CN101025415A - Test paper for detecting uric acid content range in urine - Google Patents
Test paper for detecting uric acid content range in urine Download PDFInfo
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- CN101025415A CN101025415A CN 200610033783 CN200610033783A CN101025415A CN 101025415 A CN101025415 A CN 101025415A CN 200610033783 CN200610033783 CN 200610033783 CN 200610033783 A CN200610033783 A CN 200610033783A CN 101025415 A CN101025415 A CN 101025415A
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Abstract
The invention relates to a test paper strip of using color reaction to detect the concentration of uric acid in urine. It includes test paper base material, its feature is that: enzyme color developing reagent adheres to test paper base material. The beneficial effect of this invention is that, the detector can detect the concentration of uric acid in urine in own home, so as to preliminary reach the range of uric acid content in the urine, thus it is certain guiding significance for the blood uric acid content.
Description
Affiliated technical field
The present invention relates to a kind of test strips of utilizing chromogenic reaction to detect uric acid concentration in the urine, be used for quantitatively measuring fast the urine uric acid level.
Background technology
Uric acid is the oxygenolysis product of purine base, does not have physiological function in human body.The main source of human body purine mainly contains diet, tissue decomposes and from the beginning synthetic three approach.Uric acid is the dead end product of human purine metabolism.Under the normal condition, mainly by renal excretion, account for 2/3, all the other excrete through enteron aisle, skin, hair etc.At present, the blood uric acid is mainly tested on large-scale seralyzer.But the problem that this method exists is: need to use special device, measure that required blood volume is more, the time is longer, cost an arm and a leg, generally can carry out in large hospital.Clinical research shows increasing of uric acid, directly with patients such as gout, hypertension, high fat of blood, diabetes direct relation is arranged, so detects that the content of uric acid has very important significance to clinical diagnosis in the blood.Through clinical testing, the blood uric acid content and the acid content of urinating generally speaking, exist certain correlativity.And the higher number of present stage blood uric acid is remarkable ascendant trend, and how simply, fast, at low cost the problem that therefore detects uric acid concentration is suggested naturally.
Summary of the invention
The objective of the invention is to make things convenient for the test paper of measuring the urine uric acid content fast for patient family and mini clinic provide a kind of, detect by oneself uric acid in patient's urine, concentration to the urine uric acid has certain directive significance, thereby for above-mentioned patient's early diagnosis, and the medication observation of curative effect can provide more economical, easy, safe supervision.
The technical solution adopted for the present invention to solve the technical problems is:
A kind of test paper that detects uric acid content range in the urine comprises the test paper base material, it is characterized in that: be stained with the enzyme developer on the test paper base material.This enzyme developer mainly is made up of uricase and peroxidase, developer.
Wherein enzyme chromogenic reagent composition is composed as follows:
4-amino-antipyrine 4-6mM/L
2-hydroxyl-3,5-dichloro benzosulfonic acid 18-22mM/L
Uricase 0.8-1.2ku/L
Peroxidase 1.88-2.82ku/L
Ferrous hydrofining 0.32-0.48mM/L
Test paper base material bar is immersed in the above-mentioned enzyme chromogenic reagent, take out, low temperature drying.
The invention has the beneficial effects as follows, the first, mainly be that the tester can be in and carries out the detection of urine uric acid concentration voluntarily, reaching the preliminary scope of determining urine uric acid content, thereby blood uric acid content is had certain directive significance, patient's Preliminary detection can be gone to hospital again.And also can play certain supervisory function bit to patient's medication process; The second, can not use special detecting instrument; Three, can save testing cost greatly.The detection paper only use takes the expense less than a yuan.
Description of drawings
Fig. 1 is the test paper synoptic diagram.
Fig. 2 is the colorimetric card synoptic diagram.
Wherein figure (1) is a test strips, and the front portion is divided into the reagent part; Figure (2) colorimetric card (annotate: color be shallower than No. 2 for normal; Color between No. 2 with No. 3 be undesired; Color is deeper than No. 3 the severe overweight that is.)
Embodiment
The present invention is further described below in conjunction with drawings and Examples.
A kind of test paper that detects uric acid content range in the urine comprises test paper base material 10, it is characterized in that: be stained with enzyme developer 20 on the test paper base material.This enzyme developer 20 mainly is made up of uricase and peroxidase, developer.
Wherein enzyme chromogenic reagent composition is composed as follows:
4-amino-antipyrine 4-6mM/L
2-hydroxyl-3,5-dichloro benzosulfonic acid 18-22mM/L
Uricase 0.8-1.2ku/L
Peroxidase 1.88-2.82ku/L
Ferrous hydrofining 0.32-0.48mM/L
Test paper base material bar 10 is immersed in the enzyme chromogenic reagent 20, take out, low temperature drying.
The reagent that adheres on this test strips can generate a kind of red elder brother's imines material with uric acid, and the growing amount of pigment is directly proportional with the concentration of uric acid in the sample.During detection, with the plastic cup urine that takes a morsel, test strips is immersed about 2 seconds in the urine, take out, normal temperature is observed color after following 5 minutes, and with standard color card 30 relatively, can determine the scope of uric acid content in the urine, thereby blood uric acid content is had the certain guidance meaning.
More convenient when patient uses, normal patient is drained in the acid of urinating for major part, or the people who does not suffer from special disease, can use, and in addition, the patient during also can being used for the treatment of observes result of treatment.In clinical practice, blood uric acid is more convenient than detecting in the application of this test paper, safety, economy.And the present stage patient that raises of blood uric acid is obvious ascendant trend, and the invention of this test paper will make the patient of a myriad of be benefited.
Above-described specific embodiment only is preferred embodiment of the present invention, and the equivalent arrangements of being done according to the present patent application claim all should be technology of the present invention and contains such as.
Claims (3)
1. a test paper that detects uric acid content range in the urine comprises the test paper base material, it is characterized in that: be stained with the enzyme developer on the test paper base material.
2. a kind of test paper that detects uric acid content range in the urine according to claim 1 is characterized in that: enzyme chromogenic reagent principal ingredient is: uricase and peroxidase, developer.
3. a kind of test paper that detects uric acid content range in the urine according to claim 2 is characterized in that enzyme chromogenic reagent constituent concentration is composed as follows:
4-amino-antipyrine 4-6mM/L
2-hydroxyl-3,5-dichloro benzosulfonic acid 18-22mM/L
Uricase 0.8-1.2ku/L
Peroxidase 1.88-2.82ku/L
Ferrous hydrofining 0.32-0.48mM/L
Phosphate buffer 1 .2-1.8M/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610033783 CN101025415A (en) | 2006-02-23 | 2006-02-23 | Test paper for detecting uric acid content range in urine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610033783 CN101025415A (en) | 2006-02-23 | 2006-02-23 | Test paper for detecting uric acid content range in urine |
Publications (1)
| Publication Number | Publication Date |
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| CN101025415A true CN101025415A (en) | 2007-08-29 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 200610033783 Pending CN101025415A (en) | 2006-02-23 | 2006-02-23 | Test paper for detecting uric acid content range in urine |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102608113A (en) * | 2012-03-30 | 2012-07-25 | 上海三爱思试剂有限公司 | Mildew-proof pH test paper and preparation method thereof |
| CN102998443A (en) * | 2011-09-14 | 2013-03-27 | 佳木斯大学 | Immune colloidal gold test strip for detecting uric acid in urine, and its manufacturing method |
| CN102998439A (en) * | 2011-09-14 | 2013-03-27 | 佳木斯大学 | Micro-fluidic paper chip for simultaneously detecting glucose, uric acid, triglycerides and cholesterols, and its manufacturing method |
| CN104749174A (en) * | 2014-12-18 | 2015-07-01 | 东莞星显家具有限公司 | Urine tyrosine detecting method |
| CN106290323A (en) * | 2015-06-04 | 2017-01-04 | 章丘美高义医疗器械有限公司 | A kind of stable, uric acid reagent that capacity of resisting disturbance is strong and detection method |
| CN106404758A (en) * | 2015-07-28 | 2017-02-15 | 珠海和凡医药股份有限公司 | Test paper for detecting uric acid content range in urine |
| CN106596535A (en) * | 2016-12-12 | 2017-04-26 | 中国科学院苏州生物医学工程技术研究所 | Blood uric acid detection test paper |
| CN106645128A (en) * | 2017-01-03 | 2017-05-10 | 长沙中生众捷生物技术有限公司 | Detection reagent and test paper for uric acid |
| CN108593633A (en) * | 2018-04-19 | 2018-09-28 | 中山大学 | A kind of Test paper for quickly detecting saliva uric acid |
| CN108872560A (en) * | 2018-08-14 | 2018-11-23 | 湖北伽诺美生物科技有限公司 | A kind of portable urine quantitative testing device |
| CN109916891A (en) * | 2019-04-12 | 2019-06-21 | 吉林省汇酉生物技术股份有限公司 | A kind of dry chemistry reagent piece and preparation method thereof quantitative determining uric acid concentration |
| CN111077143A (en) * | 2019-12-24 | 2020-04-28 | 杭州启棣生物技术有限公司 | Uric acid detection method and device |
-
2006
- 2006-02-23 CN CN 200610033783 patent/CN101025415A/en active Pending
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102998443A (en) * | 2011-09-14 | 2013-03-27 | 佳木斯大学 | Immune colloidal gold test strip for detecting uric acid in urine, and its manufacturing method |
| CN102998439A (en) * | 2011-09-14 | 2013-03-27 | 佳木斯大学 | Micro-fluidic paper chip for simultaneously detecting glucose, uric acid, triglycerides and cholesterols, and its manufacturing method |
| CN102608113A (en) * | 2012-03-30 | 2012-07-25 | 上海三爱思试剂有限公司 | Mildew-proof pH test paper and preparation method thereof |
| CN102608113B (en) * | 2012-03-30 | 2014-08-13 | 上海三爱思试剂有限公司 | Mildew-proof pH test paper and preparation method thereof |
| CN104749174A (en) * | 2014-12-18 | 2015-07-01 | 东莞星显家具有限公司 | Urine tyrosine detecting method |
| CN106290323A (en) * | 2015-06-04 | 2017-01-04 | 章丘美高义医疗器械有限公司 | A kind of stable, uric acid reagent that capacity of resisting disturbance is strong and detection method |
| CN106404758A (en) * | 2015-07-28 | 2017-02-15 | 珠海和凡医药股份有限公司 | Test paper for detecting uric acid content range in urine |
| CN106596535A (en) * | 2016-12-12 | 2017-04-26 | 中国科学院苏州生物医学工程技术研究所 | Blood uric acid detection test paper |
| CN106596535B (en) * | 2016-12-12 | 2019-06-25 | 中国科学院苏州生物医学工程技术研究所 | Blood uric acid Test paper |
| CN106645128A (en) * | 2017-01-03 | 2017-05-10 | 长沙中生众捷生物技术有限公司 | Detection reagent and test paper for uric acid |
| CN108593633A (en) * | 2018-04-19 | 2018-09-28 | 中山大学 | A kind of Test paper for quickly detecting saliva uric acid |
| CN108872560A (en) * | 2018-08-14 | 2018-11-23 | 湖北伽诺美生物科技有限公司 | A kind of portable urine quantitative testing device |
| CN109916891A (en) * | 2019-04-12 | 2019-06-21 | 吉林省汇酉生物技术股份有限公司 | A kind of dry chemistry reagent piece and preparation method thereof quantitative determining uric acid concentration |
| CN111077143A (en) * | 2019-12-24 | 2020-04-28 | 杭州启棣生物技术有限公司 | Uric acid detection method and device |
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Open date: 20070829 |