CN204359795U - Mite specific IgG4 subclass antibodies detection kit - Google Patents
Mite specific IgG4 subclass antibodies detection kit Download PDFInfo
- Publication number
- CN204359795U CN204359795U CN201420815685.0U CN201420815685U CN204359795U CN 204359795 U CN204359795 U CN 204359795U CN 201420815685 U CN201420815685 U CN 201420815685U CN 204359795 U CN204359795 U CN 204359795U
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- CN
- China
- Prior art keywords
- storage tank
- mite
- igg4 subclass
- subclass antibodies
- specific igg4
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- 238000001514 detection method Methods 0.000 title claims abstract description 16
- 210000002966 serum Anatomy 0.000 claims abstract description 43
- 239000013641 positive control Substances 0.000 claims abstract description 28
- 239000007788 liquid Substances 0.000 claims abstract description 10
- 239000013642 negative control Substances 0.000 claims abstract description 7
- 239000000243 solution Substances 0.000 claims abstract description 7
- 102000004190 Enzymes Human genes 0.000 claims abstract description 6
- 108090000790 Enzymes Proteins 0.000 claims abstract description 6
- 238000004140 cleaning Methods 0.000 claims abstract description 5
- UAIUNKRWKOVEES-UHFFFAOYSA-N 3,3',5,5'-tetramethylbenzidine Chemical compound CC1=C(N)C(C)=CC(C=2C=C(C)C(N)=C(C)C=2)=C1 UAIUNKRWKOVEES-UHFFFAOYSA-N 0.000 claims abstract description 4
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 claims abstract description 4
- 230000000172 allergic effect Effects 0.000 claims abstract description 4
- 208000010668 atopic eczema Diseases 0.000 claims abstract description 4
- 238000010790 dilution Methods 0.000 claims abstract description 4
- 239000012895 dilution Substances 0.000 claims abstract description 4
- 229940005654 nitrite ion Drugs 0.000 claims abstract description 4
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 4
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 4
- 238000002965 ELISA Methods 0.000 claims 1
- 238000000586 desensitisation Methods 0.000 abstract description 7
- 230000000694 effects Effects 0.000 abstract description 7
- 238000003556 assay Methods 0.000 abstract description 3
- 238000011156 evaluation Methods 0.000 abstract description 2
- 239000003547 immunosorbent Substances 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 abstract description 2
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 210000002381 plasma Anatomy 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- 108060003951 Immunoglobulin Proteins 0.000 description 2
- 102000018358 immunoglobulin Human genes 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 206010018910 Haemolysis Diseases 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000008588 hemolysis Effects 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 238000010166 immunofluorescence Methods 0.000 description 1
- 238000009169 immunotherapy Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- -1 nitrite ions Chemical class 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
Abstract
The utility model discloses a kind of mite specific IgG4 subclass antibodies detection kit, comprise box body, bag is provided with by the ELISA Plate storage tank of mite allergic protein in described box body, mite specific IgG4 subclass antibodies positive control serum storage tank, mite specific IgG4 subclass antibodies negative control sera storage tank, the mouse-anti human IgG 4 antibody storage tank of HRP mark, tmb substrate nitrite ion storage tank, enzyme labelled antibody dilution storage tank, serum samples diluted liquid storage tank, concentrated cleaning solution storage tank and reaction terminating liquid storage tank.The utility model provides a kind of mite specific IgG4 subclass antibodies detection kit on the basis of euzymelinked immunosorbent assay (ELISA), the content of the mite specific IgG4 subclass antibodies in the patient body that can detect fast, for mite desensitization treatment curative effect provides evaluation index; Highly sensitive, specificity is good, convenient and swift, and testing cost is low, is easy to promote.
Description
Technical field
The utility model relates to disease surveillance and diagnostic reagent research field, is specifically related to a kind of mite specific IgG4 subclass antibodies detection kit.
Background technology
Immunoglobulin (Ig) (IgG) is the immunoreactive main effects molecule of human body fluid, accounts for 75% of healthy individuals immunoglobulin (Ig) total amount.The IgG of human body has four kinds of hypotypes, is respectively IgG1, IgG2, IgG3, and IgG4, and the relative concentration of these four kinds of hypotypes in serum is: IgG1>IgG2>IgG3=IgG4.IgG hypotype defect or out of proportion be an immunoreactive feature.Some disease can cause the change of a certain hypotype content, even if clinically without obviously performance, serum total Ig G is also normal.Therefore measure the relative concentration of IgG hypotype, play an important role in specific active immunotherapy process.
As far back as 1998, the World Health Organization (WHO) just pointed out: desensitization treatment is the unique method of modified sensitivity disease condition development.After inoculation mite, the immunocyte of body is activated and produces immune response, and in patients serum, mite specific IgG4 subclass antibodies level can significantly rise, thus strengthens body resistance against diseases.The individual difference of the quality of mite, means of transportation, preservation condition, vaccine program and patient all may affect the effect of desensitization treatment.Therefore, detection is carried out to the curative effect of desensitization treatment significant, and be evaluate the important means of desensitization effect to the content detection of mite specific IgG 4 antibody.At present, due to the immuno-fluorescence assay that the method detecting mite specific IgG 4 antibody level is mainly external, because this detection method cost is too high, domestic various big hospital carries out the monitoring of desensitization treatment curative effect not yet on a large scale.Therefore, be necessary the basis to prior art is improved.
Summary of the invention
The purpose of this utility model is for the deficiencies in the prior art, provides a kind of highly sensitive, convenient and swift, mite specific IgG4 subclass antibodies detection kit that cost is low.
The utility model for the adopted technical scheme that solves the problem is: a kind of mite specific IgG4 subclass antibodies detection kit, comprise box body, be provided with bag in described box body by the mouse-anti human IgG 4 antibody storage tank of the ELISA Plate storage tank of mite allergic protein, mite specific IgG4 subclass antibodies positive control serum storage tank, mite specific IgG4 subclass antibodies negative control sera storage tank, HRP mark, tmb substrate nitrite ion storage tank, enzyme labelled antibody dilution storage tank, serum samples diluted liquid storage tank, concentrated cleaning solution storage tank and reaction terminating liquid storage tank.
Described mite specific IgG4 subclass antibodies positive control serum storage tank comprises the positive control serum storage area of concentration higher than 1ug/ml, the positive control serum storage area of concentration within the scope of 0.4-1ug/ml, the positive control serum storage area of concentration within the scope of 100-400ng/ml, the positive control serum storage area of concentration within the scope of 40-100ng/ml, the positive control serum storage area of concentration within the scope of 10-40ng/ml and concentration are less than the positive control serum storage area of 10ng/ml.
The utility model provides a kind of mite specific IgG4 subclass antibodies detection kit on the basis of euzymelinked immunosorbent assay (ELISA), adopt the control serum of six kinds of different contents, the content of the mite specific IgG4 subclass antibodies in the patient body that can detect rapidly and accurately, for mite desensitization treatment curative effect provides evaluation index; Highly sensitive, specificity is good, convenient and swift, and testing cost is low, is easy to promote.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model.
Embodiment
In order to better understand the utility model, below in conjunction with accompanying drawing, the utility model is further described.
A kind of mite specific IgG4 subclass antibodies detection kit as shown in Figure 1, comprise box body 1, be provided with bag in described box body 1 by the mouse-anti human IgG 4 antibody storage tank 5 of the ELISA Plate storage tank 2 of mite allergic protein, mite specific IgG4 subclass antibodies positive control serum storage tank 3, mite specific IgG4 subclass antibodies negative control sera storage tank 4, HRP mark, tmb substrate nitrite ion storage tank 6, enzyme labelled antibody dilution storage tank 7, serum samples diluted liquid storage tank 8, concentrated cleaning solution storage tank 9 and reaction terminating liquid storage tank 10.
Described mite specific IgG4 subclass antibodies positive control serum storage tank 3 comprises the positive control serum storage area 31 of concentration higher than 1ug/ml, the positive control serum storage area 32 of concentration within the scope of 0.4-1ug/ml, the positive control serum storage area 33 of concentration within the scope of 100-400ng/ml, the positive control serum storage area 34 of concentration within the scope of 40-100ng/ml, the positive control serum storage area 35 of concentration within the scope of 10-40ng/ml and concentration are less than the positive control serum storage area 36 of 10ng/ml.
Before detection, blood sample collection, and prepare serum or blood plasma (require serum or blood plasma limpid without haemolysis) according to a conventional method, with serum samples diluted liquid dilute serum or blood plasma in centrifuge tube, mixing, get 100 μ L dilute after serum sample detect; By all reagent and serum sample slowly balanced room temperature to 18-25 DEG C (reagent can not directly 37 DEG C of dissolvings), dilute 5 parts of mite specific IgG4 subclass antibodies positive control serums and 1 part of mite specific IgG4 subclass antibodies negative control sera on request, with mouse-anti human IgG 4 antibody that enzyme labelled antibody diluted HRP marks, and concentrated cleaning solution is diluted to working concentration.
Concrete detection method is as follows:
1, application of sample: take out ELISA Plate, if a negative control hole and five Positive control wells, the corresponding mite specific IgG4 subclass antibodies positive control serum 100 μ L adding mite specific IgG4 subclass antibodies negative control sera and variable concentrations respectively; Separately establish two blank well; All the other holes are the serum sample detect aperture of collecting clinically, and every hole adds serum sample 100 μ L;
2, hatch: under above each hole sealing is placed in 37 DEG C of constant incubators, hatch 30 minutes;
3, wash: every hole adds 300 μ L cleansing solutions, pats dry, repeated washing 4 times;
4, enzyme labelled antibody reaction: every hole adds mouse-anti human IgG 4 antibody of the HRP mark of 100 μ L, and sealing is placed on the constant incubator of 37 DEG C, hatches 30 minutes;
5, wash: every hole adds 300 μ L cleansing solutions, pats dry, repeated washing 4 times;
6, chromogenic reaction: every hole adds 100 μ LTMB substrate nitrite ions, and sealing is placed on 37 DEG C of constant incubators, lucifuge, reaction 15 reaction;
7, cessation reaction: every hole adds 50 μ L reaction terminating liquids, cessation reaction;
8, result judges: judge whether to create mite specific IgG4 subclass antibodies according to the color that every hole shows, the depth of color represent produce mite specific IgG4 subclass antibodies number.
Claims (2)
1. a mite specific IgG4 subclass antibodies detection kit, comprise box body, it is characterized in that, bag is provided with by the ELISA Plate storage tank of mite allergic protein in described box body, mite specific IgG4 subclass antibodies positive control serum storage tank, mite specific IgG4 subclass antibodies negative control sera storage tank, the mouse-anti human IgG 4 antibody storage tank of HRP mark, tmb substrate nitrite ion storage tank, enzyme labelled antibody dilution storage tank, serum samples diluted liquid storage tank, concentrated cleaning solution storage tank and reaction terminating liquid storage tank.
2. mite specific IgG4 subclass antibodies detection kit as claimed in claim 1, it is characterized in that, described mite specific IgG4 subclass antibodies positive control serum storage tank comprises the positive control serum storage area of concentration higher than 1ug/ml, the positive control serum storage area of concentration within the scope of 0.4-1ug/ml, the positive control serum storage area of concentration within the scope of 100-400ng/ml, the positive control serum storage area of concentration within the scope of 40-100ng/ml, the positive control serum storage area of concentration within the scope of 10-40ng/ml and concentration are less than the positive control serum storage area of 10ng/ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420815685.0U CN204359795U (en) | 2014-12-19 | 2014-12-19 | Mite specific IgG4 subclass antibodies detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420815685.0U CN204359795U (en) | 2014-12-19 | 2014-12-19 | Mite specific IgG4 subclass antibodies detection kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204359795U true CN204359795U (en) | 2015-05-27 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420815685.0U Expired - Fee Related CN204359795U (en) | 2014-12-19 | 2014-12-19 | Mite specific IgG4 subclass antibodies detection kit |
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| Country | Link |
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| CN (1) | CN204359795U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108646023A (en) * | 2018-05-03 | 2018-10-12 | 辽宁汇普源生物医学科技开发有限责任公司 | The ELISA kit of anaphylactogen is passed based on IgG4 antibody test gas |
| CN114441610A (en) * | 2021-12-31 | 2022-05-06 | 江苏省人民医院（南京医科大学第一附属医院） | Electrochemical luminescence detection kit for detecting each subtype of insulin antibody |
-
2014
- 2014-12-19 CN CN201420815685.0U patent/CN204359795U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108646023A (en) * | 2018-05-03 | 2018-10-12 | 辽宁汇普源生物医学科技开发有限责任公司 | The ELISA kit of anaphylactogen is passed based on IgG4 antibody test gas |
| CN114441610A (en) * | 2021-12-31 | 2022-05-06 | 江苏省人民医院（南京医科大学第一附属医院） | Electrochemical luminescence detection kit for detecting each subtype of insulin antibody |
| CN114441610B (en) * | 2021-12-31 | 2023-02-24 | 江苏省人民医院（南京医科大学第一附属医院） | Electrochemical luminescence detection kit for detecting each subtype of insulin antibody |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: WUHAN ZHONGMIN TAIKE BIO-TECHNOLOGY CO., LTD. Free format text: FORMER OWNER: CHEN JIANJUN Effective date: 20150813 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20150813 Address after: 430079 East Lake hi tech Zone, Wuhan hi tech Zone, Hubei, Optics Valley biological City Biotechnology Research Institute, B8 Patentee after: WUHAN ZHONGMIN TAIKE BIOLOGICAL TECHNOLOGY CO.,LTD. Address before: 430079 East Lake high tech Zone, Wuhan hi tech Zone, Hubei, Optics Valley Biological Technology Research Institute, B8 building Patentee before: Chen Jianjun |
|
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150527 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |