CN201804011U - Dual-channel detection card for simultaneously detecting estradiol and medroxyprogesterone - Google Patents
Dual-channel detection card for simultaneously detecting estradiol and medroxyprogesterone Download PDFInfo
- Publication number
- CN201804011U CN201804011U CN2010205628796U CN201020562879U CN201804011U CN 201804011 U CN201804011 U CN 201804011U CN 2010205628796 U CN2010205628796 U CN 2010205628796U CN 201020562879 U CN201020562879 U CN 201020562879U CN 201804011 U CN201804011 U CN 201804011U
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- estradiol
- sample pad
- detecting
- test
- medroxyprogesterone
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Abstract
A dual-channel detection card for simultaneously detecting estradiol and medroxyprogesterone belongs to the technical field of appliances for detecting female hormones. A detecting window and a sampling hole are arranged on the surface of an outer casing of the detection card; a test strip is arranged in the casing; a nitrocellulose membrane is pasted in the middle of a supporting back plate; a water absorption membrane is pasted to one end of the supporting back plate; a sample pad is pasted to the other end of the supporting back plate; the water absorption membrane and the sample pad are respectively lapped with the nitrocellulose membrane; two colloidal gold membranes are pasted between lapped portions of the sample pad and the nitrocellulose membrane in a clamped manner; the two colloidal gold membranes are respectively glass fiber membranes containing monoclonal antibody colloidal gold markers resistant to estradiol and medroxyprogesterone; and three spaced display print tapes arranged transversely on the nitrocellulose membrane are respectively a detecting tape containing estradiol protein coupling substances, a detecting tape containing medroxyprogesterone protein coupling substances and a quality control tape containing rabbit antibodies or rat antibodies. The detection card is capable of simultaneously detecting two female hormones in one sample, saves detecting cost, and is convenient in use, rapid in detection, high in sensitiveness and accurate in results.
Description
Technical field
The utility model relates to a kind of two-channel detection card that detects estradiol and medroxyprogesterone acetate simultaneously, belongs to the utensil technical field that detects female hormone.
Background technology
Estradiol is widely used sex hormone, because of it can promote growth of animal and improve livestock and poultry lean meat ratio, and application is arranged in animal husbandry.Estradiol can be residual in animal body; be detrimental to health by food chain; Female Children is grown in advance; the male children breast tissue is grown womanlike; unusualization of male reproductive system causes pathology; and cause women with breast cancer and endometriosis incidence to rise, and there is obvious carcinogenicity in estradiol, and developed countries such as America and Europe ban use of estradiol as feed addictive in succession.
Medroxyprogesterone acetate is a kind steroid hormone medicine, has been used for the replacement therapy of menopausal women syndrome disease.Because medroxyprogesterone acetate can obviously promote growth of animal, gone through as feed addictive in states such as the U.S., Australia, New Zealand, but strict withdrawal time has been arranged.Medroxyprogesterone acetate in the food has serious side effects to the people, and the sperm that can suppress the people generates, and causes sterile.Therefore the medroxyprogesterone acetate in the animal food is residual to the mankind, and particularly children's health constitutes potential serious harm.China and European Union forbid making feed addictive with it, but still have the people illegally to use.
At present, prior art mainly contains enzyme immunoassay kit, robotization immunity analysis instrument etc. for the detection of feed addictives such as sex hormone, these method operating process are loaded down with trivial details, the time is long, and operating personnel must have certain specialized technical knowledge and could operate, so range of application is limited.The collaurum rapid detection card that single detection estradiol is also arranged.But up to the present, do not detect the appliance of these two kinds of materials simultaneously.
Summary of the invention
The purpose of this utility model is to provide a kind of utensil that can detect estradiol and medroxyprogesterone acetate simultaneously, the defective that overcomes the prior art complex operation, the specialty requirement is high and can only carries out individual inspiration.
The technical solution of the utility model: the two-channel detection card of a kind of estradiol and medroxyprogesterone acetate, it is in the test card shell (1) of rectangular flat shell shape test-strips (2) to be set, the test card case surface has detection fenestra (3) and well (4), test-strips goes up five kinds of elements of stickup by supporting backboard (5) and forms: supporting backboard middle part stacked stickup nitrocellulose filter (6), stacked stickup absorbing membrane (10) on supporting backboard one end, stacked stickup sample pad (9) on the other end, absorbing membrane is inner to overlap with nitrocellulose filter one end respectively separately with sample pad the inner, clinch at sample pad and nitrocellulose filter, insert and put between the two and paste two sections collaurum films, collaurum film (7) is the glass fibre membrane that contains anti-estradiol antibody colloidal gold label, collaurum film (8) is the glass fibre membrane that contains anti-medroxyprogesterone acetate antibody colloidal gold label, there are 2 to detect band and 1 quality control band on the nitrocellulose filter, article one, detect band (11) and contain the estradiol protein conjugate, another detects band (12) and contains the medroxyprogesterone acetate protein conjugate, and quality control band (13) is anti-rabbit antibody or anti-mouse antibody; Test-strips is inserted in the test card shell, and sample pad is over against well, and nitrocellulose filter is over against detecting fenestra.
The beneficial effects of the utility model: its advantage is the estradiol and the medroxyprogesterone acetate that can detect simultaneously in feed, animal tissue's (muscle and liver etc.), the urine, be easy to preparation and save testing cost, easy to use, detect fast, highly sensitive, the result is accurate.
Description of drawings
The two-channel detection card of Fig. 1 estradiol and medroxyprogesterone acetate and test-strips arrangement figure in the enclosure.1, shell, 2, test-strips, 3, detect fenestra, well 4.
The detection strip structural representation of the two-channel detection card of Fig. 2 estradiol and medroxyprogesterone acetate.5, supporting backboard, 6, nitrocellulose filter, 7, collaurum film (glass fibre membrane that contains anti-estradiol antibody colloidal gold label), 8, collaurum film (glass fibre membrane that contains anti-medroxyprogesterone acetate antibody colloidal gold label), sample pad 9, absorbing membrane 10.
Fig. 3 detects and shows the trace synoptic diagram on the nitrate glass tunica fibrosa of strip.6, nitrocellulose filter, 11, detect band (containing the estradiol protein conjugate), 12, detect band (containing the medroxyprogesterone acetate protein conjugate), 13, quality control band.
Embodiment
The utility model further specifies as follows in conjunction with specific embodiments referring to accompanying drawing:
The two-channel detection card of a kind of estradiol and medroxyprogesterone acetate, it is that test-strips 2 is set in test card shell 1, the setting of the structure of test card shell 1 and test-strips 2 is referring to attached Fig. 1 and 2.Test card shell 1 is rectangular flat shell shape housing, long 7cm, and wide 2cm, thick 0.5cm, shell wall thickness 0.1cm is made by engineering plastics.Test card shell 1 is connected with the shell block two halves by cap and constitutes, and the fenestra 3 of detection and well 4 are arranged on test card shell 1 cap.Test-strips 2 is placed in the shell block of test card shell 1.
Test-strips 2 is fillet thin slices of sandwich construction, and sheet is about 6.0cm, the wide about 0.4cm of sheet, and thickness is less than 2.5mm.Test-strips 2 is sandwich constructions: bottom is a supporting backboard 5, is the igelite thin slice, thick about 0.5mm, long 6.0cm, wide 0.4cm; The stacked stickup nitrocellulose filter 6 in supporting backboard 5 middle parts, cellulose nitrate thickness 0.5mm, long 2-2.5cm, wide 0.4cm.Three isolated horizontal demonstration trace bands are arranged on the nitrocellulose filter 6, show in the trace band referring to 3, three in accompanying drawing, one is to detect to be with 11, contains the estradiol protein conjugate; Another is to detect to be with 12, contains the medroxyprogesterone acetate protein conjugate, and the 3rd is matter pore area 13, contains anti-mouse antibody.Stacked stickup absorbing membrane 10 on supporting backboard 5 one ends, stacked stickup sample pad 9 on supporting backboard 5 the other end, absorbing membrane 10 the inners overlap with nitrocellulose filter 6 one ends respectively separately with sample pad 9 the inners, at the clinch of sample pad 9 with nitrocellulose filter 6, insert and put between the two and paste two sections collaurum films, collaurum film 7 is the glass fibre membranes that contain anti-estradiol antibody colloidal gold label, and collaurum film 8 is the glass fibre membranes that contain anti-medroxyprogesterone acetate antibody colloidal gold label.Collaurum film 7 and collaurum film 8 thick 0.5-0.8mm, long 0.5-0.6cm, wide 0.4cm.Sample pad 9 thick 0.5-0.8mm, long 2cm, wide 0.4cm.Absorbing membrane 10 thick 0.5-0.8mm, long 1.6-2cm, wide 0.4cm.Collaurum film 7, collaurum film 8, sample pad 9 and absorbing membrane 10 are 0.1-0.2cm with the nitrocellulose filter lap of splice.Absorbing membrane 10 materials are thieving paper, and sample pad 9 materials are water adsorption glass tunica fibrosas.
Test-strips 2 is inserted in the shell 1, and sample pad 9 is over against well 4, and nitrocellulose filter 6 is over against detecting fenestra 3.
The key reaction of test card is immunologic antigen and antibody response, the antibody of the colloid gold label that on nitrocellulose filter, moves, on calibration tape respectively with contain estradiol protein conjugate, medroxyprogesterone acetate protein conjugate, and anti-rabbit antibody of quality control band or anti-mouse antibody reaction, form the brownish red band.If have corresponding estradiol to be checked and medroxyprogesterone acetate all to be higher than permissible value in the sample, after adding, sample promptly reacts with corresponding antibody, and not can with the contained estradiol protein conjugate of relevant detection band, the reaction of medroxyprogesterone acetate protein conjugate, thereby do not develop the color.The result of key reaction has following 5 kinds of situations:
If 1 show to detect be with 11,12 and quality control band 13 manifest brownish red trace band simultaneously, the expression testing result is negative, illustrates that estradiol and medroxyprogesterone acetate all are lower than permissible value in the detected sample.
Otherwise 2, detect and to be with 11,12 Show Colors not, have only quality control band 13 to manifest the brownish red trace, testing result is positive, shows that estradiol and medroxyprogesterone acetate all are higher than permissible value in the detected sample.
If 3 detect and to be with 11 Show Colors not, detect be with 12 and quality control band 13 manifest the brownish red trace simultaneously, testing result is positive, show that estradiol is higher than permissible value in the detected sample, and medroxyprogesterone acetate is lower than permissible value.
If 4 detect and to be with 12 Show Colors not, detect be with 11 and quality control band 13 manifest the brownish red trace simultaneously, testing result is positive, show that medroxyprogesterone acetate is higher than permissible value in the detected sample, and estradiol is lower than permissible value.
If 5 detect be with 11,12 and matter pore area 13 all do not develop the color, show that then detecting strip lost efficacy.
Claims (1)
1. the two-channel detection card of estradiol and medroxyprogesterone acetate, it is characterized in that in the test card shell (1) of rectangular flat shell shape, test-strips (2) being set, the test card case surface has detection fenestra (3) and well (4), test-strips goes up five kinds of elements of stickup by supporting backboard (5) and forms: supporting backboard middle part stacked stickup nitrocellulose filter (6), stacked stickup absorbing membrane (10) on supporting backboard one end, stacked stickup sample pad (9) on the other end, absorbing membrane is inner to overlap with nitrocellulose filter one end respectively separately with sample pad the inner, clinch at sample pad and nitrocellulose filter, insert and put between the two and paste two sections collaurum films, collaurum film (7) is the glass fibre membrane that contains anti-estradiol antibody colloidal gold label, collaurum film (8) is the glass fibre membrane that contains anti-medroxyprogesterone acetate antibody colloidal gold label, there are 2 to detect band and 1 quality control band on the nitrocellulose filter, article one, detect band (11) and contain the estradiol protein conjugate, another detects band (12) and contains the medroxyprogesterone acetate protein conjugate, and quality control band (13) is anti-rabbit antibody or anti-mouse antibody; Test-strips is inserted in the test card shell, and sample pad (9) is over against well (4), and nitrocellulose filter (6) is over against detecting fenestra (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010205628796U CN201804011U (en) | 2010-10-15 | 2010-10-15 | Dual-channel detection card for simultaneously detecting estradiol and medroxyprogesterone |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010205628796U CN201804011U (en) | 2010-10-15 | 2010-10-15 | Dual-channel detection card for simultaneously detecting estradiol and medroxyprogesterone |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201804011U true CN201804011U (en) | 2011-04-20 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010205628796U Expired - Fee Related CN201804011U (en) | 2010-10-15 | 2010-10-15 | Dual-channel detection card for simultaneously detecting estradiol and medroxyprogesterone |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201804011U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102297966A (en) * | 2011-05-27 | 2011-12-28 | 天津农学院 | Progesterone semi-quantitative determining colloidal gold test paper and preparation method thereof |
| CN104130980A (en) * | 2014-08-04 | 2014-11-05 | 江南大学 | Anti medroxyprogesterone specific monoclonal antibody hybridoma cell strain and application thereof |
-
2010
- 2010-10-15 CN CN2010205628796U patent/CN201804011U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102297966A (en) * | 2011-05-27 | 2011-12-28 | 天津农学院 | Progesterone semi-quantitative determining colloidal gold test paper and preparation method thereof |
| CN104130980A (en) * | 2014-08-04 | 2014-11-05 | 江南大学 | Anti medroxyprogesterone specific monoclonal antibody hybridoma cell strain and application thereof |
| CN104130980B (en) * | 2014-08-04 | 2016-06-01 | 江南大学 | The one anti-medroxyprogesterone monoclonal antibody specific hybridoma cell strain of strain and application thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110420 Termination date: 20171015 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |