CN218824309U - Influenza A and B virus and novel coronavirus double-channel saliva detection card - Google Patents
Influenza A and B virus and novel coronavirus double-channel saliva detection card Download PDFInfo
- Publication number
- CN218824309U CN218824309U CN202222145897.XU CN202222145897U CN218824309U CN 218824309 U CN218824309 U CN 218824309U CN 202222145897 U CN202222145897 U CN 202222145897U CN 218824309 U CN218824309 U CN 218824309U
- Authority
- CN
- China
- Prior art keywords
- influenza
- pad
- card
- colloidal gold
- virus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The utility model discloses a influenza A, B virus and novel coronavirus binary channels saliva detection card, including plastic card and saliva suction head, the inside of plastic card is equipped with detection card passageway one and detection card passageway two, saliva suction head locates plastic card one end and with detection card passageway one and detection card passageway two intercommunication, detection card passageway one includes sample pad, colloidal gold pad, nitrocellulose reaction membrane, water absorption pad; detect card channel two and include sample pad two, colloidal gold pad two, nitrocellulose reaction membrane two, absorb water and fill up two, the utility model discloses following beneficial effect has: can distinguish influenza A virus, type B virus and novel coronavirus respectively by the specificity simultaneously, adopt the binary channels design, sensitivity and specificity are higher to saliva is as detecting the sample, and the sample easily obtains, need not swab sampling, can simplify influenza A virus, type B virus and novel coronavirus's joint detection flow, easy operation, convenience, detection cycle are short.
Description
Technical Field
The utility model relates to a virus short-term test technical field specifically indicates a influenza A, type B virus and novel coronavirus binary channels saliva detects card.
Background
Influenza is an acute respiratory infectious disease caused by influenza virus infection, is rapidly transmitted through respiratory tracts, has short latency and obvious seasonality, is very easy to cause large-scale outbreak and has high control difficulty. Influenza viruses are classified into influenza A, influenza B and influenza C3 types, and human influenza A and influenza B viruses mainly cause serious threats to human health. Influenza a is clinically manifested as fever, headache, myalgia and general malaise, and may be accompanied by respiratory system symptoms such as cough, expectoration, etc., and may also be accompanied by digestive system symptoms such as vomiting, diarrhea, etc.
Because the symptoms of the human body infected by the novel coronavirus are similar to those caused by the influenza A and B viruses, the influenza A and B viruses and the novel coronavirus are difficult to distinguish from the symptoms such as fever, and the combined infection is common in respiratory diseases. If fever cases can not be diagnosed quickly and accurately in time, influenza A and B cases are possibly treated by being infected by the same novel coronavirus, the number of the combined infections of patients is large, the hidden danger of cross infection is large, and the prevention and the control of the novel coronavirus are extremely adverse effects.
Most of the existing rapid detection products in the current market can only carry out single detection of new coronary diseases or influenza, and are swab samples, sampling needs special swabs, sampling personnel needs special training, application of the sampling personnel is greatly limited, saliva samples do not need special tools due to easy obtaining of the saliva samples, and the rapid detection method has many advantages. Therefore, the development of a dual-channel detection card for rapidly and jointly detecting the infection of the influenza A virus, the influenza B virus and the novel coronavirus in saliva is of great significance.
SUMMERY OF THE UTILITY MODEL
The utility model provides a dual-channel saliva detection card for influenza A virus, influenza B virus and novel coronavirus to solve the above problems.
In order to solve the technical problem, the utility model adopts the technical scheme that: a dual-channel saliva detection card for influenza A and B viruses and novel coronaviruses comprises a plastic card and a saliva suction head, wherein a first detection card channel and a second detection card channel are arranged in the plastic card, the saliva suction head is arranged at one end of the plastic card and is communicated with the first detection card channel and the second detection card channel, and the first detection card channel comprises a sample pad, a colloidal gold pad, a nitrocellulose reaction membrane and a water absorption pad; the second detection card channel comprises a second sample pad, a second colloidal gold pad, a second nitrocellulose reaction membrane and a second water absorption pad;
sample pad, colloidal gold pad, cellulose nitrate reaction membrane and absorb water and fill up to paste in proper order constitute detection channel one on the plastics card, sample pad two, colloidal gold pad two, cellulose nitrate reaction membrane two and absorb water and fill up two and paste in proper order constitute detection channel two on the plastics card, the colloidal gold pad is for containing the colloidal gold sign influenza A virus antibody one and the colloidal gold pad of influenza B virus antibody one, the colloidal gold pad two is for containing the colloidal gold sign novel coronavirus antibody one colloidal gold pad.
Further, the nitrocellulose reaction membrane is provided with a detection line T1, a detection line T2 and a quality control line C1, the nitrocellulose reaction membrane II is provided with a detection line T3 and a quality control line C2, the detection line T1 and the detection line T2 are both located on one side, close to the colloidal gold pad, of the nitrocellulose reaction membrane II, the detection line T3 is located on one side, close to the colloidal gold pad II, of the nitrocellulose reaction membrane II, the detection line T1 is coated with a second influenza A virus antibody, the detection line T2 is coated with a second influenza B virus antibody, the detection line T3 is coated with a second novel coronavirus antibody, the quality control line C1 is located on one side, away from the colloidal gold pad, of the nitrocellulose reaction membrane II, the quality control line C2 is located on one side, away from the colloidal gold pad II, and the quality control line C1 and the quality control line C2 contain an anti-goat IgG polyclonal antibody.
Further, the first influenza A virus antibody and the second influenza A virus antibody are antibodies with different epitopes, the first influenza B virus antibody and the second influenza B virus antibody are antibodies with different epitopes, the first novel coronavirus antibody and the second novel coronavirus antibody are antibodies with different epitopes, and the antibodies are artificially prepared antibodies.
Further, the detection card takes saliva as a detection sample.
The utility model discloses following beneficial effect has: can distinguish influenza A virus, type B virus and novel coronavirus respectively by the specificity simultaneously, adopt the binary channels design, sensitivity and specificity are higher to saliva is as detecting the sample, and the sample easily obtains, need not swab sampling, can simplify influenza A virus, type B virus and novel coronavirus's joint detection flow, easy operation, convenience, detection cycle are short.
Drawings
FIG. 1 is a front view of a dual channel saliva test card for influenza A and B viruses and novel coronaviruses of the present invention;
FIG. 2 is a schematic diagram showing the positive test results of the dual-channel saliva test card for influenza A virus, influenza B virus and novel coronavirus of the present invention, wherein (1) the test results are positive for influenza A virus and new corona virus, (2) the test results are negative for influenza A virus and new corona virus, and (3) the test results are positive for influenza A virus and new corona virus;
FIG. 3 is a schematic diagram showing negative results of the dual-channel saliva test card for influenza A and B viruses and novel coronavirus of the present invention;
fig. 4 is a schematic diagram showing that the detection result of the dual-channel saliva detection card for influenza a virus, influenza b virus and novel coronavirus of the present invention is invalid.
As shown in the figure: 1. a saliva pipette tip; 2. a sample pad; 3. a colloidal gold pad; 4. a cellulose nitrate reaction membrane; 5. a water absorbent pad; 6. a water absorbent pad; 7. a second sample pad; 8. a second colloidal gold pad; 9. a second cellulose nitrate reaction membrane; 10. and a second water absorption pad.
Detailed Description
The invention is further illustrated by the following specific examples, which are intended to be illustrative and not limiting, and to illustrate and explain the invention.
Example 1
A dual-channel saliva detection card for influenza A and B viruses and novel coronaviruses comprises a plastic card 6 and a saliva suction head 1, wherein a first detection card channel and a second detection card channel are arranged in the plastic card 6, the saliva suction head 1 is arranged at one end of the plastic card 6 and is communicated with the first detection card channel and the second detection card channel, and the first detection card channel comprises a sample pad 2, a colloidal gold pad 3, a nitrocellulose reaction membrane 4 and a water absorption pad 5; the second detection card channel comprises a second sample pad 7, a second colloidal gold pad 8, a second nitrocellulose reaction membrane 9 and a second water absorption pad 10;
in this embodiment, sample pad 2, colloidal gold pad 3, nitrocellulose reaction membrane 4 and water absorption pad 5 paste in proper order constitute detection channel one on the plastic card 6, sample pad two 7, colloidal gold pad two 8, nitrocellulose reaction membrane two 9 and water absorption pad two 10 paste in proper order constitute detection channel two on the plastic card 6, colloidal gold pad 3 is the colloidal gold pad that contains colloidal gold labeled influenza a virus antibody one and influenza b virus antibody one, colloidal gold pad two 8 is the colloidal gold pad that contains colloidal gold labeled novel coronavirus antibody one.
In this embodiment, the nitrocellulose reaction membrane 4 is provided with a detection line T1, a detection line T2 and a quality control line C1, the nitrocellulose reaction membrane two 9 is provided with a detection line T3 and a quality control line C2, the detection line T1 and the detection line T2 are both located on one side of the nitrocellulose reaction membrane 4 close to the colloidal gold pad 3, the detection line T3 is located on one side of the nitrocellulose reaction membrane two 9 close to the colloidal gold pad two 8, the detection line T1 is coated with a influenza a virus antibody two, the detection line T2 is coated with a influenza b virus antibody two, the detection line T3 is coated with a novel coronavirus antibody two, the quality control line C1 is located on one side of the nitrocellulose reaction membrane 4 away from the colloidal gold pad 3, the quality control line C2 is located on one side of the nitrocellulose reaction membrane two 9 away from the colloidal gold pad two 8, and the quality control lines C1 and C2 contain an anti-goat IgG polyclonal antibody.
In this embodiment, the first influenza a virus antibody and the second influenza a virus antibody are antibodies with different epitopes, the first influenza b virus antibody and the second influenza b virus antibody are antibodies with different epitopes, the first novel coronavirus antibody and the second novel coronavirus antibody are also antibodies with different epitopes, and the antibodies are artificially prepared antibodies.
Mode of use
The utility model also provides a use of influenza A, B type virus and novel coronavirus binary channels saliva detects card, specifically as follows:
1) Collecting a sufficient amount of saliva sample;
2) Contacting the saliva pipette tip 1 with the saliva sample for at least 2 minutes, where the saliva pipette tip of the test card can be dipped into the saliva sample, whereupon the saliva sample will move in sequence along the respective attachments on the plastic card in the direction of the absorbent pad;
3) When liquid appears in the observation window, the saliva sample is placed on a clean and flat workbench, and the detection results of the influenza A virus, the influenza B virus and the novel coronavirus in the saliva sample are obtained after 15 minutes.
If the saliva sample contains influenza A, influenza B virus or novel coronavirus or a plurality of viruses, the saliva sample is specifically combined with the colloidal gold marked influenza A and influenza B virus antibodies or novel coronavirus antibodies on the detection card to form a complex, then the complex moves forward, and is specifically combined with the influenza A and influenza B virus antibodies or the novel coronavirus antibodies in the process of passing through the nitrocellulose membrane, and the detection line T1, T2 or T3 shows red according to the corresponding appearance of the detected viruses, so that the sample to be detected is positive, and the reference is made to FIG. 2.
If the saliva sample does not contain influenza A virus, influenza B virus or novel coronavirus, the detection lines T1, T2 and T3 do not appear red, and the sample is negative; the colloidal gold labeled influenza A and B virus antibodies and the novel coronavirus antibody continue to advance to be combined with the goat anti-mouse IgG polyclonal antibody, and red colors appear at the positions of quality control lines C1 and C2, and refer to FIG. 3;
whether the sample contains influenza A virus and influenza B virus or novel coronavirus, the influenza A virus antibody and the influenza B virus antibody marked by the colloidal gold and the novel coronavirus antibody respectively continue to move to the quality control lines C1 and C2 respectively, and are combined with the goat anti-mouse IgG polyclonal antibody coated at the two positions to form red bands, namely the quality control lines. If the quality control line does not appear in the detection process, the detection card is proved to be invalid or the operation is failed, the result is invalid, and the detection needs to be carried out again, and the reference is made to fig. 4.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of modifications can be made without departing from the principles of the present invention, and these modifications should also be regarded as the protection scope of the present invention.
Claims (4)
1. A dual-channel saliva detection card for influenza A and B viruses and novel coronaviruses comprises a plastic card (6) and a saliva liquid suction head (1), wherein a first detection card channel and a second detection card channel are arranged inside the plastic card (6), and the saliva liquid suction head (1) is arranged at one end of the plastic card (6) and is communicated with the first detection card channel and the second detection card channel, and comprises a sample pad (2), a colloidal gold pad (3), a nitrocellulose reaction membrane (4) and a water suction pad (5); the second detection card channel comprises a second sample pad (7), a second colloidal gold pad (8), a second nitrocellulose reaction membrane (9) and a second water absorption pad (10);
sample pad (2), colloidal gold pad (3), nitrocellulose reaction membrane (4) and absorb water pad (5) paste in proper order constitute detection channel one on plastics card (6), sample pad two (7), colloidal gold pad two (8), nitrocellulose reaction membrane two (9) and absorb water pad two (10) paste in proper order constitute detection channel two on plastics card (6), colloidal gold pad (3) is the colloidal gold pad of influenza A virus antibody one and influenza B virus antibody one that contains the colloidal gold mark, colloidal gold pad two (8) is the colloidal gold pad of the novel coronavirus antibody one that contains the colloidal gold mark.
2. The dual-channel saliva test card for influenza a and b virus and novel coronavirus according to claim 1, wherein the nitrocellulose reaction membrane (4) is provided with a test line T1, a test line T2 and a quality control line C1, the nitrocellulose reaction membrane two (9) is provided with a test line T3 and a quality control line C2, the test line T1 and the test line T2 are both located on one side of the nitrocellulose reaction membrane (4) close to the colloidal gold pad (3), the test line T3 is located on one side of the nitrocellulose reaction membrane two (9) close to the colloidal gold pad two (8), the test line T1 is coated with influenza a virus antibody two, the test line T2 is coated with influenza b virus antibody two, the test line T3 is coated with novel coronavirus antibody two, the quality control line C1 is located on one side of the nitrocellulose reaction membrane (4) far from the colloidal gold pad (3), and the quality control line C2 is located on one side of the nitrocellulose reaction membrane two (9) far from the colloidal gold pad two (8), and the quality control line C1 and the polyclonal mouse C2 contain polyclonal antibody.
3. The dual-channel saliva test card for influenza A and B viruses and novel coronavirus antibodies of claim 2, wherein the influenza A virus antibody I and the influenza A virus antibody II are antibodies with different epitopes, the influenza B virus antibody I and the influenza B virus antibody II are antibodies with different epitopes, the novel coronavirus antibody I and the novel coronavirus antibody II are also antibodies with different epitopes, and the antibodies are artificially prepared antibodies.
4. The dual-channel saliva test card for influenza A and B viruses and novel coronaviruses as claimed in claim 3, wherein the test card uses saliva as a test sample.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222145897.XU CN218824309U (en) | 2022-08-16 | 2022-08-16 | Influenza A and B virus and novel coronavirus double-channel saliva detection card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222145897.XU CN218824309U (en) | 2022-08-16 | 2022-08-16 | Influenza A and B virus and novel coronavirus double-channel saliva detection card |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN218824309U true CN218824309U (en) | 2023-04-07 |
Family
ID=87259725
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202222145897.XU Active CN218824309U (en) | 2022-08-16 | 2022-08-16 | Influenza A and B virus and novel coronavirus double-channel saliva detection card |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN218824309U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116718771A (en) * | 2023-08-08 | 2023-09-08 | 北京库尔科技有限公司 | Influenza A virus, influenza B virus, respiratory syncytial virus and novel coronavirus antigen detection kit |
-
2022
- 2022-08-16 CN CN202222145897.XU patent/CN218824309U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116718771A (en) * | 2023-08-08 | 2023-09-08 | 北京库尔科技有限公司 | Influenza A virus, influenza B virus, respiratory syncytial virus and novel coronavirus antigen detection kit |
| CN116718771B (en) * | 2023-08-08 | 2023-11-03 | 北京库尔科技有限公司 | Influenza A virus, influenza B virus, respiratory syncytial virus and novel coronavirus antigen detection kit |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111537722B (en) | Respiratory tract virus sample collection and detection integrated device | |
| CN218824309U (en) | Influenza A and B virus and novel coronavirus double-channel saliva detection card | |
| CN104459142B (en) | A kind of High Virulent Newcastle Disease Virus and low virulent strain differentiate Test paper | |
| CN106168623B (en) | Respiratory Tract Adenovirus IgA antibody test strip and its detection method | |
| CN112904001B (en) | New corona and influenza A and B virus antigen joint inspection kit and preparation method and application thereof | |
| Aiello et al. | Differences in the detection of highly pathogenic avian influenza H5N1 virus in feather samples from 4-week-old and 24-week-old infected Pekin ducks (Anas platyrhynchos var. domestica) | |
| CN202433383U (en) | Joint inspection test paper for influenza A virus antigen and influenza B virus antigen | |
| CN102539755A (en) | Test strip for detecting influenza A virus antigen in secretion and preparation method thereof | |
| CN101358972B (en) | Test paper strip for detecting one or more porcine virus diarrhea disease antibody | |
| CN210347666U (en) | African swine fever antibody colloidal gold detection card | |
| CN207472897U (en) | Novel trivalent bird flu fluorescence immune chromatography test paper bar | |
| CN102721812A (en) | Indirect ELISA (enzyme-linked immuno-sorbent assay) kit for detecting nephropathogenic avian infectious bronchitis virus and antibody thereof | |
| CN116735866A (en) | Test strip for triple detection of feline panleukopenia virus, feline herpesvirus and feline calicivirus antigen and preparation method thereof | |
| CN202599960U (en) | Colloidal gold test strip for rapid detection of O-type foot-and-mouth disease virus, classical swine fever virus and porcine reproductive and respiratory syndrome virus | |
| CN204479590U (en) | Helicobacter Pylori urease antibody IgM, IgG combine device for fast detecting | |
| CN102262156B (en) | Detection test strip for coxsackie virus A16 type (CA16) IgM (Immune Globulin M) antibody | |
| CN103146651B (en) | Test strip capable of testing infectious bovine rhinotracheitis virus (IBRV) by monoclonal antibody mediated nano gold spot infiltration method | |
| CN213023172U (en) | New-crown double-hole sample adding reagent plate | |
| CN107589252A (en) | A kind of H5 subtype avian influenza virus fluorescent microsphere detection card and its application | |
| CN212008620U (en) | Detection card of novel coronavirus antigen and antibody based on nanoenzyme | |
| Purtle et al. | One year duration of immunity of the modified live bovine viral diarrhea virus type 1 and type 2 and bovine herpesvirus-1 fractions of Vista® Once SQ vaccine | |
| CN205643358U (en) | Test paper strip of short -term test chicken infectious laryngotracheitis antiviral antibody | |
| CN219142858U (en) | Detection card for rapidly detecting influenza A virus in saliva | |
| CN103941001B (en) | Haemophilus parasuis infection Rapid detection test strip | |
| CN219142859U (en) | Detection card for rapidly detecting influenza A and B virus infection |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |