CN203551569U - Joint inspection kit for luteinizing hormone and urinary system infection - Google Patents
Joint inspection kit for luteinizing hormone and urinary system infection Download PDFInfo
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- CN203551569U CN203551569U CN201320690172.7U CN201320690172U CN203551569U CN 203551569 U CN203551569 U CN 203551569U CN 201320690172 U CN201320690172 U CN 201320690172U CN 203551569 U CN203551569 U CN 203551569U
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- 208000015181 infectious disease Diseases 0.000 title claims abstract description 46
- 102000009151 Luteinizing Hormone Human genes 0.000 title claims abstract description 39
- 108010073521 Luteinizing Hormone Proteins 0.000 title claims abstract description 39
- 230000002485 urinary effect Effects 0.000 title claims abstract description 24
- 238000007689 inspection Methods 0.000 title claims abstract description 21
- 229940040129 luteinizing hormone Drugs 0.000 title abstract 7
- 238000001514 detection method Methods 0.000 claims abstract description 36
- 238000011109 contamination Methods 0.000 claims description 21
- 230000000274 adsorptive effect Effects 0.000 claims description 12
- 150000001875 compounds Chemical class 0.000 claims description 2
- 238000012360 testing method Methods 0.000 abstract description 79
- 238000009534 blood test Methods 0.000 abstract description 18
- 210000002700 urine Anatomy 0.000 abstract description 18
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 abstract description 16
- 239000012528 membrane Substances 0.000 abstract description 6
- 238000004587 chromatography analysis Methods 0.000 abstract description 5
- 238000005070 sampling Methods 0.000 abstract description 3
- 238000003317 immunochromatography Methods 0.000 abstract 3
- 210000000265 leukocyte Anatomy 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 239000000243 solution Substances 0.000 description 82
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 63
- 239000000047 product Substances 0.000 description 29
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- 239000010931 gold Substances 0.000 description 22
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- RUFPHBVGCFYCNW-UHFFFAOYSA-N 1-naphthylamine Chemical compound C1=CC=C2C(N)=CC=CC2=C1 RUFPHBVGCFYCNW-UHFFFAOYSA-N 0.000 description 20
- HVBSAKJJOYLTQU-UHFFFAOYSA-N 4-aminobenzenesulfonic acid Chemical compound NC1=CC=C(S(O)(=O)=O)C=C1 HVBSAKJJOYLTQU-UHFFFAOYSA-N 0.000 description 20
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 20
- 238000002331 protein detection Methods 0.000 description 17
- 230000002550 fecal effect Effects 0.000 description 16
- 238000002791 soaking Methods 0.000 description 15
- 239000004094 surface-active agent Substances 0.000 description 15
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- 238000001556 precipitation Methods 0.000 description 12
- 238000002360 preparation method Methods 0.000 description 12
- 239000006228 supernatant Substances 0.000 description 12
- XMNIXWIUMCBBBL-UHFFFAOYSA-N 2-(2-phenylpropan-2-ylperoxy)propan-2-ylbenzene Chemical compound C=1C=CC=CC=1C(C)(C)OOC(C)(C)C1=CC=CC=C1 XMNIXWIUMCBBBL-UHFFFAOYSA-N 0.000 description 10
- WCRQJGJTBROHEA-UHFFFAOYSA-N 2-chloro-2-(1h-indol-3-yl)acetic acid Chemical class C1=CC=C2C(C(Cl)C(=O)O)=CNC2=C1 WCRQJGJTBROHEA-UHFFFAOYSA-N 0.000 description 10
- YRNWIFYIFSBPAU-UHFFFAOYSA-N 4-[4-(dimethylamino)phenyl]-n,n-dimethylaniline Chemical compound C1=CC(N(C)C)=CC=C1C1=CC=C(N(C)C)C=C1 YRNWIFYIFSBPAU-UHFFFAOYSA-N 0.000 description 10
- RMMXTBMQSGEXHJ-UHFFFAOYSA-N Aminophenazone Chemical compound O=C1C(N(C)C)=C(C)N(C)N1C1=CC=CC=C1 RMMXTBMQSGEXHJ-UHFFFAOYSA-N 0.000 description 10
- 229930006000 Sucrose Natural products 0.000 description 10
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 10
- 229960000212 aminophenazone Drugs 0.000 description 10
- UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical compound C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 description 10
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- 210000003651 basophil Anatomy 0.000 description 1
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Abstract
The utility model relates to a joint inspection kit for luteinizing hormone (LH) and urinary system infection and belongs to the technical field of detection. The joint inspection kit comprises an LH immunochromatography test strip and one or more of urine protein test paper, white blood cell test paper, occult blood test paper and nitrite test paper, preferably, a combination pad and a chromatography membrane of the LH immunochromatography test strip are respectively coated with corresponding immunolabelling antibodies and detection antibodies, and the LH immunochromatography test strip and one or more of the urine protein test paper, the white blood cell test paper, the occult blood test paper and the nitrite test paper are arranged in parallel or are arranged on the upper and lower surfaces of a base plate. According to the joint inspection kit, by carrying out self-sampling and once sampling, the LH and the urinary system infection can be simultaneously detected; the joint inspection kit is simple and convenient and is suitable for large-scale popularization and application.
Description
Technical field
The utility model relates to a kind of interstitialcellstimulating hormone (ICSH) and urinary system infection contamination joint inspection kit, and particularly a kind of interstitialcellstimulating hormone (ICSH) (LH) and urinary system infection contamination joint inspection kit, belong to detection technique field.
Background technology
Interstitialcellstimulating hormone (ICSH) (LH) is secreted by adenohypophysis basophil, is present in people's blood and urine, and its effect is the release that stimulates mature egg in ovary.LH concentration in urine significantly rises suddenly about 36 ~ 48 hours conventionally before ovulation, at 14 ~ 28 hours, peaks, and behind peak, approximately 14 ~ 28 hours membrana follicularises break, and discharges mature egg.Women the most easily becomes pregnant in behind LH peak 1 ~ 3 day, thereby, detect the exist level of LH in urine and can be used in the predicting ovulation time.
Urinary system infection contamination is clinical common infectious diseases. women's incidence of disease is apparently higher than the male sex.The long-term urinary system infection contamination that occurs can affect pregnancy, and period of gestation can make the burden of kidney, if kidney dis in illness, consequence can be very serious.
Widespread use colloidal gold method carries out fast detecting to LH clinically, and urinary system infection contamination detects also multiple detection method, comprises urinalysis, bacteriology checking etc., but not yet find the product of LH and urinary system infection contamination joint inspection.As LH and urinary system infection contamination are carried out to joint inspection, oneself's sampling, once can carry out joint inspection, simple and convenient.
Summary of the invention
The purpose of this utility model is to overcome above-mentioned weak point, by primary sample, LH and urinary system infection contamination is detected, and a kind of interstitialcellstimulating hormone (ICSH) (LH) and urinary system infection contamination joint inspection kit are provided, and applies easyly, is suitable for large-scale promotion application.
The technical scheme providing according to the utility model, a kind of interstitialcellstimulating hormone (ICSH) and urinary system infection contamination joint inspection kit, comprise the first base plate, in the one side of the first base plate, is provided with chromatographic film, the front end of chromatographic film arranges opaque diaphragm, and the front end of opaque diaphragm arranges arrow; The front end of arrow arranges pad; In chromatographic film, be also disposed with detection line and nature controlling line, chromatographic film rear end is provided with adsorptive pads.
Also comprise infection index detection zone.
Described infection index detection zone is attached on the second base plate or is compound on any one surface of the first base plate.
In the one side of the first base plate, be provided with chromatographic film, the front end of chromatographic film arranges pad, and the front of pad also arranges sample pad; In chromatographic film, be also disposed with detection line and nature controlling line, chromatographic film rear end is provided with adsorptive pads; On the lower surface of the first base plate, be also provided with infection index detection zone.
Described infection index detection zone is provided with urine Protein Detection test paper, leucocyte Test paper, one or more in fecal occult blood Test paper and nitrite Test paper.
The described urine Protein Detection test paper that adheres to, leucocyte Test paper, the first base plate of fecal occult blood Test paper and nitrite Test paper or the material of the second base plate are the one in PET, PVC, PE, PP or PS, long is 10-300mm, wide 2-30mm, thickness is 0.1-1.5mm.
Described urine Protein Detection test paper, leucocyte Test paper, the basic material of fecal occult blood Test paper and nitrite Test paper is cellulose filter paper, glass fiber filter paper, chromatographic paper, polyester film or nylon membrane; Described test paper be shaped as square, rectangle, circle, ellipse, triangle, rhombus or trapezoidal; Size is long 1-30 mm; Wide 1-30 mm; Thickness 0.01-5 mm; Spacing between multiple test paper is 1-30 mm.
On described pad, there is the immune labeled antibody of interstitialcellstimulating hormone (ICSH) LH.
In described chromatographic film, be coated with interstitialcellstimulating hormone (ICSH) LH and detect antibody.
The beneficial effects of the utility model: the utility model can sample by oneself, primary sample can detect LH and urinary system infection contamination simultaneously, simple and convenient, is applicable to large-scale promotion application.
Accompanying drawing explanation
Fig. 1 is embodiment 1 first base plate 10 structural representations.
Fig. 2 is embodiment 1 second base plate 11 structural representations.
Fig. 3 is embodiment 2 structural representations.
Fig. 4 is embodiment 3 structural representations.
Fig. 5 is embodiment 4 structural representations.
Fig. 6 is embodiment 1 infection index detection zone structural representation.
Fig. 7 is embodiment 2 infection index detection zone structural representations.
Fig. 8 is embodiment 3 infection index detection zone structural representations.
Fig. 9 is embodiment 4 infection index detection zone structural representations.
Embodiment
As shown in Fig. 1,2,5, a kind of interstitialcellstimulating hormone (ICSH) and urinary system infection contamination joint inspection kit, comprise the first base plate 10, is provided with chromatographic film 6 in the one side of the first base plate 10, the front end of chromatographic film 6 arranges opaque diaphragm 3, and the front end of opaque diaphragm 3 arranges arrow 2; The front end of arrow 2 arranges pad 1; In chromatographic film 6, be also disposed with detection line 4 and nature controlling line 5, chromatographic film 6 rear ends are provided with adsorptive pads 7.
Also comprise infection index detection zone 8.Described infection index detection zone 8 is attached to the upper surface of the first base plate 10.
Described infection index detection zone 8 is provided with described urine Protein Detection test paper.Comprise LH immunochromatographydetecting detecting test strip, and urinary system infection contamination index detection zone, comprise described urine Protein Detection test paper, with horizontal coverage mode or built-in embedded mode, be placed in the upper surface of base plate 10.
As shown in Fig. 3,7, a kind of interstitialcellstimulating hormone (ICSH) and urinary system infection contamination joint inspection kit, comprise the first base plate 10, is provided with chromatographic film 6 in the one side of the first base plate 10, the front end of chromatographic film 6 arranges opaque diaphragm 3, and the front end of opaque diaphragm 3 arranges arrow 2; The front end of arrow 2 arranges pad 1; In chromatographic film 6, be also disposed with detection line 4 and nature controlling line 5, chromatographic film 6 rear ends are provided with adsorptive pads 7.
Also comprise infection index detection zone 8.Described infection index detection zone 8 is attached on the second base plate 11.
Described infection index detection zone 8 is provided with leucocyte Test paper, fecal occult blood Test paper.With horizontal coverage mode or built-in embedded mode, be placed in the upper surface of base plate, and be set up in parallel on draw-in groove with LH immunochromatographydetecting detecting test strip.
As shown in Fig. 4,8, a kind of interstitialcellstimulating hormone (ICSH) and urinary system infection contamination joint inspection kit, comprise the first base plate 10, is provided with chromatographic film 6 in the one side of the first base plate 10, and the front end of chromatographic film 6 arranges pad 1, and the front of pad 1 also arranges sample pad 9; In chromatographic film 6, be also disposed with detection line 4 and nature controlling line 5, chromatographic film 6 rear ends are provided with adsorptive pads 7; On the lower surface of the first base plate 10, be also provided with infection index detection zone 8.
Described infection index detection zone 8 is attached on the lower surface of the first base plate 10.
Described infection index detection zone 8 is provided with urine Protein Detection test paper, leucocyte Test paper, fecal occult blood Test paper, is placed in the surface of base plate with horizontal coverage mode or built-in embedded mode, and is placed in respectively the upper and lower surface of base plate with LH immunochromatographydetecting detecting test strip.
As shown in Fig. 5,9, a kind of interstitialcellstimulating hormone (ICSH) and urinary system infection contamination joint inspection kit, comprise the first base plate 10, is provided with chromatographic film 6 in the one side of the first base plate 10, the front end of chromatographic film 6 arranges opaque diaphragm 3, and the front end of opaque diaphragm 3 arranges arrow 2; The front end of arrow 2 arranges pad 1; In chromatographic film 6, be also disposed with detection line 4 and nature controlling line 5, chromatographic film 6 rear ends are provided with adsorptive pads 7.Described infection index detection zone 8 is attached on the second base plate 11.
In embodiment 1-4, the immune labeled antibody of described LH is anti-human LH colloid gold immune labelled antibody, and it is that anti-human LH detects antibody that described LH detects antibody, and anti-human LH colloid gold immune labelled antibody wherein and anti-human LH detect the different parts of antibody in conjunction with people LH.
Described infection index detection zone 8 is provided with urine Protein Detection test paper, leucocyte Test paper, fecal occult blood Test paper and nitrite Test paper, be placed in the upper surface of base plate, and be placed on dismountable draw-in groove with LH immunochromatographydetecting detecting test strip with horizontal coverage mode or built-in embedded mode.
Described urine Protein Detection test paper is in bromophenol blue by blank filter paper, dry processing after soaking in the solution of surfactant and citrate buffer solution composition, the concentration of the bromophenol blue of telling in solution be 100-2000 mg/L, the concentration of surfactant in solution is 0.1-10 %, the pH value of citrate buffer solution is 2.8-4.6, and volumetric molar concentration is 0.01-1 M;
Described leucocyte Test paper is dry processing after blank filter paper is soaked in the solution of the chloro-3-indolyl acetic acid salt of the bromo-4-of 5-and sucrose composition, the concentration of the chloro-3-indolyl acetic acid salt of the bromo-4-of described 5-in solution is 40-1000 mg/L, and the concentration of described sucrose in solution is 200-10000 mg/L;
Described fecal occult blood Test paper is at hydrogen peroxide or dicumyl peroxide by blank filter paper, tetramethyl benzidine or aminopyrine, dry processing after soaking in the solution of surfactant and PBS damping fluid composition, the massfraction of described hydrogen peroxide or dicumyl peroxide is 1-10%, the concentration of tetramethyl benzidine (TMB) or aminopyrine is 0.1-40 g/L, the pH value of PBS damping fluid is 7.0, and volumetric molar concentration is 0.01 ~ 1 M;
Described nitrite Test paper is at sulfanilic acid by blank filter paper, dry processing after soaking in the solution of alpha-naphthylamine and tartrate composition, the concentration of described sulfanilic acid is 500-4000 mg/L, the concentration of alpha-naphthylamine is 500-4000 mg/L, and tartaric massfraction is 0.05-10%.
If band appears in LH immunochromatographydetecting detecting test strip C district during detection, T district occurs that band represents that sample is positive, does not occur that band represents feminine gender; If band does not appear in C district, represent that test strips lost efficacy.Urine Protein Detection test paper color change represent undesired, color do not change represent normal.Leucocyte Test paper does not develop the color and represents normally, occurs that blue or green represents undesired.Fecal occult blood Test paper does not develop the color and represents normally, occurs that blue (or bluish violet) represents undesired.Nitrite Test paper does not develop the color and represents normally, occurs that pink or redness represent undesired.
Preparation Example 1
The antibody colloidal gold labeling method detecting for LH is for adding 200 μ L, and 3% sal tartari regulates the pH of 50 mL colloidal gold solutions; Under stirring condition, add gradually 0.15 mL, 6.6 mg/mL anti-LH antibody to be marked, in above-mentioned colloidal gold solution, mixes standing 30 min of room temperature; 12.5 mL confining liquids are slowly added in above-mentioned colloidal gold solution, mix, standing 30 min of room temperature; The centrifugal 1h of 8000 4 ℃ of g, removes supernatant, stays loose deposits; Each pipe precipitation is dissolved with 25 mL cleansing solutions, and the centrifugal 1h of 6,000 4 ℃ of g, removes supernatant, stays loose deposits; Each pipe precipitation is dissolved with 1.2 mL cleansing solutions, proceeds in 1.5 mL centrifuge tubes, and the centrifugal 1h of 4,500 4 ℃ of g, removes supernatant; All precipitations are resuspended with the resuspended liquid of 1 mL colloidal gold antibody, 4 ℃ of preservations.
The specking method of the antibody colloidal gold pad detecting for LH is as follows: use the resuspended liquid of colloidal gold antibody by 4 times of the colloidal gold antibody bond dilutions of above-mentioned preparation; The power supply of opening point film instrument, sets specking program, and specking amount is 8 μ L/cm; No. 1 pipeline is specking passage; No. 1 pipeline is placed in to the resuspended solution of colloidal gold antibody, selects initialize routine, 6 circulations of initialization; Gold mark pad is lain on a film instrument by fixed position, press " GO " key on control panel and start specking, after having put, take off, check the good gold mark pad of specking, the colloidal gold antibody band of specking evenly, continuously and the straight line that connects whole gold mark pad be qualified specking product, in two straight lines, occur that breakpoint is defective specking product; Often put a slice gold mark pad, " GO " key of pressing on a control panel is once (a slice) of specking; Specking finishes, and the gold mark pad of specking is placed in to room temperature natural drying 1 hour, on film, should can't see specking vestige.
The antibody chromatography membrane preparation method detecting for LH is as follows: get mouse-anti people LH antibody 500 ug, be added in 5 mL graduated centrifuge tubes, antibody diluent to 1 mL, Container Tag T sign.Get sheep anti-mouse igg antibody 25 μ L, be added in 5 mL graduated centrifuge tubes, antibody diluent to 1 mL, Container Tag C sign; The power supply of opening point film instrument, sets specking program, and specking amount is 1 μ L/cm, and No. 1 pipeline is for detecting band specking passage, and No. 2 pipelines are contrast band specking passage; No. 1 pipeline is placed in and detects band solution, No. 2 pipelines are placed in to contrast band solution, select initialize routine, 6 circulations of initialization; Chromatographic film is lain on a film instrument by fixed position, press " GO " key on control panel and start specking, after having put, take off, check the good chromatographic film of specking, detect band and contrast band and be two evenly, continuously and the straight line that connects whole chromatographic film be qualified specking product, in two straight lines, occur that breakpoint is defective specking product; Often put a slice chromatographic film, " GO " key of pressing on a control panel is once (a slice) of specking; Specking finishes, and the chromatographic film of specking is placed in to room temperature natural drying 1 hour, on film, should can't see specking vestige.
The protection sheet of wider portion on base plate is removed, and the edge coboundary of protection sheet above, will pull the chromatographic film of line, in C line mode up, is attached on base plate plate; Collaurum pad is attached to T line below, and a little contacts with NC film; Sample pad is attached to collaurum pad below, and a little contacts with collaurum pad; Then remove top protection sheet, adsorptive pads is attached to the top of NC film, a little contacts with NC film; Protection sheet and index strip paper are attached to the test strips outside assembling one by one, are assembled into kilocalorie.Connect cutting machine power supply, set the film program of cutting, setting cutting width is 4 mm; Kilocalorie certified products are kept flat in cutting machine platform track, face up, press " GO " key on guidance panel, start cutting; Often put a slice kilocalorie certified products, press on guidance panel " GO " key once, until cut all kilocalorie certified products.
Described urine Protein Detection test paper is in bromophenol blue by blank filter paper, dry processing after soaking in the solution of surfactant and citrate buffer solution composition, the concentration of the bromophenol blue of telling in solution be 500 mg/L, the concentration of surfactant in solution is 10 %, the pH value of citrate buffer solution is 4.6, volumetric molar concentration is 0.01 M, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm;
Described leucocyte Test paper is dry processing after blank filter paper is soaked in the solution of the chloro-3-indolyl acetic acid salt of the bromo-4-of 5-and sucrose composition, the concentration of the chloro-3-indolyl acetic acid salt of the bromo-4-of described 5-in solution is 800 mg/L, the concentration of described sucrose in solution is 10000 mg/L, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm;
Described fecal occult blood Test paper is at hydrogen peroxide or dicumyl peroxide by blank filter paper, tetramethyl benzidine or aminopyrine, dry processing after soaking in the solution of surfactant and PBS damping fluid composition, the massfraction of described hydrogen peroxide or dicumyl peroxide is 10%, the concentration of tetramethyl benzidine (TMB) or aminopyrine is 40 g/L, the pH value of PBS damping fluid is 7.0, and volumetric molar concentration is 0.01 M, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm;
Described nitrite Test paper is at sulfanilic acid by blank filter paper, dry processing after soaking in the solution of alpha-naphthylamine and tartrate composition, the concentration of described sulfanilic acid is 4000 mg/L, the concentration of alpha-naphthylamine is 2000 mg/L, tartaric massfraction is 0.05%, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm.
To urinate respectively Protein Detection test paper, leucocyte Test paper, one or more in fecal occult blood Test paper and nitrite Test paper cover with level or built-in embedded mode is pasted and is fixed on transparent PVC sheet, each test paper spacing is 5 mm, are cut into the length of side and are the test strips of 4 mm.
Preparation Example 2
The antibody colloidal gold labeling method detecting for LH is for adding 200 μ L, and 3% sal tartari regulates the pH of 50 mL colloidal gold solutions; Under stirring condition, add gradually 0.15 mL, 6.6 mg/mL anti-LH antibody to be marked, in above-mentioned colloidal gold solution, mixes standing 30 min of room temperature; 12.5 mL confining liquids are slowly added in above-mentioned colloidal gold solution, mix, standing 30 min of room temperature; The centrifugal 1h of 8000 4 ℃ of g, removes supernatant, stays loose deposits; Each pipe precipitation is dissolved with 25 mL cleansing solutions, and the centrifugal 1h of 6,000 4 ℃ of g, removes supernatant, stays loose deposits; Each pipe precipitation is dissolved with 1.2 mL cleansing solutions, proceeds in 1.5 mL centrifuge tubes, and the centrifugal 1h of 4,500 4 ℃ of g, removes supernatant; All precipitations are resuspended with the resuspended liquid of 1 mL colloidal gold antibody, 4 ℃ of preservations.
The specking method of the antibody colloidal gold pad detecting for LH is as follows: use the resuspended liquid of colloidal gold antibody by 4 times of the colloidal gold antibody bond dilutions of above-mentioned preparation; The power supply of opening point film instrument, sets specking program, and specking amount is 8 μ L/cm; No. 1 pipeline is specking passage; No. 1 pipeline is placed in to the resuspended solution of colloidal gold antibody, selects initialize routine, 6 circulations of initialization; Gold mark pad is lain on a film instrument by fixed position, press " GO " key on control panel and start specking, after having put, take off, check the good gold mark pad of specking, the colloidal gold antibody band of specking evenly, continuously and the straight line that connects whole gold mark pad be qualified specking product, in two straight lines, occur that breakpoint is defective specking product; Often put a slice gold mark pad, " GO " key of pressing on a control panel is once (a slice) of specking; Specking finishes, and the gold mark pad of specking is placed in to room temperature natural drying 1 hour, on film, should can't see specking vestige.
The antibody chromatography membrane preparation method detecting for LH is as follows: get mouse-anti people LH antibody 500 ug, be added in 5 mL graduated centrifuge tubes, antibody diluent to 1 mL, Container Tag T sign.Get sheep anti-mouse igg antibody 25 μ L, be added in 5 mL graduated centrifuge tubes, antibody diluent to 1 mL, Container Tag C sign; The power supply of opening point film instrument, sets specking program, and specking amount is 1 μ L/cm, and No. 1 pipeline is for detecting band specking passage, and No. 2 pipelines are contrast band specking passage; No. 1 pipeline is placed in and detects band solution, No. 2 pipelines are placed in to contrast band solution, select initialize routine, 6 circulations of initialization; Chromatographic film is lain on a film instrument by fixed position, press " GO " key on control panel and start specking, after having put, take off, check the good chromatographic film of specking, detect band and contrast band and be two evenly, continuously and the straight line that connects whole chromatographic film be qualified specking product, in two straight lines, occur that breakpoint is defective specking product; Often put a slice chromatographic film, " GO " key of pressing on a control panel is once (a slice) of specking; Specking finishes, and the chromatographic film of specking is placed in to room temperature natural drying 1 hour, on film, should can't see specking vestige.
The protection sheet of wider portion on base plate is removed, and the edge coboundary of protection sheet above, will pull the chromatographic film of line, in C line mode up, is attached on base plate plate; Collaurum pad is attached to T line below, and a little contacts with NC film; Sample pad is attached to collaurum pad below, and a little contacts with collaurum pad; Then remove top protection sheet, adsorptive pads is attached to the top of NC film, a little contacts with NC film; Protection sheet and index strip paper are attached to the test strips outside assembling one by one, are assembled into kilocalorie.Connect cutting machine power supply, set the film program of cutting, setting cutting width is 4 mm; Kilocalorie certified products are kept flat in cutting machine platform track, face up, press " GO " key on guidance panel, start cutting; Often put a slice kilocalorie certified products, press on guidance panel " GO " key once, until cut all kilocalorie certified products.
Described urine Protein Detection test paper is in bromophenol blue by blank filter paper, dry processing after soaking in the solution of surfactant and citrate buffer solution composition, the concentration of the bromophenol blue of telling in solution be 500 mg/L, the concentration of surfactant in solution is 10 %, the pH value of citrate buffer solution is 4.6, volumetric molar concentration is 0.01 M, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm;
Described leucocyte Test paper is dry processing after blank filter paper is soaked in the solution of the chloro-3-indolyl acetic acid salt of the bromo-4-of 5-and sucrose composition, the concentration of the chloro-3-indolyl acetic acid salt of the bromo-4-of described 5-in solution is 800 mg/L, the concentration of described sucrose in solution is 10000 mg/L, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm;
Described fecal occult blood Test paper is at hydrogen peroxide or dicumyl peroxide by blank filter paper, tetramethyl benzidine or aminopyrine, dry processing after soaking in the solution of surfactant and PBS damping fluid composition, the massfraction of described hydrogen peroxide or dicumyl peroxide is 10%, the concentration of tetramethyl benzidine (TMB) or aminopyrine is 40 g/L, the pH value of PBS damping fluid is 7.0, and volumetric molar concentration is 0.01 M, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm;
Described nitrite Test paper is at sulfanilic acid by blank filter paper, dry processing after soaking in the solution of alpha-naphthylamine and tartrate composition, the concentration of described sulfanilic acid is 4000 mg/L, the concentration of alpha-naphthylamine is 2000 mg/L, tartaric massfraction is 0.05%, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm.
To urinate respectively Protein Detection test paper, leucocyte Test paper, one or more in fecal occult blood Test paper and nitrite Test paper cover with level or the stickup of built-in embedded mode is fixed on transparent PVC sheet, each test paper spacing is 5 mm, be cut into the length of side and be the test strips of 4 mm, and be placed in side by side on draw-in groove with LH immunochromatographydetecting detecting test strip.
Preparation Example 3
The antibody colloidal gold labeling method detecting for LH is for adding 200 μ L, and 3% sal tartari regulates the pH of 50 mL colloidal gold solutions; Under stirring condition, add gradually 0.15 mL, 6.6 mg/mL anti-LH antibody to be marked, in above-mentioned colloidal gold solution, mixes standing 30 min of room temperature; 12.5 mL confining liquids are slowly added in above-mentioned colloidal gold solution, mix, standing 30 min of room temperature; The centrifugal 1h of 8000 4 ℃ of g, removes supernatant, stays loose deposits; Each pipe precipitation is dissolved with 25 mL cleansing solutions, and the centrifugal 1h of 6,000 4 ℃ of g, removes supernatant, stays loose deposits; Each pipe precipitation is dissolved with 1.2 mL cleansing solutions, proceeds in 1.5 mL centrifuge tubes, and the centrifugal 1h of 4,500 4 ℃ of g, removes supernatant; All precipitations are resuspended with the resuspended liquid of 1 mL colloidal gold antibody, 4 ℃ of preservations.
The specking method of the antibody colloidal gold pad detecting for LH is as follows: use the resuspended liquid of colloidal gold antibody by 4 times of the colloidal gold antibody bond dilutions of above-mentioned preparation; The power supply of opening point film instrument, sets specking program, and specking amount is 8 μ L/cm; No. 1 pipeline is specking passage; No. 1 pipeline is placed in to the resuspended solution of colloidal gold antibody, selects initialize routine, 6 circulations of initialization; Gold mark pad is lain on a film instrument by fixed position, press " GO " key on control panel and start specking, after having put, take off, check the good gold mark pad of specking, the colloidal gold antibody band of specking evenly, continuously and the straight line that connects whole gold mark pad be qualified specking product, in two straight lines, occur that breakpoint is defective specking product; Often put a slice gold mark pad, " GO " key of pressing on a control panel is once (a slice) of specking; Specking finishes, and the gold mark pad of specking is placed in to room temperature natural drying 1 hour, on film, should can't see specking vestige.
The antibody chromatography membrane preparation method detecting for LH is as follows: get mouse-anti people LH antibody 500 ug, be added in 5 mL graduated centrifuge tubes, antibody diluent to 1 mL, Container Tag T sign.Get sheep anti-mouse igg antibody 25 μ L, be added in 5 mL graduated centrifuge tubes, antibody diluent to 1 mL, Container Tag C sign; The power supply of opening point film instrument, sets specking program, and specking amount is 1 μ L/cm, and No. 1 pipeline is for detecting band specking passage, and No. 2 pipelines are contrast band specking passage; No. 1 pipeline is placed in and detects band solution, No. 2 pipelines are placed in to contrast band solution, select initialize routine, 6 circulations of initialization; Chromatographic film is lain on a film instrument by fixed position, press " GO " key on control panel and start specking, after having put, take off, check the good chromatographic film of specking, detect band and contrast band and be two evenly, continuously and the straight line that connects whole chromatographic film be qualified specking product, in two straight lines, occur that breakpoint is defective specking product; Often put a slice chromatographic film, " GO " key of pressing on a control panel is once (a slice) of specking; Specking finishes, and the chromatographic film of specking is placed in to room temperature natural drying 1 hour, on film, should can't see specking vestige.
The protection sheet of wider portion on base plate is removed, and the edge coboundary of protection sheet above, will pull the chromatographic film of line, in C line mode up, is attached on base plate plate; Collaurum pad is attached to T line below, and a little contacts with NC film; Sample pad is attached to collaurum pad below, and a little contacts with collaurum pad; Then remove top protection sheet, adsorptive pads is attached to the top of NC film, a little contacts with NC film; Protection sheet and index strip paper are attached to the test strips outside assembling one by one, are assembled into kilocalorie.Connect cutting machine power supply, set and cut film program, setting cutting width is 4mm; Kilocalorie certified products are kept flat in cutting machine platform track, face up, press " GO " key on guidance panel, start cutting; Often put a slice kilocalorie certified products, press on guidance panel " GO " key once, until cut all kilocalorie certified products.
Described urine Protein Detection test paper is in bromophenol blue by blank filter paper, dry processing after soaking in the solution of surfactant and citrate buffer solution composition, the concentration of the bromophenol blue of telling in solution be 500 mg/L, the concentration of surfactant in solution is 10 %, the pH value of citrate buffer solution is 4.6, volumetric molar concentration is 0.01 M, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm;
Described leucocyte Test paper is dry processing after blank filter paper is soaked in the solution of the chloro-3-indolyl acetic acid salt of the bromo-4-of 5-and sucrose composition, the concentration of the chloro-3-indolyl acetic acid salt of the bromo-4-of described 5-in solution is 800 mg/L, the concentration of described sucrose in solution is 10000 mg/L, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm;
Described fecal occult blood Test paper is at hydrogen peroxide or dicumyl peroxide by blank filter paper, tetramethyl benzidine or aminopyrine, dry processing after soaking in the solution of surfactant and PBS damping fluid composition, the massfraction of described hydrogen peroxide or dicumyl peroxide is 10%, the concentration of tetramethyl benzidine (TMB) or aminopyrine is 40 g/L, the pH value of PBS damping fluid is 7.0, and volumetric molar concentration is 0.01 M, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm;
Described nitrite Test paper is at sulfanilic acid by blank filter paper, dry processing after soaking in the solution of alpha-naphthylamine and tartrate composition, the concentration of described sulfanilic acid is 4000 mg/L, the concentration of alpha-naphthylamine is 2000 mg/L, tartaric massfraction is 0.05%, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm.
To urinate respectively Protein Detection test paper, leucocyte Test paper, one or more in fecal occult blood Test paper and nitrite Test paper cover with level or the stickup of built-in embedded mode is fixed on transparent PVC sheet, each test paper spacing is 5 mm, be cut into the length of side and be the test strips of 4 mm, and be placed in side by side with HCG immunochromatographydetecting detecting test strip the upper and lower surface that is placed in respectively base plate on draw-in groove.
Preparation Example 4
The antibody colloidal gold labeling method detecting for LH is for adding 200 μ L, and 3% sal tartari regulates the pH of 50 mL colloidal gold solutions; Under stirring condition, add gradually 0.15 mL, 6.6 mg/mL anti-LH antibody to be marked, in above-mentioned colloidal gold solution, mixes standing 30 min of room temperature; 12.5 mL confining liquids are slowly added in above-mentioned colloidal gold solution, mix, standing 30 min of room temperature; The centrifugal 1h of 8000 4 ℃ of g, removes supernatant, stays loose deposits; Each pipe precipitation is dissolved with 25 mL cleansing solutions, and the centrifugal 1h of 6,000 4 ℃ of g, removes supernatant, stays loose deposits; Each pipe precipitation is dissolved with 1.2 mL cleansing solutions, proceeds in 1.5 mL centrifuge tubes, and the centrifugal 1h of 4,500 4 ℃ of g, removes supernatant; All precipitations are resuspended with the resuspended liquid of 1 mL colloidal gold antibody, 4 ℃ of preservations.
The specking method of the antibody colloidal gold pad detecting for LH is as follows: use the resuspended liquid of colloidal gold antibody by 4 times of the colloidal gold antibody bond dilutions of above-mentioned preparation; The power supply of opening point film instrument, sets specking program, and specking amount is 8 μ L/cm; No. 1 pipeline is specking passage; No. 1 pipeline is placed in to the resuspended solution of colloidal gold antibody, selects initialize routine, 6 circulations of initialization; Gold mark pad is lain on a film instrument by fixed position, press " GO " key on control panel and start specking, after having put, take off, check the good gold mark pad of specking, the colloidal gold antibody band of specking evenly, continuously and the straight line that connects whole gold mark pad be qualified specking product, in two straight lines, occur that breakpoint is defective specking product; Often put a slice gold mark pad, " GO " key of pressing on a control panel is once (a slice) of specking; Specking finishes, and the gold mark pad of specking is placed in to room temperature natural drying 1 hour, on film, should can't see specking vestige.
The antibody chromatography membrane preparation method detecting for LH is as follows: get mouse-anti people LH antibody 500 ug, be added in 5 mL graduated centrifuge tubes, antibody diluent to 1 mL, Container Tag T sign.Get sheep anti-mouse igg antibody 25 μ L, be added in 5 mL graduated centrifuge tubes, antibody diluent to 1 mL, Container Tag C sign; The power supply of opening point film instrument, sets specking program, and specking amount is 1 μ L/cm, and No. 1 pipeline is for detecting band specking passage, and No. 2 pipelines are contrast band specking passage; No. 1 pipeline is placed in and detects band solution, No. 2 pipelines are placed in to contrast band solution, select initialize routine, 6 circulations of initialization; Chromatographic film is lain on a film instrument by fixed position, press " GO " key on control panel and start specking, after having put, take off, check the good chromatographic film of specking, detect band and contrast band and be two evenly, continuously and the straight line that connects whole chromatographic film be qualified specking product, in two straight lines, occur that breakpoint is defective specking product; Often put a slice chromatographic film, " GO " key of pressing on a control panel is once (a slice) of specking; Specking finishes, and the chromatographic film of specking is placed in to room temperature natural drying 1 hour, on film, should can't see specking vestige.
The protection sheet of wider portion on base plate is removed, and the edge coboundary of protection sheet above, will pull the chromatographic film of line, in C line mode up, is attached on base plate plate; Collaurum pad is attached to T line below, and a little contacts with NC film; Sample pad is attached to collaurum pad below, and a little contacts with collaurum pad; Then remove top protection sheet, adsorptive pads is attached to the top of NC film, a little contacts with NC film; Protection sheet and index strip paper are attached to the test strips outside assembling one by one, are assembled into kilocalorie.Connect cutting machine power supply, set the film program of cutting, setting cutting width is 4 mm; Kilocalorie certified products are kept flat in cutting machine platform track, face up, press " GO " key on guidance panel, start cutting; Often put a slice kilocalorie certified products, press on guidance panel " GO " key once, until cut all kilocalorie certified products.
Described urine Protein Detection test paper is in bromophenol blue by blank filter paper, dry processing after soaking in the solution of surfactant and citrate buffer solution composition, the concentration of the bromophenol blue of telling in solution be 500 mg/L, the concentration of surfactant in solution is 10 %, the pH value of citrate buffer solution is 4.6, volumetric molar concentration is 0.01 M, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm;
Described leucocyte Test paper is dry processing after blank filter paper is soaked in the solution of the chloro-3-indolyl acetic acid salt of the bromo-4-of 5-and sucrose composition, the concentration of the chloro-3-indolyl acetic acid salt of the bromo-4-of described 5-in solution is 800 mg/L, the concentration of described sucrose in solution is 10000 mg/L, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm;
Described fecal occult blood Test paper is at hydrogen peroxide or dicumyl peroxide by blank filter paper, tetramethyl benzidine or aminopyrine, dry processing after soaking in the solution of surfactant and PBS damping fluid composition, the massfraction of described hydrogen peroxide or dicumyl peroxide is 10%, the concentration of tetramethyl benzidine (TMB) or aminopyrine is 40 g/L, the pH value of PBS damping fluid is 7.0, and volumetric molar concentration is 0.01 M, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm;
Described nitrite Test paper is at sulfanilic acid by blank filter paper, dry processing after soaking in the solution of alpha-naphthylamine and tartrate composition, the concentration of described sulfanilic acid is 4000 mg/L, the concentration of alpha-naphthylamine is 2000 mg/L, tartaric massfraction is 0.05%, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm.
To urinate respectively Protein Detection test paper, leucocyte Test paper, one or more in fecal occult blood Test paper and nitrite Test paper cover with level or the stickup of built-in embedded mode is fixed on transparent PVC sheet, each test paper spacing is 5 mm, be cut into the length of side and be the test strips of 4 mm, and be placed in side by side on dismountable draw-in groove with HCG immunochromatographydetecting detecting test strip.
Claims (4)
1. an interstitialcellstimulating hormone (ICSH) and urinary system infection contamination joint inspection kit, comprise the first base plate (10), it is characterized in that: in the one side of the first base plate (10), be provided with chromatographic film (6), the front end of chromatographic film (6) arranges opaque diaphragm (3), and the front end of opaque diaphragm (3) arranges arrow (2); The front end of arrow (2) arranges pad (1); In chromatographic film (6), be also disposed with detection line (4) and nature controlling line (5), chromatographic film (6) rear end is provided with adsorptive pads (7).
2. interstitialcellstimulating hormone (ICSH) and urinary system infection contamination joint inspection kit as claimed in claim 1, is characterized in that: also comprise infection index detection zone (8).
3. interstitialcellstimulating hormone (ICSH) and urinary system infection contamination joint inspection kit as claimed in claim 2, is characterized in that: it is upper or be compound on any one surface of the first base plate (10) that described infection index detection zone (8) is attached to the second base plate (11).
4. an interstitialcellstimulating hormone (ICSH) and urinary system infection contamination joint inspection kit, comprise the first base plate (10), it is characterized in that: in the one side of the first base plate (10), be provided with chromatographic film (6), the front end of chromatographic film (6) arranges pad (1), and the front of pad (1) also arranges sample pad (9); In chromatographic film (6), be also disposed with detection line (4) and nature controlling line (5), chromatographic film (6) rear end is provided with adsorptive pads (7); On the lower surface of the first base plate (10), be also provided with infection index detection zone (8).
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN103529206A (en) * | 2013-11-04 | 2014-01-22 | 无锡博慧斯生物医药科技有限公司 | Luteinizing hormone and bacterial vaginosis joint-test kit |
CN103543259A (en) * | 2013-11-04 | 2014-01-29 | 无锡博慧斯生物医药科技有限公司 | Luteinizing hormone and urinary system infection combined assay kit |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN103529206A (en) * | 2013-11-04 | 2014-01-22 | 无锡博慧斯生物医药科技有限公司 | Luteinizing hormone and bacterial vaginosis joint-test kit |
CN103543259A (en) * | 2013-11-04 | 2014-01-29 | 无锡博慧斯生物医药科技有限公司 | Luteinizing hormone and urinary system infection combined assay kit |
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