CN109187945B - A kind of laying method of colloid gold test paper on CD - Google Patents
A kind of laying method of colloid gold test paper on CD Download PDFInfo
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- CN109187945B CN109187945B CN201811146762.7A CN201811146762A CN109187945B CN 109187945 B CN109187945 B CN 109187945B CN 201811146762 A CN201811146762 A CN 201811146762A CN 109187945 B CN109187945 B CN 109187945B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
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Abstract
The invention discloses a kind of laying method of colloid gold test paper on CD, including colloid gold test paper and CD, test paper is integrated to be placed on CD, including test paper layer and substrate, CD is equipped with channel, immersion liquid area and test paper placing groove, and channel connection external sample rest area and immersion liquid area, immersion liquid area are connected with test paper placing groove, test paper is placed in test paper placing groove, in the sample introduction end insertion immersion liquid area of test paper.A plurality of colloid gold test paper is integrated and is placed on CD by the present invention, it realizes and carries out repeatedly single item detection, the also various placement modes using test paper on CD on same CD, carry out the detection of disparity items, the detection accuracy of colloid gold test paper can be improved, improve detection efficiency.
Description
Technical field
The present invention relates to a kind of laying method of immunity inspection field more particularly to colloid gold test paper on CD.
Background technique
Colloidal gold is a kind of common labelling technique, is to be applied to the one of antigen-antibody using colloidal gold as tracer label object
The novel immunolabelling technique of kind has the advantages that its uniqueness.In recent years it is widely used in various biological studies.Make in clinic
Immunoblot assay nearly all uses its label.Simultaneously in streaming, Electronic Speculum, immune, molecular biology so that biochip
In all may example use.Colloid gold test paper diagnosis is developed using colloidal gold immunochromatographimethod technology, which is 90 years
The quick and easy immunology detection technology of one kind that generation just establishes on the basis of immunity infiltration technology.
Colloid gold test paper includes test paper layer and substrate, and test paper layer is thin film, is adhered on substrate, the solid-liquid of test paper layer
Reaction is the precision and accuracy that sample flow Uniform Flow just can guarantee detection in siphonage, detection process.Test paper layer is such as
Fruit touches other objects in addition to substrate, will form the gap between test paper layer and these objects, these gaps itself
Capillarity will cause sample liquids may generate bypass flowing without by test paper, this will affect inspection in these gaps
The accuracy of survey even makes detection fail.Therefore it needs to guarantee that the air pressure of test paper layer surface is uniform, cannot be touched on test paper layer
To anything, test paper needs to guarantee that test paper layer is natural reactiveness when placing.
Colloid gold test paper mainly uses flat manner when in use at present.It lays flat the plane for referring to test paper and ground is (horizontal
Face) in parallel, it is orthogonal with gravity direction, in this way convenient in test paper sample introduction one end dropwise addition sample.Colloid gold test paper has certain detection
Error, therefore colloid gold test paper is never applied in optical-disk type detection.
The present invention is that " IPS cell --- tumour cell is smart in vitro based on project of national nature science fund project " 31371444 "
The content of micro environment control chip lab new industrial research when closely knit " is unfolded.
Summary of the invention
The present invention provides a kind of laying method of colloid gold test paper on CD, is made in the detection of optical-disk type chip with realizing
With colloid gold test paper, the detection accuracy of test paper is improved.
Technical problem solved by the invention can be realized using following technical scheme:
A kind of laying method of colloid gold test paper on CD, it is characterized in that: including test paper and CD, the test paper is integrated
It is placed on CD, the test paper is colloid gold test paper, including test paper layer and substrate, and the CD is equipped with channel, immersion liquid area
With test paper placing groove, the channel connection external sample rest area and immersion liquid area, the immersion liquid area are connected with test paper placing groove, institute
It states test paper to be placed in test paper placing groove, in the sample introduction end insertion immersion liquid area of the test paper.
Preferably, a plurality of test paper can be integrated on the same CD.
Preferably, the bottom in the immersion liquid area is the minimum position of potential energy.
Preferably, the test paper is stood up in the test paper placing groove of CD, and length direction is parallel to cd side.
Preferably, the test paper bending is placed in test paper placing groove.
Preferably, when the test paper is bent including test paper layer, substrate is outside.
A plurality of colloid gold test paper is integrated and is placed on CD by the present invention, is realized and is carried out multiple individual event visual inspection on same CD
It surveys, also the various placement modes using test paper on CD, carries out the detection of disparity items, the inspection of colloid gold test paper can be improved
Precision is surveyed, detection efficiency is improved.
Detailed description of the invention
Fig. 1 is integrated schematic diagram of the colloid gold test paper on CD.
Fig. 2 is bend mode schematic diagram of the colloid gold test paper on CD.
Fig. 3 is other bend mode schematic diagrames of colloid gold test paper on CD.
In figure, 1- test paper, 2- CD, 11- test paper floor, 12- substrate, the channel 21-, 22- immersion liquid area, 23- test paper placing groove.
Specific embodiment
The present invention is described in further detail below with reference to embodiment, embodiments of the present invention are not limited thereto.
The total flow direction of liquid is extrorse from center in microchannel on centrifugal CD micro-fluidic chip.
In order to use colloid gold test paper in optical-disk type chip, colloid gold test paper needs the artificial gravity being suitable on CD
Direction, i.e. centrifugation driving force direction.Therefore, the length direction of colloid gold test paper needs parallel to the ground, or flat with cd side
Row.
On the other hand, since test paper needs to integrate in an optical disc, the length of colloid gold test paper is generally in 60mm or so, width
Generally in 3mm or so, length is bigger than the radius for generally detecting CD, thus colloid gold test paper need to be bent to a certain extent or
It folds, and the mode folded is limited, can only be bent in the longitudinal direction, and cannot be bent in the direction of the width, because reaction needs
Certain length is wanted, and to save colloid gold reagent as far as possible.Therefore the best folded orientation of colloid gold test paper is that width is vertical
In cd side, length is parallel to cd side, that is, is laterally located (or cry and stand up, as shown in Figure 1), that is, vertical in width
In the case of fold.If colloid gold test paper will lie on CD (situation i.e. parallel with CD in width), CD will have
Enough thickness, cost is higher, therefore best using standing up.
As shown in Figure 1, the present invention includes test paper 1 and CD 2, test paper 1 is integrated to be placed on CD 2, can on same CD 2
Integrate a plurality of test paper 1.Test paper 1 is colloid gold test paper, including test paper layer 11 and substrate 12.CD 2 is equipped with channel 21, immersion liquid area
22 and test paper placing groove 23, channel 21 connects sample deposition (not shown) and immersion liquid area 22, immersion liquid area 22 are put with test paper
It sets slot 23 to be connected, test paper 1 is stood up in the test paper placing groove 23 of CD 2, and length direction is parallel to cd side, the sample introduction of test paper 1
In the insertion immersion liquid area 22 of end, liquid is led into reaction zone by the siphonage of test paper 1.The bottom in immersion liquid area 22 is that potential energy is minimum
Position.
Test paper 1 needs to guarantee that test paper layer 11 is natural reactiveness when placing.It is discovered by experiment that when test paper 1 is bent
Siphonage is not influenced, as long as ensuring to be not exposed to any other impurity than air on test paper layer 11, therefore can will be tried
The bending of paper 1 is placed in test paper placing groove 23.The sensitivity and reliability that test paper 1 has certain bending to will not influence detection, examination
There are many bend modes of paper 1 (as shown in Figure 2,3), and the bend mode in Fig. 3 needs CD to have enough thickness, these bendings
Mode can be integrated in same CD 2, convenient for the more preferable same project and multiple detection projects of more accurately detecting in same light
It is integrated on disk 2.
The inner wall that test paper layer 11 is likely to be exposed in test paper placing groove 23 is arranged to laciniation, because of possible contact point
Shape is distributed in everywhere in the path of flowing, and can not form continuous gap leads to capillarity.This can ensure that in test paper
Sample flow not will receive the dynamic influence of by-pass, so that test paper placing groove 23 has support simultaneously and blocks the function of bypass flowing
Energy.
Using including test paper layer 11 when test paper 1 is bent, the bend mode of substrate 12 outside is curved because substrate 12 is rigid
Qu Shiyou outside elasticity, tension make the natural inner wall close to test paper placing groove 23 of substrate 12, form stationary state.It is this curved
The bending of test paper 1, which may be implemented, in bent mode does not influence to react.
The above is only present pre-ferred embodiments, is not intended to limit the scope of the present invention, therefore
Any subtle modifications, equivalent variations and modifications to the above embodiments according to the technical essence of the invention, still fall within
Protection scope of the present invention.
Claims (5)
1. laying method of a kind of colloid gold test paper on CD, it is characterized in that: including test paper and CD, the test paper is integrated to be put
Be placed on CD, the test paper is colloid gold test paper, including test paper layer and substrate, the CD be equipped with channel, immersion liquid area and
Test paper placing groove, the channel connection external sample rest area and immersion liquid area, the immersion liquid area is connected with test paper placing groove, described
Test paper is stood up in test paper placing groove, and length direction is parallel to cd side, and the sample introduction end of the test paper is inserted into immersion liquid area.
2. laying method of a kind of colloid gold test paper according to claim 1 on CD, it is characterized in that: the same light
A plurality of test paper can be integrated on disk.
3. laying method of a kind of colloid gold test paper according to claim 1 on CD, it is characterized in that: the immersion liquid area
Bottom be the minimum position of potential energy.
4. laying method of a kind of colloid gold test paper according to claim 1 on CD, it is characterized in that: the test paper is curved
Song is placed in test paper placing groove.
5. laying method of a kind of colloid gold test paper according to claim 4 on CD, it is characterized in that: the test paper is curved
When bent including test paper layer, substrate is outside.
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| CN201811146762.7A CN109187945B (en) | 2018-09-29 | 2018-09-29 | A kind of laying method of colloid gold test paper on CD |
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| CN201811146762.7A CN109187945B (en) | 2018-09-29 | 2018-09-29 | A kind of laying method of colloid gold test paper on CD |
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| CN109187945A CN109187945A (en) | 2019-01-11 |
| CN109187945B true CN109187945B (en) | 2019-07-12 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3816923A (en) * | 1973-04-12 | 1974-06-18 | Pee Dee Pathology Ass | Measuring apparatus |
| CN101261270A (en) * | 2007-03-09 | 2008-09-10 | 中国人民解放军军事医学科学院微生物流行病研究所 | Immunity-chromatography multiple detection test paper disk and immunity-chromatography multiple detection method |
| CN201555849U (en) * | 2009-11-18 | 2010-08-18 | 吉林大学第一医院 | Disc type collaurum immunochromatography joint detection plate |
| CN101949930A (en) * | 2010-09-13 | 2011-01-19 | 无锡市金坤生物工程有限公司 | Disc for fast detecting mycotoxin indexes |
| CN107727842A (en) * | 2017-11-13 | 2018-02-23 | 山东省环境保护科学研究设计院 | A kind of preparation and application of clenbuterol hydrochloride full constituent residual joint inspection test card |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE20010628U1 (en) * | 2000-06-14 | 2000-10-12 | Care Diagnostica Produktions U | Device for determining a substance |
| CN104792567B (en) * | 2015-04-30 | 2018-03-02 | 珠海市银科医学工程有限公司 | It is a kind of new to adopt just and tachysynthesis detection means and its operating method |
| CN107389549A (en) * | 2017-08-17 | 2017-11-24 | 武汉璟泓万方堂医药科技股份有限公司 | Rotating disc type collaurum/fluorescent test paper chip |
-
2018
- 2018-09-29 CN CN201811146762.7A patent/CN109187945B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3816923A (en) * | 1973-04-12 | 1974-06-18 | Pee Dee Pathology Ass | Measuring apparatus |
| CN101261270A (en) * | 2007-03-09 | 2008-09-10 | 中国人民解放军军事医学科学院微生物流行病研究所 | Immunity-chromatography multiple detection test paper disk and immunity-chromatography multiple detection method |
| CN201555849U (en) * | 2009-11-18 | 2010-08-18 | 吉林大学第一医院 | Disc type collaurum immunochromatography joint detection plate |
| CN101949930A (en) * | 2010-09-13 | 2011-01-19 | 无锡市金坤生物工程有限公司 | Disc for fast detecting mycotoxin indexes |
| CN107727842A (en) * | 2017-11-13 | 2018-02-23 | 山东省环境保护科学研究设计院 | A kind of preparation and application of clenbuterol hydrochloride full constituent residual joint inspection test card |
Non-Patent Citations (2)
| Title |
|---|
| Paper on a disc: balancing the capillary-driven flow with a centrifugal force;Hwang H et al.,;《Lab on a Chip》;20111021;第11卷(第20期);全文 |
| 基于离心力驱动微流控芯片的研究进展;张玲 等;《广州化工》;20101231;第38卷(第11期);全文 |
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| CN109187945A (en) | 2019-01-11 |
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