GB2476525A - Immunoaffinity column for detection of Aflatoxins B1, B2, G1, G2, Ochratoxin A and Fumonisins B1, B2 - Google Patents

Immunoaffinity column for detection of Aflatoxins B1, B2, G1, G2, Ochratoxin A and Fumonisins B1, B2 Download PDF

Info

Publication number
GB2476525A
GB2476525A GB201013734A GB201013734A GB2476525A GB 2476525 A GB2476525 A GB 2476525A GB 201013734 A GB201013734 A GB 201013734A GB 201013734 A GB201013734 A GB 201013734A GB 2476525 A GB2476525 A GB 2476525A
Authority
GB
United Kingdom
Prior art keywords
column
resin
fumonisins
ochratoxin
aflatoxins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GB201013734A
Other versions
GB201013734D0 (en
Inventor
Simon James Bevis
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to GB201013734A priority Critical patent/GB2476525A/en
Publication of GB201013734D0 publication Critical patent/GB201013734D0/en
Publication of GB2476525A publication Critical patent/GB2476525A/en
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302—Apparatus specially adapted for immunological test procedures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
    • G01N33/545—Synthetic resin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56961—Plant cells or fungi

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Botany (AREA)
  • Virology (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Peptides Or Proteins (AREA)

Abstract

A multi analyte immunoaffinity column is disclosed comprising at least one unit of resin having Aflatoxin specific affinity, wherein the column further contains about 2-3 units of resin containing antibody having specificity for Ochratoxin A and about 4-6 units of resin containing antibody having specificity for Fumonisins B1 and B2, wherein one unit of resin is the quantity of resin containing antibody that will bind 150ng of Aflatoxins B1, B2, G1, and G2, 160ng of Ochratoxin A and 600ng of Fumonisins B1 and B2 respectively. A method of analyzing a single liquid sample for these toxins using the aforementioned multi-analyte column is also claimed.

Description

Immunoaffinity column for detection of Aflatoxins Bi, B2, Gi, G2, Ochratoxin A and Fumonisins Bi, B2.
DESCRIPTION
A multi analyte immunoaffinity column is disclosed. The column may contain at least one unit of resin having aflatoxin specific affinity and, for each unit of resin having aflatoxin specific affinity, the column further contains about 2 -3 units of resin containing antibody having specificity for Ochratoxin A and about 4-6 units of resin containing antibody having specificity for Fumonisins Bi and B2. One unit of resin is the quantity of resin containing antibody that will bind l5Ong of Aflatoxins, l6Ong of Ochratoxin A and 600ng of Fumonisins Bi and 82.
Field of the invention
The invention is concerned with an affinity column used for immunological screening for environmentally occurring toxins, for example, those found in food products, and is particularly directed to multi analyte columns for detecting a plurality of toxins that may be present in a single sample.
Background of the invention
Awareness of the incidence and effect of human and animal exposure to toxins by humans and other animals via food, water and air is of critical importance to our survival. The detection of toxins such as Aflatoxins 81, B2, Gi and G2, Ochratoxin A and Fumonisins Bi and B2 has become especially important. In particular screening procedures for assessing the exposure of humans to such toxins may require the ability to quantify both the toxin and its metabolites.
Immunoaffinity columns for the detection of single analytes, for example, one of Aflatoxins 81, B2, Gi and G2, Ochratoxin A or Fumonisins Bi and B2 in a sample are well known; (see Stroka et al, Journal of AOAC International Vol 83 No.2, 2000, Entwistle et al Journal of AOAC Vol 83 No.6, 2000, Senyuva et al Journal of AOAC International Vol 91, No.3, 2008), however columns targeting higher numbers of chemical species necessarily must capture more diverse analytes. Aflatoxin B1(Cyclopenta[c]furo[3',2':4,5]furo[2,3h][1]benzo pyran-1,11-dione,2,3,6a,9a-tetrahyciro-4-methoxy-, (6aR,9aS)-) MW 312.3, Aflatoxin G 1 (1H,12 H-Furo [3',2':4,5]furo [2,3-h]pyrano[3,4-c] [1] benzopyran-1, 12-dione,3,4,7a, lOa-tetrahyd ro-5-methoxy-,(7aR,lOaS)-as well as their hydra forms, Aflatoxins 62 and G2 have similar structures, but very different to Ochratoxin A (L-phenylalanine N-[5-chloro-3,4-dihydro-8-hydroxy-3-methyl-1-oxo-1H2-benzopyran-7-yl] carbonyl-( R)-isocoumarin, CAS No. [303-47-9] has a molecular weight of 403.8) and Fumonisins 81 and B2 (Fumonisin B1 is the diester of propane-1,2,3-tricarboxylicacid and 2S-amino-12S, 16R-dimethyl-3S,5R,1OR, 14S,15R-pentahydroxyeicosane in which the C-14 and C-15 hydroxygroups are esterified with the terminal carboxy group of propane-1,2,3-tricarboxylic acid. FB2 shows a different hydroxylation patterns to FBi, but with a generally similar structure. Thus the preparation of multi analyte columns and their methods of use increase in complexity out of proportion to the number of toxins being added for analysis. Column development must allow for treatment of all target analytes according to similar methods in order that they all be analysed with a single column.
There have been several examples of simultaneous natural occurrence of Aflatoxins, Ochratoxin A and Furnonisins in various substrates. Human food and animal feed (particularly in cereal based products) can contain different multi toxin combinations from fungi infected grains. A desire still exists for competent multi analyte columns for analysing a plurality of toxins with a single column.
Summary of the invention
The present invention provides a multi analyte immunoaffinity column capable of analysing a single sample containing one or more of Aflatoxins Bi, B2, Gi, G2, Ochratoxin A and Fumonisins 81 and 82. The multianalyte columns in accord with the present invention comprises a first quantity of a first resin comprising an antibody having specificity for Aflatoxins Bi, B2, Gi and G2, a second quantity of a second resin comprising an antibody having specificity for Ochratoxin A, and a third quantity of a third resin comprising an antibody having specificity for Fumonisins Bi and B2.
It is desirable to obtain at least a 60%, preferably at least a 70% recovery from the column for each toxin in the sample. It is also desirable to have a column flow rate of approximately 2-3ml per minute, preferably so that a 25m1 sample will flow through the column in approximately 10 minutes. We have found that it is not possible to obtain satisfactory analytical results in a multi analyte column by merely combining the quantities of resin used in a single analyte column to analyse each particular analyte.
Thus we have found that a multi analyte column capable of analysing a single sample containing Aflatoxins 81, B2, Gi, G2, Ochratoxin A and Fumonisins Bi and B2 comprises for each unit of resin containing antibody having specificity for Aflatoxins about 2 -3 units of resin containing antibody having specificity for Ochratoxin A and about 4 -6 units of resin containing antibody having specificity for Fumonisins 81 and B2.
As used herein one unit of resin is defined as the quantity of resin containing antibody that will bind l5Ong of Aflatoxins, l6Ong of Ochratoxin A, 600ng of Fumonisins Bi and B2, respectively.
In a preferred embodiment, the multi analyte column of the present invention is capable of analysing a sample to detect Aflatoxins Bi, B2, Gi and G2, Ochratoxin A and Fumonisins Bi and B2 in the analysis of a single sample applied to the column.
The invention also provides a method for analysing a single sample for Aflatoxins Bi, B2, Gi, G2, Ochratoxin A, and Fumonisins Bi and B2, the method comprising providing a multi analyte column as described herein, applying liquid sample suspected of containing one or more of the specified toxins to bind any of the specified toxins to resins in the column, washing the column, eluting the resins and analysing the eluant for the presence of each of the specified toxins. The liquid sample can be a liquid suspected of containing toxins or a liquid extract of a solid material suspected of containing toxins. Specific examples of sample materials that can be analysed in accord with the columns of the present invention include fungi infected grains for example maize, wheat, barley, oats, beverages, for example beer, malt based drinks, animal feed and baby food. * S

Claims (11)

  1. Immunoaffinity column for detection of Aflatoxins Bi, B2, Gi, G2, Ochratoxin A and Fumonisins Bi, 62.CLAIMS1) A multi analyte column comprising at least 1 unit of resin having Aflatoxin specific affinity and, for each unit of resin having Aflatoxin specific affinity, the column further contains about 2 -3 units of resin containing antibody having specificity for Ochratoxin A and about 4-6 units of resin containing antibody having specificity for Fumonisins B1 and B2. Wherein one unit of resin is the quantity of resin containing antibody that will bind iSOngofAflatoxins Bi, B2, Gland G2, l6ongofOchratoxin A and 600ng of Fumonisins Bi and B2 respectively.
  2. 2) The multianalyte column of claim 1, wherein the column is structured and arranged to recover at least 60% and preferaby at least 70% of a toxin in a lOmI sample being analysed, said toxin being selected from the group consisting of Aflatoxins B1, B2, Gi, G2, Ochratoxin A, and Fumonisins Bi and B2.
  3. 3) The multianalyte column of claim 1, wherein the column is structured and arranged to have a column flow rate of approximately 2-3m1 /minute, and or wherein a flow rate of sample fluid through the column is 1 -2 drops per second.
  4. 4) The multi analyte column of claim 1, wherein the column is capable of analysing a sample to detect each of Aflatoxins Bi, B2, G1 G2, Ochratoxin A and Fumonisins Bl and B2.
  5. 5) The multi analyte column of claim 1, wherein the resin has a particle size of about 45-165 micron.
  6. 6) The multianalyte column of any one of the preceding claims wherein one resin, having a toxin specific affinity is layered into a column followed successively by layering into the column another resin having a different toxin specific affinity until all of the resin is in the column.
  7. 7) The multianalyte of any one of claims 1-5, wherein the resins having different toxin specific affinity are mixed and placed in a column.
  8. 8) A method of analysing a single liquid sample for Aflatoxins 81, B2, G1, G2, Ochratoxin A and Fumonisins B1 and B2, the method comprising: providing a multi analyte column comprising at least one unit of resin having Aflatoxin Bi, B2, Gl and G2 specific affinity and the column further contains about 2 -3 units of resin containing antibody having specificity for Ochratoxin A, about 4 -6 units of resin containing antibody having specificity for Fumonisins Bi and B2.Wherein, one unit of resin is the quantity of resin containing antibody that will bind lSOng of Aflatoxins Bi, B2, 61 and G2, l6Ong of Ochratoxin A and 600ng of Fumonisins Bi and B2 respectively; loading the column with a predetermined amount of a liquid or fluid sample suspected of containing one or more of the toxins selected from Aflatoxins B]., B2, 61, G2, Ochratoxin A, and Fumonisins Bi and B2 and; binding the toxins to the antibodies on the column; subsequently eluting each of the toxins in eluant; and analysing the eluant for the presence of each toxin.
  9. 9) The method according to claim 8, wherein toxins are extracted from a food using a water based or water compatible solvent.
  10. 10) The method according to claim 9, wherein the solvent is water:methanol, water:acetonitrile, ethanol, water:ethanol, a salt solution or a buffer solution.
  11. 11) The method according to any one of claims 8 -10, wherein the liquid or fluid sample comprises a liquid or fluid sample selected from the group consisting of a food extract, a grain extract, an alcoholic beverage, a food product or a component of a food product, a grain to be analysed for a fungi infection, a malt beverage or animal feed or baby food.
GB201013734A 2010-08-17 2010-08-17 Immunoaffinity column for detection of Aflatoxins B1, B2, G1, G2, Ochratoxin A and Fumonisins B1, B2 Withdrawn GB2476525A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB201013734A GB2476525A (en) 2010-08-17 2010-08-17 Immunoaffinity column for detection of Aflatoxins B1, B2, G1, G2, Ochratoxin A and Fumonisins B1, B2

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB201013734A GB2476525A (en) 2010-08-17 2010-08-17 Immunoaffinity column for detection of Aflatoxins B1, B2, G1, G2, Ochratoxin A and Fumonisins B1, B2

Publications (2)

Publication Number Publication Date
GB201013734D0 GB201013734D0 (en) 2010-09-29
GB2476525A true GB2476525A (en) 2011-06-29

Family

ID=42938039

Family Applications (1)

Application Number Title Priority Date Filing Date
GB201013734A Withdrawn GB2476525A (en) 2010-08-17 2010-08-17 Immunoaffinity column for detection of Aflatoxins B1, B2, G1, G2, Ochratoxin A and Fumonisins B1, B2

Country Status (1)

Country Link
GB (1) GB2476525A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103792356A (en) * 2012-11-04 2014-05-14 江苏维赛科技生物发展有限公司 Preparation and detection method for ELISA kit detecting Fumonisins
CN103808920A (en) * 2012-11-06 2014-05-21 江苏维赛科技生物发展有限公司 Moniliformin euzymelinked immunodetection kit
CN103808938A (en) * 2014-03-16 2014-05-21 吉林大学 Method for detecting zearalenone
CN104391057A (en) * 2014-11-24 2015-03-04 重庆市动物疫病预防控制中心 Pretreatment kit for detecting ochratoxin A in pig tissue and application of pretreatment kit for detecting ochratoxin A in pig tissue

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1757349A1 (en) * 2005-08-26 2007-02-28 Vicam, L.P. Multi-analyte affinity column
EP1787698A1 (en) * 2005-11-17 2007-05-23 Vicam, L.P. Multi-analyte affinity column for analyzing aflatoxin, fumonisin, ochratoxin and zearalenone
EP1787699A1 (en) * 2005-11-17 2007-05-23 Vicam, L.P. Multi-analyte affinity column

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1757349A1 (en) * 2005-08-26 2007-02-28 Vicam, L.P. Multi-analyte affinity column
EP1787698A1 (en) * 2005-11-17 2007-05-23 Vicam, L.P. Multi-analyte affinity column for analyzing aflatoxin, fumonisin, ochratoxin and zearalenone
EP1787699A1 (en) * 2005-11-17 2007-05-23 Vicam, L.P. Multi-analyte affinity column

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
Food Contaminants: Mycotoxins and Food Allergens, Book Chapter, ACS symposium series, Rudrabhatla Mahalakshmi, et al., 2008, "Detection of Relevant Mycotoxins in Wheat Beer and Sake by LC-MS/MS Using Prototype Immunoaffinity Column Clean-Up: A Preliminary Study" pages 241-251. *
J AOAC International, Vol. 83, No. 2, 2000, J Stroka, et al., "Immunoaffinity Column Cleanup with Liquid Chromatography Using Post-Column Bromination for Determination of Aflatoxins in Peanut Butter, Pistachio Paste, Fig Paste, and Paprika Powder: Collaborative Study", pages 320-40. *
J AOAC International, Vol. 83, No. 6, AC Entwisle, et al., "Liquid Chromatographic method with immunoaffinity column cleanup for determination of ochratoxin A in barley: collaborative study", pages 1377-83. *
J AOAC International, Vol. 91, No. 3, 2008, HZ Senyuva, et al., "Determination of fumonisins B1 and B2 in corn by liquid chromatography/mass spectrometry with immunoaffinity column cleanup: single-laboratory method validation.", pages 598-606. *
Rapid Communications in Mass Spectrometry, Vol. 21, No. 20, 2007, V Lattanzio et al., "Simultaneous determination of aflatoxins, ochratoxin A and Fusarium toxins in maize by liquid chromatography/tandem mass spectrometry after multitoxin immunoaffinity cleanup.", pages 3253-3261. *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103792356A (en) * 2012-11-04 2014-05-14 江苏维赛科技生物发展有限公司 Preparation and detection method for ELISA kit detecting Fumonisins
CN103808920A (en) * 2012-11-06 2014-05-21 江苏维赛科技生物发展有限公司 Moniliformin euzymelinked immunodetection kit
CN103808938A (en) * 2014-03-16 2014-05-21 吉林大学 Method for detecting zearalenone
CN104391057A (en) * 2014-11-24 2015-03-04 重庆市动物疫病预防控制中心 Pretreatment kit for detecting ochratoxin A in pig tissue and application of pretreatment kit for detecting ochratoxin A in pig tissue
CN104391057B (en) * 2014-11-24 2016-08-24 重庆市动物疫病预防控制中心 For detecting pretreating reagent box and the application thereof of ochratoxin A in porcine tissue

Also Published As

Publication number Publication date
GB201013734D0 (en) 2010-09-29

Similar Documents

Publication Publication Date Title
Berthiller et al. Developments in mycotoxin analysis: an update for 2015-2016
Rodriguez et al. Sensing food contaminants: advances in analytical methods and techniques
Berthiller et al. Developments in mycotoxin analysis: an update for 2012-2013
Shephard et al. Developments in mycotoxin analysis: an update for 2011-2012
Rahmani et al. Qualitative and quantitative analysis of mycotoxins
Zhang et al. A review of pretreatment and analytical methods of biogenic amines in food and biological samples since 2010
Turner et al. Analytical methods for determination of mycotoxins: a review
Ran et al. Determination of deoxynivalenol (DON) and its derivatives: Current status of analytical methods
Berthiller et al. Developments in mycotoxin analysis: an update for 2013-2014
Krska et al. Analysis of Fusarium toxins in feed
Hennion et al. Immuno-based sample preparation for trace analysis
Pennacchio et al. A near-infrared fluorescence assay method to detect patulin in food
Shephard et al. Developments in mycotoxin analysis: an update for 2009-2010
Nuo et al. An aptamer-based fluorescence assay for ochratoxin A
Chu Recent progress on analytical techniques for mycotoxins in feedstuffs
Kolosova et al. Fluorescence polarisation immunoassays for strobilurin fungicides kresoxim-methyl, trifloxystrobin and picoxystrobin
Atapattu et al. Recent advances in analytical methods for the determination of citrinin in food matrices
GB2476525A (en) Immunoaffinity column for detection of Aflatoxins B1, B2, G1, G2, Ochratoxin A and Fumonisins B1, B2
US20070117219A1 (en) AOZD multi-analyte affinity column
CA2629472C (en) Multi-analyte affinity column
GB2466536A (en) Immunoaffinity column for detection of deoxynivalenol, deoxynivalenol, T2 toxin and HT2 toxin
Scott Recent developments in methods of analysis for mycotoxins in foodstuffs
You et al. Environmental-friendly gold nanoparticle immunochromatographic assay for ochratoxin A based on biosynthetic mimetic mycotoxin-conjugates
Cai et al. Rapid and sensitive detection of tenuazonic acid in agricultural by-products based on gold nano-flower lateral flow
Gacem et al. Mycotoxins occurrence, toxicity and detection methods

Legal Events

Date Code Title Description
WAP Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1)