JP2007232674A - Centrifugal separation device and centrifugal separation method - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a centrifugal separation device capable of separating completely a component different in a specific gravity in a sample liquid, and capable of collecting quantitatively the sample liquid required for analysis. <P>SOLUTION: A rotational speed of a disk is reduced stepwisely by a centrifugal separation means, using constitution that a relation between a length h1 from a liquid face of the sample liquid inserted into a sample liquid separation storage part to a turning curved part in the first flow passage connected to the sample liquid separation storage part, and a length h2 from the liquid face of the sample liquid inserted into the sample liquid separation storage part to a turning curved part in the second flow passage connected to the sample liquid separation storage part satisfies h1<h2. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a centrifuge device and a centrifuge method of a rotational analyzer for separating, quantitatively collecting and analyzing sample liquids containing components having different specific gravities.

  2. Description of the Related Art Conventionally, as a method for optically analyzing a biological fluid, a method for analyzing using a microdevice having a liquid flow path is known. Microdevices can control fluids using a rotational analyzer, and can measure samples, separate cytoplasmic materials, transfer and distribute separated fluids using centrifugal force. It is possible to perform biochemical analysis.

  As a method for measuring a sample using centrifugal force, as shown in FIG. 11, a central storage unit 111 for storing a liquid to be diluted before analysis from the center to the periphery, a measurement chamber 112 and an overflow chamber 113. And a mixing chamber 114 and a measurement cell 115, the measuring chamber 112 is arranged substantially parallel to the overflow chamber 113, and is provided on the wall of the measuring chamber facing the supply port 116 in addition to the supply port 116 and the overflow port 117. There is a rotational analysis device characterized in that it has an opening 118 provided, this opening is always open and has a cross section much smaller than the supply port 116 and the overflow port 117, and this configuration Thus, the filling of the measuring chamber 112 is performed at high speed, and the overflow is immediately removed. Liquid begins to flow out of this chamber as soon as the metering chamber 112 begins to fill with liquid. Therefore, since the ratio of the supply time to the outflow time from the outflow outlet as a function of the ratio of the inflow cross-sectional area to the outflow outlet cross-sectional area can be reduced as much as desired, accuracy is given to the measurement (Patent Document 1).

  As shown in FIG. 12, the large fluid chamber 121 is connected to the large fluid chamber 121, and the measurement chamber 122 is arranged radially outward with respect to the large fluid chamber 121, and is connected to the measurement chamber 122. A rotational analysis device having an overflow chamber 123, a receiving chamber 124 disposed radially outward with respect to the measuring chamber 122, and capillary connection means 125 for supplying liquid from the measuring chamber 122 to the receiving chamber 124. Yes, the capillary coupling means 125 includes a siphon 126 having a capillary structure, and the elbow bend of the siphon 126 is at substantially the same distance from the center of the rotational analysis device as the radial re-inward point of the metering chamber 122. So that the capillary force is smaller than the centrifugal force during rotation of the rotational analysis device, so that the liquid / air interface is the same axis as the rotational analysis device axis. Having a radius equal to the distance from the center of the rotational analysis device to the radial re-inward point of the metering chamber 122, the metering chamber 122 is filled with excess liquid Flows into the overflow chamber 123. When the rotation analysis device is stopped, the liquid filled in the measuring chamber 122 flows into the capillary connection means 125 by capillary force, and rotates again to start the siphon 126, and the liquid existing in the measuring chamber 122 is received. It is discharged into the chamber 124 (Patent Document 2).

Furthermore, as shown in FIG. 13, from the inner periphery toward the outer periphery, the outer peripheral side is provided with a storage part 131 and a blood cell storage part 132 formed in a fan shape, and a portion 133 connecting the blood cell storage part 132 and the storage part 131 is: It has a convex shape so that blood cell components that have flowed in by centrifugation do not flow back to the reservoir 131. Furthermore, a siphon-shaped output flow path 134 is connected to the side surface of the storage portion 131, and the sample liquid after the operation can be supplied from the output flow path 134 to the next operation region. The raw blood is supplied to the storage unit 131 via the supply channel 135, and a blood cell component having a high specific gravity is stored in the blood cell storage unit 132 by centrifugal force. The balance between the capillary force and the centrifugal force applied to the solution in the output flow path 135 connected to the reservoir 131 is reversed by lowering the number of revolutions in a state where the separation is almost completed, and remains in the reservoir 131 by centrifugation. Plasma / serum components are discharged to the next operation area via the output flow path 135. (Patent Document 3)
JP 61-167469 A Japanese National Patent Publication No. 5-508709 JP-A-2005-345160

  However, in the configuration of the prior art shown in FIG. 11 and FIG. 12, when the rotational analysis device is rotating, the centrifugal force is greater than the surface tension acting between the liquid and the wall surface of the measuring chamber. However, when the rotation is decelerated or stopped to move to the next process, the liquid is released from the centrifugal force and at the same time the interface between the liquid and the overflow wall. The surface tension started to work, and the surface tension caused the liquid to flow along the wall of the overflow port into the overflow chamber, so that precise measurement was not possible. In addition, since the amount of liquid flowing out varies depending on the physical property values of the liquid, it is necessary to change the size of the measuring chamber for each liquid to be analyzed.

  Furthermore, in the configuration of the prior art as shown in FIG. 13, the centrifugal separation based on the difference in specific gravity is possible. However, since the capillary tube is directly connected to the reservoir into which blood is introduced, the capillary tube before separation is used. The blood cells that had entered inside remained in the capillaries as they were, and there was a possibility that blood cells would be mixed into the serum / plasma to be collected.

  In various configurations including an operation area for the purpose of analysis, selective separation of a specimen and quantification of a necessary amount are indispensable elements. Various methods for quantifying specimens have been devised. In the case of blood analysis, blood used as a specimen has a large content component and is divided into blood cells and plasma or serum components. Of these, plasma or serum components are mostly used for measurement. Therefore, when the sample is selectively quantified, it is necessary to take two steps: a blood cell separation step and a plasma or serum quantification step.

In order to solve the above-mentioned conventional problems, the centrifugal device of the present invention is a centrifugal device for performing centrifugal separation by centrifugal force and capillary force generated by rotating a sample liquid to be analyzed.
A sample liquid reservoir for injecting and storing the sample liquid, and a difference in specific gravity of the sample liquid that is connected to the sample liquid reservoir and a flow path and is located radially outside the sample liquid reservoir. A first sample solution and a second sample solution which are centrifuged using a first sample solution and a sample solution separation and storage unit for separating and storing the first sample solution and a second sample solution connected to both radially outer ends of the sample solution separation and storage unit A first and a second fixed amount storage section for storing the first and second sample liquids through one flow path and a second flow path, respectively.
The length (h1) in the radial direction from the liquid surface of the sample liquid to be inserted into the sample liquid separation / storage part to the bent tube part of the first flow path connected to the sample liquid separation / storage part is the sample. It is characterized by being shorter than the length (h2) in the radial direction from the liquid surface of the sample liquid to be inserted into the liquid separation storage section to the bent portion of the second flow path connected to the sample liquid separation storage section. It is a thing.

  In addition, the centrifugation method of the present invention is the first rotation speed in the centrifugation method of the analysis sample solution in which the sample solution to be analyzed from the sample reservoir is rotated by centrifugal force and capillary force. The sample liquid in the sample liquid storage part is separated and stored according to the difference in specific gravity, and then decelerated to a second speed, and the first sample liquid having a light specific gravity is transferred from the sample liquid separation storage part to the quantitative storage part. The second sample liquid having a higher specific gravity is weighed and transferred to the quantitative storage section and then rotated step by step. The second sample liquid is then decelerated to a third rotational speed that is slower than the second rotational speed. It is characterized by reducing the speed and centrifuging.

  According to the centrifugal separation device and the centrifugal separation method of the present invention, the components having different specific gravity of the sample liquid can be completely separated by the rotational analysis means, and the sample liquid necessary for analysis can be collected quantitatively.

  Hereinafter, embodiments of the centrifugal separation device of the present invention will be described in detail with reference to the drawings.

  FIG. 1 is a schematic diagram showing the configuration of a centrifuge device in the first embodiment of the present invention. FIG. 2 is a diagram showing the panel 12 in which the microchannel 13 for the centrifuge device in the first embodiment is formed. FIG. 3 is a detailed view of the sample liquid separation and storage unit 22 that is the microchannel 13. FIG. 4 is a configuration diagram of the panel 12 in which the microchannel 13 is formed.

  The microchannel 13 formed on the panel 12 is manufactured by injection-molding a pattern of the uneven microchannel 13 as shown in FIG. 4 on the middle substrate 42, and injecting the sample liquid to be analyzed into the sample liquid storage section 21. In addition, by installing the rotary analyzer 11 in which a plurality of panels 22 can be arranged, it is possible to centrifuge and sample the sample liquid using centrifugal force and capillary force.

  As shown in FIG. 4, the panel 13 of the present embodiment is composed of an upper substrate 41, a middle substrate 42 on which microchannels 13 are formed by extraction, and a lower substrate 43. Each substrate is pressure-bonded with an adhesive. Yes. The thickness of each substrate is 1 to 3 mm in the first embodiment, but is not particularly limited. Regarding the shape of each substrate, a rounded square shape is used in the first embodiment, but other shapes such as a sector shape, a trapezoidal shape, a circular shape, and the like according to the purpose are possible. As the material of each substrate, a transparent polycarbonate-based resin was used from the viewpoint of easy moldability, easy analysis, mass production, and low cost.

  The microchannel 13 includes a sample liquid storage section and a flow path for transferring the sample liquid between the storage section and the storage section. The wall surface of the microchannel 13 is subjected to hydrophilic treatment in order to reduce viscous resistance and promote fluid movement. Here, the hydrophilic treatment mainly refers to a state where the contact angle between the sample and the wall surface is less than 90 degrees, and when the contact angle is 90 degrees or more, it is assumed that water repellent treatment is performed. In general, the lower the contact angle and the smaller the cross-sectional area of the capillary, the stronger the capillary force. In Example 1, surface treatment was performed on the wall surface of the microchannel 13 using a surfactant for hydrophilic treatment, and the contact angle was controlled to be about 70 degrees. Other surface treatment methods include a surface treatment method using an active gas such as plasma, corona, ozone, fluorine, or a surface treatment by embedding hydrophilic fine particles.

  Next, a specific configuration of the microchannel 13 in the first embodiment will be described. As shown in FIG. 2, the configuration of the microchannel 13 includes a sample reservoir 21 for injecting / retaining the sample liquid and a sample liquid separation / reservoir for performing centrifugation using the difference in specific gravity of the sample liquid. 22, a first quantitative storage unit 23 for storing the separated light sample with a specific gravity, and a second quantitative storage unit 24 for storing a sample with a high specific gravity.

  Further, as shown in FIG. 3, a separation wall 32 is formed on the radially outer wall surface of the sample liquid separation storage unit 22 from the approximate center of the wall surface toward the rotation center of the rotation analyzer. The separation wall 32 is a separation wall 32 for separating and storing the sample liquid separated due to the difference in specific gravity into the first separation storage part 22a and the second separation storage part 22b. That is, a sample liquid having a high specific gravity and a sample liquid having a low specific gravity are roughly separated into a first separation storage part 22 a and a second separation storage part 22 b by the separation wall 32 and stored in the sample liquid separation storage part 22. Is.

  Further, a backflow prevention valve 31 for preventing backflow is provided at the tip of the separation wall. The backflow prevention valve 31 may have any shape as long as it has a protruding portion extending in the direction opposite to the rotation direction at the radially inner end portion of the separation wall 32 as shown in FIG. Normally, in a state where the rotation speed is constant and acceleration is not applied, in the state where the sample liquid has all moved to the sample liquid separation and storage unit 22 (FIG. 3A), the liquid surface is localized at an approximately equal distance from the center of rotation. . However, when the rotational speed is decelerated, acceleration is applied in the direction opposite to the rotation direction. Therefore, an inertial force is generated in the sample liquid opposite to the acceleration, that is, in the rotation direction, and the vector of centrifugal force and inertial force is generated in the sample liquid. A force in the direction of synthesis is applied (FIG. 3B). Due to the influence, the sample liquid with a high specific gravity, which has been centrifuged in the second separation and storage section 22b, tries to enter the first separation and storage section 22a beyond the separation wall 32. At this time, by providing the backflow prevention valve 32 as shown in FIG. 3, it is possible to prevent the sample liquid having a heavy specific gravity from entering the first separation storage section 32 beyond the separation wall 32.

  In particular, when separating blood into plasma / serum and blood cells, the volume of the second separation reservoir 22b in which a sample liquid with a high specific gravity is stored is 50% to 60% of the sample liquid to be injected first. . This is because the ratio of blood cells 34 having a high specific gravity contained in human blood is generally 30% to 50%, and the capacity of the second separation and storage unit 22b for storing the blood cells 34 is 50% or more. This is because the sample liquid having a high specific gravity does not overflow to the first separation storage part 22a side where the sample liquid having a low specific gravity is stored. On the other hand, if the volume of the second separation reservoir 22b is excessively increased, it is necessary to increase the amount of blood 61 to be injected first in order to perform quantitative collection of the necessary amount of serum / plasma 33 as a sample. Or the volume of the second separation reservoir 22b may be 60% or less of the sample solution to be injected. Is desirable.

  The depth of each storage part is 2 mm, and the capacity | capacitance of 15 microliters or more is ensured. Furthermore, air holes 25 are provided in the upper substrate 41 in each storage unit in order to facilitate transport of the sample. The air holes 25 are formed so that the inner walls of the air holes are water repellent so that the sample does not scatter outside the panel 12, and the diameter of the air holes is 1 mm or less.

  Each storage part and the storage part are connected by a flow path. Specifically, the first flow path 26 that connects the radially outer wall surface of the sample liquid storage portion 21 to the radially inner wall surface of the sample liquid separation storage portion 22 and the radial direction of the first separation storage portion 22a. The second flow path 27 that connects the outer side, the side wall surface positioned in the rotation direction of the first quantitative reservoir 23, the radially outer side of the second separation reservoir 22 b, and the second quantitative reservoir 24 It is the 3rd flow path 28 which connects the side wall surface located in the direction opposite to a rotation direction.

  In particular, the second flow path 27 and the third flow path 28 have a siphon shape including a bent pipe portion 29 for turning back in the direction of the rotation center of the rotation analyzer 11. For the second flow path 27 and the third flow path 28, the distances from the liquid surface to the folded bent pipe section 29 when the sample liquid has entered the sample liquid separation and storage section 22 are H1 and H2, respectively. Sometimes it is formed to satisfy H1> H2. By using such a configuration, it is possible to selectively transfer the separated sample liquid to the first quantitative reservoir 23 and the second quantitative reservoir 24 by controlling the rotational speed of the rotation analyzer 11. it can. In Example 1, the width of the flow path was 0.6 to 1 mm and the depth was 0.2 mm.

  Next, the sample transfer process in the first embodiment will be described by taking blood centrifugation and quantitative collection as an example. The blood 61 can be classified mainly into serum / plasma 33 and blood cells 34, and blood cells have a higher specific gravity than serum / plasma 33.

  FIG. 5 shows a process flow of the first embodiment. FIG. 6 shows a schematic diagram of the flow of centrifugal separation and quantitative collection of serum / plasma 33 and blood cells 34 of blood 61. First, a necessary amount of blood 61, which is a sample solution, is injected into the sample solution storage unit 21 (FIG. 6A). Next, the panel 12 is installed in the rotation analyzer 11 and rotated at the rotation speed A to perform centrifugation (FIG. 6B). This rotational speed A is assumed that a sample exists in the middle of the second flow path 27 and the third flow path 28 between the connection portion of each sample liquid separation and storage section 22 and each folded pipe section 29. In this case, the centrifugal force 62 is sufficiently higher than the capillary force 63 applied to the sample solution. The rotation direction at this time is always the direction of the second flow path 27, that is, the counterclockwise direction in FIG. This direction of rotation is important and prevents blood cells 34 having a high specific gravity from being mixed into the first separation / storage part 22a side during centrifugation. By centrifuging the sample solution blood 61 at the rotation speed A, the sample solution blood 61 travels from the sample solution reservoir 22 through the first flow path 26 to the second separation reservoir 22b side from the heavier blood cell 61 having a higher specific gravity. It is stored temporarily. Needless to say, serum / plasma 33 having a light specific gravity is also mixed in the sample solution moving to the sample solution separation / storage unit 22 at this time. That is, in the case of the rotational speed A, the relationship of centrifugal force 62> capillary force 63 is established, and the sample solution moves to the sample solution separation / storage part 22 via the flow path 26.

  The blood 61 that has moved to the sample liquid separation and storage unit 22 is moved to the outer peripheral side by the centrifugal force 62, and the serum / plasma 33 having a lower specific gravity is driven to the inner peripheral side. Further, when centrifugation is performed, the serum / plasma 33 having a low specific gravity moves to the first separation / storage part side beyond the separation wall 32 formed in the sample liquid separation / storage part 22. At this time, the blood 61 moving from the sample solution storage unit 21 to the sample solution separation and storage unit 22 includes blood cells 34 having a high specific gravity. Since it enters into the 2nd separation storage part side, it does not enter into blood cell 34 with heavy specific gravity in the 1st separation storage part 22a side.

  When the blood 81 of the sample liquid is sufficiently separated into the first separation / storage section 22a and the second separation / storage section 22b, the speed is reduced from the rotation speed A to the rotation speed B (FIG. 6C). This rotational speed B is the rotational speed at which the balance between the capillary force 63 and the centrifugal force 62 applied to the serum / plasma 33 inside the second flow path 27 is reversed. At this time, the serum / plasma 33 having a low specific gravity separated by the centrifugal separation starts to move from the first separation reservoir 22 a to the first quantitative reservoir 23 through the second flow path 27. Serum / plasma 33 moving to the first fixed reservoir 23 is quantified at the time when all of the serum / plasma 33 enters the sample liquid separation reservoir 22, and is determined by the capacity of the second separation reservoir 22b. For example, when the volume of the second separation reservoir 22b is approximately 50% of the blood to be injected, the remaining approximately 50% is the amount of serum / plasma 33 having a low specific gravity. After the serum / plasma 33 having a light specific gravity is quantified in the first quantitative reservoir 24, it is decelerated from the rotational speed B to the rotational speed C (FIG. 6 (d)). This rotational speed C is the rotational speed at which the balance between the capillary force 63 and the centrifugal force 62 applied to the blood cells 34 inside the second flow path 28 is reversed. At this time, blood cells 34 having a high specific gravity remaining in the second separation and storage unit 22 b move to the second quantitative storage unit 24. All the separated blood 61 moves to the first quantitative reservoir 23 and the second quantitative reservoir 24 (FIG. 6 (e)), whereby quantitative collection of serum / plasma 33 with a low specific gravity as a specimen can be performed. Even if a process involving rotation occurs after this, blood cells 34 having a high specific gravity will not be mixed into the first quantitative reservoir 23.

  FIG. 7 is a schematic diagram showing the configuration of the centrifuge device in the second embodiment of the present invention. FIG. 8 is a view showing a panel 71 in which microchannels 72 for the centrifuge device in the second embodiment are formed. FIG. 9 is a detailed view of the sample liquid separation / storage part 81 of the microchannel 72.

  A difference from the configuration of the first embodiment is that a sample having a high specific gravity formed by the separation wall 82 is formed by separating the outer wall surface in the radial direction of the sample liquid separation storage unit 81 in a step shape as shown in FIG. The second separation and storage part 81a for storing the liquid and the separation and storage part 81b for storing the sample liquid with a low specific gravity constituted by the remaining regions are formed. Furthermore, the radially outer wall surface of the first separation and storage part 81a is formed so that the distance from the rotation center is closer to the backflow prevention valve 83 from the connection part of the first flow path 27. . In addition, the radially outer wall surface of the second separation storage portion 81b is formed so that the distance from the rotation center is closer to the separation wall 82 from the connection portion of the second flow path 28.

  By adopting such a configuration, it is possible to reduce the flow rate of the sample liquid when the sample liquid overflowing from the second separation storage section 81b moves to the first separation storage section 81a, and the second separation It becomes possible to suppress the backflow of the heavy component having accumulated in the reservoir 81b. Furthermore, by forming the slope as described above on the radially outer wall surface of each separation storage section, all the sample liquid stored in each separation storage section can be transferred into the flow path.

  FIG. 10 is a schematic diagram showing a sample transfer example taking blood centrifugation and quantitative sampling as an example. Blood 61, which is a sample solution, is injected into the sample solution reservoir 21 (FIG. 10A), and the rotation analyzer 11 is centrifuged at a rotation speed A (FIG. 10B). In the centrifugal separation at the first rotation speed A, the second sample liquid having a high specific gravity starts to be stored in the second separation storage unit 81b. That is, in the case of the rotational speed A, the relationship of centrifugal force 62> capillary force 63 is established, and the sample liquid moves to the separation storage part 81b through the first flow path 26.

  The blood 61 of the sample liquid moves from the blood cell 34 having a high specific gravity to the first separation storage part 81b of the sample liquid separation storage part 81 through the first flow path 26 from the sample liquid storage part 22 and is stored. . When the blood 61 of the sample liquid is sufficiently separated into the first separation / storage part 81a and the second separation / storage part 81b, the speed is reduced from the rotational speed A to the rotational speed B (FIG. 10 (c)). The serum / plasma 33 having a low specific gravity separated by the centrifugal separation starts to move from the first separation / storage unit 81b to the first quantitative storage unit 23 through the second flow path 27. After quantifying the serum / plasma 33 having a light specific gravity, the serum / plasma 33 is decelerated from the rotational speed B to the rotational speed C (FIG. 10 (d)). At this time, blood cells 34 having a high specific gravity remaining in the second separation and storage unit 81 b move to the second quantitative storage unit 24. Separation and quantitative collection of blood 61 are completed in a state where all the separated blood 61 is quantified in the first quantitative reservoir 23 and the second quantitative reservoir 24 (FIG. 10 (e)).

  The centrifugal separation device according to the present invention has a length (h1) from the liquid surface of the sample liquid to be inserted into the sample liquid separation / storage section to the bent tube section of the first flow path connected to the sample liquid separation / storage section. ) And the length (h2) from the liquid surface of the sample liquid to be inserted into the sample liquid separation / storage part to the bent pipe part of the second flow path connected to the sample liquid separation / storage part, By using a configuration satisfying h1 <h2, the rotational speed of the disk is reduced stepwise by the disk-type centrifugal separation means, so that components having different specific gravities in the sample liquid are completely separated, and the sample liquid required for analysis It is useful as a method that enables quantitative collection of

  The centrifuge device according to the present invention can also be applied to uses such as medical analysis test apparatuses that require separation and collection of blood cells, plasma, serum, and the like contained in blood components.

1 is a schematic configuration diagram of a centrifuge device in Embodiment 1 of the present invention. Detailed drawing of the panel mounted in the centrifuge device in Example 1 of the present invention Detailed view of the sample liquid separation storage part of the centrifugal separation device in Example 1 of the present invention The figure for demonstrating the structure of the panel of the centrifuge device in Example 1 of this invention. Process flowchart of centrifugal separation method in embodiment 1 of the present invention The figure for demonstrating centrifugation and quantitative collection of blood using the centrifuge device in Example 1 of this invention Schematic configuration diagram of a centrifuge device in Example 2 of the present invention Detail drawing of the panel mounted in the centrifuge device in Example 2 of the present invention Detailed view of the sample liquid separation storage part of the centrifuge device in Example 2 of the present invention The figure for demonstrating centrifugation and quantitative collection of the blood of the centrifuge device in Example 2 of this invention The figure for demonstrating the measurement of the sample of the conventional rotational analysis device The figure for demonstrating the measurement of the sample of the other conventional rotational analysis device The figure for demonstrating the measurement of the sample of other conventional rotational analysis devices

Explanation of symbols

DESCRIPTION OF SYMBOLS 11 Rotational analyzer 12 Panel of 1st Example 13 Microchannel of 1st Example 14 Center of rotation 21 Sample storage part 22 Sample liquid separation storage part 22a First separation storage part 22b Second separation storage part 23 First 1 Quantitative Reservoir 24 Second Quantitative Reservoir 25 Air Hole 26 First Flow Channel 27 Second Flow Channel 28 Third Flow Channel 29 Folded Tube Section 31 Backflow Prevention Valve 32 Separation Wall 33 Serum / Plasma 34 Blood cell 41 Upper substrate 42 Medium substrate 43 Lower substrate 61 Blood 62 Centrifugal force 63 Capillary force 71 Panel of the second embodiment 72 Microchannel of the second embodiment 81 Sample liquid separation and storage section 81a First separation and storage section 81b First 2 separation storage part 82 separation wall 83 backflow prevention valve 111 central accommodation part 112 measuring chamber 113 overflow chamber 114 mixing chamber 115 measurement cell 116 supply port 117 overflow port DESCRIPTION OF SYMBOLS 18 Opening 121 Large fluid chamber 122 Measuring chamber 123 Overflow chamber 124 Receiving chamber 125 Capillary connection means 126 Siphon 131 Storage part 132 Blood cell storage part 133 The part which connects a blood cell storage part and a storage part 134 Siphon-shaped output flow path 135 Supply flow Road

Claims (9)

In a centrifuge device for centrifuging by a centrifugal force generated by rotating a sample liquid to be analyzed and a capillary force,
A sample solution reservoir for injecting and containing the sample solution; and
A first sample liquid and a second sample liquid that are connected to the sample liquid storage section through a flow path and are located radially outward with respect to the sample liquid storage section and centrifuge the sample liquid using a difference in specific gravity of the sample liquid. A sample liquid separation and storage part that weighs and separates and stores the sample liquid,
First and second quantitative storages for storing the first and second sample liquids, respectively, via a first flow path and a second flow path connected to both radially outer ends of the sample liquid separation storage section. And
With
The length (h1) in the radial direction from the liquid surface of the sample liquid to be inserted into the sample liquid separation / storage part to the bent tube part of the first flow path connected to the sample liquid separation / storage part is the sample. It is characterized by being shorter than the length (h2) in the radial direction from the liquid surface of the sample liquid to be inserted into the liquid separation storage section to the bent portion of the second flow path connected to the sample liquid separation storage section. Centrifuge device. The sample solution separation and storage unit has a separation wall having a predetermined length toward the rotation center direction for separating and storing the sample solution, and is initially on the opposite side of the rotation direction separated by the separation wall. Approximately half the amount of the sample liquid stored in the sample storage part is weighed and stored in the storage part, and the sample liquid that exceeds it by subsequent rotation passes over the separation wall to the storage part on the rotation direction side. The centrifuge device according to claim 1, wherein the centrifuge device is stored. The centrifugal separation device according to claim 2, further comprising a protruding backflow prevention valve extending in a direction opposite to a rotation direction at a radially inner tip portion of the separation wall. The sample liquid separation and storage part is separated by forming a wall surface position on the outer side in the radial direction of the sample liquid separation and storage part in a step shape, and the sample liquid separation and storage part on the rotational direction side for measuring the first sample liquid The outer wall surface position in the radial direction is such that the volume of the sample liquid separating and storing portion that measures the second sample liquid from the outer wall surface position in the radial direction for measuring the second sample liquid is the sample liquid storage volume. The centrifuge device according to claim 1, wherein the centrifuge device is located on the rotation center side by a distance that should satisfy a volume of 50% to 60% of the sample liquid injected into the part. The position of the outer wall surface in the radial direction of the sample liquid separation and storage section on the rotational direction side for measuring the first sample liquid is from the center of rotation toward the sample liquid separation and storage section side for measuring the second sample liquid. The centrifuge device according to claim 1, wherein the centrifuge device is formed so as to be close to each other. 6. The centrifuge device according to claim 4, wherein the first sample solution is a serum / plasma component, and the second sample solution is a blood cell component. In the centrifugal separation method of the analytical sample liquid that is centrifuged by centrifugal force generated by rotating the sample liquid to be analyzed from the sample reservoir and capillary force,
The sample liquid in the sample liquid storage part is separated and stored by the difference in specific gravity at the first rotational speed,
Next, the second sample is decelerated to a second speed, and the first sample solution having a low specific gravity is weighed and transferred from the sample solution separation and storage unit to the quantitative storage unit.
Next, the second sample liquid having a heavy specific gravity is decelerated to a third rotational speed that is slower than the second rotational speed, and is metered and transferred to the quantitative reservoir.
A method for centrifuging an analysis sample solution, characterized by centrifuging at a reduced rotational speed stepwise. The sample solution separation and storage unit has a separation wall having a predetermined length toward the rotation center direction for separating and storing the analysis sample solution, and is opposite to the rotation direction separated by the separation wall at the beginning of rotation. Approximately half the amount of the sample liquid stored in the sample storage part is measured and stored in the storage part, and the sample liquid that exceeds it by the subsequent rotation passes the separation wall, and the storage part on the rotation direction side. The centrifugation method according to claim 7, wherein the centrifugal separation method is stored. 9. The method of centrifuging an analysis sample solution according to claim 8, wherein the first sample solution is a serum / plasma component, and the second sample solution is a blood cell component.

Images