CN205643358U - Test paper strip of short -term test chicken infectious laryngotracheitis antiviral antibody - Google Patents
Test paper strip of short -term test chicken infectious laryngotracheitis antiviral antibody Download PDFInfo
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- CN205643358U CN205643358U CN201620169991.0U CN201620169991U CN205643358U CN 205643358 U CN205643358 U CN 205643358U CN 201620169991 U CN201620169991 U CN 201620169991U CN 205643358 U CN205643358 U CN 205643358U
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- infectious laryngotracheitis
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Abstract
The utility model provides a test paper strip of short -term test chicken infectious laryngotracheitis antiviral antibody, includes the supporting layer, and sample absorbent fibrous layer is pasted to supporting layer one end, and the layer that absorbs water is pasted to the other end, pastes cellulose membrane layer and gold mark fibrous layer, absorbent fibrous layer, gold mark fibrous layer, cellulose membrane layer, the juncture fibre alternately infiltration mutually that absorbs water and splice each other between the layer on the absorbent fibrous layer of supporting layer and the part that absorbs water between the layer. The utility model discloses at least, including following beneficial effect: (1 )The detection specificity is strong, and sensitiveness is high. The utility model provides a test paper strip is prepared from based on the reorganization chicken infectious laryngotracheitis virus gB albumen of colloidal gold mark chicken infectious laryngotracheitis virus or colloidal gold mark, no covalent bond formation between gold grain and the viral albumen molecule in the viral albumen of gold mark, and the two combines together through the van der waals force between the charges of different polarity, and the colloidal gold mark is very little to the specificity and the affinity influence of viral albumen, and has higher trace rate. (2 )It is easy and simple to handle, quick.
Description
Technical field
This utility model relates to a kind of utensil detecting avian infectious laryngotracheitis virus antibody, particularly relates to the test strips of a kind of quick detection avian infectious laryngotracheitis virus antibody.
Background technology
Infectious laryngotracheitis of chicken (Infectious laryngotracheitis, ILT) is by avian infectious laryngotracheitis virus (Infectious
Laryngotracheitis virus, ILTV) chicken caused is acute, hot, the high degree in contact upper respiratory infectious disease, infected chicken shows as dyspnea, expectoration band blood mucus, tracheal mucosa edema and hemorrhage etc., and cause infected chicken dead and the decline of laying hen egg production, cause serious economic loss to poultry husbandry.
ILT is widely current in all over the world, has a strong impact on aviculture and develops in a healthy way.China finds primary disease in the 50's of last century, has been widely current, and is one of important epidemic disease endangering poultry husbandry.Owing to ILTV belongs to herpesvirus, energy latent infection is in nervi trigeminus cell, it is to avoid host immune is removed, thus sustainable existence in chicken group.And, latent infection chicken stressed, ILTV can be activated, and massive duplication and discharge.Therefore, chicken group once infects ILTV, just cannot remove, thus causes irregularly morbidity, significantly impacts the sound development of China's poultry husbandry.
Quickly detection ILTV antibody, is possible not only to evaluate immune effect of vaccine, and can diagnose with adjuvant clinical, be the premise effectively controlling ILT.At present the method for detection ILTV antibody mainly has in agar immunodiffusion test (AGP), serum and the method such as experiment, IFA and ELISA.But, AGP is the longest, with experiment, IFA and ELISA detection technique operation complexity in serum, instrument and equipment, process that needs are expensive are longer, time and effort consuming, need particular instrument equipment and professional and technical personnel's operation, be difficult to popularize in basic unit, it is impossible to meet production line or epidemic disease on-the-spot quickly detect needs.It would therefore be highly desirable to there be equipment and the technology of a kind of quick detection ILTV antibody.
Utility model content
A purpose of the present utility model is to provide the test strips of a kind of quick detection avian infectious laryngotracheitis virus antibody, and provides the advantage that at least will be described later.
This utility model it is also an object that provide a kind of result intuitive display, accurately, quickly detect the test strips of ILTV antibody, compared with other detection methods, this test strips high specificity, highly sensitive, easy and simple to handle, result show that quickly testing cost is cheap.
The technical solution of the utility model particularly as follows:
A kind of test strips of quick detection avian infectious laryngotracheitis virus antibody, including supporting layer, sample adsorption fibrous layer is pasted in supporting layer one end, the other end pastes water accepting layer, bonding partially cellulose membrane layer between adsorbing fiber layer and the water accepting layer of supporting layer and gold mark fibrous layer, adsorbing fiber layer, gold mark fibrous layer, cellulose membrane layer, the intersection fiber spliced each other between water accepting layer crosses one another infiltration, described cellulose membrane layer is provided with labelling and compares trace with comprising with sheep or rabbit anti-avian infectious laryngotracheitis virus antibody with the detection trace of the avian infectious laryngotracheitis virus antibody of purification, detect trace and compare trace arranged in parallel on cellulose membrane layer, that detects trace is located proximate to adsorbing fiber layer；Gold mark fibrous layer is that the glass cotton of the recombination chicken infectious laryngotracheitis virus gB albumen being attached with the avian infectious laryngotracheitis virus of colloid gold label or colloid gold label is made；It is water intake end that this test strips is provided with the fibrolaminar one end of sample adsorption, and the one end being provided with water accepting layer is holding end, and water intake end outer side covers has the first protecting film of white, and holding end is coated with the second protecting film；Being partial at sample adsorption fibrous layer side 0.55cm be printed on trace line on the protecting film that sample adsorption fibrous layer is corresponding with gold mark fibrous layer intersection, the right-hand member of trace line is printed on arrow and MAX printed words.
Holding end is provided with the groove being easy to grip, and groove extends inward into water accepting layer.
Supporting layer 1 is set to latticed at water intake end, generally circular in shape, square, triangle, rhombus, the hexagon of its mesh 11.
Supporting layer is for make with plastic slice bar.
This utility model at least includes following beneficial effect:
(1) detection high specificity, sensitivity is high.The test strips that this utility model provides is prepared from based on the recombination chicken infectious laryngotracheitis virus gB albumen of colloid gold label avian infectious laryngotracheitis virus or colloid gold label, gold secondary disease toxalbumin is formed without covalent bond between gold grain and virus protein molecule, the two is combined by the Van der Waals force between the charges of different polarity, gold colloidal mark is the least on specificity and the affinity impact of virus protein, and has higher trace rate.
(2) easy and simple to handle, quickly.Test strips of the present utility model is in use without additional any Other Instruments and reagent, as long as by its test lead insertion measuring samples liquid about 30 seconds, within about 5 minutes, i.e. can determine that testing result.
(3) result intuitive display, accurately.Test strips of the present utility model is to show that henna detection trace and comparison trace, as the positive detected and negative trace, i.e. show two brownish red traces on cellulose membrane, represent and have antiviral antibody to detect in detected sample, and result is the positive；On cellulose membrane, only one brownish red of display compares trace C, represents and does not detects antiviral antibody in detected sample liquid, and result is negative.Result judges directly perceived, accurate, simple and clear, is difficult to false negative and false positive erroneous judgement occur.
(4) investment and testing cost are reduced.Test strips of the present utility model in use, is not required to separately join Other Instruments, equipment and reagent, saves big measuring appratus, equipment and additive reagent expense；Specialty and layman all can carry out Site Detection whenever and wherever possible, it is not necessary to payment expert diagnosis Laboratory Fee or feeding sample remove the travelling expenses of diagnosis room, save testing cost, and testing cost is low.
(5) applied range, it is simple to popularity is applied.The test strips that this utility model provides is simple to operate, become " single step " or " foolproof ", and be convenient for carrying and preserve, the needs of different levels personnel can be met, including specialty chemical examination, customs quarantine control, health and epidemic prevention, quality-monitoring, livestock products processing, intensive culture to individual cultivation etc., there are wide market prospect and preferable economical, societal benefits.
(6) this utility model is according to the ultimate principle of ELISA, antiviral antibody is detected with immune chromatography test paper, utilize diafiltration concentration and the capillarity of microporous filter membrane, forward to quickly carry out on solid phase filter membrane by the Traditional liquid phase environment of ELISA by antigen-antibody reaction, and use gold colloidal trace to replace enzyme trace, with the colour developing situation of direct visual perception gold colloidal, immediately obtaining testing result, therefore the method is easier than serological methods such as ELISA, quickly.
Accompanying drawing explanation
Fig. 1 is generalized section of the present utility model；
Fig. 2 is schematic top plan view of the present utility model.
Fig. 3 is the schematic diagram of supporting layer 1.
Detailed description of the invention
Below in conjunction with the accompanying drawings this utility model is described in further detail, to make those skilled in the art can implement according to this with reference to description word.
Such as Fig. 1 and Fig. 2, a kind of test strips of quick detection avian infectious laryngotracheitis virus antibody, including supporting layer 1, sample adsorption fibrous layer 2 is pasted in supporting layer 1 one end, the other end pastes water accepting layer 5, bonding partially cellulose membrane layer 4 between the adsorbing fiber layer 2 and water accepting layer 5 of supporting layer 1 and gold mark fibrous layer 3, adsorbing fiber layer 2, gold mark fibrous layer 3, cellulose membrane layer 4, the intersection fiber spliced each other between water accepting layer 5 crosses one another infiltration, cellulose membrane layer 4 is provided with labelling and compares trace 7 with comprising with sheep or rabbit anti-avian infectious laryngotracheitis virus antibody with the detection trace 6 of the avian infectious laryngotracheitis virus antibody of purification, detect trace 6 and compare trace 7 arranged in parallel on cellulose membrane layer, that detects trace 6 is located proximate to adsorbing fiber layer, the code name of detection trace 6 is T；The code name of comparison trace 7 is C.
Gold mark fibrous layer 3 is that the glass cotton of the recombination chicken infectious laryngotracheitis virus gB albumen being attached with the avian infectious laryngotracheitis virus of colloid gold label or colloid gold label is made.
It is water intake end (also referred to as test lead) that this test strips is provided with one end of sample adsorption fibrous layer 2; the one end being provided with water accepting layer 5 is holding end; water intake end outer side covers has the first protecting film 81 of white, and holding end is coated with the second protecting film 82 of other color such as yellow.Being partial at sample adsorption fibrous layer 2 side 0.55cm be printed on trace line 9 on the protecting film that sample adsorption fibrous layer 2 is corresponding with gold mark fibrous layer 3 intersection, the right-hand member of trace line is printed on arrow and MAX printed words.
Even if it is true that be provided with the protecting film of different colours, it is also possible to make testing staff be obscured with holding end by water intake end, causing detection accident.So, the groove 83 or projection (only having drawn groove in figure) being easy to grip it is provided with at holding end, groove 83 or raised marking have thumb shape, so arrange, testing staff can be facilitated to distinguish water end (W.E.) and holding end, be also convenient for gripping, more met ergonomics.
If groove 83, groove 83 extends inward into water accepting layer 5 or supporting layer 1, convenient gripping.
If projection, projection can be elastomeric material, convenient gripping.
Supporting layer 1 is for make with plastic slice bar.
Such as Fig. 3, supporting layer 1 is set to latticed at water intake end, generally circular in shape, square, triangle, rhombus, the hexagon of its mesh 11.During normal ELISA test strip, the opposite side of water accepting layer 5 only supporting layer 1 can absorb water (solution), and the water intake end of supporting layer 1 of the present utility model is latticed, water accepting layer 5 can be made also to be absorbed water by mesh 11 in the side of supporting layer 1, since so, water accepting layer 5 can absorb water with two sides, accelerates the speed of water suction, shorten the time used by detection, improve work efficiency.
Sample adsorption fibrous layer 2 is for make with glass cotton.
Cellulose membrane layer 4 is made for nitrocellulose filter.
Water accepting layer 5 is for be made up of absorbent filter.
The detection operational approach of described test strips
The preparation of a detection sample liquid: the aseptic blood taking tested animal, and separate serum, make the dilute serum of 1:200 times with normal saline, obtain testing sample；
B detects operation: being inserted by this test strips water intake end in detected sample, insertion depth is less than trace line, takes out test strips, horizontal positioned about 1-5 minute, observed result simultaneously after about 30 seconds.
C result judges: if only demonstrating a brownish red comparison trace C on the cellulose membrane of test strips, represents that surveying inspection result is negative, and illustrates not detect avian infectious laryngotracheitis virus antibody in test sample liquid；If henna comparison trace C and detection trace T occurs in the cellulose membrane in test strips, represent that testing result is positive, in measuring samples, i.e. detect avian infectious laryngotracheitis virus antibody；If not having any brownish red trace to show on cellulose membrane, then show that test strips had lost efficacy or operated wrong.
The Cleaning Principle of above-mentioned test strips:
After the i.e. adsorbing fiber layer of test strips test lead inserts detected sample solution, solution to be checked drives the gold mark antigen in sick avian infectious laryngotracheitis virus antibody to be checked and gold mark fibrous membrane to spread to cellulose membrane together by chromatography effect, and eventually penetrate in the water accepting layer of handle end, the golden secondary disease that in diffusion process, antiviral antibody to be checked can be corresponding with this antiviral antibody is malicious or golden indicated weight histone combines, and then detect the virus combination in trace on cellulose membrane, thus demonstrate henna detection trace T；Compare the sheep in trace or rabbit anti-avian infectious laryngotracheitis virus IgG then can form brownish red comparison trace C with gold mark avian infectious laryngotracheitis virus or the recombination chicken infectious laryngotracheitis virus gB protein binding of gold labelling.If not having ILTV antibody in measuring samples liquid, test strips only demonstrates a brownish red comparison trace C；If not having any brownish red trace to show on cellulose membrane, then show that test strips had lost efficacy or operational error.
Claims (2)
- null1. the test strips of a quick detection avian infectious laryngotracheitis virus antibody，Including supporting layer (1)，Sample adsorption fibrous layer (2) is pasted in supporting layer (1) one end，The other end pastes water accepting layer (5)，Bonding partially cellulose membrane layer (4) between the adsorbing fiber layer (2) and water accepting layer (5) of supporting layer (1) and gold mark fibrous layer (3)，Adsorbing fiber layer (2)、Gold mark fibrous layer (3)、Cellulose membrane layer (4)、The intersection fiber spliced each other between water accepting layer (5) crosses one another infiltration，It is characterized in that: described cellulose membrane layer (4) is provided with labelling and compares trace (7) with comprising with sheep or rabbit anti-avian infectious laryngotracheitis virus antibody with the detection trace (6) of the avian infectious laryngotracheitis virus antibody of purification，Detect trace (6) and compare trace (7) arranged in parallel on cellulose membrane layer，That detects trace (6) is located proximate to adsorbing fiber layer；Gold mark fibrous layer (3) is that the glass cotton of the recombination chicken infectious laryngotracheitis virus gB albumen being attached with the avian infectious laryngotracheitis virus of colloid gold label or colloid gold label is made；It is water intake end that this test strips is provided with one end of sample adsorption fibrous layer (2), and the one end being provided with water accepting layer (5) is holding end, and water intake end outer side covers has first protecting film (81) of white, and holding end is coated with the second protecting film (82)；The protecting film that sample adsorption fibrous layer (2) is corresponding with gold mark fibrous layer (3) intersection is partial at sample adsorption fibrous layer (2) side 0.55cm be printed on trace line (9)；Holding end is provided with the groove (83) being easy to grip, and groove (83) extends inward into water accepting layer (5)；Supporting layer (1) is set to latticed at water intake end, its mesh (11) be shaped as triangle.
- 2. the test strips quickly detecting avian infectious laryngotracheitis virus antibody as claimed in claim 1, it is characterised in that: described supporting layer (1) is for make with plastic slice bar.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620169991.0U CN205643358U (en) | 2016-03-02 | 2016-03-02 | Test paper strip of short -term test chicken infectious laryngotracheitis antiviral antibody |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620169991.0U CN205643358U (en) | 2016-03-02 | 2016-03-02 | Test paper strip of short -term test chicken infectious laryngotracheitis antiviral antibody |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205643358U true CN205643358U (en) | 2016-10-12 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620169991.0U Expired - Fee Related CN205643358U (en) | 2016-03-02 | 2016-03-02 | Test paper strip of short -term test chicken infectious laryngotracheitis antiviral antibody |
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| Country | Link |
|---|---|
| CN (1) | CN205643358U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110146702A (en) * | 2019-06-19 | 2019-08-20 | 四川省畜牧科学研究院 | A kind of test strips detecting infectious laryngotracheitis virus, preparation method, detection method and kit |
-
2016
- 2016-03-02 CN CN201620169991.0U patent/CN205643358U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110146702A (en) * | 2019-06-19 | 2019-08-20 | 四川省畜牧科学研究院 | A kind of test strips detecting infectious laryngotracheitis virus, preparation method, detection method and kit |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20161012 Termination date: 20170302 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |