CN105374265B - A kind of high performance liquid chromatograph experimental teaching model of cobalt and experimental method - Google Patents
A kind of high performance liquid chromatograph experimental teaching model of cobalt and experimental method Download PDFInfo
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- CN105374265B CN105374265B CN201510934294.XA CN201510934294A CN105374265B CN 105374265 B CN105374265 B CN 105374265B CN 201510934294 A CN201510934294 A CN 201510934294A CN 105374265 B CN105374265 B CN 105374265B
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- G—PHYSICS
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- G09B—EDUCATIONAL OR DEMONSTRATION APPLIANCES; APPLIANCES FOR TEACHING, OR COMMUNICATING WITH, THE BLIND, DEAF OR MUTE; MODELS; PLANETARIA; GLOBES; MAPS; DIAGRAMS
- G09B23/00—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes
- G09B23/24—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes for chemistry
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- G—PHYSICS
- G09—EDUCATION; CRYPTOGRAPHY; DISPLAY; ADVERTISING; SEALS
- G09B—EDUCATIONAL OR DEMONSTRATION APPLIANCES; APPLIANCES FOR TEACHING, OR COMMUNICATING WITH, THE BLIND, DEAF OR MUTE; MODELS; PLANETARIA; GLOBES; MAPS; DIAGRAMS
- G09B23/00—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes
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Abstract
The invention discloses a kind of high performance liquid chromatograph experimental teaching model of cobalt and experimental method, including testing cabinet, peripheral accessory system, computer and the emulation chromatograph system in computer；The experiment cabinet is by sample injection unit, detection unit, communication unit, switch and indicator lamp groups into the peripheral accessory system is made of sample holes, injector, water pipe, transparent glass tube, waste liquid bottle and liquid storage bottle；The computer is connected by telecommunication circuit with experiment cabinet.It is of the invention succinct, conveniently, it is economical, directly perceived, can coordinate with analogue system, expensive instrument and equipment is not required, tap water can be used directly in drug, need not prepare sample.The simulation operations of whole simulation are can be realized as, student is helped to further understand instrument operation principle, grasp the operation of instrument, improve students ' practical ability and quality of instruction.
Description
Technical field
The present invention relates to a kind of teaching mode and experimental methods, are specifically a kind of high performance liquid chromatograph experimental teaching model of cobalt
And experimental method, belong to instructional technology field.
Background technology
High performance liquid chromatograph is to be applied to chemical, the common instrument of pharmacy and its relevant speciality, the costliness of instrument and equipment,
University owning amount is less, and improper operation easily causes instrument damage.In undergraduate course, many students cannot move
The chance of hand, student can not understand thoroughly wherein structure and operation principle, it more difficult to grasp specific application method and operation.
The content of the invention
The present invention provides a kind of high performance liquid chromatograph experimental teaching model of cobalt and experimental method, and expensive instrument is not required
Device equipment, drug coordinate with simulation operations software, it becomes possible to realize the simulation operations of whole simulation, classmates is helped to understand instrument
Device operation principle grasps the operation of instrument.
To achieve the above object, a kind of high performance liquid chromatograph experimental teaching model of cobalt of the present invention, including testing cabinet, periphery
Accessory system, computer and the emulation liquid chromatographic system in computer；
The experiment cabinet is by sample injection unit, detection unit, communication unit, switch and indicator lamp groups into the sample introduction list
Sample introduction circuit, detection circuit and telecommunication circuit are respectively equipped in member, detection unit and communication unit；The switch and sample introduction circuit
Electrical connection；The indicator light is connected with detection circuit；
The peripheral accessory system by sample holes, injector, the first water pipe, the second water pipe, the 3rd water pipe, the 4th water pipe,
Transparent glass tube, waste liquid bottle and liquid storage bottle composition；The liquid storage bottle is connected by the first water pipe with sample introduction circuit, the sample introduction electricity
Road is connected by the 3rd water pipe with transparent glass tube, and the transparent glass tube upper opening is connected by the 4th water pipe and sample holes
It connects, the injector carries out liquid feeding into transparent glass tube by sample holes, passes through the second water pipe below the transparent glass tube
Into detection circuit；
The computer is connected by telecommunication circuit with experiment cabinet.
Further, the sample introduction circuit includes peristaltic pump, motor control module and regulated power supply；The detection circuit includes
Infrared tube detection device and indicator light；The telecommunication circuit includes singlechip controller and communication module；
The regulated power supply is electrically connected with switch, and the switch is electrically connected with peristaltic pump, and the peristaltic pump is controlled with motor
Module is electrically connected, and the motor control module is electrically connected with singlechip controller, and the singlechip controller passes through communication module
It is connected with computer, the infrared tube detection device and indicator light are electrically connected with singlechip controller；The peristaltic pump water suction
Pipe is connected by the first water pipe with liquid storage bottle, and the wriggling water pump outlet pipe is connected by the 3rd water pipe with transparent glass tube.
Further, sample introduction circuit further includes relay, and the relay is electrically connected with peristaltic pump.
Further, injector is syringe.
A kind of experimental method of high performance liquid chromatograph experimental teaching model of cobalt, the emulation chromatograph system include real
Drug unit, mobile phase ratio unit, database, image-display units, initializers communication unit are tested, specific method is such as
Under：
1) emulation chromatograph system is opened on computers, in the sample name that experimental drug Unit selection will test
Claim；
2) mobile phase ratio is set in mobile phase ratio unit；
3) switch on experiment cabinet is pressed, communication module is powered for singlechip controller, and regulated power supply controls for motor
Module for power supply, and system communication is initialized, emulation chromatograph system is established with singlechip controller and communicated；The communication mould
Block is usb communication line；
4) after establishing communication, emulation chromatograph system is to the letter of one driving motor module work of singlechip controller
Number, singlechip controller control motor control module operating, so as to which peristaltic pump be driven to start to work, simultaneity factor is by step (1)
In information be sent to singlechip controller, singlechip controller is according to the mobile phase ratio of reading, regulation motor control module
Duty cycle, and then adjust peristaltic pump rotating speed, reach setting value so as to fulfill the ratio of mobile phase；
5) after wriggling pump work, the mobile phase in liquid storage bottle, which enters peristaltic pump by the first water pipe and passes through the 3rd water pipe, to be arrived
Up to transparent glass tube, sample to be tested is injected into transparent glass tube with injector at this time, after sample to be tested is mixed with flowing
Into detection unit, it can determine whether liquid or current by if so, then positioned at the infrared tube detection device of detection unit
Infrared tube detection device can send signal, and after microcontroller control mould receives signal, control indicator light length is bright；Meanwhile monolithic
Machine controller transmits a signal to the emulation chromatograph system in computer by communication module, and system reception has liquid to lead to
It after the signal crossed, can read according to the sample and mobile phase ratio set, the corresponding data of reading database, check corresponding
The results such as laboratory report, dynamic wave peak figure.
The present invention is connected to motor control by regulated power supply connecting valve, relay, relay connection peristaltic pump, peristaltic pump
On molding block, the suction side of peristaltic pump is connected in liquid storage bottle, and motor control module and communication module are connected to SCM system
On, the communication of model and emulating operating system is realized, can peristaltic pump be adjusted according to the difference of emulating operating system parameter setting
Rotating speed, and then adjust sample introduction ratio, preferably reached experiment effect.This experimental model and experimental method be succinct, conveniently, warp
It helps, is directly perceived, coordinating with analogue system, expensive instrument and equipment is not required, tap water can be used directly in drug, need not make
Standby sample, it becomes possible to realize the simulation operations of whole simulation, student is helped to further understand instrument operation principle, grasps instrument
Operation improves students ' practical ability and quality of instruction.
Description of the drawings
Fig. 1 is overall structure diagram of the present invention；
Fig. 2 is the electric functional block diagram of the present invention.
Specific embodiment
The invention will be further described below in conjunction with the accompanying drawings.
As depicted in figs. 1 and 2, a kind of high performance liquid chromatograph experimental teaching model of cobalt of the present invention, including testing cabinet 1, outside
Enclose accessory system, computer and the emulation chromatograph system in computer；
The experiment cabinet 1 is by sample injection unit 1-1, detection unit 1-2, communication unit 1-3, switch 1-4 and indicator light 1-5
It forms, sample introduction circuit, detection circuit are respectively equipped in the sample injection unit 1-1, detection unit 1-2 and communication unit 1-3 and lead to
Believe circuit；The switch 1-4 is electrically connected with sample introduction circuit；The indicator light 1-5 is connected with detection circuit, and indicator light 1-5 can sentence
Whether disconnected detection circuit has sample to flow through；
The peripheral accessory system is by sample holes 2-1, injector 2-2, the first water pipe 2-3, the second water pipe 2-31, the 3rd water
Pipe 2-33, the 4th water pipe 2-34, transparent glass tube 2-4, waste liquid bottle 2-5 and liquid storage bottle 2-6 compositions；The liquid storage bottle 2-6 passes through
First water pipe 2-3 is connected with sample introduction circuit, and the sample introduction circuit is connected by the 3rd water pipe 2-33 with transparent glass tube 2-4, institute
It states transparent glass tube 2-4 upper openings to be connected with sample holes 2-1 by the 4th water pipe 2-34, the injector 2-2 passes through sample introduction
Hole 2-1 carries out liquid feeding into transparent glass tube 2-4, and the transparent glass tube 2-4 lower sections enter detection by the second water pipe 2-31
Circuit；
The computer is connected by telecommunication circuit with experiment cabinet 1.
As improvement of the present invention to above-mentioned technical proposal, the sample introduction circuit include peristaltic pump, motor control module and
Regulated power supply can adjust rotating speed according to the difference for the mobile phase ratio that emulation chromatograph system is set.
The detection circuit includes infrared tube detection device and indicator light 1-5, has been capable of deciding whether current by leading to
It crosses indicator light 1-5 and shows current working condition；
The telecommunication circuit includes singlechip controller and communication module, and telecommunication circuit realizes emulation liquid chromatograph system
The communication of system and experiment cabinet 1；
The regulated power supply is electrically connected with switch 1-4, and the switch 1-4 is electrically connected with peristaltic pump, the peristaltic pump and electricity
Machine control module is electrically connected, and the motor control module is electrically connected with singlechip controller, and the singlechip controller passes through logical
Letter module is connected with computer, and the model STC89C51 of the singlechip controller and refers to the infrared tube detection device
Show that lamp 1-5 is electrically connected with singlechip controller；The peristaltic pump suction side is connected by the first water pipe 2-3 with liquid storage bottle 2-6, institute
Peristaltic pump water outlet is stated to be connected with transparent glass tube 2-4 by the 3rd water pipe 2-33.
Above-mentioned technical proposal is further improved as the present invention, the sample introduction circuit further includes relay, it is described after
Electric appliance is electrically connected with peristaltic pump, and when curtage is excessive, relay can disconnect automatically, is effectively protected each member in circuit
The safety of part extends the service life of each element.
As the present invention to the preferred embodiment of above-mentioned technology, the injector 2-2 is syringe, convenient for injecting sample
Product, certain injector 2-2 can also be other forms.
A kind of experimental method of high performance liquid chromatograph teaching experiment model, the emulation chromatograph system include
Experimental drug unit, mobile phase ratio unit, database, image-display units, initializers communication unit, specific method is such as
Under：
1) emulation chromatograph system is opened on computers, in the title of experimental drug Unit selection sample to be tested；
2) mobile phase ratio is set in mobile phase ratio unit；
3) the switch 1-4 on experiment cabinet 1 is pressed, communication module is powered for singlechip controller, and regulated power supply is motor
Control module powers and initializes system communication, and emulation chromatograph system is established with singlechip controller and communicated, described logical
Letter module is usb communication line；
4) after establishing communication, emulation chromatograph system is to the letter of one driving motor module work of singlechip controller
Number, singlechip controller control motor control module operating, so as to which peristaltic pump be driven to start to work, simultaneity factor is by step (1)
In information be sent to singlechip controller, singlechip controller is according to the mobile phase ratio of reading, regulation motor control module
Duty cycle, and then adjust peristaltic pump rotating speed, reach setting value so as to fulfill the ratio of mobile phase；
5) after wriggling pump work, the mobile phase in liquid storage bottle 2-6 enters peristaltic pump by the first water pipe 2-3 and passes through the 3rd
Water pipe 2-33 reaches transparent glass tube 2-4, injects sample to be tested into transparent glass tube 2-4 with injector 2-2 at this time, treats test sample
Product enter detection unit 1-2 after being mixed with flowing, and the infrared tube detection device positioned at detection unit 1-2 can determine whether
Have liquid or current by, if so, then infrared tube detection device can send signal, after microcontroller control mould receives signal,
Control indicator light 1-5 long bright；Meanwhile singlechip controller transmits a signal to the emulation liquid phase in computer by communication module
Chromatograph system, system reception have liquid by signal after, can read according to the sample ID and mobile phase ratio that set,
The corresponding data of reading database check the results such as corresponding laboratory report, dynamic wave peak figure.
The present invention realizes the tune of sample introduction ratio by the communication of teaching experiment model and emulation chromatograph system
Section simulates sampling step by the cooperation of injector 2-2 and sample holes 2-1, passes through the design simulation color of transparent glass tube 2-4
Column is composed, facilitates the clear observation experiment principle of classmate, by detection circuit, the design of indicator light 1-4, simulates the ring of chromatography
Section, by testing the design of cabinet 1, is not required the component of the costliness such as real chromatographic column, chromatogram pump, chromatograph, is not required to
The simulation operations that sample is achieved that liquid chromatograph are prepared, after entire link terminates, emulation chromatograph system can
Corresponding dynamic chromatogram is provided, achievees the effect that whole analog simulation, classmates is helped to understand the operation principle of instrument, is grasped
The operation of instrument.
Claims (5)
1. a kind of high performance liquid chromatograph experimental teaching model of cobalt, which is characterized in that including experiment cabinet (1), peripheral accessory system,
Computer and the emulation chromatograph system in computer；
The experiment cabinet (1) is by sample injection unit (1-1), detection unit (1-2), communication unit (1-3), switch (1-4) and refers to
Show that lamp (1-5) forms, sample introduction electricity is respectively equipped in the sample injection unit (1-1), detection unit (1-2) and communication unit (1-3)
Road, detection circuit and telecommunication circuit；The switch (1-4) is electrically connected with sample introduction circuit；The indicator light (1-5) and detection circuit
Connection；
The peripheral accessory system is by sample holes (2-1), injector (2-2), the first water pipe (2-3), the second water pipe (2-31),
Three water pipes (2-33), the 4th water pipe (2-34), transparent glass tube (2-4), waste liquid bottle (2-5) and liquid storage bottle (2-6) composition；It is described
Liquid storage bottle (2-6) is connected by the first water pipe (2-3) with sample introduction circuit, and the sample introduction circuit is by the 3rd water pipe (2-33) and thoroughly
Bright glass tube (2-4) connection, transparent glass tube (2-4) upper opening pass through the 4th water pipe (2-34) and sample holes (2-1)
Connection, the injector (2-2) carry out liquid feeding, the transparent glass tube into transparent glass tube (2-4) by sample holes (2-1)
Detection circuit is entered by the second water pipe (2-31) below (2-4)；
The computer is connected by telecommunication circuit with experiment cabinet (1).
A kind of 2. high performance liquid chromatograph experimental teaching model of cobalt according to claim 1, which is characterized in that the sample introduction electricity
Road includes peristaltic pump, motor control module and regulated power supply；The detection circuit includes infrared tube detection device and indicator light
(1-5)；The telecommunication circuit includes singlechip controller and communication module；
The regulated power supply is electrically connected with switch (1-4), and the switch (1-4) is electrically connected with peristaltic pump, the peristaltic pump and electricity
Machine control module is electrically connected, and the motor control module is electrically connected with singlechip controller, and the singlechip controller passes through logical
Letter module is connected with computer, and the infrared tube detection device and indicator light (1-5) are electrically connected with singlechip controller；It is described
Peristaltic pump suction side is connected by the first water pipe (2-3) with liquid storage bottle (2-6), and the peristaltic pump water outlet passes through the 3rd water pipe
(2-33) is connected with transparent glass tube (2-4).
A kind of 3. high performance liquid chromatograph experimental teaching model of cobalt according to claim 2, which is characterized in that the sample introduction electricity
Road further includes relay, and the relay is electrically connected with peristaltic pump.
A kind of 4. high performance liquid chromatograph experimental teaching model of cobalt according to claim 2, which is characterized in that the injector
(2-2) is syringe.
5. a kind of a kind of such as experimental method of high performance liquid chromatograph experimental teaching model of cobalt of claim 2~4 any one of them,
It is characterized in that, the emulation chromatograph system includes experimental drug unit, mobile phase ratio unit, database, image
Display unit, initializers communication unit, specific method are as follows：
1) emulation chromatograph system is opened on computers, in the title of experimental drug Unit selection sample to be tested；
2) mobile phase ratio is set in mobile phase ratio unit；
3) switch (1-4) on experiment cabinet (1) is pressed, communication module is powered for singlechip controller, and regulated power supply is motor
Control module is powered, and initializes system communication, and emulation chromatograph system is established with singlechip controller and communicated；It is described logical
Letter module is PL2303 modules, and USB turns TTL；
4) after establishing communication, signal of the chromatograph system to one driving motor module work of singlechip controller is emulated,
Singlechip controller control motor control module operating, so as to which peristaltic pump be driven to start to work, simultaneity factor will be in step (1)
Information is sent to singlechip controller, and singlechip controller is accounted for according to the mobile phase ratio of reading, regulation motor control module
Empty ratio, and then the rotating speed of peristaltic pump is adjusted, reach setting value so as to fulfill the ratio of mobile phase；
5) after wriggling pump work, mobile phase in liquid storage bottle (2-6) into peristaltic pump and passes through the 3rd by the first water pipe (2-3)
Water pipe (2-33) reaches transparent glass tube (2-4), and with injector (2-2), into transparent glass tube (2-4), test sample is treated in injection at this time
Product, sample to be tested enter detection unit (1-2) after being mixed with flowing, and the infrared tube positioned at detection unit (1-2) detects
Device determines whether liquid or current by the way that if so, then infrared tube detection device can send signal, microcontroller control mould connects
After receiving signal, control indicator light (1-5) length is bright；Meanwhile singlechip controller transmits a signal to calculating by communication module
Emulation chromatograph system in machine, system reception have liquid by signal after, can read according to the sample name that sets
Title and mobile phase ratio, the corresponding data of reading database, check corresponding laboratory report, dynamic wave peak figure result.
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| CN201510934294.XA CN105374265B (en) | 2015-12-15 | 2015-12-15 | A kind of high performance liquid chromatograph experimental teaching model of cobalt and experimental method |
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| CN105374265B true CN105374265B (en) | 2018-05-22 |
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| CN106198820A (en) * | 2016-08-26 | 2016-12-07 | 中国石油天然气股份有限公司广西石化分公司 | Can intuitively show the gas chromatograph of sample introduction |
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