CN201984033U - SPR (surface plasmon resonance) detector for liquid phase microfluidic analysis system - Google Patents
SPR (surface plasmon resonance) detector for liquid phase microfluidic analysis system Download PDFInfo
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- CN201984033U CN201984033U CN2010206949309U CN201020694930U CN201984033U CN 201984033 U CN201984033 U CN 201984033U CN 2010206949309 U CN2010206949309 U CN 2010206949309U CN 201020694930 U CN201020694930 U CN 201020694930U CN 201984033 U CN201984033 U CN 201984033U
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Abstract
The utility model discloses an SPR (surface plasmon resonance) detector for a liquid phase microfluidic analysis system, which comprises an SPR sensor, a miniature flow cell, a signal processing circuit, a data acquisition circuit, a microcontroller internally provided with a USB (universal serial bus) communication interface and a circuit, wherein the miniature flow cell is jointed with the SPR sensor; the SPR sensor comprises an LED (light-emitting diode) light source, a parallel light alignment way, a polaroid sheet, a gathering light way, a cylindrical prism and a CCD (charge coupled device) detector; and a metal film is plated on the rectangular surface of the cylindrical prism. A biological sensitive film sensitive to a substance to be detected is adorned on the surface of the metal film of the detector so as to realize high sensitivity detection that whether a sample contains microcontent and trace substances, and the SPR detector for the liquid phase microfluidic analysis system has the functions that other detectors can not achieve. The SPR detector for the liquid phase microfluidic analysis system has the advantages of strong generality, high sensitivity, microsample, small size, low cost, and the like.
Description
Technical field
The utility model relates to the material analysis detection range, relates in particular to a kind of New-type detector that is applied to liquid phase microflow analysis system.
Background technology
SPR (English full name surface plasmon resonance) is the abbreviation of surface plasma body resonant vibration.The free electron that the evanescent wave that light produces when glass and the generation total reflection of metal interface place can cause the metal surface produces surface plasma (surface plasmon, SP), when the frequency of surface plasma and evanescent wave is identical with wave number, will produce surface plasma resonance.This phenomenon can make catoptrical intensity reduce suddenly, and very responsive to the refractive index of metallic film surface dielectric.Since nineteen eighty-three Liedberg etc. is used for the SPR technology after reacting to each other of IgG protein and its antigen measure, the SPR technology is few with its highly sensitive, required sample, sample need not mark and detection speed is fast etc., and characteristics are widely used in monitoring fields such as biochemistry, medical treatment, food, environment, become the focus of sensor research field.
Liquid phase microflow analysis system comprises that being applied to biochemical field solution composition analyzes various isolation technics, these isolation technics mainly contain capillary liquid chromatography, receive upgrade liquid chromatogram, Capillary Electrophoresis and micro-fluidic chip etc., and they are the most frequently used isolation technics of Pharmaceutical Analysis, environmental analysis, biochemical analysis and technical analysis.As the detecting device of liquid phase microflow analysis system " eyes ", be the vitals of analytical instrument, the quality of its performance directly has influence on performance index such as the detectability, sensitivity of analytical instrument.Detecting device commonly used at present, mainly contain electric conductivity detector, ampere detector, ultraviolet-visible detector, fluorescence detector and electrochemical detector etc. are though these detecting devices have high sensitivity, the advantage of low detectability, but their shortcomings all selective by force, the versatility difference.And the stronger differential refraction detector of versatility, then exist sensitivity low, problems such as the pond volume is bigger, and promptly required sample size is more generally can only be used for conventional Liquid Detection system such as common high performance liquid chromatography (flow is 1ml/min) or Flow Injection Analysis device.
The SPR detection technique is a kind of novel photoelectric detecting technology.Have characteristics such as detection sensitivity height, anti-electromagnetic interference performance is good, the sample consumption is few.Spr sensor is very responsive to its surperficial medium, the small variation (10 of the sensor surface fluid refracting rate of flowing through
-6～10
-8RIU), just can cause the variation of sensor output, its measuring process sample consumption is not more than 1ul.
Summary of the invention
The utility model provides a kind of highly versatile, highly sensitive, micro-sampling, the small-sized and inexpensive SPR detecting device that is applied to liquid phase microflow analysis system, this detecting device is based on surface plasma resonance (SPR) technology, by detecting the analysis of target solution refractive index realization to sample, combine differential refraction detector highly versatile and the high advantage of other detector sensitivity, by modification, can realize the specific detection of predetermined substance simultaneously to sensor surface.
A kind of SPR detecting device that is used for liquid phase microflow analysis system, comprise spr sensor, miniature flow cell, signal processing circuit, data acquisition circuit, internal USB communication interface circuit microcontroller and.Described miniature flow cell and spr sensor are fitted and are provided with, when solution to be measured by miniature flow cell water conservancy diversion during through spr sensor, spr sensor is converted into electric signal with the refractive index of solution to be measured, and by the electric signal realization amplification of signal processing circuit to spr sensor output, Filtering Processing, last microprocessor controls data acquisition circuit will be converted into digital signal with the corresponding electric signal of solution refractive index to be measured, and be uploaded to the computer terminal user program by built-in usb communication interface, carry out storing after the calculation process, and be shown in computer terminal user program interface.
Described spr sensor comprises led light source, directional light collimated light path, polaroid, converges light path, cylindrical prism and ccd detector, the square surface metal-plated membrane of cylindrical prism.The light beam that led light source sends, be directional light through directional light collimated light path collimation, and by polaroid extraction p polarized light, at last be converted into the interface place midline that wedge shape light multi-angle incides cylindrical prism and metal film via converging light path, the reflected light that produces incides ccd detector, and ccd detector output reflects the SPR spectral signal of the metallic film surface solution refractive index of flowing through.
Described metal film is golden film or gold and silver composite membrane.
Described miniature flow cell has air groove and U type groove by dimethyl silicone polymer (PDMS) material on the described miniature flow cell.Two interfaces of described U type groove are connected with kapillary respectively, form input channel and output channel, for measuring object solution provides the stream interface.Metallic film surface is close in the front of miniature flow cell, air in the air groove can be used as standard refraction rate correcting sensor, measurand solution is flowed into by input channel, and by U type groove water conservancy diversion through metallic film surface, thereby the SPR spectral signal of realizing the solution refractive index is measured, and is discharged by output channel at last.
SPR detecting device of the present utility model can be realized the highly sensitive detection of predetermined substance in the sample by modify the bio-sensitive film to different test substance sensitivities on metallic film surface, becomes the specific detection device.When having only a passage modified biological sensitive membrane, the another one passage is the modified biological sensitive membrane not, and then two passages can be respectively as measuring passage and reference channel, and realization reference mode is measured, to improve the measurement sensitivity of detecting device.
SPR detecting device of the present utility model is with Micrometer-Nanometer Processing Technology and the miniature flow cell of PDMS material, integrated technology such as highly sensitive spr sensor, high performance signal processing circuit, INTELLIGENCE DATA COLLECT TERMINAL and high-speed communication interface circuit, can realize the measurement of specificity mode and reference mode, have that versatility is good, highly sensitive, a micro-sampling, small-sized and cost performance advantages of higher.
Description of drawings
Fig. 1 is the structural representation block diagram of the utility model SPR detecting device;
Fig. 2 is the miniature flow cell of applying setting of the present utility model and the structural representation of spr sensor;
Fig. 3 is the structural representation of miniature flow cell of the present utility model;
Fig. 4 is that the A-A of miniature flow cell of the present utility model is to structural representation;
Fig. 5 is the synoptic diagram of the utility model SPR detecting device in liquid phase microflow analysis system applies.
Embodiment
As shown in Figure 1, a kind of SPR detecting device that is used for liquid phase microflow analysis system comprises spr sensor, miniature flow cell 11, signal processing circuit, puts microcontroller 1 (CY68013) and the computing machine and the user program of usb communication interface 2 circuit.Miniature flow cell 11 is fitted with spr sensor and is provided with, when solution to be measured by miniature flow cell water conservancy diversion during through spr sensor, spr sensor is converted into electric signal with the refractive index of solution to be measured, and by the electric signal amplification of signal processing circuit realization to spr sensor output, Filtering Processing, last microcontroller 1 will be converted into digital signal with the corresponding electric signal of solution refractive index to be measured by data acquisition circuit, and be uploaded to the computer terminal user program by built-in usb communication interface 2, carry out storing after the calculation process, and be shown in computer terminal user program interface.
As shown in Figure 2, spr sensor comprises led light source 3, directional light collimated light path 4, polaroid 5, converges light path 6, cylindrical prism 7 and ccd detector 8, cylindrical prism 7 square surface metal-plated membranes 9.The light beam that led light source 3 sends, be directional light through directional light collimated light path 4 collimations, and by polaroid 5 extraction p polarized lights, be converted into the interface place midline that wedge shape light multi-angle incides cylindrical prism 7 and metal film 9 via converging light path 6 at last, the reflected light that produces incides ccd detector 8, and ccd detector 8 outputs reflect the SPR spectral signal of the metal film 9 surface solution refractive indexes of flowing through.
As shown in Figure 3, Figure 4, miniature flow cell 11 is by the PDMS material, and miniature flow cell 11 is provided with air groove 15 and U type groove 10.Two interfaces of U type groove 10 are connected with kapillary respectively, form input channel 13 and output channel 14, for measuring object solution provides the stream interface.Metal film 9 surfaces are close in the front 16 of miniature flow cell 11, air in the air groove 15 can be used as standard refraction rate correcting sensor, measurand solution is flowed into by input channel 13, and by U type groove 10 water conservancy diversion through metal film 9 surfaces, thereby the SPR spectral signal of realizing the solution refractive index is measured, and is discharged by output channel 14 at last.The volume of U type groove 10 has determined the volume of flow cell, and miniature flow cell 11 can be used for protecting the metal film of spr sensor not scratched by hard thing simultaneously.
On metal film 9 surfaces, can modify different bio-sensitive films 12 according to the difference of measurand, can realize the highly sensitive detection of predetermined substance in the sample, become the specific detection device.When having only a passage modified biological sensitive membrane, the another one passage is the modified biological sensitive membrane not, and then two passages can be respectively as measuring passage and reference channel, and realization reference mode is measured, to improve the measurement sensitivity of detecting device.
As shown in Figure 5, during actual the use, solution to be measured is connected to the input channel 13 of the miniature flow cell 11 of SPR detecting device by kapillary, the biofilm surface of bio-modification (or through) flows out from output channel 14 flow of solution to be measured through metal film 9 surfaces.The variation of gold film surface solution component, the concentration change that specific adsorption causes are converted to spectral signal in real time by spr sensor, can realize detecting by the analysis to this signal.
User program by computer terminal can and be controlled the brightness of the led light source in the spr sensor 3 integral time of ccd detector 8; When sample was surperficial by miniature flow cell water conservancy diversion process spr sensor, the ccd detector 8 of spr sensor will be exported with the sample refractive index corresponding SPR spectral signal; This signal becomes digital signal corresponding by the microprocessor controls data acquisition circuit with this electrical signal conversion after the signal processing circuit amplification filtering; The computer terminal user program obtains the data of detecting device by the usb communication interface, carries out storage after the calculation process, is shown in computer terminal user program interface.User program is realized the check and analysis of object by the analysis to the SPR spectral signal.
Claims (4)
1. SPR detecting device that is used for liquid phase microflow analysis system, it is characterized in that: described SPR detecting device comprises the microcontroller of spr sensor, miniature flow cell, signal processing circuit, data acquisition circuit and internal USB communication interface;
Described spr sensor and miniature flow cell are fitted and are provided with; Described spr sensor is converted into electric signal with the refractive index of solution to be measured;
Described signal processing circuit connects spr sensor and data acquisition circuit, is used for the electric signal of spr sensor output is realized amplification, Filtering Processing;
Described data acquisition circuit connects signal processing circuit and microcontroller, is used for the electric signal after amplification, the Filtering Processing is converted into digital signal;
Described microcontroller internal USB communication interface is used for the control data Acquisition Circuit electric signal is converted into digital signal, and by built-in usb communication interface digital signal is uploaded to computing machine.
2. SPR detecting device as claimed in claim 1, it is characterized in that: described spr sensor comprises led light source, the directional light collimated light path, polaroid, converge light path, cylindrical prism, ccd detector, described cylindrical prism square surface metal-plated membrane, the light beam that led light source sends, be directional light through directional light collimated light path collimation, and by polaroid extraction p polarized light, at last be converted into the interface place midline that wedge shape light multi-angle incides cylindrical prism and metal film via converging light path, the reflected light that produces incides ccd detector, and ccd detector output reflects the SPR spectral signal of the metallic film surface solution refractive index of flowing through.
3. SPR detecting device as claimed in claim 1 is characterized in that: described miniature flow cell is provided with air groove and U type groove; Two interfaces of U type groove are connected with kapillary respectively, form input channel and output channel; Metallic film surface is close in the front of miniature flow cell.
4. SPR detecting device as claimed in claim 1 is characterized in that: be modified with bio-sensitive film on the metallic film surface of detecting device.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104237131A (en) * | 2014-10-11 | 2014-12-24 | 重庆医科大学 | Flow cell |
| CN104897611A (en) * | 2014-03-03 | 2015-09-09 | 黄辉 | Biochemical analyzer based on optofuidic sensor with inner cavity structure |
| CN105374265A (en) * | 2015-12-15 | 2016-03-02 | 徐州医学院 | High efficiency liquid chromatograph experiment teaching model and experiment method |
| CN106198400A (en) * | 2016-08-29 | 2016-12-07 | 苏州奥普特克自动化科技有限公司 | A kind of optoacoustic associating high flux real-time detecting system |
| CN106442425A (en) * | 2016-12-19 | 2017-02-22 | 杭州晶百检测技术有限公司 | SPR bio-sensing chip |
| CN106525777A (en) * | 2016-12-19 | 2017-03-22 | 杭州晶百检测技术有限公司 | Handheld SPR detector and bio-sample detection method |
| CN111474155A (en) * | 2020-04-28 | 2020-07-31 | 广东博创佳禾科技有限公司 | Ralstonia solanacearum solution guiding device |
| CN112098366A (en) * | 2020-07-27 | 2020-12-18 | 桂林电子科技大学 | Embedded double-U-shaped refractive index sensor for realizing three Fano resonances |
| CN113777079A (en) * | 2021-09-09 | 2021-12-10 | 北京化工大学 | Integrated surface plasma resonance chip and preparation method thereof |
-
2010
- 2010-12-31 CN CN2010206949309U patent/CN201984033U/en not_active Expired - Fee Related
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104897611A (en) * | 2014-03-03 | 2015-09-09 | 黄辉 | Biochemical analyzer based on optofuidic sensor with inner cavity structure |
| CN104237131A (en) * | 2014-10-11 | 2014-12-24 | 重庆医科大学 | Flow cell |
| CN105374265A (en) * | 2015-12-15 | 2016-03-02 | 徐州医学院 | High efficiency liquid chromatograph experiment teaching model and experiment method |
| CN105374265B (en) * | 2015-12-15 | 2018-05-22 | 徐州医学院 | A kind of high performance liquid chromatograph experimental teaching model of cobalt and experimental method |
| CN106198400A (en) * | 2016-08-29 | 2016-12-07 | 苏州奥普特克自动化科技有限公司 | A kind of optoacoustic associating high flux real-time detecting system |
| CN106442425A (en) * | 2016-12-19 | 2017-02-22 | 杭州晶百检测技术有限公司 | SPR bio-sensing chip |
| CN106525777A (en) * | 2016-12-19 | 2017-03-22 | 杭州晶百检测技术有限公司 | Handheld SPR detector and bio-sample detection method |
| CN111474155A (en) * | 2020-04-28 | 2020-07-31 | 广东博创佳禾科技有限公司 | Ralstonia solanacearum solution guiding device |
| CN112098366A (en) * | 2020-07-27 | 2020-12-18 | 桂林电子科技大学 | Embedded double-U-shaped refractive index sensor for realizing three Fano resonances |
| CN112098366B (en) * | 2020-07-27 | 2022-10-25 | 桂林电子科技大学 | Embedded double-U-shaped refractive index sensor for realizing three Fano resonances |
| CN113777079A (en) * | 2021-09-09 | 2021-12-10 | 北京化工大学 | Integrated surface plasma resonance chip and preparation method thereof |
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