CN204330775U - Brucella immunochromatographydetecting detecting test strip - Google Patents
Brucella immunochromatographydetecting detecting test strip Download PDFInfo
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- CN204330775U CN204330775U CN201520026357.7U CN201520026357U CN204330775U CN 204330775 U CN204330775 U CN 204330775U CN 201520026357 U CN201520026357 U CN 201520026357U CN 204330775 U CN204330775 U CN 204330775U
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Abstract
The utility model discloses a kind of brucella immunochromatographydetecting detecting test strip, comprise base plate, described base plate side is superimposed with the first sample pad, the first pad, the first chromatographic film, the first adsorptive pads successively by chromatography direction, described first pad is coated with rare-earth fluorescent nano particle-staphylococcal protein A conjugate; Described first chromatographic film is provided with the first detection line being coated with brucella protein molecule and the first nature controlling line being coated with warm-blooded animal IgG.This brucella immunochromatographydetecting detecting test strip has good specificity and sensitivity, and the detection animal brucella that can detect fast simple to operation infects, and also have the advantage of versatility and dirigibility, and low price is suitable for more onsite application.
Description
Technical field
The utility model relates to field of biological detection, is specifically related to the test strips that a kind of immunochromatography technique detects animal brucella infection.
Background technology
Brucellosis (being called for short cloth sick) is the infection-allergic disease of the infecting both domestic animals and human caused by brucella.Cloth disease is acute infectious disease, is defined as Category B notifiable disease by " People's Republic of China's law on the prevention and control of infectious diseases ".The domestic animal infecting cloth disease is the major source of infection of mankind's cloth disease.
Strengthening epidemic monitoring is the key point carrying out brucellosis prevention and control.Regularly the antibody horizontal in animal body being detected, eradicating significant for carrying out epidemic monitoring, animal immunisation effect assessment and disease.China, as a developing populous nation, animal husbandry big country, is badly in need of setting up a set of simple and easy to do animal brucellosis antibody horizontal detection method.
Immunochromatography technique is the Fast Detection Technique based on antigen-antibody reaction, is widely used in clinical detection.But existing immunochromatography technique many employings collaurum, electroselenium, colored latex particles etc. are as tracer, the defect that ubiquity sensitivity is low.Fluorescent nano particle is a class composite nano materials, that the granule interior reaching nanoscale size at diameter is wrapped up into multiple or single luminophore, so not only enhance fluorescence intensity, and enhance anti-interference, the biocompatibility of luminescent material, be extremely suitable for biomarker.Such material has become the focus that the field such as biology, chemistry, physics is pursued.Fluorescent nano particle test strips adopts unique fluorescent nano particle to replace traditional colloid gold particle, is prepared into immuno-chromatographic test paper strip.This type of test strips can show testing result under ultraviolet light, can realize quantitative detection in conjunction with corresponding fluorescence detection device, operation inherits completely the easy Fast Style of colloidal gold strip.The kind of immuno-chromatographic test paper strip is a lot, as detected FOB, HCG, HBsAg, HBsAb, BeAb, HbcAb, HbeAg, LH, PSA immuno-chromatographic test paper strip corresponding different with HIV, and its face shaping is more or less the same, when in the occasion using different types of immuno-chromatographic test paper strip in a large number, for example inspection department, test strips disarrayed and more difficultly correspond to corresponding testing result, consequence impact is serious.
Utility model content
In order to solve above-mentioned the deficiencies in the prior art, the utility model provides a kind of good specificity and sensitivity, and the test strips that the detection animal brucella that simple operations detects fast infects.
Technical problem to be solved in the utility model is achieved by the following technical programs:
Brucella immunochromatographydetecting detecting test strip, comprise base plate, described base plate side is superimposed with the first sample pad, the first pad, the first chromatographic film, the first adsorptive pads successively by chromatography direction, described first pad is coated with rare-earth fluorescent nano particle-staphylococcal protein A conjugate; Described first chromatographic film is provided with the first detection line being coated with brucella protein molecule and the first nature controlling line being coated with warm-blooded animal IgG.
Preferably, described base plate is also provided with the first bar code district or the first Quick Response Code district.
Preferably, described first adsorptive pads is provided with a RFID radio-frequency card.
Preferably, the particle diameter of described rare-earth fluorescent nano particle is 10 ~ 10000nm.
Preferably, one end of the superimposed connection first in one end pad of described first chromatographic film, the other end superimposed connection first adsorptive pads of described first chromatographic film; The other end superimposed connection first sample pad of described first pad.
Preferably, described base plate opposite side is superimposed with the second sample pad, the second pad, the second chromatographic film, the second adsorptive pads successively by chromatography direction, described first chromatographic film is provided with the second detection line and the second nature controlling line.
Preferably, described second pad is the pad being coated with rare-earth fluorescent nano particle-staphylococcal protein A conjugate.
Preferably, described second detection line is the second detection line being coated with brucella protein molecule; Described second nature controlling line is the second nature controlling line being coated with warm-blooded animal IgG.
Preferably, described base plate is also provided with the second bar code district or the second Quick Response Code district.
More preferably, described second adsorptive pads is provided with the 2nd RFID radio-frequency card.
The utility model has following beneficial effect:
The brucella immunochromatographydetecting detecting test strip that the utility model provides has good specificity and sensitivity, and the detection animal brucella that can detect (lower than 20min) fast simple to operation infects; By having bar code or Quick Response Code identification end of scan identification scanning, being conducive to differentiating clear fast for variety classes test strips, being convenient to the application in the occasion that testing is heavy, ensureing reliability and the accuracy of testing result; And cost of manufacture is low, be conducive to applying; Also can have two-layer equation immune chromatography test paper, can identical or different test item be carried out, cost-saving, prevent the waste of resource, and have the advantage of versatility, simplicity, susceptibility, specificity, rapidity and dirigibility, and low price is suitable for more onsite application.
Accompanying drawing explanation
Fig. 1 is the structural representation of embodiment 1 in the utility model;
Fig. 2 is the cross-sectional view of embodiment 2 in the utility model;
Fig. 3 is the structural representation of embodiment 2 in the utility model.
Embodiment
Below in conjunction with drawings and Examples, the utility model is described in detail.
Embodiment 1
As shown in Figure 1, the utility model provides a kind of brucella immunochromatographydetecting detecting test strip, comprises base plate 1, and described base plate 1 preferably but be not defined as PVC board.Described base plate 1 side is superimposed with the first sample pad 11, first pad 12, first chromatographic film 13, first adsorptive pads 14 successively by chromatography direction.The lower surface of described first chromatographic film 13 is fixedly fitted in the upper surface of described base plate 1; Upper surface two ends superimposed connection first pad 12 and first adsorptive pads 14 respectively of described first chromatographic film 13; Described first sample pad 11 of the superimposed connection of the other end of described first pad 12.
Described first pad 12 is for being coated with the glass fibre cotton of rare-earth fluorescent nano particle-staphylococcal protein A conjugate; Described first chromatographic film 13 is provided with the first detection line 131 being coated with brucella protein molecule and the first nature controlling line 132 being coated with warm-blooded animal IgG.Described first chromatographic film 13 preferably but be not defined as nitrocellulose membrane.Preferably but do not limit, described brucella protein molecule is BP26 albumen; Described warm-blooded animal IgG is mouse IgG; The particle diameter of described rare-earth fluorescent nano particle is 10 ~ 10000nm; Containing Eu in described rare-earth fluorescent nano particle
3+, Sm
3+, Dy
3+or Tb
3+deng the chelate that rare earth ion and sequestrant are formed, this chelate can launch characterized fluorescence under ultraviolet excitation.
Make further improvements, described base plate 1 is also provided with the first bar code district or the first Quick Response Code district 15.Described first bar code district or the first Quick Response Code district 15 for recording test item and relevant information, as antigen/antibody and label etc.Described first bar code district or the first Quick Response Code district 15 are positioned on the base plate 1 of one end of close described sample pad or adsorptive pads.
Make further improvements, described first adsorptive pads 14 is provided with a RFID radio-frequency card 16.The detection data message that a described RFID radio-frequency card 16 produces reads by RFID card reader, simple and quick and accurately read and detect data.
By test item and the relevant information of bar code or this test strips of Quick Response Code scanning recognition terminal check, during loading, the reason of chromatography effect, liquid to be detected is flowed through pad by sample pad, is arrived chromatographic film, transparence is presented because suction sample becomes wet, then sample down chromatography arrival detection line place, owing to containing target determinand in sample, then this target determined antigen/antibody by with nature controlling line on wrap quilt material react, form fluorescent nano particle label and be also intercepted at nature controlling line and the luminescence that develops the color; Again by the further reading displayed of data that RFID will detect.
Embodiment 2
Please refer to Fig. 2,3, the basis of embodiment 1 is carried out the immuno-chromatographic test paper strip being modified into two-layer equation, difference from Example 1 is, described base plate 1 opposite side is superimposed with the second sample pad 21, second pad 22, second chromatographic film 23, second adsorptive pads 24 successively by chromatography direction, and described first chromatographic film 13 is provided with the second detection line 231 and the second nature controlling line 232.The lower surface of described second chromatographic film 23 is fixedly fitted in the lower surface of described base plate 1; Upper surface two ends superimposed connection second pad 22 and second adsorptive pads 24 respectively of described second chromatographic film 23; Described second sample pad 21 of the superimposed connection of the other end of described second pad 22.
Preferably but do not limit, described second pad 22 and the second chromatographic film 23 are wrapped quilt material can with described first pad 12 and the second chromatographic film 23 on to wrap the material of quilt identical or different, can be used for detecting identical or different project, also can carry out different encrusting substance quality detection to same project and compare testing result, guarantee accuracy of detection further.
Same, described base plate 1 opposite side is also provided with the second bar code district or the second Quick Response Code district 25.And described second adsorptive pads 24 is provided with the 2nd RFID radio-frequency card 26.
Preferably but do not limit, described second pad 22 is for being coated with the glass fibre cotton of rare-earth fluorescent nano particle-staphylococcal protein A conjugate; Described second chromatographic film 23 is provided with the second detection line 231 being coated with brucella PROTEIN B P26 and the second nature controlling line 232 being coated with brucella outer membrane protein BP26 polyclonal antibody.Described second chromatographic film 23 preferably but be not defined as nitrocellulose membrane.
Use two-layer equation immuno-chromatographic test paper strip, both the waste of disposable test paper slip resource had been avoided, accelerate detection efficiency again and save cost, realize the advantage of the versatility of test strips, simplicity, susceptibility, specificity, rapidity and dirigibility, and low price being suitable for more onsite application.
The above embodiment only have expressed embodiment of the present utility model; it describes comparatively concrete and detailed; but therefore can not be interpreted as the restriction to the utility model the scope of the claims; in every case the technical scheme adopting the form of equivalent replacement or equivalent transformation to obtain, all should drop within protection domain of the present utility model.
Claims (10)
1. brucella immunochromatographydetecting detecting test strip, it is characterized in that, comprise base plate, described base plate side is superimposed with the first sample pad, the first pad, the first chromatographic film, the first adsorptive pads successively by chromatography direction, described first pad is coated with rare-earth fluorescent nano particle-staphylococcal protein A conjugate; Described first chromatographic film is provided with the first detection line being coated with brucella protein molecule and the first nature controlling line being coated with warm-blooded animal IgG.
2. brucella immunochromatographydetecting detecting test strip according to claim 1, is characterized in that, described base plate is also provided with the first bar code district or the first Quick Response Code district.
3. brucella immunochromatographydetecting detecting test strip according to claim 1, is characterized in that, described first adsorptive pads is provided with a RFID radio-frequency card.
4. brucella immunochromatographydetecting detecting test strip according to claim 1, is characterized in that, the particle diameter of described rare-earth fluorescent nano particle is 10 ~ 10000nm.
5. brucella immunochromatographydetecting detecting test strip according to claim 1, is characterized in that, one end of the superimposed connection first in one end pad of described first chromatographic film, the other end superimposed connection first adsorptive pads of described first chromatographic film; The other end superimposed connection first sample pad of described first pad.
6. brucella immunochromatographydetecting detecting test strip according to claim 1, it is characterized in that, described base plate opposite side is superimposed with the second sample pad, the second pad, the second chromatographic film, the second adsorptive pads successively by chromatography direction, and described first chromatographic film is provided with the second detection line and the second nature controlling line.
7. brucella immunochromatographydetecting detecting test strip according to claim 6, is characterized in that, described second pad is the pad being coated with rare-earth fluorescent nano particle-staphylococcal protein A conjugate.
8. brucella immunochromatographydetecting detecting test strip according to claim 6, is characterized in that, described second detection line is the second detection line being coated with brucella protein molecule; Described second nature controlling line is the second nature controlling line being coated with warm-blooded animal IgG.
9. brucella immunochromatographydetecting detecting test strip according to claim 6, is characterized in that, described base plate is also provided with the second bar code district or the second Quick Response Code district.
10. brucella immunochromatographydetecting detecting test strip according to claim 6, is characterized in that, described second adsorptive pads is provided with the 2nd RFID radio-frequency card.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520026357.7U CN204330775U (en) | 2015-01-15 | 2015-01-15 | Brucella immunochromatographydetecting detecting test strip |
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|---|---|---|---|
| CN201520026357.7U CN204330775U (en) | 2015-01-15 | 2015-01-15 | Brucella immunochromatographydetecting detecting test strip |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107389934A (en) * | 2016-06-30 | 2017-11-24 | 中国检验检疫科学研究院 | Bovine brucellosis fluorescent mark immunity chromatograph test strip |
| CN109655614A (en) * | 2019-02-26 | 2019-04-19 | 广州维佰生物科技有限公司 | Ox brucellosis virus fluorescence micro-ball immune chromatography test paper and preparation method thereof and detection method |
| CN111707819A (en) * | 2020-06-22 | 2020-09-25 | 上海雄图生物科技有限公司 | AFB1, ZEN and DON three-index quantitative chromatography test strip |
| CN112718031A (en) * | 2020-06-19 | 2021-04-30 | 上海快灵生物科技有限公司 | Biochemical test paper tube |
-
2015
- 2015-01-15 CN CN201520026357.7U patent/CN204330775U/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107389934A (en) * | 2016-06-30 | 2017-11-24 | 中国检验检疫科学研究院 | Bovine brucellosis fluorescent mark immunity chromatograph test strip |
| CN107389934B (en) * | 2016-06-30 | 2019-10-11 | 中国检验检疫科学研究院 | Bovine brucellosis fluorescent mark immunity chromatograph test strip |
| CN109655614A (en) * | 2019-02-26 | 2019-04-19 | 广州维佰生物科技有限公司 | Ox brucellosis virus fluorescence micro-ball immune chromatography test paper and preparation method thereof and detection method |
| CN112718031A (en) * | 2020-06-19 | 2021-04-30 | 上海快灵生物科技有限公司 | Biochemical test paper tube |
| CN111707819A (en) * | 2020-06-22 | 2020-09-25 | 上海雄图生物科技有限公司 | AFB1, ZEN and DON three-index quantitative chromatography test strip |
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Address after: 350000 national inspection Plaza, No. 312, Hudong Road, Gulou District, Fuzhou, Fujian Co-patentee after: Shenzhen Rui Rui biotechnology Limited by Share Ltd Patentee after: Inspection & Quarantine Technology Center of Fujian Entry-Exit Inspection & Quar Address before: 350000 national inspection Plaza, No. 312, Hudong Road, Gulou District, Fuzhou, Fujian Co-patentee before: Bioeasy Technology, Inc. Patentee before: Inspection & Quarantine Technology Center of Fujian Entry-Exit Inspection & Quar |