GB2476525A - Immunoaffinity column for detection of Aflatoxins B1, B2, G1, G2, Ochratoxin A and Fumonisins B1, B2 - Google Patents
Immunoaffinity column for detection of Aflatoxins B1, B2, G1, G2, Ochratoxin A and Fumonisins B1, B2 Download PDFInfo
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- GB2476525A GB2476525A GB1013734A GB201013734A GB2476525A GB 2476525 A GB2476525 A GB 2476525A GB 1013734 A GB1013734 A GB 1013734A GB 201013734 A GB201013734 A GB 201013734A GB 2476525 A GB2476525 A GB 2476525A
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- United Kingdom
- Prior art keywords
- column
- resin
- fumonisins
- ochratoxin
- aflatoxins
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 229930195730 Aflatoxin Natural products 0.000 title claims abstract description 27
- 239000005409 aflatoxin Substances 0.000 title claims abstract description 27
- RWQKHEORZBHNRI-BMIGLBTASA-N ochratoxin A Chemical compound C([C@H](NC(=O)C1=CC(Cl)=C2C[C@H](OC(=O)C2=C1O)C)C(O)=O)C1=CC=CC=C1 RWQKHEORZBHNRI-BMIGLBTASA-N 0.000 title claims abstract description 26
- VYLQGYLYRQKMFU-UHFFFAOYSA-N Ochratoxin A Natural products CC1Cc2c(Cl)cc(CNC(Cc3ccccc3)C(=O)O)cc2C(=O)O1 VYLQGYLYRQKMFU-UHFFFAOYSA-N 0.000 title claims abstract description 25
- 239000003008 fumonisin Substances 0.000 title claims abstract description 25
- DAEYIVCTQUFNTM-UHFFFAOYSA-N ochratoxin B Natural products OC1=C2C(=O)OC(C)CC2=CC=C1C(=O)NC(C(O)=O)CC1=CC=CC=C1 DAEYIVCTQUFNTM-UHFFFAOYSA-N 0.000 title claims abstract description 25
- 238000001514 detection method Methods 0.000 title claims description 5
- 239000011347 resin Substances 0.000 claims abstract description 38
- 229920005989 resin Polymers 0.000 claims abstract description 38
- 239000003053 toxin Substances 0.000 claims abstract description 25
- 231100000765 toxin Toxicity 0.000 claims abstract description 25
- 108700012359 toxins Proteins 0.000 claims abstract description 25
- 239000012491 analyte Substances 0.000 claims abstract description 17
- 238000000034 method Methods 0.000 claims abstract description 11
- 239000007788 liquid Substances 0.000 claims abstract description 9
- XWIYFDMXXLINPU-UHFFFAOYSA-N Aflatoxin G Chemical compound O=C1OCCC2=C1C(=O)OC1=C2C(OC)=CC2=C1C1C=COC1O2 XWIYFDMXXLINPU-UHFFFAOYSA-N 0.000 claims abstract description 7
- 235000013305 food Nutrition 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 241001465754 Metazoa Species 0.000 claims description 5
- 235000008452 baby food Nutrition 0.000 claims description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims 4
- 239000012530 fluid Substances 0.000 claims 4
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims 3
- 239000002904 solvent Substances 0.000 claims 2
- 235000013334 alcoholic beverage Nutrition 0.000 claims 1
- 239000007853 buffer solution Substances 0.000 claims 1
- 208000024386 fungal infectious disease Diseases 0.000 claims 1
- 210000004907 gland Anatomy 0.000 claims 1
- 235000021577 malt beverage Nutrition 0.000 claims 1
- 239000002245 particle Substances 0.000 claims 1
- 239000012266 salt solution Substances 0.000 claims 1
- KQTIIICEAUMSDG-UHFFFAOYSA-N tricarballylic acid Chemical compound OC(=O)CC(C(O)=O)CC(O)=O KQTIIICEAUMSDG-UHFFFAOYSA-N 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 3
- 235000013339 cereals Nutrition 0.000 description 3
- 241000233866 Fungi Species 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 235000007319 Avena orientalis Nutrition 0.000 description 1
- 244000075850 Avena orientalis Species 0.000 description 1
- 240000005979 Hordeum vulgare Species 0.000 description 1
- 235000007340 Hordeum vulgare Nutrition 0.000 description 1
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 239000002115 aflatoxin B1 Substances 0.000 description 1
- OQIQSTLJSLGHID-WNWIJWBNSA-N aflatoxin B1 Chemical compound C=1([C@@H]2C=CO[C@@H]2OC=1C=C(C1=2)OC)C=2OC(=O)C2=C1CCC2=O OQIQSTLJSLGHID-WNWIJWBNSA-N 0.000 description 1
- WWSYXEZEXMQWHT-WNWIJWBNSA-N aflatoxin B2 Chemical compound C=1([C@@H]2CCO[C@@H]2OC=1C=C(C1=2)OC)C=2OC(=O)C2=C1CCC2=O WWSYXEZEXMQWHT-WNWIJWBNSA-N 0.000 description 1
- 229930020125 aflatoxin-B1 Natural products 0.000 description 1
- 235000013405 beer Nutrition 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 239000013626 chemical specie Substances 0.000 description 1
- 150000005690 diesters Chemical class 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 231100000573 exposure to toxins Toxicity 0.000 description 1
- UVBUBMSSQKOIBE-DSLOAKGESA-N fumonisin B1 Chemical compound OC(=O)C[C@@H](C(O)=O)CC(=O)O[C@H]([C@H](C)CCCC)[C@@H](OC(=O)C[C@@H](CC(O)=O)C(O)=O)C[C@@H](C)C[C@H](O)CCCC[C@@H](O)C[C@H](O)[C@H](C)N UVBUBMSSQKOIBE-DSLOAKGESA-N 0.000 description 1
- QZIADBYRQILELJ-UHFFFAOYSA-N fumonisin B1 Natural products CCCCC(C)C(OC(=O)CC(CC(=O)O)C(=O)O)C(C)(CC(C)CC(O)CCCCC(O)CC(O)C(C)N)OC(=O)CC(CC(=O)O)C(=O)O QZIADBYRQILELJ-UHFFFAOYSA-N 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 230000033444 hydroxylation Effects 0.000 description 1
- 238000005805 hydroxylation reaction Methods 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- QRXWMOHMRWLFEY-UHFFFAOYSA-N isoniazide Chemical group NNC(=O)C1=CC=NC=C1 QRXWMOHMRWLFEY-UHFFFAOYSA-N 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 229960005190 phenylalanine Drugs 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000011343 solid material Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
- B01D15/3804—Affinity chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
- G01N33/545—Synthetic resin
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56961—Plant cells or fungi
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- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Tropical Medicine & Parasitology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Virology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Sampling And Sample Adjustment (AREA)
- Peptides Or Proteins (AREA)
Abstract
A multi analyte immunoaffinity column is disclosed comprising at least one unit of resin having Aflatoxin specific affinity, wherein the column further contains about 2-3 units of resin containing antibody having specificity for Ochratoxin A and about 4-6 units of resin containing antibody having specificity for Fumonisins B1 and B2, wherein one unit of resin is the quantity of resin containing antibody that will bind 150ng of Aflatoxins B1, B2, G1, and G2, 160ng of Ochratoxin A and 600ng of Fumonisins B1 and B2 respectively. A method of analyzing a single liquid sample for these toxins using the aforementioned multi-analyte column is also claimed.
Description
Immunoaffinity column for detection of Aflatoxins Bi, B2, Gi, G2, Ochratoxin A and Fumonisins Bi, B2.
DESCRIPTION
A multi analyte immunoaffinity column is disclosed. The column may contain at least one unit of resin having aflatoxin specific affinity and, for each unit of resin having aflatoxin specific affinity, the column further contains about 2 -3 units of resin containing antibody having specificity for Ochratoxin A and about 4-6 units of resin containing antibody having specificity for Fumonisins Bi and B2. One unit of resin is the quantity of resin containing antibody that will bind l5Ong of Aflatoxins, l6Ong of Ochratoxin A and 600ng of Fumonisins Bi and 82.
Field of the invention
The invention is concerned with an affinity column used for immunological screening for environmentally occurring toxins, for example, those found in food products, and is particularly directed to multi analyte columns for detecting a plurality of toxins that may be present in a single sample.
Background of the invention
Awareness of the incidence and effect of human and animal exposure to toxins by humans and other animals via food, water and air is of critical importance to our survival. The detection of toxins such as Aflatoxins 81, B2, Gi and G2, Ochratoxin A and Fumonisins Bi and B2 has become especially important. In particular screening procedures for assessing the exposure of humans to such toxins may require the ability to quantify both the toxin and its metabolites.
Immunoaffinity columns for the detection of single analytes, for example, one of Aflatoxins 81, B2, Gi and G2, Ochratoxin A or Fumonisins Bi and B2 in a sample are well known; (see Stroka et al, Journal of AOAC International Vol 83 No.2, 2000, Entwistle et al Journal of AOAC Vol 83 No.6, 2000, Senyuva et al Journal of AOAC International Vol 91, No.3, 2008), however columns targeting higher numbers of chemical species necessarily must capture more diverse analytes. Aflatoxin B1(Cyclopenta[c]furo[3',2':4,5]furo[2,3h][1]benzo pyran-1,11-dione,2,3,6a,9a-tetrahyciro-4-methoxy-, (6aR,9aS)-) MW 312.3, Aflatoxin G 1 (1H,12 H-Furo [3',2':4,5]furo [2,3-h]pyrano[3,4-c] [1] benzopyran-1, 12-dione,3,4,7a, lOa-tetrahyd ro-5-methoxy-,(7aR,lOaS)-as well as their hydra forms, Aflatoxins 62 and G2 have similar structures, but very different to Ochratoxin A (L-phenylalanine N-[5-chloro-3,4-dihydro-8-hydroxy-3-methyl-1-oxo-1H2-benzopyran-7-yl] carbonyl-( R)-isocoumarin, CAS No. [303-47-9] has a molecular weight of 403.8) and Fumonisins 81 and B2 (Fumonisin B1 is the diester of propane-1,2,3-tricarboxylicacid and 2S-amino-12S, 16R-dimethyl-3S,5R,1OR, 14S,15R-pentahydroxyeicosane in which the C-14 and C-15 hydroxygroups are esterified with the terminal carboxy group of propane-1,2,3-tricarboxylic acid. FB2 shows a different hydroxylation patterns to FBi, but with a generally similar structure. Thus the preparation of multi analyte columns and their methods of use increase in complexity out of proportion to the number of toxins being added for analysis. Column development must allow for treatment of all target analytes according to similar methods in order that they all be analysed with a single column.
There have been several examples of simultaneous natural occurrence of Aflatoxins, Ochratoxin A and Furnonisins in various substrates. Human food and animal feed (particularly in cereal based products) can contain different multi toxin combinations from fungi infected grains. A desire still exists for competent multi analyte columns for analysing a plurality of toxins with a single column.
Summary of the invention
The present invention provides a multi analyte immunoaffinity column capable of analysing a single sample containing one or more of Aflatoxins Bi, B2, Gi, G2, Ochratoxin A and Fumonisins 81 and 82. The multianalyte columns in accord with the present invention comprises a first quantity of a first resin comprising an antibody having specificity for Aflatoxins Bi, B2, Gi and G2, a second quantity of a second resin comprising an antibody having specificity for Ochratoxin A, and a third quantity of a third resin comprising an antibody having specificity for Fumonisins Bi and B2.
It is desirable to obtain at least a 60%, preferably at least a 70% recovery from the column for each toxin in the sample. It is also desirable to have a column flow rate of approximately 2-3ml per minute, preferably so that a 25m1 sample will flow through the column in approximately 10 minutes. We have found that it is not possible to obtain satisfactory analytical results in a multi analyte column by merely combining the quantities of resin used in a single analyte column to analyse each particular analyte.
Thus we have found that a multi analyte column capable of analysing a single sample containing Aflatoxins 81, B2, Gi, G2, Ochratoxin A and Fumonisins Bi and B2 comprises for each unit of resin containing antibody having specificity for Aflatoxins about 2 -3 units of resin containing antibody having specificity for Ochratoxin A and about 4 -6 units of resin containing antibody having specificity for Fumonisins 81 and B2.
As used herein one unit of resin is defined as the quantity of resin containing antibody that will bind l5Ong of Aflatoxins, l6Ong of Ochratoxin A, 600ng of Fumonisins Bi and B2, respectively.
In a preferred embodiment, the multi analyte column of the present invention is capable of analysing a sample to detect Aflatoxins Bi, B2, Gi and G2, Ochratoxin A and Fumonisins Bi and B2 in the analysis of a single sample applied to the column.
The invention also provides a method for analysing a single sample for Aflatoxins Bi, B2, Gi, G2, Ochratoxin A, and Fumonisins Bi and B2, the method comprising providing a multi analyte column as described herein, applying liquid sample suspected of containing one or more of the specified toxins to bind any of the specified toxins to resins in the column, washing the column, eluting the resins and analysing the eluant for the presence of each of the specified toxins. The liquid sample can be a liquid suspected of containing toxins or a liquid extract of a solid material suspected of containing toxins. Specific examples of sample materials that can be analysed in accord with the columns of the present invention include fungi infected grains for example maize, wheat, barley, oats, beverages, for example beer, malt based drinks, animal feed and baby food. * S
Claims (11)
- Immunoaffinity column for detection of Aflatoxins Bi, B2, Gi, G2, Ochratoxin A and Fumonisins Bi, 62.CLAIMS1) A multi analyte column comprising at least 1 unit of resin having Aflatoxin specific affinity and, for each unit of resin having Aflatoxin specific affinity, the column further contains about 2 -3 units of resin containing antibody having specificity for Ochratoxin A and about 4-6 units of resin containing antibody having specificity for Fumonisins B1 and B2. Wherein one unit of resin is the quantity of resin containing antibody that will bind iSOngofAflatoxins Bi, B2, Gland G2, l6ongofOchratoxin A and 600ng of Fumonisins Bi and B2 respectively.
- 2) The multianalyte column of claim 1, wherein the column is structured and arranged to recover at least 60% and preferaby at least 70% of a toxin in a lOmI sample being analysed, said toxin being selected from the group consisting of Aflatoxins B1, B2, Gi, G2, Ochratoxin A, and Fumonisins Bi and B2.
- 3) The multianalyte column of claim 1, wherein the column is structured and arranged to have a column flow rate of approximately 2-3m1 /minute, and or wherein a flow rate of sample fluid through the column is 1 -2 drops per second.
- 4) The multi analyte column of claim 1, wherein the column is capable of analysing a sample to detect each of Aflatoxins Bi, B2, G1 G2, Ochratoxin A and Fumonisins Bl and B2.
- 5) The multi analyte column of claim 1, wherein the resin has a particle size of about 45-165 micron.
- 6) The multianalyte column of any one of the preceding claims wherein one resin, having a toxin specific affinity is layered into a column followed successively by layering into the column another resin having a different toxin specific affinity until all of the resin is in the column.
- 7) The multianalyte of any one of claims 1-5, wherein the resins having different toxin specific affinity are mixed and placed in a column.
- 8) A method of analysing a single liquid sample for Aflatoxins 81, B2, G1, G2, Ochratoxin A and Fumonisins B1 and B2, the method comprising: providing a multi analyte column comprising at least one unit of resin having Aflatoxin Bi, B2, Gl and G2 specific affinity and the column further contains about 2 -3 units of resin containing antibody having specificity for Ochratoxin A, about 4 -6 units of resin containing antibody having specificity for Fumonisins Bi and B2.Wherein, one unit of resin is the quantity of resin containing antibody that will bind lSOng of Aflatoxins Bi, B2, 61 and G2, l6Ong of Ochratoxin A and 600ng of Fumonisins Bi and B2 respectively; loading the column with a predetermined amount of a liquid or fluid sample suspected of containing one or more of the toxins selected from Aflatoxins B]., B2, 61, G2, Ochratoxin A, and Fumonisins Bi and B2 and; binding the toxins to the antibodies on the column; subsequently eluting each of the toxins in eluant; and analysing the eluant for the presence of each toxin.
- 9) The method according to claim 8, wherein toxins are extracted from a food using a water based or water compatible solvent.
- 10) The method according to claim 9, wherein the solvent is water:methanol, water:acetonitrile, ethanol, water:ethanol, a salt solution or a buffer solution.
- 11) The method according to any one of claims 8 -10, wherein the liquid or fluid sample comprises a liquid or fluid sample selected from the group consisting of a food extract, a grain extract, an alcoholic beverage, a food product or a component of a food product, a grain to be analysed for a fungi infection, a malt beverage or animal feed or baby food.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1013734A GB2476525A (en) | 2010-08-17 | 2010-08-17 | Immunoaffinity column for detection of Aflatoxins B1, B2, G1, G2, Ochratoxin A and Fumonisins B1, B2 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1013734A GB2476525A (en) | 2010-08-17 | 2010-08-17 | Immunoaffinity column for detection of Aflatoxins B1, B2, G1, G2, Ochratoxin A and Fumonisins B1, B2 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| GB201013734D0 GB201013734D0 (en) | 2010-09-29 |
| GB2476525A true GB2476525A (en) | 2011-06-29 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB1013734A Withdrawn GB2476525A (en) | 2010-08-17 | 2010-08-17 | Immunoaffinity column for detection of Aflatoxins B1, B2, G1, G2, Ochratoxin A and Fumonisins B1, B2 |
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| Country | Link |
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| GB (1) | GB2476525A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103792356A (en) * | 2012-11-04 | 2014-05-14 | 江苏维赛科技生物发展有限公司 | Preparation and detection method for ELISA kit detecting Fumonisins |
| CN103808938A (en) * | 2014-03-16 | 2014-05-21 | 吉林大学 | Method for detecting zearalenone |
| CN103808920A (en) * | 2012-11-06 | 2014-05-21 | 江苏维赛科技生物发展有限公司 | Moniliformin euzymelinked immunodetection kit |
| CN104391057A (en) * | 2014-11-24 | 2015-03-04 | 重庆市动物疫病预防控制中心 | Pretreatment kit for detecting ochratoxin A in pig tissue and application of pretreatment kit for detecting ochratoxin A in pig tissue |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1757349A1 (en) * | 2005-08-26 | 2007-02-28 | Vicam, L.P. | Multi-analyte affinity column |
| EP1787699A1 (en) * | 2005-11-17 | 2007-05-23 | Vicam, L.P. | Multi-analyte affinity column |
| EP1787698A1 (en) * | 2005-11-17 | 2007-05-23 | Vicam, L.P. | Multi-analyte affinity column for analyzing aflatoxin, fumonisin, ochratoxin and zearalenone |
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2010
- 2010-08-17 GB GB1013734A patent/GB2476525A/en not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1757349A1 (en) * | 2005-08-26 | 2007-02-28 | Vicam, L.P. | Multi-analyte affinity column |
| EP1787699A1 (en) * | 2005-11-17 | 2007-05-23 | Vicam, L.P. | Multi-analyte affinity column |
| EP1787698A1 (en) * | 2005-11-17 | 2007-05-23 | Vicam, L.P. | Multi-analyte affinity column for analyzing aflatoxin, fumonisin, ochratoxin and zearalenone |
Non-Patent Citations (5)
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103792356A (en) * | 2012-11-04 | 2014-05-14 | 江苏维赛科技生物发展有限公司 | Preparation and detection method for ELISA kit detecting Fumonisins |
| CN103808920A (en) * | 2012-11-06 | 2014-05-21 | 江苏维赛科技生物发展有限公司 | Moniliformin euzymelinked immunodetection kit |
| CN103808938A (en) * | 2014-03-16 | 2014-05-21 | 吉林大学 | Method for detecting zearalenone |
| CN104391057A (en) * | 2014-11-24 | 2015-03-04 | 重庆市动物疫病预防控制中心 | Pretreatment kit for detecting ochratoxin A in pig tissue and application of pretreatment kit for detecting ochratoxin A in pig tissue |
| CN104391057B (en) * | 2014-11-24 | 2016-08-24 | 重庆市动物疫病预防控制中心 | For detecting pretreating reagent box and the application thereof of ochratoxin A in porcine tissue |
Also Published As
| Publication number | Publication date |
|---|---|
| GB201013734D0 (en) | 2010-09-29 |
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