CN108918866A - A kind of marker of inflammation POCT combined detection kit suit - Google Patents
A kind of marker of inflammation POCT combined detection kit suit Download PDFInfo
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- G—PHYSICS
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
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- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
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- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
- G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
- G01N2333/4701—Details
- G01N2333/4737—C-reactive protein
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Abstract
The invention discloses a kind of marker of inflammation POCT combined detection kit suit, including detection card, colorimetric card, sample collection device, detection device, detection card is got stuck by test strips and rectangle to be formed；Test strips are made of bottom plate, sample pad, bonding pad, nitrocellulose filter and water absorption pad, and sample pad, bonding pad, nitrocellulose filter and water absorption pad are successively overlapped and be assembled on bottom plate, CRP and SAA antibody-fluorescent microsphere compound is fixed on bonding pad；It is coated with anti-SAA monoclonal antibody, anti crp monoclonal antibody on nitrocellulose filter in detection zone, forms T1, T2, T3, T4 detection line.The present invention provides a kind of early stage portable, small in size, easy to operate, easy to use and marker of inflammation POCT joint-detection SAA, the CRP kit suits of antidiastole.
Description
Technical field
The invention belongs to immuno-chromatographic assay technology fields, and in particular to a kind of marker of inflammation POCT of portable domestic
Combined detection kit suit.
Background technique
Infectious diseases refers to various pathogenic microorganism invasion human bodies, leads to the disease of body inflammatory or organ dysfunction,
Human health is seriously threatened, quality of life is influenced.It is early diagnosed, and Using adapted Antibios are diffused with very to control infection
Important role.C reactive protein（CRP）It is a kind of Acute reaction protein synthesized by liver, in most virus infections
In reduced levels；And when bacterium infection or damage, it can increase even hundreds times or more of several times, decades of times, elevation amplitude and thin
The degree of bacterium infection is positively correlated, and after rational therapy, about 3~7d can drop to normal level, feels to discriminating bacteria and virus
It is infected with very important effect.Serum amyloid protein（SAA）It is the precursor substance of tissue amyloid A, under normal circumstances
Its content is less and more stable, body virus and（Or）After the invasion of bacterial disease opportunistic pathogen, peak value, amplification are reached in 4~6 h
Greatly, the convalescence of disease decline rapidly again.SAA can be used as the marker of sensitive infectious diseases for clinic diagnosis, become
Another New Set of early stage auxiliary diagnosis after white blood cell count(WBC) and CRP, joint-detection SAA, CRP feel bacterium and virus
Contaminating antidiastole has biggish clinical meaning.
The Inflammatory Mediators such as Hospitals at Present detection CRP, SAA mainly use large-scale biochemical instruments, and advantage mainly detects knot
The disadvantages of fruit is accurate, but that there are detection times is long, and required specimen amount is more, complicated for operation, higher to operator's level requirement, and
Detecting instrument is expensive, and only part large hospital could be equipped with, and is restricted its application.In recent years, POCT detects skill
Art is quickly grown, and becomes a Novel test technology.Wherein fluorescence immune chromatography technology is being clinical with its unique performance
Laboratory is received, and is compared with the traditional method, it has, and easy to operate, speed is fast, pollute less, result is stablized accurately；It can adopt
With minimally invasive tip（Finger, ear-lobe）Blood sampling reduces blood sampling volume；It is also possible to shorten patient's hospital stays, it is all to save hospitalization cost etc.
More advantages.
There are the fluorescence immune chromatography kit patent of joint-detection CRP and SAA at present（Application number：
201521063805.7）If but simultaneous quantitative detects CRP and SAA, needs matched fluorescence immunity analyzer, a fluorescence
Immunity analysis instrument also needs tens of thousands of members, limits it in the application of family.And kit detection suit cost provided by the invention
Cheap, volume is light and handy, is not required to mating special fluorescence immune chromatography instrument, easy to use.
Summary of the invention
The present invention provides a kind of portable, small in size, easy to operate, easy to use infectious diseases early diagnosis and
Marker of inflammation POCT joint-detection SAA, the CRP kit of antidiastole is set with.
The technical solution adopted in the present invention is as follows：
This kit suit, including detection card, colorimetric card, sample collection device, detection device, the detection block by test strips with
Rectangle gets stuck composition；The test strips are made of bottom plate, sample pad, bonding pad, nitrocellulose filter and water absorption pad, the sample
Product pad, bonding pad, nitrocellulose filter and water absorption pad are successively overlapped and are assembled on bottom plate, wherein water absorption pad and bonding pad difference
The overlapping both ends for being pressed in nitrocellulose filter, and detection zone is formed on the surface of nitrocellulose filter；Sample pad is overlapping to be pressed in knot
It closes on pad, CRP and SAA antibody-fluorescent microsphere compound is fixed on the bonding pad；Nitrocellulose in the detection zone
It is coated with two anti-SAA monoclonal antibodies, two anti crp monoclonal antibodies on film, is successively used as T1, T2, T3, T4 detection line,
In, Eu- carboxyl fluorescent microsphere is activated by EDC-NHS two-step method, forms compound with CRP and SAA antibody coupling, then through multiple
Solution redissolves, confining liquid is closed, finally obtained CRP and SAA antibody-fluorescent microsphere compound.
Preferably, two kinds of concentration of the anti-SAA monoclonal antibody and anti crp monoclonal antibody are：0.5mg/ml and
2mg/ml；The group for redissolving liquid becomes 8.0 Tris-HCl of 0.05M PH, 0.1%PVP K30,0.5%BSA, 3% seaweed
Sugar, 1%Triton X-100,0.05%NaN3；The confining liquid is 20%BSA.
Preferably, the preparation method of the detection card, includes the following steps：
（1）Prepare CRP and SAA antibody-fluorescent microsphere compound；
（2）Monoclonal antibody is coated on nitrocellulose filter：Nitrocellulose filter is pasted onto the middle position of bottom plate, from nitric acid
Among cellulose membrane close to the side of bonding pad rise with 1 μ l/cm speed be successively coated with two kinds of concentration anti-SAA monoclonal antibody,
The anti crp monoclonal antibody of two kinds of concentration forms two SAA detection lines, i.e. T1 and T2, two CRP detection lines, i.e. T3 and T4,
Drying；
（3）The preparation of bonding pad：After glass fibre component is soaked in treatment fluid, drying will be walked using three-dimensional point film gold spraying instrument
Suddenly（1）For CRP the and SAA antibody-fluorescent microsphere compound even application of preparation on above-mentioned glass fibre component, prepared by drying
At bonding pad；
（4）Detect the preparation of card：Sample pad, bonding pad, nitrocellulose filter and water absorption pad are successively overlapped and are assembled on bottom plate
The big plate of test paper is obtained, the strip of wide 3.9mm is cut into, test strips is made；The test strips prepared are fitted into during rectangle gets stuck
Clamping is put into the aluminium foil bag added with desiccant, is prepared into the detection card of joint-detection SAA, CRP.
Preferably, step（2）Described in drying condition be：The dry 2h at 37 DEG C.
Preferably, step（3）Described in treatment fluid group become 0.02M Tris-HCL, 0.05%BSA, 0.1%Triton
X-100,3% trehalose；The drying time is 2h；The drying condition is：37 DEG C of temperature, humidity 30%.
Preferably, step（4）Described in rectangle get stuck including plastics upper casing and plastics lower casing, nitre is corresponded on epivalve
The position of sour tunica fibrosa is equipped with observation window, and the position that sample pad is corresponded on epivalve is equipped with well, and well is located at sample
Pad is intermediate.
Preferably, the sample pad is directly affixed on the bottom plate below bonding pad using blood filter membrane, and finger tip can be used directly
Blood or new blood are detected, and keep product more convenient simple and direct when in use, save detection time, improve detection efficiency.
Preferably, the sample collection device is diluted by disposable blood taking needle, chlorination equipment, sample collection bottle and sample
Liquid composition；The sample diluting liquid is by deionized water, 0.05M PBS buffer solution, 0.1%T-20,0.1%BSA, 0.05%NaN3System
At；The chlorination equipment is Iodophor and cotton swab.
Preferably, the detection device includes rectangular detection box, and the top of rectangular detection box is equipped with light source, is set on the outside of light source
There is beam condensing unit, detect position among the side of box and be equipped with reading window, the bottom for detecting box is equipped with detection card reaction zone；The light
Source is ultraviolet lamp, can be irradiated in detection and block on the nitrocellulose filter of interior test strips, the Eu element on fluorescent microsphere is by ultraviolet
Fluorescence is issued after light irradiation, and the content of fluorescence intensity and fluorescent microsphere is positively correlated, and open detection device carries out after being loaded 5min
As a result interpretation realizes the joint-detection to CRP, SAA, early diagnoses in conjunction with result interpretation method provided by the invention to infection
And antidiastole provides foundation, while kit of the present invention is set with the interference that can avoid background fluorescence signal, improves the spirit of detection
Sensitivity and specificity.
Preferably, the invention also includes the operation instructions of marker of inflammation POCT combined detection kit suit.
Application range and advantage of the invention：
The kit suit that the present invention is formed can be applied to community's basic hospital, community health, kindergarten, school, factories and miness, community
Branch Clinic or private doctor clinic, battlefield first aid or used on ambulance and including family, detect easy to operate, institute
Kit suit is obtained to testing staff without particular/special requirement, amateur docimaster, Non-medical specialty personnel, even detected object
I, can carry out operating timely detection to specifications；Can accomplish detection by bed, to inspection result make correctly judgement and
It explains, is conducive to understand the state of an illness in time and takes effective remedy measures；The present invention be minimally invasive detection, i.e., using tip (finger,
Ear-lobe) blood sampling, it is only necessary to super quick CRP, SAA detection can be completed in 1 drop whole blood sample, reduce patient because of pain caused by blood sampling and
The risk of cross-infection.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of test strips；
In figure, 1- sample pad；2- bonding pad；3- nitrocellulose filter；4-T1 line；5-T2 line；6-T3 line；7-T4 line；8- water suction
Pad；9- bottom plate.
Fig. 2 is the structural schematic diagram of detection card；
In figure, 10- rectangle gets stuck；11-T2 line；12-T3 line；13-T4 line；14- well；15-T1 line；16- observation window.
Fig. 3 is the structural schematic diagram of detection device；
In figure, 17- ultraviolet lamp；18- beam condensing unit；19- reads window；20- detects card reaction zone；The rectangular detection box of 21-.
Fig. 4 is the structural schematic diagram of colorimetric card.
Specific embodiment
Below by specific embodiment, the invention will be further described.
The preparation of the detection card of embodiment 1
1. prepared by CRP and SAA antibody-fluorescent microsphere compound
It takes 10 μ L Eu- carboxyl fluorescent microspheres that the borate buffer of 400 PH=7.6 μ L 0.05M is added, mixes；40 μ L are added
The EDC activator of 1mg/ml and 10 μ L 1mg/ml NHS solution, in oscillation activation 20min on constant temperature oscillator；Then in
It is centrifuged 20min under 5000rpm, abandons supernatant, the 500 μ L 0.05M borate buffers of pH=7.6 are added and redissolve precipitating, whirlpool mixes,
It is separately added into 40ug CRP, SAA, whirlpool mixes, and is put into shaking table low-speed oscillation coupling 2h；It is centrifuged 15 min under 5000rpm, abandons
Supernatant is added 1500 μ L and redissolves liquid and redissolves, then with 10 μ L confining liquids（20%BSA）30min is closed, the group for redissolving liquid becomes
8.0 Tris-HCl of 0.05M PH, 0.1%PVP K30,0.5%BSA, 3% trehalose, 1%Triton X-100,0.05%
NaN3。
2. being coated with monoclonal antibody on nitrocellulose filter
Anti crp monoclonal antibody, anti-SAA monoclonal antibody are diluted using 0.05M PBS, are diluted to 0.5mg/ml, 2mg/ respectively
Ml is simultaneously marked；Nitrocellulose filter is pasted onto bottom plate（PVC board）Middle position, setting three-dimensional point film gold spraying instrument nitric acid fibre
It ties up and the anti-SAA monoclonal antibody of 2mg/ml, 0.5mg/ is coated with successively with 1 μ l/cm speed from the side of close bonding pad on plain film
The anti-SAA monoclonal antibody of ml, 2mg/ml anti crp monoclonal antibody, 0.5mg/ml anti crp monoclonal antibody, respectively as detection
Line 1（T1）, detection line 2（T2）, detection line 3（T3）, detection line 4（T4）, dry 2h is placed at 37 DEG C.
3. the preparation of bonding pad
It is roomy small that glass fibre component is cut into 30mm*7mm, adds 150 μ L treatment fluids to carry out immersion treatment according to every 4cm, pulls out
It is placed in electric drying oven with forced convection dry 2h, using three-dimensional point film gold spraying instrument by CRP the and SAA antibody-fluorescent microballoon of preparation
Compound adds the ratio uniform of 150 μ L to be sprayed on the glass fibre component handled well, is placed in drying room according to every 4cm, in
The lower dry 2h of 37 DEG C of temperature, humidity 30%, is prepared into bonding pad；The group of the treatment fluid becomes 0.02M Tris-HCL, 0.05%
BSA, 0.1%Triton X-100,3% trehalose.
4. detecting the preparation of card：Sample pad, bonding pad, nitrocellulose filter and water absorption pad are successively overlapped and are assembled in bottom
Plate（PVC board）On, wherein water absorption pad and bonding pad overlap the both ends for being pressed in nitrocellulose filter respectively, and in nitrocellulose
The surface of film forms detection zone；Sample pad is overlapping to be pressed on bonding pad, and the big plate of test paper is obtained after assembling, is cut into wide 3.9mm's
Test strips are made in strip；The test strips prepared are fitted into the rectangle with plastics upper casing and plastics lower casing to get stuck middle clamping,
It is put into the aluminium foil bag added with desiccant, is prepared into the detection card of joint-detection SAA, CRP；Wherein, nitre is corresponded on epivalve
The position of sour tunica fibrosa is equipped with observation window, and the position that sample pad is corresponded on epivalve is equipped with well, and well is located at sample
Pad is intermediate.
6. result judges
After the well sample-adding 5min of detection card, interpretation result will test in the detection card reaction zone for be placed in detection device simultaneously
It is compared with colorimetric card.
If detection card detection zone T1, T2, T3, T4 have fluorescence, and T2, T4 fluorescence intensity and colorimetric card C2 fluorescence intensity phase
When SAA, CRP content are above 20mg/L in expression measurement sample, prompt positive（+）, it is understood that there may be infection.
If detection card detection zone T1, T2, T3, T4 have fluorescence, and T2, T4 fluorescence intensity and colorimetric card C3 fluorescence intensity phase
When SAA content prompts weakly positive in 8 ~ 20mg/L, CRP content in 16 ~ 20mg/L in expression measurement sample（±）, need further
Inspection is clarified a diagnosis.
If detection card detection zone T4, T2 have no that fluorescence, T1, T3 have fluorescence, indicate that SAA content is lower than 4mg/ in measurement sample
L, CRP content is lower than 8mg/L, prompts negative（-）, body is uninfected by.
The preparation of 2 fluorescence immune chromatography colorimetric card of embodiment
1. gradient fluorescent microsphere dilutes
A. take the 10 μ L of microballoon stoste that concentration is 10mg/mL into centrifuge tube.
B. plus 0.05M PBS is diluted to concentration to be followed successively by 12.5 μ g/mL, 6.25 μ g/mL, the microballoon of 3.125 μ g/mL molten
Liquid；
2. scribing line
A. cellulose nitrate film is taken, is pasted onto PVC board, is placed on the corresponding position of three-dimensional point film gold spraying instrument, for use；
B. the two root canal road 3 times of three-dimensional point film gold spraying instrument is cleaned with pure water；
C. conduit positions are adjusted, two root canal road interval 6mm are made；
D. stroked parameters are set as：Cross speed 50mm/s, 1 μ L/cm of scribing line amount；
It e. will be in the microspheres solution of prepared various concentration successively suction line with three-dimensional point film gold spraying instrument；Wherein C1, C2,
C3, C4 line fluorescent microsphere concentration are followed successively by 12.5 μ g/mL, 6.25 μ g/mL, 3.125 μ g/mL, 0 μ g/mL), click start button
It crosses on nitrocellulose filter afterwards, after scribing line, detergent line 3 times, closes instrument.
3. dry
The PVC board prepared is 2 hours dry at 37 DEG C.
4. slitting
Take it is dried after PVC bottom plate, be put into cutting machine, be cut into width be 3.9mm test strips.
It gets stuck 5. adding
The ratio vitta cut is put in inside cartridge card slot, is collected in aluminium foil bag respectively after pressure shell by concentration, is put into desiccant
Sealing saves afterwards.
6. detection
Above-mentioned colorimetric card is successively put in this kit detection system, fluorescence intensity is observed and printing of taking pictures is prepared into colorimetric card
Suit.
Embodiment 3
The detection limit of SAA and CRP antibody on present invention detection card is measured
Sample process：Blood is acquired using the sample collection device that this kit suit provides.
Detect the preparation of card：Detection card prepared by embodiment 1 is placed spare after 15min at room temperature.
By SAA and CRP antigen diluent at 2mg/L, 4mg/L, 8mg/L, 16mg/L, 32mg/L, 64mg/L, 128mg/L,
Joint-detection SAA, the CRP fluorescence immune chromatography kit suit established using the present invention is detected, after being loaded 5min,
It is placed in interpretation result in detection device.As a result such as table 1：
The detection of 1 SAA and CRP antibody of table limits measurement result
As shown in Table 1：The detection card detects serum amyloid A protein（SAA）And c reactive protein（CRP）The series of antigen diluent
Sample is respectively 8mg/L and 16mg/L by the detection limit that testing result knows that detection blocks upper SAA and CRP antibody.
Embodiment 4
The accuracy for joint-detection SAA, the CRP fluorescence immune chromatography kit suit that the present invention establishes is measured
Each 10 parts of SAA and CRP positive sample are taken, 10 parts of normal person's sample, joint-detection SAA, CRP established using the present invention is glimmering
Light immune chromatography reagent kit suit is detected, and after being loaded 5min, is placed in interpretation result in detection device, and observe with colorimetric card
Comparison.As a result such as table 2：
The measurement result of 2 accuracy of table
As shown in Table 2, SAA positive sample and CRP positive sample testing result are the positive, normal person's sample（' negative ' specimens）Inspection
Surveying result is feminine gender.The result is consistent completely with expection, illustrates that test strips accuracy rate provided by the invention is high, meets the requirements.
Embodiment 5
The accuracy for joint-detection SAA, the CRP fluorescence immune chromatography kit suit that the present invention establishes is measured
By SAA, CRP accuracy quality-control product prepared from the well sample-adding of detection card, it is placed in detection device, is examined after 5min
It surveys and reads the fluorescence intensity of T1 line, T2 line, T3 line, T4 line, repeat detection ten times, calculate separately ten testing results, four
Detection line（T1,T2,T3,T4）The coefficient of variation of fluorescence intensity（CV=SD/）, respectively less than 15%, meet accuracy requirement.
6 pattern detection of embodiment
Using 50 parts of the infectious diseases made a definite diagnosis through pathogeny detection and 52 parts of health examination sample, the connection established using the present invention
It closes detection SAA, CRP fluorescence immune chromatography kit suit to be detected, after being loaded 5min, is placed in interpretation knot in detection device
Fruit.The results show that joint-detection SAA, CRP fluorescence immune chromatography quantification kit suit detection bacterium of the invention, virus sense
The sensibility of dye is respectively 94.2%, 91.3%, specificity reach 100%.
Claims (10)
1. a kind of marker of inflammation POCT combined detection kit suit, which is characterized in that including detecting card, colorimetric card, sample
Collection device, detection device, the detection card is got stuck by test strips and rectangle to be formed；The test strips by bottom plate, sample pad,
Bonding pad, nitrocellulose filter and water absorption pad composition, the sample pad, bonding pad, nitrocellulose filter and water absorption pad are successively taken
It connects and is assembled on bottom plate, the anti-SAA monoclonal antibody of two kinds of concentration of coating, two kinds on the nitrocellulose filter in the detection zone
The anti crp monoclonal antibody of concentration is successively used as T1, T2, T3, T4 detection line.
2. marker of inflammation POCT combined detection kit is set with according to claim 1, which is characterized in that the anti-SAA
Two kinds of concentration of monoclonal antibody and anti crp monoclonal antibody are：0.5mg/ml and 2mg/ml；The group for redissolving liquid becomes
8.0 Tris-HCl of 0.05M PH, 0.1%PVP K30,0.5%BSA, 3% trehalose, 1%Triton X-100,0.05%
NaN3；The confining liquid is 20%BSA.
3. marker of inflammation POCT combined detection kit is set with according to claim 1, which is characterized in that the detection card
Preparation method, include the following steps：
（1）Prepare CRP and SAA antibody-fluorescent microsphere compound；
（2）Monoclonal antibody is coated on nitrocellulose filter：Nitrocellulose filter is pasted onto the middle position of bottom plate, and successively
It is coated with the anti-SAA monoclonal antibody and anti crp monoclonal antibody of various concentration, forms two SAA detection lines, i.e. T1 and T2, two
CRP detection line, i.e. T3 and T4, drying；
（3）The preparation of bonding pad：After glass fibre component is soaked in treatment fluid, drying, by step（1）The CRP and SAA of preparation
For antibody-fluorescent microsphere compound even application on above-mentioned glass fibre component, drying is prepared into bonding pad；
（4）Detect the preparation of card：Sample pad, bonding pad, nitrocellulose filter and water absorption pad are successively overlapped and are assembled on bottom plate,
Into strips, test strips are made in cutting；The test strips prepared are fitted into rectangle to get stuck middle clamping, be prepared into joint-detection SAA,
The detection card of CRP.
4. detecting the preparation method of card according to claim 3, which is characterized in that step（2）The drying condition is：In 37
Dry 2h at DEG C.
5. detecting the preparation method of card according to claim 3, which is characterized in that step（3）The group of the treatment fluid becomes
0.02M Tris-HCL, 0.05%BSA, 0.1%Triton X-100,3% trehalose；The drying time is 2h；The dried strip
Part is：37 DEG C of temperature, humidity 30%.
6. detecting the preparation method of card according to claim 3, which is characterized in that step（4）The rectangle get stuck including
Plastics upper casing and plastics lower casing, the position that nitrocellulose membrane is corresponded on epivalve are equipped with observation window, correspond to sample on epivalve
The position of product pad is equipped with well, and well is located among sample pad.
7. marker of inflammation POCT combined detection kit is set with according to claim 1, which is characterized in that the sample pad
It is directly affixed on using blood filter membrane on the bottom plate below bonding pad.
8. marker of inflammation POCT combined detection kit is set with according to claim 1, which is characterized in that the sample is received
Acquisition means are made of disposable blood taking needle, chlorination equipment, sample collection bottle and sample diluting liquid；The sample diluting liquid by go from
Sub- water, 0.05M PBS buffer solution, 0.1%T-20,0.1%BSA, 0.05%NaN3It is made；The chlorination equipment is Iodophor and cotton swab.
9. marker of inflammation POCT combined detection kit is set with according to claim 1, which is characterized in that the colorimetric card
On be successively arranged C1, C2, C3, C4 line；C1, C2, C3, C4 line has drawn fluorescent microsphere solution, and four C lines on colorimetric card
Fluorescence intensity is all different.
10. marker of inflammation POCT combined detection kit is set with according to claim 1, which is characterized in that the detection
Device includes rectangular detection box, and the top of rectangular detection box is equipped with ultraviolet source（Wavelength：300-350nm）, light source outside is equipped with
Beam condensing unit detects position among the side of box and is equipped with reading window, and the bottom for detecting box is equipped with detection card reaction zone.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810644711.0A CN108918866A (en) | 2018-06-21 | 2018-06-21 | A kind of marker of inflammation POCT combined detection kit suit |
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| CN110133281A (en) * | 2019-05-06 | 2019-08-16 | 中生北控生物科技股份有限公司 | CRP and SAA combined detection kit and preparation method thereof |
| CN110927388A (en) * | 2019-11-25 | 2020-03-27 | 益善生物技术股份有限公司 | Preparation method of CRP and SAA combined detection test strip, and prepared test strip, reagent card and kit |
| CN111487230A (en) * | 2020-04-20 | 2020-08-04 | 中国农业科学院烟草研究所 | Ralstonia solanacearum detection device |
| CN112485446A (en) * | 2020-11-18 | 2021-03-12 | 重庆中元汇吉生物技术有限公司 | Kit for measuring full-range C-reactive protein and preparation method thereof |
| CN113552338A (en) * | 2020-04-26 | 2021-10-26 | 格林生物医药科技(天津)有限公司 | Allergen specificity IgE antibody detection kit and preparation method thereof |
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