CN103529211B - Method for detecting rubella virus, quantum dot-labeled immunochromatographic test paper and preparation method thereof - Google Patents
Method for detecting rubella virus, quantum dot-labeled immunochromatographic test paper and preparation method thereof Download PDFInfo
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- CN103529211B CN103529211B CN201310484759.7A CN201310484759A CN103529211B CN 103529211 B CN103529211 B CN 103529211B CN 201310484759 A CN201310484759 A CN 201310484759A CN 103529211 B CN103529211 B CN 103529211B
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- 241000710799 Rubella virus Species 0.000 title claims abstract description 54
- 239000002096 quantum dot Substances 0.000 title claims abstract description 45
- 238000012360 testing method Methods 0.000 title claims abstract description 25
- 238000002360 preparation method Methods 0.000 title claims description 9
- 238000000034 method Methods 0.000 title abstract description 15
- 239000000020 Nitrocellulose Substances 0.000 claims abstract description 21
- 229920001220 nitrocellulos Polymers 0.000 claims abstract description 21
- 238000003908 quality control method Methods 0.000 claims abstract description 15
- 239000012528 membrane Substances 0.000 claims abstract description 10
- 239000003365 glass fiber Substances 0.000 claims description 26
- 238000001514 detection method Methods 0.000 claims description 24
- 238000004321 preservation Methods 0.000 claims description 14
- 241000283973 Oryctolagus cuniculus Species 0.000 claims description 13
- 241001529936 Murinae Species 0.000 claims description 12
- 230000008878 coupling Effects 0.000 claims description 10
- 238000010168 coupling process Methods 0.000 claims description 10
- 238000005859 coupling reaction Methods 0.000 claims description 10
- 238000013016 damping Methods 0.000 claims description 10
- 239000012530 fluid Substances 0.000 claims description 10
- 238000001035 drying Methods 0.000 claims description 7
- 238000013096 assay test Methods 0.000 claims description 6
- 238000006243 chemical reaction Methods 0.000 claims description 6
- 239000003153 chemical reaction reagent Substances 0.000 claims description 6
- 229910004613 CdTe Inorganic materials 0.000 claims description 4
- 239000011258 core-shell material Substances 0.000 claims description 4
- SBIBMFFZSBJNJF-UHFFFAOYSA-N selenium;zinc Chemical group [Se]=[Zn] SBIBMFFZSBJNJF-UHFFFAOYSA-N 0.000 claims description 4
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 3
- NQTADLQHYWFPDB-UHFFFAOYSA-N N-Hydroxysuccinimide Chemical compound ON1C(=O)CCC1=O NQTADLQHYWFPDB-UHFFFAOYSA-N 0.000 claims description 3
- BIVUUOPIAYRCAP-UHFFFAOYSA-N aminoazanium;chloride Chemical compound Cl.NN BIVUUOPIAYRCAP-UHFFFAOYSA-N 0.000 claims description 3
- 229940098773 bovine serum albumin Drugs 0.000 claims description 3
- 229910052799 carbon Inorganic materials 0.000 claims description 3
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims description 3
- 238000010790 dilution Methods 0.000 claims description 3
- 239000012895 dilution Substances 0.000 claims description 3
- 239000003292 glue Substances 0.000 claims description 3
- 238000000746 purification Methods 0.000 claims description 3
- 230000035945 sensitivity Effects 0.000 abstract description 6
- 238000007689 inspection Methods 0.000 abstract description 5
- 230000001900 immune effect Effects 0.000 abstract description 2
- 229920002678 cellulose Polymers 0.000 abstract 3
- 239000001913 cellulose Substances 0.000 abstract 3
- 239000011521 glass Substances 0.000 abstract 3
- FJWGYAHXMCUOOM-QHOUIDNNSA-N [(2s,3r,4s,5r,6r)-2-[(2r,3r,4s,5r,6s)-4,5-dinitrooxy-2-(nitrooxymethyl)-6-[(2r,3r,4s,5r,6s)-4,5,6-trinitrooxy-2-(nitrooxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-3,5-dinitrooxy-6-(nitrooxymethyl)oxan-4-yl] nitrate Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O)O[C@H]1[C@@H]([C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@@H](CO[N+]([O-])=O)O1)O[N+]([O-])=O)CO[N+](=O)[O-])[C@@H]1[C@@H](CO[N+]([O-])=O)O[C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O FJWGYAHXMCUOOM-QHOUIDNNSA-N 0.000 abstract 2
- 230000002745 absorbent Effects 0.000 abstract 1
- 239000002250 absorbent Substances 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 2
- 210000003734 kidney Anatomy 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000005424 photoluminescence Methods 0.000 description 2
- 206010037844 rash Diseases 0.000 description 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
- 241000867607 Chlorocebus sabaeus Species 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 208000010201 Exanthema Diseases 0.000 description 1
- 241000158526 Nasalis Species 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 230000001808 coupling effect Effects 0.000 description 1
- 241001493065 dsRNA viruses Species 0.000 description 1
- 201000005884 exanthem Diseases 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 238000003317 immunochromatography Methods 0.000 description 1
- 210000003292 kidney cell Anatomy 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 210000003800 pharynx Anatomy 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 201000005404 rubella Diseases 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000010200 validation analysis Methods 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
- G01N2333/08—RNA viruses
- G01N2333/18—Togaviridae; Flaviviridae
- G01N2333/19—Rubella virus
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Abstract
The invention relates to a medimmune inspection method, and particularly relates to quantum dot-labeled immunochromatographic test paper, and a method for detecting rubella virus by adopting an immunological method. According to the quantum dot-labeled immunochromatographic test paper, a glass cellulose membrane A, a quantum dot-labeled rubella virus IgG monoclonal antibody glass cellulose membrane B, a cellulose nitrate membrane and absorbent paper are sequentially bonded on a plastic board from bottom to top, wherein a rubella virus polyclonal antibody and a rabbit-anti-mouse secondary antibody are arranged on one end of the cellulose nitrate membrane so as to form an inspection strip T and a quality control strip C; a quantum dot-labeled rubella virus IgG monoclonal antibody is located at the other end of the glass cellulose membrane B and corresponds to the inspection strip T and the quality control strip C, and the quantum dot-labeled rubella virus IgG monoclonal antibody is located at one end of a sample feeding point. The inspection sensitivity of the method is higher than of the currently used method by about 1000 times.
Description
Technical field
The present invention relates to medimmune inspection method, particularly relate to use quantum dot-labeled immunochromatographyassay assay test, detect the method for rubella virus with immunologic method.
Background technology
Rubella virus is RNA virus, belongs to Togavirus and belongs to.Rubella virus Anti-TNF-α body structure quite stable, only has a kind of Anti-TNF-α build, without hypotype.Only infect the mankind, can grow at rabbit kidney, newborn vole kidney and green monkey kidney cell.Profile is coarse spherical, diameter 50 ~ 70nm, is made up of a single-stranded RNA genome and lipidic shell, includes an electron-dense cores, covers two-layer loose coat.Virus is thermo-labile, loses vigor very soon in 37 DEG C and room temperature, cold-resistant, and-20 DEG C can short-term preservation, and-60 DEG C can relatively stable preservation some months.More weak in the outer viability of human body, responsive to sanitizer.Before eruption and rash step back 5 days, can virus be found in the pharynx nasalis secretion of infant.
At present, conventional detection method is colloidal gold method, although this method detects fast and convenient, easily operate, accuracy rate is lower, and sensitivity is also lower.The detection method of therefore seeking a kind of low price, easy and simple to handle, sensitivity and specificity all higher is problem in the urgent need to address.
Summary of the invention
For the weak point of above-mentioned technology, the invention provides all higher Test paper of a kind of low price, easy and simple to handle, sensitivity and specificity and the method with this detection paper rubella virus.
A kind of quantum dot-labeled immunochromatographyassay assay test, is provided with plastic plate, nitrocellulose filter, glass fibre element film A, the glass fibre element film B of quantum dot-labeled rubella virus IgG monoclonal antibody, thieving paper;
Wherein, described plastic plate is pasted with successively glass fibre element film A, the glass fibre element film B of quantum dot-labeled rubella virus IgG monoclonal antibody, nitrocellulose filter, thieving paper;
Wherein, described nitrocellulose filter one end has rubella virus polyclonal antibody and rabbit against murine two to resist, and forms detection zone T and quality control band C with this;
Wherein, described quantum dot-labeled rubella virus IgG monoclonal antibody is positioned at the other end of glass fibre element film B, corresponding with detection zone T and quality control band C, and quantum dot-labeled rubella virus IgG monoclonal antibody is positioned at sample delivery point one end.
Preferably, described quantum dot is CdTe/ZnSe core-shell quanta dots.
Preferably, described plastic plate is viscosity PVC base plate.
Preferably, the concentration of described rubella virus polyclonal antibody is 0.5g/L.
Preferably, the concentration that described rabbit against murine two is anti-is 1.0g/L.
Preferably, described detection zone T and quality control band C spacing are no less than 5mm.
The preparation method of test paper as above, comprises the steps:
(1) coupling of quantum dot and rubella virus IgG monoclonal antibody:
Get PBS damping fluid 100 ~ 200uL and the surperficial quantum dot being connected with carboxyl of 5 ~ 20uL of 0.01M;
Choose coupling reagent, coupling reagent is selected from hydroxy succinimide, 1-(3-dimethyl aminopropyl)-3 ethyl carbon diamine hydrochloride;
Add rubella virus IgG monoclonal antibody 150 ~ 200uL;
Shaking table reaction 1 ~ 4 hour;
Chromatographic column filters, centrifugal purification;
Bovine serum albumin(BSA) with 1% ~ 5% is closed;
4 DEG C of preservations;
(2) preparation of test paper:
Resist by the PBS damping fluid of 0.05 ~ 0.15M dilution rubella virus polyclonal antibody and rabbit against murine two, 0.5g/L rubella virus polyclonal antibody and 1.0g/L rabbit against murine two are resisted and is sprayed on nitrocellulose filter one end, form detection zone T and quality control band C, T band and C are with interval 5mm, room temperature is dried, nitrocellulose filter is put into PBS damping fluid, close stand-by for 37 DEG C, or dry rear 4 DEG C of preservations;
Quantum dot-labeled rubella virus IgG monoclonal antibody is evenly sprayed in glass fibre membrane B one end, to be with and C is with corresponding, drying at room temperature, 4 DEG C of preservations with the T formed;
Plastic plate glues note glass fibre element film A, the glass fibre element film B of quantum dot-labeled rubella virus IgG monoclonal antibody, nitrocellulose filter, thieving paper successively;
Cut into test paper with test paper cutting knife, after drying, seal preservation.
With described detection paper rubella virus, comprise the steps: and examine sample point sample one end close to rubella virus IgG monoclonal antibody on the test paper assembled, after reaction 5min, observations in uv analyzer.
Compared with prior art, the present invention has the following advantages: owing to adding core-shell quanta dots in prepared test paper, particularly have employed CdTe/ZnSe water-soluble nuclear-shell quantum dot, by water miscible quantum dot and specific by covalent coupling, the photoluminescent property of double antibodies sandwich principle incorporating quantum point is then utilized whether to detect in sample containing object.Under the irradiation of uviol lamp, by observing the photoluminescence line of detection zone and quality control band on immuno-chromatographic test paper strip, judge testing result.The fluorescent characteristic of immunoreactive high specific and quantum dot combines by the present invention, utilizes quantum dot multi-wavelength excitation, high strength fluorescent emission, and emission peak is narrow, peak shape is symmetrical, the fluorescent characteristic that stability of photoluminescence is good, establishes fast, special, easy, sensitive immunochromatography detection method.The object quantitatively detected is reached by observing fluorescence signal.The detection sensitivity height about about 1000 times of the current conventional a kind of method for quick-collaurum of detection sensitivity ratio of the method.
Accompanying drawing explanation
Fig. 1 is process chart prepared by test paper.
Embodiment
Below in conjunction with drawings and Examples, the present invention is described in further detail.
Embodiment 1: a kind of quantum dot-labeled immunochromatographyassay assay test, be provided with plastic plate, nitrocellulose filter, glass fibre element film A, the glass fibre element film B of quantum dot-labeled rubella virus IgG monoclonal antibody, thieving paper, described glass fibre element film A is the glass fibre element film not having the market of point sample is bought;
Wherein, described plastic plate is pasted with successively glass fibre element film A, the glass fibre element film B of quantum dot-labeled rubella virus IgG monoclonal antibody, nitrocellulose filter, thieving paper;
Wherein, described nitrocellulose filter one end has rubella virus polyclonal antibody and rabbit against murine two to resist, and forms detection zone T and quality control band C with this;
Wherein, described quantum dot-labeled rubella virus IgG monoclonal antibody is positioned at the other end of glass fibre element film B, corresponding with detection zone T and quality control band C, and quantum dot-labeled rubella virus IgG monoclonal antibody is positioned at sample delivery point one end.
Preferably, described quantum dot is CdTe/ZnSe core-shell quanta dots.
Preferably, described plastic plate is viscosity PVC base plate.
Preferably, the concentration of described rubella virus polyclonal antibody is 0.5g/L.
Preferably, the concentration that described rabbit against murine two is anti-is 1.0g/L.
Preferably, described detection zone T and quality control band C spacing are no less than 5mm.
Embodiment 2: the preparation method of test paper as above, as shown in Figure 1, comprises the steps:
(1) coupling of quantum dot and rubella virus IgG monoclonal antibody:
Get PBS damping fluid 100 ~ 200uL and the surperficial quantum dot being connected with carboxyl of 5 ~ 20uL of 0.01M;
Choose coupling reagent, coupling reagent is selected from hydroxy succinimide, 1-(3-dimethyl aminopropyl)-3 ethyl carbon diamine hydrochloride;
Add rubella virus IgG monoclonal antibody 150 ~ 200uL;
Shaking table reaction 1 ~ 4 hour;
Chromatographic column filters, centrifugal purification;
Bovine serum albumin(BSA) with 1% ~ 5% is closed;
4 DEG C of preservations;
(2) preparation of test paper:
Resist by the PBS damping fluid of 0.05 ~ 0.15M dilution rubella virus polyclonal antibody and rabbit against murine two, 0.5g/L rubella virus polyclonal antibody and 1.0g/L rabbit against murine two are resisted and is sprayed on nitrocellulose filter one end, form detection zone T and quality control band C, T band and C are with interval 5mm, room temperature is dried, nitrocellulose filter is put into PBS damping fluid, close stand-by for 37 DEG C, or dry rear 4 DEG C of preservations;
Quantum dot-labeled rubella virus IgG monoclonal antibody is evenly sprayed in glass fibre membrane B one end, to be with T and C is with corresponding, drying at room temperature, 4 DEG C of preservations;
Plastic plate glues note glass fibre element film A, the glass fibre element film B of quantum dot-labeled rubella virus IgG monoclonal antibody, nitrocellulose filter, thieving paper successively;
Cut into test paper with test paper cutting knife, after drying, seal preservation.
Wherein, in step (1), the detection method of coupling effect is as follows:
Gel electrophoresis: adopt 0.8 Ago-Gel, voltage is 80V, after electrophoresis 15min, in uv analyzer, observes electrophoresis result.
Dot blotting: by 0.3g/L rubella virus-BSA liquid point on nitrocellulose filter, 0.01mol/L phosphate buffer (the PBS containing 5%BSA is soaked in after drying, 10nmol/L, PH=7.4, containing NaCl 8.5g/L) in, remaining protein binding site on closing membrane, hatches 1h in 37 DEG C, take out after closing, PBS washs.Prepare three identical rubella virus blotting membranes, dry and put into quantum dot-labeled rubella virus IgG monoclonal antibody respectively, quantum dot-BSA and quantum dot solution afterwards, in 37 DEG C of shaking tables reaction 30min, PBS washings.Observations in uv analyzer.
Embodiment 3: with described detection paper rubella virus, comprises the steps: and examines sample point sample one end close to rubella virus IgG monoclonal antibody on the test paper assembled, after reaction 5min, and observations in uv analyzer.Blank is set to the blood of PBS damping fluid and normal person.
Result judges: under C band manifests the prerequisite of red fluorescence band, and the fluorescent belt intensity of visual T band, with blank, fluorescence is more weak, and the concentration represented containing checking matter in liquid to be measured is lower.
Embodiment 4: detection paper validation verification, the rubella virus solution of preparation four kinds of concentration, concentration is respectively 0.5,1.0,2.0,5.0ug/L.Utilize Test paper and chemiluminescence paper box to detect sample, each Concentration Testing 6 times, measure the validity that test paper detects result.As shown in Table 1, result show the detection paper prepared with the present invention out present the positive, and the larger fluorescence intensity of solution concentration is larger.
Table one: the ELISA test strip result of various concentration rubella virus
The above, be only preferred embodiment of the present invention, and professional who are familiar with this art is after understanding technological means of the present invention such as, and natural energy, according to actual needs, is changed under the teachings of the present invention.Therefore all equal changes of doing according to the present patent application the scope of the claims and modification, once should still remain within the scope of the patent.
Claims (2)
1. a quantum dot-labeled immunochromatographyassay assay test, is characterized in that, is provided with plastic plate, nitrocellulose filter, glass fibre element film A, the glass fibre element film B of quantum dot-labeled rubella virus IgG monoclonal antibody, thieving paper;
Wherein, described plastic plate is pasted with from top to bottom successively glass fibre element film A, the glass fibre element film B of quantum dot-labeled rubella virus IgG monoclonal antibody, nitrocellulose filter, thieving paper;
Wherein, described nitrocellulose filter one end has rubella virus polyclonal antibody and rabbit against murine two to resist, and forms detection zone T and quality control band C with this;
Wherein, described quantum dot-labeled rubella virus IgG monoclonal antibody is positioned at one end of glass fibre element film B, corresponding with detection zone T and quality control band C, and quantum dot-labeled rubella virus IgG monoclonal antibody is positioned at sample delivery point one end;
Wherein, described quantum dot is CdTe/ZnSe core-shell quanta dots;
Described plastic plate is viscosity PVC base plate;
The concentration of described rubella virus polyclonal antibody is 0.5g/L;
The concentration that described rabbit against murine two resists is 1.0g/L;
Described detection zone T and quality control band C spacing are no less than 5mm.
2. the preparation method of quantum dot-labeled immunochromatographyassay assay test as claimed in claim 1, is characterized in that, comprise the steps:
(1) coupling of quantum dot and rubella virus IgG monoclonal antibody:
Get PBS damping fluid 100 ~ 200 μ L and the surperficial quantum dot being connected with carboxyl of 5 ~ 20 μ L of 0.01M;
Choose coupling reagent;
Add rubella virus IgG monoclonal antibody 150 ~ 200 μ L;
Shaking table reaction 1 ~ 4 hour;
Chromatographic column filters, centrifugal purification;
Bovine serum albumin(BSA) with 1% ~ 5% is closed;
4 DEG C of preservations;
Wherein, the coupling reagent in step (1) is selected from hydroxy succinimide, 1-(3-dimethyl aminopropyl)-3-ethyl carbon diamine hydrochloride;
(2) preparation of test paper:
Resist by the PBS damping fluid of 0.05 ~ 0.15M dilution rubella virus polyclonal antibody and rabbit against murine two, 0.5g/L rubella virus polyclonal antibody and 1.0g/L rabbit against murine two being resisted is sprayed on nitrocellulose filter, form detection zone T and quality control band C, T band and C are with interval 5mm, room temperature is dried, nitrocellulose filter is put into PBS damping fluid, close stand-by for 37 DEG C, or dry rear 4 DEG C of preservations;
Quantum dot-labeled rubella virus IgG monoclonal antibody is evenly sprayed on glass fibre membrane B, drying at room temperature, 4 DEG C of preservations;
Plastic plate glues note glass fibre element film A, the glass fibre element film B of quantum dot-labeled rubella virus IgG monoclonal antibody, nitrocellulose filter, thieving paper successively;
Cut into test paper with test paper cutting knife, after drying, seal preservation.
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| CN105181661A (en) * | 2015-08-11 | 2015-12-23 | 郑州安图生物工程股份有限公司 | Kit for fluorescent quantitative joint detection of Toxoplasma gondii IgG and IgM antibodies |
| CN105067584A (en) * | 2015-08-11 | 2015-11-18 | 郑州安图生物工程股份有限公司 | Immunochromatographic kit for quantitative detection of RV (rubella virus) IgG (immunoglobulin G) antibody through quantum dots |
| CN106855577B (en) * | 2016-12-29 | 2019-02-12 | 百创汇国际生物科技(武汉)股份有限公司 | A kind of kit of quick detection uPA and PAI-1 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1811449A (en) * | 2006-02-23 | 2006-08-02 | 上海交通大学 | Method for detecting quantum dot mark fast immune chromatographic test paper bar |
| CN101158684A (en) * | 2007-11-06 | 2008-04-09 | 山东省医药生物技术研究中心 | Immune chromatography test paper and preparation method capable of meanwhile detecting ToRCH infection |
| CN101551398A (en) * | 2008-11-26 | 2009-10-07 | 中国计量学院 | Drug residue competition-type quantum dot-labeled immunochromatography assay test-strip and observation device thereof |
| CN102109519A (en) * | 2009-12-29 | 2011-06-29 | 北京库尔科技有限公司 | Rubella virus IgG and IgM antibody joint inspection kit and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1811449A (en) * | 2006-02-23 | 2006-08-02 | 上海交通大学 | Method for detecting quantum dot mark fast immune chromatographic test paper bar |
| CN101158684A (en) * | 2007-11-06 | 2008-04-09 | 山东省医药生物技术研究中心 | Immune chromatography test paper and preparation method capable of meanwhile detecting ToRCH infection |
| CN101551398A (en) * | 2008-11-26 | 2009-10-07 | 中国计量学院 | Drug residue competition-type quantum dot-labeled immunochromatography assay test-strip and observation device thereof |
| CN102109519A (en) * | 2009-12-29 | 2011-06-29 | 北京库尔科技有限公司 | Rubella virus IgG and IgM antibody joint inspection kit and preparation method thereof |
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