CN106596535A - Blood uric acid detection test paper - Google Patents
Blood uric acid detection test paper Download PDFInfo
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- CN106596535A CN106596535A CN201611137650.6A CN201611137650A CN106596535A CN 106596535 A CN106596535 A CN 106596535A CN 201611137650 A CN201611137650 A CN 201611137650A CN 106596535 A CN106596535 A CN 106596535A
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- uric acid
- test paper
- liquid
- blood uric
- concentration
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
Abstract
The invention discloses a blood uric acid detection test paper. The test paper is produced through immersing a filter paper in a liquid A, drying the immersed filter paper, immersing the dried filter paper in a liquid B, and drying the re-immersed filter paper, wherein the liquid A comprises a PBS buffer solution, urate oxidase, peroxidase, 4-aminoantipyrine and a surfactant; and the liquid B comprises 2-(3,5-dimethoxy-4-hydroxyphenyl)-4-ethoxyphenyl-5-[4-(dimethylamino)phenyl]-imidazole and an alcohol solvent. The blood uric acid detection test paper allows the urate oxidase to rapidly react with uric acid under an alkaline condition to generate hydrogen peroxide, and the hydrogen peroxide oxidizes a colorless dye into a colored dye under the action of peroxidase, so the test paper has an obvious color interval from colorlessness to dark blue color, is easy to visually distinguish, is machine-readable, and is not interfered by other substances in blood; and the test paper also has the advantages of simple production method, easiness in operation, stable performances and accurate detection result.
Description
Technical field
The present invention relates to in-vitro diagnosis Test paper, more particularly to a kind of blood uric acid Test paper.
Background technology
Uric acid is the product during purine metabolism, under normal circumstances 70% uric acid metabolism ex vivo,
30%, from diet, is discharged by the final major part of blood circulation by kidney, and any link goes wrong in the approach, all may
Cause metabolic arthritis.Research finds that gout is clearly related to the rising of serum uric acid level.Without gout symptom hyperuricemia
In (Hyperuricemia, HUA) crowd, blood uric acid is 4.9%, 7-9mg/dl groups more than the annual morbidity of 9mg/dl group gouts
It is only 0.1% with the following group that annual morbidity is 0.5%, 7mg/dl, and patient with gout blood uric acid is down to into below 6mg/dl, gout outbreak
Can reduce with the deposition of intraarticular crystal.
In recent years research finds that gout is closely related with metabolism syndrome, and patient with gout merges the ratio of metabolism syndrome is
3.05 times of non-patient with gout.The related HUA of gout is simultaneously diabetes, chronic nephropathy, obesity, hypertension, renal calculuss, the heart
The risk factor of various metabolism related diseases such as stalk, heart failure and apoplexy.Gout is the independence of All-cause death and cardiovascular death
Risk factor, it significantly reduces the life quality of patient.In view of the heavy Disease Spectrum of gout and may caused by it is serious
The importance of the long-period of management of harm, gout and HUA increasingly causes extensive concern.But the uric acid of gout patients at this stage
Detection, it is most of all still to go to hospital to be detected in acute attack stage, it is so late, can bring greatly to patient
Pain, if it is possible to have a instrument for allowing gout patients to be capable of monitor in real time itself uric acid content, this will bring to patient
Greatly facilitate.
At present, blood uric acid is mainly detected in large-scale biochemistry analyzer.Be present problems in the method, such as take out
Blood volume is more, and the waiting time is longer.
The content of the invention
For weak point of the prior art, the purpose of this case is that offer one kind being capable of fast and easy detection blood urine
The reagent paper of acid content, this reagent paper can quickly measure uric acid concentration with Miniature hand-held Instrument crosslinking；The reagent paper need not be taken out
A large amount of venous blood are taken, micro finger tip blood is only needed, you can uric acid in blood concentration is detected within a few minutes.
For achieving the above object, technical scheme is summarized as follows：
A kind of blood uric acid Test paper, its by filter paper is immersed successively A liquid, drying, immersion B liquid, dry again it is prepared；
Wherein：
The A liquid is included：PBS, urate oxidase, peroxidase, 4-AA and surface are lived
Property agent；
The B liquid is included：2- (3,5- dimethoxy-4 's-hydroxyphenyl) -4- ethoxyphenyl -5- [4- (dimethylamino) benzene
Base]-imidazoles and alcohols solvent.
Preferably, described blood uric acid Test paper, wherein, the concentration of the urate oxidase is 30000-
50000U/L。
Preferably, described blood uric acid Test paper, wherein, the concentration of the peroxidase is 30000-
50000U/L。
Preferably, described blood uric acid Test paper, wherein, the concentration of the 4-AA is 4-5g/L.
Preferably, described blood uric acid Test paper, wherein, the concentration of the surfactant is 15-30g/L.
Preferably, described blood uric acid Test paper, wherein, 2- (3,5- dimethoxy-4 's-the hydroxyphenyl) -4- second
The concentration of oxygen phenyl -5- [4- (dimethylamino) phenyl]-imidazoles is 4-5g/L.
Preferably, described blood uric acid Test paper, wherein, the pH of the A liquid is 8.0-9.0.
Preferably, described blood uric acid Test paper, wherein, the alcohols solvent is selected from methanol or ethanol.
Preferably, described blood uric acid Test paper, wherein, the B liquid also includes the chloro- 5- trifluoromethyls of 2,3- bis-
Pyridine.
Preferably, described blood uric acid Test paper, wherein, the concentration of the chloro-5-trifluoromethylpyridines of 2, the 3- bis-
For 1.5-2.0g/L.
The invention has the beneficial effects as follows：Compared with the prior art, prior art detection blood uric acid is generally using full-automatic raw
Change analyser, the method needs blood volume larger, and the waiting time is longer, need large hospital to detect.Also there is electrochemical process in addition
Deng, but the production technology of agents useful for same bar is cumbersome, and relatively costly, test is not sufficiently stable.It is an advantage of the invention that：This case
Blood uric acid Test paper, in the basic conditions, urate oxidase quickly reacts generation hydrogen peroxide with uric acid, hydrogen peroxide
In the presence of peroxidase by colourless dye oxidation be colored dyes, reagent paper present from colourless to navy blue obvious color
Rank, it is easy to visually differentiate and machine-readable, and not disturbed by other materials in blood, method for preparing test paper simply, easy to operate, performance
Stable, measurement result is accurate.
Description of the drawings
Fig. 1 is the canonical plotting of the serum Uric Acid Concentration and absorbance obtained using the reagent paper of single chromogen.
Fig. 2 is the standard curve of the serum Uric Acid Concentration and absorbance obtained using the reagent paper of single chromogen+auxiliary chromogen
Figure.
Specific embodiment
With reference to embodiment, the present invention is described in further detail, to make those skilled in the art with reference to description
Word can be implemented according to this.
The blood uric acid Test paper of an embodiment is listed in this case, and it is by the way that filter paper to be immersed successively A liquid, drying, immersion B
Liquid, dry again it is prepared；Wherein：
A liquid is included：PBS, urate oxidase, peroxidase, 4-AA and surfactant；
B liquid is included：2- (3,5- dimethoxy-4 's-hydroxyphenyl) -4- ethoxyphenyl -5- [4- (dimethylamino) phenyl] -
Imidazoles and alcohols solvent.2- (3,5- dimethoxy-4 's-hydroxyphenyl) -4- ethoxyphenyl -5- [4- (dimethylamino) phenyl]-imidazoles
It is chromogen.
Wherein, the concentration of urate oxidase is preferably 30000-50000U/L.
Wherein, the concentration of peroxidase is preferably 30000-50000U/L.
Wherein, the concentration of 4-AA is preferably 4-5g/L.
Wherein, the concentration of surfactant is preferably 15-30g/L.
Wherein, 2- (3,5- dimethoxy-4 's-hydroxyphenyl) -4- ethoxyphenyl -5- [4- (dimethylamino) phenyl]-imidazoles
Concentration is preferably 4-5g/L.
Wherein, the pH of A liquid is preferably 8.0-9.0.
Wherein, alcohols solvent is preferably selected from methanol or ethanol.
As another embodiment of this case, wherein, B liquid also includes the chloro-5-trifluoromethylpyridines of 2,3- bis-.
Wherein, the concentration of the chloro-5-trifluoromethylpyridines of 2,3- bis- is preferably 1.5-2.0g/L.
The manufacturing process of this reagent paper is as follows：
Take out after filter paper (whatman filter paper, Hangzhou double-round filter paper etc.) is fully soaked in A liquid, 50-90 DEG C of drying
10-20 minutes；Then soak in B liquid, 30-60 DEG C is dried 10-20 minutes；Then filter paper is pasted on plastic basis material, is cut
Reagent strip is cut into, is saved backup.
The synthesis of 2- (3,5- dimethoxy-4 's-hydroxyphenyl) -4- ethoxyphenyl -5- [4- (dimethylamino) phenyl]-imidazoles
Path is as follows：
This case Test paper colour developing principle is：
Color is done with 2- (3,5- dimethoxy-4 's-hydroxyphenyl) -4- ethoxyphenyl -5- [4- (dimethylamino) phenyl]-imidazoles
Original, in detection, reagent paper color is changed into blue from colourless.And using 2- (3,5- dimethoxy-4 's-hydroxyphenyl) -4- ethoxy benzene
When base -5- [4- (dimethylamino) phenyl]-imidazoles does chromogen, the right height of Linear Quasi of the standard curve done in detection by quantitative
(as shown in Figure 1), this explanation can improve the accuracy of detection to blood uric acid using the chromogen.And according to conventional 2,4,6-
Three bromo- 3- hydroxy benzoic acids make chromogen, change colour first unobvious, although be changed into red from colourless, redness colour developing more chaos mould
Paste, color range difference is little；Also, when configuring standard sample and carrying out standard curve making, the Linear Quasi of the curve of acquisition it is right compared with
Low (R2＜ 0.95).
As this case another program, introduce in B liquid another auxiliary chromogen 2, the chloro-5-trifluoromethylpyridines of 3- bis-,
It can amplify diversity factor of the uric acid concentration to color range, and can improve the linear correlation between serum Uric Acid Concentration and absorbance
Degree, it is concrete as shown in Fig. 2 the right R of Linear Quasi in Fig. 2 so as to further increase the accuracy of detection of reagent paper2=0.9996 outline
Better than the R in Fig. 12=0.9987.
Absorbance in Fig. 1 and Fig. 2 is under 680nm visible light sources, using spectrophotometer or the extinction of hand-held
Degree testing equipment test obtain, by experiment it is found that this reagent paper possess prepare detection serum Uric Acid Concentration ability, it with it is micro-
The combination of type handheld instrument can quickly measure uric acid concentration, so as to the facilitation for realizing detecting.
Although embodiment of the present invention is disclosed as above, it is not restricted to listed in description and embodiment
With, it can be applied to completely various suitable the field of the invention, for those skilled in the art, can be easily
Other modification is realized, therefore under the general concept limited without departing substantially from claim and equivalency range, the present invention is not limited
In specific details.
Claims (10)
1. a kind of blood uric acid Test paper, it is characterised in that by filter paper being immersed successively A liquid, drying, immersion B liquid, being dried again
Drying；Wherein：
The A liquid is included：PBS, urate oxidase, peroxidase, 4-AA and surfactant；
The B liquid is included：2- (3,5- dimethoxy-4 's-hydroxyphenyl) -4- ethoxyphenyl -5- [4- (dimethylamino) phenyl] -
Imidazoles and alcohols solvent.
2. blood uric acid Test paper according to claim 1, it is characterised in that the concentration of the urate oxidase is
30000-50000U/L。
3. blood uric acid Test paper according to claim 1, it is characterised in that the concentration of the peroxidase is
30000-50000U/L。
4. blood uric acid Test paper according to claim 1, it is characterised in that the concentration of the 4-AA is
4-5g/L。
5. blood uric acid Test paper according to claim 1, it is characterised in that the concentration of the surfactant is 15-
30g/L。
6. blood uric acid Test paper according to claim 1, it is characterised in that 2- (3, the 5- dimethoxy-4 's-oxybenzene
Base) -4- ethoxyphenyl -5- [4- (dimethylamino) phenyl]-imidazoles concentration be 4-5g/L.
7. blood uric acid Test paper according to claim 1, it is characterised in that the pH of the A liquid is 8.0-9.0.
8. blood uric acid Test paper according to claim 1, it is characterised in that the alcohols solvent is selected from methanol or second
Alcohol.
9. blood uric acid Test paper according to claim 1, it is characterised in that the B liquid also includes the chloro- 5- of 2,3- bis-
Trifluoromethyl pyridine.
10. blood uric acid Test paper according to claim 9, it is characterised in that the chloro- 5- trifluoromethyls pyrroles of 2, the 3- bis-
The concentration of pyridine is 1.5-2.0g/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611137650.6A CN106596535B (en) | 2016-12-12 | 2016-12-12 | Blood uric acid Test paper |
Applications Claiming Priority (1)
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|---|---|---|---|
| CN201611137650.6A CN106596535B (en) | 2016-12-12 | 2016-12-12 | Blood uric acid Test paper |
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| CN106596535B CN106596535B (en) | 2019-06-25 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108593633A (en) * | 2018-04-19 | 2018-09-28 | 中山大学 | A kind of Test paper for quickly detecting saliva uric acid |
| CN109856131A (en) * | 2019-01-22 | 2019-06-07 | 杭州联晟生物科技有限公司 | It is a kind of for detecting the three-in-one test card and preparation method thereof of uric acid in sample, creatinine, urea simultaneously |
| CN111398268A (en) * | 2020-04-24 | 2020-07-10 | 固安林科特生物工程有限公司 | Peroxidase detection test paper and preparation method and application thereof |
| CN114441516A (en) * | 2021-12-20 | 2022-05-06 | 苏州百源基因技术有限公司 | Uric acid detection kit and preparation method thereof |
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| CN1446925A (en) * | 2003-03-24 | 2003-10-08 | 肖洪武 | Single stable colorimetric reagent of enzyme in liquid state and its application |
| CN101025415A (en) * | 2006-02-23 | 2007-08-29 | 顺德职业技术学院 | Test paper for detecting uric acid content range in urine |
| US20100273249A1 (en) * | 2009-04-24 | 2010-10-28 | Lifescan Scotland Limited | Analytical test strips |
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| CN101025415A (en) * | 2006-02-23 | 2007-08-29 | 顺德职业技术学院 | Test paper for detecting uric acid content range in urine |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108593633A (en) * | 2018-04-19 | 2018-09-28 | 中山大学 | A kind of Test paper for quickly detecting saliva uric acid |
| CN109856131A (en) * | 2019-01-22 | 2019-06-07 | 杭州联晟生物科技有限公司 | It is a kind of for detecting the three-in-one test card and preparation method thereof of uric acid in sample, creatinine, urea simultaneously |
| CN109856131B (en) * | 2019-01-22 | 2022-02-18 | 杭州联晟生物科技有限公司 | Test card for simultaneously detecting uric acid, creatinine and urea in sample and preparation method thereof |
| CN111398268A (en) * | 2020-04-24 | 2020-07-10 | 固安林科特生物工程有限公司 | Peroxidase detection test paper and preparation method and application thereof |
| CN114441516A (en) * | 2021-12-20 | 2022-05-06 | 苏州百源基因技术有限公司 | Uric acid detection kit and preparation method thereof |
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| CN106596535B (en) | 2019-06-25 |
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Effective date of registration: 20190717 Address after: Suzhou City, Jiangsu province 215163 City kolding Road No. 88 Patentee after: SUZHOU INSTITUTE OF BIOMEDICAL ENGINEERING AND TECHNOLOGY, CHINESE ACADEMY OF SCIENCES Address before: Science and Technology City kolding road high tech Zone of Suzhou City, Jiangsu Province, No. 88 215163 Patentee before: Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences |
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Effective date of registration: 20190904 Address after: Room 503, Building 4, 16 Wujinglu, Dongli District Development Zone, Tianjin 300000 Patentee after: Zhongke Jiarui (Tianjin) Medical Science and Technology Development Co., Ltd. Address before: Suzhou City, Jiangsu province 215163 City kolding Road No. 88 Patentee before: SUZHOU INSTITUTE OF BIOMEDICAL ENGINEERING AND TECHNOLOGY, CHINESE ACADEMY OF SCIENCES |
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Application publication date: 20170426 Assignee: TIANJIN GUOKE YIGONG TECHNOLOGY DEVELOPMENT Co.,Ltd. Assignor: Zhongke Jiarui (Tianjin) Medical Science and Technology Development Co.,Ltd. Contract record no.: X2022120000023 Denomination of invention: Blood uric acid test paper Granted publication date: 20190625 License type: Common License Record date: 20220401 |