CN108489764A - A kind of centrifugal quantitative collection CD - Google Patents
A kind of centrifugal quantitative collection CD Download PDFInfo
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- CN108489764A CN108489764A CN201810449309.7A CN201810449309A CN108489764A CN 108489764 A CN108489764 A CN 108489764A CN 201810449309 A CN201810449309 A CN 201810449309A CN 108489764 A CN108489764 A CN 108489764A
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- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 79
- 210000004369 blood Anatomy 0.000 abstract description 13
- 239000008280 blood Substances 0.000 abstract description 13
- 238000001514 detection method Methods 0.000 abstract description 10
- 238000012360 testing method Methods 0.000 abstract description 9
- 239000007787 solid Substances 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 7
- 210000002798 bone marrow cell Anatomy 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 208000014951 hematologic disease Diseases 0.000 description 2
- 208000019838 Blood disease Diseases 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 210000001772 blood platelet Anatomy 0.000 description 1
- 238000009534 blood test Methods 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000000093 cytochemical effect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007723 die pressing method Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 238000005530 etching Methods 0.000 description 1
- 208000018706 hematopoietic system disease Diseases 0.000 description 1
- 208000007475 hemolytic anemia Diseases 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 230000003907 kidney function Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 208000019423 liver disease Diseases 0.000 description 1
- 230000003908 liver function Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Hydrology & Water Resources (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention discloses a kind of centrifugal quantitative collection CD, including CD, the reagent being located on CD, the quantitative pond being located on CD, the run-off and diversion pipe that are located on CD.Quantitative pond is connect with run-off, and one end of diversion pipe is connect with reagent, and the other end is connect with quantitative pond.Diversion pipe include conduit A, with the conduit A U-tubes connecting and the conduit B being connect with U-tube.The free end of conduit A is connect with reagent, and the free end of conduit B is connect with the input terminal in quantitative pond.The present invention will be centrifuged and is combined with quantitative collection, can realize the reagent quantitative acquisition in blood testing field, picking rate is fast, and levels of precision is high, can provide solid accuracy guarantee to subsequent detection work.
Description
Technical field
The present invention relates to blood testing fields, and in particular to a kind of centrifugal quantitative collection CD.
Background technology
Clinical blood detection can be divided into blood generally detect, the test in laboratory of hemolytic anemia, bone marrow cell detect,
Bracket for blood grouping and cross match blood test.It can detect that the hematology of common hematological diseases holds sign.
One of the most common is that blood generally detects（That is blood routine）.I.e. to red blood cell, leucocyte and blood platelet these three
The amount and matter of system are detected and analyze.It is also wanted with the relevant detection of various diseases if blood disease is in addition to blood routine to be detected
Carry out bone marrow cell detection, blood cyto-chemical stain analysis etc.；It is main for the blood testing project of hepatopathy and kidney patient
Detection etc. including liver function, renal function；And for some communicable diseases, the detection of antibodies in blood is the weight of diagnosis
It will foundation.
Traditional detection means is detected for some index or certain specific substance after sample collection, is examined
Ranging sequence is more, and the detection of a routine needs more more equipment operations of people, and detection time is long, and testing cost is high, is its outstanding feature.
Especially it is certain need to be detected using reagent in the case of, a variety of interference such as sample error, instrumental error, human error
Factor can all influence testing result.Although the spreading speed of automatic detection system is obviously accelerated in recent years, easily tens
Ten thousand purchase costs even up to a million, general hospital and testing agency are difficult to bear.Especially blood testing parameter is various, inspection
Measurement equipment inevitably has specific aim again, also exacerbates this trend from another point of view.
Invention content
The present invention is intended to provide a kind of centrifugal quantitative collection CD that can carry out quantitative collection to reagent.
The present invention adopts the following technical scheme that：
A kind of centrifugal quantitative collection CD, including CD, be located on CD reagent, be located on CD quantitative pond, set
Run-off on CD and diversion pipe；
The quantitative pond is connect with run-off；
The run-off is located at quantitative pond close to the side of cd centre；
The reagent is located at quantitative pond close to the side of cd centre；
One end of the diversion pipe is connect with reagent, and the other end is connect with quantitative pond.
As further solution：The diversion pipe include conduit A, with the conduit A U-tubes connecting and and U-tube
The conduit B of connection；
The free end of the conduit A is connect with reagent；
The free end of the conduit B is connect with the input terminal in quantitative pond；
The minimum diameter of the conduit A is more than the maximum gauge of U-tube；
The minimum diameter of the conduit A is more than the maximum gauge of conduit B.
As further solution：Further include cache pool and the conduit C being connect with cache pool；
The cache pool is located at quantitative pond close to the side of cd centre；
The free end of the conduit C is connect with the input terminal in quantitative pond.
As further solution：The run-off includes the pond A and pond B being connect with pond A；
The pond B is located at sides of the pond A far from cd centre；
The quantitative pond is connect with pond A；
The quantitative pond and the junction of pond A are located on sides of the pond A far from cd centre.
As further solution：It is additionally provided with surge pipe on the cache pool；
One end of the surge pipe is connect with cache pool, and the other end is open end.
As further solution：It is additionally provided with delivery line on the cache pool.
As further solution：Minimum range between the conduit C and cd centre is less than reagent and light
Minimum range between disk center.
As further solution：The reagent is equipped with barometric pipe；The freedom of the barometric pipe
End is open end.
The good effect that the present invention generates is as follows：
The principle that the present invention is combined using centrifugation and quantitative collection can carry out quantitative collection to reagent.Reagent is filled into
In reagent or reagent is directly built in CD when making CD.When CD rotates, effect of the reagent in centrifugal force
Under be transferred in quantitative pond, extra part is transferred in run-off.After the completion of transfer, CD rotates backward, in quantitative pond
Reagent is flowed out along conduit C.Amount of reagent is determined by quantitative pool volume, is avoided instrumental error when manually quantifying, human error, is regarded
The interference of many factors such as line error can effectively improve quantitative accuracy.
The run-off of the present invention uses catamaran design, and when CD rotates forward, the reagent shifted from quantitative pond enters pond
A, under the influence of centrifugal force, reagent are eventually transferred into the B of pond.When CD rotates backward, the reagent in the B of pond has far from CD
The movement tendency at center, but pond B is not connect with quantitative pond, therefore when reagent outflow in quantitative pond, the reagent in the B of pond is kept
It is motionless, it can ensure that reagent collection capacity is equal with quantitative pool volume, to ensure acquisition precision.
When CD rotates backward, the hydraulic pressure in conduit A is higher than the hydraulic pressure in U-tube and conduit B.Reagent in quantitative pond exists
During outflow, it can only be flowed out from conduit C.Anti-return design can equally improve acquisition precision.
Description of the drawings
Fig. 1 is the structural diagram of the present invention；
Wherein：11 reagents, 12 quantitative ponds, 13 pond A, 14 pond B, 21 conduit A, 22U type pipes, 23 conduit B, 3 cache pools, 4 conduits
C, 5 surge pipes, 6 delivery lines, 7 barometric pipes.
Specific implementation mode
With reference to Fig. 1, invention is further explained.
The present invention adopts the following technical scheme that：
A kind of centrifugal quantitative collection CD, including CD, the reagent 11 being located on CD, the quantitative pond that is located on CD
12, the run-off and diversion pipe being located on CD；
The quantitative pond 12 is connect with run-off；
The run-off is located at quantitative pond 12 close to the side of cd centre；
The reagent 11 is located at quantitative pond 12 close to the side of cd centre；
One end of the diversion pipe is connect with reagent 11, and the other end is connect with quantitative pond 12.
As further solution：The diversion pipe include conduit A21, the U-tube being connect with conduit A21 22 and with
The conduit B23 that U-tube 22 connects；
The free end of the conduit A21 is connect with reagent 11；
The free end of the conduit B23 is connect with the input terminal in quantitative pond 12；
The minimum diameter of the conduit A21 is more than the maximum gauge of U-tube 22；
The minimum diameter of the conduit A21 is more than the maximum gauge of conduit B23.
As further solution：Further include cache pool 3 and the conduit C4 being connect with cache pool 3；
The cache pool 3 is located at quantitative pond 12 close to the side of cd centre；
The free end of the conduit C4 is connect with the input terminal in quantitative pond 12.
As further solution：The run-off includes the pond A13 and pond B14 being connect with pond A13；
The pond B14 is located at sides of the pond A13 far from cd centre；
The quantitative pond 12 is connect with pond A13；
The junction of the quantitative pond 12 and pond A13 is located on sides of the pond A13 far from cd centre.
As further solution：Surge pipe 5 is additionally provided on the cache pool 3；
One end of the surge pipe 5 is connect with cache pool 3, and the other end is open end.
As further solution：Delivery line 6 is additionally provided on the cache pool 3.
As further solution：Minimum range between the conduit C4 and cd centre be less than reagent 11 with
Minimum range between cd centre.
As further solution：The reagent 11 is equipped with barometric pipe 7；The barometric pipe 7
Free end is open end.
With reference to the actual course of work, invention is further explained.
The present invention needs to coordinate dedicated centrifugally operated equipment, each operation that CD is store in the equipment required
Rotation time and velocity of rotation.The centrifugally operated equipment repeats no more not within the protection domain of the application.
CD mentioned in the present invention is made of high molecule plastic, and reagent 11 and quantitative pond 12 etc. are using molding system
Make, conduit A21, U-tube 22 and conduit B23 etc. are made of accurate die pressing or etching, are integrated on a CD.
In use, reagent is injected reagent 11 or directly uses the CD of built-in reagent packet.
Start centrifugally operated equipment, CD starts to rotate, and reagent is flow to along conduit A21, U-tube 22 and conduit B23
In quantitative pond 12, with the inflow of reagent, the air in quantitative pond 12 starts to shift into run-off.When quantitative pond 12 is filled
Afterwards, extra reagent flows in run-off.The air in run-off is compressed simultaneously, when the air pressure in run-off increases to centainly
After degree, reagent does not enter back into.
What the input terminal of conduit B23, conduit C4 and reagent 11 were connected together, the air pressure in conduit C4 is more than conduit
The air pressure of the input end of fluid pressure and reagent 11 in B23, therefore during CD rotates forward, reagent is to quantitative pond
12 flow rather than flow in conduit C4.
Description below is made to the pressure at this：
Conduit B23 and the pressure of quantitative 12 junction of pond are provided by the reagent in conduit A21 and U-tube 22, conduit C4 with it is quantitative
The pressure of 12 junction of pond is provided by the air in conduit C4.Under the influence of centrifugal force, the liquid in conduit A21 and U-tube 22
Body has the movement tendency far from cd centre, and the air in conduit C4 equally also has the movement tendency far from cd centre.
With reference to pressure formula P=ρ gh of reference fluids, as the pressure P that the air in conduit C4 provides1=ρ1g1h1More than conduit A21 and U
The pressure P that reagent in type pipe 22 provides2=ρ2g2h2When, it will be able to meet above-mentioned requirements.
After reagent fills up quantitative pond 12, continue to flow to pond A13.The reagent flowing in the A13 of pond still has separate
The movement tendency of cd centre can be flow to from pond A13 in the B14 of pond.Pond B14 in addition to other than the junction of pond A13, it is equal with face
For sealing surface, reagent can not escape after entering pond B14.
Then CD rotates backward, and reagent starts the outflow out of reagent 11, is flow in cache pool 3 by conduit C4.
Description below is gone out for the action at this：
When CD rotates backward, the reagent outflow out of quantitative pond 12 under the influence of centrifugal force, while the reagent in the B14 of pond is hindered
Gear, can not flow in quantitative pond 12.Reagent has the movement tendency opposite with CD rotation direction, and conduit C4 has just position at this time
Rear in quantitative pond 12, reagent start to flow to conduit C4.
At this point, still remain a large amount of reagents in conduit A21, U-tube 22 and conduit B23, the pressure of reagent offer, quantitative pond
The pressure that air provides in the centrifugal force and pond A13 that reagent is subject in 12, three's collective effect push the reagent in quantitative pond 12
It is flowed to conduit C4.Reagent is flowed ultimately to after conduit C4 in cache pool 3.
Meanwhile with the junction of U-tube 22 and conduit B23 compared with 22 junction of U-tube, the distance apart from cd centre
It is farther, therefore the pressure bigger of conduit A21 and the junction of U-tube 22.The diameter of especially conduit A21 is far longer than U-tube
The diameter of 22 and conduit B23, have also been formed simultaneously fluid-tight in conduit A21, and the two collective effect prevents reagent from flowing backwards.
Because needing to add different reagents when blood testing, different reagents needs to be added between timesharing, therefore especially increases
Cache pool 3 is added, the time that reagent enters reaction tank can be extended, to realize that the difference of time is added in different reagents.
The effect of surge pipe 5 is to be discharged to air extra in cache pool 3 when reagent flows into cache pool 3 on cache pool 3
It in air, avoids generating back pressure, reagent inflow is made to be obstructed.The effect of delivery line 6 is Connection Cache pond 3 and final reaction tank,
It introduces an agent into reaction tank.
In order to ensure air pressure balance, barometric pipe 7 is also especially increased in reagent 11.Because with the stream of reagent
Go out, the pressure reduction in reagent 11, external atmosphere pressure can be less than, the outflow of reagent is obstructed.The effect of barometric pipe 7 be by
Outside air is imported into reagent 11, makes up the pressure loss caused by reagent outflow.
Finally it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations, although
Present invention has been described in detail with reference to the aforementioned embodiments, it will be understood by those of ordinary skill in the art that, still may be used
To modify to the technical solution recorded in previous embodiment or equivalent replacement of some of the technical features, and
These modifications or replacements, the spirit and model of technical solution of the embodiment of the present invention that it does not separate the essence of the corresponding technical solution
It encloses.
Claims (8)
1. a kind of centrifugal quantitative collection CD, it is characterised in that：Including CD, the reagent being located on CD（11）, be located at
Quantitative pond on CD（12）, the run-off and diversion pipe that are located on CD；
The quantitative pond（12）It is connect with run-off；
The run-off is located at quantitative pond（12）Close to the side of cd centre；
The reagent（11）It is located at quantitative pond（12）Close to the side of cd centre；
One end of the diversion pipe and reagent（11）Connection, the other end and quantitative pond（12）Connection.
2. a kind of centrifugal quantitative collection CD according to claim 1, it is characterised in that：The diversion pipe includes conduit
A（21）And conduit A（21）The U-tube of connection（22）With with U-tube（22）The conduit B of connection（23）；
The conduit A（21）Free end and reagent（11）Connection；
The conduit B（23）Free end and quantitative pond（12）Input terminal connection；
The conduit A（21）Minimum diameter be more than U-tube（22）Maximum gauge；
The conduit A（21）Minimum diameter be more than conduit B（23）Maximum gauge.
3. a kind of centrifugal quantitative collection CD according to claim 1, it is characterised in that：It further include cache pool（3）With
With cache pool（3）The conduit C of connection（4）；
The cache pool（3）Positioned at quantitative pond（12）Close to the side of cd centre；
The conduit C（4）Free end and quantitative pond（12）Input terminal connection.
4. a kind of centrifugal quantitative collection CD according to claim 1, it is characterised in that：The run-off includes pond A
（13）With with pond A（13）The pond B of connection（14）；
The pond B（14）Positioned at pond A（13）Side far from cd centre；
The quantitative pond（12）With pond A（13）Connection；
The quantitative pond（12）With pond A（13）Junction be located at pond A（13）On side far from cd centre.
5. a kind of centrifugal quantitative collection CD according to claim 3, it is characterised in that：The cache pool（3）It is upper to go back
Equipped with surge pipe（5）；
The surge pipe（5）One end and cache pool（3）Connection, the other end is open end.
6. a kind of centrifugal quantitative collection CD according to claim 3, it is characterised in that：The cache pool（3）It is upper to go back
Equipped with delivery line（6）.
7. a kind of centrifugal quantitative collection CD according to claim 3, it is characterised in that：The conduit C（4）With CD
Minimum range between center is less than reagent（11）Minimum range between cd centre.
8. a kind of centrifugal quantitative collection CD according to claim 1, it is characterised in that：The reagent（11）On set
There is barometric pipe（7）；The barometric pipe（7）Free end be open end.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810449309.7A CN108489764B (en) | 2018-05-11 | 2018-05-11 | Centrifugal quantitative acquisition optical disk |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810449309.7A CN108489764B (en) | 2018-05-11 | 2018-05-11 | Centrifugal quantitative acquisition optical disk |
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| Publication Number | Publication Date |
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| CN108489764A true CN108489764A (en) | 2018-09-04 |
| CN108489764B CN108489764B (en) | 2024-06-14 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810449309.7A Active CN108489764B (en) | 2018-05-11 | 2018-05-11 | Centrifugal quantitative acquisition optical disk |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109211860A (en) * | 2018-09-29 | 2019-01-15 | 厦门大学 | A kind of multinomial detection CD and detection method |
| CN109283174A (en) * | 2018-09-29 | 2019-01-29 | 厦门大学 | A kind of quantitative detection CD and detection method |
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| CN104888873A (en) * | 2015-05-08 | 2015-09-09 | 沈阳航空航天大学 | Centrifugal type micro fluidic chip having backward pumping function |
| CN105353159A (en) * | 2014-08-20 | 2016-02-24 | 余波 | Microfluidic test device and method for operating the same |
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| CN208270244U (en) * | 2018-05-11 | 2018-12-21 | 石家庄禾柏生物技术股份有限公司 | A kind of centrifugal quantitative collection CD |
-
2018
- 2018-05-11 CN CN201810449309.7A patent/CN108489764B/en active Active
Patent Citations (7)
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|---|---|---|---|---|
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| CN105353159A (en) * | 2014-08-20 | 2016-02-24 | 余波 | Microfluidic test device and method for operating the same |
| CN104888873A (en) * | 2015-05-08 | 2015-09-09 | 沈阳航空航天大学 | Centrifugal type micro fluidic chip having backward pumping function |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109211860A (en) * | 2018-09-29 | 2019-01-15 | 厦门大学 | A kind of multinomial detection CD and detection method |
| CN109283174A (en) * | 2018-09-29 | 2019-01-29 | 厦门大学 | A kind of quantitative detection CD and detection method |
| CN109211860B (en) * | 2018-09-29 | 2023-11-17 | 厦门大学 | Polynomial detection optical disc and detection method |
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| Publication number | Publication date |
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| CN108489764B (en) | 2024-06-14 |
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