CN111707819A - AFB1, ZEN and DON three-index quantitative chromatography test strip - Google Patents
AFB1, ZEN and DON three-index quantitative chromatography test strip Download PDFInfo
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- CN111707819A CN111707819A CN202010575359.7A CN202010575359A CN111707819A CN 111707819 A CN111707819 A CN 111707819A CN 202010575359 A CN202010575359 A CN 202010575359A CN 111707819 A CN111707819 A CN 111707819A
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- G—PHYSICS
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
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- G—PHYSICS
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
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Abstract
The invention provides an AFB, ZEN and DON three-index quantitative chromatography test strip, and relates to the technical field of reagent detection. This three index ration chromatography test paper strips of AFB, ZEN, DON, including the bottom welt, one side at bottom welt top is provided with the sample pad, the center at bottom welt top is provided with the bottom chromatographic film, the top fixedly connected with middle level chromatographic film of bottom chromatographic film, the top fixedly connected with top layer chromatographic film of middle level chromatographic film, the top of bottom welt is kept away from one side fixedly connected with and bottom chromatographic film, middle level chromatographic film and the corresponding pad that absorbs water of top layer chromatographic film of sample pad, one side that colloidal gold combined pad was kept away from to bottom chromatographic film, middle level chromatographic film and top layer chromatographic film all is provided with the quality control line. Through design bottom chromatography membrane, middle level chromatography membrane and top chromatographic column, can carry out short-term test to aflatoxin B1(AFB1), Zearalenone (ZEN) and vomitoxin (DON), convenient and fast is worth wideling popularize.
Description
Technical Field
The invention relates to the technical field of reagent detection, in particular to an AFB1, ZEN and DON three-index quantitative chromatography test strip.
Background
Aflatoxin B1 (abbreviated as AFB1) is a derivative of dihydrofuran phthalazinone, and contains a difuran ring and a phthalazinone (coumarin), aflatoxin B1 is one of the most carcinogenic known chemical substances, aflatoxin B1 has strong toxicity to human and animals, the toxic effect is mainly damage to liver, aflatoxin B1 is most common in natural foods, and the hazard is the most serious, and the national quality inspection bureau stipulates that aflatoxin B1 is one of the necessary items of most foods; zearalenone (ZEN) mainly pollutes corn, wheat, rice, barley, millet, oat and other grains, wherein the positive detection rate of the corn is 45 percent, and the highest toxic content can reach 2909 mg/kg; the detection rate of wheat is 20%, the toxicity content is 0.364-11.05 mg/kg, the heat resistance of zearalenone is strong, the zearalenone is completely destroyed after being treated for 1 hour at 110 ℃, the zearalenone has an estrogen-like effect, acute and chronic poisoning of animals can be caused, the animal reproductive function is abnormal or even dead, huge economic loss can be caused to an animal farm, and the zearalenone is a metabolite of zearalenone; vomitoxin (vomitoxi n), also known as Deoxynivalenol (DON), has the chemical name of 3 alpha, 7 alpha, 15-trihydroxy fusarium solani-9-ene-8-ketone, belongs to trichothecene compounds, is named because it can cause vomiting of pigs, has certain harm effect on human bodies, and is a third-level carcinogen according to the classification standard of European Union.
To aflatoxin B1(AFB1), Zearalenone (ZEN) and vomitoxin (DON) detection, traditional detection mode adopts different test paper strips to separately detect, adopts different test paper strips to detect not only wastes time and energy, and the operation is all very inconvenient with carrying moreover, and detection efficiency and accuracy are also lower, for this reason, we have developed a new three index quantitative chromatography test paper strips of AFB1, ZEN, DON.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides an AFB1, ZEN and DON three-index quantitative chromatography test strip, which solves the problems that different test strips are adopted to separately detect in the traditional detection mode, the detection by adopting different test strips wastes time and labor, the operation and carrying are inconvenient, and the detection efficiency and accuracy are low.
(II) technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme: the utility model provides a three index ration chromatography test paper strips of AFB, ZEN, DON, includes the bottom welt, one side at bottom welt top is provided with the sample pad, the center at bottom welt top is provided with bottom chromatography membrane, bottom chromatography membrane's top fixedly connected with middle level chromatography membrane, middle level chromatography membrane's top fixedly connected with top layer chromatography membrane, one side of sample pad is provided with the colloidal gold that corresponds with bottom chromatography membrane, middle level chromatography membrane and top layer chromatography membrane and binds the pad, the one side fixedly connected with that sample pad was kept away from at the top of bottom welt and the pad that absorbs water that bottom chromatography membrane, middle level chromatography membrane and top layer chromatography membrane correspond, one side that colloidal gold combined the pad was kept away from to bottom chromatography membrane, middle level chromatography membrane and top layer chromatography membrane all is provided with the matter control line.
Preferably, the bottom liner is made of PVC material.
Preferably, the bottom layer chromatographic membrane, the middle layer chromatographic membrane and the surface layer chromatographic membrane are fixedly connected through pressing.
Preferably, the absorbent pad is made of absorbent cotton material.
Preferably, one side of the bottom layer chromatographic membrane is provided with a first detection line corresponding to the quality control line.
Preferably, a second detection line corresponding to the quality control line is arranged at the center of the middle layer chromatography membrane.
Preferably, one side of the surface chromatographic membrane is provided with a third detection line corresponding to the quality control line.
Preferably, the plurality of quality control lines are arranged in an overlapping structure and are positioned on the same vertical straight line.
The working principle is as follows: when the AFB1, ZEN and DON three-index quantitative chromatography test strip is used, a sample mixed liquid can be dropped on a sample pad 2, the bottom layer chromatography membrane 3, the middle layer chromatography membrane 4 and the surface layer chromatography membrane 5 are formed by pressing, so that the fixed connection effect can be achieved, and meanwhile, the whole volume of the bottom layer chromatography membrane 3, the middle layer chromatography membrane 4 and the surface layer chromatography membrane 5 can be reduced, a water absorption pad 7 corresponding to the bottom layer chromatography membrane 3, the middle layer chromatography membrane 4 and the surface layer chromatography membrane 5 is fixedly connected to one side, far away from the sample pad 2, of the top of a bottom lining plate 1, the water absorption pad 7 made of a water absorption cotton material has a better water absorption effect, so that a mixed liquid dropped on the sample pad 2 can be uniformly absorbed in the bottom layer chromatography membrane 3, the middle layer chromatography membrane 4 and the surface layer chromatography membrane 5 under the water absorption effect of the water absorption pad 7, one side of the bottom layer chromatography membrane 3 is provided with a first detection line 8 corresponding to a quality control line, the bottom layer chromatographic membrane 3 and the first detection line 8 are used for detecting aflatoxin B1(AFB1), when the mixed solution contains aflatoxin B1, the first detection line 8 is red, so that the effect of quickly detecting aflatoxin B1(AFB1) is realized, the center of the middle layer chromatographic membrane 4 is provided with a second detection line 9 corresponding to a quality control line 11, the middle layer chromatographic membrane 4 and the second detection line 9 are used for detecting Zearalenone (ZEN), when the mixed solution contains Zearalenone (ZEN), the second detection line 9 is red, so that the effect of quickly detecting Zearalenone (ZEN) is realized, one side of the surface layer chromatographic membrane 5 is provided with a third detection line 10 corresponding to the quality control line 11, the surface layer chromatographic membrane 5 and the third detection line 10 are used for detecting vomitoxin (DON), when the mixed solution contains vomitoxin (DON), the third detection line 10 is red, thereby realize vomitus toxin (DON) short-term test's effect, it can wait to detect mixed liquid and turn red to be the many quality control lines 11 that the overlap structure set up adsorbing, and the mixed liquid that contains aflatoxin B1(AFB1), Zearalenone (ZEN) or vomitoxin (DON) is no matter being adsorbed to be located many quality control lines 11 on same vertical straight line, all can turn red to guarantee the validity that detects.
(III) advantageous effects
The invention provides an AFB1, ZEN and DON three-index quantitative chromatography test strip. The method has the following beneficial effects:
1. this three index quantitative chromatography test paper strips of AFB1, ZEN, DON through simple combination formula sample short-term test structure, can utilize same kind of test paper strip to accomplish the short-term test of different toxins, both convenient operation, portable has also improved detection efficiency simultaneously.
2. This three index ration chromatography test paper strips of AFB1, ZEN, DON through design bottom chromatographic film, middle level chromatographic film and top layer chromatographic film, can carry out short-term test, convenient and fast to aflatoxin B1(AFB1), Zearalenone (ZEN) and vomit toxin (DON), thereby make its work efficiency improve greatly, be worth wideling popularize.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a top view structural view of a chromatographic membrane of the present invention;
FIG. 3 is a front view structural view of a chromatographic carrier of the present invention.
Wherein, 1, a bottom lining plate; 2. a sample pad; 3. a bottom layer chromatographic membrane; 4. a middle layer chromatographic membrane; 5. a surface layer chromatographic membrane; 6. a colloidal gold bonding pad; 7. a water absorbent pad; 8. a first detection line; 9. a second detection line; 10. a third detection line; 11. and (4) quality control line.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example (b):
as shown in fig. 1-3, an embodiment of the present invention provides an AFB1, ZEN, DON three-index quantitative chromatography test strip, which comprises a bottom lining plate 1, a sample pad 2 is disposed on one side of the top of the bottom lining plate 1, a bottom layer chromatography membrane 3 is disposed in the center of the top of the bottom lining plate 1, a middle layer chromatography membrane 4 is fixedly connected to the top of the bottom layer chromatography membrane 3, a surface layer chromatography membrane 5 is fixedly connected to the top of the middle layer chromatography membrane 4, one side of the sample pad 2 is disposed with the bottom layer chromatography membrane 3, the colloidal gold combined pad 6 corresponding to the middle layer chromatographic membrane 4 and the surface layer chromatographic membrane 5, the water absorption pad 7 corresponding to the bottom layer chromatographic membrane 3, the middle layer chromatographic membrane 4 and the surface layer chromatographic membrane 5 is fixedly connected to one side of the top of the bottom lining plate 1 far away from the sample pad 2, and the quality control lines 11 are arranged on one sides of the bottom layer chromatographic membrane 3, the middle layer chromatographic membrane 4 and the surface layer chromatographic membrane 5 far away from the colloidal gold combined pad 6.
The bottom lining plate 1 is made of PVC materials, and the bottom lining plate 1 made of the PVC materials not only can play a role in supporting and fixing, but also can make the test strip structure more firm and more tensile.
The bottom chromatographic membrane 3, the middle chromatographic membrane 4 and the surface chromatographic membrane 5 are fixedly connected through pressing, and the bottom chromatographic membrane 3, the middle chromatographic membrane 4 and the surface chromatographic membrane 5 are formed through pressing, so that the fixed connection effect can be achieved, and the whole volume of the bottom chromatographic membrane 3, the middle chromatographic membrane 4 and the surface chromatographic membrane 5 can be reduced.
The absorbent pad 7 is made of absorbent cotton material, and the absorbent pad 7 made of absorbent cotton material has better water absorption effect, so that the mixed liquid dropped on the sample pad 2 can be uniformly absorbed in the bottom layer chromatographic membrane 3, the middle layer chromatographic membrane 4 and the surface layer chromatographic membrane 5 under the water absorption effect of the absorbent pad 7.
And a first detection line 8 corresponding to the quality control line 11 is arranged on one side of the bottom layer chromatographic membrane 3, the bottom layer chromatographic membrane 3 and the first detection line 8 are used for detecting aflatoxin B1(AFB1), and when the mixed liquid contains aflatoxin B1, the first detection line 8 is changed into red, so that the effect of quickly detecting aflatoxin B1(AFB1) is realized.
The center of middle level chromatographic membrane 4 is provided with the second detection line 9 corresponding with quality control line 11, and middle level chromatographic membrane 4 is used for detecting Zearalenone (ZEN) with second detection line 9, and when containing Zearalenone (ZEN) in the mixed solution, second detection line 9 can be and become red to realize Zearalenone (ZEN) short-term test's effect.
One side of top layer chromatographic membrane 5 is provided with the third detection line 10 corresponding with quality control line 11, and top layer chromatographic membrane 5 and third detection line 10 are used for detecting vomitoxin (DON), and when contain vomitoxin (DON) in the mixed liquid, third detection line 10 can be and become red to realize vomitoxin (DON) short-term test's effect.
Many quality control lines 11 all are the overlapping structure setting, and many quality control lines 11 all are located same vertical straight line, and many quality control lines 11 that are the overlapping structure setting can wait to detect to mix the liquid after red in the absorption, and many quality control lines 11 that are located same vertical straight line all can turn red after adsorbing the mixed liquid that contains aflatoxin B1(AFB1), Zearalenone (ZEN) or vomitoxin (DON), thereby guarantee the validity that detects.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. The utility model provides a three index quantitative chromatography test paper strips of AFB1, ZEN, DON, includes bottom welt (1), its characterized in that: a sample pad (2) is arranged on one side of the top of the bottom lining plate (1), a bottom layer chromatographic membrane (3) is arranged in the center of the top of the bottom lining plate (1), the top of the bottom layer chromatographic membrane (3) is fixedly connected with a middle layer chromatographic membrane (4), the top of the middle layer chromatographic membrane (4) is fixedly connected with a surface layer chromatographic membrane (5), a colloidal gold combined pad (6) corresponding to the bottom layer chromatographic membrane (3), the middle layer chromatographic membrane (4) and the surface layer chromatographic membrane (5) is arranged on one side of the sample pad (2), the side of the top of the bottom lining plate (1) far away from the sample pad (2) is fixedly connected with a water absorption pad (7) corresponding to the bottom layer chromatographic membrane (3), the middle layer chromatographic membrane (4) and the surface layer chromatographic membrane (5), and quality control lines (11) are arranged on one sides of the bottom layer chromatographic membrane (3), the middle layer chromatographic membrane (4) and the surface layer chromatographic membrane (5) far away from the colloidal gold combined pad (6).
2. The AFB1, ZEN and DON three-index quantitative chromatography test strip of claim 1, wherein: the bottom lining plate (1) is made of PVC material.
3. The AFB1, ZEN and DON three-index quantitative chromatography test strip of claim 1, wherein: the bottom layer chromatographic membrane (3), the middle layer chromatographic membrane (4) and the surface layer chromatographic membrane (5) are fixedly connected through pressing.
4. The AFB1, ZEN and DON three-index quantitative chromatography test strip of claim 1, wherein: the absorbent pad (7) is made of absorbent cotton material.
5. The AFB1, ZEN and DON three-index quantitative chromatography test strip of claim 1, wherein: one side of the bottom layer chromatographic membrane (3) is provided with a first detection line (8) corresponding to the quality control line (11).
6. The AFB1, ZEN and DON three-index quantitative chromatography test strip of claim 1, wherein: and a second detection line (9) corresponding to the quality control line (11) is arranged at the center of the middle layer chromatographic membrane (4).
7. The AFB1, ZEN and DON three-index quantitative chromatography test strip of claim 1, wherein: and a third detection line (10) corresponding to the quality control line (11) is arranged on one side of the surface chromatographic membrane (5).
8. The AFB1, ZEN and DON three-index quantitative chromatography test strip of claim 1, wherein: the multiple quality control lines (11) are arranged in an overlapping structure, and the multiple quality control lines (11) are all located on the same vertical straight line.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010575359.7A CN111707819B (en) | 2020-06-22 | 2020-06-22 | AFB1, ZEN and DON three-index quantitative chromatographic test strip |
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| CN202010575359.7A CN111707819B (en) | 2020-06-22 | 2020-06-22 | AFB1, ZEN and DON three-index quantitative chromatographic test strip |
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| CN111707819A true CN111707819A (en) | 2020-09-25 |
| CN111707819B CN111707819B (en) | 2023-07-21 |
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| Publication number | Publication date |
|---|---|
| CN111707819B (en) | 2023-07-21 |
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