CN203101395U - Rotavirus, adenovirus and occult blood joint inspection kit - Google Patents
Rotavirus, adenovirus and occult blood joint inspection kit Download PDFInfo
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- CN203101395U CN203101395U CN 201320052873 CN201320052873U CN203101395U CN 203101395 U CN203101395 U CN 203101395U CN 201320052873 CN201320052873 CN 201320052873 CN 201320052873 U CN201320052873 U CN 201320052873U CN 203101395 U CN203101395 U CN 203101395U
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Abstract
The utility model relates to a rotavirus, adenovirus and occult blood joint inspection kit. The rotavirus, adenovirus and occult blood joint inspection kit comprises a rotavirus, adenovirus and occult blood immunochromatographic test strip, or comprises a rotavirus immunochromatographic test strip, an adenovirus immunochromatographic test strip and a occult blood immunochromatographic test strip, or comprises the rotavirus immunochromatographic test strip and an adenovirus and occult blood immunochromatographic test strip, or comprises a rotavirus and adenovirus immunochromatographic test strip and the occult blood immunochromatographic test strip, or comprises a rotavirus and occult blood immunochromatographic test strip and the adenovirus immunochromatographic test strip, and preferably, a corresponding immunolabeled antibody and a corresponding detection antibody are respectively coated on a joint pad and a chromatography membrane of each immunochromatographic test strip. The rotavirus, adenovirus and occult blood joint inspection kit disclosed by the utility model can simultaneously detect rotavirus, adenovirus and occult blood by sampling once, has the advantages of simplicity and convenience in application and capability of providing a strong basis for next-step accurate treatment through joint inspection results and is suitable for large-scale popularization and application.
Description
Technical field
The utility model relates to the detection kit technical field, is specifically related to joint inspection kit technical field, particularly a kind of rotavirus, adenovirus and the joint inspection kit of occulting blood.
Background technology
The infectious diarrhea disease is one group of also popular enteric infection disease all over the world that extensively distributes, its incidence of disease height, propagated fast, especially serious to children's health hazard, wherein in the majority with bacterial diarrhea and virus (mainly comprising rotavirus and adenovirus) infectious diarrhea.
To infection of children diarrhoea, generally to carry out antidiastole clinically, so that make corresponding treatment to pathogen.Detection means commonly used at present is as stool for routine, cultivation and immunology detection; Stool for routine generally comprises color, form, the ight soil cell of ight soil, index such as occult blood, and cultivation mainly is at some bacterial infections; Immunology detection then mainly is indexs such as rotavirus, adenovirus.
In clinical practice, rotavirus, adenovirus and three indexs of occulting blood are great to the significance of differential diagnosis of children's diarrhae, and it is by due to these two kinds of virus infectionses that the rotavirus and the adenovirus positive have been represented diarrhoea, and general symptom is lighter, the course of disease is shorter, and is less to children's harm; The positive of occulting blood then illustrates it is hemorrhagic enteritis, and the course of disease is long, and harm is big.Significance of differential diagnosis to children's diarrhae is great.
Widespread use collaurum or latex agglutination be to rotavirus, adenovirus with occult blood and carry out fast detecting clinically, and general only the need can be diagnosed the pathogen of children's diarrhae in about 10 minutes.At present existing rotavirus, adenovirus and the colloidal gold strip of occulting blood, perhaps the colloidal gold strip of rotavirus, adenovirus joint inspection is very easy to clinical diagnosis; But do not find rotavirus, adenovirus as yet and the product or the report of three the index joint inspections of colloidal gold strip of occulting blood.
Rotavirus, adenovirus and the colloidal gold strip primary sample of occulting blood if can be detected, will make things convenient for the reviewer biglyyer, increase work efficiency.
In order to solve the problems referred to above of existence, a kind of kit need be provided, it can diagnose rotavirus, adenovirus simultaneously and occult blood by primary sample, and is easy to use.
The utility model content
The purpose of this utility model is to have overcome above-mentioned shortcoming of the prior art, provide a kind of rotavirus, adenovirus and the joint inspection kit of occulting blood, this rotavirus, adenovirus and the joint inspection kit of occulting blood can be diagnosed rotavirus, adenovirus simultaneously and occult blood by primary sample, use easy, the joint inspection result accurately treats for next step strong foundation is provided, and is suitable for large-scale promotion application.
To achieve these goals, rotavirus of the present utility model, adenovirus and the joint inspection kit of occulting blood have following formation:
This rotavirus, the adenovirus and the joint inspection kit of occulting blood, be characterized in, comprise rotavirus, the adenovirus and the immunochromatographydetecting detecting test strip of occulting blood, perhaps comprise the rotavirus immunochromatographydetecting detecting test strip, the adenovirus immunochromatographydetecting detecting test strip and the immunochromatographydetecting detecting test strip of occulting blood, perhaps comprise rotavirus immunochromatographydetecting detecting test strip and adenovirus and the immunochromatographydetecting detecting test strip of occulting blood, perhaps comprise rotavirus and adenovirus immunochromatographydetecting detecting test strip and the immunochromatographydetecting detecting test strip of occulting blood, perhaps comprise rotavirus and occult blood immunochromatographydetecting detecting test strip and adenovirus immunochromatographydetecting detecting test strip.
Preferably, have the immune labeled antibody of rotavirus, the immune labeled antibody of adenovirus and the immune labeled antibody of occulting blood on the pad of described rotavirus, adenovirus and the immunochromatographydetecting detecting test strip of occulting blood, be coated with rotavirus on the chromatographic film of described rotavirus, adenovirus and the immunochromatographydetecting detecting test strip of occulting blood and detect antibody, adenovirus detection antibody and the detection antibody of occulting blood.
Preferably, has the immune labeled antibody of rotavirus on the pad of described rotavirus immunochromatographydetecting detecting test strip, be coated with rotavirus on the chromatographic film of described rotavirus immunochromatographydetecting detecting test strip and detect antibody, has the immune labeled antibody of adenovirus on the pad of described adenovirus immunochromatographydetecting detecting test strip, be coated with adenovirus on the chromatographic film of described adenovirus immunochromatographydetecting detecting test strip and detect antibody, have the immune labeled antibody of occulting blood on the pad of the described immunochromatographydetecting detecting test strip of occulting blood, be coated with the detection antibody of occulting blood on the chromatographic film of the described immunochromatographydetecting detecting test strip of occulting blood.
Preferably, has the immune labeled antibody of rotavirus on the pad of described rotavirus immunochromatographydetecting detecting test strip, be coated with rotavirus on the chromatographic film of described rotavirus immunochromatographydetecting detecting test strip and detect antibody, have the immune labeled antibody of adenovirus on the pad of the described adenovirus and the immunochromatographydetecting detecting test strip of occulting blood and the immune labeled antibody of occulting blood, be coated with adenovirus on the chromatographic film of the described adenovirus and the immunochromatographydetecting detecting test strip of occulting blood and detect the antibody and the detection antibody of occulting blood.
Preferably, has immune labeled antibody of rotavirus and the immune labeled antibody of adenovirus on the pad of described rotavirus and adenovirus immunochromatographydetecting detecting test strip, be coated with rotavirus on the chromatographic film of described rotavirus and adenovirus immunochromatographydetecting detecting test strip and detect antibody and adenovirus detection antibody, have the immune labeled antibody of occulting blood on the pad of the described immunochromatographydetecting detecting test strip of occulting blood, be coated with the detection antibody of occulting blood on the chromatographic film of the described immunochromatographydetecting detecting test strip of occulting blood.
Preferably, has the immune labeled antibody of rotavirus on the pad of the described rotavirus and the immunochromatographydetecting detecting test strip of occulting blood and the immune labeled antibody of occulting blood, be coated with rotavirus on the chromatographic film of the described rotavirus and the immunochromatographydetecting detecting test strip of occulting blood and detect the antibody and the detection antibody of occulting blood, have the immune labeled antibody of adenovirus on the pad of described adenovirus immunochromatographydetecting detecting test strip, be coated with adenovirus on the chromatographic film of described adenovirus immunochromatographydetecting detecting test strip and detect antibody.
More preferably, the immune labeled antibody of described rotavirus is the immune labeled antibody of anti-HRV, it is anti-HRV's antibody that described rotavirus detects antibody, the immune labeled antibody of described adenovirus is the immune labeled antibody of anti-adenovirus hominis, it is anti-adenovirus hominis antibody that described adenovirus detects antibody, the described immune labeled antibody of occulting blood is the immune labeled antibody of antihuman hemoglobin or the immune labeled antibody of anti-human transferrin or immune labeled antibody of anti-human erythrocyte matrix or the immune labeled antibody of anti-human albumin, and the described detection antibody of occulting blood is the immune labeled antibody of antihuman hemoglobin or the immune labeled antibody of anti-human transferrin or immune labeled antibody of anti-human erythrocyte matrix or the immune labeled antibody of anti-human albumin.
More preferably, the immune labeled antibody of described rotavirus is rotavirus colloid gold label antibody or rotavirus latex labelled antibody, the immune labeled antibody of described adenovirus is adenovirus colloid gold label antibody or adenovirus latex labelled antibody, and the described immune labeled antibody of occulting blood is occult blood the colloid gold label antibody or the latex labelled antibody of occulting blood.
Preferably, described rotavirus immunochromatographydetecting detecting test strip, described adenovirus immunochromatographydetecting detecting test strip and the described immunochromatographydetecting detecting test strip of occulting blood are set up in parallel.
Preferably, described rotavirus, adenovirus and the joint inspection kit of occulting blood also comprise base plate, and described rotavirus immunochromatographydetecting detecting test strip, described adenovirus immunochromatographydetecting detecting test strip and the described immunochromatographydetecting detecting test strip of occulting blood are fitted in respectively on the upper and lower surface of described base plate.
The beneficial effects of the utility model are:
(1) the utility model comprises rotavirus, the adenovirus and the immunochromatographydetecting detecting test strip of occulting blood, perhaps comprise the rotavirus immunochromatographydetecting detecting test strip, the adenovirus immunochromatographydetecting detecting test strip and the immunochromatographydetecting detecting test strip of occulting blood, perhaps comprise rotavirus immunochromatographydetecting detecting test strip and adenovirus and the immunochromatographydetecting detecting test strip of occulting blood, perhaps comprise rotavirus and adenovirus immunochromatographydetecting detecting test strip and the immunochromatographydetecting detecting test strip of occulting blood, perhaps comprise rotavirus and occult blood immunochromatographydetecting detecting test strip and adenovirus immunochromatographydetecting detecting test strip, thereby primary sample can be to rotavirus, adenovirus detects with occulting blood; Avoided repeatedly sampling operation testing result loaded down with trivial details and that the sampling inequality causes inaccurate.
(2) detect rotavirus, adenovirus simultaneously and three indexs of occulting blood are very necessary, by use of the present utility model, to the children's diarrhae due to rotavirus and the adenovirus, because of symptom lighter, the course of disease is shorter, and is less to children's harm, gets final product so only carry out general treatment; The positive of occulting blood then illustrates it is hemorrhagic enteritis, and the course of disease is long, and harm is big, need carry out in time and effectively treatment.
Therefore, the utility model can be diagnosed rotavirus, adenovirus simultaneously and occults blood by primary sample, use easy, the joint inspection result for next step accurately treatment strong foundation is provided, be suitable for large-scale promotion application.
Description of drawings
Fig. 1 is that the master of a specific embodiment of the present utility model looks synoptic diagram.Wherein 1 is pad (containing anti-HRV's colloid gold label antibody, anti-adenovirus hominis colloid gold label antibody and antihuman hemoglobin colloid gold label antibody); The 2nd, arrow; The 3rd, the opaque diaphragm of part; 4 is first detection line (bag is by anti-HRV's antibody); 5 is second detection line (bag is by anti-adenovirus hominis antibody); 6 is the 3rd detection line (bag is by antihuman hemoglobin antibody); The 7th, nature controlling line; The 8th, chromatographic film; The 9th, adsorptive pads.
Fig. 2 is that the master of another specific embodiment of the present utility model looks synoptic diagram.Wherein, the 9th, pad (containing antihuman hemoglobin); The 10th, pad (containing anti-HRV's colloid gold label antibody, anti-adenovirus hominis colloid gold label antibody); The 2nd, arrow; The 3rd, the opaque diaphragm of part; 4 is first detection line (bag is by anti-HRV's antibody); 5 is second detection line (bag is by anti-adenovirus hominis antibody); 6 is the 3rd detection line (bag is by antihuman hemoglobin antibody); The 7th, nature controlling line; The 8th, chromatographic film; The 9th, adsorptive pads, the 14th, base plate.
Fig. 3 is that the master of another specific embodiment of the present utility model looks synoptic diagram.Wherein 9 is pad (containing antihuman hemoglobin colloid gold label antibody); The 12nd, pad (containing anti-HRV's colloid gold label antibody); The 13rd, pad (containing anti-adenovirus hominis colloid gold label antibody); The 2nd, arrow; The 3rd, the opaque diaphragm of part; 4 is first detection line (bag is by anti-HRV's antibody); 5 is second detection line (bag is by anti-adenovirus hominis antibody); 6 is the 3rd detection line (bag is by antihuman hemoglobin antibody); The 7th, nature controlling line; The 8th, chromatographic film; The 9th, adsorptive pads, the 14th, base plate.
Fig. 4 is that the master of a specific embodiment more of the present utility model looks synoptic diagram.Wherein, wherein, the 9th, pad (containing antihuman hemoglobin); The 10th, pad (containing anti-HRV's colloid gold label antibody, anti-adenovirus hominis colloid gold label antibody); The 2nd, arrow; The 3rd, the opaque diaphragm of part; 4 is first detection line (bag is by anti-HRV's antibody); 5 is second detection line (bag is by anti-adenovirus hominis antibody); 6 is the 3rd detection line (bag is by antihuman hemoglobin antibody); The 7th, nature controlling line; The 8th, chromatographic film; The 9th, adsorptive pads, the 14th, base plate.
Embodiment
In order more to be expressly understood technology contents of the present utility model, describe in detail especially exemplified by following examples.What wherein Fig. 1 showed is a specific embodiment of the present utility model, and what Fig. 2 showed is another specific embodiment of the present utility model, and what Fig. 3 showed is another specific embodiment of the present utility model, and what Fig. 4 showed is a specific embodiment more of the present utility model.Wherein identical parts adopt identical Reference numeral.
See also Fig. 1-shown in Figure 4, rotavirus of the present utility model, the adenovirus and the joint inspection kit of occulting blood comprise rotavirus, the adenovirus and the immunochromatographydetecting detecting test strip of occulting blood, perhaps comprise the rotavirus immunochromatographydetecting detecting test strip, the adenovirus immunochromatographydetecting detecting test strip and the immunochromatographydetecting detecting test strip of occulting blood, perhaps comprise rotavirus immunochromatographydetecting detecting test strip and adenovirus and the immunochromatographydetecting detecting test strip of occulting blood, perhaps comprise rotavirus and adenovirus immunochromatographydetecting detecting test strip and the immunochromatographydetecting detecting test strip of occulting blood, perhaps comprise rotavirus and occult blood immunochromatographydetecting detecting test strip and adenovirus immunochromatographydetecting detecting test strip.See also shown in Figure 1, in a specific embodiment of the present utility model, described rotavirus, adenovirus and the joint inspection kit of occulting blood comprise rotavirus, adenovirus and the immunochromatographydetecting detecting test strip of occulting blood, promptly include only a test strip, can detect rotavirus, adenovirus simultaneously and occult blood.See also Fig. 2 and shown in Figure 4, in two specific embodiments of the present utility model, described rotavirus, adenovirus and the joint inspection kit of occulting blood comprise rotavirus and the adenovirus immunochromatographydetecting detecting test strip and the immunochromatographydetecting detecting test strip of occulting blood, promptly comprise two test strip, detect rotavirus, adenovirus respectively simultaneously and occult blood.See also shown in Figure 3, in another specific embodiment of the present utility model, described rotavirus, adenovirus and the joint inspection kit of occulting blood comprise rotavirus immunochromatographydetecting detecting test strip, adenovirus immunochromatographydetecting detecting test strip and the immunochromatographydetecting detecting test strip of occulting blood, promptly comprise three test strip, detect rotavirus, adenovirus respectively simultaneously and occult blood.
Total institute is known, and immunochromatographydetecting detecting test strip generally is made up of sample pad, pad, chromatographic film and adsorptive pads from top to bottom successively; Be coated with immune labeled antibody on the described pad, be coated with antibody on the chromatographic film.During detection, thereby immune labeled antibody and antibody will combine formation sandwich structure respectively with detected material.
Preferably, have the immune labeled antibody of rotavirus, the immune labeled antibody of adenovirus and the immune labeled antibody of occulting blood on the pad of described rotavirus, adenovirus and the immunochromatographydetecting detecting test strip of occulting blood, be coated with rotavirus on the chromatographic film of described rotavirus, adenovirus and the immunochromatographydetecting detecting test strip of occulting blood and detect antibody, adenovirus detection antibody and the detection antibody of occulting blood.
Preferably, has the immune labeled antibody of rotavirus on the pad of described rotavirus immunochromatographydetecting detecting test strip, be coated with rotavirus on the chromatographic film of described rotavirus immunochromatographydetecting detecting test strip and detect antibody, have the immune labeled antibody of adenovirus on the pad of described adenovirus immunochromatographydetecting detecting test strip, be coated with adenovirus on the chromatographic film of described adenovirus immunochromatographydetecting detecting test strip and detect antibody.
Preferably, has the immune labeled antibody of rotavirus on the pad of described rotavirus immunochromatographydetecting detecting test strip, be coated with rotavirus on the chromatographic film of described rotavirus immunochromatographydetecting detecting test strip and detect antibody, have the immune labeled antibody of adenovirus on the pad of the described adenovirus and the immunochromatographydetecting detecting test strip of occulting blood and the immune labeled antibody of occulting blood, be coated with adenovirus on the chromatographic film of the described adenovirus and the immunochromatographydetecting detecting test strip of occulting blood and detect the antibody and the detection antibody of occulting blood.
Preferably, has immune labeled antibody of rotavirus and the immune labeled antibody of adenovirus on the pad of described rotavirus and adenovirus immunochromatographydetecting detecting test strip, be coated with rotavirus on the chromatographic film of described rotavirus and adenovirus immunochromatographydetecting detecting test strip and detect antibody and adenovirus detection antibody, have the immune labeled antibody of occulting blood on the pad of the described immunochromatographydetecting detecting test strip of occulting blood, be coated with the detection antibody of occulting blood on the chromatographic film of the described immunochromatographydetecting detecting test strip of occulting blood.
Preferably, has the immune labeled antibody of rotavirus on the pad of the described rotavirus and the immunochromatographydetecting detecting test strip of occulting blood and the immune labeled antibody of occulting blood, be coated with rotavirus on the chromatographic film of the described rotavirus and the immunochromatographydetecting detecting test strip of occulting blood and detect the antibody and the detection antibody of occulting blood, have the immune labeled antibody of adenovirus on the pad of described adenovirus immunochromatographydetecting detecting test strip, be coated with adenovirus on the chromatographic film of described adenovirus immunochromatographydetecting detecting test strip and detect antibody.More preferably, the immune labeled antibody of described rotavirus is the immune labeled antibody of anti-HRV, it is anti-HRV's antibody that described rotavirus detects antibody, the immune labeled antibody of described adenovirus is the immune labeled antibody of anti-adenovirus hominis, it is anti-adenovirus hominis antibody that described adenovirus detects antibody, the described immune labeled antibody of occulting blood is the immune labeled antibody of antihuman hemoglobin or the immune labeled antibody of anti-human transferrin or immune labeled antibody of anti-human erythrocyte matrix or the immune labeled antibody of anti-human albumin, and the described detection antibody of occulting blood is the immune labeled antibody of antihuman hemoglobin or the immune labeled antibody of anti-human transferrin or immune labeled antibody of anti-human erythrocyte matrix or the immune labeled antibody of anti-human albumin.
See also Fig. 1-shown in Figure 4, in four specific embodiments of the present utility model, the immune labeled antibody of described rotavirus is the immune labeled antibody of anti-HRV, and it is anti-HRV's antibody that described rotavirus detects antibody.Obviously, the immune labeled antibody of anti-HRV wherein and anti-HRV's antibodies HRV's different parts.
Immune labeled antibody adopts conventional immune labeled means mark.More preferably, the immune labeled antibody of described rotavirus is rotavirus colloid gold label antibody or rotavirus latex labelled antibody, the immune labeled antibody of described adenovirus is adenovirus colloid gold label antibody or adenovirus latex labelled antibody, and the described immune labeled antibody of occulting blood is occult blood the colloid gold label antibody or the latex labelled antibody of occulting blood.
See also Fig. 1-shown in Figure 4, in four specific embodiments of the present utility model, the immune labeled antibody of described rotavirus is rotavirus colloid gold label antibody, the immune labeled antibody of described adenovirus is adenovirus colloid gold label antibody, and the described immune labeled antibody of occulting blood is the colloid gold label antibody of occulting blood.
Described rotavirus and adenovirus immunochromatographydetecting detecting test strip and the described immunochromatographydetecting detecting test strip of occulting blood can adopt any suitable form to place.See also shown in Figure 4, in a specific embodiment more of the present utility model, described rotavirus, adenovirus and the joint inspection kit of occulting blood also comprise base plate, and described rotavirus and adenovirus immunochromatographydetecting detecting test strip and the described immunochromatographydetecting detecting test strip of occulting blood are fitted in respectively on the upper and lower surface of described base plate.See also shown in Figure 2ly, in another specific embodiment of the present utility model, described rotavirus and adenovirus immunochromatographydetecting detecting test strip and the described immunochromatographydetecting detecting test strip of occulting blood are set up in parallel, and specifically are arranged on the base plate.
Described rotavirus immunochromatographydetecting detecting test strip, described adenovirus immunochromatographydetecting detecting test strip and the described immunochromatographydetecting detecting test strip of occulting blood can adopt any suitable form to place.See also shown in Figure 3ly, in another specific embodiment of the present utility model, described rotavirus immunochromatographydetecting detecting test strip, described adenovirus immunochromatographydetecting detecting test strip and the described immunochromatographydetecting detecting test strip of occulting blood are set up in parallel.Certainly, the specific embodiment that it also can be as shown in Figure 4 is such, is fitted in respectively on the upper and lower surface of described base plate.
Rotavirus of the present utility model, adenovirus and the joint inspection kit of occulting blood can also comprise other common reagent of immunochromatographytest test kit, for example sample sustained-release liquid or the like.
The preparation process of the test strip of rotavirus of the present utility model, adenovirus and the joint inspection kit of occulting blood is summarized as follows, should be understood that these embodiment only are used to the utility model is described and are not used in restriction scope of the present utility model.The experimental technique of unreceipted actual conditions in the following example, usually according to normal condition, people such as Sambrook for example, molecular cloning: laboratory manual (New York:Cold Spring Harbor Laboratory Press, 1989) condition described in, or the condition of advising according to manufacturer.
Antibody (anti-HRV's antibody) the colloid gold label method that is used for the rotavirus detection is as follows: regulate 50ml colloidal gold solution pH value to 7.7 with solution of potassium carbonate; Under stirring condition, dropwise slowly 0.65ml1mg/ml anti-rotavirus antibody to be marked is added in the above-mentioned colloidal gold solution, mixing, room temperature leaves standstill 30min; The 12.5ml confining liquid is slowly added in the above-mentioned colloidal gold solution, mixing, room temperature leaves standstill 30min; 8000g4 ℃ of centrifugal 1h removes supernatant, stays loose deposits; Each pipe precipitation is dissolved with the 25ml cleansing solution, and 6000g4 ℃ of centrifugal 1h removes supernatant, stays loose deposits; Each pipe precipitation changes in the 1.5ml centrifuge tube with the dissolving of 1.2ml cleansing solution, and 4500g4 ℃ of centrifugal 1h removes supernatant; All precipitations are resuspended with the resuspended liquid of 1ml colloidal gold antibody, 4 ℃ of preservations.
Be used for the antibody (anti-adenovirus hominis antibody) of adenovirus diagnosis and antibody (antihuman hemoglobin antibody or anti-human transferrin antibody or anti-human erythrocyte matrix antibody or anti-human albumin antibody) the colloid gold label method diagnosed of being used to occult blood the same.
The specking of embodiment 2 collaurum pads
The specking method of antibody (anti-HRV's antibody) collaurum pad that is used for rotavirus diagnosis is as follows: the colloidal gold antibody solution dilution: with the resuspended liquid of colloidal gold antibody with 4 times of the colloidal gold antibody bond dilutions of above-mentioned preparation; Set point film instrument, the power supply of opening point film instrument is set the specking program, and the specking amount is 8ul/cm; No. 1 pipeline is the specking passage; Point film instrument initialization: No. 1 pipeline is placed the resuspended solution of colloidal gold antibody, select initialize routine, 6 circulations of initialization; Specking: gold mark pad is lain on the film instrument by the fixed position, begin specking by " GO " key on the control panel, take off after having put, check the gold mark pad that specking is good, the colloidal gold antibody band of specking evenly, continuously and the straight line that connects whole gold mark pad be qualified specking product, occurring breakpoint in two straight lines is defective specking product; Whenever putting a slice gold mark pad, is specking once (a slice) by " GO " key on control panel; Specking finishes, and the gold of specking is marked pad placed the room temperature air dry 1 hour, should can't see the specking vestige on the film.
Be used for the antibody (anti-adenovirus hominis antibody) of adenovirus diagnosis and antibody (antihuman hemoglobin antibody or anti-human transferrin antibody or anti-human erythrocyte matrix antibody or anti-human albumin antibody) the collaurum pad specking method diagnosed of being used to occult blood the same.
The preparation of embodiment 3 chromatographic film
Antibody (anti-HRV's antibody) the chromatographic film preparation method who is used for the rotavirus diagnosis is as follows: get mouse-anti HRV antibody 500ug, be added in the 5ml graduated centrifuge tube, antibody diluent is to 1ml, and Container Tag T indicates.Get sheep anti-mouse igg antibody 25ul, be added in the 5ml graduated centrifuge tube, antibody diluent is to 1ml, and Container Tag C indicates.Set point film instrument, the power supply of opening point film instrument is set the specking program, and the specking amount is 1ul/cm; No. 1 pipeline is for detecting band specking passage, and No. 2 pipeline is contrast band specking passage; Point film instrument initialization: No. 1 pipeline is placed detection band solution, No. 2 pipelines are placed contrast band solution, select initialize routine, 6 circulations of initialization; Specking: chromatographic film is lain on the film instrument by the fixed position, begin specking by " GO " key on the control panel, take off after having put, check the good chromatographic film of specking, detect band and contrast band and be two evenly, continuously and the straight line that connects whole chromatographic film be qualified specking product, occurring breakpoint in two straight lines is defective specking product; Whenever putting a slice chromatographic film, is specking once (a slice) by " GO " key on control panel; Specking finishes, and the chromatographic film of specking was placed the room temperature air dry 1 hour, should can't see the specking vestige on the film.
Be used for the antibody (anti-adenovirus hominis antibody) of adenovirus diagnosis and antibody (antihuman hemoglobin antibody or anti-human transferrin antibody or anti-human erythrocyte matrix antibody or anti-human albumin antibody) the chromatographic film preparation method that diagnoses of being used to occult blood the same.
The protection sheet of wider portion on the base plate is removed,,, be attached on the base plate plate in C line mode up with drawing the chromatographic film of good line along the lower limb of top protection sheet; The collaurum pad is attached to T line below, and a little contacts with the NC film; Sample pad is attached to collaurum pad below, and a little contacts with the collaurum pad; Then remove the top protection sheet, adsorptive pads is attached to the top of NC film, a little contacts with the NC film; Protection sheet and index strip paper are attached to the test strips outside that assembles one by one, are assembled into kilocalorie.
Connect the cutting machine power supply, set and cut the film program, the setting cutting width is 4mm; The kilocalorie certified products are kept flat in the cutting machine platform track, face up, press " GO " key on the guidance panel, begin cutting; Whenever put a slice kilocalorie certified products, press on the guidance panel " GO " key once, until having cut all kilocalorie certified products; After cutting is finished, will be used to detect rotavirus, adenovirus respectively and the test strips of occulting blood sticks in base plate side by side, form rotavirus, adenovirus and the joint inspection test strips of occulting blood of block form.
The assembling of embodiment 5 kits
Get aluminium foil bag and drying agent; Open heat sealing machine, preheating; Test strips to be packed, 1 bag of drying agent are packed in the aluminium foil bag; Length is according to the rules cut off the aluminium foil bag that test strips and drying agent are housed; Seal aluminium foil bag with heat sealing machine; Labelled.
The checking of embodiment 6 clinical samples
With above-mentioned test strips, verify with clinical sample.
Test strips test side (left end of test strips among Fig. 1-Fig. 4) has adsorptive pads, inserts in the sample during reaction.
If band nature controlling line 6 appears in the C district during detection, then the T district band occurs and represents that promptly sample is positive; Band do not occur and then represent feminine gender.
If band does not appear in the C district during detection, represent that then test strips lost efficacy.
Testing result is as follows:
The detection sensitivity of this test strips is: (16+29+29)/(16+29+29) * and 100%=100%;
The detection specificity of this test strips is: (21+67+82+138)/(21+67+82+138) * and 100%=100%.
The foregoing description result shows that the accuracy rate of this collaurum detection is very high, satisfies clinical practice.
Detect rotavirus, adenovirus simultaneously and three indexs of occulting blood very necessary, by use of the present utility model, to the children's diarrhae due to rotavirus and the adenovirus, because of symptom lighter, the course of disease is shorter, and is less to children's harm, gets final product so only carry out general treatment; The positive of occulting blood then illustrates it is hemorrhagic enteritis, and the course of disease is long, and harm is big, need carry out in time and effectively treatment.
To sum up, rotavirus of the present utility model, adenovirus and the joint inspection kit of occulting blood can be diagnosed rotavirus, adenovirus simultaneously and occult blood by primary sample, use easy, the joint inspection result for next step accurately treatment strong foundation is provided, be suitable for large-scale promotion application.
In this instructions, the utility model is described with reference to its certain embodiments.But, still can make various modifications and conversion obviously and not deviate from spirit and scope of the present utility model.Therefore, instructions and accompanying drawing are regarded in an illustrative, rather than a restrictive.
Claims (5)
1. a rotavirus, adenovirus and the joint inspection kit of occulting blood, it is characterized in that, comprise rotavirus, adenovirus and the immunochromatographydetecting detecting test strip of occulting blood, have the immune labeled antibody of rotavirus, the immune labeled antibody of adenovirus and the immune labeled antibody of occulting blood on the pad of described rotavirus, adenovirus and the immunochromatographydetecting detecting test strip of occulting blood, be coated with rotavirus on the chromatographic film of described rotavirus, adenovirus and the immunochromatographydetecting detecting test strip of occulting blood and detect antibody, adenovirus detection antibody and the detection antibody of occulting blood;
Perhaps comprise the rotavirus immunochromatographydetecting detecting test strip, the adenovirus immunochromatographydetecting detecting test strip and the immunochromatographydetecting detecting test strip of occulting blood, has the immune labeled antibody of rotavirus on the pad of described rotavirus immunochromatographydetecting detecting test strip, be coated with rotavirus on the chromatographic film of described rotavirus immunochromatographydetecting detecting test strip and detect antibody, has the immune labeled antibody of adenovirus on the pad of described adenovirus immunochromatographydetecting detecting test strip, be coated with adenovirus on the chromatographic film of described adenovirus immunochromatographydetecting detecting test strip and detect antibody, have the immune labeled antibody of occulting blood on the pad of the described immunochromatographydetecting detecting test strip of occulting blood, be coated with the detection antibody of occulting blood on the chromatographic film of the described immunochromatographydetecting detecting test strip of occulting blood;
Perhaps comprise rotavirus immunochromatographydetecting detecting test strip and adenovirus and the immunochromatographydetecting detecting test strip of occulting blood, has the immune labeled antibody of rotavirus on the pad of described rotavirus immunochromatographydetecting detecting test strip, be coated with rotavirus on the chromatographic film of described rotavirus immunochromatographydetecting detecting test strip and detect antibody, have the immune labeled antibody of adenovirus on the pad of the described adenovirus and the immunochromatographydetecting detecting test strip of occulting blood and the immune labeled antibody of occulting blood, be coated with adenovirus on the chromatographic film of the described adenovirus and the immunochromatographydetecting detecting test strip of occulting blood and detect the antibody and the detection antibody of occulting blood;
Perhaps comprise rotavirus and adenovirus immunochromatographydetecting detecting test strip and the immunochromatographydetecting detecting test strip of occulting blood, has immune labeled antibody of rotavirus and the immune labeled antibody of adenovirus on the pad of described rotavirus and adenovirus immunochromatographydetecting detecting test strip, be coated with rotavirus on the chromatographic film of described rotavirus and adenovirus immunochromatographydetecting detecting test strip and detect antibody and adenovirus detection antibody, have the immune labeled antibody of occulting blood on the pad of the described immunochromatographydetecting detecting test strip of occulting blood, be coated with the detection antibody of occulting blood on the chromatographic film of the described immunochromatographydetecting detecting test strip of occulting blood;
Perhaps comprise rotavirus and occult blood immunochromatographydetecting detecting test strip and adenovirus immunochromatographydetecting detecting test strip, has the immune labeled antibody of rotavirus on the pad of the described rotavirus and the immunochromatographydetecting detecting test strip of occulting blood and the immune labeled antibody of occulting blood, be coated with rotavirus on the chromatographic film of the described rotavirus and the immunochromatographydetecting detecting test strip of occulting blood and detect the antibody and the detection antibody of occulting blood, have the immune labeled antibody of adenovirus on the pad of described adenovirus immunochromatographydetecting detecting test strip, be coated with adenovirus on the chromatographic film of described adenovirus immunochromatographydetecting detecting test strip and detect antibody.
2. rotavirus according to claim 1, the adenovirus and the joint inspection kit of occulting blood, it is characterized in that, the immune labeled antibody of described rotavirus is the immune labeled antibody of anti-HRV, it is anti-HRV's antibody that described rotavirus detects antibody, the immune labeled antibody of described adenovirus is the immune labeled antibody of anti-adenovirus hominis, it is anti-adenovirus hominis antibody that described adenovirus detects antibody, the described immune labeled antibody of occulting blood is the immune labeled antibody of antihuman hemoglobin or the immune labeled antibody of anti-human transferrin or immune labeled antibody of anti-human erythrocyte matrix or the immune labeled antibody of anti-human albumin, and the described detection antibody of occulting blood is the immune labeled antibody of antihuman hemoglobin or the immune labeled antibody of anti-human transferrin or immune labeled antibody of anti-human erythrocyte matrix or the immune labeled antibody of anti-human albumin.
3. rotavirus according to claim 1, adenovirus and the joint inspection kit of occulting blood, it is characterized in that, the immune labeled antibody of described rotavirus is rotavirus colloid gold label antibody or rotavirus latex labelled antibody, the immune labeled antibody of described adenovirus is adenovirus colloid gold label antibody or adenovirus latex labelled antibody, and the described immune labeled antibody of occulting blood is occult blood the colloid gold label antibody or the latex labelled antibody of occulting blood.
4. rotavirus according to claim 1, adenovirus and the joint inspection kit of occulting blood, it is characterized in that described rotavirus immunochromatographydetecting detecting test strip, described adenovirus immunochromatographydetecting detecting test strip and the described immunochromatographydetecting detecting test strip of occulting blood are set up in parallel.
5. rotavirus according to claim 1, adenovirus and the joint inspection kit of occulting blood, it is characterized in that, described rotavirus, adenovirus and the joint inspection kit of occulting blood also comprise base plate, and described rotavirus immunochromatographydetecting detecting test strip, described adenovirus immunochromatographydetecting detecting test strip and the described immunochromatographydetecting detecting test strip of occulting blood are fitted in respectively on the upper and lower surface of described base plate.
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| CN 201320052873 CN203101395U (en) | 2013-01-30 | 2013-01-30 | Rotavirus, adenovirus and occult blood joint inspection kit |
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| CN 201320052873 CN203101395U (en) | 2013-01-30 | 2013-01-30 | Rotavirus, adenovirus and occult blood joint inspection kit |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103543273A (en) * | 2013-11-04 | 2014-01-29 | 无锡博慧斯生物医药科技有限公司 | Joint inspection kit for pregnant woman premature birth fetus fibronectin and phosphorylation insulin-like growth factor binding protein-1 |
| CN103969434A (en) * | 2013-01-30 | 2014-08-06 | 无锡博慧斯生物医药科技有限公司 | Rotavirus-adenovirus-occult blood combined-measuring kit |
| EP4427842A1 (en) * | 2023-03-10 | 2024-09-11 | Lockcon AG | Sampling device, sampling system and method for operating a sampling device |
| DE102023112897A1 (en) | 2023-03-10 | 2024-09-12 | Lockcon Ag | Sampling device, sampling system and method for operating a sampling device |
-
2013
- 2013-01-30 CN CN 201320052873 patent/CN203101395U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103969434A (en) * | 2013-01-30 | 2014-08-06 | 无锡博慧斯生物医药科技有限公司 | Rotavirus-adenovirus-occult blood combined-measuring kit |
| CN103543273A (en) * | 2013-11-04 | 2014-01-29 | 无锡博慧斯生物医药科技有限公司 | Joint inspection kit for pregnant woman premature birth fetus fibronectin and phosphorylation insulin-like growth factor binding protein-1 |
| EP4427842A1 (en) * | 2023-03-10 | 2024-09-11 | Lockcon AG | Sampling device, sampling system and method for operating a sampling device |
| DE102023112897A1 (en) | 2023-03-10 | 2024-09-12 | Lockcon Ag | Sampling device, sampling system and method for operating a sampling device |
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