet to adopt confession
Simple, the quick and free of contamination demand of blood mechanism, army, basic hospital and domestic consumer to Blood grouping.
Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the embodiment of the utility model is carried out
Clearly and completely describing, it is clear that described embodiment is only the utility model part of the embodiment, rather than whole
Embodiment.Based on the embodiment in the utility model, every other embodiment that those of ordinary skill in the art are obtained all belongs to
In the scope of the utility model protection.
Embodiment 1
The murine monoclonal hybridoma for expressing anti-A, B or Rh blood group antigens specific antibody is prepared using monoclonal antibody technique
Cell, and with ascites method prepare, collect monoclonal antibody.
Using Nano-Au probe labelling technique, the monoclonal antibody of anti-A, B or Rh blood group antigens is entered to nanogold particle
Line flag, so as to obtain the Nano-Au probe that can detect A, B or Rh blood group antigens.
As shown in figure 1, three be bonded on the present embodiment ABO/Rh blood groups diagnosis test card, including bottom plate 1 and bottom plate 1
Test strips (2.1,2.2,2.3), respectively A types test strips, Type B test strips and Rh type test strips, the length side of three test strips
To consistent with chromatography direction, and it is set up in parallel;In each test strips rinsing area D and loading area S is disposed with along chromatography direction;
Loading area S is used to add detected blood 1-2 drops, and carries out the interpretation of blood group antigens result;
The loading area S of wherein A types test paper contains the solid phase monoclonal antibody of anti-Blood group antigen A, the loading area S of Type B test paper
Solid phase monoclonal antibody containing anti-Blood group antigen B, the loading area S of Rh type test paper contain the solid phase monoclonal of anti-Rh blood group antigens
Antibody;Each rinsing area D is used to add flushing liquor, for promoting blood sample along the flowing of chromatography direction, the flushing liquor
3 kinds of flushing liquors of the Nano-Au probe of the labeling of monoclonal antibody comprising anti-A, B or Rh blood group antigens, can mark A types, B respectively
Type and RH positive red blood cells.The A types test paper flushing liquor of the Nano-Au probe of the labeling of monoclonal antibody of anti-Blood group antigen A, B
The flushing liquor of the type test paper Nano-Au probe of the labeling of monoclonal antibody of anti-Blood group antigen B, Rh types test paper are resisted with anti-Rh blood groups
The flushing liquor of the Nano-Au probe of former labeling of monoclonal antibody.
During detection, blood sample to be detected is first added dropwise respectively in the respective loading area of three test strips (2.1,2.2,2.3), then
Respectively test strips 2.1 rinsing area add comprising anti-A type antigen labeling of monoclonal antibody Nano-Au probe flushing liquor,
The flushing liquor of the Nano-Au probe of the labeling of monoclonal antibody comprising anti-Type B antigen is added in the rinsing area of test strips 2.2, and
The flushing of the Nano-Au probe of the labeling of monoclonal antibody of the anti-D antigens comprising Rh blood groups is added in the rinsing area of test strips 2.3
Liquid.When each flushing liquor edge chromatography direction of above-mentioned rinsing area is moved to loading area, corresponding blood group is red thin to blood sample to be detected
Born of the same parents combine and developed the color, such as:When the nanogold particle that the labeling of monoclonal antibody that the flushing liquor includes anti-Blood group antigen B is crossed
When, if blood sample to be detected is Type B, the Dan Ke of the Type B antigen and the anti-Blood group antigen B on nanogold particle of blood sample to be detected
Grand antibody is specifically bound, and is developed the color so as to be fixed in loading area red blood cell, if not Type B, then do not develop the color in loading area.Its
His blood group is similar.Thus the present embodiment can detect A, B, Rh blood group simultaneously, and naked eyes are visible.
Certainly, the type and priority addition sequence of the addition monoclonal antibody of above-mentioned test strips 2.1,2.2 and 2.3
With change.
The base fluid of the flushing liquor is phthalic acid-hydrochloride buffer, and pH value 4.2, dosage is 50 μ l.
Above-mentioned bottom plate 1 is polystyrene plastics.
Embodiment 2
The murine monoclonal hybridoma for expressing anti-A, B or Rh blood group antigens specific antibody is prepared using monoclonal antibody technique
Cell, and with ascites method prepare, collect monoclonal antibody.
Using Nano-Au probe labelling technique, the monoclonal antibody of anti-A, B or Rh blood group antigens is entered to nanogold particle
Line flag, so as to obtain the Nano-Au probe that can detect A, B or Rh blood group antigens.
As shown in Fig. 2 the present embodiment ABO/Rh blood groups diagnose test card, including bottom plate 1, panel 3 and in bottom plate 1 and face
Three test strips (2.1,2.2,2.3) being adhesively fixed between plate 3, the length direction of three test strips is consistent with chromatography direction,
And it is set up in parallel;Chromatography direction is provided with multiple flushing hole D1, D2, D3 on the panel 3, and along chromatography direction with it is each
Multiple loading hole S1, S2, S3 corresponding to flushing hole, described flushing hole D1, D2, D3 are used to be separately added into comprising anti-A, B or Rh blood
The flushing liquor of the Nano-Au probe of the labeling of monoclonal antibody of type antigen, described loading hole S1, S2, S3 are to be checked for being separately added into
Survey blood sample and result interpretation.
During detection, the first respective loading hole (S1, S2, S3) in three test strips (2.1,2.2,2.3) is added dropwise to be checked respectively
Blood sample is surveyed, then adds the nanogold of the labeling of monoclonal antibody comprising anti-A type antigen in the flushing hole D1 of test strips 2.1 respectively
The flushing liquor of probe, test strips 2.2 flushing hole D2 add comprising anti-Type B antigen labeling of monoclonal antibody nanogold visit
The flushing liquor of pin, and add the labeling of monoclonal antibody of the anti-D antigens comprising Rh blood groups in the flushing hole D3 of test strips 2.3
The flushing liquor of Nano-Au probe.Above-mentioned each flushing liquor moves along chromatography direction, the red blood cell of blood group corresponding to blood sample to be detected
With reference to and develop the color, such as:When the nanogold particle that the labeling of monoclonal antibody that the flushing liquor includes anti-Blood group antigen A is crossed,
If blood sample to be detected is A types, the monoclonal of the A types antigen and the anti-Blood group antigen A on nanogold particle of blood sample to be detected
Antibody is specifically bound, so as to which red blood cell fixes colour developing, if not A types, then do not develop the color.Other blood groups are similar.Thus originally
Embodiment can detect A, B, Rh blood group simultaneously, and naked eyes are visible.And the setting of panel 3 avoids the pollution of impurity, makes detection
As a result it is sensitiveer, accurate.
Certainly, the type and priority addition sequence of the addition monoclonal antibody of above-mentioned test strips 2.1,2.2 and 2.3
With change.
The base fluid of the flushing liquor is citric acid-sodium hydroxide-hydrochloride buffer, and pH value 4.7, dosage is 20 μ l.
Above-mentioned bottom plate 1 and panel 3 are polycarbonate plastic.
Fig. 3 a-3h are the testing result schematic diagram that the blood group of the utility model embodiment 2 diagnoses test card.
Embodiment 3
The present embodiment 3 is similar to Example 1, and difference is:Adsorptive pads are also bonded with the bottom plate 1, positioned at layer
The end in direction is analysed, with the lapping ends of test strips 2.1,2.2,2.3;Surplus liquid is drawn, liquid is improved and transports from the front to the back
Dynamic speed, shortens detection time.The adsorptive pads are absorbent filter.The base fluid of the flushing liquor is disodium hydrogen phosphate-citric acid
Buffer solution buffer solution, pH value 3.7, dosage are 10 μ l.
Technical scheme in above-mentioned the embodiment of the present application, at least has the following technical effect that or advantage:
The test strips being bonded on the utility model ABO/Rh blood groups diagnosis test card, including bottom plate and bottom plate, the examination
Paper slip is provided with multiple rinsing areas, and is distinguished along chromatography direction multiple loading areas corresponding with each rinsing area, the loading
Not Han You anti-A, B or Rh blood group antigens solid phase monoclonal antibody, for adding sentencing for blood sample and Blood grouping result
Read;The rinsing area is used to add flushing liquor, promotes blood sample along the flowing of chromatography direction, the flushing liquor include anti-A, B or
3 kinds of flushing liquors of the Nano-Au probe of the labeling of monoclonal antibody of Rh blood group antigens, A types, Type B or the RH positives can be marked red respectively
Cell.Blood sample to be detected is added in each loading area when the utility model detects, different rush then is separately added into each rinsing area
Washing lotion, the nanogold particle that the labeling of monoclonal antibody comprising anti-A, B or Rh blood group antigens is crossed in the flushing liquor, the flushing
Liquid moves along chromatography direction, and the erythrocyte binding of blood group corresponding to blood sample to be detected simultaneously develops the color in loading area, such as when described
When flushing liquor is the nanogold particle that the labeling of monoclonal antibody comprising anti-Blood group antigen A is crossed, if blood sample to be detected is A types,
The A types antigen of blood sample to be detected then occurs specifically with the monoclonal antibody of the anti-Blood group antigen A on nanogold particle in loading area
Property combine, so as to red blood cell fix colour developing, if not A types then not loading area develop the color, other blood groups are similar, thus this practicality newly
Type can detect A, B, Rh blood group simultaneously.
Although having been described for preferred embodiment of the present utility model, those skilled in the art once know substantially
Creative concept, then other change and modification can be made to these embodiments.So appended claims are intended to be construed to wrap
Include preferred embodiment and fall into having altered and changing for the scope of the utility model.Obviously, those skilled in the art can be with
Various changes and modification are carried out to the utility model without departing from spirit and scope of the present utility model.So, if this practicality
These new modifications and variations belong within the scope of the utility model claims and its equivalent technologies, then the utility model
It is also intended to comprising including these changes and modification.
Claims (7)
1. a kind of ABO/Rh blood groups diagnose test card, it is characterised in that:It is described including the test strips being bonded on bottom plate and bottom plate
Test strips are provided with multiple rinsing areas, and along chromatography direction multiple loading areas corresponding with each rinsing area, the loading area
Contain the solid phase monoclonal antibody of anti-A, B or Rh blood group antigens respectively, for adding blood sample and Blood grouping result
Interpretation;The rinsing area is used to add flushing liquor, promotes blood sample to include anti-A, B along the flowing of chromatography direction, the flushing liquor
Or 3 kinds of flushing liquors of the Nano-Au probe of the labeling of monoclonal antibody of Rh blood group antigens, for marking A types, Type B or RH sun respectively
Property red blood cell.
2. ABO/Rh blood groups according to claim 1 diagnose test card, it is characterised in that:The test strips are to be set up in parallel
3, its length direction is consistent with chromatography direction, in each test strips along chromatography direction be disposed with rinsing area and on
The solid phase monoclonal antibody of different blood group antigens is contained in sample area, each loading area, the loading area be used to adding blood sample with
And the interpretation of Blood grouping result, each rinsing area are used to add flushing liquor, the chromatography direction flowing of blood sample edge is promoted,
The flushing liquor includes 3 kinds of flushing liquors of the Nano-Au probe of the labeling of monoclonal antibody of anti-A, B or Rh blood group antigens, for dividing
Biao Ji not A types, Type B and RH positive red blood cells.
3. ABO/Rh blood groups according to claim 1 or 2 diagnose test card, it is characterised in that:Above the test strips also
Including a panel, the test strips are fixed on interior by the bottom plate with the space that panel is formed;Along chromatography direction on the panel
Multiple flushing holes are provided with, and are used to add along chromatography direction multiple loading holes corresponding with each flushing hole, the flushing hole
Enter the flushing liquor of the Nano-Au probe of the labeling of monoclonal antibody comprising anti-A, B or Rh blood group antigens, the loading hole is used to add
Enter blood sample to be detected.
4. ABO/Rh blood groups according to claim 3 diagnose test card, it is characterised in that:The flushing hole and loading hole point
Wei not be 3.
5. ABO/Rh blood groups according to claim 3 diagnose test card, it is characterised in that:The bottom plate and panel are hard to have
Matter paper, machine glass, polystyrene, polycarbonate-based transparent or opaque plastics.
6. ABO/Rh blood groups according to claim 1 diagnose test card, it is characterised in that:Suction is also bonded with the bottom plate
Water cushion, positioned at the end in chromatography direction, with the test strips lapping ends.
7. ABO/Rh blood groups according to claim 6 diagnose test card, it is characterised in that:The adsorptive pads are filtered for water suction
Paper.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201720960464.6U CN207081742U (en) | 2017-08-03 | 2017-08-03 | A kind of ABO/Rh blood groups diagnose test card |
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CN201720960464.6U CN207081742U (en) | 2017-08-03 | 2017-08-03 | A kind of ABO/Rh blood groups diagnose test card |
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Publication Number | Publication Date |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110398590A (en) * | 2019-06-21 | 2019-11-01 | 深圳大学 | Sensing chip and application |
CN110426524A (en) * | 2019-08-21 | 2019-11-08 | 安邦(厦门)生物科技有限公司 | A kind of double interpretation reverse type detection systems of abo blood group and detection method |
WO2021012948A1 (en) * | 2019-07-23 | 2021-01-28 | 英科新创(厦门)科技股份有限公司 | Blood group antigen testing component |
-
2017
- 2017-08-03 CN CN201720960464.6U patent/CN207081742U/en not_active Expired - Fee Related
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110398590A (en) * | 2019-06-21 | 2019-11-01 | 深圳大学 | Sensing chip and application |
CN110398590B (en) * | 2019-06-21 | 2022-11-15 | 深圳大学 | Sensing chip and application |
WO2021012948A1 (en) * | 2019-07-23 | 2021-01-28 | 英科新创(厦门)科技股份有限公司 | Blood group antigen testing component |
CN110426524A (en) * | 2019-08-21 | 2019-11-08 | 安邦(厦门)生物科技有限公司 | A kind of double interpretation reverse type detection systems of abo blood group and detection method |
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