CN111455020A - 1, 5-sorbitan detection kit and detection method - Google Patents
1, 5-sorbitan detection kit and detection method Download PDFInfo
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- CN111455020A CN111455020A CN202010114208.1A CN202010114208A CN111455020A CN 111455020 A CN111455020 A CN 111455020A CN 202010114208 A CN202010114208 A CN 202010114208A CN 111455020 A CN111455020 A CN 111455020A
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
- C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/30—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving catalase
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
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- C12Q2326/00—Chromogens for determinations of oxidoreductase enzymes
- C12Q2326/90—Developer
- C12Q2326/96—4-Amino-antipyrine
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/902—Oxidoreductases (1.)
- G01N2333/904—Oxidoreductases (1.) acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/902—Oxidoreductases (1.)
- G01N2333/908—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/91—Transferases (2.)
- G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- G01N2333/91205—Phosphotransferases in general
- G01N2333/9121—Phosphotransferases in general with an alcohol group as acceptor (2.7.1), e.g. general tyrosine, serine or threonine kinases
- G01N2333/91215—Phosphotransferases in general with an alcohol group as acceptor (2.7.1), e.g. general tyrosine, serine or threonine kinases with a definite EC number (2.7.1.-)
Abstract
The invention belongs to the technical field of in-vitro diagnosis, and discloses an enzyme method 1, 5-sorbitan detection kit suitable for a full-automatic biochemical analyzer, which is characterized in that the kit adopts a system consisting of hexokinase, pyruvate kinase, glucose-6-phosphate dehydrogenase, lactate dehydrogenase, phosphoenolpyruvate, adenosine triphosphate and β -nicotinamide adenine dinucleotide to eliminate glucose in a sample, and simultaneously adopts a Trinder's reaction system consisting of pyranose oxidase, catalase, N-ethyl-N- (2-hydroxy-3-sulfopropyl) -3-methylaniline sodium salt (TOOS) and 4-antipyrine (4-AA) to quantitatively detect 1, 5-sorbitan in the sample.
Description
Technical Field
The invention belongs to the field of in-vitro diagnosis, and particularly relates to a 1, 5-sorbitan detection kit.
Background
1, 5-anhydrosorbitol (1,5-AG), also known as 1, 5-anhydroglucitol, is a six-carbon monosaccharide having a pyranoid ring structure. The content of 1, 5-sorbitan in human blood and the existing diabetes indexes (such as glucose, glycosylated hemoglobin and glycosylated albumin) show obvious negative correlation, can accurately reflect the average blood sugar level of a patient from several days to a week, and is an important index for diagnosing and confirming the treatment effect of diabetes. In addition, 1,5-AG has other clinical applications, for example, as an indicator of the incidence of postprandial hyperglycemia-related cardiovascular risk in non-diabetic patients; can also well reflect the renal tubule reabsorption function of the chronic renal failure patients, and the like.
The determination of 1, 5-anhydro-sorbitol can be carried out by high performance liquid chromatography, micro-column chromatography, chemiluminescence method, etc. The methods are accurate, but the operation is complicated and the batch detection is difficult, and the methods can only be used in situ and cannot be stored for a long time. The enzyme method determination has the characteristics of no pollution, suitability for a trial and biochemical instrument, simple operation and the like, and is beneficial to large-scale popularization. At present, the pyranose oxidase method is mainly used for clinically measuring 1, 5-anhydrosorbitol, and the principle is that pyranose oxidase (PROD) catalyzes 1, 5-anhydrosorbitol to generate 1, 5-anhydrofructose and hydrogen peroxide, and then a Trinder's reaction system is used for quantitatively measuring the hydrogen peroxide.
However, the pyranose oxidase can simultaneously catalyze glucose and 1, 5-sorbitan in a sample to generate hydrogen peroxide, and the content of the 1, 5-sorbitan in blood is only micromolar, so that the elimination of glucose interference becomes a key step of the detection reagent. The traditional glucose elimination system composed of hexokinase, pyruvate kinase, phosphoenolpyruvate and adenosine triphosphate has limited reaction capability on some hyperglycemia samples within the specified biochemical reaction time, and the 1, 5-sorbitan value of serum is negatively correlated with the blood sugar value, so that the detection result is easy to generate misjudgment on the state of illness of a patient.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention provides a 1, 5-sorbitan detection kit which can realize high-throughput and rapid quantitative detection of human serum 1, 5-sorbitan on a full-automatic biochemical analyzer. The detection kit has the advantages of strong glucose interference resistance, high sensitivity, strong specificity, accurate result and the like, is particularly suitable for a serum sample with hyperglycemia, and is favorable for clinical popularization and use.
The detection kit provided by the invention comprises a detection reagent R1, a detection reagent R2 and a standard substance, and comprises the following specific components:
(1) a reagent R1, which is a solution based on a HEPES buffer system with pH of 6.8-7.5, and contains 10-20 kU/L hexokinase, 5-10 kU/L pyruvate kinase, 5-10 kU/L glucose-6-phosphate dehydrogenase, 5-10 kU/L lactate dehydrogenase, 5-10 mM phosphoenolpyruvate, 1-2 mM adenosine triphosphate and 1-2 mM β -nicotinamide adenine dinucleotide, 1-10 mM N-ethyl-N- (2-hydroxy-3-sulfopropyl) -3-methylaniline sodium salt (TOOS) and a preservative;
(2) a reagent R2, wherein the reagent is a solution based on a HEPES buffer system with the pH of 7.0-8.0, and contains 10-50 kU/L pyranose oxidase, 5-20 kU catalase (HRP), 4-20 mM 4-antipyrine (4-AA) and a preservative;
(3) standard, 1, 5-sorbitan solution, concentration 300 umol/L;
the invention discloses a 1, 5-anhydro-sorbitol detection kit, belonging to a pyranose oxidase method, and the detection principle is as follows: after a sample (standard substance) and the reagent R1 are fully mixed to eliminate glucose, the reagent R2 is added, Trinder's are started to cause the change of the absorbance of the reaction system at the wavelength of 540nm, and then the content of the 1, 5-anhydro-sorbitol in the sample can be calculated by comparing with a standard curve.
The invention discloses a 1, 5-anhydroglucitol detection kit, wherein a glucose elimination system comprises hexokinase, pyruvate kinase, glucose-6-phosphate dehydrogenase, lactate dehydrogenase, phosphoenolpyruvate, adenosine triphosphate and β -nicotinamide adenine dinucleotide, when a sample contains glucose, two crossed enzyme circulation reactions can be formed, reversible reaction catalyzed by hexokinase can be carried out to the utmost in the positive direction, and the purpose of rapidly and completely eliminating glucose is achieved, the circulation reaction process is shown in figure 1, and the figure is abbreviated to correspond to the related substances as HK-hexokinase, PK-pyruvate kinase, G6 PDH-glucose-6-phosphorusAcid dehydrogenase, L DH-lactate dehydrogenase, ATP-adenosine triphosphate, NAD+β -nicotinamide adenine dinucleotide, PA-pyruvic acid, ADP-adenosine diphosphate, NADH-reduced β -nicotinamide adenine dinucleotide, Glu-glucose, G-6-P-glucose-6-phosphate, GA-6-P gluconic acid-6-phosphate.
Drawings
FIG. 1 is a schematic diagram of a cross-cycling reaction.
FIG. 2 is a diagram of the correlation analysis of the kit.
Detailed Description
It should be noted that, the contents described in the following embodiments do not limit the scope of the present invention, and all equivalent flow transformations made by the present specification or directly or indirectly applied to other related technical fields are included in the scope of the present invention.
The first embodiment is as follows: preparation of 1, 5-anhydro-sorbitol detection kit
Preparation of 1, 5-sorbitan assay kit R1:
15kU of hexokinase, 8kU of pyruvate kinase, 10kU of glucose-6-phosphate dehydrogenase, 10kU of lactate dehydrogenase, 10mmol of phosphoenolpyruvate, 1.5 mmol of adenosine triphosphate, 1.5 mmol of β -nicotinamide adenine dinucleotide, 9 mmol of N-ethyl-N- (2-hydroxy-3-sulfopropyl) -3-methylaniline sodium salt (TOOS) and 0.5g of sodium azide are sequentially added into 950ml of 150mM HEPES buffer solution with the pH value of 7.2, the reagent R1 is prepared and stored at 4 ℃.
Preparation of 1, 5-sorbitan assay kit R2:
to 950ml of 150mM HEPES buffer pH 7.2 were added the following in order: 50kU pyranose oxidase, 10kU catalase (HRP), 18 mmol 4-antipyrine (4-AA) and 0.5g sodium azide; stirring evenly, adjusting the pH to 7.2, accurately metering to 1000ml to obtain a reagent R2, and storing at 4 ℃.
Example two: 1, 5-anhydro-sorbitol detection kit, anti-glucose interference test and correlation detection
(1) Setting reaction parameters of a full-automatic biochemical analyzer: biochemical instrument parameters were set according to Table 1
TABLE 1 reaction parameters of fully automatic biochemical analyzer
(2) Obtaining a standard curve
The standard curve was obtained by measuring the concentration of 1, 5-sorbitan at 300. mu. mol/L in the normal saline according to the above measurement procedure.
(3) Anti-glucose interference capability test
A serum sample is taken, the 1, 5-sorbitan and glucose content are measured by using an HP L C method, 50ul of physiological saline containing 100mM glucose is gradually added into the sample, and the 1, 5-sorbitan is detected by using the kit prepared in the first embodiment, and the results are shown in the following table 2.
TABLE 2 glucose interference resistance test
（4）
(5) Correlation detection
Correlation analysis was performed on 50 clinical specimens using hplc and the 1, 5-sorbitan kit of the present invention, and the measured data is shown in fig. 2, and the linear equation obtained was: y = 1.2021x-4.8743, correlation coefficient R2=0.9679, and shows that the accuracy of the detection reagent for detecting 1, 5-dehydrated sorbitol clinical specimens is high.
Claims (2)
1. A1, 5-sorbitan assay kit, comprising:
(1) a reagent R1, which is a solution based on a HEPES buffer system with pH of 6.8-7.5, and contains 10-20 kU/L hexokinase, 5-10 kU/L pyruvate kinase, 5-10 kU/L glucose-6-phosphate dehydrogenase, 5-10 kU/L lactate dehydrogenase, 5-10 mM phosphoenolpyruvate, 1-2 mM adenosine triphosphate and 1-2 mM β -nicotinamide adenine dinucleotide, 1-10 mM 10 mMN-ethyl-N- (2-hydroxy-3-sulfopropyl) -3-methylaniline sodium salt (TOOS) and a preservative;
(2) a reagent R2, wherein the reagent is a solution based on a HEPES buffer system with the pH of 7.0-8.0, and contains 10-50 kU/L pyranose oxidase, 5-20 kU catalase (HRP), 4-20 mM 4-antipyrine (4-AA) and a preservative;
(3) standard, 1, 5-sorbitan solution, at a concentration of 300 umol/L.
2. The method of claim 1, 5-sorbitan assay kit according to claim 1, wherein the assay kit is adapted to a full-automatic biochemical analyzer and comprises a system consisting of hexokinase, pyruvate kinase, glucose-6-phosphate dehydrogenase, lactate dehydrogenase, phosphoenolpyruvate, adenosine triphosphate, and β -nicotinamide adenine dinucleotide to eliminate glucose in the sample, and the Trinder's reaction system consisting of pyranose oxidase, catalase, N-ethyl-N- (2-hydroxy-3-sulfopropyl) -3-methylaniline sodium salt (TOOS), and 4-antipyrine (4-AA) is used to quantitatively assay 1, 5-sorbitan in the sample.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112255219A (en) * | 2020-10-12 | 2021-01-22 | 中拓生物有限公司 | 1, 5-sorbitan determination kit, and preparation method and application thereof |
| CN114134201A (en) * | 2021-11-16 | 2022-03-04 | 苏州普瑞斯生物科技有限公司 | Production process of 1, 5-dehydration-D-sorbitol detection reagent by adopting enzyme method |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06253894A (en) * | 1993-03-03 | 1994-09-13 | Nippon Kayaku Co Ltd | Quantitative determination of 1,5-anhydroglucitol |
| JPH06343492A (en) * | 1993-06-07 | 1994-12-20 | Nippon Kayaku Co Ltd | Method for determining 1,5-anhydroglucitol |
| CN1281980A (en) * | 1999-07-26 | 2001-01-31 | 武汉埃克玛生物技术有限公司 | Serum 1,5-anhydroglucose alcohol determination reagent |
| WO2003071282A1 (en) * | 2002-02-19 | 2003-08-28 | Kyowa Medex Co., Ltd. | Inhibitor of the adhesion of metal oxide-containing material to waste liquor line of automatic analyzer |
| CN102154442A (en) * | 2010-12-30 | 2011-08-17 | 北京九强生物技术股份有限公司 | Method for detecting 1,5-anhydro sorbitol and related diagnostic kit |
| CN102239153A (en) * | 2008-10-07 | 2011-11-09 | 泰博特克药品公司 | New amide compounds as boosters of antivirals |
| CN107703071A (en) * | 2017-09-11 | 2018-02-16 | 三诺生物传感股份有限公司 | 1,5 AG of a kind of detection kit and method |
| CN108034694A (en) * | 2017-12-18 | 2018-05-15 | 广州市进德生物科技有限公司 | A kind of detection kit and its detection method of 1,5- anhydro sorbitols |
-
2020
- 2020-02-25 CN CN202010114208.1A patent/CN111455020A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06253894A (en) * | 1993-03-03 | 1994-09-13 | Nippon Kayaku Co Ltd | Quantitative determination of 1,5-anhydroglucitol |
| JPH06343492A (en) * | 1993-06-07 | 1994-12-20 | Nippon Kayaku Co Ltd | Method for determining 1,5-anhydroglucitol |
| CN1281980A (en) * | 1999-07-26 | 2001-01-31 | 武汉埃克玛生物技术有限公司 | Serum 1,5-anhydroglucose alcohol determination reagent |
| WO2003071282A1 (en) * | 2002-02-19 | 2003-08-28 | Kyowa Medex Co., Ltd. | Inhibitor of the adhesion of metal oxide-containing material to waste liquor line of automatic analyzer |
| CN102239153A (en) * | 2008-10-07 | 2011-11-09 | 泰博特克药品公司 | New amide compounds as boosters of antivirals |
| CN102154442A (en) * | 2010-12-30 | 2011-08-17 | 北京九强生物技术股份有限公司 | Method for detecting 1,5-anhydro sorbitol and related diagnostic kit |
| CN107703071A (en) * | 2017-09-11 | 2018-02-16 | 三诺生物传感股份有限公司 | 1,5 AG of a kind of detection kit and method |
| CN108034694A (en) * | 2017-12-18 | 2018-05-15 | 广州市进德生物科技有限公司 | A kind of detection kit and its detection method of 1,5- anhydro sorbitols |
Non-Patent Citations (2)
| Title |
|---|
| 张颖等: "1,5-脱水山梨醇对糖尿病早期诊断的意义", 《医学综述》 * |
| 简维雄等: "复方钩藤降压片对高血压大鼠左心室肥厚干预的代谢组学研究", 《北京中医药大学学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112255219A (en) * | 2020-10-12 | 2021-01-22 | 中拓生物有限公司 | 1, 5-sorbitan determination kit, and preparation method and application thereof |
| CN114134201A (en) * | 2021-11-16 | 2022-03-04 | 苏州普瑞斯生物科技有限公司 | Production process of 1, 5-dehydration-D-sorbitol detection reagent by adopting enzyme method |
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