CN204666637U - Mycoplasma pneumoniae IgM, IgG, IgA tri-test card - Google Patents
Mycoplasma pneumoniae IgM, IgG, IgA tri-test card Download PDFInfo
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- CN204666637U CN204666637U CN201520364385.XU CN201520364385U CN204666637U CN 204666637 U CN204666637 U CN 204666637U CN 201520364385 U CN201520364385 U CN 201520364385U CN 204666637 U CN204666637 U CN 204666637U
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Abstract
The utility model provides a kind of mycoplasma pneumoniae IgM, IgG, IgA tri-test card, described test card comprises test strips and box body, described test strips comprises base plate and is connected with the order head and the tail be arranged on base plate and there is the superimposed sample pad of part, labeling pad, reaction film and adsorptive pads, described labeling pad is adsorbed with the colloid gold particle of mark mycoplasma pneumoniae p1 protein, described reaction film is provided with three detection lines and a control line, described three detection lines are respectively by mouse-anti people IgM, mouse-anti human IgG, the thin film wire that mouse-anti people IgA antibody is printed, described control line is the thin film wire printed by sheep anti-mouse igg, described three detection lines are parallel to each other with a control line and vertical with sample flow direction, described box body is provided with the well corresponding with described sample pad location, the view window corresponding with described three detection lines and a control line.The utility model is easy to use, and cost is low.
Description
Technical field
The utility model belongs to immunochemistry rapid detection technical field, is specifically related to a kind of three test card that can detect mycoplasma pneumoniae IgM, IgG, IgA.
Background technology
Mycoplasma pneumoniae (Mycoplasma pneumoniae, MP) is the pathogen causing pneumonia more common, and the pneumonia of about 10-20% all caused by mycoplasma infection.Mycoplasma pneumoniae, mainly through respiratory infectious, also can cause pharyngitis, bronchitis, and severe patient can cause the multiple extrapulmonary complications such as encephalitis, ephritis, myocarditis simultaneously, and the incidence of disease is the highest with teenager, should find early and treat.
After mycoplasma pneumoniae infection, in serum, there is specific IgM and IgA antibody the earliest, after a few week, occur specific IgG antibodies.Therefore the detection of MP-IgM antibody-like be early diagnosis MP relevant disease most important be also topmost means.But specificity MP-IgM can disappear after antibody produces a few weeks or months, therefore detect merely the false negative that MP-IgM can exist certain probability.Thus the detection infecting the IgG antibody that the later stage produces also is the important means of MP diagnosis and a necessary ring.In addition, although holding time of IgA appearance is short, it also can as an important supplement of mycoplasma pneumoniae diagnosis.
Current existing discriminating MP means (as cultivation, PCR, ELISA etc.) complex operation, waste time and energy and need specialized equipment, be thus unfavorable for clinical widespread use.Colloidal gold immunity chromatography is short for detection time, good stability, easy and simple to handle, without the need to other instrument and equipments, is applicable to on-the-spot quick diagnosis, but there is no three test card simultaneously detecting mycoplasma pneumoniae IgM, IgG, IgA at present.
Utility model content
The purpose of this utility model is to provide a kind of test card that simultaneously can detect mycoplasma pneumoniae IgM, IgG, IgA tri-kinds of antibody, and cost is low, easy and simple to handle, overcome prior art can only one by one individual event detect defect.
The technical solution of the utility model is:
A kind of mycoplasma pneumoniae IgM, IgG, IgA tri-test card, described test card comprises test strips and box body, described test strips comprises base plate and is connected with the order head and the tail be arranged on base plate and there is the superimposed sample pad of part, labeling pad, reaction film and adsorptive pads, described labeling pad is adsorbed with the colloid gold particle of mark mycoplasma pneumoniae p1 protein, described reaction film is provided with three detection lines and a control line, described three detection lines are respectively by mouse-anti people IgM, mouse-anti human IgG, the thin film wire that mouse-anti people IgA antibody is printed, described control line is the thin film wire printed by sheep anti-mouse igg, described three detection lines are parallel to each other with a control line and vertical with sample flow direction, described box body is provided with the well corresponding with described sample pad location, the view window corresponding with described three detection lines and a control line.
Described base plate is PVC base plate, and described sample pad is glass cotton pad, and described adsorptive pads is thieving paper, and described labeling pad is glass fibre, and described reaction film is nitrocellulose filter.
The colloid gold particle of described mark mycoplasma pneumoniae p1 protein is arranged at described labeling pad surface by spraying.
Described box body is rigid plastic.
Described box body is provided with test item information window in its position corresponding with described adsorptive pads, for arranging test item explanation.
The utility model has following advantage:
(1) detection specificity is high, can detect mycoplasma pneumoniae three kinds of antibody, for clinical diagnosis provides better reference simultaneously.
(2) easy and simple to handle, detection time is short, and an application of sample can realize the detection of higher flux, overcomes the shortcoming that conventional individual event test card flux is low, improves detection efficiency.
(3) result is visual in image, is convenient to observe, and the color change by means of only detection line and nature controlling line gets final product sentence read result.
(4) testing cost is low, does not need specific apparatus, applied range.
Accompanying drawing explanation
Fig. 1 is the one-piece construction schematic diagram of the utility model embodiment; Wherein, 1, well; 2, view window; 3, test item information window.
Fig. 2 is the cut-open view of the test strips of the utility model embodiment, wherein, and 4, base plate; 5, application of sample pad; 6, labeling pad; 7, reaction film; 8, adsorptive pads.
Embodiment
The present embodiment provides a kind of mycoplasma pneumoniae IgM, IgG, IgA tri-test card, it forms by test strips with for the box body of accommodating test strips, described test strips comprises base plate 4 and is connected with the order head and the tail be arranged on base plate 4 and there is the superimposed sample pad of part 5, labeling pad 6, reaction film 7 and adsorptive pads 8, described labeling pad is adsorbed with the colloid gold particle of mark mycoplasma pneumoniae p1 protein, described reaction film is provided with three detection lines 12, 13, 14 and a control line 11, described three detection lines 12, 13, 14 are respectively by mouse-anti people IgM, mouse-anti human IgG, the thin film wire that mouse-anti people IgA antibody is printed, described control line 11 is the thin film wire printed by sheep anti-mouse igg, described three detection line 12-14 are parallel to each other with a control line 11 and vertical with sample flow direction, described box body is provided with the well 1 corresponding with described sample pad location, the view window 2 corresponding with described three detection line 12-14 and control lines 11.
The preparation of three test card of the present embodiment: mycoplasma pneumoniae p1 protein is marked at gold nano grain surface by the mode of physisorption, then be sprayed in labeling pad 6, carry out frozen dried.Respectively sheep anti-mouse igg, mouse-anti people IgM, mouse-anti human IgG, mouse-anti people IgA antibody are printed on reaction film again, successively formation control line 11 and three detection line 12-14, carry out drying process.Base plate 4, sample pad 5, adsorptive pads 8 and the labeling pad 6 prepared and reaction film 7 are assembled into test strips (as shown in Figure 2), then enclose in box body, namely form test card (as shown in Figure 1) described in the present embodiment.
The detection of sample: drip 2 and bleed clearly (about 80 μ L) in well, then drip 3 cleansing solutions in well 1, observations in 10 minutes after liquid fully sucks.
Result judges: if only have control line 11 to show a brownish red response line, then represent that mycoplasma pneumoniae IgM, IgG, IgA testing result is feminine gender; If when 11 and 12 liang of place's display brownish red response lines, then represent that mycoplasma pneumoniae IgM testing result is positive; If when 11 and 13 liang of place's display brownish red response lines, then represent that mycoplasma pneumoniae IgG testing result is positive; If when 11 and 14 liang of place's display brownish red response lines, then represent that mycoplasma pneumoniae IgA testing result is positive; If when 11,12 and 13 3 place's display brownish red response line, then represent that mycoplasma pneumoniae IgM, IgG testing result is positive; If when 11,12 and 14 3 place's display brownish red response line, then represent that mycoplasma pneumoniae IgM, IgA testing result is positive; If when 11,13 and 14 3 place's display brownish red response line, then represent that mycoplasma pneumoniae IgG, IgA testing result is positive; If when 11,12,13 and 14 show brownish red response line everywhere, then represent that mycoplasma pneumoniae IgM, IgG, IgA testing result is the positive; If at 11 places without the display of brownish red response line, then represent that test card is invalid.
Claims (5)
1. a mycoplasma pneumoniae IgM, IgG, IgA tri-test card, described test card comprises test strips and box body, described test strips comprises base plate and is connected with the order head and the tail be arranged on base plate and there is the superimposed sample pad of part, labeling pad, reaction film and adsorptive pads, it is characterized in that: described labeling pad is adsorbed with the colloid gold particle of mark mycoplasma pneumoniae p1 protein, described reaction film is provided with three detection lines and a control line, described three detection lines are respectively by mouse-anti people IgM, mouse-anti human IgG, the thin film wire that mouse-anti people IgA antibody is printed, described control line is the thin film wire printed by sheep anti-mouse igg, described three detection lines are parallel to each other with a control line and vertical with sample flow direction, described box body is provided with the well corresponding with described sample pad location, the view window corresponding with described three detection lines and a control line.
2. mycoplasma pneumoniae IgM, IgG, IgA according to claim 1 tri-test card, it is characterized in that: described base plate is PVC base plate, described sample pad is glass cotton pad, and described adsorptive pads is thieving paper, described labeling pad is glass fibre, and described reaction film is nitrocellulose filter.
3. mycoplasma pneumoniae IgM, IgG, IgA according to claim 1 tri-test card, is characterized in that: the colloid gold particle of described mark mycoplasma pneumoniae p1 protein is arranged at described labeling pad surface by spraying.
4. mycoplasma pneumoniae IgM, IgG, IgA according to claim 1 tri-test card, is characterized in that: described box body is rigid plastic.
5. mycoplasma pneumoniae IgM, IgG, IgA according to claim 1 tri-test card, is characterized in that: described box body is provided with test item information window in its position corresponding with described adsorptive pads, for arranging test item explanation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520364385.XU CN204666637U (en) | 2015-05-29 | 2015-05-29 | Mycoplasma pneumoniae IgM, IgG, IgA tri-test card |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520364385.XU CN204666637U (en) | 2015-05-29 | 2015-05-29 | Mycoplasma pneumoniae IgM, IgG, IgA tri-test card |
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| CN204666637U true CN204666637U (en) | 2015-09-23 |
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| CN201520364385.XU Active CN204666637U (en) | 2015-05-29 | 2015-05-29 | Mycoplasma pneumoniae IgM, IgG, IgA tri-test card |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105823875A (en) * | 2016-03-15 | 2016-08-03 | 威尚生物技术（合肥）有限公司 | Mycoplasma pneumoniae antibody immunity colloid gold test strip and preparation method thereof |
| CN110095601A (en) * | 2019-05-16 | 2019-08-06 | 北京标驰泽惠生物科技有限公司 | The quantitative detecting method and kit of Porcine epidemic diarrhea virus Specific IgA antibody in a kind of sow colostrum |
| CN110133255A (en) * | 2018-02-02 | 2019-08-16 | 中国人民解放军军事科学院军事医学研究院 | A kind of quickly SERS- immunochromatography detection method with highly sensitive detection mycoplasma pneumoniae infection |
| CN111505313A (en) * | 2020-05-07 | 2020-08-07 | 厦门万渤生物技术有限公司 | Diagnostic kit for combined diagnosis of mycoplasma pneumonia and application thereof |
| CN111562365A (en) * | 2020-05-29 | 2020-08-21 | 浙江省人民医院 | Novel coronavirus IgG/IgA antibody detection kit |
-
2015
- 2015-05-29 CN CN201520364385.XU patent/CN204666637U/en active Active
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105823875A (en) * | 2016-03-15 | 2016-08-03 | 威尚生物技术（合肥）有限公司 | Mycoplasma pneumoniae antibody immunity colloid gold test strip and preparation method thereof |
| CN110133255A (en) * | 2018-02-02 | 2019-08-16 | 中国人民解放军军事科学院军事医学研究院 | A kind of quickly SERS- immunochromatography detection method with highly sensitive detection mycoplasma pneumoniae infection |
| CN110095601A (en) * | 2019-05-16 | 2019-08-06 | 北京标驰泽惠生物科技有限公司 | The quantitative detecting method and kit of Porcine epidemic diarrhea virus Specific IgA antibody in a kind of sow colostrum |
| CN111505313A (en) * | 2020-05-07 | 2020-08-07 | 厦门万渤生物技术有限公司 | Diagnostic kit for combined diagnosis of mycoplasma pneumonia and application thereof |
| CN111505313B (en) * | 2020-05-07 | 2022-07-05 | 厦门万渤生物技术有限公司 | Diagnostic kit for combined diagnosis of mycoplasma pneumonia and application thereof |
| CN111562365A (en) * | 2020-05-29 | 2020-08-21 | 浙江省人民医院 | Novel coronavirus IgG/IgA antibody detection kit |
| CN111562365B (en) * | 2020-05-29 | 2024-01-05 | 浙江省人民医院 | Novel coronavirus IgG/IgA antibody detection kit |
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