CN108593633A - A kind of Test paper for quickly detecting saliva uric acid - Google Patents
A kind of Test paper for quickly detecting saliva uric acid Download PDFInfo
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- CN108593633A CN108593633A CN201810355200.7A CN201810355200A CN108593633A CN 108593633 A CN108593633 A CN 108593633A CN 201810355200 A CN201810355200 A CN 201810355200A CN 108593633 A CN108593633 A CN 108593633A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
Abstract
The present invention relates to a kind of Test papers for quickly detecting saliva uric acid, including plastic cards paper and the indicator paper block for being pasted on plastic cards paper one end；Enzyme chromogenic reagent solution is impregnated on the indicator paper block.The Test paper is made by being impregnated with the indicator paper block of enzyme chromogenic reagent solution in the stickup of plastic cards paper one end；Simple process and low cost is conducive to extensive go into operation.In addition, since detection process is based primarily upon the half-quantitative detection of the chromogenic reaction principle realization uric acid concentration of coupling terminal colorimetric analysis, has good stability under the conditions of 35 DEG C, independently carried convenient for patient and carry out uric acid monitoring, the state of an illness is found in time conducive to patient, selects best occasion for the treatment.The Test paper is equally applicable to the monitoring of urine uric acid.
Description
Technical field
The present invention relates to uric acid Test paper, more particularly to a kind of Test papers for quickly detecting saliva uric acid.It should
Chromogenic reaction principle of the Test paper based on coupling terminal colorimetric analysis is made, being capable of rapid semi-quantitative detection uric acid in the saliva
Concentration.
Background technology
It is developed rapidly recently as China's economy, significant change, egg also has occurred in improvement of living standard, dietary structure
White matter and food intake dose rich in purine ingredient increase, and the illness rate of hyperuricemia increases year by year, the trouble of high lithemia nephrosis
Sick rate is consequently increased.Lot of domestic and foreign research confirms hyperuricemia and gout, angiocardiopathy, metabolic syndrome, high blood
The generation of pressure and kidney trouble is closely related, is the independent hazard factor of these disease developments.Therefore, pay attention to the inspection of uric acid
It surveys, for preventing above-mentioned disease in time, corresponding treatment measure is taken to have very important significance.Uric acid concentration is detected at present
Method is mainly that hospital is detected with automatic clinical chemistry analyzer；This is detected as invasive blood collection, and patient is not easily accepted by, in particular for
Dynamic detection uric acid patient is even more that can not be difficult to detect.The detection of uric acid concentration can also coordinate electricity raw by uric acid Test paper
Change equipment to complete, but this method is bad and expensive to the comprehensive latter stage anuria patient outcome of nephrosis.
It is reported according to foreign literature, since the source of saliva, composition, function and other organs are there are interaction relationship,
Salivary analysis has become one of the most important diagnosis methods that diagnose the illness.Saliva has than serum more as a kind of diagnosticum
Unique advantage, such as noninvasive, patient are easier receiving, many ingredients in saliva the inside have splendid correlation with blood, can drop
The potential risk etc. of low disease infection.Saliva uric acid level can reflect the variation of respective substance in blood, and with good
Correlation.J. Zhao Research Teams show that the ratio of saliva uric acid and serum uric acid is 0.83 ± 0.11, this is examined for saliva uric acid
Survey provides most important theoretical foundation.Patent CN IO5445449A provide one kind by one carrier protein of solid phase uric acid and sheep
The nitrocellulose filter of anti-rabbit IgG polyclonal antibodies, the glass fibre for being adsorbed with the anti-uric acid antibody of colloid gold label, sample pad,
Other auxiliary materials such as blotting paper paste manufactured paper chip, the advantage is that structure novel, high specificity can rapid semi-quantitative detection
Saliva uric acid content；But manufacture craft difficulty is big, is not easy to go into operation on a large scale.Patent CN 206292240U are proposed can be square
Just the quick high-throughput Test paper of detection multi objective only needs a sample of bleeding that can quickly measure multinomial physiology in blood
Index, but the test paper must could be realized with Miniature hand-held Instrument crosslinking.
Invention content
The deficiencies of complicated, invasive and expensive for uric acid detection process at this stage, the present invention provides one kind to be used for
The quickly Test paper of detection saliva uric acid, which includes the indicator paper block for being impregnated with enzyme chromogenic reagent solution, once uric acid
It is contacted with indicator paper block, the enzyme chromogenic reagent solution on indicator paper block will be reacted with uric acid, according to coupling terminal colorimetric analysis chromogenic reaction
Principle generates quinone-imine compound, and the depth and uric acid content of quinone-imine compound color depth are proportional, and because of saliva
The correlative relationship of uric acid concentration and serum uric acid concentration, therefore can further judge serum by indicator paper block color change
Uric acid concentration provides safer, economic, easily means for monitoring health.
A kind of Test paper for quickly detecting saliva uric acid, including plastic cards paper and it is pasted on plastic cards paper one
The indicator paper block at end is impregnated with enzyme chromogenic reagent solution on the indicator paper block.
Further, the enzyme chromogenic reagent solution includes uricase, horseradish peroxidase, 4- amino for than woods, 3- hydroxyls
Base -2,4,6- tribromo benzoic acid（TBHBA）With bis- chloro-5-trifluoromethylpyridines of 2,3-.
Further, the enzyme chromogenic reagent solution further includes bilirubin oxidase.
Further, the enzyme chromogenic reagent solution further includes gelatin solution, a concentration of 1.6 mg/ of institute's gelatine solution
mL-2.0 mg/mL。
Further, each constituent concentration is as follows in the enzyme chromogenic reagent solution：
A concentration of 0.15 KU/L-0.3 KU/L of uricase；
A concentration of 2.0 KU/L-3.5 KU/L of horseradish peroxidase；
4- amino replaces 100 mg/L more a concentration of than woods；
A concentration of 4 g/L-6 g/L of 3- hydroxyl -2,4,6- tribromo benzoic acid；
A concentration of 1.5 g/L-2 g/L of bis- chloro-5-trifluoromethylpyridines of 2,3-；
A concentration of 0.05 g/L-0.2 g/L of bilirubin oxidase；
Gelatin solution is 0.5 mL.
Further, the enzyme chromogenic reagent solution uses borate standard buffer solution as lysate, the borate
The pH value of standard buffer solution is 8.5.
Further, the indicator paper block is middling speed quantitative filter paper or cellulose paper.
A kind of Test paper preparation method for quickly detecting saliva uric acid includes the following steps：
The preparation of S1, gelatin solution, 2 h of addition appropriate amounts of gelatin foaming, molten under the conditions of being subsequently placed at 60 DEG C first in ultra-clean water
Solution at a concentration of 1.6 mg/mL-2.0 mg/mL gelatin solution；
The preparation of S2, enzyme chromogenic reagent solution, using pH value be 8.5 borate standard buffer solution as lysate, be separately added into
Suitable uricase, horseradish peroxidase, 4- amino are replaced than woods, 3- hydroxyl -2,4,6- tribromos benzoic acid, bis- chloro- 5- of 2,3-
Trifluoromethyl pyridine, bilirubin oxidase and gelatin solution form enzyme chromogenic reagent solution after mixing；
S3, indicator paper block is sufficiently impregnated the 30-40 min in enzyme chromogenic reagent solution be placed under cryogenic conditions air-dry, then seal
It is protected from light cold deposit；
S4, one end that the indicator paper block for passing through maceration enzyme chromogenic reagent solution in step S3 is pasted onto to plastic cards paper, low-temperature air-drying
It can be obtained the Test paper for quickly detecting saliva uric acid afterwards.
Further, each constituent concentration is as follows in the enzyme chromogenic reagent solution that step S2 is prepared：
A concentration of 0.15 KU/L-0.3 KU/L of uricase；
A concentration of 2.0 KU/L-3.5 KU/L of horseradish peroxidase；
4- amino replaces 100 mg/L more a concentration of than woods；
A concentration of 4 g/L-6 g/L of 3- hydroxyl -2,4,6- tribromo benzoic acid；
A concentration of 1.5 g/L-2 g/L of bis- chloro-5-trifluoromethylpyridines of 2,3-；
A concentration of 0.05 g/L-0.2 g/L of bilirubin oxidase；
Gelatin solution is 0.5 mL.
Further, the indicator paper block is 5 mm X, 5 mm, and the indicator paper block passes through sticking double faced adhesive tape with plastic cards paper.
The advantageous effects that the present invention is played are as follows：
（1）Test paper disclosed by the invention for quickly detecting saliva uric acid is impregnated by being pasted in plastic cards paper one end
There is the indicator paper block of enzyme chromogenic reagent solution to be made；Simple process and low cost is conducive to extensive go into operation.
（2）Test paper disclosed by the invention for quickly detecting saliva uric acid is based primarily upon coupling terminal colorimetric analysis
Chromogenic reaction principle realizes the half-quantitative detection of uric acid concentration, has good stability under the conditions of 35 DEG C, is independently carried convenient for patient
Uric acid monitoring is carried out, the state of an illness is found in time conducive to patient, selects best occasion for the treatment.
（3）Test paper disclosed by the invention for quickly detecting saliva uric acid has higher sensitivity, equally applicable
In the monitoring of urine uric acid.
Description of the drawings
Fig. 1 is Test paper structural schematic diagram of the present invention for quickly detecting saliva uric acid.
Reference sign：
1- plastic cards paper, 2- indicator paper blocks.
Specific implementation mode
The preferred embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings, so that advantages and features of the invention are more
It is easily readily appreciated by one skilled in the art, to make apparent define to protection scope of the present invention.
Embodiment 1：
The present embodiment provides a kind of Test papers for quickly detecting saliva uric acid, including plastic cards paper 1 and are pasted on modeling
Expect the indicator paper block 2 of 1 one end of ivory board, enzyme chromogenic reagent solution is impregnated on the indicator paper block 2, during the indicator paper block 2 can be selected
Any one of fast quantitative filter paper or cellulose paper, indicator paper block 2 selects middling speed quantitative filter paper, the indicator paper block in the present embodiment
2 sizes are 5 mm X, 5 mm, and the indicator paper block 2 passes through sticking double faced adhesive tape with plastic cards paper 1.Wherein, the enzyme developer solution
For liquid using borate standard buffer solution as lysate, the pH value of the borate standard buffer solution is 8.5.Enzyme color developing agent
Solution specifically includes uricase, horseradish peroxidase, 4- amino and replaces than woods, 3- hydroxyls -2,4,6- tribromos benzoic acid and 2,3-
Two chloro-5-trifluoromethylpyridines, and concentration control of the above-mentioned each ingredient in enzyme chromogenic reagent solution is as follows：Uricase is a concentration of
0.15 KU/L-0.3 KU/L；A concentration of 2.0 KU/L-3.5 KU/L of horseradish peroxidase；4- amino is for more a concentration of than woods
100 mg/L；A concentration of 4 g/L-6 g/L of 3- hydroxyl -2,4,6- tribromo benzoic acid；Bis- chloro-5-trifluoromethylpyridines of 2,3- are dense
Degree is 1.5 g/L-2 g/L.Preferred concentration of the above-mentioned each component in enzyme chromogenic reagent solution is as follows：Uricase a concentration of 0.23
KU/L；A concentration of 2.7 KU/L of horseradish peroxidase；4- amino replaces 100 mg/L more a concentration of than woods；3- hydroxyls -2,4,6- three
A concentration of 5 g/L of bromobenzoic acid；A concentration of 1.8 g/L of bis- chloro-5-trifluoromethylpyridines of 2,3-.
The chromogenic reaction that the above-mentioned Test paper for quickly detecting saliva uric acid is based primarily upon coupling terminal colorimetric analysis is former
Reason realizes the half-quantitative detection of uric acid concentration, and chromogenic reaction principle is specific as follows：
（1）Uric acid+O2+H2OUricaseAllantoin+CO2+H2O2；
（2）H2O2+ 4-AA+TBHBAPeroxidaseQuinoneimine dye+H2O；
Once uric acid is contacted with indicator paper block 2, the enzyme chromogenic reagent solution on indicator paper block 2 will be reacted with uric acid, according to coupling terminal
Colorimetric method chromogenic reaction principle generates quinone-imine compound, and the depth of quinone-imine compound color depth is directly proportional to uric acid content
Relationship, and because of the correlative relationship of saliva uric acid concentration and serum uric acid concentration, thus can by 2 color change of indicator paper block,
And then judge serum uric acid concentration.
The half-quantitative detection process of uric acid is as follows in saliva：
Test paper described in the present embodiment mainly utilizes the depth that the uric acid concentration in saliva develops the color with Test paper at just
Saliva uric acid is measured than relationship, while again because there are certain correlations with human serum uric acid for the uric acid in saliva, it is meant that
Serum uric acid can be calculated according to relationship between the two.Therefore, the half-quantitative detection process of uric acid needs first in saliva
A series of standard color comparison card is prepared according to the correlation of uric acid and uric acid in serum in saliva.Then, ensuring detection pair
Under conditions of having been cleaned out as oral cavity content, 20 μ L salivas is taken to drip on manufactured Test paper in the present embodiment, and
The color change that Test paper in 1 min is observed under normal temperature environment, by the color change of Test paper and standard color comparison card pair
Than that can understand the approximate range of serum uric acid concentration, complete the half-quantitative detection of serum uric acid.Detection method safety, warp
It helps, is convenient, certain directive significance is provided the health examination of patient.
Embodiment 2：
The present embodiment is similar with embodiment, and further, the enzyme chromogenic reagent solution specifically includes uricase, horseradish hydrogen peroxide
Enzyme, 4- amino are replaced than woods, 3- hydroxyl -2,4,6- tribromos benzoic acid, bis- chloro-5-trifluoromethylpyridines of 2,3-, bilirubin oxidase
And gelatin solution, a concentration of 1.6 mg/mL-2.0 mg/mL of institute's gelatine solution.Above-mentioned each component is in enzyme chromogenic reagent solution
Content it is as follows：A concentration of 0.15 KU/L-0.3 KU/L of uricase；A concentration of 2.0 KU/L-3.5 of horseradish peroxidase
KU/L；4- amino replaces 100 mg/L more a concentration of than woods；A concentration of 4 g/L-6 g/L of 3- hydroxyl -2,4,6- tribromo benzoic acid；2,
A concentration of 1.5 g/L-2 g/L of bis- chloro-5-trifluoromethylpyridines of 3-；A concentration of 0.05 g/L-0.2 g/ of bilirubin oxidase
L；Gelatin solution is 0.5 mL.In the present embodiment, preferred concentration of each component in enzyme chromogenic reagent solution is as follows：Uricase is dense
Degree is 0.23 KU/L；A concentration of 2.7 KU/L of horseradish peroxidase；4- amino replaces 100 mg/L more a concentration of than woods；3- hydroxyls-
A concentration of 5 g/L of 2,4,6- tribromo benzoic acid；A concentration of 1.8 g/L of bis- chloro-5-trifluoromethylpyridines of 2,3-；Bilirubin aoxidizes
A concentration of 1.2 g/L of enzyme；Gelatin solution is 0.5 mL.
Embodiment 3：
A kind of Test paper preparation method for quickly detecting saliva uric acid is present embodiments provided, following step is specifically included
Suddenly：
The preparation of S1, gelatin solution, 2 h of addition appropriate amounts of gelatin foaming, molten under the conditions of being subsequently placed at 60 DEG C first in ultra-clean water
Solution is at the gelatin solution of a concentration of 1.6 mg/mL-2.0 mg/mL, concentration preferably 1.8 mg/mL of gelatin solution；
The preparation of S2, enzyme chromogenic reagent solution, using pH value be 8.5 borate standard buffer solution as lysate, be separately added into
Suitable uricase, horseradish peroxidase, 4- amino are replaced than woods, 3- hydroxyl -2,4,6- tribromos benzoic acid, bis- chloro- 5- of 2,3-
Trifluoromethyl pyridine, bilirubin oxidase and gelatin solution form enzyme chromogenic reagent solution after mixing；
S3, indicator paper block 2 is sufficiently impregnated the 30-40 min in enzyme chromogenic reagent solution be placed under cryogenic conditions air-dry, it is then close
Envelope be protected from light it is cold deposit, in the present embodiment, it is best that 2 size of the indicator paper block, which is cut into 5 mm X, 5 mm,；
S4, one end that the indicator paper block 2 for passing through maceration enzyme chromogenic reagent solution in step S3 is pasted onto to plastic cards paper 1, low temperature wind
It can be obtained the Test paper for quickly detecting saliva uric acid after dry.In the present embodiment, the indicator paper block 2 and plastic cards paper
1 passes through sticking double faced adhesive tape.
Wherein, it is as follows that the enzyme chromogenic reagent solution being prepared in step S2 contains each constituent concentration：
Uricase a concentration of 0.15 KU/L-0.3 KU/L, preferably 0.23 KU/L；
Horseradish peroxidase a concentration of 2.0 KU/L-3.5 KU/L, preferably 2.7 KU/L；
4- amino replaces 100 mg/L more a concentration of than woods；
3- hydroxyls -2,4, a concentration of 4 g/L-6 g/L of 6- tribromo benzoic acid, preferably 5 g/L；
2,3- bis- chloro-5-trifluoromethylpyridines a concentration of 1.5 g/L-2 g/L, preferably 1.8 g/L；
A concentration of 0.05 g/L-0.2 g/L of bilirubin oxidase, preferably 1.2 g/L；
Gelatin solution is 0.5 mL.
The present embodiment is during preparing the Test paper for quickly detecting saliva uric acid, in order to preferably by test paper
Block 2 is sufficiently impregnated in enzyme chromogenic reagent solution, can impregnated paper base material be first cut into test paper according to 5 mm X, 8 cm specifications
Item makes all to be immersed in enzyme chromogenic reagent solution inside test strips, and soaking time is taken out after keeping 30-40 min, and preferably 40
Then min air-dries under cryogenic conditions, test strips is finally cut into the indicator paper block 2 of 5 mm X, 5 mm sizes again, sealing is protected from light
It is cold to deposit for use.
Embodiments of the present invention are explained in detail above in conjunction with attached drawing, but the present invention is not limited to above-mentioned implementations
Mode within the knowledge of a person skilled in the art can also be without departing from the purpose of the present invention
Various changes can be made.
Claims (10)
1. a kind of Test paper for quickly detecting saliva uric acid, which is characterized in that including plastic cards paper（1）And it is pasted on
Plastic cards paper（1）The indicator paper block of one end（2）, the indicator paper block（2）On be impregnated with enzyme chromogenic reagent solution.
2. a kind of Test paper for quickly detecting saliva uric acid as described in claim 1, which is characterized in that the enzyme colour developing
Agent solution includes uricase, horseradish peroxidase, 4- amino for than woods, 3- hydroxyls -2,4,6- tribromos benzoic acid and 2,3- bis-
Chloro-5-trifluoromethylpyridine.
3. a kind of Test paper for quickly detecting saliva uric acid as claimed in claim 2, which is characterized in that the enzyme colour developing
Agent solution further includes bilirubin oxidase.
4. a kind of Test paper for quickly detecting saliva uric acid as described in any one of Claims 2 or 3, which is characterized in that
The enzyme chromogenic reagent solution further includes gelatin solution, a concentration of 1.6 mg/mL-2.0 mg/mL of institute's gelatine solution.
5. a kind of Test paper for quickly detecting saliva uric acid as claimed in claim 4, which is characterized in that the enzyme colour developing
Each constituent concentration is as follows in agent solution：
A concentration of 0.15 KU/L-0.3 KU/L of uricase；
A concentration of 2.0 KU/L-3.5 KU/L of horseradish peroxidase；
4- amino replaces 100 mg/L more a concentration of than woods；
A concentration of 4 g/L-6 g/L of 3- hydroxyl -2,4,6- tribromo benzoic acid；
A concentration of 1.5 g/L-2 g/L of bis- chloro-5-trifluoromethylpyridines of 2,3-；
A concentration of 0.05 g/L-0.2 g/L of bilirubin oxidase；
Gelatin solution is 0.5 mL.
6. a kind of Test paper for quickly detecting saliva uric acid as described in claim 1, which is characterized in that the enzyme colour developing
For agent solution using borate standard buffer solution as lysate, the pH value of the borate standard buffer solution is 8.5.
7. a kind of Test paper for quickly detecting saliva uric acid as described in claim 1, which is characterized in that the indicator paper block
（2）For middling speed quantitative filter paper or cellulose paper.
8. a kind of Test paper preparation method for quickly detecting saliva uric acid, which is characterized in that include the following steps：
The preparation of S1, gelatin solution, 2 h of addition appropriate amounts of gelatin foaming, molten under the conditions of being subsequently placed at 60 DEG C first in ultra-clean water
Solution at a concentration of 1.6 mg/mL-2.0 mg/mL gelatin solution；
The preparation of S2, enzyme chromogenic reagent solution, using pH value be 8.5 borate standard buffer solution as lysate, be separately added into
Suitable uricase, horseradish peroxidase, 4- amino are replaced than woods, 3- hydroxyl -2,4,6- tribromos benzoic acid, bis- chloro- 5- of 2,3-
Trifluoromethyl pyridine, bilirubin oxidase and gelatin solution form enzyme chromogenic reagent solution after mixing；
S3, by indicator paper block（2）Be sufficiently impregnated the 30-40 min in enzyme chromogenic reagent solution be placed under cryogenic conditions air-dry, then
Sealing is protected from light cold deposit；
S4, the indicator paper block that maceration enzyme chromogenic reagent solution will be passed through in step S3（2）It is pasted onto plastic cards paper（1）One end, it is low
It can be obtained the Test paper for quickly detecting saliva uric acid after warm air is dry.
9. a kind of Test paper preparation method for quickly detecting saliva uric acid as claimed in claim 8, which is characterized in that step
Each constituent concentration is as follows in the enzyme chromogenic reagent solution that rapid S2 is prepared：
A concentration of 0.15 KU/L-0.3 KU/L of uricase；
A concentration of 2.0 KU/L-3.5 KU/L of horseradish peroxidase；
4- amino replaces 100 mg/L more a concentration of than woods；
A concentration of 4 g/L-6 g/L of 3- hydroxyl -2,4,6- tribromo benzoic acid；
A concentration of 1.5 g/L-2 g/L of bis- chloro-5-trifluoromethylpyridines of 2,3-；
A concentration of 0.05 g/L-0.2 g/L of bilirubin oxidase；
Gelatin solution is 0.5 mL.
10. a kind of Test paper preparation method for quickly detecting saliva uric acid as claimed in claim 8, which is characterized in that
The indicator paper block（2）For 5 mm X, 5 mm, the indicator paper block（2）With plastic cards paper（1）Pass through sticking double faced adhesive tape.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110208429A (en) * | 2019-07-09 | 2019-09-06 | 延边大学 | Uric acid does paper disk method detection kit in a kind of human saliva |
| CN111077143A (en) * | 2019-12-24 | 2020-04-28 | 杭州启棣生物技术有限公司 | Uric acid detection method and device |
| CN115201186A (en) * | 2022-07-14 | 2022-10-18 | 天宇华宏(北京)医学科技股份有限公司 | Test strip for detecting uric acid in saliva and preparation method thereof |
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