CN111855994B - POCT (point of care testing) immunodetection chip capable of carrying out multiple joint detections on whole blood sample adding at one time - Google Patents
POCT (point of care testing) immunodetection chip capable of carrying out multiple joint detections on whole blood sample adding at one time Download PDFInfo
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- CN111855994B CN111855994B CN202010740918.5A CN202010740918A CN111855994B CN 111855994 B CN111855994 B CN 111855994B CN 202010740918 A CN202010740918 A CN 202010740918A CN 111855994 B CN111855994 B CN 111855994B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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Abstract
The invention discloses a POCT (point of care testing) immunodetection chip capable of realizing multiple joint tests in one-time whole blood sample adding, and solves the technical problem that in the prior art, doctors need to spend too much time and energy on blood sampling detection operation to prevent and treat patients due to the fact that multiple joint tests in one-time whole blood sample adding cannot be realized. The device comprises a sample processing module and a liquid separation reaction module, wherein the sample processing module is used for carrying out centrifugal separation and dilution after separation on a blood sample so as to obtain a plasma blending diluent, and the liquid separation reaction module is used for carrying out fluorescence immunoassay on the plasma blending diluent processed by the sample processing module; the liquid separation reaction module and the sample processing module are connected through pasting, and a flow passage is communicated between the liquid separation reaction module and the sample processing module. The invention can support whole blood detection, carry out high-sensitivity and high-repeatability detection on the multi-target analyte in the same sample, and effectively reduce the frequency of blood sampling detection operation of doctors, thereby saving more time for doctors to carry out first aid on patients.
Description
Technical Field
The invention belongs to the technical field of microfluidic POCT detection equipment, and particularly relates to a disc type microfluidic fluorescence immunoassay chip capable of performing multi-item joint detection on whole blood at one time.
Background
POCT (Point-of-care Testing), also known as Point-of-care Testing, or on-site rapid Testing, refers to a Testing method performed on a sampling site and using a portable analyzer and a matching reagent to obtain a Testing result rapidly. In view of the current market situation, the detection system based on the immunochromatography technology is most applied, is light, simple and low-cost, and has cheap consumables, so that the detection system is favored by primary hospitals. With the implementation of a national classification diagnosis and treatment system, measures such as the first diagnosis of the primary level, the first treatment of the emergency, the second treatment of the emergency and the like put higher demands on the instant detection, particularly, the emergency patients have a plurality of diagnosis and detection items, the detection system is adopted for detection, as each detection item needs to be independently used for blood sampling and detection, a large number of detection operations need to be repeated for the multi-item detection, and the patients need to be treated by doctors in a time-consuming manner due to the illness state, and the excessive detection operations can become tragus stones for the doctors to treat the patients.
Therefore, the design of a POCT immunoassay chip can support whole blood detection, and can perform multi-project combined detection by sample addition at one time, so as to reduce the frequency of blood sampling detection operations of doctors, thereby saving more time for doctors to perform emergency treatment on patients, and the POCT immunoassay chip becomes a technical problem to be solved by technical personnel in the technical field.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: the POCT immune detection chip capable of realizing once whole blood sample adding and multi-item joint inspection is provided, and the technical problem that in the prior art, doctors need to spend too much time and energy to perform blood sampling detection operation to prevent and cure patients due to the fact that once whole blood sample adding and multi-item joint inspection cannot be realized is solved.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
the POCT immune detection chip capable of carrying out multiple joint inspection on whole blood sample at one time comprises a sample processing module and a liquid separation reaction module, wherein the sample processing module is used for carrying out centrifugal separation and dilution after separation on a blood sample to obtain a plasma blending diluent, and the liquid separation reaction module is used for carrying out fluorescence immune detection on the plasma blending diluent processed by the sample processing module; the liquid separation reaction module and the sample processing module are connected through pasting, and a flow passage is communicated between the liquid separation reaction module and the sample processing module.
The sample processing module comprises a disc-shaped sample processing layer, a processing chamber for centrifugally separating and diluting a blood sample is arranged on the sample processing layer, and a sample processing layer sealing plate for sealing the processing chamber is arranged on the sample processing layer; preferably, ultrasonic welding ribs are arranged in the processing chamber, and the sample processing layer sealing plate and the ultrasonic welding ribs are fixed through ultrasonic welding; preferably, the sample processing layer is provided with a rotary positioning groove.
Furthermore, a sample loading bin for adding a blood sample, a plasma bin body communicated with the sample loading bin and used for containing plasma separated from the blood sample, a diluent loading bin for adding a diluent, and a diluting and mixing bin which is respectively connected with the plasma bin body and the diluent loading bin and is commonly used for mixing the plasma and the diluent into a plasma mixing diluent are arranged in the processing chamber; the sample processing layer is provided with a plurality of plasma blending liquid output ports which are distributed around the processing chamber at equal intervals and used for conveying plasma blending diluent to the liquid separation reaction module, the diluting and blending bin is communicated with a sample distribution channel corresponding to the plasma blending liquid output port, the plasma blending diluent in the diluting and blending bin flows to the plasma blending liquid output port through the sample distribution channel, and the sample distribution channel is preferably a siphon channel and can also be driven by an air pump or other power; preferably, the sample processing sealing plate is provided with a dilution and mixing bin vent hole communicated with the dilution and mixing bin.
Further, the sample adding bin and the plasma bin body are communicated through a centrifugal separation channel; preferably, a blood cell cabin body which is connected with the sample loading bin through a centrifugal separation channel and is commonly used for containing blood cells separated from the blood sample is arranged in the processing chamber; preferably, a sample quantitative waste liquid bin communicated with the plasma bin body and used for containing redundant blood samples is further arranged in the processing chamber; further preferably, the sample processing layer closing plate is provided with a sample quantitative waste liquid bin vent hole communicated with the sample quantitative waste liquid bin.
Furthermore, the plasma chamber body and the diluting and mixing chamber are communicated through a plasma diluting channel, and the plasma diluting channel is preferably a siphon flow channel and can also be driven by an air pump or other power.
Furthermore, a diluent buffer bin communicated with the diluent sample adding bin through a diluent buffer channel is also arranged in the processing chamber, and the diluent buffer bin is communicated with the diluting and uniformly mixing bin; preferably, the diluent buffer storage bin is communicated with the diluent mixing bin through a diluent channel; further preferably, the diluent channel is a siphon channel, and the sample processing sealing plate is provided with a diluent buffer bin vent hole communicated with the diluent buffer bin.
Further, be equipped with sample waste liquid storehouse on the sample processing layer to it has sample waste liquid storehouse passageway to dilute to communicate between mixing storehouse and the sample waste liquid storehouse.
Furthermore, a sample loading buffer bin and a diluent loading buffer bin are arranged on the sample processing layer sealing plate, the sample loading buffer bin is communicated with the sample loading bin through a sample loading hole, and the diluent loading buffer bin is communicated with the diluent loading bin through a diluent loading hole.
Further, divide liquid reaction module including being discoid branch liquid reaction layer, divide liquid reaction layer in and be equipped with a plurality of reaction detection room, divide liquid reaction layer to be equipped with and be used for the encapsulation reaction to detect the branch liquid reaction layer shrouding of room.
Furthermore, a sample quantifying bin, a blending reaction bin, a test strip placing groove and a water-absorbent paper placing groove which are sequentially communicated are arranged in the reaction detection chamber, the sample quantifying bin is provided with a sample quantifying bin inlet communicated with the sample processing module, a reaction reagent is arranged in the blending reaction bin, the reaction reagent is preferably a freeze-dried reagent ball, a test strip is arranged in the test strip placing groove, water-absorbent paper is arranged in the water-absorbent paper placing groove, and a detection window corresponding to the test strip placing groove is formed in the liquid separation reaction layer; preferably, the liquid separation reaction layer is provided with uniform mixing reaction bin air vents corresponding to the uniform mixing reaction bins; preferably, the sample quantitative bin is communicated with the uniformly mixing reaction bin through a reaction siphon channel; preferably, the mixing reaction bin is communicated with the test strip placing groove through a capillary capture channel.
Further, a plasma blending diluent conveying hole is formed in the liquid separation reaction layer sealing plate, and the plasma blending diluent in the sample processing module enters the reaction detection chamber of the liquid separation reaction module through the plasma blending diluent conveying hole; preferably, a sealing plate sample adding yielding hole is arranged on the liquid separating reaction layer sealing plate; preferably, a sample loading yielding hole corresponding to the sample loading buffer bin and a diluent loading yielding hole corresponding to the diluent loading buffer bin are arranged on the liquid separation reaction layer.
Further, a chip rotating positioning groove is arranged on the liquid separation reaction layer; preferably, the liquid separation reaction layer sealing plate is provided with a sealing plate positioning groove corresponding to the chip rotation positioning groove.
Further, the liquid separation reaction layer sealing plate is a thick base material single-sided adhesive, a thick base material double-sided adhesive or a plastic plate adhered with the double-sided adhesive; preferably, when the liquid separation reaction layer sealing plate is a plastic plate adhered with a double-sided adhesive tape, the plastic plate is fixed with the liquid separation reaction layer through ultrasonic welding or laser welding.
Compared with the prior art, the invention has the following beneficial effects:
the invention has simple structure, scientific and reasonable design and convenient use, is a chip for realizing high-sensitivity and high-repeatability detection of multiple target analytes in the same sample by combining a whole blood separation technology, a microfluidic technology, a spray freeze drying technology, a fluorescence labeling technology, an immunoreaction technology and a flow capture technology, can support whole blood detection, and can carry out combined detection of multiple items by once sample application so as to reduce the frequency of blood sampling detection operation of doctors, thereby saving more time for doctors to carry out emergency treatment on patients. Can be applied to the fields of biomedical research, clinical diagnosis, judicial identification and the like.
Drawings
Fig. 1 is a schematic diagram of an explosive structure according to the present invention.
Fig. 2 is a structural diagram of a bin body flow channel inside the sample processing module.
Fig. 3 is a schematic view of the sample processing layer sealing plate of the present invention being encapsulated on the sample processing layer.
Fig. 4 is a structure diagram of a bin body flow channel inside the liquid-separating reaction module.
FIG. 5 is a schematic diagram of a liquid separation reaction module according to the present invention.
FIG. 6 is a schematic view of the present invention.
FIG. 7 is a schematic view of another aspect of the present invention.
Wherein, the names corresponding to the reference numbers are:
1. a sample processing layer; 2. a sample processing layer sealing plate; 3. a liquid separation reaction layer sealing plate; 4. absorbent paper; 5. a test strip; 6. separating a liquid reaction layer; 7. a reaction reagent; 8. a sample processing module; 9. a liquid separation reaction module; 1-1, sample loading bin; 1-2, a centrifugal separation channel; 1-3, a blood cell cabin body; 1-4, a plasma bin body; 1-5, a sample quantitative waste liquid bin; 1-6, a plasma dilution channel; 1-7, a diluting and uniformly mixing bin; 1-8, sample distribution channels; 1-9, a blood plasma mixing liquid outlet; 1-10, a sample waste liquid bin channel; 1-11, a sample waste liquid bin; 1-12, a diluent sample feeding bin; 1-13, a diluent buffer channel; 1-14, a diluent buffer bin; 1-15, a diluent channel; 1-16, ultrasonically welding ribs; 1-17, rotating positioning grooves; 2-1, sample addition holes; 2-2, adding a sample hole for diluent; 2-3, ventilating holes of a diluent buffer storage bin; 2-4, air holes of a sample quantitative waste liquid bin; 2-5, diluting and uniformly mixing air holes in a bin; 2-6, a sample loading and buffering bin; 2-7, adding a diluent into a sample buffering bin; 3-1, a plasma mixing diluent delivery hole; 3-2, sealing plate sample adding and yielding holes; 3-3, sealing plate positioning grooves; 6-1, a sample quantification bin inlet; 6-2, a sample quantification bin; 6-3, a reaction siphon channel; 6-4, a uniformly mixing reaction bin; 6-5, uniformly mixing air holes in the reaction bin; 6-6, capillary capture channels; 6-7, a test strip placing groove; 6-8, detecting a window; 6-9, placing a water absorption paper placing groove; 6-10, chip rotary positioning grooves; 6-11, sample adding and yielding holes; 6-12, and adding and yielding the diluent.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to the accompanying drawings. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation or be constructed and operated in a particular orientation, and thus, it should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; of course, mechanical connection and electrical connection are also possible; alternatively, they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
As shown in fig. 1 to 7, the disc-type microfluidic fluorescence immunoassay chip capable of performing multiple joint detections on whole blood at one time provided by the invention realizes a chip for performing high-sensitivity and high-repeatability detection on multiple target analytes in the same sample by combining a whole blood separation technology, a microfluidic technology, a spray freeze drying technology, a fluorescence labeling technology, an immunoreaction technology and a flow capture technology, can be applied to the fields of biomedical research, clinical diagnosis, judicial identification and the like, and belongs to the field of microfluidic POCT detection. The invention comprises a sample processing module 8 for carrying out centrifugal separation and dilution after separation on a blood sample to obtain a plasma blending diluent, and a liquid separation reaction module 9 for carrying out fluorescence immunoassay on the plasma blending diluent processed by the sample processing module 8; the liquid separation reaction module 9 and the sample processing module 8 are connected by adhesion, and a flow channel is communicated between the two.
The sample processing module 8 comprises a disc-shaped sample processing layer 1, a processing chamber for centrifugally separating and diluting a blood sample is arranged on the sample processing layer 1, and a sample processing layer sealing plate 2 for sealing the processing chamber is arranged on the sample processing layer 1; preferably, ultrasonic welding ribs 1-16 are arranged in the processing chamber, and the sample processing layer sealing plate 2 and the ultrasonic welding ribs 1-16 are fixed through ultrasonic welding; preferably, the sample processing layer 1 is provided with rotary positioning grooves 1-17. A sample loading bin 1-1 for adding a blood sample, a plasma bin body 1-4 which is communicated with the sample loading bin 1-1 and is used for containing plasma separated from the blood sample, a diluent loading bin 1-12 for adding a diluent, and a diluting and uniformly mixing bin 1-7 which is respectively connected with the plasma bin body 1-4 and the diluent loading bin 1-12 and is commonly used for uniformly mixing the plasma and the diluent into a plasma uniformly mixing diluent are arranged in the processing chamber; a plurality of plasma blending liquid output ports 1-9 which are distributed around the treatment chamber at equal intervals and used for conveying plasma blending diluent to the liquid separation reaction module 9 are arranged on the sample treatment layer 1, sample distribution channels 1-8 corresponding to the plasma blending liquid output ports 1-9 are communicated with the dilution blending chambers 1-7, and the plasma blending diluent in the dilution blending chambers 1-7 flows to the plasma blending liquid output ports 1-9 through the sample distribution channels 1-8; preferably, the sample processing sealing plate 2 is provided with air holes 2-5 of the diluting and mixing bin communicated with the diluting and mixing bins 1-7.
The sample adding bin 1-1 and the plasma bin 1-4 are communicated through a centrifugal separation channel 1-2; preferably, a blood cell cabin body 1-3 which is connected with the sample loading cabin 1-1 through a centrifugal separation channel 1-2 and is commonly used for containing blood cells separated from a blood sample is arranged in the processing chamber; preferably, a sample quantitative waste liquid bin 1-5 which is connected with the plasma bin body 1-4 and is used for containing redundant blood samples is also arranged in the processing chamber; further preferably, the sample processing sealing plate 2 is provided with sample quantitative waste liquid bin air vents 2-4 communicated with the sample quantitative waste liquid bins 1-5. The plasma chamber body 1-4 is communicated with the diluting and mixing chamber 1-7 through the plasma diluting channel 1-6. The plasma bin body 1-4 is positioned between the sample loading bin body 1-1 and the blood cell bin body 1-3, and the sample quantitative waste liquid bin 1-5 is positioned at the side parts of the plasma bin body 1-4 and the blood cell bin body 1-3.
The treatment chamber is also internally provided with diluent buffer bins 1-14 communicated with diluent sample adding bins 1-12 through diluent buffer channels 1-13, and the diluent buffer bins 1-14 are communicated with diluting and uniformly mixing bins 1-7; preferably, the diluent buffer bins 1-14 are communicated with the diluent mixing bins 1-7 through diluent channels 1-15; further preferably, the diluent channel 1-15 is a siphon channel, the sample processing sealing plate 2 is provided with a diluent buffer bin vent hole 2-3 communicated with the diluent buffer bin 1-14, and if the diluent channel 1-15 is driven in a pneumatic mode, the sample processing sealing plate 2 is provided with an air pump interface communicated with the diluent buffer bin 1-14. The sample processing layer 1 is provided with sample waste liquid bins 1-11, and sample waste liquid bin channels 1-10 are communicated between the diluting and uniformly mixing bins 1-7 and the sample waste liquid bins 1-11.
The sample processing sealing plate 2 is provided with a sample loading buffer bin 2-6 and a diluent loading buffer bin 2-7, the sample loading buffer bin 2-6 is communicated with the sample loading bin 1-1 through a sample loading hole 2-1, and the diluent loading buffer bin 2-7 is communicated with the diluent loading bin 1-12 through a diluent loading hole 2-2.
The liquid separation reaction module 9 comprises a disc-shaped liquid separation reaction layer 6, a plurality of reaction detection chambers are arranged in the liquid separation reaction layer 6, and the liquid separation reaction layer 6 is provided with a liquid separation reaction layer sealing plate 3 for packaging the reaction detection chambers.
A sample quantifying bin 6-2, a blending reaction bin 6-4, a test strip placing groove 6-7 and a water-absorbing paper placing groove 6-9 which are sequentially communicated are arranged in a reaction detection chamber, the sample quantifying bin 6-2 is provided with a sample quantifying bin inlet 6-1 communicated with a sample processing module 8, a reaction reagent 7 is arranged in the blending reaction bin 6-4, a test strip 5 is arranged in the test strip placing groove 6-7, water-absorbing paper 4 is arranged in the water-absorbing paper placing groove 6-9, and a detection window 6-8 corresponding to the test strip placing groove 6-7 is arranged on a liquid separation reaction layer 6; preferably, the liquid separation reaction layer 6 is provided with uniform mixing reaction bin air holes 6-5 corresponding to the uniform mixing reaction bins 6-4; preferably, the sample quantitative bin 6-2 is communicated with the uniform mixing reaction bin 6-4 through a reaction siphon channel 6-3; preferably, the mixing reaction chamber 6-4 is communicated with the test strip placing groove 6-7 through a capillary capture channel 6-6.
A plasma blending diluent conveying hole 3-1 is formed in the liquid separation reaction layer sealing plate 3, and plasma blending diluent in the sample processing module 8 enters a reaction detection chamber of the liquid separation reaction module 9 through the plasma blending diluent conveying hole 3-1; preferably, a sealing plate sample adding yielding hole 3-2 is arranged on the liquid separating reaction layer sealing plate 3; preferably, the liquid separation reaction layer 6 is provided with sample loading yielding holes 6-11 corresponding to the sample loading buffer bins 2-6 and diluent loading yielding holes 6-12 corresponding to the diluent loading buffer bins 2-7.
The liquid separation reaction layer 6 is provided with chip rotary positioning grooves 6-10; preferably, the liquid separation reaction layer sealing plate 3 is provided with sealing plate positioning grooves 3-3 corresponding to the chip rotation positioning grooves 6-10. The liquid separation reaction layer sealing plate 3 is a thick base material single-sided adhesive, a thick base material double-sided adhesive or a plastic plate adhered with the double-sided adhesive; preferably, when the liquid separation reaction layer sealing plate 3 is a plastic plate adhered with a double-sided adhesive tape, the plastic plate is fixed with the liquid separation reaction layer 6 by ultrasonic welding or laser welding.
The invention has simple structure, scientific and reasonable design and convenient use, is a chip for realizing high-sensitivity and high-repeatability detection of multiple target analytes in the same sample by combining a whole blood separation technology, a microfluidic technology, a spray freeze drying technology, a fluorescence labeling technology, an immunoreaction technology and a flow capture technology, can support whole blood detection, and can carry out combined detection of multiple items by once sample application so as to reduce the frequency of blood sampling detection operation of doctors, thereby saving more time for doctors to carry out emergency treatment on patients. Can be applied to the fields of biomedical research, clinical diagnosis, judicial identification and the like.
The sample processing layer 1, the sample processing layer sealing plate 2, the liquid separation reaction layer sealing plate 3 and the liquid separation reaction layer 6 are not provided with special precise structures, can be molded by injection by a common injection molding technology, do not need complex chip bioactive substance coating, and have low cost and high yield.
The POCT immune detection chip of the invention is formed by a sample processing layer 1 and a sample processing layer seal plate 2 through ultrasonic welding to form a sample processing module 8, and a liquid separation reaction layer 6 is formed by ultrasonic welding with a liquid separation reaction layer seal plate 3 after a corresponding test strip 5, a reaction reagent 7 and a water absorption paper 4 are assembled. Divide liquid reaction layer shrouding 3 can be the one-sided glue or the double faced adhesive tape of thick substrate, also can be the plastic slab and laminate the double faced adhesive tape after the welding, and sample processing module 8 becomes final dish formula micro-fluidic fluorescence immunoassay chip with dividing liquid reaction module 9 through dividing the gluing on the liquid reaction layer shrouding 3 together.
The POCT immune detection chip (hereinafter referred to as chip) is matched with an instrument (patent number: 2020104496995, patent name: a disc microfluidic fluorescence immunoassay analyzer and an immune project testing method) of the company for use, when the POCT immune detection chip is used, a package is unpacked, the chip is horizontally placed on a table, 660 mu L of whole blood sample or serum/plasma sample is added from a sample adding hole 2-1 through a pipette, and then 660 mu L of corresponding diluent is added from a diluent adding hole 2-2. Keeping the chip stably placed in a tray of the detection instrument, then clicking to start testing, waiting for the detection to finish outputting a result, taking out the chip and placing the chip into a medical waste garbage can to finish the whole test.
The whole process of the chip in the detection process inside the instrument is as follows: firstly, the chip rotates at the speed of 4000-. Meanwhile, the diluent in the diluent sample loading bin 1-12 enters the diluent cache bin 1-14 through the diluent cache channel 1-13 under the action of centrifugal force. During the high-speed centrifugation, the plasma dilution channels 1-6 and the diluent channels 1-15 are not communicated due to the centrifugal force.
The two siphoning channels, plasma dilution 1-6 and diluent 1-15, act like two closed valves preventing the flow of liquid. When the chip stops rotating slowly after the high-speed centrifugation is finished, the plasma dilution channels 1-6 and the diluent channels 1-15 are filled with corresponding liquid under the action of capillary force, so that the two siphon channels are communicated, namely two valves are opened. Then the chip is rotated for 8-15s at the speed of 2200-.
When the chip rotates at high speed, the sample distribution channels 1-8 are in a closed state, and after the chip finishes mixing and stops, the sample distribution channels 1-8 are in a communicated state after being filled with mixing liquid under the action of capillary force. Then the chip rotates at the speed of 2200-, after the liquid comes out, the liquid can be accumulated at the outlet for a short time due to certain hysteresis caused by inertia, and after a certain amount of liquid is reached, the liquid enters the nearest inlet and is filled with the next liquid.
After the chip stops rotating, the nine filled sample quantitative bins 6-2 are communicated with the reaction siphon channel 6-3 under the capillary action. And then the chip rotates at the speed of 2200-. After the rotation is stopped, the liquid in the mixing reaction bin 6-4 flows to the test strip 5 placed in the test strip placing groove 6-7 through the capillary capturing channel 6-6 under the capillary action to complete the reaction.
The chip sample processing layer 1, the sample processing layer sealing plate 2, the liquid separation reaction layer sealing plate 3 and the liquid separation reaction layer 6 have no special precise structures, can be molded by injection by a common injection molding technology, do not need complex chip bioactive substance coating, and have low cost and high yield.
The liquid separation reaction layer sealing plate 3 is provided with a plasma blending diluent conveying hole 3-1, and the plasma blending diluent conveying hole 3-1 is respectively and correspondingly communicated with a plasma blending diluent output port 1-9 and a sample quantitative bin inlet 6-1 when the chip is assembled.
Finally, it should be noted that: the above embodiments are only preferred embodiments of the present invention to illustrate the technical solutions of the present invention, but not to limit the technical solutions, and certainly not to limit the patent scope of the present invention; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention; that is, the technical problems to be solved by the present invention, which are not substantially changed or supplemented by the spirit and the concept of the main body of the present invention, are still consistent with the present invention and shall be included in the scope of the present invention; in addition, the technical scheme of the invention is directly or indirectly applied to other related technical fields, and the technical scheme is included in the patent protection scope of the invention.
Claims (17)
1. POCT immunodetection chip that can multiple joint inspection of whole blood application of sample, its characterized in that: the device comprises a sample processing module (8) for performing centrifugal separation and dilution after separation on a blood sample to obtain a plasma blending diluent, and a liquid separation reaction module (9) for performing immunoassay on the plasma blending diluent processed by the sample processing module (8); the liquid separation reaction module (9) is connected with the sample processing module (8), and a flow channel is communicated between the liquid separation reaction module and the sample processing module;
the sample processing module (8) comprises a sample processing layer (1), a processing chamber for performing centrifugal separation and dilution after separation on a blood sample is arranged on the sample processing layer (1), a sample processing layer sealing plate (2) for sealing the processing chamber is arranged on the sample processing layer (1), ultrasonic welding ribs (1-16) are arranged in the processing chamber, and the sample processing layer sealing plate (2) and the ultrasonic welding ribs (1-16) are fixed through ultrasonic welding;
a sample loading bin (1-1) for adding a blood sample is arranged in the processing chamber, a plasma bin body (1-4) which is communicated with the sample loading bin (1-1) and is used for containing plasma separated from the blood sample, a diluent loading bin (1-12) for adding a diluent, and a diluting and mixing bin (1-7) which is respectively connected with the plasma bin body (1-4) and the diluent loading bin (1-12) and is commonly used for mixing the plasma and the diluent into a plasma mixing diluent, a plurality of plasma mixing liquid output ports (1-9) for conveying the plasma mixing diluent to the liquid separation reaction module (9) are arranged on the sample processing layer (1);
a blood cell cabin body (1-3) which is connected with the sample loading cabin (1-1) through a centrifugal separation channel (1-2) and is used for containing hemocytes separated from a blood sample is arranged in the processing chamber, and a sample quantitative waste liquid cabin (1-5) which is connected with the plasma cabin body (1-4) and is used for containing redundant blood samples is also arranged in the processing chamber;
the treatment chamber is also internally provided with a diluent buffer bin (1-14) communicated with a diluent sample adding bin (1-12) through a diluent buffer channel (1-13), and the diluent buffer bin (1-14) is communicated with a diluting and uniformly mixing bin (1-7) through a diluent channel (1-15); the diluent channel (1-15) is a siphon channel, and the sample processing layer sealing plate (2) is provided with diluent buffer bin air holes (2-3) communicated with the diluent buffer bin (1-14);
the sample processing layer sealing plate (2) is provided with a sample loading buffer bin (2-6) and a diluent loading buffer bin (2-7), the sample loading buffer bin (2-6) is communicated with the sample loading bin (1-1) through a sample loading hole (2-1), and the diluent loading buffer bin (2-7) is communicated with the diluent loading bin (1-12) through a diluent loading hole (2-2).
2. The POCT immunodetection chip capable of performing multiple joint tests on whole blood at one time according to claim 1, wherein: the sample processing layer (1) is provided with rotary positioning grooves (1-17).
3. The POCT immunodetection chip capable of performing multiple joint tests on whole blood at one time according to claim 2, wherein: the diluting and mixing bin (1-7) is communicated with a sample distribution channel (1-8) corresponding to the plasma mixing liquid output port (1-9), and plasma mixing diluent in the diluting and mixing bin (1-7) flows to the plasma mixing liquid output port (1-9) through the sample distribution channel (1-8).
4. The POCT immunodetection chip capable of performing multiple joint tests on whole blood at one time according to claim 3, wherein: the sample processing sealing plate (2) is provided with air holes (2-5) of a diluting and mixing bin which are communicated with the diluting and mixing bins (1-7).
5. The POCT immunodetection chip capable of performing multiple joint tests on whole blood at one time according to claim 3, wherein: the sample loading bin (1-1) is communicated with the plasma bin body (1-4) through a centrifugal separation channel (1-2).
6. The POCT immunodetection chip capable of performing multiple joint tests on whole blood at one time according to claim 5, wherein: the plasma chamber body (1-4) is communicated with the diluting and uniformly mixing chamber (1-7) through a plasma diluting channel (1-6).
7. The POCT immunodetection chip capable of performing multiple joint tests on whole blood at one time according to claim 6, wherein: the sample processing layer sealing plate (2) is provided with sample quantitative waste liquid bin air vents (2-4) communicated with the sample quantitative waste liquid bins (1-5).
8. The POCT immunodetection chip capable of performing multiple joint tests on whole blood at one time according to claim 1, wherein: divide liquid reaction module (9) including being discoid branch liquid reaction layer (6), divide and be equipped with a plurality of reaction detection room in liquid reaction layer (6), divide liquid reaction layer (6) to be equipped with and be used for the encapsulation reaction to detect branch liquid reaction layer shrouding (3) of room.
9. The POCT immunodetection chip capable of performing multiple joint tests on whole blood at one time according to claim 8, wherein: the reaction detection chamber is internally provided with a sample quantitative bin (6-2), a blending reaction bin (6-4), a test strip placing groove (6-7) and a water-absorbing paper placing groove (6-9) which are sequentially communicated, the sample quantitative bin (6-2) is provided with a sample quantitative bin inlet (6-1) communicated with a sample processing module (8), a reaction reagent (7) is arranged in the blending reaction bin (6-4), a test strip (5) is arranged in the test strip placing groove (6-7), water-absorbing paper (4) is arranged in the water-absorbing paper placing groove (6-9), and a detection window (6-8) corresponding to the test strip placing groove (6-7) is arranged on the liquid separation reaction layer (6).
10. The POCT immunodetection chip capable of performing multiple joint tests on whole blood at one time according to claim 9, wherein: the liquid separation reaction layer (6) is provided with uniform mixing reaction bin air holes (6-5) corresponding to the uniform mixing reaction bins (6-4).
11. The POCT immunodetection chip capable of performing multiple joint tests on whole blood at one time according to claim 10, wherein: the sample quantitative bin (6-2) is communicated with the uniform mixing reaction bin (6-4) through a reaction siphon channel (6-3).
12. The POCT immunodetection chip capable of performing multiple joint tests on whole blood at one time according to claim 11, wherein: the blending reaction bin (6-4) is communicated with the test strip placing groove (6-7) through a capillary capturing channel (6-6).
13. The POCT immunodetection chip capable of performing multiple joint tests on whole blood at one time according to claim 8, wherein: and a plasma blending diluent conveying hole (3-1) is formed in the liquid separation reaction layer sealing plate (3), and the plasma blending diluent in the sample processing module (8) enters a reaction detection chamber of the liquid separation reaction module (9) through the plasma blending diluent conveying hole (3-1).
14. The POCT immunodetection chip capable of performing multiple joint tests on whole blood at one time according to claim 13, wherein: a sealing plate sample adding yielding hole (3-2) is arranged on the liquid separating reaction layer sealing plate (3).
15. The POCT immunodetection chip capable of performing multiple joint tests on whole blood at one time according to claim 14, wherein: sample loading yielding holes (6-11) corresponding to the sample loading buffer bins (2-6) and diluent loading yielding holes (6-12) corresponding to the diluent loading buffer bins (2-7) are arranged on the liquid separation reaction layer (6).
16. The POCT immunodetection chip capable of performing multiple joint tests on whole blood at one time according to claim 8, wherein: the liquid separation reaction layer sealing plate (3) is a thick base material single-sided adhesive, a thick base material double-sided adhesive or a plastic plate adhered with the double-sided adhesive.
17. The POCT immunodetection chip capable of performing multiple joint tests on whole blood at one time according to claim 16, wherein: when the liquid separation reaction layer sealing plate (3) is a plastic plate adhered with double-sided adhesive, the plastic plate is fixed with the liquid separation reaction layer (6) through ultrasonic welding or laser welding.
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| CN216285330U (en) * | 2021-10-27 | 2022-04-12 | 苏州含光微纳科技有限公司 | Biochemical project detection device |
| CN114113642A (en) * | 2021-11-15 | 2022-03-01 | 成都微康生物科技有限公司 | Detection kit and method for performing coagulation analysis by using microfluidic technology |
| CN114558629A (en) * | 2022-03-03 | 2022-05-31 | 四川微康朴澜医疗科技有限责任公司 | Microfluidic thrombus elasticity analysis and detection kit |
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Denomination of invention: POCT immunoassay chip capable of single whole blood sampling and multiple joint tests Effective date of registration: 20231227 Granted publication date: 20210611 Pledgee: Zhejiang Mintai Commercial Bank Co.,Ltd. Sichuan Tianfu New Area Sub branch Pledgor: CHENGDU WEIKANG BIOTECHNOLOGY CO.,LTD.|Sichuan Weikang Park LAN Medical Technology Co.,Ltd. Registration number: Y2023980074782 |
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