CN201852840U - Test paper card for quickly diagnosing human echinococcosis adopting colloid gold immunochromatography to test urine - Google Patents
Test paper card for quickly diagnosing human echinococcosis adopting colloid gold immunochromatography to test urine Download PDFInfo
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- CN201852840U CN201852840U CN2010202803685U CN201020280368U CN201852840U CN 201852840 U CN201852840 U CN 201852840U CN 2010202803685 U CN2010202803685 U CN 2010202803685U CN 201020280368 U CN201020280368 U CN 201020280368U CN 201852840 U CN201852840 U CN 201852840U
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- echinococcosis
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Abstract
The utility model discloses a test paper card for quickly diagnosing human echinococcosis adopting colloid gold immunochromatography to test urine. The test paper card comprises a plastic card box and a detection module in the card box, wherein the plastic card box is provided with a sample feeding hole and an observation window; the detection model comprises filter sample paper, a nitrocellulose membrane and water-absorbing paper; the water-absorbing paper and the nitrocellulose membrane are absorbed with an echinococcosi-resistant human source single chain variable regions allantoic liquid antigen marked by colloid gold; and a quality control line and a detection line are marked on the echinococcosi-resistant human source single chain variable regions marked by the colloid gold on the nitrocellulose membrane; the filter sample paper faces to the sample feeding hole; and the nitrocellulose membrane faces to the observation window. The test paper card is simple, convenient, fast, reliable and stable and has no wound.
Description
Technical field
The utility model relates to the medical biotechnology field, and particularly a kind of Urine test paper of quick diagnosis human body echinococcosis can utilize the observable change color diagnosis of naked eyes whether to infect echinococcosis fast.
Background technology
Echinococcosis has the onset concealment as a kind of zoonosis of serious harm human health, and slower development recurs frequent characteristics, needs early diagnosis and detection regularly.Popular serious at China's zoonosis, the Ministry of Public Health proposes the target of " to reaching district occurred frequently 60% population coverage in the echinococcosis epidemiology survey task " in April, 2007.Community hospital, epidemic prevention station, medical colleges and schools and scientific research institution need special scientific research apparatus that this disease is carried out serology, immunology and imaging diagnosis but the field investigation of popular district and grass-roots unit lack special instrument and equipment personnel, cause a lot of cases can not early find early to diagnose early treatment.
Summary of the invention
The purpose of this utility model is to overcome weak point of the prior art, and the utility model provides a kind of human body echinococcosis colloidal gold immunochromatographimethod examination urine fast diagnose test paper card based on the immune response device of colloidal gold immunochromatographimethod detection principle.
A kind of human body echinococcosis colloidal gold immunochromatographimethod examination urine fast diagnose test paper card of the present utility model, it comprises the detection module in plastics cartridge and the cartridge; On the described plastics cartridge well and view window are arranged; Described detection module comprises filter sample paper, nitrocellulose membrane and thieving paper; Be adsorbed with strand variable region, the anti-echinococcosis cyst fluid antigen people source antibody of colloid gold label on described thieving paper and the nitrocellulose membrane; Colloid gold label on the described nitrocellulose membrane has on the antibody of strand variable region, anti-echinococcosis cyst fluid antigen people source and is marked with a nature controlling line and a detection line; Described filter sample paper is facing to well, and described nitrocellulose filter is facing to view window.
Described view window is a rectangle.
Described detection line is by marked by streptavidin.
Described nature controlling line is by the rabbit anti-mouse igg mark.
The utlity model has following advantage:
Utilize the Protocols in Molecular Biology development to have easy (without instrument and equipment, do not need the professional and technical personnel to operate, can use in patient family), fast (finishing analysiss within 1-5 minute), do not have to create and (in urine, test, do not need collecting blood sample), the reliable external quick diagnosis reagent kit of the human body echinococcosis of (but quantitative interpretation result, diagnostic accordance rate reaches more than 90%), stable (product is being stablized more than a year under the regulation preservation condition).The specificity of Echinococcus hydatid cyst antibody and susceptibility have determined the sensitivity and the specificity of this diagnostic tool.Employing, can reduce the cross reaction with other diseases, thereby improve sensitivity and specificity as the antibody of catching Echinococcus hydatid cyst antigen at strand variable region, the people source antibody of the reorganization holoprotein of echinococcosis cyst fluid antigen B, has reduced and has failed to pinpoint a disease in diagnosis or mistaken diagnosis.
Description of drawings
Fig. 1 is the structural representation of embodiment of the present utility model.
Fig. 2 is the external structure synoptic diagram of Fig. 1.
Embodiment
Further the utility model is described by the following examples:
With reference to figure 1, Fig. 2, pick-up unit: be made up of the plastics cartridge of outside and the detection module of the inside, outside plastics cartridge comprises base 1 and shell 2, and well 3 and rectangle view window 4 are arranged on the shell 2; Inner detection module is made up of filter sample paper 5, nitrocellulose membrane 6, thieving paper 7.Strand variable region, the anti-echinococcosis cyst fluid antigen people source antibody 8 of colloid gold label is adsorbed on thieving paper 7 and the nitrocellulose membrane 6, forms a nature controlling line 9 and a detection line 10; Filter sample paper 5 is over against well 3, and nitrocellulose membrane 6 is over against view window 4; Detection line 10 is by marked by streptavidin; Nature controlling line 9 is by the rabbit anti-mouse igg mark.
Testing process: urine sample enters behind the well because capillarity is mobile to the thieving paper direction, middle through bag in advance by the zone of strand variable region, anti-echinococcosis cyst fluid antigen people source antibody of colloid gold label, golden label is redissolved fully, antigen and golden labelled antibody form golden labelled antibody-antigenic compound, continue to flow to forward on the nitrocellulose membrane, be fixed with the detection line place of capture antigen.When the result is positive, on golden labelled antibody-antigenic compound, will form golden labelled antibody-antigen-insolubilized antibody compound, present red positive band; As there not being antigen in the sample, then be not hunted down, just do not develop the color, then the result is negative.Unnecessary free gold labelled antibody continues to move forward to and is fixed with anti-golden marked body two anti-places, and being fixed presents red nature controlling line.
Make raw material: gold chloride: Beijing chemical reagent factory; Streptavidin: BNHS:Sigma; Strand variable region, anti-echinococcosis cyst fluid antigen people source antibody: (GENE BANK:AF407670), provide by Zhejiang University, can purchase, or Zhejiang University provides in Zhejiang University; Nitrocellulose membrane: Sigma; Rabbit anti-mouse igg, Sigma.
Preparation of Colloidal Gold and mark (routine techniques): tri-distilled water dissolved chlorine auric acid, be heated to boiling, to final concentration be 0.01%, every 100ml adds 10% trisodium citrate aqueous solution 1.5ml.Boil 10min again and be orange red, use 0.2mol/LK after the cooling
2CO
3Transfer to pH8.5, stir the anti-echinococcosis cyst fluid antigen people source strand variable region antibody 2mg that adds purifying down fast, add the protein 25 0mg of cow's serum, stir 10min fast, add 10%NaCl, the concentration that makes NaCl is 1%, shake up, respectively with 2000,4000, the 10000r/min gradient centrifugation, 10min/ time, obtain the antibody conjugates that sediment is the golden mark of preliminary purification at last, be dissolved in storage liquid.
Cellulose nitrate membrane marker (routine techniques): the nitrocellulose membrane bag is by strand variable region, the anti-echinococcosis cyst fluid antigen people source antibody of colloid gold label, 1: 100 Streptavidin (detection line) of mark and rabbit anti-mouse igg (control line) on the nitrocellulose membrane, it is wide to be 1mm.Drying the back seals with the 0.1mol/LpH7.4PBS that contains 1% NBCS.Be bonded at successively on the white plastic sheet after the drying.Be cut into 0.6cm * 10cm bar ,-4 ℃ store for future use.
The result judges: 1 redness of position T appearance with detection line on the nitrocellulose membrane is the Echinococcus hydatid cyst positive; If have only the nature controlling line redness negative; Article two, line does not all have colour developing and is test-strips inefficacy.
At last, it is also to be noted that what more than enumerate only is specific embodiment of the utility model.Obviously, the utility model is not limited to above examples of implementation, and many distortion can also be arranged.All distortion that those of ordinary skill in the art can directly derive or associate from the disclosed content of the utility model all should be thought protection domain of the present utility model.
Claims (4)
1. a human body echinococcosis colloidal gold immunochromatographimethod tries urine fast diagnose test paper card, and it is characterized in that: it comprises the detection module in plastics cartridge and the cartridge; On the described plastics cartridge well and view window are arranged; Described detection module comprises filter sample paper, nitrocellulose membrane and thieving paper; Be adsorbed with strand variable region, the anti-echinococcosis cyst fluid antigen people source antibody of colloid gold label on described thieving paper and the nitrocellulose membrane; Colloid gold label on the described nitrocellulose membrane has on the antibody of strand variable region, anti-echinococcosis cyst fluid antigen people source and is marked with a nature controlling line and a detection line; Described filter sample paper is facing to well, and described nitrocellulose filter is facing to view window.
2. according to the described test card of claim 1, it is characterized in that: described view window is a rectangle.
3. according to the described test card of claim 1, it is characterized in that: described detection line is by marked by streptavidin.
4. according to the described test card of claim 1, it is characterized in that: described nature controlling line is by the rabbit anti-mouse igg mark.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010202803685U CN201852840U (en) | 2010-08-04 | 2010-08-04 | Test paper card for quickly diagnosing human echinococcosis adopting colloid gold immunochromatography to test urine |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010202803685U CN201852840U (en) | 2010-08-04 | 2010-08-04 | Test paper card for quickly diagnosing human echinococcosis adopting colloid gold immunochromatography to test urine |
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| Publication Number | Publication Date |
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| CN201852840U true CN201852840U (en) | 2011-06-01 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2010202803685U Expired - Lifetime CN201852840U (en) | 2010-08-04 | 2010-08-04 | Test paper card for quickly diagnosing human echinococcosis adopting colloid gold immunochromatography to test urine |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105785028A (en) * | 2012-08-10 | 2016-07-20 | 杭州华得森生物技术有限公司 | Rapid diagnosis kit for detecting novel human cardiac failure marker ST2 |
| CN105974114A (en) * | 2016-07-06 | 2016-09-28 | 聊城大学 | Nano-gold immunochromatography test paper capable of detecting chicken avian influenza and application thereof |
-
2010
- 2010-08-04 CN CN2010202803685U patent/CN201852840U/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105785028A (en) * | 2012-08-10 | 2016-07-20 | 杭州华得森生物技术有限公司 | Rapid diagnosis kit for detecting novel human cardiac failure marker ST2 |
| CN105974114A (en) * | 2016-07-06 | 2016-09-28 | 聊城大学 | Nano-gold immunochromatography test paper capable of detecting chicken avian influenza and application thereof |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20110601 |
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| CX01 | Expiry of patent term |