CN104614345A - Portable optical fiber SPR (Surface Plasma Resonance) image sensor - Google Patents
Portable optical fiber SPR (Surface Plasma Resonance) image sensor Download PDFInfo
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- CN104614345A CN104614345A CN201510031015.9A CN201510031015A CN104614345A CN 104614345 A CN104614345 A CN 104614345A CN 201510031015 A CN201510031015 A CN 201510031015A CN 104614345 A CN104614345 A CN 104614345A
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Abstract
The invention belongs to the field of biochemical sensing, and relates to a portable optical fiber SPR (Surface Plasma Resonance) image sensor. By utilizing the principle of surface plasma resonance, and by connecting an ordinary computer, SPR detection can be carried out at all times and places. The technical scheme is as follows: utilizing an LED light source to form monochromatic light; light passes through the optical fiber packaging a flowing cell; by special treatment, the optical fiber at the position of the flowing cell can generate the SPR phenomena; the flowing cell has the function of facilitating detection of a biochemical liquid, and real-time measurement can be realized; the light passing through the optical fiber is accepted by a tail-end camera, the camera is connected with the computer, and light spots are displayed on a computer screen; by programming, the measurement on the light spot intensity can be realized; that is to say, the system can transform information of the biochemical liquid into information of the light spot intensity, and can conveniently and fast carry out SPR biochemical detection at all times and places. The image sensor has the benefits that the image sensor can carry out SPR biochemical detection at all times and places, and is simple, fast and low in cost.
Description
Technical field
The invention belongs to a kind of biochemical sensitive technical field, relate to the principle of surface plasma body resonant vibration (SPR).
Background technology
Surface plasma resonance (Surface Plasmon Resonance, SPR) sensor is a kind of novel photoelectric detecting technology, its crucial probe portion is a sensitizing range based on surface plasmon resonance effect, when incident light is when meeting certain condition, cause the resonance of metal free electron, thus make the reflected light of specific wavelength obviously weaken to realize sensing effect.Compared with traditional sensor, it has the advantages such as equipment is simple, highly sensitive, good stability, fast response time, be widely used at many key areas such as medical science, biology, environment, food inspection, pharmacy and move to maturity gradually, there is good development prospect.
At present, lens type spr sensor has had commercial finished product instrument abroad, and also can realize hyperchannel on-line checkingi, but due to its complex structure, bulky, be not suitable for portable type measuring etc., application is very restricted.The unique advantage such as itself small size, high flexibility and cost of optical fiber is low, becomes a kind of developing direction of portable sensor.Optical fiber SPR sensor, compared with glass prism SPR sensor, has highly sensitive, fast response time, and dynamic range is large, anti-electromagnetic interference (EMI), and superelevation insulate, and passivity, be suitable for real-time detection, volume is little, dirigibility advantages of higher.
But current optical fiber SPR sensor needs to use in the lab, no matter wavelength SPR, polarization SPR, angle SPR or image SPR needs the experiment equipments such as light source, (FBG) demodulator, CCD, cost is higher, and higher to requirement for experiment condition.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of portable fiber-optic SPR imageing sensor, optical fiber element is used to substitute prism element, significantly simplify light path and control system, the LED using family common and IP Camera are as light source and detector, whole system structure is simple, with low cost, may be used for the quick detection carrying out biological sample.This sensor does not need large-scale experimental apparatus, is easy to carry.Damage can not be produced to sample, low to the requirement of working environment.This sensor can not only realize biochemistry detection, and adds reference channel, guarantees the accuracy of experimental result.
The technical solution adopted in the present invention is: a kind of portable fiber-optic SPR imageing sensor, and this portable fiber-optic SPR imageing sensor comprises LED light source, fiber sensing element, flow cell and detector; LED light source, fiber sensing element and detector are in sustained height, and LED light source and detector lay respectively at the two ends of fiber sensing element;
Fiber sensing element adopts multimode plastic-clad optical fiber, is encapsulated in flow cell; Two optical fiber of encapsulation, structure is as follows:
After (1) multimode plastic-clad optical fiber removes middle part 5-10mm plastic envelope, the golden film of plating 45-55nm thickness, installs flow cell in the position of plated film;
(2) another root optical fiber is as reference passage, and two end face utilizes grinding technique to carry out microstructure process and is made into plane, and one end is aimed at LED, and the other end is aimed at the detector being connected to computing machine;
Light sends from LED light source, successively by optical fiber and fiber sensing element, is detected device and catches and synthetic image, changed, realize the detection to testing sample by the light intensity of computer calculate image from the injection of the fiber sensing element other end.
Described LED light source adopts wavelength to be 590-650nm light source.
Described detector adopts IP Camera.
In described fiber sensing element, adopt plastic optical fiber length to be 7 ㎝, the diameter of fibre core and covering is respectively 400 μm and 430 μm of numerical apertures are 0.37.
Effect of the present invention and benefit can carry out SPR biochemistry detection whenever and wherever possible, simple and fast, and with low cost.
Accompanying drawing explanation
Fig. 1 is portable fiber-optic SPR image sensor system schematic diagram.
Fig. 2 is LED light source schematic diagram.
Fig. 3 is flow cell structural drawing.
Fig. 4 is optical fiber structure figure.
Fig. 5 is two-channel compensation schematic diagram.
Fig. 6 is measuring system schematic diagram.
Fig. 7 is the curve synoptic diagram of test liquid variations in refractive index.
Fig. 8 is test Con A and the curve synoptic diagram of RNase B in conjunction with situation.
In figure: 1LED light source; 2 optical fiber; 3 flow cells; 4 detectors; 5USB hub;
6USB line; 7 slide rheostats; 8USB interface; 9 flexible pipes; 10 coverings; 11 evanescent fields;
12 surface plasma waves; 13 gold medal films; 14 testing samples; 15 test channel; 16 reference channels;
17 peristaltic pumps; 18SPR image sensing; 19 computing machines; 20 containers.
Embodiment
The specific embodiment of the present invention is described in detail below in conjunction with technical scheme and accompanying drawing.
1. instrument
(1) centre wavelength is the common red LED light source of 625nm, as Fig. 2.
The simple circuit board of (2) one blocks design, is connected with LED light source, realizes the adjustment that LED light is strong.
(3) liquid communication ponds, as Fig. 3.
(3) computing machines, with a camera.
(4) optical fiber sensing element, material is plastic optical fiber HPOF, Hewlett-Packard 400/430-37/730E).Length is 7 ㎝, and the diameter of fibre core and covering is respectively 400 μm and 430 μm of numerical apertures are 0.37.
2. apparatus and process
Utilize grinding technique to carry out microstructure process to the end face of plastic optical fiber, two end faces are made into plane.Then remove the covering of 5mm in the middle part of an optical fiber, use coating machine to plate the golden film of 50nm, install flow cell in the position of plated film, as shown in Figure 4.Another root optical fiber two ends polish, as reference passage.Two fibre-optic packages are in flow cell, and wherein one end is aimed at LED, and the other end is aimed at camera, as Fig. 5.
3. operate
Proving installation as shown in Figure 6.Use the red LED lamp pearl that wavelength is 625nm, 300,000 pixel home network cameras, the fibre core be encapsulated in flow cell is 400 μm of multimode optical fiber SPR sensing elements, home computer, wriggling flexible pipe and peristaltic pump.Testing sample is con A, and the concentration of sample is respectively 0.05mg/mL, 0.1mg/mL and 0.2mg/mL.In implementation process, from the light of LED by entering into camera after optical fiber, two circular light spots can be detected on computers.When passing into the liquid that refractive index becomes large successively in flow cell, SPR phenomenon can be there is in the region of the gold-plated film of optical fiber, along with the rising of liquid refractivity, can find that the other end light intensity of the optical fiber as Measurement channel strengthens gradually, and not have the fiber end face light intensity of plated film there is no marked change.By program, hot spot is processed, the relation of relative intensity (ratio of test channel and reference channel hot spot light intensity) and liquid refractivity can be obtained, thus complete the measurement of refractive index.Gray level image is converted to by coloured image IP Camera captured.In gray level image, the brightness of each image represents with gray-scale value, and gray-scale value can change to 100% (black) from 0% (white).So the intensity of photo can be weighed by the gray-scale value sum of pixels all on picture.As Fig. 7, this system is for testing the solution of different refractivity, obtain good linear response curve, and due to the existence of reference channel, even if when light source there occurs larger fluctuation, still obtain ideal test result, in ranges of indices of refraction be 1.3335-1.3645 interval on obtain the sensitivity of 2.8555/ refractive index.In addition can carry out chemical modification as the optical fiber of test channel makes it have specific recognition function.As Fig. 8, be the canavaline (Con A) of this system Real-Time Monitoring after test channel carries out chemical modification and the schematic diagram of ribonuclease B (RNase B) in conjunction with situation.
Claims (5)
1. a portable fiber-optic SPR imageing sensor, is characterized in that, this portable fiber-optic SPR imageing sensor comprises LED light source, fiber sensing element, flow cell and detector; LED light source, fiber sensing element and detector are in sustained height, and LED light source and detector lay respectively at the two ends of fiber sensing element;
Fiber sensing element adopts multimode plastic-clad optical fiber, is encapsulated in flow cell; Two optical fiber of encapsulation, structure is as follows:
After (1) multimode plastic-clad optical fiber removes middle part 5-10mm plastic envelope, the golden film of plating 45-55nm thickness, installs flow cell in the position of plated film;
(2) another root optical fiber is as reference passage, and two end face utilizes grinding technique to carry out microstructure process and is made into plane, and one end is aimed at LED, and the other end is aimed at the detector being connected to computing machine;
Light sends from LED light source, successively by optical fiber and fiber sensing element, is detected device and catches and synthetic image, changed, realize the detection to testing sample by the light intensity of computer calculate image from the injection of the fiber sensing element other end.
2. portable fiber-optic SPR imageing sensor according to claim 1, is characterized in that, described LED light source adopts wavelength to be 590-650nm light source.
3. portable fiber-optic SPR imageing sensor according to claim 1 and 2, is characterized in that, described detector adopts IP Camera.
4. portable fiber-optic SPR imageing sensor according to claim 1 and 2, is characterized in that, in described fiber sensing element, adopt plastic optical fiber length to be 7 ㎝, the diameter of fibre core and covering is respectively 400 μm and 430 μm of numerical apertures are 0.37.
5. portable fiber-optic SPR imageing sensor according to claim 3, is characterized in that, in described fiber sensing element, adopt plastic optical fiber length to be 7 ㎝, the diameter of fibre core and covering is respectively 400 μm and 430 μm of numerical apertures are 0.37.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510031015.9A CN104614345A (en) | 2015-01-21 | 2015-01-21 | Portable optical fiber SPR (Surface Plasma Resonance) image sensor |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510031015.9A CN104614345A (en) | 2015-01-21 | 2015-01-21 | Portable optical fiber SPR (Surface Plasma Resonance) image sensor |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105466808A (en) * | 2016-01-13 | 2016-04-06 | 中国计量学院 | Liquid refractive index optical fiber sensor based on image analysis and surface plasma resonance |
| CN106442418A (en) * | 2016-09-06 | 2017-02-22 | 大连理工大学 | Low-cost spectroscopic imaging system based on surface plasma resonance |
| CN106841120A (en) * | 2017-03-31 | 2017-06-13 | 丁利 | Suitable for the flow cell of multichannel terminal reflection type optical fiber spr sensor |
| TWI668430B (en) * | 2017-06-13 | 2019-08-11 | 銘傳大學 | Bended plastic optical fiber surface plasmon resonance sensing platform and system for the same and method for the same |
-
2015
- 2015-01-21 CN CN201510031015.9A patent/CN104614345A/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| WEI PENG ETAL: "Compact surface plasmon resonance imaging sensing system based on general optoelectronic components", 《OPTICS EXPRESS》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105466808A (en) * | 2016-01-13 | 2016-04-06 | 中国计量学院 | Liquid refractive index optical fiber sensor based on image analysis and surface plasma resonance |
| CN105466808B (en) * | 2016-01-13 | 2018-08-03 | 中国计量学院 | A kind of liquid refractivity fibre optical sensor based on image analysis and surface plasma body resonant vibration |
| CN106442418A (en) * | 2016-09-06 | 2017-02-22 | 大连理工大学 | Low-cost spectroscopic imaging system based on surface plasma resonance |
| CN106841120A (en) * | 2017-03-31 | 2017-06-13 | 丁利 | Suitable for the flow cell of multichannel terminal reflection type optical fiber spr sensor |
| CN106841120B (en) * | 2017-03-31 | 2023-08-25 | 丁利 | Flow cell suitable for multichannel terminal reflection type optical fiber SPR sensor |
| TWI668430B (en) * | 2017-06-13 | 2019-08-11 | 銘傳大學 | Bended plastic optical fiber surface plasmon resonance sensing platform and system for the same and method for the same |
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Application publication date: 20150513 |