CN203616314U - LH (luteinizing hormone) and BV (bacterial vaginitis) combined detection kit - Google Patents
LH (luteinizing hormone) and BV (bacterial vaginitis) combined detection kit Download PDFInfo
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- CN203616314U CN203616314U CN201320690827.0U CN201320690827U CN203616314U CN 203616314 U CN203616314 U CN 203616314U CN 201320690827 U CN201320690827 U CN 201320690827U CN 203616314 U CN203616314 U CN 203616314U
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Abstract
The utility model relates to an LH (luteinizing hormone) and BV (bacterial vaginitis) combined detection kit, and belongs to the technical field of detection. The LH and BV combined detection kit comprises one or more of LH immunochromatography detection test paper, pH test paper, sialidase detection test paper, leukocyte esterase detection test paper, hydrogen peroxide detection test paper and amine test paper; and preferably, a combination pad and a chromatography film of the immunochromatography test paper are coated with corresponding immune labelled antibodies and detection antibodies respectively, and one or more of the LH immunochromatography detection test paper, the pH test paper, the sialidase detection test paper, the leukocyte esterase detection test paper, the hydrogen peroxide detection test paper and the amine test paper are parallelly arranged or put on the upper surface and the lower surface of a baseboard. The detection kit can simultaneously detect LH and BV through self-sampling, the combined detection result provides strong evidence for prenatal and postnatal care and reasonable contraception, and the detection kit is simple, convenient and suitable for large-scale popularization and application.
Description
Technical field
The utility model relates to a kind of interstitialcellstimulating hormone (ICSH) and bacterial vaginitis joint inspection kit, and particularly a kind of interstitialcellstimulating hormone (ICSH) (LH) and bacterial vaginitis (BV) joint inspection kit, belong to detection technique field.
Background technology
Interstitialcellstimulating hormone (ICSH) (LH) is secreted by adenohypophysis basophil, is present in people's blood and urine, and its effect is the release that stimulates mature egg in ovary.LH concentration in urine significantly rises suddenly about 36-48 hour conventionally before ovulation, peaks at 14-28 hour, and behind peak, approximately 14 ~ 28 hours membrana follicularises break, and discharges mature egg.In the 1-3 of women behind LH peak days, the most easily become pregnant, thereby, detect the exist level of LH in urine and can be used in the predicting ovulation time, thus rationally contraception or conceived.
Bacterial vaginitis (BV) refers to that a class shows as genital tract normal flora and (produces H on bacteriology
2o
2lactobacillus) quantity reduces, and replaces the clinical symptom grouping of one group of anaerobism group (Bacteroides family, Gardnerella, Mo Bilun Bordetella, mycoplasma hominis belong to and Peptostreptococcus etc.) quantity due to increasing.BV can lead to grave consequences to mother and fetus and baby's health, as: uterine cavity infects, and pelvic infection and fine hair amnion infect, PNI, and threatened abortion, premature labor, premature rupture of fetal membranes, the infant of low-birth weight etc., thereby its Accurate Diagnosis is had very important significance.Clinical standard is Amsel goldstandard: 1. vaginal fluid is emulsion form; 2. pH>4.5; 3. the amine test positive; 4. in vaginal fluid, detect clues cell (>20%).If 4. negative, but the visible a large amount of gram-negative coccobacilluses of smear Gram’s staining, nothing or accidental lactic acid bacteria also can be judged to the BV positive.Said method all has experience requirement to reviewer, and diagnostic criteria is difficult for grasping, and needs microscope smear, wastes time and energy, and is not easy to the clinical fast detecting of outpatient service.
Summary of the invention
The purpose of this utility model is to overcome above-mentioned weak point, a kind of interstitialcellstimulating hormone (ICSH) (LH) and bacterial vaginitis (BV) joint inspection kit are provided, sample by oneself, can fast detecting bacterial vaginitis, simultaneously can the Precise detection of ovulation time, increase and become pregnant probability or reach reasonable contraception.
The technical scheme providing according to the utility model, a kind of interstitialcellstimulating hormone (ICSH) and bacterial vaginitis joint inspection kit, comprise the first base plate, in the one side of the first base plate, is provided with chromatographic film, the front end of chromatographic film arranges opaque diaphragm, and the front end of opaque diaphragm arranges arrow; The front end of arrow arranges pad; In chromatographic film, be also disposed with detection line and nature controlling line, chromatographic film rear end is provided with adsorptive pads.
Also comprise infection index detection zone.
Described infection index detection zone is attached on the second base plate or is compound on any one surface of the first base plate.
In the one side of the first base plate, be provided with chromatographic film, the front end of chromatographic film arranges pad, and the front of pad also arranges sample pad; In chromatographic film, be also disposed with detection line and nature controlling line, chromatographic film rear end is provided with adsorptive pads; On the lower surface of the first base plate, be also provided with infection index detection zone.
Described infection index detection zone is provided with pH test paper, sialidase Test paper, leukocyte esterase Test paper, one or more in hydrogen peroxide Test paper and amine test paper.
The described pH test paper that adheres to, sialidase Test paper, leukocyte esterase Test paper, the first base plate of hydrogen peroxide Test paper and amine test paper or the material of the second base plate are the one in PET, PVC, PE, PP or PS, long is 10-300mm, wide 2-30mm, and thickness is 0.1-1.5mm.
Described pH test paper, sialidase Test paper, leukocyte esterase Test paper, the basic material of hydrogen peroxide Test paper and amine test paper is cellulose filter paper, glass fiber filter paper, chromatographic paper, polyester film or nylon membrane; Described test paper be shaped as square, rectangle, circle, ellipse, triangle, rhombus or trapezoidal; Size is long 1-30 mm; Wide 1-30 mm; Thickness 0.01-5 mm; Spacing between multiple test paper is 1-30 mm.
On described pad, there is the immune labeled antibody of interstitialcellstimulating hormone (ICSH) LH.
In described chromatographic film, be coated with interstitialcellstimulating hormone (ICSH) LH and detect antibody.
The beneficial effects of the utility model: the utility model can sample by oneself, can fast detecting bacterial vaginitis, can accurately differentiate whether there is early pregnancy simultaneously, simple and convenient, is applicable to large-scale promotion application.
Accompanying drawing explanation
Fig. 1 is embodiment 1 first base plate 10 structural representations.
Fig. 2 is embodiment 1 second base plate 11 structural representations.
Fig. 3 is embodiment 2 structural representations.
Fig. 4 is embodiment 3 structural representations.
Fig. 5 is embodiment 4 structural representations.
Fig. 6 is embodiment 1 infection index detection zone structural representation.
Fig. 7 is embodiment 2 infection index detection zone structural representations.
Fig. 8 is embodiment 3 infection index detection zone structural representations.
Fig. 9 is embodiment 4 infection index detection zone structural representations.
Embodiment
As shown in Fig. 1,2,5, a kind of interstitialcellstimulating hormone (ICSH) and bacterial vaginitis joint inspection kit, comprise the first base plate 10, is provided with chromatographic film 6 in the one side of the first base plate 10, the front end of chromatographic film 6 arranges opaque diaphragm 3, and the front end of opaque diaphragm 3 arranges arrow 2; The front end of arrow 2 arranges pad 1; In chromatographic film 6, be also disposed with detection line 4 and nature controlling line 5, chromatographic film 6 rear ends are provided with adsorptive pads 7.
Also comprise infection index detection zone 8.Described infection index detection zone 8 is attached to the upper surface of the first base plate 10.
Described infection index detection zone 8 is provided with described pH test paper.Comprise LH immunochromatographydetecting detecting test strip, and urinary system infection contamination index detection zone, comprise described pH test paper, be placed in the upper surface of base plate 10 with horizontal coverage mode or built-in embedded mode.
As shown in Fig. 3,7, a kind of interstitialcellstimulating hormone (ICSH) and bacterial vaginitis joint inspection kit, comprise the first base plate 10, is provided with chromatographic film 6 in the one side of the first base plate 10, the front end of chromatographic film 6 arranges opaque diaphragm 3, and the front end of opaque diaphragm 3 arranges arrow 2; The front end of arrow 2 arranges pad 1; In chromatographic film 6, be also disposed with detection line 4 and nature controlling line 5, chromatographic film 6 rear ends are provided with adsorptive pads 7.
Also comprise infection index detection zone 8.Described infection index detection zone 8 is attached on the second base plate 11.
Described infection index detection zone 8 is provided with sialidase Test paper, leukocyte esterase Test paper.Be placed in the upper surface of base plate with horizontal coverage mode or built-in embedded mode, and be set up in parallel on draw-in groove with LH immunochromatographydetecting detecting test strip.
As shown in Fig. 4,8, a kind of interstitialcellstimulating hormone (ICSH) and bacterial vaginitis joint inspection kit, comprise the first base plate 10, is provided with chromatographic film 6 in the one side of the first base plate 10, and the front end of chromatographic film 6 arranges pad 1, and the front of pad 1 also arranges sample pad 9; In chromatographic film 6, be also disposed with detection line 4 and nature controlling line 5, chromatographic film 6 rear ends are provided with adsorptive pads 7; On the lower surface of the first base plate 10, be also provided with infection index detection zone 8.
Described infection index detection zone 8 is attached on the lower surface of the first base plate 10.
Described infection index detection zone 8 is provided with sialidase Test paper, leukocyte esterase Test paper, hydrogen peroxide Test paper, is placed in the surface of base plate with horizontal coverage mode or built-in embedded mode, and is placed in respectively the upper and lower surface of base plate with LH immunochromatographydetecting detecting test strip.
As shown in Fig. 5,9, a kind of interstitialcellstimulating hormone (ICSH) and bacterial vaginitis joint inspection kit, comprise the first base plate 10, is provided with chromatographic film 6 in the one side of the first base plate 10, the front end of chromatographic film 6 arranges opaque diaphragm 3, and the front end of opaque diaphragm 3 arranges arrow 2; The front end of arrow 2 arranges pad 1; In chromatographic film 6, be also disposed with detection line 4 and nature controlling line 5, chromatographic film 6 rear ends are provided with adsorptive pads 7.Described infection index detection zone 8 is attached on the second base plate 11.
In embodiment 1-4, the immune labeled antibody of described LH is anti-human LH colloid gold immune labelled antibody, and it is that anti-human LH detects antibody that described LH detects antibody, and anti-human LH colloid gold immune labelled antibody wherein and anti-human LH detect the different parts of antibody in conjunction with people LH.
Described infection index detection zone 8 is provided with sialidase Test paper, leukocyte esterase Test paper, hydrogen peroxide Test paper and amine test paper, be placed in the upper surface of base plate, and be placed on dismountable draw-in groove with LH immunochromatographydetecting detecting test strip with horizontal coverage mode or built-in embedded mode.
Described pH test paper is dry processing after blank filter paper is soaked in the solution of methyl orange and bromcresol green composition, and the concentration of described methyl orange in solution is 10 ~ 600 mg/L, and the concentration of described bromcresol green in solution is 10 ~ 300 mg/L;
Described sialidase Test paper is for to be immersed in the chloro-3-indoles of the bromo-4-of 5-acetyl neuraminic acid salt by blank filter paper, NBT, dry processing after soaking in the solution of trehalose and PBS damping fluid composition, the concentration of the chloro-3-indoles of the bromo-4-of described 5-acetyl neuraminic acid salt in solution is 5 ~ 200 mg/L, the concentration of described NBT in solution is 10 ~ 500 mg/L, described trehalose is 200 ~ 10000 mg/L in the concentration in solution, the pH value of described PBS is 6.5, and volumetric molar concentration is 0.01 ~ 1 M;
Described leukocyte esterase Test paper is dry processing after blank filter paper is soaked in the solution of the chloro-3-indolyl acetic acid salt of the bromo-4-of 5-and sucrose composition, the concentration of the chloro-3-indolyl acetic acid salt of the bromo-4-of described 5-in solution is 40 ~ 1000 mg/L, and the concentration of described sucrose in solution is 200 ~ 10000 mg/L;
Described hydrogen peroxide Test paper be by blank filter paper at peroxidase, dry processing after soaking in the enzyme solutions of surfactant and tetramethyl benzidine (TMB) composition, the concentration of described peroxidase in solution is 1.0-3.0 × 10
5u/L, the concentration of surfactant in solution is 0.1-10 %, the concentration of TMB in enzyme solutions is 200-1000 mg/L;
Described amine test paper is that after blank filter paper is soaked in potassium hydroxide solution, the dry reagent piece A processing is pasted on the rear dry reagent piece B processing of immersion in bromcresol green and Triton X-100 solution, wherein in A reagent piece, concentration of potassium hydroxide is 400-10000 mg/L, in B reagent piece, bromcresol green concentration is that 100 ~ 1000, Triton X-100 is 0.1 ~ 10 %.
Sample dilution is 0.9 % NaCl.
Application Example 1
Use the operation steps of the utility model interstitialcellstimulating hormone (ICSH) (LH) and bacterial vaginitis (BV) joint inspection kit as follows:
(1) getting secretion with cotton swab from posterior fornix is first directly coated in pH test paper;
(2) get secretion with cotton swab from posterior fornix again, add the sample diluted secretion of 300 ~ 500 μ L;
(3) respectively at LH immunochromatographydetecting detecting test strip, sialidase Test paper, leukocyte esterase Test paper, drips the secretion of appropriate dilution on hydrogen peroxide Test paper and amine test paper.
(4) test strips is at room temperature left standstill about 10 minutes or leaves standstill visual colorimetric determination after about 10 minutes in 37 ℃ of constant temperature ovens.
Sentence read result: if band appears in LH immunochromatographydetecting detecting test strip C district when detection, T district occurs that band represents that sample is positive, does not occur that band represents feminine gender; If band does not appear in C district, represent that test strips lost efficacy.PH test paper occurs red or light green expression is normal, occurs that bottle green represents undesired.Sialidase Test paper does not develop the color and represents normally, to occur that purple or brown represent undesired.Leukocyte esterase Test paper does not develop the color and represents normally, occurs that blue or green represents undesired.There is blue expression normally in hydrogen peroxide Test paper, not developing the color, it is undesired to represent.Amine test paper does not develop the color and represents normally, occurs that green expression is undesired.
Preparation Example 1
The antibody colloidal gold labeling method detecting for LH is for adding 200 μ L, and 3% sal tartari regulates the pH of 50 mL colloidal gold solutions; Under stirring condition, add gradually 0.15 mL, 6.6 mg/mL anti-LH antibody to be marked, in above-mentioned colloidal gold solution, mixes, and room temperature leaves standstill 30 min; 12.5 mL confining liquids are slowly added in above-mentioned colloidal gold solution, mix, room temperature leaves standstill 30 min; The centrifugal 1h of 8000 4 ℃ of g, removes supernatant, stays loose deposits; Each pipe precipitation is dissolved with 25 mL cleansing solutions, and the centrifugal 1h of 6,000 4 ℃ of g, removes supernatant, stays loose deposits; Each pipe precipitation is dissolved with 1.2 mL cleansing solutions, proceeds in 1.5 mL centrifuge tubes, and the centrifugal 1h of 4,500 4 ℃ of g, removes supernatant; All precipitations are resuspended with the resuspended liquid of 1 mL colloidal gold antibody, 4 ℃ of preservations.
The specking method of the antibody colloidal gold pad detecting for LH is as follows: use the resuspended liquid of colloidal gold antibody by 4 times of the colloidal gold antibody bond dilutions of above-mentioned preparation; The power supply of opening point film instrument, sets specking program, and specking amount is 8 μ L/cm; No. 1 pipeline is specking passage; No. 1 pipeline is placed in to the resuspended solution of colloidal gold antibody, selects initialize routine, 6 circulations of initialization; Gold mark pad is lain on a film instrument by fixed position, press " GO " key on control panel and start specking, after having put, take off, check the good gold mark pad of specking, the colloidal gold antibody band of specking evenly, continuously and the straight line that connects whole gold mark pad be qualified specking product, in two straight lines, occur that breakpoint is defective specking product; Often put a slice gold mark pad, " GO " key of pressing on a control panel is once (a slice) of specking; Specking finishes, and the gold mark pad of specking is placed in to room temperature natural drying 1 hour, on film, should can't see specking vestige.
The antibody chromatography membrane preparation method detecting for LH is as follows: get mouse-anti people LH antibody 500 ug, be added in 5 mL graduated centrifuge tubes, antibody diluent to 1 mL, Container Tag T mark.Get sheep anti-mouse igg antibody 25 μ L, be added in 5 mL graduated centrifuge tubes, antibody diluent to 1 mL, Container Tag C mark; The power supply of opening point film instrument, sets specking program, and specking amount is 1 μ L/cm, and No. 1 pipeline is for detecting band specking passage, and No. 2 pipelines are contrast band specking passage; No. 1 pipeline is placed in and detects band solution, No. 2 pipelines are placed in to contrast band solution, select initialize routine, 6 circulations of initialization; Chromatographic film is lain on a film instrument by fixed position, press " GO " key on control panel and start specking, after having put, take off, check the good chromatographic film of specking, detect band and contrast band and be two evenly, continuously and the straight line that connects whole chromatographic film be qualified specking product, in two straight lines, occur that breakpoint is defective specking product; Often put a slice chromatographic film, " GO " key of pressing on a control panel is once (a slice) of specking; Specking finishes, and the chromatographic film of specking is placed in to room temperature natural drying 1 hour, on film, should can't see specking vestige.
The protection sheet of wider portion on base plate is removed, and the edge lower limb of protection sheet above, will pull the chromatographic film of line, is attached on base plate plate in C line mode up; Collaurum pad is attached to T line below, and a little contacts with NC film; Sample pad is attached to collaurum pad below, and a little contacts with collaurum pad; Then remove top protection sheet, adsorptive pads is attached to the top of NC film, a little contacts with NC film; Protection sheet and index strip paper are attached to the test strips outside assembling one by one, are assembled into kilocalorie.Connect cutting machine power supply, set and cut film program, setting cutting width is 4mm; Kilocalorie certified products are kept flat in cutting machine platform track, face up, press " GO " key on guidance panel, start cutting; Often put a slice kilocalorie certified products, press on guidance panel " GO " key once, until cut all kilocalorie certified products.
PH test paper, sialidase Test paper, leukocyte esterase Test paper, the filter paper material of hydrogen peroxide Test paper and amine test paper is chromatographic paper and nylon membrane, the material that base plate uses is transparent PVC sheet.
PH test paper is made: it is 300 mg/L that filter paper is immersed in to methyl orange concentration, and bromcresol green concentration is in the solution of 200 mg/L, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm;
Sialidase Test paper is made: it is 200 mg/L that filter paper is immersed in to the chloro-3-indoles of the bromo-4-of 5-acetyl neuraminic acid salinity, NBT concentration is 400 mg/L, trehalose concentration is 2000 mg/L, the pH value of PBS damping fluid is 6.5, volumetric molar concentration is in the solution of 0.01 M, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm;
Leukocyte esterase Test paper is made: it is 300 mg/L that filter paper is immersed in to the chloro-3-indolyl acetic acid salinity of the bromo-4-of 5-, and sucrose concentration is in the solution of 2000 mg/L, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm;
Hydrogen peroxide Test paper is made: it is 2.0 × 10 that filter paper is immersed in to peroxidase concn
5u/L, the concentration of surfactant in solution is 0.2 %, the concentration of TMB in enzyme solutions is in the solution of 700 mg/L, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm;
Amine test paper is made: reagent piece A is immersed in filter paper in the solution that concentration of potassium hydroxide is 10000 mg/L, dries; Reagent piece B is 500 mg/L for nylon membrane is immersed in to bromcresol green concentration, in the solution that Triton X-100 is 0.2%, dries.By reagent piece A and B with being cut into cutting machine the square that the length of side is 4 mm after adhered by double sided plaster;
Respectively by pH test paper, sialidase Test paper, leukocyte esterase Test paper, any one in hydrogen peroxide Test paper and amine test paper covers with level or the stickup of built-in embedded mode is fixed on transparent PVC sheet, each test paper spacing is 5 mm, is cut into the length of side and is the test strips of 4 mm.
Preparation Example 2
The antibody colloidal gold labeling method detecting for LH is for adding 200 μ L, and 3% sal tartari regulates the pH of 50 mL colloidal gold solutions; Under stirring condition, add gradually 0.15 mL, 6.6 mg/mL anti-LH antibody to be marked, in above-mentioned colloidal gold solution, mixes, and room temperature leaves standstill 30 min; 12.5 mL confining liquids are slowly added in above-mentioned colloidal gold solution, mix, room temperature leaves standstill 30 min; The centrifugal 1h of 8000 4 ℃ of g, removes supernatant, stays loose deposits; Each pipe precipitation is dissolved with 25 mL cleansing solutions, and the centrifugal 1h of 6,000 4 ℃ of g, removes supernatant, stays loose deposits; Each pipe precipitation is dissolved with 1.2 mL cleansing solutions, proceeds in 1.5 mL centrifuge tubes, and the centrifugal 1h of 4,500 4 ℃ of g, removes supernatant; All precipitations are resuspended with the resuspended liquid of 1 mL colloidal gold antibody, 4 ℃ of preservations.
The specking method of the antibody colloidal gold pad detecting for LH is as follows: use the resuspended liquid of colloidal gold antibody by 4 times of the colloidal gold antibody bond dilutions of above-mentioned preparation; The power supply of opening point film instrument, sets specking program, and specking amount is 8 μ L/cm; No. 1 pipeline is specking passage; No. 1 pipeline is placed in to the resuspended solution of colloidal gold antibody, selects initialize routine, 6 circulations of initialization; Gold mark pad is lain on a film instrument by fixed position, press " GO " key on control panel and start specking, after having put, take off, check the good gold mark pad of specking, the colloidal gold antibody band of specking evenly, continuously and the straight line that connects whole gold mark pad be qualified specking product, in two straight lines, occur that breakpoint is defective specking product; Often put a slice gold mark pad, " GO " key of pressing on a control panel is once (a slice) of specking; Specking finishes, and the gold mark pad of specking is placed in to room temperature natural drying 1 hour, on film, should can't see specking vestige.
The antibody chromatography membrane preparation method detecting for LH is as follows: get mouse-anti people LH antibody 500 ug, be added in 5 mL graduated centrifuge tubes, antibody diluent to 1 mL, Container Tag T mark.Get sheep anti-mouse igg antibody 25 μ L, be added in 5 mL graduated centrifuge tubes, antibody diluent to 1 mL, Container Tag C mark; The power supply of opening point film instrument, sets specking program, and specking amount is 1 μ L/cm, and No. 1 pipeline is for detecting band specking passage, and No. 2 pipelines are contrast band specking passage; No. 1 pipeline is placed in and detects band solution, No. 2 pipelines are placed in to contrast band solution, select initialize routine, 6 circulations of initialization; Chromatographic film is lain on a film instrument by fixed position, press " GO " key on control panel and start specking, after having put, take off, check the good chromatographic film of specking, detect band and contrast band and be two evenly, continuously and the straight line that connects whole chromatographic film be qualified specking product, in two straight lines, occur that breakpoint is defective specking product; Often put a slice chromatographic film, " GO " key of pressing on a control panel is once (a slice) of specking; Specking finishes, and the chromatographic film of specking is placed in to room temperature natural drying 1 hour, on film, should can't see specking vestige.
The protection sheet of wider portion on base plate is removed, and the edge lower limb of protection sheet above, will pull the chromatographic film of line, is attached on base plate plate in C line mode up; Collaurum pad is attached to T line below, and a little contacts with NC film; Sample pad is attached to collaurum pad below, and a little contacts with collaurum pad; Then remove top protection sheet, adsorptive pads is attached to the top of NC film, a little contacts with NC film; Protection sheet and index strip paper are attached to the test strips outside assembling one by one, are assembled into kilocalorie.Connect cutting machine power supply, set the film program of cutting, setting cutting width is 4 mm; Kilocalorie certified products are kept flat in cutting machine platform track, face up, press " GO " key on guidance panel, start cutting; Often put a slice kilocalorie certified products, press on guidance panel " GO " key once, until cut all kilocalorie certified products.
PH test paper, sialidase Test paper, leukocyte esterase Test paper, the filter paper material of hydrogen peroxide Test paper and amine test paper is chromatographic paper and nylon membrane, the material that base plate uses is transparent PVC sheet.
PH test paper is made: it is 300 mg/L that filter paper is immersed in to methyl orange concentration, and bromcresol green concentration is in the solution of 200 mg/L, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm;
Sialidase Test paper is made: it is 200 mg/L that filter paper is immersed in to the chloro-3-indoles of the bromo-4-of 5-acetyl neuraminic acid salinity, NBT concentration is 400 mg/L, trehalose concentration is 2000 mg/L, the pH value of PBS damping fluid is 6.5, volumetric molar concentration is in the solution of 0.01 M, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm;
Leukocyte esterase Test paper is made: it is 300 mg/L that filter paper is immersed in to the chloro-3-indolyl acetic acid salinity of the bromo-4-of 5-, and sucrose concentration is in the solution of 2000 mg/L, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm;
Hydrogen peroxide Test paper is made: it is 2.0 × 10 that filter paper is immersed in to peroxidase concn
5u/L, the concentration of surfactant in solution is 0.2 %, the concentration of TMB in enzyme solutions is in the solution of 700 mg/L, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm;
Amine test paper is made: reagent piece A is immersed in filter paper in the solution that concentration of potassium hydroxide is 10000 mg/L, dries; Reagent piece B is 500 mg/L for nylon membrane is immersed in to bromcresol green concentration, in the solution that Triton X-100 is 0.2%, dries.By reagent piece A and B with being cut into cutting machine the square that the length of side is 4 mm after adhered by double sided plaster;
By the pH test paper of well cutting, sialidase Test paper, leukocyte esterase Test paper, any two kinds in hydrogen peroxide Test paper and amine test paper cover with level or the mode of built-in embedding is placed on transparent PVC base plate, and are set up in parallel on draw-in groove with LH immunochromatographydetecting detecting test strip.
Preparation Example 3
The antibody colloidal gold labeling method detecting for LH is for adding 200 μ L, and 3% sal tartari regulates the pH of 50 mL colloidal gold solutions; Under stirring condition, add gradually 0.15 mL, 6.6 mg/mL anti-LH antibody to be marked, in above-mentioned colloidal gold solution, mixes, and room temperature leaves standstill 30 min; 12.5 mL confining liquids are slowly added in above-mentioned colloidal gold solution, mix, room temperature leaves standstill 30 min; The centrifugal 1h of 8000 4 ℃ of g, removes supernatant, stays loose deposits; Each pipe precipitation is dissolved with 25 mL cleansing solutions, and the centrifugal 1h of 6,000 4 ℃ of g, removes supernatant, stays loose deposits; Each pipe precipitation is dissolved with 1.2 mL cleansing solutions, proceeds in 1.5 mL centrifuge tubes, and the centrifugal 1h of 4,500 4 ℃ of g, removes supernatant; All precipitations are resuspended with the resuspended liquid of 1 mL colloidal gold antibody, 4 ℃ of preservations.
The specking method of the antibody colloidal gold pad detecting for LH is as follows: use the resuspended liquid of colloidal gold antibody by 4 times of the colloidal gold antibody bond dilutions of above-mentioned preparation; The power supply of opening point film instrument, sets specking program, and specking amount is 8 μ L/cm; No. 1 pipeline is specking passage; No. 1 pipeline is placed in to the resuspended solution of colloidal gold antibody, selects initialize routine, 6 circulations of initialization; Gold mark pad is lain on a film instrument by fixed position, press " GO " key on control panel and start specking, after having put, take off, check the good gold mark pad of specking, the colloidal gold antibody band of specking evenly, continuously and the straight line that connects whole gold mark pad be qualified specking product, in two straight lines, occur that breakpoint is defective specking product; Often put a slice gold mark pad, " GO " key of pressing on a control panel is once (a slice) of specking; Specking finishes, and the gold mark pad of specking is placed in to room temperature natural drying 1 hour, on film, should can't see specking vestige.
The antibody chromatography membrane preparation method detecting for LH is as follows: get mouse-anti people LH antibody 500 ug, be added in 5 mL graduated centrifuge tubes, antibody diluent to 1 mL, Container Tag T mark.Get sheep anti-mouse igg antibody 25 μ L, be added in 5 mL graduated centrifuge tubes, antibody diluent to 1 mL, Container Tag C mark; The power supply of opening point film instrument, sets specking program, and specking amount is 1 μ L/cm, and No. 1 pipeline is for detecting band specking passage, and No. 2 pipelines are contrast band specking passage; No. 1 pipeline is placed in and detects band solution, No. 2 pipelines are placed in to contrast band solution, select initialize routine, 6 circulations of initialization; Chromatographic film is lain on a film instrument by fixed position, press " GO " key on control panel and start specking, after having put, take off, check the good chromatographic film of specking, detect band and contrast band and be two evenly, continuously and the straight line that connects whole chromatographic film be qualified specking product, in two straight lines, occur that breakpoint is defective specking product; Often put a slice chromatographic film, " GO " key of pressing on a control panel is once (a slice) of specking; Specking finishes, and the chromatographic film of specking is placed in to room temperature natural drying 1 hour, on film, should can't see specking vestige.
The protection sheet of wider portion on base plate is removed, and the edge lower limb of protection sheet above, will pull the chromatographic film of line, is attached on base plate plate in C line mode up; Collaurum pad is attached to T line below, and a little contacts with NC film; Sample pad is attached to collaurum pad below, and a little contacts with collaurum pad; Then remove top protection sheet, adsorptive pads is attached to the top of NC film, a little contacts with NC film; Protection sheet and index strip paper are attached to the test strips outside assembling one by one, are assembled into kilocalorie.Connect cutting machine power supply, set the film program of cutting, setting cutting width is 4 mm; Kilocalorie certified products are kept flat in cutting machine platform track, face up, press " GO " key on guidance panel, start cutting; Often put a slice kilocalorie certified products, press on guidance panel " GO " key once, until cut all kilocalorie certified products.
PH test paper, sialidase Test paper, leukocyte esterase Test paper, the filter paper material of hydrogen peroxide Test paper and amine test paper is chromatographic paper and nylon membrane, the material that base plate uses is transparent PVC sheet.
PH test paper is made: it is 300 mg/L that filter paper is immersed in to methyl orange concentration, and bromcresol green concentration is in the solution of 200 mg/L, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm;
Sialidase Test paper is made: it is 200 mg/L that filter paper is immersed in to the chloro-3-indoles of the bromo-4-of 5-acetyl neuraminic acid salinity, NBT concentration is 400 mg/L, trehalose concentration is 2000 mg/L, the pH value of PBS damping fluid is 6.5, volumetric molar concentration is in the solution of 0.01 M, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm;
Leukocyte esterase Test paper is made: it is 300 mg/L that filter paper is immersed in to the chloro-3-indolyl acetic acid salinity of the bromo-4-of 5-, and sucrose concentration is in the solution of 2000 mg/L, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm;
Hydrogen peroxide Test paper is made: it is 2.0 × 10 that filter paper is immersed in to peroxidase concn
5u/L, the concentration of surfactant in solution is 0.2 %, the concentration of TMB in enzyme solutions is in the solution of 700 mg/L, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm;
Amine test paper is made: reagent piece A is immersed in filter paper in the solution that concentration of potassium hydroxide is 10000 mg/L, dries; Reagent piece B is 500 mg/L for nylon membrane is immersed in to bromcresol green concentration, in the solution that Triton X-100 is 0.2%, dries.By reagent piece A and B with being cut into cutting machine the square that the length of side is 4 mm after adhered by double sided plaster;
By the pH test paper of well cutting, sialidase Test paper, leukocyte esterase Test paper, in hydrogen peroxide Test paper and amine test paper, any three kinds cover with level or the mode of built-in embedding is placed on transparent PVC base plate, and are placed in respectively the upper and lower surface of base plate with LH immunochromatographydetecting detecting test strip.
Preparation Example 4
The antibody colloidal gold labeling method detecting for LH is for adding 200 μ L, and 3% sal tartari regulates the pH of 50 mL colloidal gold solutions; Under stirring condition, add gradually 0.15 mL, 6.6 mg/mL anti-LH antibody to be marked, in above-mentioned colloidal gold solution, mixes, and room temperature leaves standstill 30 min; 12.5 mL confining liquids are slowly added in above-mentioned colloidal gold solution, mix, room temperature leaves standstill 30 min; The centrifugal 1h of 8000 4 ℃ of g, removes supernatant, stays loose deposits; Each pipe precipitation is dissolved with 25 mL cleansing solutions, and the centrifugal 1h of 6,000 4 ℃ of g, removes supernatant, stays loose deposits; Each pipe precipitation is dissolved with 1.2 mL cleansing solutions, proceeds in 1.5 mL centrifuge tubes, and the centrifugal 1h of 4,500 4 ℃ of g, removes supernatant; All precipitations are resuspended with the resuspended liquid of 1 mL colloidal gold antibody, 4 ℃ of preservations.
The specking method of the antibody colloidal gold pad detecting for LH is as follows: use the resuspended liquid of colloidal gold antibody by 4 times of the colloidal gold antibody bond dilutions of above-mentioned preparation; The power supply of opening point film instrument, sets specking program, and specking amount is 8 μ L/cm; No. 1 pipeline is specking passage; No. 1 pipeline is placed in to the resuspended solution of colloidal gold antibody, selects initialize routine, 6 circulations of initialization; Gold mark pad is lain on a film instrument by fixed position, press " GO " key on control panel and start specking, after having put, take off, check the good gold mark pad of specking, the colloidal gold antibody band of specking evenly, continuously and the straight line that connects whole gold mark pad be qualified specking product, in two straight lines, occur that breakpoint is defective specking product; Often put a slice gold mark pad, " GO " key of pressing on a control panel is once (a slice) of specking; Specking finishes, and the gold mark pad of specking is placed in to room temperature natural drying 1 hour, on film, should can't see specking vestige.
The antibody chromatography membrane preparation method detecting for LH is as follows: get mouse-anti people LH antibody 500 ug, be added in 5 mL graduated centrifuge tubes, antibody diluent to 1 mL, Container Tag T mark.Get sheep anti-mouse igg antibody 25 μ L, be added in 5 mL graduated centrifuge tubes, antibody diluent to 1 mL, Container Tag C mark; The power supply of opening point film instrument, sets specking program, and specking amount is 1 μ L/cm, and No. 1 pipeline is for detecting band specking passage, and No. 2 pipelines are contrast band specking passage; No. 1 pipeline is placed in and detects band solution, No. 2 pipelines are placed in to contrast band solution, select initialize routine, 6 circulations of initialization; Chromatographic film is lain on a film instrument by fixed position, press " GO " key on control panel and start specking, after having put, take off, check the good chromatographic film of specking, detect band and contrast band and be two evenly, continuously and the straight line that connects whole chromatographic film be qualified specking product, in two straight lines, occur that breakpoint is defective specking product; Often put a slice chromatographic film, " GO " key of pressing on a control panel is once (a slice) of specking; Specking finishes, and the chromatographic film of specking is placed in to room temperature natural drying 1 hour, on film, should can't see specking vestige.
The protection sheet of wider portion on base plate is removed, and the edge lower limb of protection sheet above, will pull the chromatographic film of line, is attached on base plate plate in C line mode up; Collaurum pad is attached to T line below, and a little contacts with NC film; Sample pad is attached to collaurum pad below, and a little contacts with collaurum pad; Then remove top protection sheet, adsorptive pads is attached to the top of NC film, a little contacts with NC film; Protection sheet and index strip paper are attached to the test strips outside assembling one by one, are assembled into kilocalorie.Connect cutting machine power supply, set the film program of cutting, setting cutting width is 4 mm; Kilocalorie certified products are kept flat in cutting machine platform track, face up, press " GO " key on guidance panel, start cutting; Often put a slice kilocalorie certified products, press on guidance panel " GO " key once, until cut all kilocalorie certified products.
PH test paper, sialidase Test paper, leukocyte esterase Test paper, the filter paper material of hydrogen peroxide Test paper and amine test paper is chromatographic paper and nylon membrane, the material that base plate uses is transparent PVC sheet.
PH test paper is made: it is 300 mg/L that filter paper is immersed in to methyl orange concentration, and bromcresol green concentration is in the solution of 200 mg/L, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm;
Sialidase Test paper is made: it is 200 mg/L that filter paper is immersed in to the chloro-3-indoles of the bromo-4-of 5-acetyl neuraminic acid salinity, NBT concentration is 400 mg/L, trehalose concentration is 2000 mg/L, the pH value of PBS damping fluid is 6.5, volumetric molar concentration is in the solution of 0.01 M, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm;
Leukocyte esterase Test paper is made: it is 300 mg/L that filter paper is immersed in to the chloro-3-indolyl acetic acid salinity of the bromo-4-of 5-, and sucrose concentration is in the solution of 2000 mg/L, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm;
Hydrogen peroxide Test paper is made: it is 2.0 × 10 that filter paper is immersed in to peroxidase concn
5u/L, the concentration of surfactant in solution is 0.2 %, the concentration of TMB in enzyme solutions is in the solution of 700 mg/L, after drying, uses cutting machine to be cut into the square that the length of side is 4 mm;
Amine test paper is made: reagent piece A is immersed in filter paper in the solution that concentration of potassium hydroxide is 10000 mg/L, dries; Reagent piece B is 500 mg/L for nylon membrane is immersed in to bromcresol green concentration, in the solution that Triton X-100 is 0.2%, dries.By reagent piece A and B with being cut into cutting machine the square that the length of side is 4 mm after adhered by double sided plaster;
By the pH test paper of well cutting, sialidase Test paper, leukocyte esterase Test paper, any four kinds in hydrogen peroxide Test paper and amine test paper cover with level or the mode of built-in embedding is placed on transparent PVC base plate, and are set up in parallel on dismountable draw-in groove with LH immunochromatographydetecting detecting test strip.
Claims (2)
1. an interstitialcellstimulating hormone (ICSH) and bacterial vaginitis joint inspection kit, comprise the first base plate (10), it is characterized in that: in the one side of the first base plate (10), be provided with chromatographic film (6), the front end of chromatographic film (6) arranges opaque diaphragm (3), and the front end of opaque diaphragm (3) arranges arrow (2); The front end of arrow (2) arranges pad (1); In chromatographic film (6), be also disposed with detection line (4) and nature controlling line (5), chromatographic film (6) rear end is provided with adsorptive pads (7); Also comprise infection index detection zone (8);
Described infection index detection zone (8) is attached to the second base plate (11) above or is compound on any one surface of the first base plate (10);
Infection index detection zone is provided with LH immunochromatographydetecting detecting test strip in (8), and pH test paper, sialidase Test paper, leukocyte esterase Test paper, one or more in hydrogen peroxide Test paper and amine test paper.
2. an interstitialcellstimulating hormone (ICSH) and bacterial vaginitis joint inspection kit, comprise the first base plate (10), it is characterized in that: in the one side of the first base plate (10), be provided with chromatographic film (6), the front end of chromatographic film (6) arranges pad (1), and the front of pad (1) also arranges sample pad (9); In chromatographic film (6), be also disposed with detection line (4) and nature controlling line (5), chromatographic film (6) rear end is provided with adsorptive pads (7); On the lower surface of the first base plate (10), be also provided with infection index detection zone (8);
Infection index detection zone is provided with LH immunochromatographydetecting detecting test strip in (8), and pH test paper, sialidase Test paper, leukocyte esterase Test paper, one or more in hydrogen peroxide Test paper and amine test paper.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320690827.0U CN203616314U (en) | 2013-11-04 | 2013-11-04 | LH (luteinizing hormone) and BV (bacterial vaginitis) combined detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320690827.0U CN203616314U (en) | 2013-11-04 | 2013-11-04 | LH (luteinizing hormone) and BV (bacterial vaginitis) combined detection kit |
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| Publication Number | Publication Date |
|---|---|
| CN203616314U true CN203616314U (en) | 2014-05-28 |
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| CN201320690827.0U Expired - Fee Related CN203616314U (en) | 2013-11-04 | 2013-11-04 | LH (luteinizing hormone) and BV (bacterial vaginitis) combined detection kit |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108330159A (en) * | 2018-03-15 | 2018-07-27 | 深圳市瀚德标检生物工程有限公司 | A kind of detection reagent card and kit |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108330159A (en) * | 2018-03-15 | 2018-07-27 | 深圳市瀚德标检生物工程有限公司 | A kind of detection reagent card and kit |
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