CN109283174A - A kind of quantitative detection CD and detection method - Google Patents
A kind of quantitative detection CD and detection method Download PDFInfo
- Publication number
- CN109283174A CN109283174A CN201811147198.0A CN201811147198A CN109283174A CN 109283174 A CN109283174 A CN 109283174A CN 201811147198 A CN201811147198 A CN 201811147198A CN 109283174 A CN109283174 A CN 109283174A
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- liquid
- storage tank
- liquid storage
- test paper
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
Abstract
The invention discloses a kind of quantitative detection CD and detection methods, overflow ducts, liquid storage tank and boss channel are set on the microchannel of centrifugal CD micro-fluidic chip, overflow ducts open the feed pathway shunting of liquid storage tank, boss channel after liquid storage tank by face compared with liquid storage tank height, the boss channel other end is connected with immersion liquid pond, and test paper sample introduction end is placed in immersion liquid pond.The present invention uses detection means of the CD in conjunction with test paper, and using the directionality reacted between two phases, by the rotation of CD so that there is relative displacement between two phases, reaction can be cumulative to reinforce signal in a certain region, obtains detection data.This detection mode high degree of automation is not necessarily to manual titration, saves this complex device of a model machine, cost is reduced, while a plurality of Test paper can be integrated on CD, while carrying out multinomial identical or different detection, detecting sample can also be diversified, substantially increases detection efficiency and detection accuracy.
Description
Technical field
The present invention relates to immunity inspection field more particularly to a kind of quantitative detection CD and detection methods.
Background technique
With social development, people increasingly pay attention to health and food safety, therefore physical examination and food inspection are also therewith
Development.Heterogeneous inspection is the common technology in immunity inspection, i.e., by between two-phase any in solid, liquid, gas three-phase or three-phase
Reaction obtain required data, because the reaction between out of phase can carry out data investigation in a certain conversion zone, be obtained
Data-signal compared with homogeneous reaction when the data-signal that disperses in decades of times or hundred times of growth, therefore be widely used in detecting
Field.
Common test paper detection is one of the heterogeneous detection means between solid-liquid, mainly utilizes the capillary of test paper
Siphonage of the pipe to liquid, natural diffuseness are presented various different colours in reaction zone superposition and obtain detection data, this inspection
Survey method is very high to the time requirement of the position of detection liquid titration, the quantity of titration and reaction, and manual titration is difficult accurately,
It needs to carry out by equipment such as model machines.Therefore equipment is more, and volume is big, at high cost, inconvenient to carry.
The sample detection at many scenes often influences detection time and detection efficiency, or even because sample because volume is excessive
Holding time too long influence testing result, therefore detection device is reduced, reducing volume is one of development trend.
The present invention is that " IPS cell --- tumour cell is smart in vitro based on project of national nature science fund project " 31371444 "
The content of micro environment control chip lab new industrial research when closely knit " is unfolded.
Summary of the invention
The present invention provides a kind of quantitative detection CD and detection method, big to change existing detection device volume, not portable
The problem of band, can accurately control liquid inlet volume and feed liquor position, improve detection accuracy.
Technical problem solved by the invention can be realized using following technical scheme:
A kind of quantitative detection CD is a kind of centrifugal CD micro-fluidic chip, it is characterized in that: including CD ontology, sample
Product rest area, microchannel, immersion liquid pond, test paper placing groove, the center of the CD ontology with motor output shaft for being connected, center
Surrounding is equipped with sample deposition, and the sample deposition connects a plurality of microchannel, connects immersion liquid pond and test paper behind shown microchannel
Placing groove, the microchannel include overflow ducts, liquid storage tank and boss channel, and the overflow ducts are by-pass, and setting is being stored up
The feed pathway shunting of liquid storage tank is opened, defines the maximum liquid storage amount of liquid storage tank, liquid storage tank rear end by liquid pool feed pathway side
For boss channel, boss channel by face compared with liquid storage tank height, the other end in boss channel is connected with immersion liquid pond, and the test paper is put
It sets slot to be connected with immersion liquid pond, not communicated with microchannel, test paper is placed in the test paper placing groove, the sample introduction end of the test paper is placed in
In immersion liquid pond.
Preferably, the sample deposition is one of one of ring-shaped connection or the multiple regions separated.
Preferably, the immersion liquid pond is low compared with boss channel, and with liquid storage tank same plane, the width in the immersion liquid pond is compared with boss
The width in channel is big.
A kind of quantitative detecting method is detected, feature using a kind of quantitative detection CD described in claim 1
It is: the following steps are included:
Step 1: control CD speed, to control direction and the intensity of disc radial direction driving force；
Step 2: the change in driving force direction causes the interior liquid that can retain of the liquid storage tank of boss channel barrier to generate
Liquid level angle, and allow the liquid maximum volume that can retain that the variation that available functions calculate occurs；
Step 3: changing the volume of the liquid storage tank and the height in boss channel, controls liquid level by control revolving speed
Angle, to determine the amount of liquid in liquid storage tank and immersion liquid pond；
Step 4: controlling the revolving speed of CD, and fluid sample is allowed to remain among immersion liquid pond；
Step 5: control centrifugal force allows liquid to overcome centrifugal force to move upward to one by the siphonage of the test paper
Fixed height；And add up when reaching conversion zone and reinforce signal, testing result is obtained by the color change of conversion zone.
The present invention passes through light using the directionality reacted between two phases using detection means of the CD in conjunction with test paper
So that there is relative displacement between two phases, reaction can be cumulative to reinforce signal in a certain region, obtains testing number for the rotation of disk
According to.Liquid storage tank and by-pass are set in micro-fluidic chip formula CD simultaneously, liquid inlet volume just can control by control revolving speed, trying
Immersion liquid pond is arranged in paper liquid feeding end, guarantees sufficiently impregnate at test paper feed liquor, according to the speed control CD rotation of test paper infiltration
Time realizes accurately quantitatively positioning timing detection.
This detection mode high degree of automation is not necessarily to manual titration, saves this complex device of a model machine, reduce at
This, while a plurality of Test paper can be integrated on CD, while carrying out multinomial identical or different detection, detection sample can also be more
Sample substantially increases detection efficiency and detection accuracy.Compact of the present invention, it is easy to carry, convenient for sample detection on the spot.
Detailed description of the invention
Fig. 1 is structural schematic diagram of the invention.
Fig. 2 is liquid motion schematic diagram, wherein liquid level angle schematic diagram when Fig. 2 a is compared with the slow-speed of revolution, Fig. 2 b are to improve to turn
Liquid level angle schematic diagram when fast, liquid level angle schematic diagram when Fig. 2 c is final revolving speed.
In figure, 1- CD ontology, the sample deposition 2-, the microchannel 3-, 4- immersion liquid pond, 5- test paper placing groove, 6- test paper,
31- overflow ducts, 32- liquid storage tank, 33- boss channel, 61- sample introduction end.
Specific embodiment
The present invention is described in further detail below with reference to embodiment, embodiments of the present invention are not limited thereto.
If Fig. 1-2 is one embodiment of the present invention, including CD ontology 1, sample deposition 2, microchannel 3, immersion liquid
Pond 4, test paper placing groove 5.
CD ontology 1 is made (such as acrylic plate or polycarbonate transparent material) using transparent plastic, center be used for
Motor output shaft is fixedly connected, and sample deposition 2 is equipped with around center, and the present embodiment is suitable for fluid sample, sample deposition 2
It can be connection, such as ring-shaped, only place a kind of sample, be also possible to separate, place various samples.Sample deposition 2
A plurality of microchannel 3 is connected, as shown in Figure 1, every microchannel 3 can individually connect a sample deposition 2, is also possible to a plurality of
Microchannel 3 connects the same sample deposition 2, it can detection same sample also can detecte different samples, sample deposition
The maximum number of the microchannel 3 of 2 connections is determined by the space size of CD ontology 1.Microchannel 3 then connects immersion liquid pond 4 and examination
Paper placing groove 5.Test paper 6 is placed in test paper placing groove 5.
CD of the invention is a kind of centrifugal CD micro-fluidic chip, it is realized using centrifugal force as liquid stream driving force
The micro-fluidic system that liquid stream is tested and analyzed.In recent years, centrifugal CD micro-fluidic chip is with its high throughput, multiple parallel point
The advantages that analysing, is easy to operate, highly integrated obtains fast development.Colloid gold test paper is not yet in the micro-fluidic core of centrifugal CD at present
It is applied in piece detection, it is the key that detection that can test paper, which be able to sufficiently infiltrate in optical disc passes,.
The total flow direction of liquid is extrorse from center in microchannel on centrifugal CD micro-fluidic chip.Due to liquid
Know from experience by from the various inhibitions such as valve, gravity, surface tension, frictional force, overcomes these resistances in no driving force
When hindering effect, liquid, especially small drop can't active flow forward.Only when driving force is more than to hinder power
When, liquid just can flow forward.
Precision control of the driving force by revolving speed, accurately generates range 0- when 0-2000rpm in horizontal direction
The driving force of 223.8g be closely controlled travelling forward for liquid can.To rotate CD itself as reference frame, rotation
Turn acceleration (a) suffered by any particle on CD from the gravity (g) of the earth, rotates the centrifugal acceleration of generation
The velocity variations (ac) of (ar, equal in magnitude contrary with centripetal acceleration) and optical disk rotary.
Wherein a=g+ar+ac.(they are all vectors, and by taking CD rotates horizontally as an example, for g to the earth's core, ar is outside along radius,
Ac is then tangential direction)
When the rotation speed change of CD is little, ac be can be ignored, and g is constant, when revolving speed is in 2000rpm
The centrifugal acceleration of the 5cm radius disc periphery particle of setting value is (2Pi*2000/60) ^2* between (turnning circle per minute)
0.05=2193ms-2=223.8g.Be superimposed with the acceleration of gravity of 1g, as a result, 1/223.8 gradient, almost along
The direction of radius horizontally outward.
Therefore by control revolving speed, it can achieve the mesh of direction (level angle) and intensity of control disc radial direction driving force
's.
Microchannel 3 includes overflow ducts 31, liquid storage tank 32 and boss channel 33, and overflow ducts 31 are by-pass, and setting exists
The feed pathway shunting of liquid storage tank 32 is opened, defines the maximum liquid storage amount of liquid storage tank 32 by the feed pathway side of liquid storage tank 32,
32 rear end of liquid storage tank be boss channel 33, boss channel 33 it is high compared with liquid storage tank 32 by face, the other end in boss channel 33 with
Immersion liquid pond 4 is connected.Overflow ducts 31 can be one or more of.
The liquid that can retain in the liquid storage tank 32 that the change in driving force direction causes boss channel 33 to stop generates liquid
Face angle degree, and allow the liquid maximum volume that can retain that the variation that available functions calculate occurs.
Liquid level angle is controlled by revolving speed, it, can be with by designing the volume of by-pass position control liquid storage tank 32 in structure
Realize the accurate control of the initial volume and elution volume of liquid storage tank 32.
Liquid motion schematic diagram when Fig. 2 is optical disk rotary is the liquid motion of a microchannel 3, Fig. 2 a as shown in the figure
When for compared with the slow-speed of revolution, liquid enters liquid storage tank 32, and extra liquid flows out from the side, and quantitative liquid is retained in liquid storage tank 32,
Fig. 2 b is to improve revolving speed, liquid level angulation change, and liquid flows into the situation in immersion liquid pond 4, and Fig. 2 c is final revolving speed, and liquid level reaches final
Angle, the amount in liquid storage tank 32 are determined that the amount flowed into immersion liquid pond 4 is also determined by liquid level angle.
When the liquid inlet volume of different microchannels 3 requires different, the volume of liquid storage tank 32 and the height in boss channel 33 can be changed
Degree is controlled liquid level angle by control revolving speed and realizes the amount for determining liquid in liquid storage tank 32 and immersion liquid pond 4.
Immersion liquid pond 4 is low compared with boss channel 33, can be with 32 same plane of liquid storage tank, and the width in immersion liquid pond 4 is compared with boss channel 33
Width it is big, after optical disk rotary, the liquid in immersion liquid pond 4 will not be flowed back in boss channel 33, also with driving force generate liquid
Face angle degree, simultaneously because width increases, liquid storage volume is big, and CD upper surface is higher than boss channel 33, and liquid does not overflow immersion liquid
Pond 4.
Test paper placing groove 5 is connected with immersion liquid pond 4, does not communicate with microchannel 3, and test paper 6 is placed in test paper placing groove 5, into
Sample end 61 is then placed in immersion liquid pond 4.After optical disk rotary, the liquid level angle generated in immersion liquid pond 4 can allow the immersion liquid at sample introduction end 61
Face increases, more conducively siphon.
When CD 1 rotates, with the stress of the liquid in the microchannel 3 and microchannel 3 rotated together on CD 1
Direction is close to radially outer, and fluid sample enters along immersion liquid pond 4 along microchannel 3.When CD 1 accelerates rotation to increase centrifugal force
When, fluid sample is built up in immersion liquid pond 4.The inspection in immersion liquid pond 4 is protruded into from eminence (for centrifugal force)
Test paper 6 can be infiltrated because of the liquid occurred in immersion liquid pond 4, and the siphonage of test paper 6 enables fluid sample along examination
Paper 6 moves upwards, and the revolving speed of control CD 1 can also control centrifugal force, when centrifugal force reaches a certain size, so that it may allow
Fluid sample remains among immersion liquid pond 4.Control centrifugal force is unlikely to too big, so that it may which liquid can be made by siphon
With overcoming centrifugal force to move upward to certain height.Liquid adds up when reaching conversion zone reinforces signal, passes through reaction zone
The color change in domain obtains testing result.Testing result can with the naked eye direct-reading.
The above is only present pre-ferred embodiments, is not intended to limit the scope of the present invention, therefore
Any subtle modifications, equivalent variations and modifications to the above embodiments according to the technical essence of the invention, still fall within
Protection scope of the present invention.
Claims (4)
1. a kind of quantitative detection CD is a kind of centrifugal CD micro-fluidic chip, it is characterized in that: including CD ontology, sample
Rest area, microchannel, immersion liquid pond, test paper placing groove, the center of the CD ontology with motor output shaft for being connected, center week
It encloses and is equipped with sample deposition, the sample deposition connects a plurality of microchannel, connects immersion liquid pond behind shown microchannel and test paper is put
Slot is set, the microchannel includes overflow ducts, liquid storage tank and boss channel, and the overflow ducts are by-pass, is arranged in liquid storage
The feed pathway shunting of liquid storage tank is opened, defines the maximum liquid storage amount of liquid storage tank, liquid storage tank rear end is by pond feed pathway side
Boss channel, boss channel by face compared with liquid storage tank height, the other end in boss channel is connected with immersion liquid pond, the test paper placement
Slot is connected with immersion liquid pond, does not communicate with microchannel, test paper is placed in the test paper placing groove, the sample introduction end of the test paper is placed in leaching
In liquid pool.
2. a kind of quantitative detection CD according to claim 1, it is characterized in that: the sample deposition is ring-shaped connection
One or one of multiple regions separated.
3. a kind of quantitative detection CD according to claim 1, it is characterized in that: the immersion liquid pond is low compared with boss channel, with
The width of liquid storage tank same plane, the immersion liquid pond is big compared with the width in boss channel.
4. a kind of quantitative detecting method, detected using a kind of quantitative detection CD described in claim 1, it is characterized in that:
The following steps are included:
Step 1: control CD speed, to control direction and the intensity of disc radial direction driving force；
Step 2: the change in driving force direction causes the interior liquid that can retain of the liquid storage tank of boss channel barrier to generate liquid level
Angle, and allow the liquid maximum volume that can retain that the variation that available functions calculate occurs；
Step 3: changing the volume of the liquid storage tank and the height in boss channel, controls liquid level angle by control revolving speed,
To determine the amount of liquid in liquid storage tank and immersion liquid pond；
Step 4: controlling the revolving speed of CD, and fluid sample is allowed to remain among immersion liquid pond；
Step 5: control centrifugal force allows liquid to overcome centrifugal force to move upward to by the siphonage of the test paper certain
Highly；And add up when reaching conversion zone and reinforce signal, testing result is obtained by the color change of conversion zone.
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| CN201811147198.0A CN109283174A (en) | 2018-09-29 | 2018-09-29 | A kind of quantitative detection CD and detection method |
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| CN201811147198.0A CN109283174A (en) | 2018-09-29 | 2018-09-29 | A kind of quantitative detection CD and detection method |
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Cited By (5)
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| CN109884328A (en) * | 2019-03-01 | 2019-06-14 | 清华大学 | Lateral flow immune detection system based on centrifugal type microfludic system |
| CN110849868A (en) * | 2019-10-21 | 2020-02-28 | 江苏大学 | Intelligent detection device and method for potassium osmidate in flour based on micro-fluidic chip |
| CN112033953A (en) * | 2019-06-03 | 2020-12-04 | 利多(香港)有限公司 | Micro-fluidic chip and application |
| CN113325170A (en) * | 2021-06-16 | 2021-08-31 | 博奥生物集团有限公司 | Test paper chip device, micro-fluidic chip and lateral flow test paper strip |
| CN112033953B (en) * | 2019-06-03 | 2024-04-19 | 利多(香港)有限公司 | Microfluidic chip and application |
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