CN208443851U - Immunofluorescence for detecting cat serum amyloid A protein chromatographs detection card - Google Patents
Immunofluorescence for detecting cat serum amyloid A protein chromatographs detection card Download PDFInfo
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Abstract
The immunofluorescence that the utility model discloses a kind of for detecting cat serum amyloid A protein chromatographs detection card；It is intended to provide a kind of high sensitivity, testing result is reliably used to detect the immunofluorescence chromatography detection card of cat serum amyloid A protein；Technical points include bottom plate, successively linking has sample pad, bonding pad, nitrocellulose membrane and water absorption pad on bottom plate, the bonding pad is equipped with the SAA monoclonal antibody of fluorescent microsphere label and the goat-anti chicken IgY of fluorescent microsphere label, the nitrocellulose membrane is equipped with the detection T line of a coating SAA monoclonal antibody and the Quality Control C line of a coating chicken IgY；Belong to animal epidemic detection field.
Description
Technical field
The utility model discloses a kind of immunofluorescences to chromatograph detection card, specifically, being a kind of for detecting cat serum
The immunofluorescence chromatography detection card of amyloid A.
Background technique
Serum amyloid A protein (SAA) be present in the main acute phase protein of one of many species, including people, dog,
Cat and horse etc..It is infected once body, after wound and surgical operation etc. cause inflammatory stimulus, the concentration of SAA can be in a few hours
Interior rapid raising；SAA half-life period is very short simultaneously, and with the removing in inflammation source, the concentration level of SAA can decline rapidly again.
The gene order of SAA is highly conserved in vertebrate.People SAA contains 104 amino acid.And the SAA of cat is suitable
111 additional amino acid sequences are inserted in the SAA molecule intermediate region in people.
SAA is a kind of sensitive inflammation and tissue damage marker.In veterinary science, the SAA measured in blood can be used
In Diagnosis of subclinical inflammation, the therapeutic effect of monitoring Ii _ i_iLLci _ i_ and infection and monitoring sufferer surgical operation process.
Existing SAA detection method is mainly enzyme-linked immunization, since the enzyme-linked immunosorbent assay period is long, cannot given birth to automatically
Change and used on analyzer, be not suitable for the quick detection of emergency treatment, is the universal drawback of SAA detection kit, and latex enhancing immune
Turbidimetry is easy to operate, economical, can use on the automatic biochemistry analyzer of most hospitals, especially emergency treatment is able to achieve
Rapid quantitative detection, the basic principle is that: antibody is coated on latex particle, after being immunoreacted with corresponding antigens, shape
At aggregated particle, under certain wavelength, by turbidity caused by measurement aggregation, checking matter content in sample can be measured.
A kind of rapid quantitative detection serum amyloid A protein kit of Chinese patent ZL 201210575603.5, the examination
Agent box includes that the anti-SAA immune colloid gold of freeze-drying, reaction plate, sample diluting liquid, freeze-drying made of colloidal gold are coated in SAA monoclonal antibody
Colloidal gold redissolves liquid, cleaning solution and confining liquid；The reaction plate is colloidal gold detection plate, and the nitrocellulose film on reaction plate is by needle
Another purifying SAA monoclonal antibody or how anti-coating to another reaction determinant；But this method uses immunity percolation method, needs to dissolve
Anti- SAA gold standard liquid dried frozen aquatic products simultaneously dilute, diluted sample, confining liquid, infiltration are added dropwise, the anti-SAA gold standard liquid diluted is added dropwise plus washes
Liquid, the finally series of steps such as detection are washed, it is very cumbersome time-consuming, it is unfavorable for disease Rapid&Early diagnosis and treatment.In addition this is special
Benefit is used as tracer using colloidal gold, and immune marker is by Electrostatic Absorption, and that there are sensitivity is lower, it is quantitative, repeated to be difficult to
The defects of poor with stability, can not fully meet clinical detection demand.
Chinese patent ZL201510497125.4 discloses a kind of human serum amyloid A detection kit, described
Kit includes reagent R1, reagent R2, and the reagent R1 is buffer appropriate；The reagent R2 is employment serum amyloid
Sample protein A antibody sensitization polystyrene latex particles are placed in buffer appropriate and form, but this method use it is slow to two kinds
Fliud flushing, liquid need to refrigerate, and when detection needs using automatic biochemistry analyzers such as 7080 types of Hitachi, and volume is relatively large, does not conform to
Suitable on-site test, cannot be to the Clinics and Practices carried out as early as possible.
Chinese patent ZL201511020151.4 discloses a kind of fluorescence immune chromatography detection examination of serum amyloid A protein
Agent box, including reagent box body and dilution bottle, the reagent box body includes immunochromatographydetection detection card, the immunochromatography detection
Card includes that PVC liner plate and the sample pad being fixed on PVC liner plate, probe fix pad, nitrocellulose filter and blotting paper, described
Sample pad is overlapped on the fixed pad of probe, and the fixed pad of the probe and blotting paper are overlapped on the nitrocellulose filter two respectively
Side is provided with detection line and control line on the nitrocellulose filter, and the detection line endoperidium has serum amyloid A protein
Monoclonal antibody, the control line endoperidium have goat anti-rabbit igg；The fixed pad of the probe is by serum amyloid A protein monoclonal
The mixing fluorescent latex particles of antibody fluorescence present latex particulate and goat anti-rabbit igg fluorescent latex particles composition are dry to be made；But it should
There are following disadvantages for method: fluorescent latex particles need first is coupled with albumin, then again with serum amyloid A protein list
Clonal antibody is coupled, although the space resistance of double fastener core structure in detection process can be reduced, while also increasing coupling
The step of, the repeatability of detection may be influenced.
Utility model content
In view of the above problems, the object of the present invention is to provide a kind of high sensitivities, reproducible, quantitative accurately use
In the immunofluorescence chromatography detection card of detection cat serum amyloid A protein.
For this purpose, technical solution provided by the present application is such that
A kind of immunofluorescence chromatography detection card for detecting cat serum amyloid A protein, including shell, the shell
The interior immunofluorescence being equipped with for detecting cat serum amyloid A protein chromatographs detection card, described for detecting cat serum amyloid sample
The immunofluorescence chromatography detection card of albumin A includes bottom plate, and successively linking has sample pad, bonding pad, nitrocellulose membrane on bottom plate
And water absorption pad, the bonding pad are equipped with the SAA monoclonal antibody of fluorescent microsphere label and the goat-anti chicken of fluorescent microsphere label
IgY, the nitrocellulose membrane are equipped with the Quality Control C line of a coating chicken IgY and the inspection of a coating SAA monoclonal antibody
Survey T line.
Further, a kind of above-mentioned immunofluorescence for detecting cat serum amyloid A protein chromatographs detection card, special
Sign is, further includes box body, and the box body is interior equipped with sample dilution bottle and glimmering for detecting being immunized for cat serum amyloid A protein
Photosphere analysis detection card.
Further, a kind of above-mentioned immunofluorescence for detecting cat serum amyloid A protein chromatographs detection card, special
Sign is, is additionally provided with suction pipe in the box body.
Further, a kind of above-mentioned immunofluorescence for detecting cat serum amyloid A protein chromatographs detection card, special
Sign is that the shell is equipped with well compatible with sample pad, watch window compatible with bonding pad.
Further, a kind of above-mentioned immunofluorescence for detecting cat serum amyloid A protein chromatographs detection card, special
Sign is that the housing upper surface is additionally provided with tread plate.
Compared with prior art, technical solution provided by the utility model has the advantages that
1, the utility model operating procedure is simple, optimizes to sample pretreating method, it is only necessary to acquisition cat serum is used,
Being added in the well of detection card after dilution can be detected, and detection speed is fast, go out as a result, result can qualitative observation within 10 minutes
It can quantitative determine again, substantially reduce testing cost, reduce workload.
2, technical solution provided by the present application, by the optimization to sample pad treatment fluid and mark fluorescent microballoon dilution,
Compared to traditional handicraft, sensitivity is obviously high, and minimum detection limit (LOD) can reach 1.55mg/L (original process 3.26mg/L), linearly
More preferably, the coefficient of determination of dose-response curve is promoted by 0.9953 to 0.9996；It is reproducible, low middle high three horizontal Quality Controls
The coefficient of variation is below 10% (8.52%, 7.95% and 5.63%).
3, technical solution provided by the present application is strictly investigated to bonding pad, nitrocellulose membrane preparation process, is optimized, further
Improve the precision and reliability of detection card.
Detailed description of the invention
Fig. 1 is Test paper card structure schematic diagram provided by the utility model；
Fig. 2 is test strip structural schematic diagram provided by the utility model；
Fig. 3 is another Test paper card structure schematic diagram provided by the utility model；
Fig. 4 is schematic diagram when detection provided by the utility model card determines result for the positive；
Fig. 5 is that detection card provided by the utility model determines that result is negative schematic diagram；
Fig. 6 is that the utility model detection card provided by the utility model determines a kind of schematic diagram when result is invalid；
Fig. 7 is that the utility model detection card provided by the utility model determines another kind schematic diagram when result is invalid；
Fig. 8 is the canonical plotting of viral antigen Concentration Testing provided by the present application.
Specific embodiment
With reference to embodiment, claim of the invention is described in further detail.
Embodiment 1
A kind of immunofluorescence for detecting cat serum amyloid A protein provided by the invention chromatographs detection card, refering to fig. 1
It is equipped with the immunofluorescence for detecting cat serum amyloid A protein to Fig. 2, including shell 7, in the shell 7 and chromatographs detection
Card, the immunofluorescence chromatography detection card for detecting cat serum amyloid A protein includes bottom plate 1, on bottom plate 1 successively
Linking has sample pad 2, bonding pad 3, nitrocellulose membrane 4 and water absorption pad 5, and the bonding pad 3 is equipped with the SAA of fluorescent microsphere label
The goat-anti chicken IgY32 of monoclonal antibody 31 and fluorescent microsphere label, the nitrocellulose membrane 4 are equipped with a coating chicken IgY's
The detection T line of Quality Control C line and a coating SAA monoclonal antibody.The shell 7 is equipped with compatible with sample pad 2
Well 72, watch window 71 compatible with bonding pad 3,7 upper surface of shell are additionally provided with tread plate 73, facilitate reagent
The taking-up of card be put into.
Embodiment 2
Another immunofluorescence chromatography detection card for detecting cat serum amyloid A protein provided by the present application, refering to
Fig. 1 to Fig. 3, including box body 9, the body 9 are interior equipped with sample diluting liquid bottle 6, suction pipe 8 and for detecting cat serum amyloid
The immunofluorescence chromatography detection card of sample albumin A, the immunofluorescence chromatography detection for detecting cat serum amyloid A protein
It is positioned in shell 7, the immunofluorescence chromatography detection card for detecting cat serum amyloid A protein includes bottom plate 1, the bottom of at
Successively linking has sample pad 2, bonding pad 3, nitrocellulose membrane 4 and water absorption pad 5 on plate 1, and the bonding pad 3 is equipped with fluorescent microsphere
The goat-anti chicken IgY32 of SAA monoclonal antibody 31 and the fluorescent microsphere label of label, the nitrocellulose membrane 4 are equipped with a packet
By the Quality Control C line of chicken IgY and the detection T line of a coating SAA monoclonal antibody.The shell 7 is equipped with and sample
The compatible well 72 of pad 2, watch window 71 compatible with bonding pad 3,7 upper surface of shell are additionally provided with tread plate
73, facilitate the taking-up of reagent card and is put into.
More specifically, sample diluting liquid is containing 1%S9,1%SDS, 0.1% in the sample diluting liquid bottle
The Tris- hydrochloride buffer of the 0.1M pH7.8 of Triton X-100.
Embodiment 3
A kind of immunofluorescence for detecting cat serum amyloid A protein provided in embodiment 1 or 2 chromatographs detection card
Preparation method, assembled in the environment of temperature (18~26) DEG C, humidity≤30%, successively linking has nitre on bottom plate 1
Sour tunica fibrosa 4, water absorption pad 5, bonding pad 3 and sample pad 2；The big plate pasted is cut into the test strips of 4.0mm wide, is packed into plastics
It gets stuck in 7, i.e. SAA detection card；Each detection is placed in aluminum foil bag, desiccant 1 is added and wraps, heat sealing is spare.
Specifically:
The preparation of 1.1 bonding pads
1.1.1 the label of SAA monoclonal antibody
1) it dilutes fluorescent microsphere: taking a centrifuge tube, 1ml is added and marks buffer 0.1M MES (2- (N- morpholine) second sulphur
Acid) buffer, fluorescent microsphere 1mg is added, is vortexed and mixes；The microballoon is using the fluorescence tramp containing rare-earth europium element
Polystyrene microsphere；Density: 1.05g/cm3；Refractive index: 1.59@589nm, 25 DEG C；Diameter 300nm；Functional group: carboxyl；Outside
It sees: white.
2) 20 μ l-50 μ l label activator A and 20 μ l-50 μ l label the activation of microballoon: is added in step 1) centrifuge tube
Activator B reacts 30min on rotary incubator；
The label activator A is the MES buffer of the 0.05M of HOSu NHS containing 50mg/mLN- (NHS)
(2- (N- morpholine) ethanesulfonic acid)；The label activator B is containing 50mg/mL1- (3- dimethylamino-propyl) -3- ethyl carbon two
The 0.05M MES buffer (2- (N- morpholine) ethanesulfonic acid) of inferior amine salt hydrochlorate (EDC).
3) activation is quenched: the fluorescent microsphere 20000g after activation being centrifuged 30min, supernatant is abandoned, leaves and takes precipitating, is added
5000 μ l quenchers, ultrasonic 2s；The quencher is the MES buffer of the 0.1M containing 0.25% methanol；
4) label of antibody: 20-200 μ g SAA monoclonal antibody is added in the fluorescent microsphere after ultrasound is resuspended, is vortexed
It mixes, is placed on rotary incubator and reacts 30min；
5) it closes: 2ml is added and marks confining liquid, ultrasonic 2s, interval 5s are repeated 3 times, and ultrasound is completed to be placed on rotating and culturing
30min is reacted on device；The label confining liquid is the 0.2M glycine buffer containing 0.1% human serum albumins
6) purify: after completing above-mentioned reaction, 19000g is centrifuged 30min, sucks supernatant, leaves and takes precipitating, and it is glimmering that 2ml label is added
Light microballoon dilution, ultrasound, work 2S, and interval 5S is repeated 3 times.The fluorescent microsphere dilution contains 1% human seralbumin egg
The glycine buffer of white, 3% sucrose pH9.0 0.1M.
1.1.2 the label of Quality Control C line antibody
1) it dilutes microballoon: taking a centrifuge tube, the label buffer 0.1M MES buffer of 1ml is added, takes fluorescent microsphere
1.0mg is vortexed and mixes；The microballoon is the polystyrene microsphere of the fluorescence tramp containing rare-earth europium element；Density:
1.05g/cm3；Refractive index: 1.59@589nm, 25 DEG C；Diameter 300nm；Functional group: carboxyl；Appearance: white.
2) activation of microballoon: 20 μ l-50 μ l label activator A and 20 μ l-50 μ l label is added in above-mentioned centrifuge tube and lives
Agent B reacts 30min on rotary incubator；
The label activator A is the MES buffer of the 0.05M of HOSu NHS containing 50mg/mLN- (NHS)
(2- (N- morpholine) ethanesulfonic acid)；The label activator B is containing 50mg/mL1- (3- dimethylamino-propyl) -3- ethyl carbon two
The 0.05M MES buffer (2- (N- morpholine) ethanesulfonic acid) of inferior amine salt hydrochlorate (EDC).
3) activation is quenched: the fluorescent microsphere 19000g after activation being centrifuged 30min, abandons supernatant, leaves and takes precipitating, 2ml is added
Quencher, 90W ultrasound, work 2s, and interval 5s is repeated 1 times；The MES that the quencher is the 0.1M containing 0.25% methanol is slow
Fliud flushing；
4) label of antibody: 100 μ g goat-anti chicken IgY are added in the fluorescent microsphere after ultrasound is resuspended, is vortexed and mixes, be placed in
30min is reacted on rotary incubator；
5) it closes: 500 μ l is added and mark confining liquid, close 30min；The label confining liquid is containing 0.1% human serum
The 0.2M glycine buffer of albumin；
6) purify: after completing above-mentioned reaction, 19000g is centrifuged 30min, sucks supernatant, leaves and takes precipitating, and it is glimmering that 2ml label is added
Light microballoon dilution, 90W ultrasound, work 2s, and interval 5s is repeated 3 times, and the fluorescent microsphere dilution contains 1% human seralbumin
Albumen, 3% sucrose pH9.0 0.1M glycine buffer.
1.1.3 film is sprayed
1) glass fiber is cut into (10 ± 1) mm × (300 ± 10) mm size is bonding pad；
2) T, C line antibody after label are uniformly mixed by 20:1 (V:V), 90W ultrasound, work 2s, interval 5s, repeats 3
It is secondary；
3) it is sprayed on the bonding pad after cutting out by 3.5 μ l/cm, 0.02MPa；
4) after having sprayed, (16~18) h is dried overnight in 37 DEG C of drying boxes.
The preparation of 1.2 reaction films
1) chicken IgY is diluted to 1mg/mL with the phosphate buffer (PBS) of the 0.01M of the pH 7.4 containing 3% sucrose, i.e.,
For C line working solution.
2) use the phosphate buffer (PBS) of the 0.01M of the pH 7.4 containing 3% sucrose by another plant of SAA monoclonal antibody
It is diluted to 1mg/mL, as T line working solution.
3) PVC bottom plate is taken, nitrocellulose membrane is pasted.
4) T line and C line are drawn on nitrocellulose membrane, scribing line concentration is 1 μ L/cm.
5) sheet material is placed in 37 DEG C of drying boxes after the completion of and is dried overnight (16~18) h.
The preparation of 1.3 sample pads
1) prepare sample pad treatment fluid: containing 0.1% human serum albumins, 0.5% N of IgG, 2% Macrogol 4000,
The 0.2M pH9.0 borate buffer solution of 0.3%Triton X-100,4% sodium chloride and 0.05%Prolin 300.
2) buffer is sprayed on glass fibre with the concentration of 10 μ l/cm.
Detection method
For the ease of using detection provided by the present application to block, detection card provided by the present application is given below, two kinds of detections are provided
Method.
Method one: using ultraviolet light irradiation, as a result refering to Fig. 4 to Fig. 7: hand-held ultraviolet light irradiation watch window is used,
When nature controlling line and the detection line amount of having fluorescence occur (Fig. 4), illustrate that SAA antigen is the positive in sample；When only nature controlling line has
When fluorescence and detection line unstressed configuration occur (Fig. 5), illustrate that SAA antigen is feminine gender in sample；Nature controlling line does not occur fluorescence (Fig. 6, figure
7) it is invalid, then to represent the wrong or testing result of operation, need to repeat to test.
Method two: Immunofluorescence test instrument testing result prepared by the embodiment of the present application 3:
1) cat serum is collected；
2) 10 μ l is taken to be added containing 1.0ml sample diluting liquid (containing 1%S9,1%SDS, 0.1%Triton X-100
The Tris- hydrochloride buffer of 0.1M pH7.8) sample cell in, mix well；
3) detection card is taken out, dry type Immunofluorescence test instrument is opened；
4) the 75 μ l of sample after dilution is drawn, is added in the well of detection card, will test the card that card is put into instrument
In slot, start timing；
5) after 10min, " test " key on instrument is clicked, instrument will start to test；
6) fluorescence intensity is directly proportional to the SAA concentration in sample, is carried out curve fitting by built-in standard curve and concentration
Calculating, dose-effect curve Log (Y)=0.6706Log (X) -0.5366, R=0.9996 (R2=0.9998), refering to Fig. 3.
Immunofluorescence test instrument testing result:
1, the range of linearity
1.1.1 SAA calibration object is diluted to respectively with negative serum 0mg/L, 5mg/L, 8mg/L, 12mg/L, 20 mg/L,
40mg/L, 80mg/L, 120mg/L, 200mg/L, 300mg/L, 400mg/L, 500mg/L, 600mg/L, 700mg/L and
800mg/L；
1.1.2 above each concentration samples are taken into 75 μ L respectively, every concentration into the cat SAA detection reagent prepared is added
Retest 2 times；
1.1.3 after reacting 10min, test paper is put into dry type immunofluorescence analysis instrument, T/C value is read, passes through built-in mark
The calculating that directrix curve carries out curve fitting with concentration.
1.1.4 test result is shown: reagent is preferable in the SAA linear fit relationship for detecting 5~300mg/L, r >
0.9900, when SAA concentration increases to 400mg/L, linear fit r value slows down less than 0.9900, T/C growth, SAA 400~
T/C value is gentle when 500mg/L, and when SAA reaches 800mg/L, T/C value reduces instead.Therefore the maximum detection range of this detection can
To reach 400mg/L.
2, minimum detectability
By negative serum, retest 20 times, T/C mean value is calculated, is substituted into dose-response curve, obtains we
The minimum detectability of method is 1.55mg/L, is specifically shown in Table 1 and Fig. 8；
Table 1
3, precision
SAA calibration object is diluted to 20mg/L, 60mg/L, 150mg/L with negative serum, blocks every concentration with this method detection
Retest 10 times, calculate the coefficient of variation of test concentrations.As a result shown in table 2, it can thus be seen that the change of this kit detection
Different coefficient is respectively less than 10% (three concentration are respectively 8.52%, 7.95% and 5.63%), therefore this detection method is with higher
Precision.
Table 2
| Concentration | The coefficient of variation (CV) | |
| Quality Control I | 20mg/L | 8.52% |
| Quality Control II | 60mg/L | 7.95% |
| Quality Control III | 150mg/L | 5.63% |
The beneficial effect of type in order to better illustrate the present invention, be given below using type of the present invention provide detection reagent and
Detection performance energy comparing result of traditional immunoturbidimetry, immunochromatographic method when detecting SAA.
Table 3
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
Claims (5)
1. a kind of immunofluorescence for detecting cat serum amyloid A protein chromatographs detection card, including shell (7), the shell
It is equipped with the immunofluorescence for detecting cat serum amyloid A protein in body (7) and chromatographs detection card, it is described for detecting cat serum shallow lake
The immunofluorescence chromatography detection card of powder sample albumin A includes bottom plate (1), and successively linking has sample pad (2), combines on bottom plate (1)
Pad (3), nitrocellulose membrane (4) and water absorption pad (5), which is characterized in that the bonding pad (3) is equipped with fluorescent microsphere label
The goat-anti chicken IgY (32) of SAA monoclonal antibody (31) and fluorescent microsphere label, the nitrocellulose membrane (4) are equipped with a packet
By the Quality Control C line of chicken IgY and the detection T line of a coating SAA monoclonal antibody.
2. a kind of immunofluorescence for detecting cat serum amyloid A protein according to claim 1 chromatographs detection card,
It is characterized in that, further includes box body (9), sample dilution bottle (6) are equipped in the box body (9) and for detecting cat serum amyloid sample egg
The immunofluorescence chromatography detection card of white A.
3. a kind of immunofluorescence for detecting cat serum amyloid A protein according to claim 2 chromatographs detection card,
It is characterized in that, is additionally provided with suction pipe (8) in the box body (9).
4. a kind of immunofluorescence for detecting cat serum amyloid A protein according to claim 1 chromatographs detection card,
It is characterized in that, the shell (7) is equipped with sample pad (2) compatible well (72), compatible with bonding pad (3)
Watch window (71).
5. a kind of immunofluorescence for detecting cat serum amyloid A protein according to claim 1 chromatographs detection card,
It is characterized in that, the shell (7) upper surface is additionally provided with tread plate (73).
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110618266A (en) * | 2019-09-26 | 2019-12-27 | 河北省科学院生物研究所 | Kit for detecting mastitis of dairy cow and use method thereof |
| CN112798778A (en) * | 2021-03-17 | 2021-05-14 | 广州敏捷生物技术有限公司 | Immunofluorescence chromatography detection card and method for synchronously detecting florfenicol and trimethoprim contents in poultry eggs |
| CN117805402A (en) * | 2024-03-01 | 2024-04-02 | 北京纳百生物科技有限公司 | Cat blood typing detection method and kit |
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2018
- 2018-05-04 CN CN201820668071.2U patent/CN208443851U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110618266A (en) * | 2019-09-26 | 2019-12-27 | 河北省科学院生物研究所 | Kit for detecting mastitis of dairy cow and use method thereof |
| CN112798778A (en) * | 2021-03-17 | 2021-05-14 | 广州敏捷生物技术有限公司 | Immunofluorescence chromatography detection card and method for synchronously detecting florfenicol and trimethoprim contents in poultry eggs |
| CN117805402A (en) * | 2024-03-01 | 2024-04-02 | 北京纳百生物科技有限公司 | Cat blood typing detection method and kit |
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