CN104880414B - TSH immune chromatography reagent kit and preparation method thereof - Google Patents
TSH immune chromatography reagent kit and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to biological field, be specifically related to TSH immune chromatography reagent kit and preparation method thereof.It is made up of test card, colorimetric card and immune chromatography result interpretation monitor test kit.Described test card structure is: paste loading pad in plastic bottom board one end, one end of loading pad closely crimps the colloidal gold pad containing labelling TSH β subunit specific antibody, colloidal gold pad one end closely crimps celluloid NC film, the detection line T and nature controlling line C being separated from each other it is coated with on NC film, T line is TSH alpha subunit antibody, C line is dynamics, and the other end of NC film connects sample suction pad and forms reagent paper, and reagent paper forms test card in loading plastic clip.
Description
Technical field
The invention belongs to biological field, be specifically related to TSH immune chromatography reagent kit and preparation method thereof.
Background technology
TSH is the first-selected index that congenital hypothyroidism checks.Every about 4 kilobit neonates have a new life
The thyroid function of youngster has serious defect, and other have the defect that more neonate has slightly or degree differs.Almost all of
Developed country includes that China all implements neonate screening, in order to find and treat congenital hypothyroidism.Neonate first
The reference value of shape gland underactivity TSH is typically less than 10 μ IU/mL.
Thyrotropin (TSH) is the thyroid growth of promotion and the hormone of function of pituitary.The TSH of the mankind is one
Planting glycoprotein, containing 211 aminoacid, saccharide accounts for the 15% of whole molecule.Whole molecule is by alpha subunit and β subunit 2
Condition peptide chain forms.TSH promotes thyroid function comprehensively: includes strengthening iodine pump activity, strengthens peroxidase activity, promote
Enter the links such as Elityran synthesis and tyrosine iodate.Pituitary TSH, the rush first on the one hand secreted by hypothalamus
Shape glandular hormone releasing hormone (TRH) promotion impact, another aspect again by Τ 3, the Inhibitory Effect of Τ 4 feedback, the two
Antagonism mutually, they form hypothalamus-adenohypophysis-thyroid axis.Under normal circumstances, the TRH amount of hypothalamus secretion, determine gland
The level of pituitary-thyroid axis feedback regulation.TRH secretion is many, then Τ 3 in blood, Τ 4 level set point high, when Τ 3 in blood,
When Τ 4 exceedes this setting level, then feedback suppression adenohypophysis secretion TSH, and reduce the adenohypophysis sensitivity to TRH, from
And making Τ 3 in blood, Τ 4 level keeps relative constancy.TSH reference value: 2～10 μ IU/mL.In pathological conditions, TSH contains
Amount changes.Increase: primary hypothyroidism, the chronic lymphocytic thyroiditis with hypothyroidism, exogenous rush first shape
Hormone secretion tumor (lung, mammary gland), subacute thyroiditis convalescent period.Take the photograph people's lithium metal, potassium iodide, thyrotrophic hormone(TH) swash
Hormone-releasing hormone can make thyrotropin increase；Lower: caused by nanosom hypothyroidism, non-thyrotropin tumor
Hyperthyroidism, and take in aspirin, 17-hydroxy-11-dehydrocorticosterone etc..
Immunochromatography colloidal gold technique is novel diagnostic techniques, and ultimate principle is as follows: utilize colloid gold label one antigen or anti-
Body, is coated corresponding pairing antigen or antibody on the NC film of reagent, when containing corresponding specific antibody or anti-in sample during detection
Time former, in colloid gold label granule and sample, part combines formation complex, then chromatographs on NC film, more coated antigen
Or antibody capture, form macroscopic detection T line, under certain conditions, the concentration in the power of T line and sample is in just
It is correlated with or negative correlation, thus realizes detection.
Patent CN201220399454 discloses the fast quantification immune chromatography reagent kit of a kind of TSH, its have easy and simple to handle,
Reaction quickly, be suitable for Site Detection and the advantage such as economical and practical.But have yet with gold colloidal strong charge, pass through
Electrostatic Absorption antibody or albumen, under the change of pH, it is easy to causes antibody or the disengaging of albumen and adsorbs antibody or albumen
After gold colloidal unstable, thus cause the stability of this test kit, sensitivity to ensure.
Summary of the invention
An object of the present invention is to provide the sxemiquantitative immunochromatographytest test kit of a kind of TSH, its by test kit by reagent paper
Card, colorimetric card and immune chromatography result interpretation monitor are constituted.
In one embodiment of the invention, described test card structure is: paste loading pad, loading pad in plastic bottom board one end
One end closely crimp the colloidal gold pad containing labelling TSH β subunit specific antibody, colloidal gold pad one end closely crimps nitric acid
Cellulose NC film, NC film is coated with the detection line T and nature controlling line C being separated from each other, and T line is TSH alpha subunit antibody,
C line is dynamics, and the other end of NC film connects sample suction pad and forms reagent paper, and reagent paper forms test card in loading plastic clip.
The NC film of described test card is the porous spline structure film of aperture 9-15 micron, and loading pad is glass fibre membrane or non-woven fabrics,
Sample suction pad is made up of absorbent filter.Described colorimetric card is the cardboard being printed on the different depth redness lines of series in white background, is used for
Contrast to testing result judges.Totally 5, the red lines of the different depths, the most corresponding 3,10,15,25,55 μ IU/mL
TSH concentration.
Described immune chromatography result interpretation monitor is a kind of Systems for optical inspection, for the quantitative judgement to testing result, TSH
Detection range 0-5000 μ IU/mL.
In the present invention, the process of being coated of NC membrane antibody is: with 0.1M pH8.0 phosphate buffer (PBS) by TSH α
Subunit antibody is configured to the solution of 1-3mg/ml, and anti-Mus IgG is configured to the solution of 1-2mg/ml, with spray film instrument at NC film
Ruling respectively with the parameter of 1-1.5 μ l/cm in upper and lower, is coated C, T line, by NC film in drying room, temperature after line
Spending 20-25 DEG C, humidity is less than 30%, is dried 2-5 hour.
Described test card, colloidal gold pad preparation process is: prepare diameter with gold chloride-trisodium citrate reduction method (Frens method)
For the colloidal gold solution of 20nm, take 100ml gold colloidal liquid after having prepared and be placed in beaker, use 0.2M K2CO3It is adjusted to pH7.
5, add 2.0mg TSH β subunit antibody by 100ml colloidal gold solution, then be slowly added 0.2M K2CO3Gold colloidal is molten
Liquid is adjusted to pH9.0, adds K2CO3Time be 5 minutes, be then stirred at room temperature 30 minutes, add final concentration of 10mg/ml
Bovine serum albumin (BSA), 1mg/mlPVP closes 20min, 12000r/m and is centrifuged 30 minutes, abandons supernatant, delays with PBS
Rush liquid (pH value 8.0) to redissolve to 50ml, spread 20cm by 1ml solution2Ratio uniform be layered on glass fibre membrane or non-woven fabrics
On, then put drying room, temperature 20-25 DEG C, humidity is less than 30%, is dried 2-5 hour, makes colloidal gold pad.
In the present invention further carries out scheme, described glass fibre membrane needs through treatment fluid immersion treatment 5 minutes before coating,
Described treatment fluid is the PBS containing 1mg/ml sucrose, and pH value is 9.5.
The assembling process of described test card is: in hothouse, temperature 20-25 DEG C, and humidity is less than 40%, takes plastic bottom board,
The middle part that the most coated NC film is placed on plastic bottom board is pasted, and colloidal gold pad cuts into suitable width, at NC film T
Line side overlap joint colloidal gold pad, takes 1/5 stickup of colloidal gold pad, pastes loading pad at colloidal gold pad opposite side overlap joint, take colloid
1/3 stickup of gold pad；Overlap sample suction pad in NC film C line side, take 1/10 stickup of sample suction pad；Finally will patch with cutter
Good plastic plate is cut into the wide test strips of 3-5mm, reinstalls in plastic clip, forms reagent test strip card.
Described test kit, detection method is: 1) balances detectable and sample to room temperature, takes out test card, keep flat；2)10
μ I serum, plasma sample, draw 20ul sample, join in sample aperture when sample is whole blood, more immediately at the buffer of bottom
Hole adds 100 μ L Sample dilution (normal saline or PBS), with immune chromatography result interpretation monitor in 15-20 minute
Or colorimetric card sxemiquantitative result of determination；4), when instrument judges, after setting instrument relevant parameter, test card is put into storehouse and examine
Surveying, instrument would indicate that the quantified results of sample concentration；5) time by colorimetric card result of determination, by the color of test card T line
Contrasting with the shade of normal line on colorimetric card, sxemiquantitative judges the concentration ranges of sample.
The medicine have the advantages that and a kind of TSH fast quantification immunochromatography utilizing immunochromatography colloidal gold technique to prepare inspection is provided
Test agent box, the TSH content in the semiquantitative detection sample of energy, it is suitable for serum, blood plasma and whole blood sample simultaneously, and is suitable for facing
Single part detection on bed.Have easy and simple to handle, reaction quickly, sensitivity is high, stability is strong, be suitable for Site Detection and economy is real
With etc. advantage.
Detailed description of the invention
To further describe the present invention below in detail.It is pointed out that following description is only to claimed
The illustration of technical scheme, the not any restriction to these technical schemes.Protection scope of the present invention is with claims
The content that secretary carries is as the criterion.
Embodiment 1
TSH α, β subunit specific pairs antibody is purchased from abcam company of the U.S.；Other reagent are purchased from sigma company.
The process of being coated of NC membrane antibody is: prepared by TSH alpha subunit antibody with 0.1M pH8.0 phosphate buffer (PBS)
Become 2mg/ml solution, anti-Mus IgG is configured to the solution of 1.5mg/ml, with spray film instrument in NC film upper and lower with
The parameter of 1.5 μ l/cm is rule respectively, is coated C, T line, by NC film at drying room after line, and temperature 20-25 DEG C, wet
Degree, less than 30%, is dried 4 hours.
Colloidal gold pad preparation process is: prepare the glue of a diameter of 20nm with gold chloride-trisodium citrate reduction method (Frens method)
Body gold solution, takes 100ml gold colloidal liquid and is placed in beaker, use 0.2M K after having prepared2CO3It is adjusted to pH7.5, by 100ml
Colloidal gold solution adds 2.0mg TSH β subunit antibody, then is slowly added 0.2M K2CO3Colloidal gold solution is adjusted to pH9.0,
Add K2CO3Time be 5 minutes, be then stirred at room temperature 30 minutes, add final concentration of 10mg/ml bovine serum albumin
(BSA), 1mg/mlPVP closes 20min, 12000r/m and is centrifuged 30 minutes, abandons supernatant, with PBS (pH value
8.0) redissolving to 50ml, glass fibre membrane is immersed in treatment fluid and processes 5 minutes, treatment fluid is the PBS containing 1mg/ml sucrose
Buffer, pH value is 9.5.Then glass fibre membrane is taken out, spread 20cm by 1ml solution2Ratio uniform be layered on glass
On fibrous membrane, then putting drying room, temperature 20-25 DEG C, humidity is less than 30%, is dried 4 hours, makes colloidal gold pad.
Test card is assembled in hothouse, temperature 20-25 DEG C, and humidity is less than 40%, takes and moulds base plate, is put by the most coated NC film
Put and paste at the middle part of plastic bottom board, colloidal gold pad is cut into suitable width, overlap colloidal gold pad in NC film T line side,
Take 1/5 stickup of colloidal gold pad, paste loading pad at colloidal gold pad opposite side overlap joint, take 1/3 stickup of colloidal gold pad；At NC
Film C line side overlap joint sample suction pad, takes 1/10 stickup of sample suction pad；Finally will post plastic plate with cutter and be cut into 3-5mm width
Test strips, reinstall in plastic clip, formed test card.
Respectively with 3, reagent paper is measured by the TSH standard substance of 10,15,25,55 μ IU/mL, the standard substance of variable concentrations
Demonstrate varying strength colour band, the colour band of respective strengths is printed onto on colorimetric card, complete colorimetric card and prepare.
Embodiment 2 sensitivity test
Test card is measured by the TSH standard substance using 0,0.1,0.5,1.0,2.0,4.0,8.0 μ IU/mL, Mei Genong
Spend parallel carry out five times test.Detection method: 1) detectable and sample are balanced to room temperature, take out test card, keep flat；2)
Accurately draw 10 μ I serum, plasma sample, draw 20ul sample when sample is whole blood, join in sample aperture, then exist immediately
The buffering fluid apertures of bottom adds 100 μ L Sample dilution (PBS), in 10-15 minute, respectively by naked eyes and Biodot
TSR3000 reads bar system and immune chromatography result is carried out interpretation.Naked eyes result of determination by variable concentrations TSH standard substance reagent paper with
The colouring discrimination of negative reagent paper (TSH is 0 μ IU/mL) judges, instrument result of determination passes through variable concentrations TSH standard substance
Whether the OD590 value of reagent paper and negative reagent paper (TSH is 0 μ IU/mL) exists significant difference, and to carry out result of determination the most positive.
The least concentration TSH determining positive findings is the sensitivity of test card.
Contrasting it addition, be prepared for contrasting reagent paper prepare by reagent paper and embodiment 1 respectively, comparative example removes gold colloidal diameter difference
Outward, other are all with embodiment 1.
Concrete outcome is as follows:
| Gold colloidal diameter (nm) | Naked eyes judge sensitivity | Instrument judges sensitivity | |
| Embodiment 1 | 20 | 0.5μIU/mL | 0.1μIU/mL |
| Comparative example 1 | 10 | Nothing | 8.0μIU/mL |
| Comparative example 2 | 30 | 4.0μIU/mL | 2.0μIU/mL |
Embodiment 3 stability test
After test card embodiment 1 prepared is placed 1 month and 3 months respectively at 40 DEG C, measure according to the method for embodiment 2
The sensitivity of reagent paper.
The comparative example of the present embodiment is as follows:
Comparative example 3: colloidal gold pad preparation process is different from embodiment 1, and other are the most same as in Example 1, comparative example 3 gold colloidal
Pad preparation method is as follows: prepare the colloidal gold solution of a diameter of 20nm with gold chloride-trisodium citrate reduction method (Frens method),
Take 100ml gold colloidal liquid after having prepared to be placed in beaker, use 0.2M K2CO3It is adjusted to pH7.5, molten by 100ml gold colloidal
Liquid adds 2.0mg TSH β subunit antibody, is stirred at room temperature 30 minutes, adds final concentration of 10mg/ml bovine serum albumin
(BSA), 1mg/mlPVP closes 20min, 12000r/m and is centrifuged 30 minutes, abandons supernatant, with PBS (pH value
8.0) redissolving to 50ml, glass fibre membrane is immersed in treatment fluid and processes 5 minutes, treatment fluid is the PBS containing 1mg/ml sucrose
Buffer, pH value is 9.5.Then glass fibre membrane is taken out, spread 20cm by 1ml solution2Ratio uniform be layered on glass
On fibrous membrane, then putting drying room, temperature 20-25 DEG C, humidity is less than 30%, is dried 4 hours, makes colloidal gold pad.
Comparative example 4: colloidal gold pad preparation process is different from embodiment 1, and other are the most same as in Example 1, comparative example 4 gold colloidal
Pad preparation method is as follows: prepare the colloidal gold solution of a diameter of 20nm with gold chloride-trisodium citrate reduction method (Frens method),
Take 100ml gold colloidal liquid after having prepared to be placed in beaker, use 0.2M K2CO3It is adjusted to pH7.5, molten by 100ml gold colloidal
Liquid adds 2.0mg TSH β subunit antibody, then is slowly added 0.2M K2CO3Colloidal gold solution is adjusted to pH8.5, adds
K2CO3Time be 5 minutes, be then stirred at room temperature 30 minutes, add final concentration of 10mg/ml bovine serum albumin (BSA),
1mg/mlPVP closes 20min, 12000r/m and is centrifuged 30 minutes, abandons supernatant, redissolves with PBS (pH value 8.0)
To 50ml, glass fibre membrane being immersed in treatment fluid and processes 5 minutes, treatment fluid is the PBS containing 1mg/ml sucrose,
PH value is 9.5.Then glass fibre membrane is taken out, spread 20cm by 1ml solution2Ratio uniform be layered on glass fibre membrane,
Putting drying room, temperature 20-25 DEG C again, humidity is less than 30%, is dried 4 hours, makes colloidal gold pad.
Comparative example 5: colloidal gold pad preparation process is different from embodiment 1, and other are the most same as in Example 1, comparative example 5 gold colloidal
Pad preparation method is as follows: prepare the colloidal gold solution of a diameter of 20nm with gold chloride-trisodium citrate reduction method (Frens method),
Take 100ml gold colloidal liquid after having prepared to be placed in beaker, use 0.2M K2CO3It is adjusted to pH7.5, by 100ml colloidal gold solution
Add 2.0mg TSH β subunit antibody, then be slowly added 0.2M K2CO3Colloidal gold solution is adjusted to pH9.5, adds K2CO3
Time be 5 minutes, be then stirred at room temperature 30 minutes, add final concentration of 10mg/ml bovine serum albumin (BSA),
1mg/mlPVP closes 20min, 12000r/m and is centrifuged 30 minutes, abandons supernatant, redissolves with PBS (pH value 8.0)
To 50ml, glass fibre membrane being immersed in treatment fluid and processes 5 minutes, treatment fluid is the PBS containing 1mg/ml sucrose,
PH value is 9.5.Then glass fibre membrane is taken out, spread 20cm by 1ml solution2Ratio uniform be layered on glass fibre membrane,
Putting drying room, temperature 20-25 DEG C again, humidity is less than 30%, is dried 4 hours, makes colloidal gold pad.
Comparative example 6: colloidal gold pad preparation process is different from embodiment 1, and other are the most same as in Example 1, comparative example 6 gold colloidal
Pad preparation method is as follows: prepare the colloidal gold solution of a diameter of 20nm with gold chloride-trisodium citrate reduction method (Frens method),
Take 100ml gold colloidal liquid after having prepared to be placed in beaker, use 0.2M K2CO3It is adjusted to pH7.5, by 100ml colloidal gold solution
Add 2.0mg TSH β subunit antibody, then be slowly added 0.2M K2CO3Colloidal gold solution is adjusted to pH9.5, adds K2CO3
Time be 5 minutes, be then stirred at room temperature 30 minutes, add final concentration of 10mg/ml bovine serum albumin (BSA),
1mg/mlPVP closes 20min, 12000r/m and is centrifuged 30 minutes, abandons supernatant, redissolves with PBS (pH value 8.0)
To 50ml, spread 20cm by 1ml solution2Ratio uniform be layered on glass fibre membrane, then put drying room, temperature 20-25 DEG C,
Humidity is less than 30%, is dried 4 hours, makes colloidal gold pad.
Comparative example 7: for the test card of preparation in CN201220399454 embodiment.
Result is as follows:
Test card places the sensitivity results after 1 month
| Naked eyes judge sensitivity | Instrument judges sensitivity | |
| Embodiment 1 | 0.5μIU/mL | 0.1μIU/mL |
| Comparative example 3 | 32.0μIU/mL | 16.0μIU/mL |
| Comparative example 4 | 8.0μIU/mL | 4.0μIU/mL |
| Comparative example 5 | 16.0μIU/mL | 8.0μIU/mL |
| Comparative example 6 | 32.0μIU/mL | 16.0μIU/mL |
| Comparative example 7 | 32.0μIU/mL | 16.0μIU/mL |
Test card places the sensitivity results after 3 months
| Naked eyes judge sensitivity | Instrument judges sensitivity | |
| Embodiment 1 | 1.0μIU/mL | 0.5μIU/mL |
| Comparative example 3 | >64.0μIU/mL | 32.0μIU/mL |
| Comparative example 4 | 32.0μIU/mL | 8.0μIU/mL |
| Comparative example 5 | >64.0μIU/mL | >64.0μIU/mL |
| Comparative example 6 | >64.0μIU/mL | >64.0μIU/mL |
| Comparative example 7 | >64.0μIU/mL | >64.0μIU/mL |
Embodiment 4 linear test
Embodiment 1 is prepared by the TSH standard substance using 0,1.0,2.0,4.0,8.0,16.0,32.0 and 64.0 μ IU/mL
Test card is measured, and each concentration is parallel carries out five tests.Biodot TSR3000 is used to read each concentration of bar system measurement
OD590, averages and returns.
Result shows, test card prepared by the present invention is linearly good, R between 1-64.0 μ IU/mL2>0.99。
Present invention merely illustrates some claimed specific embodiments, one of them or more technical scheme
In described technical characteristic can be combined with arbitrary one or more technical schemes, these are combined and the technical side that obtains
Case is also in the application protection domain, combined just as these and the technical scheme that obtains is concrete in the disclosure of invention
As record.
Claims (4)
1. a sxemiquantitative immunochromatographytest test kit for thyrotropin, it is made up of test card, colorimetric card and immune chromatography result interpretation monitor；Described test card structure is: paste loading pad in plastic bottom board one end, one end of loading pad closely crimps the colloidal gold pad containing labelling thyrotropin β subunit specific antibody, colloidal gold pad one end closely crimps celluloid NC film, the detection line T and nature controlling line C being separated from each other it is coated with on NC film, T line is TSH alpha subunit antibody, C line is dynamics, and the other end of NC film connects sample suction pad and forms reagent paper, and reagent paper forms test card in loading plastic clip；
The process of being coated of described NC membrane antibody is: TSH alpha subunit antibody is configured to the solution of 1-3mg/ml with 0.1M pH8.0 phosphate buffer, anti-Mus IgG is configured to the solution of 1-2mg/ml, rule respectively with the parameter of 1-1.5 μ l/cm in NC film upper and lower with spray film instrument, it is coated C, T line, by NC film at drying room after line, temperature 20-25 ° C, humidity is less than 30%, is dried 2-5 hour；
Colloidal gold pad preparation process is: prepare the colloidal gold solution of a diameter of 20nm with gold chloride-trisodium citrate reduction method, takes 100ml gold colloidal liquid and be placed in beaker after having prepared, with 0. 2M K2CO3It is adjusted to pH7. 5, adds 2.0mg TSH β subunit antibody by 100ml colloidal gold solution, then be slowly added 0. 2M K2CO3Colloidal gold solution is adjusted to pH9.0, adds K2CO3Time be 5 minutes, be then stirred at room temperature 30 minutes, add final concentration of 10mg/ml bovine serum albumin, 1mg/mlPVP closes 20min, 12000r/m and is centrifuged 30 minutes, abandons supernatant, redissolves to 50ml with PBS that pH value is 8.0, spreads 20cm by 1ml solution2Ratio uniform be layered on glass fibre membrane, then put drying room, temperature 20-25 ° C, humidity is less than 30%, is dried 2-5 hour, makes colloidal gold pad；
Described glass fibre membrane needs before coating through treatment fluid immersion treatment 5 minutes, and described treatment fluid is the PBS containing 1mg/ml sucrose, and pH value is 9.5.
Sxemiquantitative immunochromatographytest test kit the most according to claim 1, it is characterised in that the NC film of described test card is the porous spline structure film of aperture 9-15 micron, and loading pad is glass fibre membrane or non-woven fabrics, and sample suction pad is made up of absorbent filter；Described colorimetric card is the cardboard being printed on the different depth redness lines of series in white background, for judging the contrast of testing result；Totally 5, the red lines of the different depths, the TSH concentration of the most corresponding 3,10,15,25,55 μ IU/mL.
Sxemiquantitative immunochromatographytest test kit the most according to claim 1, it is characterised in that described immune chromatography result interpretation monitor is a kind of Systems for optical inspection, for the quantitative judgement to testing result, the detection range 0-5000 μ IU/mL of TSH.
Sxemiquantitative immunochromatographytest test kit the most according to claim 1, it is characterized in that, in hothouse, temperature 20-25 ° C, humidity is less than 40%, taking plastic bottom board, the middle part that the most coated NC film is placed on plastic bottom board is pasted, and colloidal gold pad cuts into suitable width, colloidal gold pad is overlapped in NC film T line side, take 1/5 stickup of colloidal gold pad, paste loading pad at colloidal gold pad opposite side overlap joint, take 1/3 stickup of colloidal gold pad；Overlap sample suction pad in NC film C line side, take 1/10 stickup of sample suction pad；Finally with cutter, the plastic plate posted is cut into the wide test strips of 3-5mm, reinstalls in plastic clip, form test card.
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106771264A (en) * | 2016-12-15 | 2017-05-31 | 威海纽普生物技术有限公司 | Thyrotropin assay kit and preparation method |
| CN106680498B (en) * | 2017-01-05 | 2018-03-13 | 广州华弘生物科技有限公司 | A kind of herpes simplex virus I-type and II type antigen joint inspection kit |
| CN106771193B (en) * | 2017-01-05 | 2018-04-06 | 广州华弘生物科技有限公司 | A kind of immunochromatographytest test kit of herpes simplex virus type II IgM antibody |
| CN106841605A (en) * | 2017-03-24 | 2017-06-13 | 南通伊仕生物技术股份有限公司 | A kind of thyrotropic hormone Test paper and preparation method thereof |
| CN106932573A (en) * | 2017-04-28 | 2017-07-07 | 天津医科大学总医院 | Detect immunity colloidal gold test paper strip of thyroglobulin and preparation method thereof |
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| JPH04108397A (en) * | 1990-08-27 | 1992-04-09 | Internatl Reagents Corp | Monoclonal antibody and method for measuring tsh with the same |
| CN102426249A (en) * | 2011-08-31 | 2012-04-25 | 内蒙古科慧生物科技有限责任公司 | Kit for quantitatively determining thyroid stimulating hormone (TSH) and detection method for TSH |
| CN202794191U (en) * | 2012-08-07 | 2013-03-13 | 天津中新科炬生物制药有限公司 | Thyroid stimulating hormone (TSH) quick quantitative immunochromatographic detection kit |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8900881B2 (en) * | 2008-12-30 | 2014-12-02 | Jin Po Lee | Quantitative analyte assay device and method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH04108397A (en) * | 1990-08-27 | 1992-04-09 | Internatl Reagents Corp | Monoclonal antibody and method for measuring tsh with the same |
| CN102426249A (en) * | 2011-08-31 | 2012-04-25 | 内蒙古科慧生物科技有限责任公司 | Kit for quantitatively determining thyroid stimulating hormone (TSH) and detection method for TSH |
| CN202794191U (en) * | 2012-08-07 | 2013-03-13 | 天津中新科炬生物制药有限公司 | Thyroid stimulating hormone (TSH) quick quantitative immunochromatographic detection kit |
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