CN100570339C - Measure the reagent and the method for quick thereof of zinc content in the urine - Google Patents
Measure the reagent and the method for quick thereof of zinc content in the urine Download PDFInfo
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- CN100570339C CN100570339C CNB2005100485215A CN200510048521A CN100570339C CN 100570339 C CN100570339 C CN 100570339C CN B2005100485215 A CNB2005100485215 A CN B2005100485215A CN 200510048521 A CN200510048521 A CN 200510048521A CN 100570339 C CN100570339 C CN 100570339C
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Abstract
The present invention is a kind of reagent and the method for quick thereof of measuring zinc content in the urine that provides for the detection that solves zinc content in the urine, its reagent is by strong acid, sodium thiocyanate solution and crystal violet solution are formed, its method for rapidly testing is to add tested urine 0.5ml in test tube, add strong acid successively, sodium thiocyanate solution and crystal violet solution, mixing, observations in 2 minutes, reagent of the present invention is easy to use, the result is accurate, intuitively, simple to operate quick, be suitable for hospitals at different levels, health care institute instructs the correct zinc supplementation of various crowds, prevents to lack zinc and zinc poisoning, very easily penetration and promotion is used, and makes and benefits the healthy of people.
Description
One, technical field
A kind of reagent and method for quick thereof of measuring zinc content in the urine that the present invention relates to medically use.
Two, background technology
Zinc is one of trace element of wanting of body weight for humans, is only second to iron at the content of human body.Children lack zinc and can slow down and grow, because zinc participates in the synthetic of human growth hormone, influential to protein metabolism and fat absorption, next is that the sense of taste is paused late, anorexia, scarce zinc make phosphatase minimizing in the saliva, so the taste bud hypofunction, while is owing to food intake reduces, even the generation canker sore; Also affect the nerves system's internal protein and nucleic acid of scarce zinc is synthetic, thereby children's's IQ is descended, and produces symptoms such as lassitude, depression, and trembling and abnormal behavior also appears in the children's who has; Diarrhoea, skin lesion, the difficult healing of wound etc. also can be seen; Infant of low-birth weight's blood zinc is crossed to hang down and also can be produced edema, Hypoproteinemia.In addition, scarce zinc makes children's's immune system influenced, and cellular immunity and humoral immune function are unusual, and resistibility descends, thereby easily causes infection, particularly suffers from respiratory tract infection repeatedly.The scarce zinc of pregnant woman can cause slow, the lopsided or miscarriage of fetal growth, occupy the first by its neonate's neural tube defects that causes in various neonate's inborn defects, and the scarce zinc of man at reproduction age can cause sterility.
But zinc is too high in the body, and the content of interior vitamin C of body and iron is reduced, and suppresses the absorption and the utilization of iron, thereby causes hypoferric anemia; The ratio of zinc, copper in the body is increased, influence the metabolism of cholesterol, blood fat is raise, it is atherosis to impel artery to take place, and hides some dangers for for get cardiovascular and cerebrovascular diseases in the future.In addition, zinc too high levels in the body also has the danger of bringing out cancer.
Therefore, the situation of zinc in the regular human body, can correctly judge zinc metaboilic level, particularly children, especially school-ager in the body, be in the brain growth critical period, in time understand the zinc metaboilic level, suitably replenish zinc, prevent the shortage of zinc, brain growth there is very big effect, significant meaning is arranged improving crowd's quality.
The detection of zinc has the zinc of sending out, blood zinc and three kinds of methods of urine zinc.Send out the atomic absorption spectrophotometer that zinc detects needs costliness, and hair is subject to environmental pollution, influence testing result, when human body lacks zinc, influence protein synthesis, hair growth is slowed down, zinc-emitting content increases on the contrary, also influence the result, blood zinc detects and belongs to traumatic detection, might infect blood borne disease, and blood zinc content is subject to various physiology (as meals, gestation etc.) pathology (as stress, liver and kidney disease etc.) influence, when lacking zinc, organize human body capacity of decomposition to strengthen, intracellular zinc discharges into circulation, and blood zinc content increases on the contrary, also influences the result, testing result needs to be difficult for early detection in conjunction with clinical symptoms.When human body lacked zinc, the row that body can protectiveness ground reduces zinc was rushed down to keep the balance of zinc in the body, therefore urinate zinc detect can the early detection body in the dynamic change of zinc, accurately corresponding zinc metaboilic level in the antimer.But the detection formality of urinating zinc at present is loaded down with trivial details, the cost height, and how therefore unanimity, measured zinc content in the urine fast and accurately in urinating the zinc test sometimes, and it is very necessary being beneficial to the healthy of people.
Three, summary of the invention
As everybody knows, reagent is a kind of detection articles for use commonly used in the medical science, by the reagent difference variation of color in some cases, contrast and draw the content of certain composition, the present invention is based upon under these actual conditions just, a kind of reagent and the method for quick thereof of measuring zinc content in the urine that provides for the detection that solves zinc content in the urine, its reagent is the strong acid 0.2ml by 0.1M-1.0M, mass concentration is that the sodium thiocyanate solution 0.05ml of 1-5% and crystal violet solution 0.05ml that mass concentration is 0.05-1% form, its method for rapidly testing is to add tested urine 0.5ml in test tube, the strong acid 0.2ml that adds 0.1-1.0M, mixing, adding mass concentration again is the sodium thiocyanate solution 0.05ml of 1-5%, fully add the crystal violet solution 0.05ml that mass concentration is 0.05-1.0% again behind the mixing, observations in 2 minutes, reagent of the present invention is easy to use, the result is accurate, intuitively, simple to operate quick, do not need any special equipment, be suitable for hospitals at different levels, health care institute (institute), instruct the correct zinc supplementation of various crowds, prevent to lack zinc and zinc poisoning, outstanding clinical use value is arranged, very easily penetration and promotion is used, and makes and benefits the healthy of people.
Four, embodiment
Below in conjunction with concrete condition and embodiment the specific embodiment of the present invention is elaborated.
Provided by technique scheme, reagent of the present invention is made up of the crystal violet solution 0.05ml that the strong acid 0.2ml of 0.1-1.0M, sodium thiocyanate solution 0.05ml that mass concentration is 1-5% and mass concentration are 0.05-1%.
Said strong acid can adopt hydrochloric acid or sulfuric acid; Crystal violet is a kind of commercially available dyestuff chemically.
Embodiment 1
Reagent of the present invention is that 1% sodium thiocyanate solution 0.05ml and mass concentration are that 0.05% crystal violet solution 0.05ml forms by sulfuric acid 0.2ml, the mass concentration of 0.1M;
Embodiment 2
Reagent of the present invention also can be made up of the hydrochloric acid 0.2ml of following dose of liquid: 0.65M, 1.2% sodium thiocyanate solution 0.05ml and 0.1% crystal violet solution 0.05ml;
Embodiment 3
Reagent of the present invention also can be made up of the hydrochloric acid 0.2ml of following dose of liquid: 1.0M, 5% sodium thiocyanate solution 0.05ml and 1% crystal violet solution 0.05ml.For convenience, the strong acid of 0.1-1.0M is called liquid No. 1 in the detection, and the sodium thiocyanate solution of 1-5% is called liquid No. 2, and the crystal violet solution of 0.05-1.0% is called liquid No. 3.
Use reagent of the present invention to measure that the zinc content method for quick is in the urine:
In test tube, add the detected urine of 0.5ml, strong acid (hydrochloric acid or the sulfuric acid all can) 0.2ml that adds 0.1-1.0M again, mixing, afterwards, adding mass concentration is the sodium thiocyanate solution 0.05ml of 1-5%, fully add the crystal violet solution 0.05ml mixing that mass concentration is 0.05-1.0% again behind the mixing, observations in 2 minutes, the result after 2 minutes is invalid, when urinating zinc content≤15 μ g/dl in 2 minutes, it is green that reagent is, the urine zinc content is that content is normal greater than 15 μ g/dl during less than 120 μ g/dl, and reagent gradually becomes blue by green, during urine zinc content 〉=120 μ g/dl, reagent is bluish violet.
The present invention has all obtained satisfied effect through repeatedly on probation repeatedly, and compares with additive method, and accuracy rate is up to 97.22%, and is existing with the clinical comparison method and test the urine examination situation of 36 examples details are as follows:
Method is, get 17 detector tubes, adding in pipe respectively and containing zinc is 0 μ g/dl, 10 μ g/dl, 15 μ g/dl, 20 μ g/dl, 30 μ g/dl, 40 μ g/dl, 50 μ g/dl, 60 μ g/dl, 70 μ g/dl, 80 μ g/dl, 90 μ g/dl, 100 μ g/dl, 110 μ g/dl, 120 μ g/dl, 130 μ g/dl, the zinc titer 0.5ml of 140 μ g/dl and 150 μ g/dl, in every pipe, add 0.65M hydrochloric acid 0.2ml mixing, add 1.2% sodium thiocyanate solution 0.05ml again, abundant mixing, afterwards, each Guan Zhongzai adds 0.1% crystal violet solution 0.05ml respectively, mixing, observations in 2 minutes, the result is invalid after 2 minutes, and during urine zinc content≤15 μ g/dl, it is green that reagent is, the urine zinc content is greater than 15 μ g/dl, during less than 120 μ g/dl, the urine zinc content is normal, and reagent colour development gradually becomes blue by green, during urine zinc content 〉=120 μ g/dl, reagent is bluish violet.The test comparison situation such as the following table of 36 examples:
By as can be seen above-mentioned, the present invention removes the urine zinc content in the detection of 36 examples have unusual (unknown origin) when 25 μ g/dl, other detection requirements all according to the invention, accuracy rate is 97.22%, and unusual (unknown origin), other detection requirements all according to the invention, accuracy rate is 97.22%, and compares with additive method, pyridine-azo maphthol (PAN) colourimetry in as above showing, need to use the prussiate violent in toxicity, use dangerously, and need spectrophotometer, complex operation, the inventive method does not contain hypertoxic prussiate, and is safe and reliable, without any need for special instrument.
In a word, the invention provides the reagent and the rapid assay methods thereof of zinc content in the urine of zinc metaboilic level in the accurate antimer of a kind of energy, be applicable to the external test of urine zinc content,, prevent to lack zinc and zinc poisoning to instruct the correct zinc supplementation of various crowds.Its result is accurate and visual, and is simple to operate quick, do not need any equipment, is suitable for hospitals at different levels, health care institute's (institute) routine inspection and healthy primary dcreening operation etc.Its novelty of the present invention is apparent, and penetration and promotion is used and must be brought benefit to the mankind.
Claims (7)
1, a kind of reagent of measuring zinc content in the urine is characterized in that, this reagent is made up of the crystal violet solution 0.05ml that the strong acid 0.2ml of 0.1-1.0M, sodium thiocyanate solution 0.05ml that mass concentration is 1-5% and mass concentration are 0.05-1%.
2, the reagent of zinc content in the measurement urine according to claim 1 is characterized in that said strong acid is hydrochloric acid or sulfuric acid.
3, the reagent of zinc content in the measurement urine according to claim 1, it is characterized in that said reagent is exactly to be that 1% sodium thiocyanate solution 0.05ml and mass concentration are that 0.05% crystal violet solution 0.05ml forms by the sulfuric acid 0.2ml of 0.1M, mass concentration.
4, the reagent of zinc content in the measurement urine according to claim 1 is characterized in that, said reagent is made up of the hydrochloric acid 0.2ml of 0.65M, 1.2% sodium thiocyanate solution 0.05ml and 0.1% crystal violet solution 0.05ml.
5, the reagent of zinc content in the measurement urine according to claim 1 is characterized in that, said reagent is made up of the hydrochloric acid 0.2ml of 1.0M, 5% sodium thiocyanate solution 0.05ml and 1% crystal violet solution 0.05ml.
6, adopt the described reagent method for quick of claim 1, it is characterized in that, in test tube, add the detected urine of 0.5ml, the strong acid 0.2ml that adds 0.1-1.0M again, mixing, afterwards, adding mass concentration is the sodium thiocyanate solution 0.05ml of 1-5%, fully add the crystal violet solution 0.05ml mixing that mass concentration is 0.05-1.0% again behind the mixing, observations in 2 minutes, the result after 2 minutes is invalid, when urinating zinc content≤15 μ g/dl in 2 minutes, it is green that reagent is, the urine zinc content is that content is normal greater than 15 μ g/dl during less than 120 μ g/dl, and reagent gradually becomes blue by green, during urine zinc content 〉=120 μ g/dl, reagent is bluish violet.
7, method according to claim 6, it is characterized in that, in test tube, respectively add urine 0.5ml, add 0.65M hydrochloric acid 0.2ml mixing, add 1.2% sodium thiocyanate solution 0.05ml again, abundant mixing, afterwards, each Guan Zhongzai adds 0.1% crystal violet solution 0.05ml, mixing respectively, observations in 2 minutes, the result is invalid after 2 minutes, and during urine zinc content≤15 μ g/dl, it is green that reagent is, the urine zinc content is greater than 15 μ g/dl, during less than 120 μ g/dl, the urine zinc content is normal, and reagent colour development gradually becomes blue by green, during urine zinc content 〉=120 μ g/dl, reagent is bluish violet.
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| CNB2005100485215A CN100570339C (en) | 2005-11-14 | 2005-11-14 | Measure the reagent and the method for quick thereof of zinc content in the urine |
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| CNB2005100485215A CN100570339C (en) | 2005-11-14 | 2005-11-14 | Measure the reagent and the method for quick thereof of zinc content in the urine |
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| CN106093026A (en) * | 2016-07-06 | 2016-11-09 | 哈尔滨贝贝凯尔科技发展有限公司 | There is child's diaper and the method for detecting microelements of urine detection function |
| CN106198523A (en) * | 2016-07-06 | 2016-12-07 | 哈尔滨贝贝凯尔科技发展有限公司 | Trace element detection reagent paper and preparation method thereof |
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Non-Patent Citations (6)
| Title |
|---|
| 微量锌测定方法研究及应用. 罗大莉.四川化工与腐蚀控制，第2卷第4期. 1999 |
| 微量锌测定方法研究及应用. 罗大莉.四川化工与腐蚀控制，第2卷第4期. 1999 * |
| 硫氰酸盐结晶紫光度法测定痕量锌（Ⅱ）. 吴瑞巧.理化检验-化学分册，第41卷第3期. 2005 |
| 硫氰酸盐结晶紫光度法测定痕量锌（Ⅱ）. 吴瑞巧.理化检验-化学分册，第41卷第3期. 2005 * |
| 镉基体中微量锌的富集与分离研究. 蔡震峰.广州化学，第30卷第1期. 2005 |
| 镉基体中微量锌的富集与分离研究. 蔡震峰.广州化学，第30卷第1期. 2005 * |
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