CN109884328A - Lateral flow immune detection system based on centrifugal type microfludic system - Google Patents
Lateral flow immune detection system based on centrifugal type microfludic system Download PDFInfo
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- CN109884328A CN109884328A CN201910156152.3A CN201910156152A CN109884328A CN 109884328 A CN109884328 A CN 109884328A CN 201910156152 A CN201910156152 A CN 201910156152A CN 109884328 A CN109884328 A CN 109884328A
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Abstract
The invention discloses a kind of lateral flow immune detection systems based on centrifugal type microfludic system, wherein, centrifugal type microfludic system includes centrifugal type microfludic chip, it include: lateral flow immune response film, for the centrifugal type microfludic chip upstream reaction cavity and downstream reaction cavity of connection, and liquid is adjusted by regulation centrifugal rotational speed and flows through the flow velocity that lateral flow is immunoreacted film；Lateral flow immune detection chip, lateral flow immune detection chip are made of sample-adding chamber, reaction chamber, lateral flow reaction film pipeline, waste liquid pool and microchannel, for carrying out default sample process to target sample and detecting the treatment process of target sample；Centrifugal control device, for carrying out rotational speed regulation, positioning and imaging to lateral flow immune detection chip.Lateral flow immunoassay technology is able to achieve sample volume, the reaction adjustable controllable lateral flow immune detection of flow velocity by the lateral flow immune detection system together with centrifugal type microfludic Integration ofTechnology, realizes higher detection effect.
Description
Technical field
The present invention relates to microfluidic art, in particular to a kind of lateral flow based on centrifugal type microfludic system is immune
Detection system.
Background technique
Lateral flow immunoassay technology is a kind of diagnostic techniques at low cost, reaction is fast, easy to operate, health care,
It is widely used in the fields such as food safety, environmental monitoring.
Traditional lateral flow testing product is based on lateral flow test strip.However, the application of test strips is limited to it
Detection sensitivity.On the one hand, the structure of test strips itself limits loading sample volume；Another aspect test strips passively liquid
Driving method also results in the uncontrollable of reaction process reaction flow velocity.It is sensitive that the two limits its detection to a certain extent
Degree.
Summary of the invention
The application is to be made based on inventor to the understanding of following problems and discovery:
Centrifugal type microfludic technology is that each reaction member is integrated in one piece of rotatable chip by structures such as microchannels
On, to be centrifuged power drive liquid, the complex operations and reaction process of liquid are completed on a lesser chip, realize various lifes
A kind of technology of object and medicine detection.Due to its with integrated, automation and reagent consumption the small, advantages such as low in cost,
Centrifugal type microfludic technology all obtains extensive concern in scientific research field and industry.
The present invention is directed to solve at least some of the technical problems in related technologies.
For this purpose, it is an object of the invention to propose a kind of lateral flow immune detection system based on centrifugal type microfludic system
System is able to achieve sample volume, the reaction adjustable controllable lateral flow immune detection of flow velocity, realizes higher detection effect.
In order to achieve the above objectives, it is immune to propose a kind of lateral flow based on centrifugal type microfludic system for the embodiment of the present invention
Detection system, the centrifugal type microfludic system includes centrifugal type microfludic chip, wherein the lateral flow immune detection system
It include: lateral flow immune response film, for being connected to the centrifugal type microfludic chip upstream reaction cavity and downstream reaction chamber
Body, and liquid is adjusted by regulation centrifugal rotational speed and flows through the flow velocity that the lateral flow is immunoreacted film；Lateral flow immune detection core
Piece, the lateral flow immune detection chip is by sample-adding chamber, reaction chamber, lateral flow reaction film pipeline, waste liquid pool and microchannel
Composition, for carrying out default sample process to target sample and detecting the treatment process of the target sample；Centrifugal manipulation dress
It sets, for carrying out rotational speed regulation, positioning and imaging to the lateral flow immune detection chip.
The lateral flow immune detection system based on centrifugal type microfludic system of the embodiment of the present invention is based on centrifugal miniflow
Platform is controlled, the visualization lateral flow immune detection system that a kind of sample volume is adjustable, flow velocity is controllable is provided, the sensitive of detection is improved
Degree, by lateral flow immunoassay technology together with centrifugal type microfludic Integration ofTechnology, to be able to achieve sample volume, reaction solution
Controllable lateral flow immune detection that flow velocity is adjustable, realizes higher detection effect.
In addition, the lateral flow immune detection system according to the above embodiment of the present invention based on centrifugal type microfludic system is also
It can have following additional technical characteristic:
Further, in one embodiment of the invention, the lateral flow immune response film is arcuate structure.
Further, in one embodiment of the invention, centrifugal control device is by imaging device, imaging device support
Disk, imaging chamber, LED (Light Emitting Diode, light emitting diode) planar light source, chip tray, photoelectric door, motor,
Pedestal is constituted.
Further, in one embodiment of the invention, it is previously stored in the lateral flow immune detection chip solid
State reagent, wherein the solid state reagents enter lateral flow immune response film for reacting after mixing with the target sample.
Further, in one embodiment of the invention, the target sample includes plasma sample, whole blood sample, food
Object is homogenized sample and finger tip blood sample.
Further, in one embodiment of the invention, the lateral flow immune detection chip further includes the first function
Module, wherein first functional module by the first sample addition zone, the first reaction chamber, the first reaction film cavity, the first waste liquid chamber,
First microchannel, sample holes and the first venthole composition, for carrying out default sample process to the plasma sample and detecting institute
State the treatment process of plasma sample.
Further, in one embodiment of the invention, the default sample process is the plasma sample first
Reaction was completed after preset rotation speed and the first preset time or the plasma sample is after the second preset rotation speed and the second preset time
Reaction was completed, wherein first preset rotation speed is greater than second preset rotation speed, and first preset time is pre- less than second
If the time.
Further, in one embodiment of the invention, the lateral flow immune detection chip further includes the second function
Module, wherein second functional module by the second sample addition zone, the separation of whole blood haemocyte and quantitative cavity, buffer Sample application room,
Buffer transfer chamber, the first reagent cryodesiccation chamber, the second reaction chamber, the second reaction film cavity, second the second microchannel of waste liquid chamber and
Second venthole composition is processed for carrying out default sample process to the whole blood sample and detecting the whole blood sample
Journey.
Further, in one embodiment of the invention, the lateral flow immune detection chip further includes third function
Module, wherein the third functional module is separated by solid-liquid separation by third sample addition zone, food homogenate freezes with quantitative cavity, the second reagent
Dry chamber, third reaction chamber, third reaction film cavity, third waste liquid chamber, third microchannel and third venthole composition, for institute
Food homogenate sample is stated to carry out default sample process and detect the treatment process of the food homogenate sample.
Further, in one embodiment of the invention, the lateral flow immune detection chip further includes the 4th function
Module, wherein the 4th functional module is by sample suction pipeline, the separation of whole blood haemocyte and quantifies cavity, buffer chamber, mixing chamber, divides
Distribution pipes, third reagent cryodesiccation chamber, the 4th reaction chamber, the 4th reaction film cavity, the 4th waste liquid chamber, the 4th microchannel composition, are used
In the treatment process for carrying out default sample process and the detection finger tip blood sample to the finger tip blood sample.
The additional aspect of the present invention and advantage will be set forth in part in the description, and will partially become from the following description
Obviously, or practice through the invention is recognized.
Detailed description of the invention
Above-mentioned and/or additional aspect and advantage of the invention will become from the following description of the accompanying drawings of embodiments
Obviously and it is readily appreciated that, in which:
Fig. 1 is the lateral flow immune detection system based on centrifugal type microfludic system according to one embodiment of the invention
Structural schematic diagram；
Fig. 2 is the centrifugal control device structural schematic diagram according to one embodiment of the invention；
Fig. 3 is the reaction membrane structure diagram according to one embodiment of the invention；
Fig. 4 is according to the chip structure schematic diagram in the embodiment one, two of one embodiment of the invention；
Fig. 5 a shows reaction solution volume in test strips or flows through and change over time curve on centrifugal lateral flow chip
Schematic diagram；
Fig. 5 b shows test strips and centrifugal lateral flow chip after the same reaction liquid of different volumes sufficiently reacts
Signal strength contrast schematic diagram；
Fig. 6 a shows handling schematic diagram of the centrifugal lateral flow chip with rotation speed change to reaction flow velocity；
Fig. 6 b show same reaction liquid product under, signal obtained under centrifugal lateral flow chip different rotating speeds
Strength Changes schematic diagram；
Fig. 7 is according to the chip structure schematic diagram in the embodiment three of one embodiment of the invention；
Fig. 8 is according to the chip structure schematic diagram in the example IV of one embodiment of the invention；
Fig. 9 is according to the chip structure schematic diagram in the embodiment five of one embodiment of the invention.
Specific embodiment
The embodiment of the present invention is described below in detail, examples of the embodiments are shown in the accompanying drawings, wherein from beginning to end
Same or similar label indicates same or similar element or element with the same or similar functions.Below with reference to attached
The embodiment of figure description is exemplary, it is intended to is used to explain the present invention, and is not considered as limiting the invention.
The lateral flow based on centrifugal type microfludic system for describing to propose according to embodiments of the present invention with reference to the accompanying drawings is exempted from
Epidemic disease detection system.
Fig. 1 is the structure of the lateral flow immune detection system based on centrifugal type microfludic system of one embodiment of the invention
Schematic diagram.
As shown in Figure 1, being somebody's turn to do the lateral flow immune detection system based on centrifugal type microfludic system, centrifugal type microfludic system
Including centrifugal type microfludic chip, wherein lateral flow immune detection system 100 includes: lateral flow immune response film 110, lateral
Flow immune detection chip 120 and centrifugal control device 130.
Wherein, centrifugal type microfludic chip upstream reaction cavity and downstream of the lateral flow immune response film 110 for connection
Reaction cavity, and liquid is adjusted by regulation centrifugal rotational speed and flows through the flow velocity that lateral flow is immunoreacted film.Lateral flow immune detection
120 lateral flow immune detection chip of chip is by sample-adding chamber, reaction chamber, lateral flow reaction film pipeline, waste liquid pool and microchannel
Composition, for carrying out default sample process to target sample and detecting the treatment process of target sample.Centrifugal control device 130
For carrying out rotational speed regulation, positioning and imaging to lateral flow immune detection chip 120.The immune inspection of the lateral flow of the embodiment of the present invention
Lateral flow immunoassay technology is able to achieve sample volume, reaction by examining system 100 together with centrifugal type microfludic Integration ofTechnology
Controllable lateral flow immune detection that flow velocity is adjustable, realizes higher detection effect.
It is understood that the purpose of the present invention is be based on centrifugal type microfludic platform, provide a kind of sample volume it is adjustable,
The controllable visualization lateral flow immune detection system of flow velocity, improves the sensitivity of detection.
Specifically, the embodiment of the present invention proposes the arc knot of a reaction film 110 for lateral flow immune detection
Structure, can be connected to upstream and downstream reaction cavity and as response matrix in centrifugal type microfludic chip, and pass through regulation centrifugal rotational speed
Adjust the flow velocity that liquid flows through the reaction film.
The embodiment of the present invention proposes a lateral flow immune detection chip 120 based on centrifugal type microfludic platform, both
The advantages of fluid handling of traditional microfluidic platform is utilized, and combines lateral flow immune detection.The chip is by being loaded
Chamber, reaction chamber, lateral flow reaction film pipeline, waste liquid pool and microchannel composition, can integrate simple sample handle with it is subsequent
Detection process.
The embodiment of the present invention proposes a centrifugal control device 130 based on imaging device visible, the dress
It sets and is made of imaging device, imaging device pallet, imaging chamber, LED plane light source, chip tray, photoelectric door, motor, pedestal etc.,
Under external power supply and control unit manipulation, rotational speed regulation, positioning and imaging can be carried out to chip.
The lateral flow immune detection system 100 based on centrifugal type microfludic system is explained in detail below in conjunction with attached drawing
It states.
Further, in one embodiment of the invention, centrifugal control device 130 is by imaging device, imaging device
Pallet, imaging chamber, LED plane light source, chip tray, photoelectric door, motor, pedestal are constituted.
It should be noted that imaging device can be mobile phone, or with others the imaging devices containing camera, ability
Field technique personnel can select specific imaging device according to actual use demand, and the embodiment of the present invention takes the mobile phone as an example
It is bright.
Specifically, as shown in Fig. 2, the centrifugal control device 130 that the present invention is based on, including mobile phone 11, mobile phone seat
Disk 12, imaging chamber 13, LED plane light source 14, chip tray 15, photoelectric door 16, motor and pedestal 17.The device 130 is in outside
Under power supply and control unit operation, support chip is embedded on chip tray and carries out centrifugally operated.It is grasped by the adjusting of revolving speed etc.
The progress reacted on control chip.Location hole cooperation photoelectric door on chip tray realizes the positioning of chip, and is imaged by mobile phone
Obtain final visual test result.
Furthermore, as shown in figure 3, the immune response film 120 of lateral flow designed by the embodiment of the present invention is arch knot
Structure, has drawn detection line 21, nature controlling line 22 thereon, and both ends are respectively feed liquor area 23, go out liquid zone 24.Reaction film is placed in chip reaction film
In cavity, during centrifugally operated, after reaction film feed liquor area haptoreaction liquid, reaction solution by contrary centrifugal force and
Capillary force comprehensive function is being greater than centrifugal force compared with capillary force under the slow-speed of revolution, and liquid crosses arch along the downward downstream of reaction film
Behind top, liquid can persistently throw away to maintain the capillary force to upstream liquid under the action of the centrifugal force.Change the convection current of revolving speed energy
The flow velocity of reacted film liquid body carries out active control, to optimize lateral flow immunoreaction process.In addition, in centrifugal type microfludic
The liquid product that reaction film is flowed through in chip is also no longer limited by test strips structure, the i.e. controllable reaction liquid product of regulating time.Stream
The active control of speed and volume improves lateral flow sensitivity of immune detection.
Below with reference to embodiment, the invention will be further described, but not as a limitation of the invention.Following embodiments
For the application of several lateral flow immune detection systems in centrifugal type microfludic system, used chip unless otherwise specified,
Using polymethyl methacrylate as material, it is packaged with one side glue.
Further, in one embodiment of the invention, solid-state is previously stored in lateral flow immune detection chip 120
Reagent, wherein solid state reagents enter lateral flow immune response film 110 for reacting after mixing with target sample.
It should be noted that solid state reagents can be freeze-dried reagent, it is of course also possible to stored for other solid-state forms,
And the active reagent of target sample can be maintained, those skilled in the art can select specific solid-state to try according to the actual situation
Agent is not specifically limited herein, and the embodiment of the present invention will be illustrated by taking freeze-dried reagent as an example.
For example, freeze-dried reagent is the reactant being stored in advance in lateral flow immune detection chip 120, it is to be mixed with sample
Approaching side is immunoreacted film 110 to stream and is used for reaction together afterwards, it should be noted that the first functional module to the 4th function mould
Block is respectively arranged with the freeze-dried reagent.
Further, in one embodiment of the invention, lateral flow immune detection chip further includes the first functional module,
Wherein, the first functional module is by the first sample addition zone, the first reaction chamber, the first reaction film cavity, the first waste liquid chamber, the first micro-pipe
Road, sample holes and the first venthole composition, for carrying out default sample process to plasma sample and detecting the processing of plasma sample
Process.
Wherein, reaction was completed after the first preset rotation speed and the first preset time for plasma sample for default sample process, or
Reaction was completed after the second preset rotation speed and the second preset time for plasma sample, wherein it is default that the first preset rotation speed is greater than second
Revolving speed, the first preset time is less than the second preset time.
It is understood that the targeted target sample of the first functional module of the embodiment of the present invention is simplest sample
This, in the first functional module, used in the embodiment of the present invention is by pretreated plasma sample.
Specifically, embodiment one: centrifugal type microfludic lateral flow immune detection (condition 1)
As shown in figure 4, being the chip structure schematic diagram in the present embodiment.First functional module is by the first sample addition zone 31, and
One reaction chamber 32, the first reaction film cavity 33, the first waste liquid chamber 34, the first microchannel 35 and the first sample holes/venthole 36.Core
Freeze-dried reagent is stored in the first sample addition zone 31 by piece using preceding, then is encapsulated with one side glue.In use, by solution to be checked and buffering
The first sample addition zone 31 is added in liquid mixing, and chip is fixed on chip tray 15.In centrifuge high-speed rotation, reaction solution warp
It crosses the first microchannel 35 to contact into the first reaction chamber 32 with reaction film in the first reaction film cavity 33, under 2000rpm revolving speed
Reacting 15min (condition 1), reaction was completed afterwards, is imaged by mobile phone and obtains testing result.
This programme effect:
Fig. 5 a shows reaction solution volume in test strips or flows through and change over time curve on centrifugal lateral flow chip.
The active fluid control of centrifugal lateral flow chip is more stable relative to the passively control of test strips.
Fig. 5 b shows test strips and centrifugal lateral flow chip after the same reaction liquid of different volumes sufficiently reacts
Signal strength comparison.Signal strength on centrifugal lateral flow chip under same reaction liquid product is apparently higher than test strips,
And this programme is without maximum reaction solution volumetric constraint.
Fig. 6 a shows handling of the centrifugal lateral flow chip with rotation speed change to reaction flow velocity.Pass through adjusting
Revolving speed, the flow velocity that reaction solution flows through reaction film is adjustable.
Fig. 6 b show same reaction liquid product under, signal obtained under centrifugal lateral flow chip different rotating speeds
Strength Changes.Different rotating speeds are closely related with testing result.
Embodiment two: centrifugal type microfludic lateral flow immune detection (condition 2)
Operation is with embodiment 1 before reaction solution is contacted with reaction film, after reaction solution and reaction film contact, turns in 1500rpm
Reaction was completed afterwards for lower reaction 45min (condition 2) of speed, is imaged by mobile phone and obtains testing result.
Further, in one embodiment of the invention, lateral flow immune detection chip further includes the second functional module,
Wherein, the second functional module is turned by the second sample addition zone, the separation of whole blood haemocyte and quantitative cavity, buffer Sample application room, buffer
Move room, the first reagent cryodesiccation chamber, the second reaction chamber, the second reaction film cavity, second the second microchannel of waste liquid chamber and the second ventilation
Hole composition, for carrying out default sample process to whole blood sample and detecting the treatment process of whole blood sample.
Specifically, embodiment three: the application of tumor-marker analyte detection in whole blood
Fig. 7 is the chip structure schematic diagram in the present embodiment.Second functional module is by the second sample addition zone 41, whole blood haemocyte
Separation and quantitative cavity 42, buffer Sample application room 43, buffer transfer chamber 44, the first reagent cryodesiccation chamber 45, the second reaction chamber 46,
Second reaction film cavity 47, the second waste liquid chamber 48, the second microchannel 49 and the second venthole 50 composition.In use, whole blood is added
Enter in the second sample addition zone 41, buffer is added in buffer Sample application room 43, chip is fixed on chip tray.In centrifuge height
When speed rotation, whole blood and buffer enter downstream cavity respectively along microchannel 49a.Whole blood haemocyte during high speed centrifugation
It is gradually precipitated to and is sufficiently separated.Revolving speed is reduced, liquid fills hydrophilic siphon piping 49b, increases speed again, blood plasma and buffer
Enter the first reagent cryodesiccation chamber 45 simultaneously mix with reagent, again pass by low high revolving speed, reaction solution into the second reaction chamber 46 and
Reaction film contacts in second reaction film cavity 47, terminates under suitable conditions to reaction, is imaged by mobile phone and obtains testing result.
Further, in one embodiment of the invention, lateral flow immune detection chip further includes third functional module,
Wherein, third functional module is separated by solid-liquid separation and quantitative cavity, the second reagent cryodesiccation chamber, third by third sample addition zone, food homogenate
Reaction chamber, third reaction film cavity, third waste liquid chamber, third microchannel and third venthole composition, for being homogenized sample to food
This carries out default sample process and detects the treatment process of food homogenate sample.
Specifically, example IV: the application of enterotoxin detection in food homogenate
Fig. 8 is the chip structure schematic diagram in the present embodiment.For third functional module by third sample addition zone 51, food homogenate is solid
Liquid separation and quantitative cavity 52, the second reagent cryodesiccation chamber 53, third reaction chamber 54, third reaction film cavity 55, third waste liquid chamber
56, third microchannel 57 and third venthole 58 form.In use, third sample-adding is added in the food homogenate for adding buffer to prepare
Room 51, chip is fixed on chip tray.In centrifuge high-speed rotation, food homogenate enters separation and quantitative cavity 52.It is even
Solid residue in slurry is gradually precipitated under high speed centrifugation to be sufficiently separated.Revolving speed is reduced, liquid fills hydrophilic siphon piping
57b increases speed again, and blood plasma enters the second reagent cryodesiccation chamber 53 simultaneously with buffer and mixes with reagent, again passes by low height
Revolving speed, reaction solution enter third reaction chamber 54 by hydrophilic siphon piping 57c and contact with reaction film, under suitable conditions to reaction
Terminate, is imaged by mobile phone and obtains testing result.
Further, in one embodiment of the invention, lateral flow immune detection chip further includes the 4th functional module,
Wherein, the 4th functional module is by sample suction pipeline, the separation of whole blood haemocyte and quantitative cavity, buffer chamber, mixing chamber, distribution pipe
Road, third reagent cryodesiccation chamber, the 4th reaction chamber, the 4th reaction film cavity, the 4th waste liquid chamber, the 4th microchannel composition, for pair
Finger tip blood sample carries out default sample process and detects the treatment process of finger tip blood sample.
Specifically, embodiment five: the application of immune detection index joint inspection in finger tip blood
Fig. 9 is the chip structure schematic diagram in the present embodiment.4th functional module is by sample suction pipeline 61, whole blood haemocyte point
From with quantitative cavity 62, buffer chamber 63, mixing chamber 64, distribution duct 65, third reagent cryodesiccation chamber 66, the 4th reaction chamber 67,
4th reaction film cavity 68, the 4th waste liquid chamber 69, the equal composition of the 4th microchannel 70.In use, being added in buffer chamber 63 slow
Fliud flushing draws finger tip blood by sample suction pipeline 61, chip is fixed on chip tray.In centrifuge high-speed rotation, whole blood
It is sufficiently separated into the separation of whole blood haemocyte and quantitative cavity 62 to haemocyte and blood plasma.Reduce revolving speed, blood plasma and buffer point
Hydrophilic modification pipeline is not filled up, improves revolving speed, blood plasma and buffer enter the 4th mixing chamber 64, and fill up by distribution duct 65
Each third reagent cryodesiccation chamber 66.Improve revolving speed again, reaction solution break through the superfine microchannel in downstream enter the 4th reaction chamber 66 with
Reaction film contact, terminates to reaction under suitable conditions, is imaged by mobile phone and obtains testing result.
The lateral flow immune detection system based on centrifugal type microfludic system proposed according to embodiments of the present invention, based on from
Core type microfluidic platform provides the visualization lateral flow immune detection system that a kind of sample volume is adjustable, flow velocity is controllable, improves inspection
The sensitivity of survey, by lateral flow immunoassay technology together with centrifugal type microfludic Integration ofTechnology, to be able to achieve sample body
Product, the reaction adjustable controllable lateral flow immune detection of flow velocity, realize higher detection effect.
In addition, term " first ", " second " are used for descriptive purposes only and cannot be understood as indicating or suggesting relative importance
Or implicitly indicate the quantity of indicated technical characteristic.Define " first " as a result, the feature of " second " can be expressed or
Implicitly include at least one this feature.In the description of the present invention, the meaning of " plurality " is at least two, such as two, three
It is a etc., unless otherwise specifically defined.
In the present invention unless specifically defined or limited otherwise, fisrt feature in the second feature " on " or " down " can be with
It is that the first and second features directly contact or the first and second features pass through intermediary mediate contact.Moreover, fisrt feature exists
Second feature " on ", " top " and " above " but fisrt feature be directly above or diagonally above the second feature, or be merely representative of
First feature horizontal height is higher than second feature.Fisrt feature can be under the second feature " below ", " below " and " below "
One feature is directly under or diagonally below the second feature, or is merely representative of first feature horizontal height less than second feature.
In the description of this specification, reference term " one embodiment ", " some embodiments ", " example ", " specifically show
The description of example " or " some examples " etc. means specific features, structure, material or spy described in conjunction with this embodiment or example
Point is included at least one embodiment or example of the invention.In the present specification, schematic expression of the above terms are not
It must be directed to identical embodiment or example.Moreover, particular features, structures, materials, or characteristics described can be in office
It can be combined in any suitable manner in one or more embodiment or examples.In addition, without conflicting with each other, the skill of this field
Art personnel can tie the feature of different embodiments or examples described in this specification and different embodiments or examples
It closes and combines.
Although the embodiments of the present invention has been shown and described above, it is to be understood that above-described embodiment is example
Property, it is not considered as limiting the invention, those skilled in the art within the scope of the invention can be to above-mentioned
Embodiment is changed, modifies, replacement and variant.
Claims (10)
1. a kind of lateral flow immune detection system based on centrifugal type microfludic system, which is characterized in that the centrifugal miniflow
Control system includes centrifugal type microfludic chip, wherein the lateral flow immune detection system includes:
Lateral flow is immunoreacted film, for being connected to the centrifugal type microfludic chip upstream reaction cavity and downstream reaction chamber
Body, and liquid is adjusted by regulation centrifugal rotational speed and flows through the flow velocity that the lateral flow is immunoreacted film；
Lateral flow immune detection chip, the lateral flow immune detection chip is by sample-adding chamber, reaction chamber, lateral flow reaction film
Pipeline, waste liquid pool and microchannel composition, for carrying out default sample process to target sample and detecting the place of the target sample
Reason process；
Centrifugal control device, for carrying out rotational speed regulation, positioning and imaging to the lateral flow immune detection chip.
2. the lateral flow immune detection system according to claim 1 based on centrifugal type microfludic system, which is characterized in that
The lateral flow immune response film is arcuate structure.
3. the lateral flow immune detection system according to claim 1 based on centrifugal type microfludic system, which is characterized in that
Centrifugal control device is by imaging device, imaging device pallet, imaging chamber, LED plane light source, chip tray, photoelectric door, electricity
Machine, pedestal are constituted.
4. the lateral flow immune detection system according to claim 1 based on centrifugal type microfludic system, which is characterized in that
Solid state reagents are previously stored in the lateral flow immune detection chip, wherein the solid state reagents and the target sample are mixed
Enter lateral flow immune response film after conjunction for reacting.
5. the lateral flow immune detection system according to claim 1 or 4 based on centrifugal type microfludic system, feature exist
In the target sample includes plasma sample, whole blood sample, food homogenate sample and finger tip blood sample.
6. the lateral flow immune detection system according to claim 5 based on centrifugal type microfludic system, which is characterized in that
The lateral flow immune detection chip further includes the first functional module, wherein first functional module is by the first sample addition zone,
One reaction chamber, the first reaction film cavity, the first waste liquid chamber, the first microchannel, sample holes and the first venthole composition, for institute
Plasma sample is stated to carry out default sample process and detect the treatment process of the plasma sample.
7. the lateral flow immune detection system according to claim 6 based on centrifugal type microfludic system, which is characterized in that
The default sample process is that reaction was completed or the blood after the first preset rotation speed and the first preset time for the plasma sample
Starching sample, reaction was completed after the second preset rotation speed and the second preset time, wherein first preset rotation speed is greater than described the
Two preset rotation speeds, first preset time is less than the second preset time.
8. the lateral flow immune detection system according to claim 5 based on centrifugal type microfludic system, which is characterized in that
The lateral flow immune detection chip further includes the second functional module, wherein second functional module is by the second sample addition zone, complete
The separation of blood haemocyte and quantitative cavity, buffer Sample application room, buffer transfer chamber, the first reagent cryodesiccation chamber, the second reaction chamber, the
Two reaction film cavitys, second the second microchannel of waste liquid chamber and the second venthole composition, for being preset to the whole blood sample
Sample process and the treatment process for detecting the whole blood sample.
9. the lateral flow immune detection system according to claim 5 based on centrifugal type microfludic system, which is characterized in that
The lateral flow immune detection chip further includes third functional module, wherein the third functional module is by third sample addition zone, food
Object homogenate be separated by solid-liquid separation with quantitative cavity, the second reagent cryodesiccation chamber, third reaction chamber, third reaction film cavity, third waste liquid chamber,
Third microchannel and third venthole composition, for carrying out default sample process to food homogenate sample and detecting the food
The treatment process of object homogenate sample.
10. the lateral flow immune detection system according to claim 5 based on centrifugal type microfludic system, feature exist
In the lateral flow immune detection chip further includes the 4th functional module, wherein the 4th functional module is by sample suction pipeline, whole blood
Haemocyte separation and quantitative cavity, buffer chamber, mixing chamber, distribution duct, third reagent cryodesiccation chamber, the 4th reaction chamber, the 4th
Reaction film cavity, the 4th waste liquid chamber, the 4th microchannel composition, for carrying out default sample process and inspection to the finger tip blood sample
Survey the treatment process of the finger tip blood sample.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111537708A (en) * | 2020-06-11 | 2020-08-14 | 烟台芥子生物技术有限公司 | Microfluidic detection structure and application thereof |
| CN111604098A (en) * | 2020-06-04 | 2020-09-01 | 天津德祥生物技术有限公司 | Micro-fluidic chip with side sample adding function |
| CN111855994A (en) * | 2020-07-29 | 2020-10-30 | 成都微康生物科技有限公司 | POCT (point of care testing) immunodetection chip capable of carrying out multiple joint detections on whole blood sample adding at one time |
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| CN111983212A (en) * | 2020-07-21 | 2020-11-24 | 中国科学院长春光学精密机械与物理研究所 | Centrifugal microfluidic detection system and method for seminal plasma fructose |
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| CN113325170A (en) * | 2021-06-16 | 2021-08-31 | 博奥生物集团有限公司 | Test paper chip device, micro-fluidic chip and lateral flow test paper strip |
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| WO2024082564A1 (en) * | 2022-10-19 | 2024-04-25 | 南方科技大学 | Centrifugal micro-fluidic chip |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102177439A (en) * | 2008-10-14 | 2011-09-07 | 三星电子株式会社 | Microfluidic device using centrifugal force and sample analyzing method using the microfluidic device |
| KR20130000009A (en) * | 2011-06-15 | 2013-01-02 | 국립대학법인 울산과학기술대학교 산학협력단 | Microfluidic device and mamufacturing method thereof, apparatus and method detecting specimen using the same |
| CN105353159A (en) * | 2014-08-20 | 2016-02-24 | 余波 | Microfluidic test device and method for operating the same |
| CN105964313A (en) * | 2016-01-22 | 2016-09-28 | 上海快灵生物科技有限公司 | Centrifugal type multi-channel microfluidic chip |
| CN106770294A (en) * | 2016-12-26 | 2017-05-31 | 中国科学院长春光学精密机械与物理研究所 | A kind of centrifugal type microfludic chip, preparation method and its application process |
| CN109283174A (en) * | 2018-09-29 | 2019-01-29 | 厦门大学 | A kind of quantitative detection CD and detection method |
-
2019
- 2019-03-01 CN CN201910156152.3A patent/CN109884328A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102177439A (en) * | 2008-10-14 | 2011-09-07 | 三星电子株式会社 | Microfluidic device using centrifugal force and sample analyzing method using the microfluidic device |
| KR20130000009A (en) * | 2011-06-15 | 2013-01-02 | 국립대학법인 울산과학기술대학교 산학협력단 | Microfluidic device and mamufacturing method thereof, apparatus and method detecting specimen using the same |
| CN105353159A (en) * | 2014-08-20 | 2016-02-24 | 余波 | Microfluidic test device and method for operating the same |
| CN105964313A (en) * | 2016-01-22 | 2016-09-28 | 上海快灵生物科技有限公司 | Centrifugal type multi-channel microfluidic chip |
| CN106770294A (en) * | 2016-12-26 | 2017-05-31 | 中国科学院长春光学精密机械与物理研究所 | A kind of centrifugal type microfludic chip, preparation method and its application process |
| CN109283174A (en) * | 2018-09-29 | 2019-01-29 | 厦门大学 | A kind of quantitative detection CD and detection method |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112756018A (en) * | 2019-10-21 | 2021-05-07 | 广州万孚生物技术股份有限公司 | Micro-fluidic chip and in-vitro detection system |
| CN111604098A (en) * | 2020-06-04 | 2020-09-01 | 天津德祥生物技术有限公司 | Micro-fluidic chip with side sample adding function |
| CN111604098B (en) * | 2020-06-04 | 2022-01-07 | 天津德祥生物技术有限公司 | Micro-fluidic chip with side sample adding function |
| CN111537708A (en) * | 2020-06-11 | 2020-08-14 | 烟台芥子生物技术有限公司 | Microfluidic detection structure and application thereof |
| CN111879761A (en) * | 2020-06-22 | 2020-11-03 | 武汉新烽光电股份有限公司 | Micro-reagent-consumption water quality residual chlorine detection method based on micro-fluidic disc chip |
| CN111983212A (en) * | 2020-07-21 | 2020-11-24 | 中国科学院长春光学精密机械与物理研究所 | Centrifugal microfluidic detection system and method for seminal plasma fructose |
| CN111855994A (en) * | 2020-07-29 | 2020-10-30 | 成都微康生物科技有限公司 | POCT (point of care testing) immunodetection chip capable of carrying out multiple joint detections on whole blood sample adding at one time |
| CN111855994B (en) * | 2020-07-29 | 2021-06-11 | 成都微康生物科技有限公司 | POCT (point of care testing) immunodetection chip capable of carrying out multiple joint detections on whole blood sample adding at one time |
| WO2022052787A1 (en) * | 2020-09-08 | 2022-03-17 | 深圳市亚辉龙生物科技股份有限公司 | Microfluidic immunodetection chip and microfluidic linear immunodetection method |
| CN113325170A (en) * | 2021-06-16 | 2021-08-31 | 博奥生物集团有限公司 | Test paper chip device, micro-fluidic chip and lateral flow test paper strip |
| WO2024082564A1 (en) * | 2022-10-19 | 2024-04-25 | 南方科技大学 | Centrifugal micro-fluidic chip |
| WO2024085844A3 (en) * | 2022-10-20 | 2024-06-06 | Izmir Yuksek Teknoloji Enstitusu Rektorlugu | Closed-channel microfluidic platform working with centrifugal principle |
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