CN101949930A - Disc for fast detecting mycotoxin indexes - Google Patents
Disc for fast detecting mycotoxin indexes Download PDFInfo
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- CN101949930A CN101949930A CN2010102796794A CN201010279679A CN101949930A CN 101949930 A CN101949930 A CN 101949930A CN 2010102796794 A CN2010102796794 A CN 2010102796794A CN 201010279679 A CN201010279679 A CN 201010279679A CN 101949930 A CN101949930 A CN 101949930A
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- disk body
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Abstract
The invention relates to a disc for fast detecting a plurality of mycotoxin indexes, which is used for detecting mycotoxins in unprocessed food grains. The disc comprises a disc body formed by fixedly connecting a lower disc body and an upper disc body, wherein the lower disc body is radially provided with a plurality of colloidal gold immunodetection test strips which form an annular array; the absorbent paper end of the colloidal gold immunodetection test strip is positioned at the radial outer end of the annular array; the plurality of colloidal gold immunodetection test strips share a sample pad which is positioned in the center of the annular array; and the upper disc body is provided with a sample-feeding hole corresponding to the sample pad, and display windows corresponding to display areas of the colloidal gold immunodetection test strips. As the disc body is provided with the plurality of colloidal gold immunodetection test strips, the disc can simultaneously detect the plurality of indexes at a time to further improve the working efficiency and save the detection cost.
Description
Technical field
The present invention relates to the pick-up unit of raw grain mycotoxin, be specially a kind of many indexs of mycotoxin fast detecting dish.
Background technology
Raw grain is easy to generate various mycotoxins in links such as production, purchase, transportation, storage and sale.Mycotoxin is the secondary metabolic product of microorganisms, has extremely strong carcinogenicity and teratogenesis, can cause human and the death of most of animal acute poisoning, and the health of the mankind, domestic animal, poultry has been produced very big harm.Require the mycotoxin index of detection more in the raw grain, common have following six kinds of toxin: comprise aflatoxin B1 (AFB1), deoxynivalenol (DON), zearalenone (ZEN), ochratoxin (OTA), T-2 toxin (T-2), volt horse verticillium toxin B1(FB1).
At present, studying more is aflatoxin, and it is the general name of the similar compound of a class formation, and aflatoxin B1 wherein (AFB1) can start an inflammation of the liver and liver cancer, hepatic tissue and bile duct proliferation, mainly contain symptoms such as vomiting, diarrhoea, apocleisis and lethargic sleep, finally can cause liver renal failure.
The method of existing detection aflatoxin B1 mainly contains thin layer chromatography (TLC), high pressure lipuid chromatography (HPLC) (HPLC), radio-immunity detection method, enzyme linked immunosorbent assay (ELISA) etc.Wherein, the thin layer chromatography complex operation, workload is big when focusing on sample; Must use standard items, harmful to operating personnel's health; Poor sensitivity.Price costlinesses such as the required chromatograph of high pressure lipuid chromatography (HPLC), detecting device, and sample pre-treatments complexity, method for building up be difficulty relatively, is not suitable for detecting a large amount of samples; Need special technician during operation, should not apply.Use standard items in the radioimmunoassay detection method, radioactive contamination is arranged, the standard reagent life-span is short, must unite use with expensive instrument such as liquid scintillation counter, can not use in basic unit.The reagent palpus cryopreservation of enzyme linked immunosorbent assay; Test repeatability is bad, and recovery of standard addition is lower; It is uneconomical to detect individual samples with dedicated kit during detection, and will be equipped with microplate reader in the use, is not suitable for grassroots organization.
The colloid gold immune combination technology is a kind of solid phase labelling immunoassay that grows up on the basis of enzyme-linked immuno assay.This technology adopts the colloid gold immune test strip to detect mycotoxin.As shown in Figure 1, the colloid gold immune test strip is made of plastic bottom board 4, NC film 5, thieving paper 3, gold mark pad 2 and sample pad 1, and NC film 5 is provided with two detection lines, and one is response line T, and one is nature controlling line C.During detection, sample to be tested is dripped 1-3 with dropper drip observations after 2-6 minute on sample pad 1.If do not contain harmful toxins in the tested sample or content is lower than limit amount, the specific antibody release of colloid gold label and mobile in the gold mark pad 2 to NC film 5 districts, close with NC film 5 district's antigens and two resistive connections, article two, detection line is all shown in red, show that testing result is negative, the noxious material in the sample does not reach limit amount.As shown in Figure 2, if toxin that contains in the tested sample or content surpass limit amount, will combine with the antibody competition of colloid gold label, its corresponding detection line is not developed the color, the nature controlling line C of a redness only appears in the viewing area, shows that testing result is positive.Whether effectively nature controlling line C checks test strips itself, and colour developing is effective, and it is invalid not develop the color.The characteristics of this technology are simple, quick, single part of mensuration, do not need any instrument and equipment, but visual inspection result in a few minutes, and economize on the use of funds and reduce production costs, complied with the developing direction of fast detecting.But this technology can only index of one-time detection, once can only extract at some toxin, purifying, detection, and requires the mycotoxin index that detects more in the raw grain, and each goes duplicate detection, takes time and effort inefficiency.
Summary of the invention
The present invention is directed to the problems referred to above, provide a kind of cost low, many indexs of mycotoxin fast detecting dish that can a plurality of indexs of disposable detection.
According to technical scheme of the present invention: a kind of many indexs of mycotoxin fast detecting dish, comprise the disk body that is formed by fixedly connecting by following disk body and last disk body, be the radioactivity circular array on the described disk body down and show a plurality of colloid gold immune test strip, the thieving paper end of described colloid gold immune test strip is positioned at the radial outer end of described annular array, the shared sample pad of described a plurality of colloid gold immune test strip, described sample pad is positioned at the center of described annular array, described go up have on the disk body well corresponding with described sample pad and with the corresponding display window in the viewing area of described colloid gold immune test strip.
Described disk body be shaped as circle.
Described sample pad be shaped as circle, correspondingly described well is shaped as circle.
Described colloid gold immune test strip has six, respectively corresponding six kinds of mycotoxins.
Described display window be shaped as rectangle.
The present invention is arranged with a plurality of colloid gold immune test strip on disk body, can once detect a plurality of indexs simultaneously, has improved work efficiency, has saved the detection cost.
Description of drawings
Fig. 1 is the structural representation of colloid gold immune test strip of the prior art.
Fig. 2 is the detection interpretation synoptic diagram of the test strips among Fig. 1.
Fig. 3 is a structural representation of the present invention.
Fig. 4 removes the structural representation of going up disk body for the present invention.
Embodiment
Below in conjunction with accompanying drawing the specific embodiment of the present invention is further described.
As shown in Figure 3, Figure 4, the present invention is a kind of many indexs fast detecting dish that is used for detecting the raw grain mycotoxin, the colloid gold immune test strip improvement that is based on as shown in Figure 1 forms, the colloid gold immune test strip is made of plastic bottom board 4, NC film 5, thieving paper 3, gold mark pad 2 and sample pad 1, NC film 5 is provided with two detection lines, article one, be response line T, one is nature controlling line C.The present invention includes the disk body that is formed by fixedly connecting by following disk body 9 and last disk body 6, disk body can be made into different shape, the most frequently used circle that is shaped as.Be the radioactivity circular array on the following disk body 9 and show a plurality of colloid gold immune test strip, the quantity of test strips is decided on demand, and common has 6-8.The colloid gold immune test strip has six in the present embodiment, respectively six kinds of toxin: AFB1, DON, ZEN, OTA, T-2 and FB1 common, that require detection in the corresponding raw grain.Thieving paper 3 ends of colloid gold immune test strip are positioned at the radial outer end of annular array, the shared sample pad 1 of a plurality of colloid gold immune test strip, sample pad 1 is positioned at the center of annular array, sample pad 1 be shaped as circle.Have the well 7 corresponding with sample pad 1 on the last disk body 6, correspondingly the shape of well 7 also is circular.Also have the viewing area 10 corresponding display windows 8 with the colloid gold immune test strip on the last disk body 6, display window 8 be shaped as rectangle.
The course of work of the present invention is as follows:
1, vertically dripping sample 1-3 with plastic suction pipe drips in well 7(sample pad 1) on; 2, test result read in 2-6 minute, read after 6 minutes judge invalid; 3, the result judges: two red lines are arranged in the display window 8, show that the result is negative, the toxin that this hole is detected does not exceed standard; If a red line is arranged in the display window 8, show that the result is positive, this hole is detected toxin and is exceeded standard; If no red line in the display window 8 shows that the result is invalid.
The present invention has overcome the shortcoming that original colloid gold immune test strip can only an index of one-time detection, by the disk design, can once detect a plurality of indexs simultaneously, has improved work efficiency, has saved the detection cost.
Claims (5)
1. many indexs of mycotoxin fast detecting dish, it is characterized in that: comprise the disk body that is formed by fixedly connecting by following disk body (9) and last disk body (6), be the radioactivity circular array on the described disk body (9) down and show a plurality of colloid gold immune test strip, the thieving paper of described colloid gold immune test strip (3) end is positioned at the radial outer end of described annular array, the described shared sample pad of a plurality of colloid gold immune test strip (1), described sample pad (1) is positioned at the center of described annular array, described go up have on the disk body (6) well (7) corresponding with described sample pad (1) and with the corresponding display window in viewing area (10) (8) of described colloid gold immune test strip.
2. according to many indexs of the described mycotoxin of claim 1 fast detecting dish, it is characterized in that: described disk body be shaped as circle.
3. according to claim 1 or 2 described many indexs of mycotoxin fast detecting dishes, it is characterized in that: described sample pad (1) be shaped as circle, correspondingly described well (7) be shaped as circle.
4. according to claim 1 or 2 described many indexs of mycotoxin fast detecting dishes, it is characterized in that: described colloid gold immune test strip has six, respectively corresponding six kinds of mycotoxins.
5. according to claim 1 or 2 described many indexs of mycotoxin fast detecting dishes, it is characterized in that: described display window (8) be shaped as rectangle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102796794A CN101949930A (en) | 2010-09-13 | 2010-09-13 | Disc for fast detecting mycotoxin indexes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102796794A CN101949930A (en) | 2010-09-13 | 2010-09-13 | Disc for fast detecting mycotoxin indexes |
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| CN101949930A true CN101949930A (en) | 2011-01-19 |
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| CN2010102796794A Pending CN101949930A (en) | 2010-09-13 | 2010-09-13 | Disc for fast detecting mycotoxin indexes |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102221623A (en) * | 2011-03-30 | 2011-10-19 | 厦门信道生物技术有限公司 | Adding and push-out device suitable for plate-type reagent |
| CN106442978A (en) * | 2016-10-08 | 2017-02-22 | 北京国科华仪科技有限公司 | Y-shaped two-channel fluorescence immunochromatography test strip and preparation method thereof |
| CN107367605A (en) * | 2017-08-17 | 2017-11-21 | 武汉璟泓万方堂医药科技股份有限公司 | Collaurum/fluorescent test paper chip |
| CN107727842A (en) * | 2017-11-13 | 2018-02-23 | 山东省环境保护科学研究设计院 | A kind of preparation and application of clenbuterol hydrochloride full constituent residual joint inspection test card |
| CN109187945A (en) * | 2018-09-29 | 2019-01-11 | 厦门大学 | A kind of laying method of colloid gold test paper on CD |
| CN109283174A (en) * | 2018-09-29 | 2019-01-29 | 厦门大学 | A kind of quantitative detection CD and detection method |
| CN109596825A (en) * | 2019-02-14 | 2019-04-09 | 上海美凯纯生物科技有限公司 | Multilayer down flow type chromatography detection cartridge and preparation method thereof |
| CN110133311A (en) * | 2019-05-31 | 2019-08-16 | 河南冠宇仪器有限公司 | Mycotoxin rapid quantitative detection instrument |
| CN113406322A (en) * | 2021-06-16 | 2021-09-17 | 枣庄科技职业学院 | Convenient immunochromatography assay kit |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2833595Y (en) * | 2005-08-24 | 2006-11-01 | 万积成 | Colloid gold test paper for quick detection of metalaxyl residue |
| CN101144819A (en) * | 2007-11-09 | 2008-03-19 | 北京望尔生物技术有限公司 | Colloidal gold test paper for detecting sulphaquinoxaline drug residue |
| CN201555849U (en) * | 2009-11-18 | 2010-08-18 | 吉林大学第一医院 | Disc type colloidal gold immunochromatography combined detection plate |
| CN201903542U (en) * | 2010-09-13 | 2011-07-20 | 无锡市金坤生物工程有限公司 | Fast detection disc for multiple indexes of mycotoxins |
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2010
- 2010-09-13 CN CN2010102796794A patent/CN101949930A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2833595Y (en) * | 2005-08-24 | 2006-11-01 | 万积成 | Colloid gold test paper for quick detection of metalaxyl residue |
| CN101144819A (en) * | 2007-11-09 | 2008-03-19 | 北京望尔生物技术有限公司 | Colloidal gold test paper for detecting sulphaquinoxaline drug residue |
| CN201555849U (en) * | 2009-11-18 | 2010-08-18 | 吉林大学第一医院 | Disc type colloidal gold immunochromatography combined detection plate |
| CN201903542U (en) * | 2010-09-13 | 2011-07-20 | 无锡市金坤生物工程有限公司 | Fast detection disc for multiple indexes of mycotoxins |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102221623A (en) * | 2011-03-30 | 2011-10-19 | 厦门信道生物技术有限公司 | Adding and push-out device suitable for plate-type reagent |
| CN102221623B (en) * | 2011-03-30 | 2013-06-05 | 厦门信道生物技术有限公司 | Adding and push-out device suitable for plate-type reagent |
| CN106442978A (en) * | 2016-10-08 | 2017-02-22 | 北京国科华仪科技有限公司 | Y-shaped two-channel fluorescence immunochromatography test strip and preparation method thereof |
| CN107367605A (en) * | 2017-08-17 | 2017-11-21 | 武汉璟泓万方堂医药科技股份有限公司 | Collaurum/fluorescent test paper chip |
| CN107727842A (en) * | 2017-11-13 | 2018-02-23 | 山东省环境保护科学研究设计院 | A kind of preparation and application of clenbuterol hydrochloride full constituent residual joint inspection test card |
| CN109187945A (en) * | 2018-09-29 | 2019-01-11 | 厦门大学 | A kind of laying method of colloid gold test paper on CD |
| CN109283174A (en) * | 2018-09-29 | 2019-01-29 | 厦门大学 | A kind of quantitative detection CD and detection method |
| CN109187945B (en) * | 2018-09-29 | 2019-07-12 | 厦门大学 | A kind of laying method of colloid gold test paper on CD |
| CN109596825A (en) * | 2019-02-14 | 2019-04-09 | 上海美凯纯生物科技有限公司 | Multilayer down flow type chromatography detection cartridge and preparation method thereof |
| CN110133311A (en) * | 2019-05-31 | 2019-08-16 | 河南冠宇仪器有限公司 | Mycotoxin rapid quantitative detection instrument |
| CN113406322A (en) * | 2021-06-16 | 2021-09-17 | 枣庄科技职业学院 | Convenient immunochromatography assay kit |
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Application publication date: 20110119 |