CN104391124B - A kind of myoglobins detects the preparation method of reagent - Google Patents
A kind of myoglobins detects the preparation method of reagent Download PDFInfo
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- CN104391124B CN104391124B CN201410648653.0A CN201410648653A CN104391124B CN 104391124 B CN104391124 B CN 104391124B CN 201410648653 A CN201410648653 A CN 201410648653A CN 104391124 B CN104391124 B CN 104391124B
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- reagent
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
- G01N33/721—Haemoglobin
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
Abstract
The present invention relates to a kind of myoglobins and detect reagent, it is formed by reagent one and reagent two cooperation, and wherein, the volume ratio of described reagent one and reagent two is 5: 1; Described reagent one comprises: the damping fluid of 20 ~ 50mmol/L, the set accelerator of 2%w/v, the BSA of 2%w/v, the antiseptic of 0.1% ~ 0.5%w/v; Described reagent two comprises: 0.1% ~ 0.5%w/v is marked with colloid gold particle, the stabilizing agent of 2% ~ 5%w/v, the damping fluid of 20 ~ 50mmol/L, the antiseptic of 0.1% ~ 0.5%w/v of anti-human MYO antibody; The described diameter being marked with the colloid gold particle of anti-human MYO antibody is 60nm ~ 90nm.It is high that the present invention has sensitivity for analysis, detects the range of linearity large, easy to use and detect the plurality of advantages such as quick.
Description
[technical field]
The present invention relates to a kind of myoglobins and detect reagent and preparation method thereof, be specifically related to a kind of for homogeneous phase sol particle type MYO immunoassay reagent detecting MYO in serum or blood plasma and preparation method thereof, belong to medicine equipment external diagnosis reagent field.
[background technology]
Myoglobins (Myoglobin, MYO) be a kind of oxygen in conjunction with hemoprotein, be mainly distributed in cardiac muscle and skeletal muscle tissue.Nineteen sixty is illustrated by Kendrew X-ray diffraction method, and this is first tertiary protein structure be described in the world.When acute myocardial injury, MYO is released in blood at first, and after symptom occurs about 2 ~ 3 hours, in blood, MYO can exceed upper limits of normal, within 9 ~ 12 hours, reaches peak value, recovers normal after 24 ~ 36 hours.For suspecting that the patient of ACS advises that continuous sampling measures, because symptom appearance and protein marker are discharged between blood one section of delay.Have lot of documents report in the clinical efficacy of ACS early diagnosis and monitoring, MYO feminine gender contributes to getting rid of heart stalk.
Mensuration serum myoglobin myoglobins can be used as the sensitiveest early stage index that acute myocardial infarction AMI (AMI) is diagnosed.But poor specificity, the diseases such as Skeletal muscle injury, wound, renal failure, all can cause it to raise.Though the MYO positive can not make a definite diagnosis AMI, can be used for the important indicator getting rid of AMI diagnosis in early days, as MYO is negative, then substantially gets rid of myocardial infarction, also can be used for again the diagnosis of infarct, in conjunction with clinical, as MYO raises again, infarct or infarct extension should be thought of as again.
[summary of the invention]
For solving the problem, the object of the present invention is to provide a kind of sensitivity for analysis high, detecting the range of linearity large, easy to use and detect and detect reagent based on the myoglobins of the even phase immunization of collaurum fast.
The second object of the present invention is to provide a kind of myoglobins to detect the preparation method of reagent.
For realizing above-mentioned first object, the technical scheme that the present invention takes is: a kind of myoglobins detects reagent, and it is formed by reagent one and reagent two cooperation, and wherein, the volume ratio of described reagent one and reagent two is 5: 1; Described reagent one comprises: the damping fluid of 20 ~ 50mmol/L, the set accelerator of 2%w/v, the BSA of 2%w/v, the antiseptic of 0.1% ~ 0.5%w/v; Described reagent two comprises: 0.1% ~ 0.5%w/v is marked with colloid gold particle, the stabilizing agent of 2% ~ 5%w/v, the damping fluid of 20 ~ 50mmol/L, the antiseptic of 0.1% ~ 0.5%w/v of anti-human MYO antibody; The described diameter being marked with the colloid gold particle of anti-human MYO antibody is 60nm ~ 90nm.
Myoglobins of the present invention detects reagent: the damping fluid in described reagent one and reagent two is specially one or more in Tris-HCl damping fluid, HEPES damping fluid, borate buffer solution or the glycine buffer containing 0.5% ~ 5.0%w/vBSA.
Myoglobins of the present invention detects reagent: the pH value of described reagent one, reagent two is 7.0 ~ 9.0.
Myoglobins of the present invention detects reagent: described BSA concentration is 1%w/v, and the pH value of reagent one, reagent two is 8.0 ± 0.2.
Myoglobins of the present invention detects reagent: the set accelerator in described reagent one be in PEG6000 or Brij-35 at least any one.
Myoglobins of the present invention detects reagent: the colloid gold particle diameter in described reagent two is 75nm ~ 85nm, and granule content is 0.08 ~ 0.8mg/mL.
Myoglobins of the present invention detects reagent: described colloid gold particle content is 0.4mg/mL.
Myoglobins of the present invention detect reagent also for: described in be marked with anti-human MYO antibody be the potpourri with the multiple clone strain monoclonal antibody of different immunocompetence site.
For realizing above-mentioned second object, the technical scheme that the present invention takes is: a kind of myoglobins detects the preparation method of reagent, and it comprises the steps:
1), set accelerator, BSA and antiseptic are joined damping fluid, obtained reagent one;
2), gold chloride and trisodium citrate are heated according to mass ratio 1: 1 boil obtained colloidal gold solution; MYO antibody is joined in colloidal gold solution, and adds stabilizing agent, damping fluid, antiseptic, obtained reagent two; Wherein, when configuration gold chloride and citric acid three sodium solution, 0.2 μm of membrane filtration is used;
3), by reagent one and reagent two according to 5: 1 volume ratio with the use of, obtain myoglobins and detect reagent.
For realizing above-mentioned second object, another technical scheme that the present invention takes is: a kind of myoglobins detects the preparation method of reagent, and it comprises the steps:
(1), the preparation of colloid gold particle
1) glass container is first clean with running water with abluent washing, then use silication reagent soaked overnight, silicidation is done to the inside surface of gold vessel ware processed used, cleaner with distilled water flushing, for subsequent use;
2) prepare 1% (w/v) gold chloride and 1% (w/v) citric acid three sodium solution, use 0.2 μm of membrane filtration;
3) in the 1000ml round-bottomed flask cleaned up, put into magnetic stir bar 1 piece, add 1000ml ultrapure water;
4) adding concentration is 1% (w/v) gold chloride, 600rpm high-speed stirred, and is heated to solution boiling, then be that 1% sodium citrate solution joins in flask fast by concentration, keep heating and stir 10min, solution colour first turns black, and then purpling is red gradually;
5) cool: close heater switch and continue to stir 10min, then stopping stirring being cooled to room temperature, return to original volume with ultrapure water;
(2), the preparation of reagent one
By Tris-HCl damping fluid, 0.9%NaCl (w/v), 2%BSA (w/v), 0.1%NaN3 (w/v), 0.05%Tween20 (w/v) mixing, and the concentration of quality control damping fluid according to Tris, the pH value of damping fluid is adjusted according to the use amount of HCl;
(3) preparation of the colloid gold particle of anti-human MYO antibody, is marked with
Getting 1 milliliter of collaurum liquid prepared joins in the centrifuge tube of 1.5 milliliters;
Colloidal gold solution in centrifuge tube is adjusted between pH9 ~ 10 by the solution of potassium carbonate with 10%;
Antibody is added in above-mentioned centrifuge tube, shakes 30 minutes;
Whether in above-mentioned solution, add the NaCl solution of 100uL10%, mixing, observing each pipe after leaving standstill 2h has color change or Precipitation, when showing that the antibody molecule added is less than albumen a maximum demand without color change with without Precipitation;
500ml colloidal gold solution is added in triangular flask, while stirring with 10% pH value to 9 ~ 10 of sal tartari adjustment solution, and by pH detection paper adjustment process;
Join in above-mentioned solution by the antibody consumption determined, continue stirring 30 minutes, label is collected in ultrafiltration;
(4), the preparation of reagent two
By Tris-HCl damping fluid, 0.9% (w/v) NaCl, 2% (w/v) BSA, 0.1% (w/v) NaN3,0.05% (w/v) Tween20,5% (w/v) sucrose or trehalose, 2% (w/v) glycocoll, the label of 2% (w/v) PEG6000 and step (three) mixes obtained reagent two mutually;
(5), by reagent one and reagent two according to 5: 1 volume ratio with the use of, obtain myoglobins and detect reagent.
Compared with prior art, the present invention has following beneficial effect:
1. the present invention utilizes the even phase particle characteristics of collaurum, by specific MYO antibody labeling in colloid gold particle surface, when there is MYO in detection system or testing environment, the antibody on colloid gold particle surface is about to antigen capture corresponding with it, and form antigen-antibody complex, and then cause polymerization or the accumulation of local colloid gold particle, the even phase reagent transmittance spectrum of collaurum is moved to blue color spectrum by redness, this mobile key reaction is the reduction of 540nm place absorbance and the rising of 660nm place absorbance, thus reach the object quantitatively detecting MYO antigen in a corpse or other object for laboratory examination and chemical testing, avoid the shortcoming that similar test item Immunoturbidimetry reagent produces latex microsphere cross-linking agent absorption cuvette not easy cleaning after the reaction simultaneously.
2. the present invention can be used for detecting MYO content in serum or blood plasma, is applicable to the instruments such as spectrophotometer, semi-automatic biochemical analyzer and the automatic clinical chemistry analyzer used in clinical detection process.
3. the sensitivity that the present invention detects MYO can reach 5ng/mL, detects the range of linearity and reaches 5 ~ 300ng/mL, have high analyte sensitivity, the features such as high specific.
[accompanying drawing explanation]
Fig. 1 is the colloid gold label thing calibration graph for variable grain size.
Fig. 2 is reagent of the present invention and the linear comparison diagram of commercially available related reagent analysis.
[embodiment]
First, it should be noted that, the w/v related in the present invention is designated as " w/v ", and unit is " g/mL ".
The present invention is that a kind of myoglobins detects reagent, and it is mixed by reagent I and reagent II, and wherein, the volume ratio of described reagent I and reagent II is 5: 1.
Described reagent I comprises: the damping fluid of 20 ~ 50mmol/L, the set accelerator of 2%w/v, the BSA of 2%w/v, the antiseptic of 0.1% ~ 0.5%w/v.Wherein, one or more during the damping fluid of described reagent I is specially containing 0.5% ~ 5.0%w/vBSA Tris-HCl damping fluid, HEPES damping fluid, borate buffer solution or glycine buffer.The pH value of described reagent I is 7.0 ~ 9.0, and preferred pH value is 8.0 ± 0.2.Described BSA concentration is 1%w/v.Described set accelerator be in PEG6000 or Brij-35 at least any one.
Described reagent II comprises: 0.1% ~ 0.5%w/v is marked with colloid gold particle, the stabilizing agent of 2% ~ 5%w/v, the damping fluid of 20 ~ 50mmol/L, the antiseptic of 0.1% ~ 0.5%w/v of anti-human MYO antibody.Wherein, the diameter being marked with the colloid gold particle of anti-human MYO antibody described in is 60nm ~ 90nm.The damping fluid of described reagent II is specially one or more in Tris-HCl damping fluid, HEPES damping fluid, borate buffer solution or the glycine buffer containing 0.5% ~ 5.0%w/vBSA.The pH value of described reagent II is 7.0 ~ 9.0, and preferred pH value is 8.0 ± 0.2.Preferential colloid gold particle diameter is 75nm ~ 85nm, and particle is unsuitable too small, otherwise reaction velocity can be caused slack-off; Particle is also unsuitable excessive, and label can be caused to assemble sedimentation affects measurement result further.Described colloid gold particle granule content is 0.08 ~ 0.8mg/mL, and preferential granule content is 0.4mg/mL.The described potpourris that to be marked with anti-human MYO antibody be many anti-or have the multiple clone strain monoclonal antibody of different immunocompetence site.
The preparation method that described myoglobins detects reagent is as follows:
1), set accelerator, BSA and antiseptic are joined damping fluid, obtained reagent I;
2), gold chloride and trisodium citrate are heated according to mass ratio 1: 1 boil obtained colloidal gold solution; MYO antibody is joined in colloidal gold solution, and adds stabilizing agent, damping fluid, antiseptic, obtained reagent II; Wherein, when configuration gold chloride and citric acid three sodium solution, 0.2 μm of membrane filtration is used;
3), by reagent I and reagent II according to 5: 1 volume ratio with the use of, obtain myoglobins detect reagent.
It is below the specific embodiment that myoglobins of the present invention detects reagent.
Embodiment one
The preparation (following all operations need complete in the dustless space of high-cleanness, high) of colloid gold particle
1) preliminary work: all glass containers used are first clean with running water with abluent washing, then use silication reagent soaked overnight, do silicidation to the inside surface of gold vessel ware processed used, cleaner with distilled water flushing, for subsequent use;
2) prepare 1% (w/v) gold chloride and 1% (w/v) sodium citrate solution, use 0.2 μm of membrane filtration;
3) in the 1000ml round-bottomed flask cleaned up, put into magnetic stir bar 1 piece, add 1000ml ultrapure water;
4) adding certain volume concentration is 1% (w/v) gold chloride, about 600rpm high-speed stirred, and be heated to solution boiling, then rapid is that 1% sodium citrate solution joins in flask fast by certain volume concentration, keep heating and vigorous stirring 10min, solution colour first turns black, and then purpling is red gradually;
5) cool: close heater switch and continue to stir 10min, then stopping stirring being cooled to room temperature, return to original volume with ultrapure water;
6) colloid gold particle of different-grain diameter can control according to the ratio adding gold chloride and sodium citrate, finally detects 10mL colloidal gold solution with electronics particle size analyzer, uses with the particle size results that particle size analyzer display result is gold grain.
Embodiment two
The preparation of reagent one
The formula of reagent one is as follows: Tris-HCl damping fluid, 0.9%NaCl (w/v), 2%BSA (w/v), 0.1%NaN3 (w/v), 0.05%Tween20 (w/v), according to the concentration of the quality control damping fluid of Tris, the pH value of damping fluid can be adjusted according to the use amount of HCl.
Embodiment three
The preparation of label
Getting 1 milliliter of collaurum liquid prepared joins in the centrifuge tube of 1.5 milliliters;
Colloidal gold solution in centrifuge tube is adjusted between pH8.0 ~ 10.0 by the solution of potassium carbonate with 10%;
By a certain amount of antibody in above-mentioned centrifuge tube, shake 30 minutes;
Whether in above-mentioned solution, add the NaCl solution of 100uL10%, mixing, observing each pipe after leaving standstill 2h has color change or Precipitation, when showing that the antibody molecule added is less than albumen a maximum demand without color change with without Precipitation;
500ml colloidal gold solution is added in triangular flask, while stirring with 10% pH value to 8.0 ~ 10.0 of sal tartari adjustment solution, and detect adjustment process with accurate pH test paper;
Join in above-mentioned solution by described method by the antibody consumption determined, continue stirring 30 minutes, label is collected in ultrafiltration.
Embodiment four
The preparation of reagent two
The biomacromolecule of dissolving in reagent two damping fluid and chemical substance are Tris-HCl damping fluid, 0.9% (w/v) NaCl, 2% (w/v) BSA, 0.1% (w/v) NaN3,0.05% (w/v) Tween20,5% (w/v) sucrose or trehalose, 2% (w/v) glycocoll, 2% (w/v) PEG6000, this part buffer is consistent with reagent one, does not repeat them here.
Reagent two buffer solution prepared is collected with ultrafiltration the label obtained mix mutually, final control mark thing gold grain concentration is 0.4mg/mL.
Embodiment five
The preparation of calibration object
By commercially available people source MYO albumen sterling 2% (w/v) BSA solubilize or dilution, obtained 1200ng/mL calibration object, dilutes according to required concentration gradient 0.9% (w/v) NaCl during use.
Embodiment six
The selection of variable grain degree colloid gold particle
Complete the obtained reagent two of mark according to the colloid gold particle preparing different-grain diameter of colloid gold particle in above embodiment, select same buffer concentration and pH to test.
Experimental technique:
Be successively to mix calibration object, reagent one and reagent two at 3: 250: 50 according to volume ratio, fully react at 37 DEG C.
7180 automatic clinical chemistry analyzers record 540 ± 10nm place absorbance.
Relatively variable grain size colloid gold label thing calibration curve finally determines best grain size.
Judge to select the gold grain of 75nm ~ 85nm scope according to calibration curve (accompanying drawing 1) comparatively suitable, but bulky grain sensitivity for analysis better easily produces HOOK effect to be unfavorable for detecting, and granule is obviously not so good as 75nm ~ 85nm particle in the analysis ability of Spring layer.
Reagent of the present invention and commercially available relevant item reagent analysis linear ratio are comparatively
Test method: for a clinical high level sample, by its doubling dilution as Gradient, detects with contrast agents and reagent of the present invention respectively, and matching it is linear, result is as shown in Figure 2.
Table 1 linear data result
The linear y=0.9733x-0.3368r=0.9999 of reagent of the present invention
The linear y=1.343x-78528r=0.9980 of contrast method reagent
As can be seen from related data statistics, the linear result of reagent of the present invention is obviously better than the linear test result of contrast agents.
Embodiment seven
(4) interference test
A certain amount of cholerythrin, bovine hemoglobin, fat emulsion and rheumatoid factor is added separately in normal human serum, first measure sample initial value, interfering material is added in the sample afterwards according to following table interfering material concentration, only add a kind of chaff interference at every turn, it is made to reach the concentration shown in following table, simultaneously according to the result after measurement result and volume change inverse extension rate computation and measurement, the results are shown in Table 2.
The anti-interference testing result of table 2
Show according to above data, reagent of the present invention meets clinical detection requirement in this chaff interference existence range, illustrates that reagent of the present invention has good interference free performance.
Above embodiment is only the preferred embodiment of this creation, and not in order to limit this creation, any amendment made within all spirit in this creation and principle, equivalent replacement, improvement etc., within the protection domain that all should be included in this creation.
Claims (1)
1. myoglobins detects a preparation method for reagent, it is characterized in that: comprise the steps:
(1), the preparation of colloid gold particle
1) glass container is first clean with running water with abluent washing, then use silication reagent soaked overnight, silicidation is done to the inside surface of gold vessel ware processed used, cleaner with distilled water flushing, for subsequent use;
2) prepare 1% (w/v) gold chloride and 1% (w/v) citric acid three sodium solution, use 0.2 μm of membrane filtration;
3) in the 1000ml round-bottomed flask cleaned up, put into magnetic stir bar 1 piece, add 1000ml ultrapure water;
4) adding concentration is 1% (w/v) gold chloride, 600rpm high-speed stirred, and is heated to solution boiling, then be that 1% sodium citrate solution joins in flask fast by concentration, keep heating and stir 10min, solution colour first turns black, and then purpling is red gradually;
5) cool: close heater switch and continue to stir 10min, then stopping stirring being cooled to room temperature, return to original volume with ultrapure water;
(2), the preparation of reagent one
By Tris-HCl damping fluid, 0.9%NaCl (w/v), 2%BSA (w/v), 0.1%NaN3 (w/v), 0.05%Tween20 (w/v) mixing, and the concentration of quality control damping fluid according to Tris, the pH value of damping fluid is adjusted according to the use amount of HCl;
(3) preparation of the colloid gold particle of anti-human MYO antibody, is marked with
Getting 1 milliliter of collaurum liquid prepared joins in the centrifuge tube of 1.5 milliliters;
Colloidal gold solution in centrifuge tube is adjusted between pH9 ~ 10 by the solution of potassium carbonate with 10%;
Antibody is added in above-mentioned centrifuge tube, shakes 30 minutes;
Whether in above-mentioned solution, add the NaCl solution of 100uL10%, mixing, observing each pipe after leaving standstill 2h has color change or Precipitation, when showing that the antibody molecule added is less than albumen a maximum demand without color change with without Precipitation;
500ml colloidal gold solution is added in triangular flask, while stirring with 10% pH value to 9 ~ 10 of sal tartari adjustment solution, and by pH detection paper adjustment process;
Join in above-mentioned solution by the antibody consumption determined, continue stirring 30 minutes, label is collected in ultrafiltration;
(4), the preparation of reagent two
By Tris-HCl damping fluid, 0.9% (w/v) NaCl, 2% (w/v) BSA, 0.1% (w/v) NaN3,0.05% (w/v) Tween20,5% (w/v) sucrose or trehalose, 2% (w/v) glycocoll, the label of 2% (w/v) PEG6000 and step (three) mixes obtained reagent two mutually;
(5), by reagent one and reagent two according to 5: 1 volume ratio with the use of, obtain myoglobins and detect reagent.
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| CN104330575B (en) * | 2014-11-17 | 2017-10-03 | 中国人民解放军第三0五医院 | A kind of Troponin I detection reagent and preparation method thereof |
| CN106950363A (en) * | 2017-03-31 | 2017-07-14 | 四川迈克生物科技股份有限公司 | Suppress the latex enhancing immune of rheumatoid factor interference than turbid reagent |
| CN111665355A (en) * | 2020-05-06 | 2020-09-15 | 量准（上海）医疗器械有限公司 | Kit based on nano plasma resonance molecules and testing method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6068986A (en) * | 1994-01-13 | 2000-05-30 | The Board Of Governors For Higher Education | Antibodies specific for d-myo-inositol 1,4,5-trisphosphate and the enzyme-linked immunosorbent assay of d-myo-inositol 1,4,5-trisphosphate |
| JP2005283250A (en) * | 2004-03-29 | 2005-10-13 | Alfresa Pharma Corp | Measuring method of gold colloid agglutination reaction |
| JP2009057291A (en) * | 2007-08-29 | 2009-03-19 | Marudai Food Co Ltd | Antibody to bovine myoglobin partial peptide and method for testing using the antibody and kit for testing |
| CN101699287A (en) * | 2009-09-08 | 2010-04-28 | 北京利德曼生化股份有限公司 | Homogeneous phase sol particle type cystatin C measuring kit and preparation method thereof |
| CN102749454A (en) * | 2012-06-11 | 2012-10-24 | 宁波鼎鑫生物科技有限公司 | Full-scale C-reactive protein (CRP) colloidal gold immunoturbidimetric assay kit |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102323422B (en) * | 2011-05-30 | 2014-03-12 | 中国科学院上海微系统与信息技术研究所 | Immunochromatographic test strip for semi-quantitatively and simultaneously detecting cTnI and Myo and preparation method thereof |
-
2014
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6068986A (en) * | 1994-01-13 | 2000-05-30 | The Board Of Governors For Higher Education | Antibodies specific for d-myo-inositol 1,4,5-trisphosphate and the enzyme-linked immunosorbent assay of d-myo-inositol 1,4,5-trisphosphate |
| JP2005283250A (en) * | 2004-03-29 | 2005-10-13 | Alfresa Pharma Corp | Measuring method of gold colloid agglutination reaction |
| JP2009057291A (en) * | 2007-08-29 | 2009-03-19 | Marudai Food Co Ltd | Antibody to bovine myoglobin partial peptide and method for testing using the antibody and kit for testing |
| CN101699287A (en) * | 2009-09-08 | 2010-04-28 | 北京利德曼生化股份有限公司 | Homogeneous phase sol particle type cystatin C measuring kit and preparation method thereof |
| CN102749454A (en) * | 2012-06-11 | 2012-10-24 | 宁波鼎鑫生物科技有限公司 | Full-scale C-reactive protein (CRP) colloidal gold immunoturbidimetric assay kit |
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