CN101566629A - Clenobuterol hydrochloride, salbutamol and paylean three joint inspection card and method for processing detecting sample - Google Patents

Clenobuterol hydrochloride, salbutamol and paylean three joint inspection card and method for processing detecting sample Download PDF

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Publication number
CN101566629A
CN101566629A CNA2009100270071A CN200910027007A CN101566629A CN 101566629 A CN101566629 A CN 101566629A CN A2009100270071 A CNA2009100270071 A CN A2009100270071A CN 200910027007 A CN200910027007 A CN 200910027007A CN 101566629 A CN101566629 A CN 101566629A
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China
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sample
salbutamol
test
antibody
clenobuterol hydrochloride
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CNA2009100270071A
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Chinese (zh)
Inventor
周坚
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WUXI ANTI BIOTECH ENGINEERING Co Ltd
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WUXI ANTI BIOTECH ENGINEERING Co Ltd
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Priority to CNA2009100270071A priority Critical patent/CN101566629A/en
Publication of CN101566629A publication Critical patent/CN101566629A/en
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Abstract

The invention relates to a clenobuterol hydrochloride, salbutamol and paylean three joint inspection card and a method for processing a detecting sample, and belongs to the technical field of detection of a beta-receptor stimulating agent, wherein a test strip is arranged inside a shell of the three joint inspection card, and the clenobuterol hydrochloride, salbutamol and paylean three joint inspection card is formed by the adhesion of a sample gasket, a colloidal gold membrane, a cellulose nitrate membrane and a water absorbing membrane to a bearing backboard in turn; the colloidal gold membrane is a glass fiber membrane of a colloidal gold marker containing a clenobuterol hydrochloride antibody, a salbutamol antibody and a paylean antibody; three detection strips are arranged on the cellulose nitrate membrane and contain clenobuterol hydrochloride protein conjugate, salbutamol protein conjugate and paylean protein conjugate respectively; and a quality control strip containing an anti-rabbit antibody or an anti-mouse antibody is arranged. The clenobuterol hydrochloride, salbutamol and paylean three joint inspection card has the advantages of simultaneously detecting the clenobuterol hydrochloride, the salbutamol and the paylean in urine or feed, animal tissue, meat and liver. The inspection card is easy to prepare and quick and convenient to use, saves the detection cost, and has accurate result.

Description

The disposal route of clenobuterol hydrochloride, salbutamol and Ractopamine three test card and test sample thereof
Technical field
The present invention relates to a kind of utensil that detects the beta-receptor activator, particularly relate to the disposal route of a kind of clenobuterol hydrochloride, salbutamol and Ractopamine three test card and test sample thereof.Belong to beta-receptor activator detection technique field.
Background technology
The beta-receptor activator is a class synthetic adrenoreceptor agonists, comprise clenobuterol hydrochloride (clenbuterol), salbutamol (Salbutamol) and Ractopamine (ractopamine), be commonly called as " clenbuterol hydrochloride ", utilize it to have the livestock and poultry of promotion lipolysis, increase the muscle growth effect, as the heavy partitioning agent of nutrition, to improve lean meat percentage, reduce fat deposition and promote growth of animal, illegally used as culturing promoter by some livestock-raising persons.In feed stripped, illegally use the beta-receptor activator and cause consumer's food poisoning to happen occasionally.Clenbuterol hydrochloride is as the feed addictive that promotes growth of animal, raising lean meat percentage; once promoted the use of in China's large tracts of land; but long-term use can make such medicine accumulate in animal foodstuff; the people eaten feed contain the livestock products of beta-receptor activator as the adjuvant feed after; toxicity symptom will appear; muscular tremor, palpitaition, stress, headache, myalgia, dizzy, symptom such as feel sick, vomit, have a fever, tremble can appear usually, in addition dead.Therefore, world many countries forbids in feed using beta-receptor activator such as Clenbuterol to increase the lean meat percentage of animal.The Chinese government has prohibited beta-receptor activators such as using clenobuterol hydrochloride in herding is produced.
At present, prior art mainly relies on chemical apparatuses analysis, enzyme immunoassay kit for the detection of clenobuterol hydrochloride, salbutamol and Ractopamine, the test strip that independent detection clenobuterol hydrochloride, salbutamol and Ractopamine are also arranged, but up to the present, do not detect the appliance of these three kinds of materials simultaneously.
Summary of the invention
The object of the present invention is to provide a kind of appliance that can detect clenobuterol hydrochloride, salbutamol and Ractopamine simultaneously, overcome the prior art defective of individual event detection one by one.
Technical scheme of the present invention: a kind of clenobuterol hydrochloride, salbutamol and Ractopamine three test card, it is provided with test-strips in the test card shell of rectangular flat shell shape, the test card case surface has the fenestra of detection and well, and test-strips (2) is upward pasted sample pad (8), collaurum film (7), nitrocellulose filter (6) and absorbing membrane (9) thereon successively by adhesive sticker by supporting backboard (5) and formed; Supporting backboard middle part stacked stickup nitrocellulose filter (6), one endlap of supporting backboard is put and is pasted absorbing membrane (9), the stacked stickup sample pad of the other end (8), absorbing membrane is inner to overlap with nitrocellulose filter respectively separately with sample pad the inner, at the clinch of sample pad and nitrocellulose filter, insert and put between the two and paste several sections collaurum films (7);
The collaurum film is for containing anti-antibody of clenbuteral hydrochloride, the glass fibre membrane of desertification butylamine alcohol antibody and anti-Ractopamine antibody colloidal gold label, there are three to detect band and a quality control band on the nitrocellulose filter, be successively: contain sour Clenbuterol protein conjugate and detect band (10), contain the salbutamol protein conjugate and detect band (11), contain the Ractopamine protein conjugate and detect band (12), quality control band (13), quality control band is anti-rabbit antibody or anti-mouse antibody, test-strips is inserted in the test card shell (1), sample pad is over against well (4), and nitrocellulose filter is over against detecting fenestra (3).
The disposal route of clenobuterol hydrochloride, salbutamol and Ractopamine three test card test sample, test sample is urine sample, feed sample or animal tissue, meat, liver sample, for various test sample different disposal routes is arranged.
Disposal route at urine sample is: urine, needs to filter through filter paper earlier if urine sample is muddy shape directly as test sample, gets supernatant and makes specimen.
Disposal route at the feed sample is: take by weighing 0.5 gram feed sample, add 1mL sample extract (pH 7.050mmol/L Tris-HCL), in test tube, stirred 1 minute, leave standstill 10 minutes after, get limpid supernatant and make specimen.
Disposal route at animal tissue, meat, liver sample is: get that 1g shreds or the animal tissue of homogenate, meat, liver sample, place the test tube that contains 1mL sample extract (pH 7.050mmol/L Tris-HCL), tighten the pipe lid, behind the jolting sample one minute, through 100 ℃ of water-baths 5 minutes, be cooled to room temperature, get limpid center section and make specimen.
Beneficial effect of the present invention: three test card of the present invention can detect clenobuterol hydrochloride, salbutamol and the Ractopamine that contains in urine or feed, animal tissue, meat, the liver simultaneously, these three test card are easy to preparation and save testing cost, easy to use quick, the result is accurate.
Description of drawings
The shell of Fig. 1 clenobuterol hydrochloride, salbutamol and Ractopamine three test card and test-strips arrangement figure in the enclosure.1, shell, 2, test-strips, 3, detect fenestra, 4, well.
The test-strips structural representation of Fig. 2 clenobuterol hydrochloride, salbutamol and Ractopamine three test card.5, supporting backboard, 6, nitrocellulose filter, 7, the collaurum film, 8, sample pad, 9, absorbing membrane.
Show the trace synoptic diagram on the nitrate glass tunica fibrosa of Fig. 3 clenobuterol hydrochloride, salbutamol and Ractopamine three test card.6, nitrocellulose filter, 10, hydrochloric Clenbuterol protein conjugate detects band, 11, contain the salbutamol protein conjugate and detect band, 12, contain the Ractopamine protein conjugate and detect band, 13, quality control band.
Embodiment
The present invention further specifies as follows in conjunction with specific embodiments referring to accompanying drawing:
Embodiment 1: clenobuterol hydrochloride, salbutamol and Ractopamine three test card
It is that test-strips 2 is set in test card shell 1, and the setting of the structure of test card shell 1 and test-strips 2 is referring to accompanying drawing 1, and test card shell 1 is rectangular flat shell shape shell, long 7cm, and wide 2cm, thick 0.5cm, shell wall thickness 0.1cm is made by engineering plastics.Test card shell 1 is connected with the shell block two halves by cap and constitutes, and the fenestra 3 of detection and well 4 are arranged on test card shell 1 cap.Test-strips 2 is placed in the shell block of test card shell 1.
Test-strips 2 is fillet thin slices of sandwich construction, and sheet is about 6.0cm, the wide about 0.4cm of sheet, and thickness is less than 2.5mm.Test-strips 2 is sandwich constructions: bottom is a supporting backboard 5, is the igelite thin slice, thick about 0.5mm, long 6.0cm, wide 0.4cm; The stacked stickup nitrocellulose filter 6 in supporting backboard 5 middle parts, cellulose nitrate thickness 0.5mm, long 2-2.5cm, wide 0.4cm.Three isolated horizontal demonstration trace bands are arranged on the nitrocellulose filter 6, referring to accompanying drawing 3, article three, show in the trace that article one is to detect to be with 10, hydrochloric Clenbuterol protein conjugate, second detects and is with 11, contain the salbutamol protein conjugate, the 3rd is to detect to be with 12, contains the Ractopamine protein conjugate, article four, be quality control band 13, contain anti-rabbit antibody or anti-mouse antibody.Regulate protein conjugate concentration and be approximately 1mg/mL, spray film when anti-rabbit antibody or anti-mouse antibody concentration are approximately 0.1mg/mL.Stacked stickup absorbing membrane 9 on supporting backboard 5 one ends, stacked stickup sample pad 8 on supporting backboard 5 other ends, absorbing membrane 9 the inners overlap with nitrocellulose filter 6 one ends respectively separately with sample pad 8 the inners, at the clinch of sample pad 8 with nitrocellulose filter 6, insert and put between the two and paste several sections collaurum films 7, collaurum film 7 is for containing the glass fibre membrane of anti-antibody of clenbuteral hydrochloride, desertification butylamine alcohol antibody and anti-Ractopamine antibody colloidal gold label.Collaurum film 7 thick 0.5-0.8mm, long 0.5-0.6cm, wide 0.4cm.Sample pad 8 thick 0.5-0.8mm, long 2cm, wide 0.4cm.Absorbing membrane 9 thick 0.5-0.8mm, long 1.6-2cm, wide 0.4cm.The collaurum film is with behind antibody and the colloid gold particle mark, regulates concentration and is approximately 1D (ODU) coating colloid on glass fibre membrane, and the oven dry back is standby.Collaurum film 7, sample pad 8 and absorbing membrane 9 are 0.1-0.2cm with nitrocellulose filter 6 laps of splice.Absorbing membrane 9 materials are thieving paper, and sample pad 8 materials are water adsorption glass tunica fibrosas.
The disposal route of embodiment 2 test sample
Clenobuterol hydrochloride of the present invention, salbutamol and Ractopamine three test card are used for detecting livestock feed or animal urine sample or animal tissue, meat, whether liver contains clenobuterol hydrochloride, salbutamol and Ractopamine, differentiate the security of herding food in view of the above.
For different test sample, different disposal routes is arranged:
Disposal route at urine sample is: urine, needs to filter through filter paper earlier if urine sample is muddy shape directly as test sample, gets supernatant and makes specimen.
Disposal route at the feed sample is: take by weighing 0.5 gram feed sample, add 1mL sample extract (pH 7.050mmol/L Tris-HCL), in test tube, stirred 1 minute, leave standstill 10 minutes after, get limpid supernatant and make specimen.
Disposal route at animal tissue, meat, liver sample is: get 1 gram and shred or animal tissue, meat, the liver sample of homogenate, place the test tube that contains 1mL sample extract (pH 7.050mmol/L Tris-HCL), tighten the pipe lid, behind the jolting sample 1 minute, through 100 ℃ of water-baths 5 minutes, be cooled to room temperature, get limpid center section and make specimen.
Use clenobuterol hydrochloride, salbutamol and Ractopamine three joint inspections to survey and stick into when going detection, earlier sample liquid to be detected is instilled into the sample pad 8 of clenobuterol hydrochloride, salbutamol and Ractopamine three test card from well, because syphonic effect principle, drive sample liquid to be detected and collaurum film 7 contained anti-antibody of clenbuteral hydrochloride, desertification butylamine alcohol antibody and anti-Ractopamine antibody colloidal gold labels together to nitrocellulose filter 6 diffusions, observations in 5-10 minute.
The key reaction of test card is immunologic antigen and antibody response, the antibody of the colloid gold label that on nitrocellulose filter, moves, on calibration tape respectively with hydrochloric Clenbuterol protein conjugate, contain the salbutamol protein conjugate, contain the Ractopamine protein conjugate, and anti-rabbit antibody of quality control band or anti-mouse antibody reaction, form the brownish red band.In sample, there are corresponding clenobuterol hydrochloride to be checked, salbutamol and Ractopamine to exist, then sample promptly reacts with corresponding antibody after adding, and can be with the contained clenobuterol hydrochloride protein conjugate of relevant detection band, contain the salbutamol protein conjugate, contain the reaction of Ractopamine protein conjugate, thereby do not develop the color.The result of key reaction has following 9 kinds of situations:
If 1 show to detect be with 10,11,12 and quality control band 13 manifest brownish red trace band simultaneously, the expression testing result is negative, illustrates that clenobuterol hydrochloride in the detected sample, salbutamol and Ractopamine content surpass permissible value.
If 2 detect and to be with 10,11,12 Show Colors not, have only quality control band 13 to manifest the brownish red trace, testing result is positive, shows that clenobuterol hydrochloride in the detected sample, salbutamol and Ractopamine content surpass permissible value (5ppb) simultaneously.
If 3 detect and to be with 10,11 Show Colors not, detection be with 12 and quality control band 13 manifest the brownish red trace simultaneously, testing result is positive, show that clenobuterol hydrochloride and salbutamol content surpass permissible value (5ppb) in the detected sample, and Ractopamine content surpasses permissible value (5ppb).
If 4 detect and to be with 10,12 Show Colors not, detection be with 11 and quality control band 13 manifest the brownish red trace simultaneously, testing result is positive, show that clenobuterol hydrochloride and Ractopamine content surpass permissible value (5ppb) in the detected sample, and salbutamol content surpasses permissible value (5ppb).
If 5 detect and to be with 11,12 Show Colors not, detection be with 10 and quality control band 13 manifest the brownish red trace simultaneously, testing result is positive, show that salbutamol and Ractopamine content surpass permissible value (5ppb) in the detected sample, and clenobuterol hydrochloride content surpasses permissible value.
If 6 detect and to be with 10 Show Colors not, detection be with 11,12 and quality control band 13 manifest the brownish red trace simultaneously, testing result is positive, show in the detected sample that clenobuterol hydrochloride content surpasses permissible value (5ppb), and salbutamol, Ractopamine content surpass permissible value (5ppb).
If 7 detect and to be with 11 Show Colors not, detection be with 10,12 and quality control band 13 manifest the brownish red trace simultaneously, testing result is positive, show in the detected sample that salbutamol content surpasses permissible value (5ppb), and clenobuterol hydrochloride, Ractopamine content surpass permissible value (5ppb).
If 8 detect and to be with 12 Show Colors not, detection be with 10,11 and quality control band 13 manifest the brownish red trace simultaneously, testing result is positive, show in the detected sample that Ractopamine content surpasses permissible value (5ppb), and clenobuterol hydrochloride, salbutamol content surpass permissible value (5ppb).
If 9 detect be with 10,11,12 and quality control band 13 all do not develop the color, show that then test-strips lost efficacy.

Claims (2)

1, a kind of clenobuterol hydrochloride, salbutamol and Ractopamine three test card, it is provided with test-strips in the test card shell of rectangular flat shell shape, the test card case surface has the fenestra of detection and well, it is characterized in that test-strips (2) is formed by pasting sample pad (8), collaurum film (7), nitrocellulose filter (6) and absorbing membrane (9) thereon successively by adhesive sticker on the supporting backboard (5); Supporting backboard middle part stacked stickup nitrocellulose filter (6), one endlap of supporting backboard is put and is pasted absorbing membrane (9), the stacked stickup sample pad of the other end (8), absorbing membrane is inner to overlap with nitrocellulose filter respectively separately with sample pad the inner, at the clinch of sample pad and nitrocellulose filter, insert and put between the two and paste several sections collaurum films (7); The collaurum film is for containing anti-antibody of clenbuteral hydrochloride, the glass fibre membrane of desertification butylamine alcohol antibody and anti-Ractopamine antibody colloidal gold label, there are three to detect band and a quality control band on the nitrocellulose filter, be successively: hydrochloric Clenbuterol protein conjugate detects band (10), contain the salbutamol protein conjugate and detect band (11), contain the Ractopamine protein conjugate and detect band (12), quality control band (13), quality control band is anti-rabbit antibody or anti-mouse antibody, test-strips is inserted in the test card shell (1), sample pad is over against well (4), and nitrocellulose filter is over against detecting fenestra (3).
2, use the disposal route of the described clenobuterol hydrochloride of claim 1, salbutamol and Ractopamine three test card test sample, it is characterized in that test sample is urine sample, feed sample or animal tissue, meat, liver sample, has different disposal routes for various test sample;
Disposal route at urine sample is: urine, needs to filter through filter paper earlier if urine sample is muddy shape directly as test sample, gets supernatant and makes specimen;
Disposal route at the feed sample is: take by weighing 0.5 gram feed sample, add 1mL sample extract pH 7.0,50mmol/L Tris-HCL, in test tube, stirred 1 minute, leave standstill 10 minutes after, get limpid supernatant and make specimen;
Disposal route at animal tissue, meat, liver sample is: get that 1g shreds or the animal tissue of homogenate, meat, liver sample, place the test tube that contains 1mL sample extract pH 7.0,50mmol/L Tris-HCL, tighten the pipe lid, behind the jolting sample 1 minute, through 100 ℃ of water-baths 5 minutes, be cooled to room temperature, get limpid center section and make specimen.
CNA2009100270071A 2009-05-22 2009-05-22 Clenobuterol hydrochloride, salbutamol and paylean three joint inspection card and method for processing detecting sample Pending CN101566629A (en)

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CN101915842A (en) * 2010-07-26 2010-12-15 河南省科学院生物研究所有限责任公司 Binary detection test strip of beta-exhilarant ractopamine and salbutamol and preparation method thereof
CN101915841A (en) * 2010-07-26 2010-12-15 河南省科学院生物研究所有限责任公司 Ternary detection test bar of beta-stimulants albuterol, clenobuterol hydrochloride and ractopamine and preparation method thereof
CN101936996A (en) * 2010-07-26 2011-01-05 河南省科学院生物研究所有限责任公司 Binary detection test strip of beta-agonist salbutamol and clenbuterol hydrochioride and preparation method thereof
CN101957381A (en) * 2010-10-15 2011-01-26 无锡安迪生物工程有限公司 Double-channel test card for simultaneously testing estradiol and medroxyprogesterone
CN102043060A (en) * 2010-11-18 2011-05-04 无锡安迪生物工程有限公司 Estradiol, estriol and diethylstilbestrol three-joint detection card and processing method of sample detected by same
CN102175869A (en) * 2011-02-17 2011-09-07 冯晓阳 Tetragenous detection card of clenbuterol, ractopamine, salbutamol and tubertaline and preparation method thereof
CN102253211A (en) * 2011-06-13 2011-11-23 清华大学深圳研究生院 Immune chromatography test paper for detecting clenbuterol hydrochloride and preparation method thereof
CN102288753A (en) * 2011-05-10 2011-12-21 重庆市科学技术研究院 Quadruple colloidal gold immunochromatography testing strip for rapid detection of residual tetracycline, chlortetracycline, oxytetracycline and doxycycline and preparation method of same
CN102297970A (en) * 2011-05-27 2011-12-28 重庆市科学技术研究院 Two-joint detection test strip for rapidly detecting residue of cyromazine (CA) and melamine (MA) and preparation method thereof
CN102305860A (en) * 2011-08-10 2012-01-04 无锡安迪生物工程有限公司 Phenolethanolamine substance rapid detection card and detection method thereof
CN102331499A (en) * 2011-01-27 2012-01-25 无锡安迪生物工程有限公司 Bi-path detecting card capable of simultaneously detecting carbamazepine and sodium valproate and detecting method of same
CN102507944A (en) * 2011-11-09 2012-06-20 河北省科学院生物研究所 Reagent card for semiquantitatively detecting salbutamol
CN102636640A (en) * 2011-03-17 2012-08-15 南通戴尔诺斯生物科技有限公司 Clenbuterol type detection test paper and preparation method thereof
CN102967707A (en) * 2012-03-30 2013-03-13 无锡福阳生物科技有限公司 Triple immune colloidal gold rapid detection card and its preparation and use method
CN103105495A (en) * 2012-09-20 2013-05-15 上海科立特农产品检测技术服务有限公司 Preparation and usage methods of colloidal gold rapid test card for simultaneously detecting three fungaltoxins
CN104237511A (en) * 2014-10-16 2014-12-24 青岛中仁生物科技有限公司 Detection reagent for detecting clenbuterol
CN105092567A (en) * 2015-07-15 2015-11-25 宁波高新区绿邦科技发展有限公司 Clenobuterol hydrochloride-ractopamine-salbutamol combined detection reagent card
CN110609065A (en) * 2019-09-11 2019-12-24 李婉婉 Pregnant stick is tested to electron

Cited By (24)

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CN101936996B (en) * 2010-07-26 2013-05-08 河南省科学院生物研究所有限责任公司 Binary detection test strip of beta-agonist salbutamol and clenbuterol hydrochioride and preparation method thereof
CN101915841A (en) * 2010-07-26 2010-12-15 河南省科学院生物研究所有限责任公司 Ternary detection test bar of beta-stimulants albuterol, clenobuterol hydrochloride and ractopamine and preparation method thereof
CN101936996A (en) * 2010-07-26 2011-01-05 河南省科学院生物研究所有限责任公司 Binary detection test strip of beta-agonist salbutamol and clenbuterol hydrochioride and preparation method thereof
CN101915842A (en) * 2010-07-26 2010-12-15 河南省科学院生物研究所有限责任公司 Binary detection test strip of beta-exhilarant ractopamine and salbutamol and preparation method thereof
CN101915841B (en) * 2010-07-26 2013-01-02 河南省科学院生物研究所有限责任公司 Ternary detection test bar of beta-stimulants albuterol, clenobuterol hydrochloride and ractopamine and preparation method thereof
CN101915842B (en) * 2010-07-26 2013-04-03 河南省科学院生物研究所有限责任公司 Binary detection test strip of beta-exhilarant ractopamine and salbutamol and preparation method thereof
CN101957381A (en) * 2010-10-15 2011-01-26 无锡安迪生物工程有限公司 Double-channel test card for simultaneously testing estradiol and medroxyprogesterone
CN102043060A (en) * 2010-11-18 2011-05-04 无锡安迪生物工程有限公司 Estradiol, estriol and diethylstilbestrol three-joint detection card and processing method of sample detected by same
CN102331499A (en) * 2011-01-27 2012-01-25 无锡安迪生物工程有限公司 Bi-path detecting card capable of simultaneously detecting carbamazepine and sodium valproate and detecting method of same
CN102175869A (en) * 2011-02-17 2011-09-07 冯晓阳 Tetragenous detection card of clenbuterol, ractopamine, salbutamol and tubertaline and preparation method thereof
CN102636640A (en) * 2011-03-17 2012-08-15 南通戴尔诺斯生物科技有限公司 Clenbuterol type detection test paper and preparation method thereof
CN102288753A (en) * 2011-05-10 2011-12-21 重庆市科学技术研究院 Quadruple colloidal gold immunochromatography testing strip for rapid detection of residual tetracycline, chlortetracycline, oxytetracycline and doxycycline and preparation method of same
CN102297970A (en) * 2011-05-27 2011-12-28 重庆市科学技术研究院 Two-joint detection test strip for rapidly detecting residue of cyromazine (CA) and melamine (MA) and preparation method thereof
CN102253211A (en) * 2011-06-13 2011-11-23 清华大学深圳研究生院 Immune chromatography test paper for detecting clenbuterol hydrochloride and preparation method thereof
CN102253211B (en) * 2011-06-13 2013-10-30 清华大学深圳研究生院 Immune chromatography test paper for detecting clenbuterol hydrochloride and preparation method thereof
CN102305860A (en) * 2011-08-10 2012-01-04 无锡安迪生物工程有限公司 Phenolethanolamine substance rapid detection card and detection method thereof
CN102507944A (en) * 2011-11-09 2012-06-20 河北省科学院生物研究所 Reagent card for semiquantitatively detecting salbutamol
CN102967707A (en) * 2012-03-30 2013-03-13 无锡福阳生物科技有限公司 Triple immune colloidal gold rapid detection card and its preparation and use method
CN103105495A (en) * 2012-09-20 2013-05-15 上海科立特农产品检测技术服务有限公司 Preparation and usage methods of colloidal gold rapid test card for simultaneously detecting three fungaltoxins
CN104237511A (en) * 2014-10-16 2014-12-24 青岛中仁生物科技有限公司 Detection reagent for detecting clenbuterol
CN105092567A (en) * 2015-07-15 2015-11-25 宁波高新区绿邦科技发展有限公司 Clenobuterol hydrochloride-ractopamine-salbutamol combined detection reagent card
CN105092567B (en) * 2015-07-15 2019-01-29 宁波高新区绿邦科技发展有限公司 Three link detection reagent card of clenobuterol hydrochloride-Ractopamine-salbutamol
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Application publication date: 20091028